LOCUS       DACECS010000001        98288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.001, whole genome shotgun sequence.
ACCESSION   DACECS010000001 DACECS010000000
VERSION     DACECS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 98288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.001"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..1845)
                     /locus_tag="IWO31_000001"
     CDS             complement(1..1845)
                     /locus_tag="IWO31_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300459.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2057..2575
                     /locus_tag="IWO31_000002"
     CDS             2057..2575
                     /locus_tag="IWO31_000002"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0300460.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2703..3386
                     /locus_tag="IWO31_000003"
     CDS             2703..3386
                     /locus_tag="IWO31_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0300461.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3695..3768
                     /locus_tag="IWO31_000004"
     tRNA            3695..3768
                     /locus_tag="IWO31_000004"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3729..3731,aa:Arg,seq:tct)
     gene            3927..4286
                     /locus_tag="IWO31_000005"
     CDS             3927..4286
                     /locus_tag="IWO31_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0300462.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4409..6040
                     /locus_tag="IWO31_000006"
     CDS             4409..6040
                     /locus_tag="IWO31_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0300463.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6102..6743)
                     /locus_tag="IWO31_000007"
     CDS             complement(6102..6743)
                     /locus_tag="IWO31_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0300464.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7025..7333
                     /gene="rplU"
                     /locus_tag="IWO31_000008"
     CDS             7025..7333
                     /gene="rplU"
                     /locus_tag="IWO31_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0300465.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7360..7704
                     /locus_tag="IWO31_000009"
     CDS             7360..7704
                     /locus_tag="IWO31_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0300466.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7717..8010
                     /gene="rpmA"
                     /locus_tag="IWO31_000010"
     CDS             7717..8010
                     /gene="rpmA"
                     /locus_tag="IWO31_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0300467.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            8417..11251
                     /locus_tag="IWO31_000011"
     CDS             8417..11251
                     /locus_tag="IWO31_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0300468.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            11442..11933
                     /locus_tag="IWO31_000012"
     CDS             11442..11933
                     /locus_tag="IWO31_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0300469.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12036..13025
                     /gene="manX"
                     /locus_tag="IWO31_000013"
     CDS             12036..13025
                     /gene="manX"
                     /locus_tag="IWO31_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0300470.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13058..13861
                     /locus_tag="IWO31_000014"
     CDS             13058..13861
                     /locus_tag="IWO31_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0300471.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            13882..14796
                     /locus_tag="IWO31_000015"
     CDS             13882..14796
                     /locus_tag="IWO31_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0300472.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            14879..15253
                     /locus_tag="IWO31_000016"
     CDS             14879..15253
                     /locus_tag="IWO31_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0300473.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            15392..15637
                     /locus_tag="IWO31_000017"
     CDS             15392..15637
                     /locus_tag="IWO31_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0300474.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            15809..16873
                     /locus_tag="IWO31_000018"
     CDS             15809..16873
                     /locus_tag="IWO31_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0300475.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17119..17544
                     /locus_tag="IWO31_000019"
     CDS             17119..17544
                     /locus_tag="IWO31_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0300476.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            17537..17986
                     /gene="nusB"
                     /locus_tag="IWO31_000020"
     CDS             17537..17986
                     /gene="nusB"
                     /locus_tag="IWO31_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0300477.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18083..18937
                     /locus_tag="IWO31_000021"
     CDS             18083..18937
                     /locus_tag="IWO31_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0300478.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            18938..20275
                     /locus_tag="IWO31_000022"
     CDS             18938..20275
                     /locus_tag="IWO31_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0300479.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20280..20504
                     /locus_tag="IWO31_000023"
     CDS             20280..20504
                     /locus_tag="IWO31_000023"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0300480.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            20506..21396
                     /locus_tag="IWO31_000024"
     CDS             20506..21396
                     /locus_tag="IWO31_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0300481.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            21405..22223
                     /locus_tag="IWO31_000025"
     CDS             21405..22223
                     /locus_tag="IWO31_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0300482.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            22330..22779
                     /locus_tag="IWO31_000026"
     CDS             22330..22779
                     /locus_tag="IWO31_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0300483.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            22797..24479
                     /gene="recN"
                     /locus_tag="IWO31_000027"
     CDS             22797..24479
                     /gene="recN"
                     /locus_tag="IWO31_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0300484.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(24534..24650)
                     /locus_tag="IWO31_000028"
     CDS             complement(24534..24650)
                     /locus_tag="IWO31_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0300485.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(24723..25679)
                     /locus_tag="IWO31_000029"
     CDS             complement(24723..25679)
                     /locus_tag="IWO31_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0300486.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            25850..26218
                     /locus_tag="IWO31_000030"
     CDS             25850..26218
                     /locus_tag="IWO31_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0300487.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            26388..26819
                     /gene="mraZ"
                     /locus_tag="IWO31_000031"
     CDS             26388..26819
                     /gene="mraZ"
                     /locus_tag="IWO31_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0300488.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            26840..27799
                     /gene="rsmH"
                     /locus_tag="IWO31_000032"
     CDS             26840..27799
                     /gene="rsmH"
                     /locus_tag="IWO31_000032"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0300489.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            27804..28205
                     /gene="ftsL"
                     /locus_tag="IWO31_000033"
     CDS             27804..28205
                     /gene="ftsL"
                     /locus_tag="IWO31_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0300490.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            28206..30398
                     /locus_tag="IWO31_000034"
     CDS             28206..30398
                     /locus_tag="IWO31_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0300491.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            30424..31386
                     /locus_tag="IWO31_000035"
     CDS             30424..31386
                     /locus_tag="IWO31_000035"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0300492.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            31400..32761
                     /locus_tag="IWO31_000036"
     CDS             31400..32761
                     /locus_tag="IWO31_000036"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0300493.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            32787..33875
                     /gene="murG"
                     /locus_tag="IWO31_000037"
     CDS             32787..33875
                     /gene="murG"
                     /locus_tag="IWO31_000037"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0300494.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            33890..35110
                     /locus_tag="IWO31_000038"
     CDS             33890..35110
                     /locus_tag="IWO31_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0300495.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35266..36591
                     /gene="ftsA"
                     /locus_tag="IWO31_000039"
     CDS             35266..36591
                     /gene="ftsA"
                     /locus_tag="IWO31_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0300496.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            36614..37855
                     /gene="ftsZ"
                     /locus_tag="IWO31_000040"
     CDS             36614..37855
                     /gene="ftsZ"
                     /locus_tag="IWO31_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0300497.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            37872..38549
                     /locus_tag="IWO31_000041"
     CDS             37872..38549
                     /locus_tag="IWO31_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0300498.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            38563..39162
                     /locus_tag="IWO31_000042"
     CDS             38563..39162
                     /locus_tag="IWO31_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0300499.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39177..39464
                     /locus_tag="IWO31_000043"
     CDS             39177..39464
                     /locus_tag="IWO31_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0300500.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            39513..40295
                     /locus_tag="IWO31_000044"
     CDS             39513..40295
                     /locus_tag="IWO31_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0300501.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            40316..41023
                     /locus_tag="IWO31_000045"
     CDS             40316..41023
                     /locus_tag="IWO31_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0300502.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41285..44083
                     /gene="ileS"
                     /locus_tag="IWO31_000046"
     CDS             41285..44083
                     /gene="ileS"
                     /locus_tag="IWO31_000046"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0300503.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44132..45658)
                     /locus_tag="IWO31_000047"
     CDS             complement(44132..45658)
                     /locus_tag="IWO31_000047"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0300504.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(45673..46320)
                     /locus_tag="IWO31_000048"
     CDS             complement(45673..46320)
                     /locus_tag="IWO31_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0300505.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(46504..46833)
                     /locus_tag="IWO31_000049"
     CDS             complement(46504..46833)
                     /locus_tag="IWO31_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0300506.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47010..47738
                     /gene="sfsA"
                     /locus_tag="IWO31_000050"
     CDS             47010..47738
                     /gene="sfsA"
                     /locus_tag="IWO31_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0300507.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            47754..48767
                     /locus_tag="IWO31_000051"
     CDS             47754..48767
                     /locus_tag="IWO31_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0300508.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            48767..50044
                     /locus_tag="IWO31_000052"
     CDS             48767..50044
                     /locus_tag="IWO31_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0300509.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50107..52809
                     /locus_tag="IWO31_000053"
     CDS             50107..52809
                     /locus_tag="IWO31_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0300510.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            52961..53278
                     /locus_tag="IWO31_000054"
     CDS             52961..53278
                     /locus_tag="IWO31_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0300511.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            53308..53628
                     /locus_tag="IWO31_000055"
     CDS             53308..53628
                     /locus_tag="IWO31_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0300512.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            53736..55196
                     /locus_tag="IWO31_000056"
     CDS             53736..55196
                     /locus_tag="IWO31_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0300513.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55264..55485
                     /locus_tag="IWO31_000057"
     CDS             55264..55485
                     /locus_tag="IWO31_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300514.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            55516..55698
                     /locus_tag="IWO31_000058"
     CDS             55516..55698
                     /locus_tag="IWO31_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0300515.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            55698..56111
                     /locus_tag="IWO31_000059"
     CDS             55698..56111
                     /locus_tag="IWO31_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0300516.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56234..57415
                     /locus_tag="IWO31_000060"
     CDS             56234..57415
                     /locus_tag="IWO31_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0300517.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            57804..57877
                     /locus_tag="IWO31_000061"
     tRNA            57804..57877
                     /locus_tag="IWO31_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57838..57840,aa:Arg,seq:cct)
     gene            complement(57914..58114)
                     /locus_tag="IWO31_000062"
     CDS             complement(57914..58114)
                     /locus_tag="IWO31_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0300518.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(58419..59651)
                     /locus_tag="IWO31_000063"
     CDS             complement(58419..59651)
                     /locus_tag="IWO31_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0300519.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            59905..60474
                     /locus_tag="IWO31_000064"
     CDS             59905..60474
                     /locus_tag="IWO31_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0300520.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(60652..61092)
                     /locus_tag="IWO31_000065"
     CDS             complement(60652..61092)
                     /locus_tag="IWO31_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0300521.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            61250..62014
                     /gene="map"
                     /locus_tag="IWO31_000066"
     CDS             61250..62014
                     /gene="map"
                     /locus_tag="IWO31_000066"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0300522.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            62046..62969
                     /locus_tag="IWO31_000067"
     CDS             62046..62969
                     /locus_tag="IWO31_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0300523.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            63045..64184
                     /locus_tag="IWO31_000068"
     CDS             63045..64184
                     /locus_tag="IWO31_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0300524.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            64177..64977
                     /locus_tag="IWO31_000069"
     CDS             64177..64977
                     /locus_tag="IWO31_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0300525.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            64977..65804
                     /locus_tag="IWO31_000070"
     CDS             64977..65804
                     /locus_tag="IWO31_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0300526.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            65782..66516
                     /locus_tag="IWO31_000071"
     CDS             65782..66516
                     /locus_tag="IWO31_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0300527.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            66616..67482
                     /gene="rfbA"
                     /locus_tag="IWO31_000072"
     CDS             66616..67482
                     /gene="rfbA"
                     /locus_tag="IWO31_000072"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0300528.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            67496..68068
                     /gene="rfbC"
                     /locus_tag="IWO31_000073"
     CDS             67496..68068
                     /gene="rfbC"
                     /locus_tag="IWO31_000073"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0300529.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            68090..69118
                     /gene="rfbB"
                     /locus_tag="IWO31_000074"
     CDS             68090..69118
                     /gene="rfbB"
                     /locus_tag="IWO31_000074"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0300530.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            69216..70067
                     /gene="rfbD"
                     /locus_tag="IWO31_000075"
     CDS             69216..70067
                     /gene="rfbD"
                     /locus_tag="IWO31_000075"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0300531.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            70102..72135
                     /locus_tag="IWO31_000076"
     CDS             70102..72135
                     /locus_tag="IWO31_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300532.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            72179..73459
                     /locus_tag="IWO31_000077"
     CDS             72179..73459
                     /locus_tag="IWO31_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0300533.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            73669..74475
                     /locus_tag="IWO31_000078"
     CDS             73669..74475
                     /locus_tag="IWO31_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0300534.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            74487..75707
                     /locus_tag="IWO31_000079"
     CDS             74487..75707
                     /locus_tag="IWO31_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300535.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            75697..77283
                     /locus_tag="IWO31_000080"
     CDS             75697..77283
                     /locus_tag="IWO31_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0300536.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            77322..79460
                     /locus_tag="IWO31_000081"
     CDS             77322..79460
                     /locus_tag="IWO31_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0300537.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(79828..80811)
                     /locus_tag="IWO31_000082"
     CDS             complement(79828..80811)
                     /locus_tag="IWO31_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0300538.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            81189..82580
                     /locus_tag="IWO31_000083"
     CDS             81189..82580
                     /locus_tag="IWO31_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0300539.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            82596..83636
                     /locus_tag="IWO31_000084"
     CDS             82596..83636
                     /locus_tag="IWO31_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0300540.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            83655..84740
                     /locus_tag="IWO31_000085"
     CDS             83655..84740
                     /locus_tag="IWO31_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0300541.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            84773..85735
                     /locus_tag="IWO31_000086"
     CDS             84773..85735
                     /locus_tag="IWO31_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0300542.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            85728..87155
                     /locus_tag="IWO31_000087"
     CDS             85728..87155
                     /locus_tag="IWO31_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0300543.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            87157..88227
                     /locus_tag="IWO31_000088"
     CDS             87157..88227
                     /locus_tag="IWO31_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300544.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            88214..89635
                     /locus_tag="IWO31_000089"
     CDS             88214..89635
                     /locus_tag="IWO31_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0300545.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            89648..90655
                     /locus_tag="IWO31_000090"
     CDS             89648..90655
                     /locus_tag="IWO31_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0300546.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            90667..91671
                     /gene="neuB"
                     /locus_tag="IWO31_000091"
     CDS             90667..91671
                     /gene="neuB"
                     /locus_tag="IWO31_000091"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0300547.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            91668..92816
                     /gene="neuC"
                     /locus_tag="IWO31_000092"
     CDS             91668..92816
                     /gene="neuC"
                     /locus_tag="IWO31_000092"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0300548.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            92789..93466
                     /locus_tag="IWO31_000093"
     CDS             92789..93466
                     /locus_tag="IWO31_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0300549.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            93456..94598
                     /locus_tag="IWO31_000094"
     CDS             93456..94598
                     /locus_tag="IWO31_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0300550.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            94611..95588
                     /locus_tag="IWO31_000095"
     CDS             94611..95588
                     /locus_tag="IWO31_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0300551.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            95588..96496
                     /locus_tag="IWO31_000096"
     CDS             95588..96496
                     /locus_tag="IWO31_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0300552.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVTTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            96497..97249
                     /locus_tag="IWO31_000097"
     CDS             96497..97249
                     /locus_tag="IWO31_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0300553.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            97254..98201
                     /locus_tag="IWO31_000098"
     CDS             97254..98201
                     /locus_tag="IWO31_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0300554.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        32614 a        15922 c        20305 g        29447 t
ORIGIN      
        1 ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc
       61 tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca tcattaagtc
      121 aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt ttgtatcgta
      181 atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta tcgacaaata
      241 aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat gctctaaagc
      301 gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg gaagatctgc
      361 ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg ctaagataag
      421 atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg cttcttctaa
      481 tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat cttgcagatt
      541 ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat gctgtcgaag
      601 ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta cttgtctccg
      661 atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa cgattagaat
      721 gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta atgccgtttg
      781 gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac aagctacata
      841 aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta aagtgcttat
      901 aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag taaaagttgc
      961 ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc cgtaccattt
     1021 tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg ttaagataat
     1081 aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac ttaaaatatc
     1141 ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa taagtgagta
     1201 ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc ctatagcgat
     1261 aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa taaaactaat
     1321 caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt tctgataagg
     1381 agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat ctatcacagc
     1441 caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata aaatcttttc
     1501 gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg cttcgtccaa
     1561 cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg taggaatcgt
     1621 acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga aaacccacga
     1681 atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat aaaagaaata
     1741 agcaattcct gttaaaaata acaatgataa tataatgatt atgctccact ttatacgtaa
     1801 ataaaaagat tcttttttat tatatatgtt attaaaaaaa gacataaagg tattcctttc
     1861 taaaacttgt aatgttaggg taaacgtttt ttaaactaac gtcaattatt tataataaaa
     1921 aatgagataa agatgaggaa atagaattag aaacatataa ttattaaagg gaaacatttt
     1981 tacagctata atttgaaaaa aatttatcgt tattatatga ttaagaagga ttgaataaag
     2041 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
     2101 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
     2161 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
     2221 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
     2281 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
     2341 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
     2401 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
     2461 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
     2521 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
     2581 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
     2641 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
     2701 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
     2761 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
     2821 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     2881 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     2941 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     3001 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     3061 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     3121 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     3181 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     3241 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     3301 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     3361 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     3421 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     3481 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     3541 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     3601 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     3661 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     3721 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     3781 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     3841 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     3901 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     3961 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     4021 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     4081 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     4141 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     4201 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     4261 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     4321 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     4381 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     4441 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     4501 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     4561 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     4621 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     4681 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     4741 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     4801 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     4861 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     4921 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     4981 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     5041 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     5101 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     5161 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     5221 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     5281 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     5341 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     5401 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     5461 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     5521 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     5581 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     5641 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     5701 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     5761 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     5821 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     5881 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     5941 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     6001 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     6061 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     6121 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     6181 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     6241 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     6301 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     6361 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     6421 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     6481 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     6541 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     6601 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     6661 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     6721 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     6781 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     6841 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     6901 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     6961 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     7021 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     7081 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     7141 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     7201 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     7261 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     7321 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     7381 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     7441 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     7501 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     7561 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     7621 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     7681 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     7741 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     7801 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     7861 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     7921 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     7981 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     8041 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     8101 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     8161 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     8221 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     8281 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     8341 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     8401 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     8461 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     8521 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     8581 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     8641 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     8701 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     8761 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     8821 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     8881 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     8941 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     9001 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     9061 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     9121 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     9181 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     9241 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     9301 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     9361 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     9421 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     9481 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     9541 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     9601 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     9661 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     9721 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     9781 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     9841 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     9901 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     9961 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
    10021 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
    10081 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
    10141 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
    10201 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
    10261 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
    10321 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
    10381 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
    10441 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
    10501 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
    10561 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
    10621 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
    10681 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
    10741 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
    10801 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
    10861 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
    10921 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
    10981 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
    11041 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
    11101 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
    11161 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
    11221 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
    11281 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
    11341 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
    11401 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
    11461 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
    11521 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
    11581 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
    11641 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
    11701 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
    11761 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
    11821 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
    11881 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
    11941 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
    12001 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    12061 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    12121 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    12181 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    12241 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    12301 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    12361 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    12421 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    12481 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    12541 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    12601 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    12661 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    12721 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    12781 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    12841 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    12901 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    12961 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    13021 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    13081 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    13141 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    13201 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    13261 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    13321 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    13381 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    13441 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    13501 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    13561 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    13621 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    13681 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    13741 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    13801 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    13861 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    13921 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    13981 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    14041 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    14101 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    14161 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    14221 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    14281 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    14341 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    14401 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    14461 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    14521 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    14581 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    14641 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    14701 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    14761 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    14821 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    14881 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    14941 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    15001 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    15061 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    15121 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    15181 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    15241 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    15301 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    15361 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    15421 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    15481 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    15541 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    15601 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    15661 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    15721 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    15781 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    15841 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    15901 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    15961 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    16021 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    16081 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    16141 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    16201 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    16261 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    16321 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    16381 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    16441 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    16501 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    16561 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    16621 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    16681 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    16741 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    16801 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    16861 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    16921 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    16981 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    17041 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    17101 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    17161 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    17221 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    17281 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    17341 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    17401 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    17461 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    17521 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    17581 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    17641 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    17701 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    17761 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    17821 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    17881 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    17941 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    18001 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    18061 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    18121 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    18181 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    18241 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    18301 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    18361 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    18421 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    18481 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    18541 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    18601 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    18661 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    18721 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    18781 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    18841 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    18901 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    18961 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    19021 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    19081 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    19141 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    19201 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    19261 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    19321 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    19381 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    19441 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    19501 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    19561 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    19621 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    19681 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    19741 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    19801 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    19861 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    19921 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    19981 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    20041 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    20101 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    20161 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    20221 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    20281 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    20341 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    20401 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    20461 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    20521 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    20581 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    20641 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    20701 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    20761 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    20821 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    20881 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    20941 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    21001 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    21061 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    21121 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    21181 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    21241 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    21301 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    21361 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    21421 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    21481 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    21541 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    21601 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    21661 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    21721 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    21781 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    21841 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    21901 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    21961 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    22021 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    22081 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    22141 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    22201 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    22261 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    22321 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    22381 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    22441 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    22501 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    22561 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    22621 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    22681 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    22741 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    22801 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    22861 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    22921 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    22981 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    23041 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    23101 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    23161 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    23221 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    23281 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    23341 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    23401 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    23461 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    23521 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    23581 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    23641 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    23701 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    23761 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    23821 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    23881 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    23941 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    24001 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    24061 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    24121 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    24181 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    24241 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    24301 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    24361 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    24421 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    24481 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    24541 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    24601 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    24661 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    24721 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    24781 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    24841 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    24901 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    24961 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    25021 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    25081 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    25141 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    25201 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    25261 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    25321 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    25381 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    25441 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    25501 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    25561 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    25621 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    25681 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    25741 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    25801 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    25861 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    25921 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    25981 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    26041 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    26101 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    26161 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    26221 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    26281 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    26341 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    26401 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    26461 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    26521 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    26581 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    26641 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    26701 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    26761 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    26821 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    26881 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    26941 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    27001 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    27061 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    27121 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    27181 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    27241 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    27301 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    27361 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    27421 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    27481 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    27541 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    27601 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    27661 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    27721 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    27781 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    27841 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    27901 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    27961 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    28021 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    28081 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    28141 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    28201 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    28261 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    28321 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    28381 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    28441 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    28501 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    28561 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    28621 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    28681 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    28741 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    28801 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    28861 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    28921 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    28981 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    29041 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    29101 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    29161 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    29221 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    29281 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    29341 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    29401 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    29461 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    29521 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    29581 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    29641 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    29701 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    29761 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    29821 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    29881 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    29941 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    30001 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    30061 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    30121 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    30181 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    30241 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    30301 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    30361 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    30421 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    30481 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    30541 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    30601 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    30661 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    30721 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    30781 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    30841 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    30901 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    30961 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    31021 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    31081 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    31141 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    31201 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    31261 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    31321 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    31381 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    31441 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    31501 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    31561 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    31621 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    31681 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    31741 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    31801 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    31861 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    31921 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    31981 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    32041 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    32101 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    32161 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    32221 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    32281 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    32341 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    32401 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    32461 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    32521 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    32581 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    32641 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    32701 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    32761 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    32821 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    32881 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    32941 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    33001 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    33061 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    33121 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    33181 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    33241 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    33301 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    33361 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    33421 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    33481 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    33541 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    33601 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    33661 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    33721 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    33781 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    33841 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    33901 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    33961 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    34021 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    34081 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    34141 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    34201 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    34261 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    34321 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    34381 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    34441 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    34501 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    34561 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    34621 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    34681 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    34741 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    34801 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    34861 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    34921 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    34981 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    35041 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    35101 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    35161 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    35221 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    35281 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    35341 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    35401 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    35461 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    35521 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    35581 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    35641 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    35701 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    35761 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    35821 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    35881 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    35941 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    36001 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    36061 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    36121 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    36181 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    36241 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    36301 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    36361 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    36421 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    36481 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    36541 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    36601 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    36661 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    36721 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    36781 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    36841 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    36901 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    36961 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    37021 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    37081 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    37141 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    37201 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    37261 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    37321 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    37381 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    37441 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    37501 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    37561 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    37621 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    37681 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    37741 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    37801 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    37861 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    37921 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    37981 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    38041 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    38101 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    38161 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    38221 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    38281 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    38341 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    38401 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    38461 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    38521 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    38581 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    38641 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    38701 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    38761 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    38821 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    38881 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    38941 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    39001 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    39061 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    39121 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    39181 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    39241 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    39301 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    39361 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    39421 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    39481 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    39541 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    39601 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    39661 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    39721 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    39781 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    39841 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    39901 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    39961 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    40021 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    40081 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    40141 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    40201 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    40261 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    40321 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    40381 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    40441 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    40501 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    40561 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    40621 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    40681 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    40741 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    40801 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    40861 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    40921 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    40981 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    41041 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    41101 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    41161 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    41221 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    41281 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    41341 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    41401 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    41461 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    41521 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    41581 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    41641 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    41701 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    41761 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    41821 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    41881 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    41941 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    42001 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    42061 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    42121 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    42181 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    42241 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    42301 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    42361 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    42421 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    42481 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    42541 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    42601 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    42661 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    42721 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    42781 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    42841 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    42901 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    42961 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    43021 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    43081 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    43141 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    43201 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    43261 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    43321 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    43381 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    43441 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    43501 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    43561 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    43621 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    43681 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    43741 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    43801 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    43861 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    43921 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    43981 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    44041 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    44101 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    44161 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    44221 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    44281 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    44341 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    44401 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    44461 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    44521 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    44581 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    44641 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    44701 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    44761 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    44821 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    44881 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    44941 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    45001 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    45061 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    45121 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    45181 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    45241 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    45301 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    45361 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    45421 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    45481 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    45541 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    45601 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    45661 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    45721 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    45781 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    45841 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    45901 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    45961 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    46021 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    46081 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    46141 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    46201 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    46261 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    46321 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    46381 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    46441 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    46501 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    46561 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    46621 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    46681 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    46741 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    46801 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    46861 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    46921 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    46981 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    47041 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    47101 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    47161 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    47221 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    47281 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    47341 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    47401 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    47461 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    47521 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    47581 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    47641 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    47701 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    47761 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    47821 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    47881 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    47941 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    48001 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    48061 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    48121 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    48181 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    48241 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    48301 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    48361 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    48421 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    48481 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    48541 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    48601 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    48661 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    48721 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    48781 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    48841 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    48901 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    48961 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    49021 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    49081 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    49141 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    49201 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    49261 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    49321 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    49381 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    49441 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    49501 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    49561 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    49621 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    49681 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    49741 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    49801 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    49861 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    49921 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    49981 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    50041 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    50101 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    50161 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    50221 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    50281 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    50341 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    50401 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    50461 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    50521 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    50581 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    50641 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    50701 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    50761 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    50821 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    50881 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    50941 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    51001 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    51061 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    51121 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    51181 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    51241 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    51301 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    51361 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    51421 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    51481 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    51541 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    51601 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    51661 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    51721 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    51781 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    51841 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    51901 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    51961 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    52021 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    52081 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    52141 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    52201 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    52261 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    52321 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    52381 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    52441 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    52501 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    52561 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    52621 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    52681 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    52741 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    52801 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    52861 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    52921 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    52981 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    53041 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    53101 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    53161 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    53221 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    53281 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    53341 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    53401 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    53461 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    53521 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    53581 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    53641 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    53701 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    53761 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    53821 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    53881 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    53941 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    54001 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    54061 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    54121 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    54181 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    54241 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    54301 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    54361 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    54421 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    54481 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    54541 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    54601 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    54661 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    54721 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    54781 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    54841 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    54901 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    54961 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    55021 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    55081 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    55141 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    55201 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    55261 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    55321 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    55381 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    55441 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    55501 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    55561 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    55621 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    55681 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    55741 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    55801 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    55861 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    55921 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    55981 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    56041 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    56101 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    56161 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    56221 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    56281 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    56341 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    56401 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    56461 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    56521 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    56581 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    56641 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    56701 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    56761 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    56821 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    56881 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    56941 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    57001 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    57061 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    57121 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    57181 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    57241 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    57301 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    57361 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    57421 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    57481 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    57541 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    57601 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    57661 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    57721 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    57781 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    57841 aagcgatagg tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt
    57901 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
    57961 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
    58021 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
    58081 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
    58141 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
    58201 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
    58261 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
    58321 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
    58381 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
    58441 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
    58501 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
    58561 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
    58621 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
    58681 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
    58741 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
    58801 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
    58861 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
    58921 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
    58981 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
    59041 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
    59101 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
    59161 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
    59221 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
    59281 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
    59341 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
    59401 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
    59461 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
    59521 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
    59581 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
    59641 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
    59701 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
    59761 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
    59821 taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata
    59881 aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt
    59941 ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac
    60001 atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat
    60061 gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact
    60121 gttgagcata cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga
    60181 agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg
    60241 cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat
    60301 ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc
    60361 gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc
    60421 caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac
    60481 tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt
    60541 tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa
    60601 aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt
    60661 tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag
    60721 atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa
    60781 atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta
    60841 aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg
    60901 cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc
    60961 atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc
    61021 ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt
    61081 tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga attataccaa
    61141 aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg
    61201 tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt
    61261 aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca
    61321 taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt
    61381 cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga
    61441 atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc
    61501 tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc
    61561 tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga
    61621 agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg
    61681 tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga
    61741 ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg
    61801 agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt
    61861 taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga
    61921 cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat
    61981 tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga
    62041 ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga
    62101 cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt
    62161 atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc
    62221 acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc
    62281 agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg
    62341 tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga
    62401 tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac
    62461 taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg
    62521 gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc
    62581 agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga
    62641 tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct
    62701 tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg
    62761 caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta
    62821 attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg
    62881 tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga
    62941 aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca
    63001 gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt gtttctttta
    63061 gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca
    63121 aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag
    63181 ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat
    63241 tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag
    63301 taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta
    63361 tagggatctt gattgcttct cttattattt attttgtagc agatatacgt attgattcct
    63421 tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt
    63481 tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg
    63541 cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg
    63601 cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt
    63661 ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag
    63721 ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga
    63781 tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga
    63841 gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa
    63901 tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt
    63961 tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc gtattgatta
    64021 ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac
    64081 atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa
    64141 aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg
    64201 atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt
    64261 atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt
    64321 cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt
    64381 catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc tagcgattct
    64441 cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg
    64501 gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa
    64561 gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc
    64621 tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc
    64681 cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta
    64741 gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt
    64801 gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc
    64861 ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc
    64921 gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga
    64981 aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt
    65041 atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata
    65101 gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc
    65161 cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa
    65221 cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat
    65281 tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga
    65341 gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag
    65401 aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg
    65461 tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc
    65521 attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa
    65581 tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta
    65641 ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc
    65701 gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct
    65761 ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt
    65821 tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct
    65881 cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg
    65941 gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt
    66001 caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt
    66061 ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt
    66121 gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg
    66181 caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat
    66241 gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat
    66301 attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga
    66361 aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg
    66421 agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt
    66481 acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata
    66541 tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa
    66601 ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac
    66661 cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat
    66721 tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca
    66781 caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc
    66841 gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa
    66901 tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt
    66961 ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat
    67021 catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg
    67081 tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt
    67141 tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta
    67201 gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg
    67261 ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc
    67321 tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct
    67381 taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa
    67441 aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa
    67501 agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca
    67561 tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata
    67621 tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca
    67681 tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt
    67741 agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat
    67801 cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt
    67861 tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc
    67921 agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc
    67981 aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga
    68041 aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat
    68101 cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa
    68161 tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa
    68221 cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga
    68281 attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca
    68341 taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta
    68401 tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga
    68461 ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga
    68521 aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg
    68581 ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa
    68641 ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac
    68701 gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg
    68761 gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg
    68821 cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat
    68881 cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca
    68941 cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga
    69001 gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga
    69061 ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata
    69121 cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc
    69181 ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg
    69241 gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa
    69301 cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat
    69361 taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag
    69421 aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag
    69481 caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa
    69541 acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta
    69601 cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa
    69661 cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg
    69721 aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta
    69781 ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt
    69841 tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag
    69901 atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt
    69961 attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag
    70021 aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc
    70081 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
    70141 ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat
    70201 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
    70261 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
    70321 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
    70381 caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg
    70441 atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg
    70501 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
    70561 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta
    70621 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
    70681 catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc
    70741 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
    70801 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
    70861 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
    70921 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
    70981 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
    71041 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
    71101 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
    71161 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
    71221 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
    71281 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
    71341 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
    71401 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
    71461 tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt
    71521 gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac
    71581 ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
    71641 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
    71701 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
    71761 aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg
    71821 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
    71881 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
    71941 gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac
    72001 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
    72061 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
    72121 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt
    72181 gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
    72241 tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt
    72301 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
    72361 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
    72421 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt
    72481 tcctttggtt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
    72541 cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac
    72601 agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat
    72661 ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg
    72721 atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc
    72781 agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt
    72841 tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc
    72901 ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa
    72961 agtcaatttg tttaccgatc gagaagatat ttggcataaa ttctatcaga cgctaggaga
    73021 aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt
    73081 tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg
    73141 gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc
    73201 caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga
    73261 ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt
    73321 tcatcgatat gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga
    73381 cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag
    73441 gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc
    73501 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
    73561 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
    73621 aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat
    73681 ttgtatttat ttatgaagga catttatcag aacagaaaat tattgctaca gttttcgatc
    73741 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
    73801 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
    73861 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
    73921 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
    73981 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
    74041 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
    74101 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
    74161 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
    74221 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
    74281 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
    74341 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
    74401 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
    74461 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
    74521 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
    74581 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
    74641 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
    74701 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
    74761 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
    74821 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
    74881 ttgaatttgc tgacatcgga gaccatattt atcaacctgt aaaaacttat tcaagcggga
    74941 tgttcgttcg tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg
    75001 atgaagcatt ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa aaatttacag
    75061 aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatatcagt tcgattcgtc
    75121 gtttttgtga tcgttcctat tggttgaaaa acggagaagt cgtcgaatat ggcgatacga
    75181 tggaagtaac gacgaattat gaaaactttt taaagaaaaa atcagttaaa acagtggatc
    75241 ggaataaaaa ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg
    75301 aattgttgga taaagacaaa aatccggtgg atatgctgga acaaggagaa aaactatacg
    75361 ttaaagtaac ttatgaagta aaagatgata caatcaaaaa tccggtttta ggaattgcgc
    75421 tacgaacagt cgacaatttt tatgtatgcg gacttaatac tctactagat ggtatcaaga
    75481 ttccttggaa aaaaggagag aatatctttt atctggagta tgaaaagtta ggtttgttag
    75541 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
    75601 ataagacaaa gtacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
    75661 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
    75721 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
    75781 ttagagtttg gtcccggaaa cggccgatta acgaaacatt taatcggagc aaaacaatgt
    75841 gaagtgagca ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga atttgctcaa
    75901 gatggatttt atggtgatat cgaaagtttt gagtgggcaa actattatgc tggacagaca
    75961 tttgattacg tcttgtttgc ggatgtttta gaacatttgg tagatcctgg aaaaacattg
    76021 aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaacttg
    76081 gcgcataatt ctgtcatgat cgatttgttc aataaccaat tgccgtgggc ttcttatggt
    76141 ctcctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa
    76201 aaagcgggat tattcattaa tattgaagat tatttgtact tagctgtagg tgatacagaa
    76261 ttaaaatcaa cttatgaaga attgccggaa gctgtacgct atgatttcaa aatgcgacca
    76321 tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagc acaaagttcc
    76381 attgcagagc cacaaaattc taattatgta aaagtgttag aagtaaccca gcaaacgaaa
    76441 caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa tgagacacta
    76501 agtttccccg tatcagaaac aacagaaaga atggtgtttc gcttcgcgca acaacctagc
    76561 tttatcgaat tcagcgcaga agctgccgga gaaaaactaa catttatcga ttcgaatgct
    76621 gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaattacc agagttcgtt
    76681 ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa
    76741 ttgacaccaa caatgaaaga attgttggaa acgattcgtc ccatggctga agtcacaaag
    76801 cagctttctg agaaaaatga cgaattagaa agagaaaatc atcatcttct tgaggaaaat
    76861 acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt
    76921 gatgaatgga caataaaaca tagactagga cgacgtaaga aagaaacttc gaaaaaaatc
    76981 caagaaaaag aactttctat atgtattgac gagaaaatct gggatgcaga aacaaaaata
    77041 ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agcccttgtc ctataagtta
    77101 tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagaaga agtcaatcaa
    77161 gcagaacagc tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa
    77221 gaacgttcat tcttagtgga agcagtagct caaaatggcc aatcatggat cattgaaatg
    77281 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
    77341 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
    77401 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
    77461 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
    77521 agacccaaag cggtttcaag gtacgtttat cagggaaaat gagaggaaaa gccatcttag
    77581 acttccaaac agcgagacat caaaatggca tagctgtgaa attaaacggt aaataccctt
    77641 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
    77701 attatgcaag gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc
    77761 caggatcgat cgactatgca gagtggatca gtcgtcatga gaaaccagat ttgaaagagc
    77821 aaaggaaaga agcacaagga tttgcgtacc gtccattgat ttccatactt atgcctgtct
    77881 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtactggat caaacgtatg
    77941 atcattggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
    78001 tggaatccta tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac
    78061 acatcagtct ggcaactaat tctgcattgg aaatagcaga aggagaattt atagcactct
    78121 tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaacg
    78181 tacatccaga actggatgtg atctacagtg acgaagataa aatcgatgca gatggcaatc
    78241 gctttgatcc gcatttcaaa gcagactggt cgcctgatac attgatggga aataattata
    78301 tctctcactt aggcgtatat cgaacaagca ttgttaaaga acttggcggt ttccgtaaag
    78361 gctatgaagg ttctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag
    78421 atcatattta ccacattgac cgagtcttgt atcattggcg gacgatccca ggatcaactg
    78481 caagtaatgg cgaagccaaa agttatatct atgattccgg agtgaaagca ttgacagatg
    78541 ccctgtctag aagaaacatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg
    78601 agattgctta tgacgtcttg caagaggatt tggtcagtgt gatcattcca acgaagaatg
    78661 gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt
    78721 atgaaatcat tattgcggat aacggaagta cagatccaaa aatgcaagaa ttatttgcag
    78781 aatacaagca tcagctgaaa gaccgcttta tcgtagaatt gattgacatt ccattcaatt
    78841 attctcgaat taacaattta gcagcagaaa aagcaaacgg caaatacttt ttattcttga
    78901 ataacgatac agaagtgatt gagccagatt ggatgacggt gatggtttct tatgcacagt
    78961 ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg
    79021 caggagtatt attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa
    79081 cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
    79141 cttgtatgat ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag
    79201 aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg
    79261 tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata
    79321 ctccagaaaa acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag
    79381 catatattgc gcatgatcca ttttacaatg ataatttaac aaaagacgga atcggcgact
    79441 tttcgttgag accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca
    79501 taacggcttt ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa
    79561 gtcgaggttg ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc
    79621 ttttcaaatt tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt
    79681 tattcgtttt taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca
    79741 tgctcagagc gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa
    79801 agaatcgata ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc
    79861 tacaaaagcc actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag
    79921 attgttctta gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc
    79981 gttctcagaa tctaataccc accataaata gccatatctc atattttgta agtcttctaa
    80041 agcactggtt ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc
    80101 tttatacatc cctttattta ggcataatac accaattttt gccagatcac ttgccgataa
    80161 acacaaacca tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt
    80221 aggagactta tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac
    80281 atggtttggt atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag
    80341 cttacttgtc tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa
    80401 ttcgccggtt ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc
    80461 acttgaacag acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag
    80521 caagtgcttg attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg
    80581 aaaataatct aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc
    80641 aatcagcaaa gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt
    80701 ataaccattc cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca
    80761 tatatttggt tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta
    80821 cctcactttc ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt
    80881 cacgtaatac aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa
    80941 aatataaagg ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa
    81001 tttaccaaca ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt
    81061 gattagaggt gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat
    81121 aaactaggtt tttaaaggtg actatggtaa gataatagag atattatttt gtgattagga
    81181 gtgtatatat gaataaaaat ggagaatgga gtgctgcacg acggatattc attatattaa
    81241 tggatgttat tgtatacaat gcagcaatat atagtagttt cttactgaag tttcgtggag
    81301 agatcccatt acgaaattta gaaactttcc aacactcagc aattattatt tcagtgattt
    81361 ttattgcgtt gaacattttg ttgggtgcat atgtttttta taatcgaatg atcagtgaca
    81421 tcgtgtttgt cacagtcatc ggtcaggtat tgatgacact agggatcatg gtagtgactt
    81481 ttgcgggccg ctggtttgct tttccacgtc ttgttatctt actttcattt gttctcggta
    81541 cgcttttctt aagtatctat cgtattctcg tatataaact ttatttacgc gtaagtcgag
    81601 ataaaaaggt cgtcattatt gggttagaaa aggatgtcgc accagcaatc caaaattttg
    81661 gttcaaagaa aaacaacaaa cataaagtcg aagcagtcat tcttgataac tattatgcaa
    81721 acatcaaaaa tatgattaaa gacatcgata ttgtctatct tgcttctcat atcgaagaaa
    81781 ctgaaaaaat caaaatctat caattagtta ctaaaaatga aaaaaaattg tttttgaata
    81841 caacatttga aaacttgaca ttgatcaatc caaatattat gaatttcgaa gatgaaagta
    81901 ttattgaagc atctggattt agaatccaac ctgaatatga actattcaaa cgtttgtttg
    81961 atatcatcgt ttcacttgtt ttattagtta ttgcttcacc atttatgtta ttcacagcaa
    82021 ttttagtcaa agtgacatca ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa
    82081 atcaaaagga attttcaatc tataaattcc gctcgatgac cgccacagcc gaagcaaaat
    82141 ctggaccagt tttagctaag agcaatgatg cacgagtaac gcctgtaggt aaatttattc
    82201 gagctgtccg ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga
    82261 tcgtaggacc acgtcctgaa cgttctttct tcgtcgatca gttcaatgca gaaaaccctt
    82321 attactactt acggcataat gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa
    82381 aatatgtctc tgattacaac agtaaattac gttttgattt actttatatc aaaaaatatt
    82441 cattgatgct tgatttaaag atcttgctgc aaaccatcaa gatcctattt gataaaatgt
    82501 cctctcaagg acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt gcttttccga
    82561 atgaaaaaat ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa
    82621 tcgtaccagt atacaatgca gaagcaacta ttggtcgaac gattgatatt ctaaaagaat
    82681 tggattatga agagtacgaa gtcgttttag taaatgatgg atcaacagat caaacacagg
    82741 aaatcattca agaactgatt gaaaatgatg aacgttttga attgattact acgaccaatc
    82801 aagggcccgg attagcaaga aacgaaggaa taagacagtc aaaaggagaa tatctcttat
    82861 tcttcgatgc agatgacacg ccagtaaaaa cgatcttgtc agattatgat catttgctag
    82921 aaaagagtcc aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca atggacgagg
    82981 gaagaatcgt ttccgaaaaa gaaaacttag ttacagaatg tcactatcta actcacgaag
    83041 agtttataaa agacatgtat cagttaatga atgaacagtt gatgtatgtt gtatggaaca
    83101 aatgctatcg tagcgatatt ctgaaaaata accaaattct atttaaaact tataatagtt
    83161 gtgaggatcg aatatttaac ttacattatt atgaatactg tcatcaagtg atcatgaacc
    83221 caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac
    83281 ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta acagacgata
    83341 ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta
    83401 tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga
    83461 gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa
    83521 taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa
    83581 gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa
    83641 tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag
    83701 cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt
    83761 ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt gaaagattag
    83821 gaggccgtat ttttgttgta cctaatccta agaaaaatat ttttaaatat ttaatagaag
    83881 tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt tattttggcg
    83941 ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt gctcatagcc
    84001 atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa
    84061 ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa gctggtgaaa
    84121 gtctgtttca aaaccatccc tttgagatag ttcataatgg tattgattta gagctttata
    84181 gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca atgagtactt
    84241 ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat ttgttagaga
    84301 tttttttaga aatactaaaa atgaattctg attcttactt gattttagta ggtgaagggt
    84361 cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa agagtaaaat
    84421 ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt tttgtaatgc
    84481 cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca aaattaaaat
    84541 tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg gattttgtat
    84601 cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg tatgctaata
    84661 aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa atggagagta
    84721 tatatagtag agatcattaa aaatgttcaa gatattttag gagtgattat ttttgaaaat
    84781 atgttatttg atattggcat acaatgatat tggcaactta aagagattgg tcaatcgatt
    84841 gaacgaaaat gcagttttta ttattcatat agataaaaaa actgatatag aaccatttat
    84901 tgaagcattt aaatcatatg ataatgttga atttttatgt aataaaaacc gtgttaaaat
    84961 ttattggggt ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga gagcattaca
    85021 aaatgagaaa tatttaaaat atgtattatt atctggtgct gattatccaa tcaaagataa
    85081 tgagtatatt tataattatt ttaaaaagaa tagttcagtg gagtttattc gtggaataga
    85141 tctagatcaa attaaacata aagaatttta ttataaacat atagatgtat atcaaaaaca
    85201 tgattatcct agaattaata ggaataacac aactgcattt aaaattttta gagctataat
    85261 taatcgttgt ctgaggatga ttaaacttcc cccaaaaatt agacaccata aatttgattt
    85321 gtatcatggt tctcaatggt gggctttgtc gaaagaatgc ctaacagaat taatacaaat
    85381 gtatgaacaa aatcaacaag actacctgaa ttttaaaata ggtatgtttg ccccagatga
    85441 aaaatttttt catacacttt tttttaattc ctcgttcaaa aataaaaatg ttattggagg
    85501 accagattta ccacttgaat tgaaaaatat tgaagaaact actttacaaa cttcgaaatt
    85561 agcaaatatt catattattg atccttccat gaacaaatgg tttgatgaaa gggatataga
    85621 tcaaataaaa gaaagtgaaa aattatttgt aagaaaagtt aaatcggatt actctgataa
    85681 actattaaat agaattgata aagaaatttt gaaagttgga aatatagatg aatagaatag
    85741 tacctgtttt agagaagagt ttattaagtc gagatgcgtt tcgagtaata aatttaatca
    85801 tttgtctgct ttattttatg cctagtactt ttaatgtaac taatatcccg atgaaaatag
    85861 cttttgcttg gggtggaatt atagttttgt atgaggtttt tataaagaaa aattttttta
    85921 aaatgcgttt ttcttggcta ctgattggag caatgattag ttttatctta actataatca
    85981 ttaacagaga aaatttttta attccatcta tttataattt tggatatttg ctgattactt
    86041 tgatagtaat ttttcctgca gacttttcac aaagtgaaga ggaaaaaaag aaaaaaatgg
    86101 tgtattttaa taatgttttc attatcataa tttttttagc tgcgctaata tctatctatc
    86161 aatttatttt tttgatttct tatcatgtcc caacaggaac gccaggatta cttgcaagac
    86221 aaggttttat tgaacatcgg ttatttggtg tctatacaag tccaaatgtt ggagctatgt
    86281 ttggatatac ttctattatt ttaagtttaa ttagtgtatt aatttcaaaa ataacaaaaa
    86341 cgattcgtgt tttttatatg atgaatttta tcgtacaaat attttatatt acactttctt
    86401 cgtctagagg tgcccaggta gtaatttgtt cttttttagt attatttctt ttgttgtttg
    86461 ccaattcaaa atttagaaag agcattagag aagtactacc tgtaaaaatc aaatggatgg
    86521 taagcatatt attagttcta ttggtatatt tttcttttgg tactaattat gctaaggatt
    86581 ttttagcaac aattcctgta tcgattgata gaaaagtaga agttatagag tcaaatgaat
    86641 cagataaaac tccacttcca gaaaaagaag taatattaca gcattctagt gataactccg
    86701 aaatttcagc tggtcgtttt acgatttgga aagctgcgct taaagttata agaccaactt
    86761 tgttatttgg ttatggagag accgatttct atgggaatag cgatgcaaaa tttttgaaaa
    86821 aagtagatct tgatactaca gatgtgaatg aactaaaaag agcacatgga aacatgcata
    86881 atggatttct tcaagtactt gtatcaagtg gtataattgc ttttatatgt atctatgctt
    86941 tctatgtgtt aaatatcttt agtttactca aaacattttt tagaagtaaa gtgaattatg
    87001 cgtttcttgg tattgtttta atttttatac tatctatttt tatagatgaa atggtggagg
    87061 ctcatgtact tttcaataag cgcgatgtca tttcaattgt tttctggtat tatttgggaa
    87121 gcatttctta cttgtattta aaaaaggaga attaatatga agacagcttt cattgcttgg
    87181 actcctttac atttgattaa tatattaaat gtttatcaaa atttttaccg agaatcaaaa
    87241 ggagatttat ttatttattc tgaatttgaa aacgctagaa gatatagtga gcgtataaaa
    87301 gaattagggc tatttaatga tgttgttttt gtgaatcacc ggaaaatcgg ttcttccttt
    87361 aaacgtaaaa ttaacttgtt aactaaccat aatcaatttt tctcggatga tttatccgtt
    87421 tatgatgaaa tttttataca gggggaaaat tatttcacaa aaatactttt ctccaatgtt
    87481 tacaaaaaaa atccagatgt aaaactaaat tatatagaag atggtttggg tacttatttg
    87541 aagaagagta tattttcaac aagtaaaaac gttcaaagag tttttaaaat attaaataaa
    87601 aatagcatct tcaaaaaaaa aattgacgat tattttgtct attgtccgga attgttactt
    87661 tatgatgtaa aaaatataaa aaaaattccc aaaatagatg aaaacagtaa attgatgaaa
    87721 aacatacaat caatctttgg gatagaagaa gtttttgtcg gagataaatg tatctttttt
    87781 gatcaacctt tattatctga tggttatggc atagatgaaa cagatatttt cgctaaagta
    87841 aatagtatat tttctggaaa aaaagaaact ttaattaagc ttcatcctag agcaaggaca
    87901 aatcgttatg gtgaaaaaaa catattgaat tccagtttac cgtttgaagt cttgctactt
    87961 gaaaatgact tctcttcaac acttttgatt agtcctatat ctacaatttc tttttcacca
    88021 tctatgatgt ttgaaaagac gatcaactct attatgttgg ctaaattaat tttaaatgaa
    88081 tatacagcat tatctgaaga taaaaaagaa acattgacaa atattgttct tttctgtgaa
    88141 cattataacc gtctatcaga agaaaaaatt ttactcccag aaaactgggc agaattaaga
    88201 aagttggtag agtatgggat tatttaacac actttccaag tcaaaattag ttaagtccag
    88261 tttctggtat actataggga atttttttat taagggaatt tcattcataa ctattcctat
    88321 ttttgtaaat attactactc aagcagaata tggtttgatt aataatttca cttcatttgt
    88381 atcaattttt tcgattcttg ttgggctaag cctaaattcg gcaattaata atgcaagttt
    88441 tgactataaa gatgacataa atgaatttat gtcatcggta ctttctctag ctacactttc
    88501 gttaattgtg ttcttattaa tagggaacat atattttttg ttttcaaatg ctttttttga
    88561 tatgaaccaa cttgtattta atttgatgtt attacaaagt tttgctagtt ttgtaattag
    88621 ttatatccag tcttatttta cgattaactt taaccatttt aaatatttga ttttatcttt
    88681 tatatctact tttttgaata ttgcaatctc tgtagctttg atgcagacat tatattttga
    88741 tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga taggtttagt
    88801 aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac agtggagata
    88861 cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag ttttagcaca
    88921 attcgataga attatgatta attcttattt aggtcctgct gcggggggaa tatattccta
    88981 cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata acgcctgggt
    89041 tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa
    89101 ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt cgattgattt
    89161 agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg ttccgataac
    89221 cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat tttatgagaa
    89281 aaaaacatcg tttattgcga ttggtacagt agcaagtgct atagttaata tactattaaa
    89341 catgattttc atacctaggt taggaataat tgctgctgga tacacaaccg tatttacgta
    89401 ttttttactt tttatttttc attataatat cgctaaaaaa attacgaata aacaattatt
    89461 tgacacaaaa aaaatatgta tcataataat ttttgtatca attttttctt tgatacttca
    89521 tattttaatc aatcaaatgt tgattcgtta cgttttgaca tcattattga taattagttt
    89581 agttatatat tttttaaaga ttaagaatcg aattatagat gactataatt cataggagga
    89641 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
    89701 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
    89761 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
    89821 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
    89881 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
    89941 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
    90001 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
    90061 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
    90121 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
    90181 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
    90241 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
    90301 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
    90361 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
    90421 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
    90481 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
    90541 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
    90601 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
    90661 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
    90721 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
    90781 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
    90841 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
    90901 caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga ttttttgtct
    90961 actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa
    91021 attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat gtatgggttg
    91081 ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc tgtagattgt
    91141 ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc tagttctgaa
    91201 gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc cgttgggtac
    91261 tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg tgctaaggta
    91321 attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa agcttcttta
    91381 gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat atttttaggt
    91441 gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc aagaaaaagc
    91501 ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc tcttacaata
    91561 aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg caaaaaagtt
    91621 aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg aaaaaaatac
    91681 ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct gagaatttac
    91741 tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc cataaatatg
    91801 gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa gttcaaagtt
    91861 tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag ataaatggtt
    91921 tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg gatagaggag
    91981 aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca catattcacg
    92041 gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg aaaatagcat
    92101 ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg ggggaagatt
    92161 cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat aatagattac
    92221 ctagttttag agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt
    92281 tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa attttagctt
    92341 tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct aatagtgatg
    92401 ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat attatttata
    92461 ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa tttatgatcg
    92521 gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta ataaatttag
    92581 gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa
    92641 atgaaattga aactgctttg aataaaatac aagatcctca gtttataaaa agcttgaaaa
    92701 atgttaagaa catctacggt gacggcaaca gttctagaag aataacaaaa acattgaatc
    92761 aaatattgaa tgatggggaa gatattaagt ggattcaaaa acaaatagct tattaaaata
    92821 tactagtgat ccatacatta ttattggggc tggcggacac gcaaaagtca ttattgaaac
    92881 cttaagatta gaaaataagt taatatatgg attttttgat gataagcaaa ataaaatgca
    92941 agatctgaaa tatcttggga aaataaacga attgaatagc tgggcggata gcaaaaaaaa
    93001 aatctataaa tttattgtag ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa
    93061 attgaatatg ccaagtgagc aatatggaat agctgttcat ccaagtgcta tagtggcaaa
    93121 agaaatttca attggttatg gaactgttat ttttgcgaat gctgtaatta acccagatgc
    93181 tgtaattgga gagcatgcta taataaacac aggtagcatt gttgagcatg attgtagaat
    93241 taataactat gttcatttat ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt
    93301 aggaactcaa ataggaattg gttctcaatg tatccagatg aagaaaattg gatcttggtg
    93361 tatgataggc gcagggagca ctattgttaa ggatattccc tcacattcct tagcttatgg
    93421 caatccagct aaaattaaga aagaaggaat taattttgaa ttctagaatt ttattatcaa
    93481 gtcctcatat gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa gataattgga
    93541 tagcgccatt aggtgaaaat gtggataaat ttgaagaaag tattacaaaa tatactggtt
    93601 ctaagggcgg agcttctgcg ttaagttcag gtacagctgc catacatctt gccttaagac
    93661 tgcttaatgt tggaaaaaac gatattgtct ttgctccttc atttactttt atagctacag
    93721 taaatcctat tttatatcaa ggggctgtac cagtgttcat tgactcagaa cctaatacat
    93781 ggaatatgag ccctgttgca ctagagagcg cattagaaga agctgaaaga aacaaccaat
    93841 tacctaaggc aatcatcgta gtgaatttat atggtcaaat ggctgattat gaacgaattt
    93901 tagctattgc aaaccaattt aatgttccag tgatagaaga tgcagcggaa tcgttaggtt
    93961 ctacttataa gggaatttcg tcaggcttat ttgggacaat tgggatctat tcatttaacg
    94021 gtaataagat catcacaacc tcaggtggag gagctctagt gtctagtgat aaaaaatatg
    94081 ttgagcattc aagattttta gcaacacaag caagagatca agcaccgtat tatcaacata
    94141 gcgaaatagg ttataattac cgtctaagca atatttctgc cggtatcgga cgtggacaaa
    94201 tggaagtatt gaatgataga gtagctaaac gaagagaaat tttcaatcga tataaggaag
    94261 ctttttctgg aattgataga attgaaatga tgccagaagt ggatggcatg gtgccaaatc
    94321 gttggttgtc tactttaacg atcaatgatt ataattctat tgatcaagta gttaacgtaa
    94381 taaataaact aaatgaaaaa aacattgaaa caagagcttt gtggaaacca ctacatttgc
    94441 aaccagtatt tcaaggggta aaaatgtatt accatgataa taatcttagt gtgtcagaaa
    94501 agttatttaa tagcggctta tgcttacctt ctggaagtaa tatgacagat agtgaacaag
    94561 aatttgttat tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt atgaacatag
    94621 gaattattgg tttaggttcc atggggaaaa gaagacttag actcattcaa gaacacttta
    94681 atgatatttt actatgtggt gttgatacta atgaggcgag acgaaaggaa gttgaaaagg
    94741 aatttggaat accaacttat aatagtctaa cagaggcaaa aaatttgttt gaaataaatt
    94801 cagtttttgt ttgtacatcc cctataaccc atgaatttat tataaaagag gctttaaatt
    94861 tagattgtaa tatctttacg gaaatcaatt tattaaatga atattatgat gaaataatcg
    94921 aagtagctaa aaagaaagat agattacttt atctatcttc tacatttttg aagagaaaag
    94981 aaattcaata tattaagtct aaagtgacgc ataataaaaa aatttcttat caataccatg
    95041 taggacagta tttacccgac tggcatcctt gggaagatta tcgcaatttt tttgtttcaa
    95101 acaaaaaaac taatgggtgt agagaaatat tcgctattga attaccttgg attatagata
    95161 catttgggaa agttaaatcg tttactgtaa tgacccagaa attatccaat cttgatattg
    95221 attattcgga cagtttcact cttattattg agcatgaaac tggtatagtt ggaactttaa
    95281 gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac tataattggt gaaaagattt
    95341 taatagaatg ggaaggtaac ccggattctc tattttcatg gaatgaagaa acttctagta
    95401 tgacagatat tcaattgtac gatgaggtta tgcatgataa taattattca catactatta
    95461 ttgaagatgc atatttagag gagattaaag aatttttcac gcttttggaa aagaaaatgg
    95521 aaccccaata tacttttgaa aaagataagt atattattaa cttaattaac gacattgagg
    95581 taaattaatg atgaaagtaa ttttttttgg gttagggtca attggaaaga gacatctaaa
    95641 aaatctaata agctatggaa aaaagaattc tattaatttt gatataacag cttatagtag
    95701 cagtggaaaa aaagagtttg aagaaataac gtatattcaa aatagagacg atttggatac
    95761 acattatgat atagctttta ttacaaatcc gacttttaag catctggaaa cactaaatgt
    95821 aattgttgaa aaagttgatt acatattttt agaaaaacct gctttttctg agagtattga
    95881 tcttactcaa tataattatg attacaaaaa tatttatgtt gctgctccac tgaggtataa
    95941 aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc
    96001 tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa ataattattc
    96061 tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat tggattatat
    96121 tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat attcgaatct
    96181 tgaaattaac agtaatgatt tagcaacata tatattgaga tacgatgata aaattataga
    96241 aattcattta gattattttg gaagagttac tacaagaaaa tttcaattgt ttactttaga
    96301 tgaatgtatc aatgttgact tattaaataa taaaataagt tttgaagaaa acattgttaa
    96361 agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt tctttgaaaa
    96421 cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag tattgaaaat
    96481 agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg ggtctaaggg
    96541 gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt tcgctttagc
    96601 aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt gtttgaatac
    96661 ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga aaattgtaga
    96721 acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac gtgattcatt
    96781 cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag atttgacttc
    96841 acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa aaaatttaga
    96901 taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta atatggtaaa
    96961 aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta ggcaacagac
    97021 acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa
    97081 aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt
    97141 agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga
    97201 agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct
    97261 gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat catttaggag
    97321 aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata
    97381 tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga
    97441 tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag
    97501 attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt
    97561 tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg
    97621 ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga
    97681 cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat
    97741 atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga
    97801 attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag
    97861 gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata
    97921 tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac
    97981 aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg
    98041 aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg
    98101 actctctagc agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt
    98161 taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt
    98221 agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg
    98281 ggatagaa
//
LOCUS       DACECS010000002        89502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.002, whole genome shotgun sequence.
ACCESSION   DACECS010000002 DACECS010000000
VERSION     DACECS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.002"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..538)
                     /locus_tag="IWO31_000099"
     CDS             complement(17..538)
                     /locus_tag="IWO31_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0300555.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="IWO31_000100"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="IWO31_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0300556.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="IWO31_000101"
     CDS             4618..5505
                     /locus_tag="IWO31_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0300557.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="IWO31_000102"
     CDS             5502..6671
                     /locus_tag="IWO31_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300558.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="IWO31_000103"
     CDS             6695..8050
                     /locus_tag="IWO31_000103"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0300559.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IWO31_000104"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IWO31_000104"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0300560.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="IWO31_000105"
     CDS             10336..10626
                     /locus_tag="IWO31_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300561.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="IWO31_000106"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="IWO31_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0300562.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="IWO31_000107"
     CDS             11667..12326
                     /locus_tag="IWO31_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0300563.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="IWO31_000108"
     CDS             12483..13415
                     /locus_tag="IWO31_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300564.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="IWO31_000109"
     CDS             13569..14858
                     /locus_tag="IWO31_000109"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0300565.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="IWO31_000110"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="IWO31_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0300566.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="IWO31_000111"
     CDS             16438..17025
                     /locus_tag="IWO31_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0300567.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            17390..18718
                     /locus_tag="IWO31_000112"
     CDS             17390..18718
                     /locus_tag="IWO31_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300568.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18729..18938
                     /locus_tag="IWO31_000113"
     CDS             18729..18938
                     /locus_tag="IWO31_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300569.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            19097..19849
                     /locus_tag="IWO31_000114"
     CDS             19097..19849
                     /locus_tag="IWO31_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0300570.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            19846..20760
                     /gene="pfkB"
                     /locus_tag="IWO31_000115"
     CDS             19846..20760
                     /gene="pfkB"
                     /locus_tag="IWO31_000115"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0300571.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            20778..22709
                     /locus_tag="IWO31_000116"
     CDS             20778..22709
                     /locus_tag="IWO31_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0300572.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22820..24379)
                     /locus_tag="IWO31_000117"
     CDS             complement(22820..24379)
                     /locus_tag="IWO31_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0300573.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            24534..26768
                     /gene="pcrA"
                     /locus_tag="IWO31_000118"
     CDS             24534..26768
                     /gene="pcrA"
                     /locus_tag="IWO31_000118"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0300574.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            26785..28821
                     /gene="ligA"
                     /locus_tag="IWO31_000119"
     CDS             26785..28821
                     /gene="ligA"
                     /locus_tag="IWO31_000119"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0300575.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            28927..29232
                     /gene="gatC"
                     /locus_tag="IWO31_000120"
     CDS             28927..29232
                     /gene="gatC"
                     /locus_tag="IWO31_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0300576.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            29232..30704
                     /gene="gatA"
                     /locus_tag="IWO31_000121"
     CDS             29232..30704
                     /gene="gatA"
                     /locus_tag="IWO31_000121"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0300577.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            30701..32131
                     /gene="gatB"
                     /locus_tag="IWO31_000122"
     CDS             30701..32131
                     /gene="gatB"
                     /locus_tag="IWO31_000122"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0300578.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            32141..33196
                     /locus_tag="IWO31_000123"
     CDS             32141..33196
                     /locus_tag="IWO31_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0300579.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            33307..34680
                     /gene="rlmD"
                     /locus_tag="IWO31_000124"
     CDS             33307..34680
                     /gene="rlmD"
                     /locus_tag="IWO31_000124"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0300580.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            34683..34811
                     /locus_tag="IWO31_000125"
     CDS             34683..34811
                     /locus_tag="IWO31_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0300581.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            35251..35439
                     /locus_tag="IWO31_000126"
     CDS             35251..35439
                     /locus_tag="IWO31_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0300582.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            35517..36530
                     /locus_tag="IWO31_000127"
     CDS             35517..36530
                     /locus_tag="IWO31_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0300583.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(36597..37631)
                     /locus_tag="IWO31_000128"
     CDS             complement(36597..37631)
                     /locus_tag="IWO31_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0300584.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            37746..39971
                     /locus_tag="IWO31_000129"
     CDS             37746..39971
                     /locus_tag="IWO31_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0300585.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            39989..41872
                     /locus_tag="IWO31_000130"
     CDS             39989..41872
                     /locus_tag="IWO31_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAQ0300586.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKRSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            41853..42827
                     /locus_tag="IWO31_000131"
     CDS             41853..42827
                     /locus_tag="IWO31_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0300587.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            43486..44508
                     /gene="aroF"
                     /locus_tag="IWO31_000132"
     CDS             43486..44508
                     /gene="aroF"
                     /locus_tag="IWO31_000132"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0300588.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            44541..45605
                     /gene="aroB"
                     /locus_tag="IWO31_000133"
     CDS             44541..45605
                     /gene="aroB"
                     /locus_tag="IWO31_000133"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0300589.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            45607..46773
                     /gene="aroC"
                     /locus_tag="IWO31_000134"
     CDS             45607..46773
                     /gene="aroC"
                     /locus_tag="IWO31_000134"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0300590.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            46790..47881
                     /locus_tag="IWO31_000135"
     CDS             46790..47881
                     /locus_tag="IWO31_000135"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0300591.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            47894..49189
                     /gene="aroA"
                     /locus_tag="IWO31_000136"
     CDS             47894..49189
                     /gene="aroA"
                     /locus_tag="IWO31_000136"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0300592.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            49182..49691
                     /locus_tag="IWO31_000137"
     CDS             49182..49691
                     /locus_tag="IWO31_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0300593.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            49688..50524
                     /gene="pheA"
                     /locus_tag="IWO31_000138"
     CDS             49688..50524
                     /gene="pheA"
                     /locus_tag="IWO31_000138"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0300594.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(50587..52323)
                     /locus_tag="IWO31_000139"
     CDS             complement(50587..52323)
                     /locus_tag="IWO31_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0300595.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(52320..53249)
                     /locus_tag="IWO31_000140"
     CDS             complement(52320..53249)
                     /locus_tag="IWO31_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0300596.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(53249..53827)
                     /locus_tag="IWO31_000141"
     CDS             complement(53249..53827)
                     /locus_tag="IWO31_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0300597.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            54100..55386
                     /locus_tag="IWO31_000142"
     CDS             54100..55386
                     /locus_tag="IWO31_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0300598.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            55523..56392
                     /locus_tag="IWO31_000143"
     CDS             55523..56392
                     /locus_tag="IWO31_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0300599.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            56389..57276
                     /locus_tag="IWO31_000144"
     CDS             56389..57276
                     /locus_tag="IWO31_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0300600.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            57443..58126
                     /locus_tag="IWO31_000145"
     CDS             57443..58126
                     /locus_tag="IWO31_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0300601.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            58123..58896
                     /locus_tag="IWO31_000146"
     CDS             58123..58896
                     /locus_tag="IWO31_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0300602.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            58893..59291
                     /locus_tag="IWO31_000147"
     CDS             58893..59291
                     /locus_tag="IWO31_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0300603.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            59476..60148
                     /locus_tag="IWO31_000148"
                     /pseudo
     CDS             59476..60148
                     /locus_tag="IWO31_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            60207..60647
                     /locus_tag="IWO31_000149"
     CDS             60207..60647
                     /locus_tag="IWO31_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300604.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            60659..61327
                     /locus_tag="IWO31_000150"
     CDS             60659..61327
                     /locus_tag="IWO31_000150"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0300605.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            61391..62014
                     /locus_tag="IWO31_000151"
     CDS             61391..62014
                     /locus_tag="IWO31_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0300606.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            62070..62456
                     /locus_tag="IWO31_000152"
     CDS             62070..62456
                     /locus_tag="IWO31_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0300607.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            62669..64060
                     /locus_tag="IWO31_000153"
     CDS             62669..64060
                     /locus_tag="IWO31_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0300608.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            64407..65054
                     /locus_tag="IWO31_000154"
     CDS             64407..65054
                     /locus_tag="IWO31_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0300609.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            65447..68092
                     /locus_tag="IWO31_000155"
     CDS             65447..68092
                     /locus_tag="IWO31_000155"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0300610.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            68402..69715
                     /locus_tag="IWO31_000156"
     CDS             68402..69715
                     /locus_tag="IWO31_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0300611.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            69705..70358
                     /locus_tag="IWO31_000157"
     CDS             69705..70358
                     /locus_tag="IWO31_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0300612.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            70413..70964
                     /gene="radC"
                     /locus_tag="IWO31_000158"
                     /pseudo
     CDS             70413..70964
                     /gene="radC"
                     /locus_tag="IWO31_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            71095..72180
                     /locus_tag="IWO31_000159"
     CDS             71095..72180
                     /locus_tag="IWO31_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0300613.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            72177..74069
                     /locus_tag="IWO31_000160"
     CDS             72177..74069
                     /locus_tag="IWO31_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0300614.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            74076..74453
                     /locus_tag="IWO31_000161"
     CDS             74076..74453
                     /locus_tag="IWO31_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0300615.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            74607..75389
                     /locus_tag="IWO31_000162"
     CDS             74607..75389
                     /locus_tag="IWO31_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAQ0300616.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(75515..76246)
                     /gene="erm(A)"
                     /locus_tag="IWO31_000163"
     CDS             complement(75515..76246)
                     /gene="erm(A)"
                     /locus_tag="IWO31_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ0300617.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(76304..76391)
                     /locus_tag="IWO31_000164"
                     /pseudo
     CDS             complement(76304..76391)
                     /locus_tag="IWO31_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            76755..77417
                     /locus_tag="IWO31_000165"
     CDS             76755..77417
                     /locus_tag="IWO31_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0300618.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            77652..77795
                     /locus_tag="IWO31_000166"
                     /pseudo
     CDS             77652..77795
                     /locus_tag="IWO31_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            77945..78145
                     /locus_tag="IWO31_000167"
     CDS             77945..78145
                     /locus_tag="IWO31_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0300619.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(78399..80078)
                     /locus_tag="IWO31_000168"
     CDS             complement(78399..80078)
                     /locus_tag="IWO31_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0300620.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(80080..80292)
                     /locus_tag="IWO31_000169"
     CDS             complement(80080..80292)
                     /locus_tag="IWO31_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0300621.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            80685..82415
                     /gene="efrA"
                     /locus_tag="IWO31_000170"
     CDS             80685..82415
                     /gene="efrA"
                     /locus_tag="IWO31_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0300622.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            82412..84172
                     /gene="efrB"
                     /locus_tag="IWO31_000171"
     CDS             82412..84172
                     /gene="efrB"
                     /locus_tag="IWO31_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0300623.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            84267..84464
                     /locus_tag="IWO31_000172"
     CDS             84267..84464
                     /locus_tag="IWO31_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300624.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            84617..85102
                     /locus_tag="IWO31_000173"
     CDS             84617..85102
                     /locus_tag="IWO31_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0300625.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(85206..85799)
                     /gene="clpP"
                     /locus_tag="IWO31_000174"
     CDS             complement(85206..85799)
                     /gene="clpP"
                     /locus_tag="IWO31_000174"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0300626.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            85979..86506
                     /locus_tag="IWO31_000175"
     CDS             85979..86506
                     /locus_tag="IWO31_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0300627.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            86597..87334
                     /locus_tag="IWO31_000176"
     CDS             86597..87334
                     /locus_tag="IWO31_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0300628.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            87338..88255
                     /gene="miaA"
                     /locus_tag="IWO31_000177"
     CDS             87338..88255
                     /gene="miaA"
                     /locus_tag="IWO31_000177"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0300629.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            88257..89486
                     /gene="hflX"
                     /locus_tag="IWO31_000178"
     CDS             88257..89486
                     /gene="hflX"
                     /locus_tag="IWO31_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0300630.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        29402 a        15587 c        19238 g        25275 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tctagatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    18841 ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct gcttctgttt
    18901 atatgaaaaa aacaagaagc gaaaataaaa aacgataaaa cggcttgatg atagacaaaa
    18961 aaataaaaaa ccagtctttc tgactggttt tttatttttt tttgactgat ttataatttt
    19021 tatgaacgtt tttgactgat ttatattgct agtagaaaaa aaatatgcta ttatttgtat
    19081 tgaggtgatc gtgaaaatgc ttacagagga acgcagacaa aaaatcctag acttgcttga
    19141 acagcagaaa atcgtgaaat cacaagatct tgtaaacctt ttgaatgctt ctgaatcaac
    19201 gataagacgt gatttacaag agctggagaa tagtgggttt ctagaaagag ttcatggcgg
    19261 agctaagaaa gagcaattat tgaattttga gcagaatatg agtgaaaaat cactcaaaaa
    19321 cgttcacgaa aaacagcaaa tcgccaaatc ggcagctgaa accattaatg acgaagatgt
    19381 catttattta gacgctggct ctactacgtt agaaatgatt ccttttctgc aaggaaaaca
    19441 gatcacagta gtgacaaatt ccgttcaaca tgcggcaaat ttagttgatt tggagcttgc
    19501 taccattatt ttaggtggaa cgataaagct ggcaactaat gctgttttag gatctaatgc
    19561 gatagaacaa ctaaaaaatt ttcattttaa taaagcattt atgggaatga acggtgccca
    19621 tcttgaacaa ggattcacga ctccggatcc agaagaggca gctgttaaac gtttagccat
    19681 gaaaaattcc gaagagacat ttgtattgat tgatcatacc aaattcaacc agttgtcctt
    19741 cacctctgtt gcacctttgg aagcagccac gatcataacc gagcgttgtc cattagaatt
    19801 cgttaatgaa ttccgtgaaa agacaacaat taaggaggca atacaatgat ttacacagta
    19861 acacttaatc catcaattga ttatatcgta cgcgtagatg ggttaaaact aggcgatttg
    19921 aaccgaatga aggaagactt caagttacct ggaggaaaag ggatcaatgt ttcccgtatt
    19981 ttgaaacgga tcgattctca atctacagca ctagggtttt taggagggtt cactggcgaa
    20041 tttatttcga agtggcttga aaaagaacaa atccagtctt tgttcacaac cgtaaaagaa
    20101 gatacaagaa tcaatatcaa actaaaatca gacactgaaa ctgagatcaa tggcttaggc
    20161 ccatctattt cagatgaaga aatcgatgag ttgaagcaag cattaggaac agtaggacaa
    20221 ggagatatcg tcgtattgtc aggaagcgcg ccagctagcc tgcgaaaagg attttatcaa
    20281 gaactgattg atatcattcg agaaaaaggt gcagatttcg tgattgatac aacaggtgat
    20341 gacctaaaaa atgcacttgt taagaaaccg ttgttgatca aaccaaacaa tcacgaatta
    20401 gctgagcttt acaacacaac cttccaatct gtagaagaaa tcattccgta tgggaaaaaa
    20461 ttactagctg aaggggcaca gaatgtgctg atctcaatgg ctggagacgg tgctttgctc
    20521 ttcacggaac aaggaactta taaaacgaat gtattagttc gcccattgaa aaattcagta
    20581 ggtgcagggg attcaatgat tgctggcttt attggggctt acagccgtac gaaagatcca
    20641 atcgaagcct ttaaatgggg agttgcttgc ggaagcgcaa cagctttttc tgatgacttg
    20701 gcagctcgcg aattgattga tgagttgctg ccagaagttg aaatcacaaa aattgattag
    20761 tttcaaggag gataatcatg aatatcaagg atttaatggt caaagaagcg atgatcatgg
    20821 atttgcaagc gactgataaa aaaggcgcaa tcgatgaaat ggtccaaaaa atgtttgatg
    20881 ccggccggat ttccgatatc gaaacatata aagaaggaat tctagcccgc gaagcacaaa
    20941 cttctacagg tcttggtgat ggaatcgcca tgcctcacgc gaaaaacagt gcagttaaag
    21001 aagcaacagt tttattcgca aaaagccaaa aaggagtaga ttatgaagcc ttagacggac
    21061 agccaactta tctgtttttc atgatcgcag ctccagaagg cgcaaacgac acacatttgc
    21121 aagcattagc agcactatct cgattgctga ttgatgcaga tttcgtagga aaattaaaag
    21181 aagcaaatac accagaagaa gtccaagact tgttccaaac agctgaagag caaaaagaag
    21241 cagaagaaaa agcagaacaa gaaacacaag ctgcagaagc ttctgctcat ccagaaagaa
    21301 aattcgttgt tgccgttaca gcttgtccaa cagggatcgc tcacacttac atggcagaag
    21361 atgctttgaa gaaaaaagcg aaagaaatgg gtgtggagat caaagtcgaa acgaacggtt
    21421 cagaaggaat caaaaaccga ttgactgctg aagacatcgc ccgtgcagaa ggtgtaatcg
    21481 ttgcagcaga caaaaaagta gagatgaatc gctttgacgg aaaagaactg atcaatcgtc
    21541 ctgtcagtga tggtatccgt aaaccagaag agttgatcaa tctagccctt agtggtactg
    21601 caccagtatt ccatggtgat ggaaaagaaa gcagttcaga agaaggaaat gcggatggta
    21661 ctatcggaca aagaatttac aaagatttga tgaacggcgt atcacacatg ttacctttcg
    21721 ttattggtgg aggaatcgct attgcgcttt ctttcatgat cgaccaattt attggcgtgc
    21781 cacatgatca attatcaaat ctagggaatt acaatcaagc tgctagctgg ttcaaccaaa
    21841 tcggacaagc tgcatttggt ttcatgctgc cggtattagc aggatttatt gcttcaagta
    21901 tcggtgaccg ccctggtttg atcgttggat ttgctgctgg tgcattagct aatacaggcg
    21961 gtgcaggatt cttaggtgca ctgattggtg gattcttagc tggttatgtc atcgtatttt
    22021 tacgtaaatt attcaagaac ttaccaaaat cattggatgg aattaaaacg atcctctttt
    22081 atccagtgtt tggcttactg attactggtt tcttgatgtt gctggtcaat gtccctatga
    22141 aagcaatcaa tgatgcattg aacggtttct tgacaagtat gagtggttcg aatgctgctt
    22201 tacttggtgc attattagct ggaatgatgg cggcagattt aggcggtcca atcaacaaag
    22261 ccgcttatgt gtttggtaca gcaactttag ctacgactgt tgcaactggc ggaagtgtcg
    22321 tgatggcttc tgttatggct ggaggaatgg tacctccatt ggcaattttc gttgctacac
    22381 gtttgtttaa gaataaattc agcaaaacag atcaagatgc tggtttgacg aacatcgtga
    22441 tgggactatc gtttgtcacg gaaggatcga ttccatttgc agcagctgac ccgattcgtg
    22501 ccattccaag ttttattatt ggttctgctt taactggcgg attagtcgga gctttcggaa
    22561 tcaaattact ggctcctcac ggcgggattt tcgttgtatt cttactgagt cacccggtca
    22621 tgtatttggt gtttattgct atcggagcag tcgtatctgg tgtgatttat ggtttattga
    22681 gaaaagctcc tgcagtagca gaagcataga aataaaaaag aagaggctgg gttttcccag
    22741 cctcttcttt ttttatagaa tcatatagaa tcaagtagtg tagatgaata gttgttgtaa
    22801 aatagttttt ccatgttcgt taaaatgatt gcttcaaatt tgtgatagaa gatttagcct
    22861 cgatttcctg cttgattctt ggcaagtttg cttcatcagt caagataacc aaaatatctc
    22921 ctacattcat gatcgtatca ccgtgagtca agatttcctg atttcctctg cgtatagaga
    22981 ttagcagcat atttttaggc caattgaagt ctcgtaccat taatccgtct aaagaactct
    23041 ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt tagtgaggcg gtctgagtat
    23101 ttccaagtag acgttccaat aatgattcgt aaattggatt accgccgaga aggtcattga
    23161 cgatataagc aacaagagca gccaatccta aaggcatcag atgattaaga gagccaacca
    23221 tttctgtcac taaaataatt gctgtcagcg gtgcttttcc aatcgctgtg aaataaccag
    23281 ccatggcaat aacgacaaaa tccttgatgt agattggatc catcccgaaa tcaaccataa
    23341 tgtttccata aaagctaccg attaaagccc ccaatgtcaa tatcggaagg aaaatccccc
    23401 ctggaagacc cccaccgtaa gaaatcatgg aaaaaacgaa tcgaagaaca aacaaaccga
    23461 taatcataat gattggcaag gaagcatgtc caaaagctaa aacgatttga ttgcctccgc
    23521 ctagagtact aggccataaa taaccaattg gcaaaatcag caagaaagga acgaacccgt
    23581 gaaaataagc tggtaaaggc aatcggttgt aaaattttgg caaagccagc aagacattct
    23641 gataaataaa acctaagatg cctagaagta ctcctaagat cactaaggaa ccataatatt
    23701 tcaacggcaa agaaggaagg tcacctagat ataaaacagg tctcaagccg aaaaagtaaa
    23761 gagagacgaa gttagcagtg attgctgaag caaatgatgt cagccaaaca agtggagaaa
    23821 aggagtgatg gatttcttct aaaacaaaga gtaatcctgc gataggagca ttgaatgctg
    23881 ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt ttcttctgaa gatggactcc
    23941 gaaacaattg tccagctcct tgtccaatgg atgcgccaag ctgtatagaa ggtccttctc
    24001 tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc aatgaacttt ttccataaaa
    24061 cagagaacca gttcattgat aattccccgc gaagctgtcc ctctacttgt ggaatcccgc
    24121 ttcccttgat atccggttca tttttgacca gataacccac gaaaaatgct gcaaggatgc
    24181 agcaaatagc taggccaatt aagtaaatcg gctgttcccg actcatttca tagatacttc
    24241 gaactaaatc gcttagcttt tcgatagata atcgaaacag gctaacgacg attcctgtca
    24301 gcagtccaac aaatattcct tttagaataa aatatatctg agtcttatct aatttcttga
    24361 tttttccttt tttttccatt caaacgcctc gctttctttt aatttagcgc gaaatcaacg
    24421 aattgacaag gaaaaatcca taaaaagaaa caaatgattt tgaactagct agtttaatga
    24481 aagccaagaa aatgctataa ttaacaagat tgataaaaga aaggatgttg ttaatgtcat
    24541 ccaaagctga attattaaac gggatgaacc cccgtcagaa agaagctgtt cttcacactg
    24601 acggaccatt actgctaatg gcaggtgcag gaagtggaaa gacgagagtg ctgacacacc
    24661 gcattgccta tttgattgag gaaaaagaag tcaatccttg gaacatccta gcgattactt
    24721 ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa tgcgattctc gcatctggag
    24781 gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt gcgtatttta agaagagatg
    24841 ttgattttat cgggtataac cggaacttta caatcatcga ttcttctgaa cagctgacct
    24901 tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa aaaatatgat cctcgaagta
    24961 tattaggaac aatctctcaa gcaaaaaaca gcctgcagac ccctcaggat tttgcaaaaa
    25021 tgcaaggcag ttattacgaa gagattgcgg caaaatgtta tgctgcttac caaaaagagc
    25081 ttcaatataa tcaatgtatg gactttgacg acctgatcat gaatacgatt cgattatttg
    25141 aagaacatcc tgattcactc acgtattatc aaaacaaatt ccattatatt catgtagatg
    25201 agtaccaaga tacaaatcac gcacagtata cgctagtcaa tttattggca ggtagattta
    25261 ggaacctgtg cgtcgtggga gatgcagatc aaagtattta cggctggcgc ggtgcagata
    25321 tgcagaacat cttggatttt gaaaaagatt atccagatgc tgcagtaatt ttgctagaac
    25381 aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa ccaagtcatt gaaaacaata
    25441 gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga aggaaacaag atcacttact
    25501 accgtgcaga taatgaacga gacgaaaccc gttttatcgt agaccggatg caagaagaga
    25561 ttcgaagcaa tcatcggaat tacggcgact ttgccatctt gtaccggacg aacgcacaat
    25621 ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc ttataaaatg gttggagggc
    25681 acaagttcta cgatcgtaaa gaaatcaaag atattttagc gtatttgaat gtgctggtaa
    25741 atcctcaaga ttcaatcagt tttgagcgta tcgttaattc gcccaaacga ggcatcggac
    25801 caggatctat cgaaaaacta cgcagttttg cttctcttca tgaatggcca ctgcttgaag
    25861 cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc aggacagcaa ttaggtgcat
    25921 ttggagagat gatccaagaa gtcactcaga tgatcccata cttgacagtg acagaattga
    25981 cgaaagaggt tttggatcgt agcggatatc tagaagactt aaaaatccaa aatacattag
    26041 aagcacaagc acgcatcgaa aacttggagg aatttttaac ggtcacacaa gaattcgaca
    26101 aacaattcga acagcaaaat gaagaagacg ctgatgcacc agaagaaaaa ctaacggtct
    26161 tcttaaatga tctggcactt gtttccgaca ttgacaattt agaagaagat gcttcccaag
    26221 tgacattgat gactttgcat gcagccaaag gattagaatt tccagttgta ttcctgattg
    26281 gtctagaaga gggggttttt cctttatctc gagcattgat ggaagaaagc gaactggagg
    26341 aagaacgccg tctcgcatat gtggggatca cgcgagcaga agaagcgttg tatttaacaa
    26401 atgctttctc cagaacatta tatggacgga cgcaatataa ccgaccttct cgttttgtag
    26461 aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg tcctacgcct aaaaaaacgc
    26521 ctgtattcgc taaaaaaaca gcatatagct ataaacagcc agagacagcg gttgttccct
    26581 ctaaaagtgc tactggaggg gaaaataaca gctggaaacc tggagataaa gtcaaacaca
    26641 aaaaatgggg tctaggaaca gtcgtccgag taagcgggac gtcgaaggat ctagaactag
    26701 atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc tgcctttgcg ccaattgaaa
    26761 aagcttagta gtggagggta aatgatggaa catgaaatga cgattgacga agctgctaaa
    26821 cgagcagaag aacttcgtac aagactgaac caatggtctc gcgaatacta cgtagaagat
    26881 aaaccaacag tggaagacta tgtttatgat aaagaatacg cagaattagt agcaatcgag
    26941 gaacaatatc ctgatttgat cacgtctgat tctcccactc aacgagtagg cggaaaagta
    27001 ttagaaggtt ttgaaaaagt cactcatgat attccgcttt acagtttgaa cgacgttttc
    27061 agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa aagcagtggg acgtgtagtt
    27121 gactattgct gtgaactaaa aatagatgga ctttccgttt ctttgcgtta tgaagatgga
    27181 aatttcgtga gaggtgcgac tcgcggggat ggaactgtag gagaaaacat cactgaaaac
    27241 ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac caatgaacat cgaagtaaga
    27301 ggcgaatgtt tcatgccaaa acggtcattt gtccaattga accaagacag agaagcagag
    27361 ggaaaagata tctttgcgaa tcctcggaac gccgcagctg gttctttgcg tcagttagat
    27421 tctaaaatca cagcgaaaag aaatctggac acttttttat acacagtagc tgatttcggt
    27481 cctatgcaag caaaaaccca gtatgatgcg ctagaagagc tagaaaaaat tggattccac
    27541 acgaatcggg aaaaacgtct atgtcattcg attgatgaag tgtggtcata cattgaagaa
    27601 tatcatgaca aacgtgtgga tttgccctat gaaatcgatg gaatcgtcat caaagtgaat
    27661 gaattctcgc tacaagatca acttggtttt actatcaaag caccgagatg ggcagcagct
    27721 tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa acatcgaatg gacagtcggt
    27781 cgaacagggg tagtaacgcc aacagctatc atgacaccag tccgagtagc aggaacgaca
    27841 gtaagccgtg ccagcctgca taacggtgat tatattaagt tgaaggatat ccgcttgaaa
    27901 gatacagttc tgatttataa agcgggagac atcatcccag aagtttcgca agtcgtgtta
    27961 gataaacgac ctaaagacag cgaagaatac caattgccta cacattgtcc agtttgtgga
    28021 agtgaacttg tccatttgga tgaagaagta gccttacggt gcatcaatcc taaatgccct
    28081 gctcaaatga aagaaggttt gaaccacttt gtttcaagaa atgcgatgaa tatcgatggg
    28141 ttggggccgc gcgtactaga acagatgtat gataaaaaac tagtagcaga tgttgctgat
    28201 ctttacaaat tgacagaaga agagttattg acgttggaca aaataaaaga aaaatcagca
    28261 aataatattt tgacagcaat cgacaacagc aaagataatt cggtcgagcg gttgattttc
    28321 gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa ttttagcaga acatttcggt
    28381 gatctagaaa cattgagtaa aagtgactat gagtcgatta ttgcgcttga tacgatcggt
    28441 gatatcattg ccgacagtgt agtcacttat ttcagtaacg aagaagtcca tgaattgatg
    28501 aatgaattaa aacaagcagg cgtcaatttc gaatacaaag ggcttcgaaa tgcccaattg
    28561 caagaagtcg agtctccttt taaagaaaaa acagtagtcc ttactggtaa actaacacga
    28621 tttactcgtg aagaagcaaa agagacgatc gaaaatcttg gcgggaaagt gactggtagc
    28681 gtatcgaaaa aaacggatat cgtggtagca ggagaagatg ctggcagcaa gctgaccaaa
    28741 gcacaagaat taggaatcga agtttggaca gaagatcaaa tggcagatgc attggctaaa
    28801 agtcgctcag tagaagaatg aaaaatgagt tttttcagac aaatttcaat aaacaatttc
    28861 agacacggca ctcttgtgtt aaaatagaga gcaaagtttt taactcgttt agaaagaagg
    28921 ggtagtatgg cgattagtga agaacaagtg aaacacgttg ccaaattagc aaaacttgcg
    28981 ttcgcagatg aagaacttgc aagttttacc gaccaattag gtaaaatcat cgatatggtt
    29041 gaaatgctgg aagaagtaga tacggaaggt gttccattta cgtcgaatgt ggttgaaaca
    29101 gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga accgcgatga attattgaaa
    29161 aatgtgccgg aaacagaaga cggctttatc aaagtacccg caatcatcga taatggggag
    29221 gctggcgcat aatgacagaa ttacacaatc gttctctaga agaactgcat catatgcttg
    29281 tatcgaaaga aatcactgta caagatttga caaaaacaac atttgaacga atcaaagaaa
    29341 cagagcctga ggtcgaggca ttcattacat tgaacgaaga aaaagcattg gaacaggcaa
    29401 aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct tgctggtatt ccaattggta
    29461 tcaaggataa tatcgtaaca aaagatatct tgacaaccgc tgcatctaag atcctttaca
    29521 attttgaacc aatttatgat gcaacggtca tggataaaat ctatcaagca gatatgatcc
    29581 ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg aagtacagaa acttcttatt
    29641 tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc tggtggctct tctggtggtt
    29701 ctgcagcagc agttgcagct ggacaaattc cagtttcttt aggtagtgat actgggggaa
    29761 gtatccgcca accagcagct tttaacggca tcgtagggat gaaacctact tatggccgtg
    29821 tctcacgttt tggtttgatt gcctttgctt catctcttga ccaaatcgga ccaatgaccc
    29881 gtacggtgaa agataatgca ttagcattga cagctatcag cggttatgac gaaaaagacg
    29941 gtacttcttc aggcgtttca gttcctgatt ttgcagaggg actgacaggc gacatcaaag
    30001 gaatgaagat tgctttacct aaagaatact taggcgaagg tgtggcccct ggcgtaaaag
    30061 aagctgtatt gaaagcagca gaaacattcc gttcattagg tgctgttgtg gaagaagtca
    30121 gcttgcctca ttcaaaatac ggtgtggctg tttattatat cattgcctca tcagaagcaa
    30181 gttccaattt gcaacgattc gatggtatcc gctacggtta tcgctctgaa aatgtgaaat
    30241 cattagatga tgtatatgtc aactcacgtt cagaaggatt cggtgaagaa gtaaaacgcc
    30301 gtatcatgtt aggtacattc tccttaagcg cgggttatta tgacgcttac ttcaaaaaag
    30361 caggacaagt ccgtacattg atcaaacgag attttgaaaa agtctttgca gattatgact
    30421 tgatcatcgg gccatcttca ccaacagttg cattcggaat cggtgagaac attaacgatc
    30481 caatcacaat gtacatgagt gacatcttaa cgatccctgt caacttggct ggacttccag
    30541 gtatgtctgt accggcaggt ttatcagaag gattaccagt tggattgcag atcatcggta
    30601 attattttga tgaaaaaacg atgtatcaag cggcatatgc gtttgaacaa gcaacagaat
    30661 tccataaaca acaaccagct attttaggag ggaaagacga atgaattttg aaacagtcat
    30721 tggactagaa gtccacgtcg agttaaaaac caactcaaaa attttctctc cagcgccagc
    30781 tcattttggt gcagaaccaa acagcaatac aaacgtgatc gactggggtt atccaggcgt
    30841 gctgccagta atgaataaag ctgcgctgga attcgggatg aaagcagctt tggcattaaa
    30901 ctgtgaaatc tctaaagata ctcattttga ccggaaaaat tacttttacc cagataatcc
    30961 aaaagcatat caaatttccc aatttgatca accaatcggt catgacggat ggatcgaaat
    31021 cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt gtccatttag aagaagatgc
    31081 tggaaaaaac atgcatggta ttggcggtta ttcttacgtt gatttgaacc gacaaggcac
    31141 gcctttgatc gaaatcgttt ctgaagctga tatgcgttca ccagaagaag catacgctta
    31201 tctagaagca ttgcgatcaa ttatccaatt tacagaagtc agcgatgtga aaatggaaga
    31261 aggctctatg cgctgtgatg ccaatatttc tcttcgccct tatggacaag aagagttcgg
    31321 tacgaaagca gaattgaaaa acttgaactc tttatctttc gtgaaaaaag gtttagcttt
    31381 tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga gaaatccaac aagaaacacg
    31441 ccgttttgac gaaacaacga ataaaacagt tttgatgcgt gtcaaagaag ggtctagtga
    31501 ttatcgttac ttcccagagc ctgatatccc gaaattcgtg atcgatgacg aatggattca
    31561 aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt cgcgaacgct atatccgtga
    31621 gtttggactt ccagagtatg acgcaatggt attgacactt acaaaagaaa tgtctgattt
    31681 ttttgaagct gttttggaaa atggtgcaga tgccaagcaa gcatctaact ggttgatggg
    31741 tgaagtttct gcttacttga acagtgagaa actggaatta gctgagacaa aattgacacc
    31801 tgagaactta gcaggaatga ttcgtttgat cgccgatgga acgatcagct caaaaatcgc
    31861 caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa gcccaagaag tagttgaaaa
    31921 aaatggttgg gtgcaattat ctgatccgaa caaactactg ccaatcatta atgaaatttt
    31981 ggataacaac caacaatctg tcgatgattt caaaaatgga aaagatcgtg cggttggctt
    32041 cctagtcggt caaatcatga aagcaactaa aggacaagca aatcctggtg tcgtgaataa
    32101 gctacttaaa gatgaattag cgaaacgata gaggctgaaa atgaaaaaag cacgcgtaat
    32161 ttataacccc acctcgggaa aagaattatt gaaaaaaaat ttggcggata tcctacaggc
    32221 attagaagaa gcaggatttg aagccagcgc ctatgcaact acaccagaac cagattcagc
    32281 caagaatgaa gcatgtagag tcgcagaact aggttttgat ctagtagtgg cagctggtgg
    32341 cgatggcaca atcaacgaag tggttaatgg aatcgctccg cttgaacacc gtcctaaaat
    32401 ggccatcatc ccagctggta caacgaacga ctatgctcga gcattaaaaa ttcctagaga
    32461 taatattaaa gcagcagcag aagtgataaa aaaacaccaa accgtaaaaa tggacatcgg
    32521 cctttctgat aacagttact tcatcaatat cgctgcaggt ggctatttga cagaattgac
    32581 ttatgaagtt ccatcagaac taaagagtat ttttggttat cttgcttatc ttgcgaaagg
    32641 agcagagatg ctccctcgag taaagccaat caaaatgcgc ttgaagtatg atgaagggga
    32701 atatatcgga aatgcttcga tgtttttcct agggctgacg aactctgttg gaggattcga
    32761 acagatcgcg ccagatgcca aattggatga tgggaagttt tctttaatca ttgtgaagac
    32821 agcaaatatc tttgagatct tgcatattgc agcactcatg ctgaatggcg gtcgacatgt
    32881 cgatgatcca agaataatct ataccaaaac aagcagtttg ttcgttgaaa cagaagatga
    32941 tatcaatcga ccagtaatga tcaacttaga tggtgaatac ggcggggaag caccgatgca
    33001 tttccaaaat cttcatcaac acattgagtt ctttgccaat accgatcaga ttcctgatga
    33061 agcaatcaca ggtacagatg aagaagaatt agaggttgtc agcaaagaat tcgtaaaaga
    33121 agtcgaacgt ttaacggatg aagatatcga cggtgacgga aaaatagcag acgaaacacc
    33181 taatcaaaag aattaatcaa aagccggtgc tgaacgtagt aaggtcagtg ccggttttag
    33241 ttgtgtaatt agctgattta tagtagagtt cagtaggatt gaaaaatagg cttaaaggag
    33301 caaaatatga cagaacagca aatagtaaag aaaaacgaac gatatacagt cgaaatcgtc
    33361 gacttgagtt atgaaggaat gggggtagca aaaatcgatg gttatccatt atttatcgaa
    33421 aatgcactcc ctggagaaga agtagaaata ctagtagtaa aagcaggcaa taagtttgga
    33481 tatggaaaag tcgaaacatt tattacacaa tcacctgatc gtcagcctgt agaaaacaat
    33541 gcattacttc gcataggtat cgcaccttta gcccatttga agtatgaaca acagcttgtc
    33601 ttcaaacaaa aacaagtaga gcatgtaatg gcgaagattg ctaagatgcc agaagttcct
    33661 gtacttccaa cggtcggaat ggcagaacca ttcggctacc gtaataaagc acagatccct
    33721 gttcgccgta tcgatggaca gttagccacc ggtttttaca aaaagaatag ccatgatcta
    33781 gtccctatcg aagattttta tatccaagat cctgtcatcg atcaagcaat caaagtagtc
    33841 agagatatct tgcaacggtt ccaagtacga ggatataatg aagaaaaaaa tgaaggacag
    33901 cttcgtcaca tcattatccg tcgtggtcat tattcccatg aaatgatggt cgttttagtt
    33961 actcgtaaag aaaaattctt taaatccaag gaaattgctg aagctattca tgaagaattg
    34021 ccagaagtgg tttcggtcat ccaaaatatc aatgaagaaa aaacaaatgt gattttagga
    34081 gacaaagaaa aagttttata tggcagagac tatatcgaag atcagctatt aggaaaaacc
    34141 tatcgaatct cagcaaagtc attttatcaa gtaaatactc aacaagcaga gtatctgtac
    34201 caaacagcca tcgagtttgc cgagttagat aaaaaagaca ttgtgatcga cgcttattca
    34261 ggaataggta cgattggctt gtctttagca gacagagtgg ataaggtgta tgggatggaa
    34321 gttattccag aagcaattga agatgcacag ttcaatgctt taaataataa aatcgaaaat
    34381 gttcattatg aagtcggaaa agcagaaaaa gttatgaaaa aatggcaaga aaaagggatc
    34441 aaaccatctg tgatcgtagt tgaccctcca cgaaaaggat tagatcatca ttttattgaa
    34501 tcagcaatcg aaatgtcacc agaacgaatc gtctatatct catgtaatcc ggcaactttt
    34561 gcaagagatg ctaagttatt tgcagaagca ggttatgccg tgaagaaagt tcagcctgta
    34621 gatcttttcc cgcaaaccca tcatatcgaa ttagtcgctt cattcgttaa aggagaataa
    34681 aaatggacca aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt ggaggccgat
    34741 ccaaacaaat ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc cctaaaatct
    34801 ttaataaata gacgtgctaa agaaagacta agaagtgtta acttttgtta ttgtttctta
    34861 gtctttttag ttagtctaca ttattttggc gatttgccaa taaggactgt tgagttgtgc
    34921 aaaatcaaat ctgggtcaga atgaacgtca ttctgtctca gatttttcgt tatttcaatt
    34981 tgattaactg attgatcaaa aagattttta ttttcatatt ctcaaatgat tcaacactaa
    35041 atatacatgt ttgacgcgga aattattatt cgattttggc ggcatcaggg gggatgacga
    35101 cgtagcagac ccattatttt actcaagaaa atgtccgtgc ctattattta gaatacgata
    35161 gtgaacgtgc tgggagtttt gttcttttag agaaagtctc tgaagataaa ttagttgtct
    35221 tagattttga ctgtaacgaa aaatggcgaa atggaagagc accagcaagt gattgaccgg
    35281 atctacgaag cgacacgata tctgcctttg gatcgtttgt gcttaagtcc tcaatgtgga
    35341 tttgcttcaa cagaagaaga aaatgttttg tttgaagaac aatggaaaaa atttcttttg
    35401 tcaaaagcat tgcagaagaa gtttggggaa aatcattaaa tcatctagga gagcttgata
    35461 ggaaataaca ctatggtata atcagaatgc ttaatactaa ctactgaggg agccttatga
    35521 aaaagaaaat tcttaaattt atcggatggt tcttcggaat cattcttggg atcgcgtttg
    35581 caatctttct tgccttccag ctttccccca gacctggagc attcttgatc aaccatatgt
    35641 tttcaaatac tgttcaaatc actgataaaa aaacgttcga tcaagcagaa aaaaacgtgg
    35701 cgaaaaaaac agatttgacg tatgagtcca agtataaaga gaatacttat gacatctact
    35761 atccaaaaaa cagcaagggg cctgttcctg ttctattttg ggtacatggt ggaggattcg
    35821 tcggcggaga taaatcgggt gtcaaagaat ttgcgacaaa attagtttca gacgccaaca
    35881 tcgcagtggt tgcgatgaat tatgaattag cgcctgattc agaataccct aatcaagttc
    35941 ttcaagtgaa tgaactgatc aaagatttat tagctgaaga aaaagacgac aaattgctag
    36001 acatggaaca attatttttt ggtggagata gtgcaggttc acaaatcgca ttgcaatatg
    36061 cagctataca aacgaatgat gcgtatgcta aacaaatgaa aatcaaacag ctacttccaa
    36121 aagatagtct gaaaggaacc atttcttatt gcggacctgt tgatttgaag ctaacagcac
    36181 agcagcattc ggatgatcga tttatgaagt ttttcgtcaa aaccgttgcg tggtcgttga
    36241 ttggaacaaa agattggaaa acgagtgagc aattgaaaga agctagtgtg gctgaccatg
    36301 tttctaagga atttccgcca acgtatataa ctgacggaaa tgcatactca ttccaggagc
    36361 aaggtcaagc attagaaaat cggttgacag aattaaaggt acccgtgcaa tccttgtttt
    36421 atacagatac aaaaaaagag attacccatg aataccaatt tgattacacg ctaaaagaat
    36481 cgcaaaattg ttaccagcag acattggatt ttgtgaataa gtataagtaa gaggttgtga
    36541 aaaagccgtt tcgttttgag tatgaagaaa cgaagcggct tttcacaacc tctctttcaa
    36601 tacctttttg ttcttgggat aaaactttcc ctaacaatca atcgtgcagg ataagtcaaa
    36661 atgattggtt cgtcccgtat accgtcaatt cgctccaatg ccattcgaac agctgcttgc
    36721 ccaattgatt tcgcattgat ttgaacactg gtcaagccag gggagaggtt tgcgattgtc
    36781 tcgatattat caaagcttgc tacaacaatc tcttctccaa tttggatatt tttttgtttc
    36841 aatgcccgat agacaccaat tgccattgga tcgctcccga caagaatggc agtaggaaga
    36901 ggggattcat tttctaatag attagtcgca agtacctcac cttgtttttc tgtccagcca
    36961 cctagatgat aagacatata tttttctaag tgatgatcga tcataaaatg ctgataggca
    37021 cgcatcctct tttcatttat agaaatctgc ttcccaccga attcatccat atcgacttta
    37081 tatcccccga tataggctat tcttttatga ccattttcta agaacaattc cagtacggtt
    37141 ttagtcatcc gttcaagatc tgcatacacc atatcgacct cttgctggat gtcagggtta
    37201 tccacgacga ctatttgcat gttttgtttt aacaaggcgg taatcgattc tttcgtaaca
    37261 gtcccaacaa cgatcaccgc acctaactga tctagatctt cccattgttt aggattatca
    37321 gatagattat agattcgatt catccctaaa tgcagacgtc gagcttcttc ttccatccct
    37381 actcgcaaat gtttgtaata gatatcctgt tcttcgctcg ctttgccgat cgtagagatc
    37441 acaccaatcg aaggcattct ctttttgata tatttacgag tactgatgtc ataatccaac
    37501 gcagcagctg cctctactat ccgttgccga gtagtctgag ccacatggat cgtctgatcg
    37561 ttattcaaca cacgggaaac tgtagcagga gagacatttg cctttcgtgc gacatctcta
    37621 attccaacca tttttacaca ctcctcaaaa aaataaaaaa tttttattga caaaattgtt
    37681 tagtaaacag tatacaggaa ttataaaaaa aagtaaacgg tttccgtttt gttgggagtg
    37741 aaatgatgga tattacgcat agaaaaacag ataaaaattt catcttattt gatgaaaaga
    37801 gccgagtctt ccatttacga aatacatttc tttcttatct gataaagata gaagaaagca
    37861 atgtattggc gcatatttat tttggcaaac cagtcaagca gtacaaagac aataaaaatt
    37921 atccccgaag agatcgtgga ttttcaggaa atgttccctt aaatcctgac cggtctctgt
    37981 cgaaagatac actaccgcaa gaatactctt ctcatggcag catggacttt cgaacgcccg
    38041 catcgatcat tcagcgcaag aatggatcag acttactgga tctgagatat gacagccatt
    38101 atattacaga tgggaagcca gagatcgaag gcttgcctca aacttatgtg atggacaagt
    38161 cagaggcaca aacgttggtg atttcactta aggatagaga aacagcgatt tatttcgatt
    38221 tgttctatac catatttact gatagagcag tgatcacacg atctgtaaag ataagaaatg
    38281 aaaccggaga aacaatcaaa ctagaaaaag cggctagttt ccagctagac tttgcccata
    38341 ctaggcgatt tgatgaagtc attgctttgc caggagcaca tgtcaatgaa agacagattt
    38401 cacggcaatc tgttttatct ggaacaaaag tatttgaaag ccgtcgagga acgagcagtc
    38461 accatatgaa taactttatt gctttagttc atcatcatac gacagaaaat actggagaag
    38521 cgatcggact tcaatttgtt tattccggca accattcttt cgaacttgaa aaagatcaga
    38581 tcaatcaatt gagagtcgtc ggtgggatca acagccatcg tttttcgtgg gaattgaatg
    38641 caggccagtc ttttcaaaca cctgaaatga tcctttctta ctcaagtcag ggattgaaca
    38701 aaatgagtca gatccaccat gagttgctac gtgaaagaat cgcaagaggt cgtcatcaat
    38761 ttgctgaacg gccgattctg gtcaataact gggaagccac ttactttgac ttcaatagtg
    38821 aaaaaatcaa ggcgatcatt gatgaagcaa aggagctagg catcgaaatg ttcgtattag
    38881 atgatggctg gtttggaaaa agagatgcgg acaattcttc attaggagac tggttcgagt
    38941 atgaaggcaa gttgacaaat ggattacgtg aaatagcaga ttatgctcat agcaaaggat
    39001 tgaaatttgg tttatggttt gaacctgaaa tgatctcggt tgattctgaa ctgtatcgaa
    39061 cacatcctga ttttcttatg caggagccag gtagaatgcc ttctgcttcc agatcacaac
    39121 atgtactgga ttttacaagg ttagatgttc ggcagacgat tgagaaacaa atgagaaaga
    39181 ttttagatac gattcctctt gactacatca aatgggatat gaaccgttcg ctttcagatg
    39241 tctattcgat tacccttgac ccacagagac aaggagaagt agcgcatcgt tatatgctcg
    39301 ggctttatga actacttgaa catttgacaa ctgattatcc ggaaatctta tgggaaggct
    39361 gttctggtgg aggtggacgg tttgatgccg ggtttattta ttatatgccg cagtcatgga
    39421 caagtgataa cacggatgca gtggagcgta tgaaaatcca atatggaaca agccttgctt
    39481 atccgatttc gtccatcaca gctcatgtat cagctgtacc aaatcatcag accgacagat
    39541 ctacttcgtt gaaaacaaga ggagaaacag ctatgagcgc tgtttttggt tatgaactgg
    39601 atttgacaaa gctgtctcct gaagaaaaaa agcaagtaaa agaccaaatt atatcctatc
    39661 aaacgattcg tccggtgatc cagtatggcc attattatcg tttggcgagt ccttttgaag
    39721 aaaatattgc cgcatggatg tttgtgtctc ctaaacaaga tgaagctatt gtgtttttag
    39781 ggaggatact agcttcagct caaccagcgt tccatgaagt atatctgatg gggttagatg
    39841 atgaggcact ttatcaggaa cagacctcga agcggatatt ttcgggggcc gaattgatga
    39901 cagttggact ttacttcccc gattttcaag gtgatttcca aacagaactg cttcatttca
    39961 aaaagttatg agagagaagg aaaaaagtat gaaagcaaat ataaaatggt tagatgatcc
    40021 acaagttttt agagtgaacc aatcaaatgc acatagcgat catgtttgtt atgcaacaag
    40081 ggaagaagta gaaaccacat cctcccttaa acggtcttta aatggatcat ggcgtttttg
    40141 ttattcgcca aatccttcgg tacgaccaaa aacattttat aaaaaagatt ttgatgcctg
    40201 ttcttttgat gaaatcactg ttcctagtca tattgaatta gcaaattatg acaagatcca
    40261 ttacatcaat acgatgtatc cgtgggaggg acatgtattt cgccgtcccc ctttcacgac
    40321 aagccctgag aaagaggcgg aagggatctt ttcagctgct aaagacaatc cggttggttc
    40381 gtatttgacg acttttcgat tagaagaagg tctaaaggga aaacaaatct cgattcattt
    40441 tgctggtgta gaacaagcaa tgtttatttg gttgaatggt cattttatcg ggtatgccga
    40501 agattcgttc actccgtctg aatttgattt aacaccttat ttggaagaag aaaatattct
    40561 agctgtggag gtttacaaaa gaagtacagc tgcattcttg gaagatcagg actttttccg
    40621 attttttggg atcttcagag atgtcttctt agttgctcga ggacaaaacc atttagaaga
    40681 tctttggatc aaacctcgct tagatgacaa atgtcaaaat ggcaaacttg aagtagattt
    40741 gtcttttgag caagtagcca aaaacagtca gctgaatgta gcgatactag atcctgatgg
    40801 gaaggaaatc tatcaacagc aatatccgtt aaaagagcaa atgacgattc gcatcgatgg
    40861 atttgaaaat gtggcactgt gggggcattt tcagcccaat ttatataagc ttgaattgga
    40921 gacgatagac gaagaaaaaa gagtaacgga gtatattgac tattccttcg gttttcgtaa
    40981 aatcgaaatc aaagataaaa tcatctactt caatggaaaa cggatgatta tcaacggggt
    41041 aaatcgacat gagtggcatc cgaaaaaagg acgcgccatc actatagaag atatggaaaa
    41101 agatttggct atcataaaaa atgcaaagat caacggcgta cggacatctc attaccccaa
    41161 ccagatcact tggtattatt tatgcgacca agcaggaatc catgtgatgg cggaaacgaa
    41221 cctcgaatcc catggctcat ggcaaaaaat gggcgcattt gagccttctt acaacgtacc
    41281 aggtgcagta cctcagtggc aggcggctgt tcttgatcga gcaaaaacga atttcgaaac
    41341 attaaaaaac catccaagta ttcttttttg gtctcttgga aatgaatctt atgcggaaga
    41401 aaacattcga caaatgaacg attacttcaa aaaagtagac cccgatcgac ttgtccatta
    41461 tgaagggaat tttccaaatc cagcattcga agattcgatt tcagatgtaa aaagctgcat
    41521 gtatgcgcca ccagaagaga tcattgacta ccttgaaaat aaagcggaaa aaccgttcat
    41581 cttatgtgaa tatatgcatg acatggggaa ttcgttaggt ggaatggatt cttacatgac
    41641 gttgcttgat cgttatgaaa tgtaccaagg aggctatata tgggatttta aagatcaggc
    41701 gatccaagtg actgatccaa tcacaggcag agaagtgatg cgctatggcg gtgactttga
    41761 cgataaacca agtgactatg aattctcagg gaatgggatc gtttttgcag atggacaaga
    41821 aaaacccgcc atgcaggagg taagatatta ttatgaaaaa tacagtaaat aaaagtcata
    41881 tggatacgga aaaagttgca atcgtcttcg gcgactgtac attaggtgtc aaatcgggga
    41941 atacgcatta tattttttct tatacaagag gcggactgga atcgctcaat aaaaacggaa
    42001 aagaatggct ataccgggaa acacttccag ttttttggcg agcaacgacc gataatgaca
    42061 gaggtagcgg atttggattt aaatccggta tgtggttagg agccggattg tatccaaaag
    42121 tcaaagaaat agaagtactg gtggacaata aaaagatcga tttgccgatt gctccagtaa
    42181 ataatcaata ttcaagtaat gaatacgctg aaacggttga aatcattttt acactggaaa
    42241 tcaacacagt accaaaaacc caagtgacaa tttcctacca aatcgctgga gagggtaaaa
    42301 tgcttgtcaa tatgcattat tatggacaaa aaggattacc ggaattaccc gcattgggtt
    42361 taaggttcat tatgccaacg aaagcaaaat tttttgagta tgaaggccta tcaggggaaa
    42421 catatccgga tcggatggct ggtggtgtcg aaggtattta taaagtggca ggactccccg
    42481 tttccccata ccttgttccc caagaatgcg gcatgcatat ggaaacgaaa tgggtagaac
    42541 taacaagaaa tcaaacgttg gataatacgg ataaagatca agaagatttt tcgttgcgat
    42601 ttgaacagca ggatacgcca tttgcttttt ccgctttacc attcaaagca gaagaactgg
    42661 aacaggcaac acatatagaa gaactaccgc cagaaagacg aacggttctt acgatttttg
    42721 caaaagtaag aggagtcggg ggtatcaata gctggggagc ggatgttgag tcaccttacc
    42781 gaatcagcag cgaagaggac catgaaatcg gctttattat tcagtagcaa tctttagatt
    42841 attatttgaa aaacggagtt tacaagacaa agctgcttta ttctatatgt catgaacaag
    42901 agagtaaaaa agttctcaga tacttttgtt catgactatt ttcttcaata aaatcaattg
    42961 ttatttttct gaaaaacaaa gttaactatc agaaaattaa aaaaagtttc ataaaaatga
    43021 ttgacagtcg atttttttat gtctatacta ggtacaattc ttacatcagt ttggagcgat
    43081 tttttatgaa atctagaaaa cttacaaaac tgaatacgta ttactttagc tattttagat
    43141 aggtttgtat tcatgacttc gtgggtacaa acaaaccagg tttgtatcta tgatggctaa
    43201 agatttttaa acacgactta cagccacgta gagaaattca tctatagtgg ctattttata
    43261 tttttgcagc gctttttaag gctcgttgaa gatgttcact gtagaggcaa tttcctacag
    43321 ggacatcttt ttttgttgat tatctgggtc ataagtagga aagaaggagc gaaaatgaga
    43381 acaacaggac acggtcattt agtaggaatt aatgtaaaac caccttgaaa aagtagagaa
    43441 aatcagccaa gatagataag taaacaaaag aaagcgggaa aaacaatgat agtaattatg
    43501 aaaaaagaag caacggaaga acaaataaaa aaagtcgtcg atcatatcga aaaagaagga
    43561 ttcaaagtct caatcgatca aggaacagaa cgagtagtga ttggactaaa aggtgatacg
    43621 cgaagtttac aagaaggagc atttacacgc tatgaaggag tagaaaatgc tgtgcgtatt
    43681 ttaaacacat acaaattgac ttctcgagaa ttccatcctg gcaatacagt cgtggatgta
    43741 gacggagtaa aaattggtga cggcagtttc gtgacgatgg ctggtccttg ttctatcgaa
    43801 ggattagacc aaatcagaga atgtgcgcga atggccaaag ccgggggcgc aaaaattctg
    43861 cgaggtggtg ccttcaaacc acgtacttct ccatatgctt tccaaggttt agaagaagaa
    43921 ggattgaaat acatccgtca agcggctgat gaatttggaa tgaaagtgat tactgaagtg
    43981 atggatgaag ggcatatcga tatggtggct gagtacagcg atatcctaca gattggcgca
    44041 cgtaatatgc aaaattttaa actgctatct gctgtcggta aaacaggtaa acctgttgga
    44101 ttgaaacgtg gaatttccgg tacgatcaat gaatggctta atgctgcaga atacattgca
    44161 gtcgaagaca aatctcctgt catttttatc gaacgtggga ttcggactta cgaaacagcg
    44221 acacgtaata cgtttgattt aagtgctgtc ccattgatga aaaaattaac tcatttccct
    44281 gtgattgttg atccaagtca tggtacagga atttgggaac ttgtaccacc aatggctcgt
    44341 gcgggtgtgg caagcggagc agatggcatg atcgtagaga tccatcctga tcctgcaaat
    44401 gcttggtcag acggtcccca atcgttgaat gaaaagactt actcacgcat gatgaaagaa
    44461 gttgcgatca tgaaagaagc aatggaaaag attcagacac tcgattaatt ccagcttttt
    44521 aaaagaaaga agtgaaacga atgcttactg tacatgttga taaccaaaaa tacgatattc
    44581 atattgaaaa aggcgcacta gaacaggctg gatcatggct tcaaacgatt tggagacctc
    44641 aaaaagtagc tgtgatcact gatacaaacg ttgctccact atatgcagaa aaaatcatgt
    44701 tccaattgag acaagctaat tttgaaccag tcctttcaat cgtaccggcg ggtgagacca
    44761 gtaaatcttt agaacaagca gctgagttat acgatttttt agcagataat gattttaccc
    44821 gcagcgatgg gatacttgca cttggtggag gaatggtggg tgatctagca gcattcgtgg
    44881 cctcgacgtt catgcgtggg atccatttcg ttcaaattcc gactacgcta cttgcacaag
    44941 tagattctag tattggcggg aagacagcag tcaatacaaa caaagcgaaa aaccttgtcg
    45001 gaacatttag ccagccagct ggtgtattga ttgatcctga ggttctaaaa actttgccgg
    45061 tacgtcgttt acgagaagga atcgctgaaa tcatcaaatc agcagctata gcagatgcaa
    45121 cgctttggaa acagctgaac gagctgaaag acgaaaaaga tcttctggaa catgctgttc
    45181 caatcattac tgctacgttg gaaatcaaac gaaaagtagt ggaagaagat gtctttgacc
    45241 aaggaaatcg acttatttta aattttggtc atacaattgg ccatgcaatt gaaaatacag
    45301 caggatatgg ggtagtcagt catggagaag gagtagctat tggaatggta gccatcactt
    45361 ctcatgcaga aaaaatcggc ttgtctccaa ctggatcgac agctgcttta gaaaggatac
    45421 ttcaaaaatt ccatctgccg attacgtacg atctaagtca tccagatgaa ttattccacg
    45481 cgataaccca tgataagaaa gcaagaggaa gccaattgaa tatcatcttg ctagaaaaga
    45541 ttgggcaagc aaaaatcgtt cctgttccca cagagacatt caaagattac ttaggaggac
    45601 tgtaagatgc gttatatgac tgcgggagaa tcccatggac cagaattaac agccattatt
    45661 gaaggactac cagcaggatt acctctgtca gaagaagaaa tcaatcacga actcgctcgc
    45721 agacaagctg gttacggcag aggaggaagg atgaagatcg aaaaagatcg tgttcggatc
    45781 acgtctggca tccgtcacgg caaaacaatt ggttcacctg tcactttagt ggtagaaaat
    45841 aaagattgga aaaattggac aactgtcatg tctgtagcag aagtgcctga aaaagaccaa
    45901 tcactgcggc gagttgcacg tccgcgtcca ggacacgcgg atttggtagg cggtatgaaa
    45961 taccatcatc gagatcttcg gaatgtgctt gaacggtcct ctgcaagaga gacgaccatg
    46021 cgtgtagcaa tcggcgcaat tgccaagaaa attctggaag aacttgacat catcgtagca
    46081 ggacacgtga tttgtcttgg tggaatccaa gcagaaatac ctggaaatat ctcgattcaa
    46141 gaaatcaaag aaatttctga agcgtcagaa gtacgagttg ttgatccagc agttgagcaa
    46201 agaataaaag atttgatcga tcagacaaag aaaaatggag atacgatcgg aggaattgta
    46261 gaagtgctcg ttggaggcgt tccagctgga ttaggaagct acgtacaatg ggacacgaag
    46321 ctagatgcca aaatcgcaca agctgtggtt agtatcaatg cctttaaagg cgtagaattt
    46381 ggggtcggat tcacttctgg ttcattacca ggctcagaag tgatggatga gatcctatgg
    46441 gaagaagaaa gaggctattt ccgacgtacc aataatctag gcggcttcga aggcggaatg
    46501 accaatggcg aaccacttgt cattcgcggg gtcatgaaac ccattcctac attgtacaag
    46561 ccacttcaaa gtgttgatat cgatacaaaa gaaccttaca aagcaagtgt agaacgttcg
    46621 gatagtacag cagttcctgc agctagcgtc gttgcggaaa atgttgtagc cactgtggta
    46681 gcgacagagc tattgtcaaa attcccaagt gattcgcttg ttgagttgaa agctgcagtg
    46741 aaggagtacc gtgactactt gaaacattac taaaaagggg gcaaataaga tggcacaaca
    46801 tgtttttatc gtggggcttg gactgattgg tgcttctatg gcactttgta ttcgtcagac
    46861 caatccagaa ctgatagtag ttggttggga tagtcagtca aaaacccgtg agcaagcagt
    46921 agaacagcag atcgttgatt acgttgcaag tgattttgaa acaggtgcag aacaagcaga
    46981 cgttatctta ttagccgttc ccgtccgtac aaccttagct tatcttgatg tccttgaaag
    47041 aatcaacttg tctgatcacg tcatcattac ggatgtcagc agtacgaagc agcaagtcgt
    47101 gacatccgct gagcagaaaa atcttcggtt tgtcggtgga catccaatgg ctggttctca
    47161 taaatcaggt atacaggcag cagatgctaa actatttgaa aatgcctatt acatctttac
    47221 acctttggaa aagacgaaaa aagatgttga aaaactgcaa gaactatttt gcggaaccaa
    47281 agcaaaatat gtaactctta cagcaagaga acatgaccgt ataacaggaa tgctaagtca
    47341 ttttccacat attctggcag ctggcttagt caatcaggca gaacaattca atcaagagta
    47401 tccgcgagcc aaacaactgg cagcaggagg ctttcgagat atcactagga tcgcttcctc
    47461 agatcctgtc atgtggacag atattttgtt gagcaataag cagatattat tggaacgatt
    47521 ggcagattgg caacaagaaa tgacacaaat agcagaatgg atcatgactg agaaccaatc
    47581 ggaaatcttt tcttttttca atcgggcaaa agaaagtcga gaccagttgc ctgtccataa
    47641 gcaaggcgcg attcctgcct tttttgattt atttatcgat gtgccagatg aacctggcgt
    47701 tattgcagaa gtaactggac tgatcggaaa agcgggagtt tctttgatta atttgaagat
    47761 ccaagagacc agggaagata ttttaggaat actgcaaatc agttttaaaa accaacagga
    47821 tttgttacaa gcaaaaagaa tcattatgtc taataccagt tatcattgct ggatcaaata
    47881 ggaggctgga taaatgcaat tattacaaca aatacatgga ttaagaggga ctgttaggat
    47941 accagcagat aaatcaattt cccatcgcag catcatgttt ggagcaattg ctgaaggaac
    48001 gacgactata caaaattttt tgcgcgcaga agattgtctg agtactttac atgccttcca
    48061 acaattaggt gtcgagatcg aagaagagga agaggtgatc aagattcatg gtcgcggtag
    48121 ccactcattt gtccaaccaa ccgaatcaat tgacatggga aactccggta cgaccatacg
    48181 tttattgatg ggtattttgg ctggacagcc ttttacgact actctgttcg gggatgcttc
    48241 gttgtctaaa cgtccaatgg ggcgagtgat ggaaccttta cgcgagatgg gcgctgactt
    48301 gcaaggagaa gaaaatgacc agtatctacc aatcactgtg acaggaaccc gctctttatc
    48361 acctatccga tacaatatgc ctgtagccag tgcgcaagtc aaatctgctt tgctgtttgc
    48421 ggcattacaa gcagaaggca catccgtaat cgttgaaaaa gaacgttccc gtaaccatac
    48481 ggaagaaatg attcgtcaat ttggcggaag gatcacagtg gaagataaaa cgatcatagt
    48541 gaccggaccg caaaaattaa ctggtcaaca gataactgtt ccaggtgata tttcatcagc
    48601 tgctttcttt ctagcagcag gacttcttgt tccggaaagc caactgttgt taaaaaatgt
    48661 cggggtcaat ccaacaagaa ctggtatctt agatgtgttg gaggagatgg gcgcagcgat
    48721 tacccagacg aatcacaatg aacagaacca atcggctgat ttaagtgtca agacgagtca
    48781 tttgaagaaa gcaacgattg gtggagaaat cattccgcgt ctgattgatg aattgcccat
    48841 tctagcttta gtggctactc aagcagaagg aatcacgatc atcaaagacg cagaagaatt
    48901 aaaagtaaaa gaaaccaaca gaattgatgc ggtggcagaa gaattacaga aaatgggagc
    48961 aaagataaaa gcgacagcag acggtctgat catccatggg ccgacacctc tgcatggcgc
    49021 aaaagtgtcc agcagaggcg atcatcgaat cggtatgatg ctgcaagtag ctgctttgat
    49081 agctgatgga ccttgcgaac tagaaggagc tgaagctgtt tcaatctctt atccggcatt
    49141 ttttgacgat ttagcagaac ttgtgtcagg aggagaagct catggctagc attttgctga
    49201 ttggtttcat gggcgctgga aaaacaacaa tcggtaaagg gttagctcaa tgtttacaaa
    49261 aagagtatgt agatttagat acaaagattg aggaacatat ccaattgtcg attgccgaat
    49321 attttcacta ttacggtgaa aaaagctttc gaaaagtgga gtcagatatt ttaagaaagc
    49381 tgtcaaatga agacaaaatt atcgcgactg gtggagggat tgtccaaagt gcagaaaatc
    49441 ggcgtttttt aaaaacacag ccgattgttc tttacttaga ggcagaagcg gactgtctag
    49501 ttgatcgtat ccaacaagat gaaacgtcta ttcgaccttt agctcttgga aaaacaagag
    49561 aagaaataaa agcattattg gcacaacgac tttcttggta tgaagaaagt gcgacacatc
    49621 gaatcaacac gaccaatcaa acaccagaaa agatcatcga taccattata gaaaggatca
    49681 aaagcatatg aacgtcagct atctagggcc agaaagttca ttcacttatc aggcggcttg
    49741 tcagctattt cctacagaac aattgactgc gtatgcttcc atccctgttt gtttgcgcgc
    49801 tttgtttcgc aaacaagtcg atctggctgt cgttcctgta gagaattcat tggaaggagc
    49861 tgttcatcac acaatcgatc ttctttccaa acatccagaa gtcgaagtta aaagtgaaat
    49921 cgttcttcct atcaagcagc agcttttagg caatccagca acaaagatta caaaaatcct
    49981 ttcacatcca caagcactag cacaatcaca acagttttta gaaacgcatt atcctaatgt
    50041 tcctttagta gcaacagaat caacaactgc tgcagcaatg tatgtagcag aacatccgaa
    50101 agaagacgca gcagcgattg cttcattaga aacagcacag catgttggat tggagatctt
    50161 agctgagaat atccaagata atgagttgaa ccagacacgt ttttggatcg tcggtgaccg
    50221 aaagatgacc agccagcagt cagctccagt aaaaatgagt gtcatcttga cactacctgc
    50281 taatcggcca gggatgcttc ataaaatgct ggcagctttt ggctggcggg aaatcaactt
    50341 aagtaagatt gaatcccgac cattgaaaac tagcttggga gaatattttt tcgtgatcga
    50401 tttgttgttg gaccgtccaa tgacattagt agagaatgcg ctccaagaaa tcaagatgct
    50461 cggaggagaa agtcaaatat tgggctgtta tccagtgctg acagtagaag agacgcgtag
    50521 atgaaagtca agaatactga ttgatttatt taacttaaaa taaaaaaatc agcgcttggc
    50581 tattctttat ttttgatatt ctgattgatt tttaggaaga agcggtagac aaatcgttac
    50641 agtcgttcct gtcccaatca ctgattggat cgttaagcca tatgcatcgc catacattag
    50701 gcgtatccgc tcatctacat ggctgatgcc gattcctgaa aaatgatcat ttttttgttt
    50761 ggtattttca gtatcgaagc catcgccgtt atcaatcact tcgaataaaa gaagctgatt
    50821 ttttgttttg ccgtacaccg tgatgaatcc ttgcgtttta ttgtgaaaag catgaagata
    50881 ggcgttttcg ataattggtt gaaggatcat attcggcata gataatgact gcagttcatc
    50941 aggaatcatt attttagccg agattctttc gccatatcgc atactcaaaa gatcgatgta
    51001 actttgtacg cttttgattt cttccttgac tggaatcact tcttcttttt tcccaatcgt
    51061 acttcttagt aaagtaatga aactgtcgat tccttgaact gcttcatctg tttttttctg
    51121 ccaaatcaaa aatttcaagc tagtcagcgt attataaata aaatggggct gtatctgtgc
    51181 ttgcatagtt tggatttcaa gcgaacgttt cttcttttcg tcttggatcg tctgttcata
    51241 atgatgctga gcattactta gcaattgatt gatcgtattc cctagcaatt ggacttcatg
    51301 gcttccttgt acaggaacag tagcttttaa gccatcttcc tctagccgtt ttagcgaatc
    51361 aatcaagcgg tagatcgatt ttgtgtgtcg atccaaatac ataaatacca atgagccagc
    51421 aaaagcaagt gtcacgatat tatacaaaat aatcggaaaa atcacgccca tttggttaga
    51481 taaagaatga agatcgactt gactgagtaa tgtacaatcc tggcggtaca gaggaagacg
    51541 ggtcaactgc tgtccatttg aaatcgttgt ttcacctact gttagttttt tgaaaatcat
    51601 tgatcggctc ccaattgtat tttcttcatt tgatgcgatg atcgtctgtt cttcgttcat
    51661 gaaggagatc ttcgatatct caggatttac tacagattga aaaagattcg ctagttgctt
    51721 ggatgagatc gataaaacag catatccgta aacgtgattc aatgtatttt tcagtttttt
    51781 gacaaagaag acgctgggtg ctttcttatc gcgaagagtc aatccgtggt gtgcaaaaag
    51841 catttgtgta tcagcagcac gttcattcac tctctgagca atcgcagatt gaaaaaaaga
    51901 atcggcagat acggcagtga gcatgtcgtt tccgacaaaa gttcttccat tttctccaaa
    51961 gatcaataca tcgtaatcga tcattggatg gatttttgaa agagagtcat tcattttttg
    52021 gattgttctt agttgctgaa gctgttccat atgttgggct ggatcattgg aaaaatagct
    52081 tttgacagct ggattagaat caaaagcggc taatgtattc tcgattgtat cattcaaact
    52141 ttcaaattca gattgaatct tttttagtac cacatcattc gattgattga acagctggat
    52201 ataggcatcc cttgatcgat gataaataaa caaagaaatg acgatggctt gcagaaaaag
    52261 accgatgaac aaaatgaaca gaagtttggc aaagagttca tgctctttga ttttttttct
    52321 cattttacaa aagttccttt acggtattga gaaggtgtca ttcctgtctc ttctttaaaa
    52381 actctggaga aataacccag ttcggtatag ccgactttct cacagatttc cgtcaaagta
    52441 agccgtgttt ccgtcaaaaa gagcttggct ttactgatac ggactttttt tagatatttt
    52501 gaaaagttga taccaaattt ttcagtaaag atggctgaga gatagctata tgaaaaatga
    52561 tatttttcgg ataaaagttg taaattgatt tctttctcat aattttcctg gacgaactcg
    52621 tagatcaaag tcaagatatc gaaattgtct ttttttgcat ttgcagaaaa gtgggtgagt
    52681 ttctcaagtg tttctttgac aagaatagaa aaatcttcga aatactcagc atcggctaat
    52741 aaagaaggaa gggcgatctt gagcaaagaa agttcttcat tcccaacatg ctcgtcagcc
    52801 aaagcgttga gtaatgcata gaaacgattg attgcctcat gtttcaaata agctggatca
    52861 ataggctgtt ccaatatcgt cgtgaagaaa ttctcaagtt ctttgacagc taaagcgtag
    52921 tctttatgaa cgatttttgt taaataacta cttgcttgct gctcgtggtt ggttaataac
    52981 ggaagaagtt catgctgata agcaaggttc tttcccttat gatagaaacg ttgtcctttg
    53041 gtgcaatttc tgatctcttg gaatatttca ttgatatctt ggattgcaaa agaatcagaa
    53101 acaacaaaaa aatcacctgg aaaaacttct tgacgaaact tttgaacaaa cttcctaagt
    53161 gtatctttgg aatcagtatt gattacatag ccggattctt ccgaaaaggt agaaacagga
    53221 taaatagtaa acgaatcgtc gctatccatc aatgaatggg tgatcgactg tttttgttta
    53281 gagccttgtt ttgaactgaa tggccgtttt tcggtatata acagaaaaac aaggtgtgtt
    53341 caaaaagaag agggtcaagc aatggacgag tttgtccagc aatctgctgt gaaagattct
    53401 cgcagatctg ctcataacga ttggtgattg ggcttttgtt gggctttttt ttgatttttt
    53461 gtgaaacggc agttaaggaa tttagaaaaa aatcaggact caatcgaggc tttaaaagat
    53521 aatcagcgac gccttcttta aaagaatctt taaccatatc aaattcatca tagctagaga
    53581 ggataaggat ctgtgtttca ggttgttgct gttttaatag ccgcgataat tcgattccat
    53641 ttaattcagg catcattaca tctgtgacca cgatatcgat tgcatgtacc aaagaaaaat
    53701 caagcgcttt cagcggactg gaaaaagtcc ctattaattg aaacccgtgc tcagaccaat
    53761 tgatcaactg tttcattcca tctaaaatcg tctgttcatc atcgataaga attacgcgta
    53821 ttggcatttt tttacccgcc ttaagaatag tcttgtaatt taaaaatact tatttctagg
    53881 agaaaatgca aggatatttt tattttaaag aaacctcacc atttcgatct ttggtctaga
    53941 tgctaagatt attccttttg tcatagttga tttgtatttt tttattattt taggagaaat
    54001 ttttgaagta tctgaataat aaagaaaccg ttttcaaatt ttggtatgat gaatgtagca
    54061 aacgtaaaca actaatggat ggaaaaggag cggataaaaa tgaacatcag aaagttaatt
    54121 gctggagtcg tttgtttagc ggcagcatca ggtttcttaa caggatgcgg aagtgctagt
    54181 agtacagcag acaaagaaga aaaagtcact gtctgggcgt gggatgagac attcaatatc
    54241 aaagcagtga atgaagcaaa aaaagtttat gagaacgaag aaacagagat agaagttgtt
    54301 acgatgtctc aggatgatat cgttcagaaa ctgaacaccg ctttagcgag cgggaataca
    54361 gaaggattgc ccaatatcgt cttgattgaa gattatagga tacaaggcta tttgacttct
    54421 tatccagatg cattcgctaa tttgtctgct attgtaaagg aagatcattt tgcaccgtat
    54481 aaatttgcgg taaacaaagt aggagataaa atatacggtg ttcctttcga ttcgggcgta
    54541 acagcaaact tctatcgtac ggatctgatg gcagaagcag gttatacaga agaggatatg
    54601 gagaatctga agtgggacga gtatattcaa gtagcgcgag atgtcaaaga aaagacggga
    54661 aagaaaatcg ctgaagtcaa tccttctgac cttggtcgag taaggatgat tatgcaacag
    54721 gcaggagaat ggtatacatc tgaagatggg gagacagtca cgatccaaga caatgcatct
    54781 ttaaagtatg gtcttgaatt gtttgccact cttttgaaag aagatctagt cgaacaaacc
    54841 tctgactgga atgctggagt aactgcgata caatcaggag cagtagcttc tagcccaact
    54901 ggcgcttggt attcttcaac gatccaagga gcagaaaacc aatctggaaa atggaagatt
    54961 gcaccaatac ctgcacttcc agaaaatctg caaaaagcgc aagcttctaa ccttggcggc
    55021 gctggctggt atgtgatcaa aggagtagct ggagaagatt cagcgaaaga tttcctagaa
    55081 gcaacgtttg cttcgaatga agaactgatg ggcactcttg cgaaagaaat cggactagta
    55141 tctacgatgc ttagcgcgaa tgaacaagaa gcttatcaag aagcttctga attttacagt
    55201 ggtcaaaaga tctttgacga tttttcaaca tggacatctg aaataccaga agtgaattac
    55261 ggacatgaga cctatgcaat tgaagcagtc gtagctgaag ctcttcatcg aatcatcaat
    55321 ggagaagaga cagacaaggt actagcagat acacaaaaac aagtagaagc acaattggct
    55381 aattgatcgg tggtaaaaaa gtgaatttct tcgctttttc atcagacaaa cgaaaagaca
    55441 aaaaagatca cgtctgcctt acagatattg tctttcttgt cttttcatgt agataatcaa
    55501 tgatagaatt ggagggaaaa gaatgaagca gaaagattta ttcaaaaacg ggcatgcttt
    55561 tttaatcttg ccagtcattt tgatatcaat catggttttt attccgatgg ttttggcttt
    55621 gatcacgtct tttcaaagtg gtccccctgt caatatgaca tttaacgggt taggtaatta
    55681 tcagcggatg ttgacagatg caacttttaa aaaagctttt tttaatacat tcctgtattt
    55741 acttgtacaa gtaccgatca tgttgtttct agcacttttt atttccaacc ttttaaatga
    55801 taaaaagttg aaatataaag ggttgtttcg aaccgctatc tttcttccat gtatcacttc
    55861 gatggtcagt tattctctga tcatgaaaag tctattttct caatctggcc ttgtaaacaa
    55921 cttgttgttg ggcattggta tgattgacac accgattggc tggttgactc atccaatttg
    55981 ggcgagagtt ctgatcattc tttcgatcac ttggcgttgg actggataca atatgatttt
    56041 tttcctttca ggcatgcaaa atattgattc ggagatttat gaagcagcat ctattgacgg
    56101 tgcttctaaa tggcagcagt ttatcaaagt gacgatccca aatttaaaac caatcatttt
    56161 gtttacttcc attacttcga cgatcgggac attgcagctt tttgatgaag tacaaaatat
    56221 tactggcggg ggaccagcaa atgcaacgac caccctatct caatacatct ataatttgag
    56281 ttataaattc acgcctaatt ttggatatgc ggcagcagtt tcctttgtta ttgtttcctt
    56341 tattgtgata ttgtcgataa tccaaacgaa agtaggagga gaaagtcgat gaaaaatatg
    56401 gtagaaaaag aaacaatcga acgccccaac gtcaaaaaac aagcattaga acttcgttcg
    56461 acaattgggt cagtattaaa gtatacagca ttaagcatcc tatcatttat ttccattttc
    56521 ccttttgtct ggatggtttt gggaatgacg aatacacccg tggatatcac atcgggaaaa
    56581 ataaagattg gcagtaactt gatcatcaat tttcaaaatt tgttttccaa tgagctgaat
    56641 tttactcgtt ccctttggaa ttcagcgatg atcgctttgg tgaccactgt gtttgcattg
    56701 ctgatttcgt caatggcagg atatggattt gagatttatc aaagtaaacg aaaagaccta
    56761 attttcaata ttctactgct atcgatgatg gttccttttg cggcattgat gatcccttta
    56821 tatcgaatgt tttcacaatt gaacggaacg gttttgggaa tcaacagttt cttggttgtc
    56881 atcctgccat ctgttagcac tgcttttttg atcttcttct ttcgacaaaa tgttaaatct
    56941 tttccaaaag atctagtaga agccgctcga ttagacggtc taaaagaact aagtattttt
    57001 tttagagtgt atatgccgac ggctaaaaat acctatgccg cggctgcaat catcacattc
    57061 atgagtagct ggaacaatta tttatggccc ttagtagctt tgcaatctcc tgataaacgg
    57121 acggttccgc ttgttttatc tgctatgggt gcttcctata caccagacta tggaatgatc
    57181 atggctggat tagtcatcgc tactttacca actgccatcg tattttttgt tttgcaaaag
    57241 caattcgttc aaggaatgat cggctctgta aaataaaaaa acacaataag cgcgttcctt
    57301 caaagtcatt tttccaagta aaaagatact tttgggacgt gtgttatttt atgttgagaa
    57361 aaattaaatg ataacgtttt aagtttgtgc tcgaaaactc tttcatttta aggtaaactt
    57421 aaagagtaaa ggaggaggat acatgctaaa aaattttaaa gaagtgattg ctgctatatt
    57481 gccgatgact gtcctgattg tcattttaac gtttgttttt gcaccgttag aaggagaaga
    57541 cctcgcttca tttctggttg gtgcggcaat catgatgatc ggaatgacgc tctttttgtt
    57601 tggagcagat tattctatga tggaagtcgg agatctagta gggaaatata tgatcaaaaa
    57661 gaaaagtttg accgttttgg tcagtttggg atttgctatc gggatcgtga tcacgattgc
    57721 tgaaccttcc gtgcaagttt tgggacaaca agtatatgat atatctgacg gagagattgg
    57781 cagagtgttg ctgattggga tcgtcagcgt cggcactgga gtatttctag cctttgcgct
    57841 gcttcgggtc gtattcaagc tatcctatta tcaattgatg gctataggct acataggcgt
    57901 tttgatcgcc tcatttttca ccagttccga atttatgccg attgcttttg attcgggggg
    57961 agtgactact ggtccagtaa ctgttccatt tatcttggcc cttgccggag ggatgactag
    58021 tatgatcaaa caaaagaaaa atgaaaatga cagttttgga atggtcggta ttgcttcgtt
    58081 agggccgatt cttgccgtga tgattttggg ggtgatcttc caatgagttt ctggcagcaa
    58141 gtgatagatg gaatcgaaat cgttaccatg gaagtcattt tggcagtggc acctatcgta
    58201 atcctatttg ttttaatgaa tatcattagt tttcgtatgc gcaaaaagcg tttctttgac
    58261 attatgaaag gtttcttgat aacgactatt gggttgatcc ttttcctgca cggagtcaat
    58321 atggcttatg taccggtagg acagcattta ggcagtgcta ttgcgggatt gccttacaat
    58381 tatatactgg ttccccttgg ctttttaatg gggttcttag tagggtttgc tgagccggcg
    58441 attcatgtaa tgatcaaaca ggttgaagaa ttgagcgagg ggagaatccg atcgaaaatc
    58501 atgctgatgg taatttcaat tgggatcggc gcagcagtag gattgtctat gtggcgtttg
    58561 ctcgtcggat tctctcttta ttatttcttg atcccaggat acgggctagc atttatttta
    58621 ggacgaaaag tggacaagat ctttttagca atggcttttg ataacggtgg agtagcaacg
    58681 ggcccgatgt gttcgacatt tattttatca atgagcgtgg caatcgctgc acaaattgaa
    58741 ggaagagatc cgatcattga cgggtttggt gtggttgcaa tgattgcatt gacaccaata
    58801 ttatccactt tagtactagg atatatttat aaacgaaaag atgaaaaaga cagaatcgaa
    58861 aaagaaagat tgcaaaaatt gggggaaagc aaatgaaaga tcttgtagca ttgaatcttc
    58921 acatgattgt gacgatcgtt gacggaggaa tgggcgaaga tgttatcgac ttttcaaaag
    58981 aagcaggtgc agcaggcgga acgatcttac atggaagagg ttctggtgta catgattcag
    59041 ggaaattttt aggtttggac atttcgccag aaaaagatat tgtgttgaca ttagtacccg
    59101 atagtttgac gaataaagta atggatgcga ttggtcaagg catcaacatc gatattccag
    59161 gtaatggtat ttgttttagt attgatgtag acaaagtcat tggtatcact aagattcatc
    59221 aatatcgaga aattatcgga atgcaagaac ttttagatga aaataacgat agagaaaagt
    59281 ctgaatcata gacaagcgtt tgaagaggaa ataaacacaa acaagaaggc ggatgagcgt
    59341 tatttccgct tttttgtttg tatttttctc gtgaatactt atgaaatagt aaatttttat
    59401 gaaattctac tctaagcatc agactttctt ttcaaaatat gctattttaa aaatgatgaa
    59461 aaaaggggga gaaaaatgaa ttttttagtt gttttaaaag atactctgat tgagcgatct
    59521 acatttctat atgttcaatt gcaggaaaat aaaacattgc tgcatttttc accagagttc
    59581 catctagagg ggctaacatt aggagaaatc tatcaagcag aagggctgtc ggctgttttg
    59641 cactttttta aggtagaatt ggaattacca gtaaaagatt atatcgagct ttctcttgaa
    59701 tcttgggatc gagcagtggt tgaactgttt ccagaaggtt taatgatcga tacaaacgat
    59761 gggaagatcc atctgactgc agcagaactg caaaaacaaa tgcgttatca aatagatgac
    59821 gaaaattctt tttctatttt tagacgtcag caaaagattc taaaaagctt tttaaaagtg
    59881 atcagtaaaa aaacggaatc tgatcaaaac gacacagctg ttgaaaaaat atccaggtct
    59941 gcttcacacg tctattcaat ttcctcaatt gattacgatg atccatcgtt ttatacgtat
    60001 gcaacagcta ccatctaaga agctgtttct tccattgacg gacacatttc gagtagtcaa
    60061 cagagaagat aaaaagaaag tgattgtgat tgactttacc agaaatcgga atgtccttca
    60121 ccaagttgca atggaaaagg ccaattaagg ctttttcttt ttggattttt ttgtcataat
    60181 aaatattaga tgatggagga gataatatga tgcaaacatg gttagaaagg ctaatacgat
    60241 ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc gaaatttgat cagttgaatc
    60301 gcggtctatt gatcgctgcg cttgtcttta gtttgctaag tcgttggttg ccctatagaa
    60361 tcggacaatt gttgttcatc gtctttttcg gatggatgat atttcgtttc ctatcaaaaa
    60421 aaatctaccc cagactaaat gagaatcaac gttatttgaa atatcttgat agagtcaaag
    60481 aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat ggatcgaaaa agttatactt
    60541 tttttgagtg cccgaattgt catcaaaagc agcgtgcacc taaaggaaaa ggacgaatac
    60601 gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat
    60661 gaaattagta ttattacgtc atggcgaaag cgaagcaaat tttgaaaatt actggacagg
    60721 atggctagat gtcgccttaa cagaaaaagg aaaagaacaa gcagcagttg ccggtcaaaa
    60781 aatgcttgaa gccggacttc aattcgatgc cgtttatacc tctgttttga aacgtgcgat
    60841 tcttaccgga caagtcgcac tagaagaaat gggtcagtta tggcttccta taataaaaac
    60901 atggcgtcta aatgaacggc attatgggga tcttgtgggt aaaaacaaag ataagatgaa
    60961 agaaatatac ggaaaagatc aagtaaaaac atggcgcaga ggatatacgg aagttccgcc
    61021 gttaaccgca gaaaatagat ttgaccgcag atatgatcac ttggatccgc atttgatccc
    61081 atacggagaa agtctggaaa tgacagtgaa tcggatcatt cccttatggc aagatcatct
    61141 agcaccaaaa ctaaaagatc ggcaagattt actagttgtg ggtcatggta atagcatccg
    61201 agcactgaca aaatatttag aagatgttcc tgaagatcaa atggatacga tcgatatccc
    61261 aaatgcacaa ccaatccagt atacattagc agatgatttg acaattctgg acaaaaaaat
    61321 actttaaaga acacctttat ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa
    61381 caaaaggaac atgaaaaatt atatatggga ctttgatggt acattgtttg atacgtatcc
    61441 cgcaatggta gatggtgcat ggcaagcact aaaagatttt ggtatcagta tggacaaaaa
    61501 agaaatctac ttcaaaatga aaaagtattc tactagttat ttgatcaatg aatcaaactt
    61561 gaatgccaga gagtttaatg aattatttca ccgatatgaa aaagaaagta cggaagtttc
    61621 ccgtccattt ccagaaacga aacaagtact ggaaatgcta aaagacaacg gcggtcgtca
    61681 tttcatcctg acccaccgtt tgacagaatc aacatggggc ctgttaaaag aacatcgatt
    61741 ggcacatctg attgaggaag tagtagggat cgatcaggac tttcctcgta aacctgatcc
    61801 agcatcttta aattatttga tcgatacttt tcatttagaa agaacagaca cgatgatgat
    61861 cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca ggagtcgcta cttgtcttta
    61921 cgacatcgat cattttctgg gtgagatccc ggcagattat gtggtaggaa atctgaatga
    61981 aatccttacc ttgcagagca tcattaaaaa gtagtatccc ctttcaaagg agatatgtta
    62041 taattttcat aaaggagtga taaaattata tggaaatcag aagacaaaaa gaagttgtag
    62101 aagcatttca ttatgatatg agattgccga accaagaagt agaaacagat ttgaaagtag
    62161 gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa agaacatagc atcgtaggag
    62221 cacgcttaga gttccgttta gtgtttgata catatgtttt atctggaagt gtgagtcaga
    62281 tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga tgtcacacaa gaagaagtgg
    62341 acgagctagt agcaccgtta ttcgatatcg tacgccgaat gtcttatgag gtgacagaga
    62401 tcgccttaga tcagcctggg gtaaaattga attttcaatc agaaaaacaa gaatagacag
    62461 taaaaacata cctgcgatag attaaagcta tcacaggtat gtttttttat tactaattgt
    62521 aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat ataaaagtca ctgaaaaaat
    62581 ttttttcagg aggatggttg aagaatcatt ctcgggaacc tttacttcct tcgatggact
    62641 tgtcattaat aatatgagga ggagaaaaat gacaacactc atcacagttt tagtggttct
    62701 agtttttgcg gcattattat atgtgttcta tcgtatgcaa aagcgccata caaaattttc
    62761 aacacgcgtc tttacagcat taggagttgg tatcgtcttt ggcggtctgc ttcaattgat
    62821 ctttggtgta gacgacaaag tcgttactca atcaatggaa tggatcggga ttgtaggtca
    62881 aggatatgta tcattcttgc aaatgctagt tatgccttta gtgtttattt cgatcattgg
    62941 tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa attagtgcga ctgtattgac
    63001 ttctttactt ggaacaacag ctattgcagc attgatcgga attggttcaa caatgctgtt
    63061 cggactccaa ggagctaaat tcacacaagg agctgcagaa acagcacgaa tctctgaact
    63121 ggcaacacgt caggaaacag ttgaaaactt atcgattcca cagcagattt tatcctttat
    63181 ccctaaaaac gtttttgctg atttagcagg tagtcgtgca acaagtacga ttggagtcgt
    63241 tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg aaaagaaaag cgccaaaaga
    63301 agcagcattc ttttcagatt tagtcgacag tctgtataaa atcattatgc ggatcgtgac
    63361 gcttgttttg cgtttgacac cttatggagt atttgctttg atgacaaacg ttttggcaac
    63421 aagtgatttt gcagctatcg tcaatttagg taaatttgtc gttgcttctt acgtagcatt
    63481 gcttgctgtt ttgattgtcc actcactgat tttgattggt gcaaaagtca atccaatcaa
    63541 ctatttcaaa aaagcctttc cggtattgag ctttgcgttt acttcacgtt caagtgctgg
    63601 agctttgcca ttgaatatcg agactcagac aaaagcttta ggagtagatg atgcaacagc
    63661 taatttctca gcaagttttg gtttgtctat tggacaaaac ggctgtgctg gcgtttatcc
    63721 agctatgttg gcaacgatcg tcgcaccagc agctggagtg aatgtcttta gtttggaatt
    63781 tattttgatg cttgtggcaa ttgtaacaat cagttctttt ggtgtagctg gagtcggtgg
    63841 gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg aacttgccag tagcaatcgt
    63901 tgggttagtt atctctgttg aaccattaat cgatatggca cgtacagcag taaacgtcag
    63961 cggtagcatg gtagctggta tcgtgacaag tgcacgcatc aaagatttag atcgcgatgt
    64021 catcaatgat gattctaaag tgatcgatgt gaatgcatag tcaaaaagag gttgtgatag
    64081 aagtggtcag ctttaagaaa taagaagaag tttcctaaaa atagtttctc aaatttttgt
    64141 gaaatttcgg cttatttccg agaagctact tctgaaacac cgtgaataag aggttgtgaa
    64201 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    64261 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    64321 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    64381 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    64441 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
    64501 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    64561 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    64621 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    64681 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    64741 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    64801 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    64861 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    64921 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    64981 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    65041 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    65101 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    65161 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    65221 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    65281 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    65341 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    65401 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    65461 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    65521 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    65581 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    65641 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    65701 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    65761 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    65821 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    65881 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    65941 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    66001 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    66061 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    66121 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    66181 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    66241 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    66301 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    66361 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    66421 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    66481 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    66541 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    66601 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    66661 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    66721 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    66781 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    66841 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    66901 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    66961 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    67021 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    67081 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    67141 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    67201 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    67261 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    67321 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    67381 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    67441 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    67501 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    67561 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    67621 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    67681 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    67741 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    67801 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    67861 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    67921 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    67981 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    68041 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    68101 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    68161 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    68221 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    68281 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    68341 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    68401 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    68461 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    68521 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    68581 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    68641 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    68701 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    68761 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    68821 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    68881 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    68941 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    69001 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    69061 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    69121 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    69181 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    69241 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    69301 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    69361 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    69421 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    69481 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    69541 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    69601 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    69661 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    69721 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    69781 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    69841 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    69901 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    69961 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    70021 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    70081 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    70141 cagacctaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    70201 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    70261 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    70321 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    70381 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    70441 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    70501 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    70561 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    70621 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    70681 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    70741 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    70801 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    70861 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    70921 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tttaaccata gtataataaa
    70981 taatagtagt taaagtagtt taagaggaga tattaaaggg atattttact ttaaataaaa
    71041 attaacaagg tttataattt gtagtagtta aagtatttaa agaggtggga acatgtgaag
    71101 gttcaaagga tagaagtgga gaataagccg tatccattgt atttattact agataaagaa
    71161 taccagctaa tagaaccagt aatgaaattt attaaatact tagataatac tggtaagtct
    71221 cctaatacca ttaaagcata ctgctatcat ttaaagttgc tgtacgagtt catggaacag
    71281 agaggtgtta ttcttaatga tattaacttt gagttgttag cagacttcgt aggttggttg
    71341 agatatcctt cagcatcaaa tgtaattgat cttcagtcaa aaaaagccat aagagaagaa
    71401 acgacagtga atacaatttt aaatgtagtt atgagttttc ttgattattt aagtagatta
    71461 ggagaattta aatcaattga tgtatttaaa caagcaaagg gaagaaattt caaaggattt
    71521 ttacatcatg ttaataaggg tagataccaa aagaatgtct taaagttaag ggttaaaaag
    71581 aaacagataa gaacattgag atcagaggaa gttaagcaaa ttattgatgc ttgtcatacg
    71641 aaaagagata aattaatttt aatgcttatg tatgagggtg gtttaagaat cggtgaagtg
    71701 ttatcgctta ggcttgaaga tattgtcact tgggacaatc aaatccattt aacacctaga
    71761 gatgttaatg ttaatgaagc ttatattaaa ttaaggaagg aaagaacaat acatgtgagt
    71821 aaagaactta tgtcacttta tacagattac ttgatatatg agtatagtga ggaattggag
    71881 catgattatg tttttatttc cttaaaagaa ggctattttg ggaaaccact aaagtaccaa
    71941 agtgttcttg atctagttag aagaatagtt aaaaggactg gaatagaatt tacatcacat
    72001 atgcttcgcc acactcacgc aacgcagcta attagggaag gatgggatgt tgcgttcgtt
    72061 caaaagagat taggtcacgc acatgttcag acaacgttaa atacctatgt tcatctttca
    72121 gatcaggata tgaaaaatga gtttaataaa tacctcgaga gaaaggagca taagaaatga
    72181 atgcttctag taaaaggaaa attattagtc agagtgagat tagcaaaaaa atagctgtaa
    72241 tgaatgaaga aatgcagggg ttttgggcta ataatagttg ggatataaga aaatgtccac
    72301 atccttctgc catagaatta agtaagaatc ctgctttaag gaatcgttgg gttcgttttg
    72361 aacgtgttaa aaatctgtgg ttaagaacag aattgaaata tttttatttt taccatttaa
    72421 acaatggaat atggaatgca aaaactgtct ggattagaaa aggaacagta attaataaaa
    72481 tgttagattt cttagattta aagtatccta gcattacttc aattactgaa gttcctattg
    72541 aaaaagcaat gacggagtat agaacttatt tgacaaaacg gggtgttaga attaccacta
    72601 ctaattataa gattactgct aatcaagaaa aaacacctgt aaaagctaat tcctactatg
    72661 ttactaatct aaaacaattt atggagtttt atgagaactt ttattttgat ggagaggagt
    72721 gggataaaga cgtttgggat agacgtaact tacctttgcc agatgataag gttaacccaa
    72781 cacaatatga atatacaatt aactttaaag ggtttcggaa tacatatttt aaacaacttg
    72841 taaaaagata ttgtaagttg agattgaaca tggatagctt ttcctatgta agtgatattg
    72901 cccaaagact taaagagttc tttaattttc tggacatgaa atttaaacaa gttcagagag
    72961 tacaccaatt aacgagagtg gaaattgaag catatttaag tgaactaaac atgatgggaa
    73021 taaaacctag tacaataact gggaggatct ctatattgga aggactattt agtacccttc
    73081 ttaggctaga atgggatgat gttccttcca aaatattaat ttattctgag gactatccga
    73141 aaataccaag agcaaaacca cgctttatag atgaattcgt cctagagcaa ttgaacagtc
    73201 atcttgataa attacccgaa tatatagcta cgatgactat gattgttcaa gaatgtggaa
    73261 tgaggataag tgaattgtgc accttgaaaa aaggctgtct attagaggac aaagatggag
    73321 atttcttttt aaagtattat caatggaaaa tgaaaaagga gcatatagtt ccaatatcta
    73381 aagaggtagc tttacttatt aaagttcggg aagataaagt ttcagaggaa tttccagata
    73441 gtgaatacct ctttccaaga aaagatggat cgccattaaa acaagaaaca tttagaggtg
    73501 agttaaataa attagcttat gagcaaaata tagtggataa atcaggtgag atttatagat
    73561 tccatgccca tgcctttcgc catacagtag gaacaagaat gattaacaac gggatgcccc
    73621 agcatattgt gcagaaattt ttggggcatg aaagcccaga aatgacaagc agatacgctc
    73681 atatctttga tgaaactcta aaaaatgaat ttactaaatt tcaggaaaaa ctggttacca
    73741 ataatggaga tgtgcttgat ctagatgaag ataatgaagt cgatgatgta gagcttcaat
    73801 ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg ttattgtaga ttgccagtag
    73861 tagcaggtgg ttgtccacat gcgaatgcat gcttagattg cactcacttc tgtaccagta
    73921 agcaattctt accacagcac gaagaacagt tagagcgtac agaagagtta ttagccatag
    73981 ctaaggataa acaatggcaa agacaagtag agactaatag ccgtgttaaa gagcgtttag
    74041 aacaaatcat tggaagtttg acggggtaat tatcaatgga taaacaagtt agaaatacaa
    74101 cagaaattgt acgtttggcg aagcagaaat caaaaaagac aagggaaaaa gtagacaaag
    74161 cgatttctaa attttcgatt gaaggtaaag ttattaattt taattcaata gcaaaggaag
    74221 ctaatgtttc taaatcatgg ctttataagg aacacgatat taggcaaaga atcgaatccc
    74281 ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt tctcgttcgg
    74341 aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga attagaaaaa gaaaataaaa
    74401 aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagaa taattattaa
    74461 tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt attgaacagt
    74521 catttaggta tatgtccaat ctagggtaag taaattgagt atcaatataa actttatatg
    74581 aacataatca gttacgaggt gaaatcatga gcaatttgat taacggaaaa ataccaaatc
    74641 aagcgattca aacattaaaa atcgtaaaag atttatttgg aagttcaata gttggagtat
    74701 atctatttgg ttcagcagta aatggtggtt tacgcattaa cagcgatgta gatgttctag
    74761 tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa actaacagaa agactaatga
    74821 ctatatcagg aaagattgga aatacggatt ctgttagacc acttgaagtt acggttataa
    74881 ataggagtga agttgtccct tggcaatatc ctccaaaaag agaatttata tacggtgagt
    74941 ggctcagggg tgaatttgag aatggacaaa ttcaggaacc aagctatgat cctgatttgg
    75001 ctattgtttt agcacaagca agaaagaata gtatttctct atttggtcct gattcttcaa
    75061 gtatacttgt ctccgtacct ttgacagata ttcgaagagc aattaaggat tctttgccag
    75121 aactaattga ggggataaaa ggtgatgagc gtaatgtaat tttaacccta gctcgaatgt
    75181 ggcaaacagt gactactggt gaaattacct cgaaagatgt cgctgcagaa tgggctatac
    75241 ctcttttacc taaagagcat gtaactttac tggatatagc tagaaaaggc tatcggggag
    75301 agtgtgatga taagtgggaa ggactatatt caaaggtgaa agcactcgtt aagtatatga
    75361 aaaattctat agaaacttct ctcaattagg ctaattttat tgcaataaca ggtgcttact
    75421 tttaaaacta ctgatttatt gataaatatt gaacaatttt tgggaagaat aaagcgtcct
    75481 cttgtgaaat tagagaacgc tttattactt taatttagtg aaacaatttg taactattga
    75541 aaatagaaag aaattgttcc ttcgatagtt tattaatatt agtgacattt gcatgcttca
    75601 aagcctgtcg gaattggttt ttagtgaaaa gaacacgata ttcacggttt acccacttat
    75661 aaacaaaaga tcgatacttt ttgtagtcct tctttgaaat caatggttga tgtcgttcaa
    75721 gaacaatcaa tacagagtct acacttggct taggatgaaa atatagtggt ggtacttttt
    75781 tgagcatttt tatatccatc tccaccatta atagtaaacc caaagctcgt tgcagatttt
    75841 gcaatctttt cgcaaatccc ttctcaacga taagatagct atatttagcc tgactttcaa
    75901 aggtaattct tttgacaata tccgtactga tgttataagg aatattacca tatatcttat
    75961 agtttatatg ttttgggaag gaaaatttta gaatatccgt ttgaatcact tttatattct
    76021 cagaggggtt taccgcttct ttagtcactt gacataagcc tccatcaatt tctatagcag
    76081 taactgatcg actcattttg actagctctt tggtaaaatg tccttttcct gatccgattt
    76141 ctattacgtt gtcttgttta ctgatattcg tgtgattcaa tatttctttt acatgctttt
    76201 tagaagtaat aaaattttgc gtgtctttag ggtttttctg gttcattata accttctcct
    76261 tactggttat aatgaactga cattaagcgt tcattataac caattaattt tgatttggtt
    76321 gataacgaac tttattaata acaaatatag aaaaagtacc catactttta tctcctttcc
    76381 atttttttca ttatgaatga gataaagtaa tttctactac tgcgatactg gtgcacataa
    76441 ttgaaacctc cttatgtaac tgaatatgat tgtatcatct tttcatttta gaataaacct
    76501 ttatatgtaa ttttgcactt gacacttcag atggacgttt tcttcaaaat aatatgtttt
    76561 gaaacttgat aagagaagcc actgtcttta ctaaaaagta aaggctgcga taaaggacag
    76621 tgactttaat aaagttgcac tgttgtattg tctttggata aaattgtcta aagggaagtt
    76681 ttatccccaa cgaaaaaaca tctaaagtgg taagtaatga aagaatgtcg ataaagtagg
    76741 aatgggggat ctttttgaat acaatagaat cacagtttga taaggttgca gaagattacg
    76801 atttcgtgaa tgagcttttg aatgattatt cattctttgt gtctaatatg tctccaaaga
    76861 aaggcagagc attagatatc ggatgtggct cgggtttgtt agtggagaaa ttagcaagtt
    76921 attatgatga agtggtaggg attgatattt ctaatcaaat gctcgatctt gccaaatcta
    76981 aacgtcaact aacaaatacg gtctatctga atatgaatgc agaacaactt aattttaatg
    77041 agaagtttga ttttattgta agccgaacaa cctttcatca tttggatgat atagccagcg
    77101 taatacagca aatgaaggaa ctgttgaatg aggaaggaag aatagttatt cttgataatg
    77161 tatctgaagt cgagacaccg cctacctatg tttataaatt aggggcaatc caagagtttt
    77221 taccgcattg tttcaaattt ggaataaaaa atgcgattag aatttataat cacaacacat
    77281 ctaaatcatg gcttgagcat ttagcttcag ataagtacct atctgaacaa aattattacg
    77341 atttatatga aaagttgttg cctggatgcc aattccataa aatgggttgg gcaatggggg
    77401 ttgtctggac aaaataactt aaagctgtgg ctttgaaaag ttgaatttgt gattgttttc
    77461 agagctatcg ggtgctttag tataaataac aatgaataaa aaatcagcct gtatcttatg
    77521 ttactattaa gtaatttaag atacaggttt tactattttt gaaataagta ccttccactt
    77581 caaatacatg tattcaaagt gaaagtattg atttaaatga ttcttttgta atttactctt
    77641 gataatatga aattccatcg cagcaggata tgaattttac caaaaggata caaaagtgtg
    77701 gagaaatgat ggggatcact gtactggacc acttgatcat cggacgcaaa cgatatttta
    77761 gtttgagaga agaaggaatg atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa
    77821 gcaagtgtac aattatattg taattttgtt tgggaataga agttgaaaca tctgttgcac
    77881 ctctatcgta cacgagcaat gttacaattt aaattagtgc ttttggcacg aggaggatat
    77941 tattatggaa aacggaacag taaaatggtt caacgctgaa aaaggatttg gatttatctc
    78001 acgtgaagat ggaagcgacg tattcgttca cttctcagct atccaaggtg acggattcaa
    78061 aacattagaa gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc gcggcccaca
    78121 agctgcaaac gttgtaaaag cttaatttta tctgtaaatc aaattgatgg tagacatcaa
    78181 tttttacgag agaacaaagc gagtaacaaa aatacctgcc acaagagaca atttctcttg
    78241 tggcaggtat ttttttgtat gaaaaagtaa ataaatttaa gaggctatga aagagccacc
    78301 ttgtatcaag gaataagaac aaacaaaaat ggcttctcat gtttttcatt tgttttctct
    78361 tatattcagg acaaggtgct gatttcataa cctcttattt attcttcatc tggtgtcatt
    78421 accattggca aaatcattgg atgtctttct gtacgttcga ataagaaagg ctgaagcgcg
    78481 ccagtcatcg cttctttcaa tttatattct gaacagtcag gtccattcaa acattctctt
    78541 aaagcgtgga ataagattcg ctgagcttca ttgatcattt ctcctgattc tcgcatgtag
    78601 acaaatccac gagagagaat atctggacca gccaagattt cttttttctt gatatccact
    78661 gtcgcgacag ctaatacaag accttcttct gaaagcaggt gacggtcacg taaaacgaca
    78721 ttaccaatat caccgatccc gctaccatcg atatacacat ctccagccac aaaatgccca
    78781 gcaggacgcg cagaatcgct agttaacgct aaaacatcgc cattttccat gatgaagcag
    78841 ttttctgcag gaacacctac atcttgtgct agagaagcat gtactttcaa catgcggaat
    78901 tctccgtgga caggaataaa atattttggc ttcatcaaac gaagcattaa tttttgttct
    78961 tcttgtccac cgtgaccgga agtatggatg ttattgattt ttccgtgaat cacttctgca
    79021 ccagcttcag acagcaaatt gatcaaatga ttgacacttg ttgtattacc aggaataggt
    79081 gaacttgaaa agatcactgt atcgtctggc tggatttgga tttgacggtg agtaccattc
    79141 gcgatgcggc tcaacgcggc catcgcttct ccttgagaac ctgtacatag gatcatcgtt
    79201 tcattggcag gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc tggaactttg
    79261 atatatccta aacgttgacc gttgacgatg gcattttcca tgcttcgtcc gaaaacagcg
    79321 attttacgac cagttgccac agcggcatct gctgcttgct gcaaacggaa gatgttagaa
    79381 gcaaagctgg caaaaatgat acgtccttcg atttttgaaa agatcttcaa aatagaatgc
    79441 ccaatcgttt tttctgattt agtaaatgtt ggaatctcag catttgtact atcagataaa
    79501 aggcacaaga ctccttcttc cccgatacga gccattttat gcaagtttgc tggttcgcca
    79561 acaggagtga aatcgaattt gaagtctcca gtcgctacga tatttccaga aggtgttttc
    79621 accacgatcc ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct gacagaagtc
    79681 tttctaaaac ggatgatcgt atcttcattg atctcatgga gctctgcatc tcgaagcaag
    79741 ccatgctcat ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc atagatagga
    79801 atatttgctt gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg cccgtgagta
    79861 atcaccaagg cttttacttt gtgtaagttt tgtatgatgt agctgtagtc gggaatgaca
    79921 tagtcaattc caagtaaatc gtcttccggg aatttgatcc cggcatcaat caaaatgatt
    79981 tcatcttgga attgtacccc atacgtattt ttcccgattt cacctaatcc accaatagca
    80041 aagacgcctg tctcgttatt tttgatggaa agtttcatat taaaactcca ctaatgagaa
    80101 atcagcattt tctttttcgt actctagatg acttccttct agaagttgaa cgaattcaat
    80161 attgtatggg gtattttctt ccacacgttg gcgagcgtct acatcgcttt ttgcctcaat
    80221 gtaaagagat tttgtttctt cccgtttagg gttgcgtact tttgtttctt gataataaac
    80281 tttatagatc attgttttgt ctcctttatc tgcgtaaagc agtgatttag ctagttgatt
    80341 tttttagaca acacaaaacg aggggacaag ccacctggtt tttcatatgc tttgactaga
    80401 atactggatt cgagtcaaag agacaagcct cgtttttgct tatctgttcg gatatttttt
    80461 caaccgtacc ttctgtataa aaaatataca acaattaatt gttattctag cataaaacag
    80521 caaataagta tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc atcccgctat
    80581 aatcataatg acttttttat gagagaagtg ttctattaat aggctcgttg tgctatcatt
    80641 tttagtaatg aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa ctaatgtggc
    80701 attataccat gcgttataag aaattcttgt ttttcaattt catatgcgta tttggtttta
    80761 ttttgattga attaggactt cctacgattt tagcacgtat gatcgatgta ggaatccgca
    80821 atgatgattt tgattatgtg aagcaacaag ggatatttat gatcgttatt actgtaatcg
    80881 gtattatttt gaatatcttg ttaggttatt ttggttcgag gattacgact aatatcgtag
    80941 cagatattcg agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag gaatacgaaa
    81001 atctaggcgt atcttcattg atcactcgta caacaaacga tgcttatcag atcatgttgt
    81061 tcctgcaaaa tattttacgt atcggtttta tggcaccaat gatgttcatt gtcagtttgt
    81121 acatggtcat gcgtacaagt gtgacactca gtttgtatgt agtaggtgca ttacctttac
    81181 tattattggc agtcgtagcg attgctaagg tgtctgaacc gttatctaaa aagcaacaaa
    81241 aaaacttaga taaaatcaac agtatcttgc gggaaaatct ttcaggattg cgtgtgattc
    81301 gtgcattcgt caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat gagaattatg
    81361 cttctagttc aaaaagctta ttccgtttga tggctgttgc acaaccgggt ttttatttct
    81421 tatttaatat cgtaatggta ttgatcatct ggagcggggc agtgcaaatc gatgcaggcg
    81481 agcttcgtgt aggagatttg attgcgttta tcgagtatat attccatgcc ttgttctcat
    81541 tcatgttatt tgctagtgtg ttcatgatgt atccaagagc agctgtttca gctggacgta
    81601 tccaagaagc cctagatgcc aaaccttcga ttgtagaaga tccaaatggt atatctgata
    81661 caaaaactaa agggtatatc gaatttcaaa atgtaacatt tgcttatcca ggccattcgc
    81721 aagagccagt gatacgaaat gtcagtttta cagcctctcc aggagagacc gttgctttta
    81781 ttggaagtac cggtagcgga aaatctacgt tgatccaatt gatccctcgc ttttatgatg
    81841 tgagtgaagg aaagatcttg atcgatggag tagacgtgag agattatcgg ctaagcaagt
    81901 tgcgagataa gattgggttt attccgcaaa aagcgctgct atttactgga acgattgcag
    81961 ataatttgcg atacgggaaa gaagatgcta cgcaagaaga aatggaacgt gcagctgaga
    82021 ttgctcaagc ttcagaattc atttctcgaa aaccagatgg atttaatgaa catttatcag
    82081 aaggtggttc caatttttca ggtggacaaa aacaacgatt ggcaatagcc agagccatca
    82141 tacgccgtcc ggaaatctat atttttgatg acagcttttc tgcattagat tatcaaacgg
    82201 atgcgaagtt acgtgctcgt ctgaaaaaag aaacgacaga atctactgta ttgatcgtag
    82261 cacaacgtgt gggaacgatc atgcatgcag ataagatcat cgtattaaat gaaggagaag
    82321 tcgtcggtat ggggacgcat cgggagttgt tggaaaaatg cccgatttat tatgatattg
    82381 ccgcttctca attatcaaag gaggaattag catgaaaaaa accttttctt ccttaaaacg
    82441 tttagctcga tatatcagac cgtatcgtga gacttttctt ttagtgatcc tatttacagt
    82501 tctgacagtg gctttcaacg tggctttgcc atatgttaca ggacttccta ctacagaaat
    82561 cagccgtaat attgccaatg gcgaatcaat caatttttct tatatattca aatgtctcat
    82621 ttggatcaca gttgttggaa tagggtattg tatttcgcaa ttactttctg gtgttttgat
    82681 gacaaatgtc gttcaaagtg ccatgcatga tttgagaaga gacatagaag aaaaaatcaa
    82741 tcgtcttccg gtttcatatt tcgataaaaa ccagcaagga aatattttgt ctcgtgtgac
    82801 gaatgatgta gatgcagtca gcggtgcttt acaacaagct tttattggta tcgtaaatgc
    82861 gatattggga attacgttgg ctgcagcaat gatgttttat atccagccga tgatggcgct
    82921 gatctctatg atcatgattc caacatcgat ctggatctca aaacgagtga tcaatgcttc
    82981 gcagaaatat tttcaaagca tgcagaattc tcttggtgag ctaaacggct acgtccaaga
    83041 aaatatgaca ggttttagtg tattgaaagt atatggacgc gaaaaagaaa cattggaagg
    83101 attcaaaaaa gttaatcaca gcttgaaata ctttgggttt cgtgcagcat tcatttctgg
    83161 cttgatgatg cctctagttc aattaactgc ctatgctact tatattggta tggctgttct
    83221 aggaagcttt tatgttatta ctggggcgat cgttgtcggt caactacaag cattcatcca
    83281 atatatttgg caaatcagtc agccaatggg gaacgtgaca caattgtctt cagttttgca
    83341 aagtgcatca gcggcaacga aacgtgtatt cgagatttta gatgagccag aagaaaaaga
    83401 aaacaaagta gatgcttcac tgccagaaac gattttagga gatgtgacat tcgatcatgt
    83461 cgattttgca tatgatccga agaaaccatt aatccaagat ttgaattttg aagtgaaagc
    83521 tggtcaaacc gtagctgtgg tcggtccgac cggtgccggc aagacgacat tgatcaatct
    83581 attgatgcgt ttttatgacg tagacaaagg agcaataagg attgatggaa tcgacaccaa
    83641 atcaatgagt cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag atgcatggct
    83701 ttatcaagga acgattgcag ataatatccg cttcggaaag cttgatgcga cagattatga
    83761 agtagttgat gctgcgaaaa cagcaaacgt agatcatttt atccgaacga tgccagatgg
    83821 atatgatatg gccatcaatt ctgagggaga caatgtgtct ttaggacaaa aacagttgct
    83881 gacgattgcc cgagcagtgg tctccgatcc taaaatcttg attttagatg aagcaacaag
    83941 ttcagtagac acccgtttag aagcgttgat tcaaaaagca atggatcgcg tgatggaggg
    84001 acggacaagt ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag atctgattct
    84061 agtgatggat caaggtcaga tcattgaaaa aggaacgcat gagagtttat tggcacaagg
    84121 tggattttat gaaaaactgt ataacagcca atttgctgag gaagcagaat aagtcatata
    84181 agaataaaac agtttaaaac ttattttttg ttctctattc ttgataatgg taaagtaaag
    84241 ttatcaagaa aagtgaggac acggagatgg aacagttttt aaatgaaaaa attactgaag
    84301 ccaaaagaga agttgatcgt ttaacgcacg tctgtcagac gaagcctttt gattcagctg
    84361 atttttttga aaatgagttt caaatcgaac gcttggcagc acaaatagaa gtgtacgaat
    84421 taattttaaa aaaagtaata gaagaaaata tagatgtaca ataaaaacag gtaaaacgtc
    84481 agttaccaga tactgtaaat agacatctgg cctgagcagg ttttacctgt tttttttctt
    84541 atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta caaaaaatga
    84601 aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga ttggtgatgt
    84661 gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg acagtaattg
    84721 gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg tcgatcatga
    84781 tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc tacatggctg
    84841 tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc atgcggttat
    84901 tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag ttattcctcc
    84961 taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa aagaagaaat
    85021 acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc atgcgcaagg
    85081 aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga tggtcagttt
    85141 gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc ttcctttgaa
    85201 acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc atatgctttt
    85261 gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac ttcaagcggt
    85321 tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa aatatggcgt
    85381 gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg atggatcatg
    85441 atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag caagaagctt
    85501 cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac aaagttcatt
    85561 gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat ataaagatag
    85621 atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat cgaattagca
    85681 agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa acgtgagtag
    85741 atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat taaattcata
    85801 atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt tctaatacct
    85861 aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg caaattagtc
    85921 gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga acaacagatt
    85981 gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg tctatatcgg
    86041 catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg aattggaatt
    86101 gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg agatcttgca
    86161 aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg attgttatat
    86221 cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt tttatctgcc
    86281 aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga ttttatctcc
    86341 agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga ttttagagaa
    86401 ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga agactatgac
    86461 gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt ttgaagcatt
    86521 gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac aatagaactg
    86581 aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg gcacccaccc
    86641 agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg gtatcgaact
    86701 agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag aagtggaccg
    86761 gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca aaaaattgaa
    86821 tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat tgagagaagt
    86881 gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa ttaaaactga
    86941 tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa gtagagattg
    87001 gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa tcgcattact
    87061 ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga tccaactagc
    87121 agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt tcgaacatcc
    87181 agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg atgaagaaga
    87241 aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc cggagaaagc
    87301 tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat tagtgatcgt
    87361 cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac tatttggagg
    87421 agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg gtacagcaaa
    87481 aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga tcgaacctac
    87541 tcaacgttac tcagccgctg attttcagca ttcaggtcga caattgatta ccgagattgc
    87601 gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc aatcccttct
    87661 atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa aaaagtatga
    87721 aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag aaaaagaccc
    87781 ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag ctctggaagt
    87841 tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc aattgtatga
    87901 ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa tcaatcaaag
    87961 agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt atgagcttgg
    88021 agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt ttgaagggaa
    88081 agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat acgcgaaacg
    88141 tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta taagtgagcc
    88201 aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg aataaaatgg
    88261 aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac ttttcagagt
    88321 caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta ttcacaatta
    88381 cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa ttacaagaat
    88441 tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat gagctaactc
    88501 cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat cgcgtgcagc
    88561 ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg caggttgaac
    88621 ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca ttatctcgtc
    88681 ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca gaccgccgac
    88741 atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa gctcatcgag
    88801 agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt attggctaca
    88861 cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact tattcgaaag
    88921 atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct gaaggatttg
    88981 agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa ctgatcgatg
    89041 ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat gtagtcgatg
    89101 ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg gatgaactga
    89161 atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc gacccagcga
    89221 tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa gaaggtaaga
    89281 tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct tatacacttt
    89341 ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca atggttttgg
    89401 acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca aaaaccaatt
    89461 caaaatggtt aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DACECS010000003        88902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.003, whole genome shotgun sequence.
ACCESSION   DACECS010000003 DACECS010000000
VERSION     DACECS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.003"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(524..1078)
                     /locus_tag="IWO31_000179"
     CDS             complement(524..1078)
                     /locus_tag="IWO31_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0300631.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1204..3132)
                     /locus_tag="IWO31_000180"
     CDS             complement(1204..3132)
                     /locus_tag="IWO31_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0300632.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3438..4154)
                     /locus_tag="IWO31_000181"
     CDS             complement(3438..4154)
                     /locus_tag="IWO31_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0300633.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="IWO31_000182"
     CDS             complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="IWO31_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0300634.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="IWO31_000183"
     CDS             complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="IWO31_000183"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0300635.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7624..9084)
                     /locus_tag="IWO31_000184"
     CDS             complement(7624..9084)
                     /locus_tag="IWO31_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0300636.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9074..10369)
                     /locus_tag="IWO31_000185"
     CDS             complement(9074..10369)
                     /locus_tag="IWO31_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0300637.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10701..11378)
                     /locus_tag="IWO31_000186"
     CDS             complement(10701..11378)
                     /locus_tag="IWO31_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0300638.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11408..12499)
                     /locus_tag="IWO31_000187"
     CDS             complement(11408..12499)
                     /locus_tag="IWO31_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0300639.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12600..13034)
                     /locus_tag="IWO31_000188"
     CDS             complement(12600..13034)
                     /locus_tag="IWO31_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0300640.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="IWO31_000189"
     CDS             complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="IWO31_000189"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0300641.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15072..16967)
                     /locus_tag="IWO31_000190"
     CDS             complement(15072..16967)
                     /locus_tag="IWO31_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0300642.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17025..18800)
                     /locus_tag="IWO31_000191"
     CDS             complement(17025..18800)
                     /locus_tag="IWO31_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0300643.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18850..20346)
                     /locus_tag="IWO31_000192"
     CDS             complement(18850..20346)
                     /locus_tag="IWO31_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0300644.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20540..22162)
                     /locus_tag="IWO31_000193"
     CDS             complement(20540..22162)
                     /locus_tag="IWO31_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0300645.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22611..23924)
                     /locus_tag="IWO31_000194"
     CDS             complement(22611..23924)
                     /locus_tag="IWO31_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0300646.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24169..24393)
                     /locus_tag="IWO31_000195"
     CDS             complement(24169..24393)
                     /locus_tag="IWO31_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300647.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24413..24781)
                     /locus_tag="IWO31_000196"
     CDS             complement(24413..24781)
                     /locus_tag="IWO31_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0300648.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24979..25689)
                     /locus_tag="IWO31_000197"
     CDS             complement(24979..25689)
                     /locus_tag="IWO31_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300649.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25707..26204)
                     /locus_tag="IWO31_000198"
     CDS             complement(25707..26204)
                     /locus_tag="IWO31_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0300650.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26241..26693)
                     /locus_tag="IWO31_000199"
     CDS             complement(26241..26693)
                     /locus_tag="IWO31_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0300651.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26828..27433)
                     /locus_tag="IWO31_000200"
     CDS             complement(26828..27433)
                     /locus_tag="IWO31_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0300652.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27883..28503)
                     /locus_tag="IWO31_000201"
     CDS             complement(27883..28503)
                     /locus_tag="IWO31_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0300653.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28505..29374)
                     /locus_tag="IWO31_000202"
     CDS             complement(28505..29374)
                     /locus_tag="IWO31_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0300654.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29397..29804)
                     /locus_tag="IWO31_000203"
     CDS             complement(29397..29804)
                     /locus_tag="IWO31_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0300655.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29823..31112)
                     /locus_tag="IWO31_000204"
     CDS             complement(29823..31112)
                     /locus_tag="IWO31_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0300656.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(31139..31441)
                     /locus_tag="IWO31_000205"
     CDS             complement(31139..31441)
                     /locus_tag="IWO31_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0300657.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31466..33559)
                     /locus_tag="IWO31_000206"
     CDS             complement(31466..33559)
                     /locus_tag="IWO31_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0300658.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(34048..34812)
                     /locus_tag="IWO31_000207"
     CDS             complement(34048..34812)
                     /locus_tag="IWO31_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0300659.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34923..35792)
                     /gene="mdcB"
                     /locus_tag="IWO31_000208"
     CDS             complement(34923..35792)
                     /gene="mdcB"
                     /locus_tag="IWO31_000208"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0300660.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35817..36893)
                     /locus_tag="IWO31_000209"
     CDS             complement(35817..36893)
                     /locus_tag="IWO31_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0300661.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36917..37510)
                     /locus_tag="IWO31_000210"
     CDS             complement(36917..37510)
                     /locus_tag="IWO31_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0300662.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37514..39190)
                     /gene="mdcD"
                     /locus_tag="IWO31_000211"
     CDS             complement(37514..39190)
                     /gene="mdcD"
                     /locus_tag="IWO31_000211"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0300663.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(39180..39479)
                     /locus_tag="IWO31_000212"
     CDS             complement(39180..39479)
                     /locus_tag="IWO31_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0300664.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39581..41227)
                     /gene="mdcA"
                     /locus_tag="IWO31_000213"
     CDS             complement(39581..41227)
                     /gene="mdcA"
                     /locus_tag="IWO31_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0300665.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(41234..42145)
                     /locus_tag="IWO31_000214"
     CDS             complement(41234..42145)
                     /locus_tag="IWO31_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0300666.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42265..43122
                     /locus_tag="IWO31_000215"
     CDS             42265..43122
                     /locus_tag="IWO31_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0300667.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43309..43671)
                     /locus_tag="IWO31_000216"
     CDS             complement(43309..43671)
                     /locus_tag="IWO31_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300668.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            44017..45456
                     /locus_tag="IWO31_000217"
     CDS             44017..45456
                     /locus_tag="IWO31_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0300669.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45463..48015)
                     /locus_tag="IWO31_000218"
     CDS             complement(45463..48015)
                     /locus_tag="IWO31_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0300670.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(48129..49460)
                     /locus_tag="IWO31_000219"
     CDS             complement(48129..49460)
                     /locus_tag="IWO31_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0300671.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49426..50904)
                     /locus_tag="IWO31_000220"
     CDS             complement(49426..50904)
                     /locus_tag="IWO31_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0300672.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(51209..53044)
                     /gene="tdc"
                     /locus_tag="IWO31_000221"
     CDS             complement(51209..53044)
                     /gene="tdc"
                     /locus_tag="IWO31_000221"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0300673.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53514..54941
                     /locus_tag="IWO31_000222"
     CDS             53514..54941
                     /locus_tag="IWO31_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0300674.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(55033..55398)
                     /locus_tag="IWO31_000223"
     CDS             complement(55033..55398)
                     /locus_tag="IWO31_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0300675.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(55395..55760)
                     /locus_tag="IWO31_000224"
     CDS             complement(55395..55760)
                     /locus_tag="IWO31_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0300676.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55952..56200)
                     /locus_tag="IWO31_000225"
     CDS             complement(55952..56200)
                     /locus_tag="IWO31_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300677.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            56199..56327
                     /locus_tag="IWO31_000226"
     CDS             56199..56327
                     /locus_tag="IWO31_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0300678.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56384..58078
                     /locus_tag="IWO31_000227"
     CDS             56384..58078
                     /locus_tag="IWO31_000227"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0300679.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            58178..58351
                     /locus_tag="IWO31_000228"
     CDS             58178..58351
                     /locus_tag="IWO31_000228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300680.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            58729..59862
                     /locus_tag="IWO31_000229"
     CDS             58729..59862
                     /locus_tag="IWO31_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0300681.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            59893..60213
                     /locus_tag="IWO31_000230"
     CDS             59893..60213
                     /locus_tag="IWO31_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300682.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(60242..60706)
                     /locus_tag="IWO31_000231"
     CDS             complement(60242..60706)
                     /locus_tag="IWO31_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0300683.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(60838..62091)
                     /locus_tag="IWO31_000232"
     CDS             complement(60838..62091)
                     /locus_tag="IWO31_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0300684.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(62120..62437)
                     /locus_tag="IWO31_000233"
     CDS             complement(62120..62437)
                     /locus_tag="IWO31_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0300685.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(62463..62783)
                     /locus_tag="IWO31_000234"
     CDS             complement(62463..62783)
                     /locus_tag="IWO31_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0300686.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(63247..64662)
                     /locus_tag="IWO31_000235"
     CDS             complement(63247..64662)
                     /locus_tag="IWO31_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0300687.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(64679..65995)
                     /locus_tag="IWO31_000236"
     CDS             complement(64679..65995)
                     /locus_tag="IWO31_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0300688.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(66145..66843)
                     /locus_tag="IWO31_000237"
     CDS             complement(66145..66843)
                     /locus_tag="IWO31_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0300689.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(67132..68121)
                     /locus_tag="IWO31_000238"
     CDS             complement(67132..68121)
                     /locus_tag="IWO31_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0300690.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(68201..69034)
                     /gene="msrA"
                     /locus_tag="IWO31_000239"
     CDS             complement(68201..69034)
                     /gene="msrA"
                     /locus_tag="IWO31_000239"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0300691.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(69209..70843)
                     /locus_tag="IWO31_000240"
     CDS             complement(69209..70843)
                     /locus_tag="IWO31_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300692.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(70830..72677)
                     /locus_tag="IWO31_000241"
     CDS             complement(70830..72677)
                     /locus_tag="IWO31_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300693.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            72664..74301
                     /locus_tag="IWO31_000242"
     CDS             72664..74301
                     /locus_tag="IWO31_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0300694.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(74446..75186)
                     /gene="truA"
                     /locus_tag="IWO31_000243"
     CDS             complement(74446..75186)
                     /gene="truA"
                     /locus_tag="IWO31_000243"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0300695.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(75377..76012)
                     /locus_tag="IWO31_000244"
     CDS             complement(75377..76012)
                     /locus_tag="IWO31_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0300696.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(76159..77400)
                     /gene="sstT"
                     /locus_tag="IWO31_000245"
     CDS             complement(76159..77400)
                     /gene="sstT"
                     /locus_tag="IWO31_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0300697.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            77598..78596
                     /locus_tag="IWO31_000246"
     CDS             77598..78596
                     /locus_tag="IWO31_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300698.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            78609..80627
                     /locus_tag="IWO31_000247"
     CDS             78609..80627
                     /locus_tag="IWO31_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0300699.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(80687..81403)
                     /locus_tag="IWO31_000248"
     CDS             complement(80687..81403)
                     /locus_tag="IWO31_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0300700.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(81630..82172)
                     /locus_tag="IWO31_000249"
     CDS             complement(81630..82172)
                     /locus_tag="IWO31_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0300701.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(82177..82404)
                     /locus_tag="IWO31_000250"
     CDS             complement(82177..82404)
                     /locus_tag="IWO31_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0300702.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(82483..84363)
                     /locus_tag="IWO31_000251"
     CDS             complement(82483..84363)
                     /locus_tag="IWO31_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0300703.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(84552..85166)
                     /locus_tag="IWO31_000252"
     CDS             complement(84552..85166)
                     /locus_tag="IWO31_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0300704.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            85308..85868
                     /locus_tag="IWO31_000253"
     CDS             85308..85868
                     /locus_tag="IWO31_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0300705.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(86173..86487)
                     /locus_tag="IWO31_000254"
                     /pseudo
     CDS             complement(86173..86487)
                     /locus_tag="IWO31_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(86741..88376)
                     /locus_tag="IWO31_000255"
                     /pseudo
     CDS             complement(86741..88376)
                     /locus_tag="IWO31_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        26631 a        18336 c        14690 g        29245 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
      181 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
      241 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
      301 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
      361 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
      421 aacaacttct cctgtttttg gtttggttgg cttattatcg agaagtagct actgggagtc
      481 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
      541 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
      601 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
      661 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
      721 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
      781 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
      841 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
      901 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
      961 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
     1021 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
     1081 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
     1141 caatgatttc actgttttta tttttcttta cagccactga tattatgagc taatttgaga
     1201 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
     1261 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
     1321 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
     1381 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
     1441 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
     1501 tatccgatca ccttttgcaa agagctggcg ttctttttta ttctccttcc tatttgtaac
     1561 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
     1621 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
     1681 gctttctcct gtttctccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
     1741 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
     1801 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
     1861 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
     1921 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
     1981 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
     2041 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
     2101 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
     2161 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
     2221 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
     2281 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
     2341 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
     2401 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
     2461 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
     2521 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
     2581 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
     2641 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
     2701 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
     2761 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
     2821 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
     2881 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
     2941 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
     3001 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
     3061 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
     3121 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
     3181 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
     3241 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
     3301 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
     3361 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
     3421 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
     3481 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
     3541 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
     3601 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
     3661 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
     3721 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
     3781 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
     3841 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
     3901 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
     3961 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
     4021 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
     4081 acaccatctc caagcaaaac taggatcagt gtcccaataa actctcccat gatttgagtc
     4141 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
     4201 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
     4261 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
     4321 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
     4381 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
     4441 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
     4501 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
     4561 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
     4621 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
     4681 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
     4741 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
     4801 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
     4861 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
     4921 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
     4981 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
     5041 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
     5101 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
     5161 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
     5221 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
     5281 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
     5341 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
     5401 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
     5461 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
     5521 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
     5581 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
     5641 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
     5701 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
     5761 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
     5821 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
     5881 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
     5941 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
     6001 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
     6061 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
     6121 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
     6181 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
     6241 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
     6301 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
     6361 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
     6421 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
     6481 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
     6541 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
     6601 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
     6661 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
     6721 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
     6781 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
     6841 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
     6901 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
     6961 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
     7021 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
     7081 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
     7141 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
     7201 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
     7261 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
     7321 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
     7381 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
     7441 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
     7501 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
     7561 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
     7621 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
     7681 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
     7741 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
     7801 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
     7861 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
     7921 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
     7981 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
     8041 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
     8101 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
     8161 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
     8221 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
     8281 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
     8341 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
     8401 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
     8461 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
     8521 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
     8581 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
     8641 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
     8701 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
     8761 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
     8821 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
     8881 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
     8941 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
     9001 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
     9061 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
     9121 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
     9181 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
     9241 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
     9301 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
     9361 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
     9421 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
     9481 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
     9541 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
     9601 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
     9661 cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
     9721 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
     9781 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
     9841 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
     9901 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
     9961 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    10021 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    10081 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    10141 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    10201 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    10261 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    10321 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    10381 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    10441 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    10501 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    10561 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    10621 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    10681 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    10741 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    10801 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    10861 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    10921 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    10981 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    11041 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    11101 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    11161 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    11221 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    11281 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    11341 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    11401 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    11461 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    11521 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    11581 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    11641 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    11701 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    11761 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    11821 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    11881 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    11941 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    12001 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    12061 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    12121 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    12181 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    12241 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    12301 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    12361 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    12421 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    12481 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    12541 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    12601 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    12661 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    12721 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    12781 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    12841 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    12901 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    12961 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    13021 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    13081 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    13141 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    13201 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    13261 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    13321 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    13381 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    13441 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    13501 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    13561 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    13621 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    13681 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    13741 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    13801 taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt tcaaatagtc
    13861 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    13921 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    13981 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    14041 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    14101 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    14161 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    14221 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    14281 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    14341 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    14401 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    14461 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    14521 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    14581 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    14641 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    14701 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    14761 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    14821 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    14881 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    14941 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    15001 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    15061 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    15121 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    15181 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    15241 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    15301 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    15361 ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt tccgtccgtt
    15421 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    15481 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    15541 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    15601 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    15661 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    15721 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    15781 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    15841 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    15901 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    15961 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    16021 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    16081 tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac catcacgatg
    16141 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    16201 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    16261 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    16321 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    16381 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    16441 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    16501 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    16561 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    16621 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    16681 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    16741 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    16801 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    16861 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    16921 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    16981 cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    17041 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    17101 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    17161 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    17221 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    17281 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    17341 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    17401 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    17461 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    17521 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    17581 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    17641 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    17701 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    17761 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    17821 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    17881 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    17941 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    18001 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    18061 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    18121 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    18181 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    18241 ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    18301 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    18361 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    18421 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    18481 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    18541 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    18601 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    18661 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    18721 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    18781 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    18841 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    18901 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    18961 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    19021 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    19081 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    19141 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    19201 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    19261 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    19321 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    19381 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    19441 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    19501 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    19561 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    19621 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    19681 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    19741 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    19801 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    19861 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    19921 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    19981 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    20041 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    20101 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    20161 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    20221 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    20281 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    20341 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    20401 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    20461 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    20521 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    20581 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    20641 cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    20701 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    20761 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    20821 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    20881 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    20941 taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt tcctcttttg
    21001 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    21061 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    21121 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    21181 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    21241 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    21301 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    21361 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    21421 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    21481 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    21541 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    21601 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    21661 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    21721 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    21781 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    21841 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    21901 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    21961 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    22021 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    22081 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    22141 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    22201 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    22261 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    22321 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    22381 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg aactattttt
    22441 tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt tataaacggt
    22501 gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag aaactatttt
    22561 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    22621 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    22681 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    22741 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    22801 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    22861 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    22921 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    22981 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    23041 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    23101 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    23161 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    23221 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    23281 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ctatcccgat gaagatgcca
    23341 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    23401 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    23461 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    23521 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    23581 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    23641 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    23701 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    23761 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    23821 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    23881 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    23941 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    24001 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    24061 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    24121 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    24181 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    24241 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    24301 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    24361 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    24421 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    24481 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    24541 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    24601 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    24661 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    24721 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    24781 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    24841 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    24901 aggattgctt tcctccacaa acttttttta caaaaaaaaa aaacggcaat gaatgccgct
    24961 cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta gtgtgtgtct
    25021 aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga ttgcctgtgc
    25081 gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg cttgtgtttc
    25141 ttccgtcagc attgttcttt gggcttcacg ccagttgaga cagcgacaga tcgctccttg
    25201 ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg cacctaaagg
    25261 caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga tcttttcgat
    25321 atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga tatctacaag
    25381 cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag cttcaataga
    25441 agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact gagcaatgac
    25501 ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg ctgcctcatt
    25561 caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga taccatttac
    25621 ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat ccactattac
    25681 ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag atagtctgtt
    25741 tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc ttttttacga
    25801 taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc tgttttttgc
    25861 ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc ttgatgttta
    25921 aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat ttcctcagat
    25981 tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat actcactagg
    26041 aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc ataagcttgt
    26101 gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc tgtaaatgtt
    26161 tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg aatttcgtat
    26221 ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac aaaattccaa
    26281 tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt tgcttcttcg
    26341 tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat gatgaaagga
    26401 tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt ttctttatct
    26461 tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc agtcgttgta
    26521 cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt ttcgcaaata
    26581 cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc gatattgcta
    26641 atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc catttcatcc
    26701 cacctttatt ttgtttgtaa gactagttta cctctttttg ttgcaaatgc aactattttc
    26761 aataaaaaaa tcaagttctt ttttaataac tagtaaagta tattcttttc ttcttttacg
    26821 aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac tgtttcttcg
    26881 ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc atgttggaag
    26941 tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca aaaacaagtt
    27001 tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa actgctcgca
    27061 actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc acattttctt
    27121 caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc gtccgtttat
    27181 cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct acttttggtg
    27241 ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt tcttttccta
    27301 tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct ttatcaaata
    27361 catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg tattgagcca
    27421 acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt aatcaagtat
    27481 agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa taaaagaatg
    27541 ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata aatggcgttc
    27601 aaaagcttgc atctgcggta ataagcccca ccaaacgaaa aatatgacga gctattcttg
    27661 tctggctgtt cttattaatt gaggcaggac agctttttca caacctcccc actaaacggt
    27721 gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga gaagctgttt
    27781 ttttgaaatt gctccttgtg actcggagct gaacgacagg ctcacgacct ctcggtaaac
    27841 agcgttcaaa agccaatttt tcgatattaa gccatttctt tgttacttat tgtcattgat
    27901 ttctttttct aatttatcca atagtggagt gtaatccact tgagtatagt ctgttacatc
    27961 aaattcatat agtgttccta actggaattt gtcatatttt cggtcggcga tgatcttttt
    28021 gaatgcttct ttcgtaatat gattggtccc tttcgttcga tcttccaata cgtcaccata
    28081 gtgataatga tccatgatat agctcaaatg acgatctgat tcacgatcac gtttgtaccg
    28141 acgttgacat aacacttcaa agtctgctgt taggcgaatc gtgatcacct gatatccgta
    28201 tctatctgct aattcttgga gtcgtgcttt ttgcttatca ctaaaaggat actcggacac
    28261 aatcactttc tttcctgctt ccatatacaa atcaagtgct ttgtaataga aaggataaac
    28321 tttcgtcctt tcttgttccg cccgttcttc caaattgtta aacccgatgg attctgcata
    28381 gtattccttg atttcatcga gcgtgatggc atacatatct ggaaatcgtt cccgtagttt
    28441 attcataaga tatgtttttc ctgtaccagg actaccagcc agaagaatga aatacttttt
    28501 catctcattt atttcctaag ttaattggac tttccaagtt cataaacagt ccaatcttat
    28561 gcttgacaat ttcttttact gcttcatttg ctggaatcac attatggcga agagacttgt
    28621 tgatttctgt ttcactaaag gtttctttag ctactttcgt ccagttgact aaaagctccg
    28681 ttccaacatt gaatttgcgg atcccgttat tgatcaattc aggataatct tcttctttca
    28741 cacctgttcc tccatggata actaggggga catcaactac tgcatcgatt tctttcagca
    28801 agggaatgtt tacttctgtt tttgatttga attgtccatg attcgtaccg atagcaatag
    28861 ctaatgcatc cacgcctgta gcttctacaa atttcactgc gtcttccggt cttgtatata
    28921 ccttgtcatt ttcagcgaca tggattcctt cttctgtacc gccgattgtt ccaagttctc
    28981 cttctacgga aacaccgtgc gcatgggcat attctactac tgcttttgtc ttcaggatat
    29041 tttctttgaa aggcagatgg gacccatcat acatgacaga agtatatccg ctgtcgattg
    29101 cttctttgat atcatcaaag tctcgggcgt ggtctaagtg caagactgca tcaaccattt
    29161 cttcttctgc aatagctgtc attacattga ctaacacatg catgccgata tatttagcgg
    29221 tatcgacact tgtttgaata atgatcggcg aacccatttc ttttgctgcg cctagcatag
    29281 caggaagcat ttctaggtta tgcgtattaa aagctccaac tgtaaaattc aactcttctg
    29341 ctgtttttgt tacttctttc aatgttgtat acatctttta ttgccacctt atctttttat
    29401 ttgtttcctt tgatcgttac ttgttttgca atttgtcgca tctcatccat tttgcccatg
    29461 tagtaatcga tcccttgatc atcaccagca cgagcagcat ctgcacgctt ctggttatag
    29521 aggctcatca gttttttata cccatctccg atcgtctgtt tattttccaa actcttttct
    29581 aaatattgga tggcttgatc gttcacatac aattcagaaa atttaagtcc aacttgttca
    29641 taaagtttcg ctaatttatg cttatcttgg gtagtggaaa tctcttgttt caattgtgta
    29701 atctctgatt gaatcttttc ggtcgcttct ggcgataggg ctttttcttc ttttgccaga
    29761 acttttttcg gttctttttc ttttttctta aaaaatccaa acatcatttt ccctactttc
    29821 aattaaaatg ctttcaaaac tgggcttaag atccaggcat ataagagtgc tgcggctact
    29881 gtatctacat cggttgctgt agcattgatg aatcccatcg aattaaagat cgttactagc
    29941 aaagcgggca ataatgtaat aaagaaacca tgagcaatac cgccaatgat cgctcctcgg
    30001 cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc cggcaaagaa attggttagc
    30061 ataccaggta aaatcatcgc taagccgaac atgggcaaag taaacatggc aataatcgta
    30121 ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg ggctataagg aaagaaaact
    30181 ggacaatcta aggcagggat cgcatcagga actaatttca tagctatccc tcgaaatgcc
    30241 ggcacgattt cacctaacag tagacgtaca ccagctagca atacatacac acccactacg
    30301 aattggatgg cttgtaaaaa ggcaaacatg atatagtttt gatctcctga taattcagaa
    30361 gcgaatggtt cgcctgcaaa taaaacagtg atgatatata gcgggaccat cacgaccatg
    30421 acagacaaat aagtatcttg cagaaattcg aaatgtgtcg gcaacttgat atcttcgatc
    30481 gactttttat tttcgccttt ctcaccaaaa agcttagcaa caccggcttc aaataagtac
    30541 ccgatcgtac aaaaatgacc taaagcaata tcatttgatc cagtgatttt acgaatgatc
    30601 ggttgcgcta ctgccggcat agcaatggcg aaacaagctc cgataaaacc accgaccaag
    30661 atcaatacga tccctcgcag accggcaaaa tagccaaaga ctgtcgtcat cgttgccatc
    30721 cataaaattg cttgaccagt taaaaaaatg tacttccatt ttgtgaaacg agccaaaata
    30781 atattgaata caaaaatggc taagaacgtc agtgcaatat ctctacctaa acccaaatca
    30841 ttcattgctt gaccattgat agattcaata gaagggatga tcccttgcat atgaaatcct
    30901 tcagtaaaga ttttcccgaa ataagttaaa cttccaacga tgatgctcga accggcactc
    30961 aacacttgaa atcctaggag tgtctttaac gtgccagaga tgacttgtcc tgctgatttc
    31021 ttctgcagta gtagacccaa catagcaatc aatgcaatgg tgatcgatgc ctgagtcaaa
    31081 atattttcga tgataaaatt gataacgccc atgtttttac cctcttttct ttttgtattt
    31141 agataactcc cttatcttgc agaactgggg tgattttttc ttcaatttca ttttttgaaa
    31201 caatattttt caaatagatc atcgccgttt taggatcaac tgcgaacttt tcgaactgcg
    31261 aacgaaaatt ttctgctgtg atgatcacat ctggtttcat ggaaactgcg gaagaaatat
    31321 cacaatggtc cagtttggct tctactccta atttgttcaa aacatcctca gcagacatct
    31381 gtgctgcaaa actacttcct aaacctgcac cgcatacgaa taaaatgttc aatttgttca
    31441 tctttgttct cccccgtatc atttattatt tctcaactac tgaaaaaagc agttgtttga
    31501 aatcttcgat atttgtgcat tcaatcaatc gatccaactt ctcttcgttg ttgatcaatt
    31561 caattagttc tttcatgatg ttcagatgcg agtaggagtc tactgctgcc aaacaaaaaa
    31621 taatctttac tggatccatt tcaggattac cgaaatcgat tggttgctca atcgtagcta
    31681 cgctgactcc taatttattg accccatctt ctggtcgggc atgggctagt gccatgtgtt
    31741 tgccaatcac gatatacggt ccgtaatcat tcactgcacg aatcatcgct tcgacatagc
    31801 gctcttcgac aatgttttct ttaaccaatg gtctagcagc tagttcaatc gattcctccc
    31861 aagtagtggc tttttctttg atcatgatat tgctgtccgt caaaatatct tttaacatag
    31921 gctggatctc ccttttgttg atttctaaat tgtttgtttc gaataatttt tctaacttgc
    31981 catagatgat tcgatctact cggccaccac tttctttcat gacatctatt atttgagtga
    32041 ataactccgt cgattcatga ctgttaggct ctaaccgctg tagttcttca tttcttttca
    32101 aaaagtcttc tataaccaag atattcgtct cagttaaaat cggatcgaca actaaaagcg
    32161 gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag attgacatcc aatttatcta
    32221 ctaattcgat ttcttttgaa cttaaaactg cagtaatctc gatctgagga aacttttctt
    32281 tcagattctc tgctaacaga tttccagtag ccatcccatg gttacagaca acgactgatt
    32341 tataaacata attcaagtct cgcttgatta cactggcgat cgtcgaaaag tggatgacaa
    32401 gaaaagctat ctcgtcctcc agtatcttgc caccaattac ttcttcgatc gtaggaacaa
    32461 aatgagtgac tgcgcggtag attgggaaat aatttgttcg gatgttttct ttcaatggat
    32521 tcatgacttg gatatgattc ttgattctta caatcagtgc agccatgtgc ttatagagat
    32581 tttcgaataa agcttcttcc tttagatgga aaggtacgtg tgtctcgttc tctacaaatt
    32641 ggatcaattg gatcgttagt aattgagctt gtacccactc gattgaattg gagatatctc
    32701 tagcattgaa cgtctcaata gtgaaacgaa gataatttct ttctacttgc gggacattgt
    32761 ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga aaagctactg tcttttattt
    32821 ctgtattttt ccaattaatg gcagagataa attcatgttt cctgattcgc tggatccaga
    32881 tcaatgtaaa aagcaggatt tgttttttat aaatatcatc ttcttgtttc aacaacttat
    32941 tggaatagat ctcctgtatt tttttgattt ccaccaaagg aaaataatgg agaaagattt
    33001 tttgagccgg tgttaattgt gcatcttgat tcataaagaa gtcggtcatt cccagattct
    33061 gattgataaa atcatagatc atcgtacgga tagatcgttc tgaaccttta taaaccagtc
    33121 cttgctttcc ataactgacg atctctatcc cataactgac cagtgttgct cgtaaccgtc
    33181 tcatatcttc atctaatgta cttttagaga tttgaaattc ttcttctttt ttgtttagat
    33241 ggatcgggat tcggctgtaa gcaacggcta aaactagatc aaaaatacgt tcgcgccgat
    33301 tcaaaaagcc gtctttcaca tcttttattt cagtttctaa tcgagcttgc ttttcctttt
    33361 ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat ctccggaagc tttttactgc
    33421 tcaaccagat attcgcttca agaacttcct gacggatcgt ccgctgactg acattgaact
    33481 cacttgctag atgttttgct tttactggat attgggctgt catgattctg tgaagtattt
    33541 caattgttct tgttttcata gaaatgatct ccttctgatt tcaagtatat ggttccgctt
    33601 acataataaa aagtagatat ttctgccact tgaagtggaa agaaagtaag caaagcaaac
    33661 aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa agacatacgc ctattattac
    33721 aaaaattttg cccatctata caaaaaagag gttgtgaaaa agctgtcctg catcaagtaa
    33781 taagaacaac taaactaaaa tagtgcttcc tatttttcgt ttggttgttc ttattatcgc
    33841 agatgcaagc ttttaaacac catttagtgg agaggttgtg acagagatgt tcagccttga
    33901 gcgataagaa gcgattttga aaaatcgctt cccatatttt acaaaatcgt gccttattgc
    33961 cgataggcta tctctggcac actgtttatt cagaaaaagg agctgcgaca aggcttttgt
    34021 cacaactccg ttcataaata tttcctgtca atccagcata atcggctgaa taaaatcggc
    34081 ttgccatctt ttttctttct cagaaaaatc aaaataccat atctgagaaa aatactgtcg
    34141 ttgtgtctta cttcctgaga tcaatcgccc atcttttcga tcgactgaat aatcacagca
    34201 tctgaaatca attcggatgg caaaactatt ttcgtggatc tgtcgataat ttcctacttc
    34261 ctgaatagtt atttttttag tatgatttct tacaccgtct gctttgtttt tttgcagaac
    34321 tttctgatgt tcggcaaaca gcttgtctgt ataacagctt ctggcttttt caggagtttc
    34381 ttcttcccaa gcggattgga tcttcatgaa tatcttttgg atttccagaa actgtgcttt
    34441 cttttcttta gccgttcctg acatgcggga caatatctcc gctttcagcc ttttcttttg
    34501 tcttttattc ttgacgatag aaaaagcaga aacccctcct actgacaaaa cagctaatgc
    34561 aagaaatccc gtttgctcat aactagaagg acctgtacct ggaccataat acccataaga
    34621 atctctgccg taagaacgac tgccaaaata actgccccca tatgaacgac caccaaatga
    34681 acctctgccc gaacttcgac cgctagatgt acctccacca tgaccacccg cgacagcgaa
    34741 tccttgggtc ggcagagcaa acaaacatag gcccactaac aaaatgattg ctaaataatt
    34801 gatttttttc attgtcttcc tcgttatctt ttattacaaa aaactatttt tatgatagcc
    34861 ttgctaaaag gaagagtcaa taaccagatg cataaactgt ttttttagca attttgtctt
    34921 atttagtcat tgagtaacat cggacaaatg tatgtgtcgg cagtccataa ttcctctatc
    34981 ttcagcatga acaagcttgc agacaagaga tctgcgcttc ctccaggaga gatttggttt
    35041 tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg gtaaagcact ttgactagca
    35101 gcatgagcaa gtttttgcat ttccactaac gttttctgat cactgcgatg caatacgcat
    35161 gtgtcatcta gcgaactcat caaaactaac aacatatgca tttgtttctg tacatatgaa
    35221 tcattatcta gctccttagc tgcttggatt gcaagcctga tatgcggata ccctaatatc
    35281 gcttcttcat atgcaccatt tacgccatat ttttgcttca ccctatgacc atgagtggtt
    35341 tttttatgac gatattttgt atcaggaaaa gcagcaagct tgctgacatc atcaagtatt
    35401 ttcgataaag aaaaaggctg ttccatactg attttttgtg caatgacact gatcaatagc
    35461 cccattgccc aaatcgctcc tttatgggta ttgatgccat ttgtagcaat cagcattgcc
    35521 ttttccgctt ttcgcccaat ctcagcaatt ctttctctta actcctgatc aatagcatga
    35581 tgccatgcta tttgagccat ttcttcaaaa aaaggagtta gcgtcttagc agacttaaga
    35641 aataaagcat agtccatatc ggaatgtgca cccgcatcca gtgcatccac taatccaggt
    35701 tttggactta atgttacttc tgcgatcaat gcttcctcta ccaacgcagc gagttgctga
    35761 gtcactgctt tttttcgttc gtcatttatc atgacactca ctccttcttc ctactattac
    35821 ttgctgaaac cttttttgat agctacccaa atgatatgaa gtaaccagct taaagcaaat
    35881 gcacaacttg ccgtcattag ataagctaca agaatcatga ctcctgaagg taactgcagc
    35941 caactatctt taaacaataa taacagtact tggaaaacaa atacattggc taaatacgta
    36001 cgataagcat aaacagacaa ccatttgaac aatggcaatt ttggcgaatc gtttacaatc
    36061 attttggaag cgcctatgaa gacagcaaag atgatgacta aactgtataa actttggatc
    36121 gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg gatgtgcgag caatgaacgg
    36181 acagaaaaga acgcgataac taaggcaaca ggtaagaaaa gataacgtac tctgtccaaa
    36241 tattgataga ctttttcgtg ataaacaaat gcggctaccc cataaatcat gaaactagca
    36301 acaaaccgat ctaataagta ccagctgtca tgataaggcc cagtcaatac ttgtgtttga
    36361 tagaacagat accatgcgac atataaaatc gttgaaccaa tcaatactgg ccatacatat
    36421 tttttattag caaatacttt gtaaccgagc caaacaaaaa acggcatgaa caattggatc
    36481 tgcagcatca taacggtata ccataaatga ggcgctccat cacctgtgac atatttcagt
    36541 aaaaagcttg cagtatccgt atacggagtt tgttgttgga ctgctggaaa tagccaaaga
    36601 taagccgtcg tccatagaag atacggaacg actagttcaa aaaacttttc tttcaaaaat
    36661 tcccaataag acaaatgctg acttgttttt accatattat aaacaattgc aaaaataaac
    36721 attggtgctg tgtatttaac gatgacgtaa aaggtaccaa gaaaagctgt atttgttgcg
    36781 ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca tcataacagc cgttacagct
    36841 gatactttct ggaagtcact tacgttgatt ttgatttttt tgttttcaaa catctctttc
    36901 tctctctttt tctttattac caaagttctt tggttaattt aggtccttcg actgttttca
    36961 atagaatttt tttctctggc tctctcaaat actcttttag tgaaaaaccg ccttgccgag
    37021 tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg cgattgaatt acctgaattg
    37081 gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa atcactggtt tcttttactg
    37141 ccggaatccc agtcgataat tcaaatccta agctgccgcc gattccccat ttacatcctg
    37201 ataatatgtt tctagcagca tgaaattgct gataaacagg ataatccatg attttctctt
    37261 gtctatacga ttcttgttcg atgatgtccc acggatgaat gacttgtttg acggcatgtt
    37321 ttgagatttc tgttgaccat ctctgattgc gggcaggtcc acgtaaacca atcggtagta
    37381 tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa catcttttca gttagaacga
    37441 cccattcagg caaggtgaac ccttctattt catgtttatc aaaaaggaca atatcatgtg
    37501 cttgaatcat tttttatgca tcccattctt cgtccatttt ggcacggact ttatttgttt
    37561 cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg atttgtcagc atcgtgtcat
    37621 ttgtattgcg gacatcttga atggcttcat tgattgcttc gatgactttg ctgacatttt
    37681 gttctgaagc agcttggtcg gtgatatctt ctaggaaatg atagatcgct cctagttttg
    37741 tataattatt gatatcgtaa gcgatagctg gaactttttc agcagctgct tcgatttcag
    37801 caaccgaacg ttttgtcaca cgtgcagcac tttctttaga catcgcttgg acggtaattt
    37861 ctttattgtt taaagcaatc aagcggcttg attgtaatcc atgtgctaaa aatgcacctg
    37921 aaatagcatt tcctgcaatc aatccaataa ctggatgtcc agcttgacgg agttttgcat
    37981 aagcagcagc actattagcc aaagccacat ggataccgat caattcttcg ttatagccgt
    38041 atgcttggct tggtacatcg atcacaacga tgattggccg tttcactttt ttgtctttat
    38101 cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa accttcaact aaaccaactt
    38161 caccgtttcg tacacggtac atcgggtttt taggatcagg tacgattgat gtcacgatgg
    38221 cttcttctat gaatttactt gatttaccat aatacacagt actaatagaa gaagttggat
    38281 ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac acttgctgca gctggttcat
    38341 ttccttctgc tacttctggt aagtcaattg attttgcttg atggttagca attgcttcat
    38401 tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga atcaagtaat gataaataaa
    38461 atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc cattgctgcg atgacagcag
    38521 ctttgattgc ttctacacta tcagtcacta attcatcaat gatttttgat tctacccgtt
    38581 gacgagatcc taatgtattc caaatcaagt ctttgtcact agaatcaaat tctcgaacgc
    38641 ctgcttcttg ttcaataact tctggtccat tcaaaccgac tctcgctttt tttgttgcga
    38701 ttaaataact cattaatgct gaagtaatcg acataccgcc aaatgatcct acacgtcctg
    38761 gaatcaaacc gatgactggt acatattgtt ttagagcaat gattacattt ccgatttctg
    38821 aaatagataa cagtccgtag tttgcttctt gcaaacgaac accacctgta tctagtacaa
    38881 tgactggaaa gatctcattt ccttttttat tttcttctaa cacatgttca agtgcagcaa
    38941 cgatttttgc accggaaact tcaccgatac cgccaccttg gaattttcct tccatagcga
    39001 ttactactgc ttgtttgcca ttgatcgtgc cacggctgac aacgatcccg tcatcacttt
    39061 cagggacgat tccttgtgct ggtaaacttg gcgcgatgat ttgatcgaat ggatcaatca
    39121 attcacgtcc attgtcaagt agagcgttca ctcgttctct ggcgtttaat tctacaaaac
    39181 tatttttcat ctgaaagttc ctccattgct tgcgtcaatc gtaggtacac aactcctggt
    39241 gtagcaccga agtcattgat cacgatatct gctgaaattg gataacgatc aaaaaatctc
    39301 gttaatacat tgttccatac ttcgccgaag ccatcgcttc ctgtacaaat ttttacagta
    39361 gattctgttt tttctgttgg aaacatcaag atttccaaat ctcctgaacc gaccactcct
    39421 acatggattt tcttattgat cggctgtttc gttggatatt tataagctaa ttgttccatg
    39481 gatctctccc ctttttgtaa aacagagcat acacgttctg cttattcgtc tttcttcttc
    39541 catctgggta aatggcgaca aaaggttttt ctcgccaatt ttatgaccaa gttctgaatt
    39601 tcattggtgg ttcatacagg ccatctgacc attcgatcaa atcatcgatg gtttgtgcag
    39661 ctaataatga acgtttcgct tcactacgtt tgatgcctag atcttctggt aatttcacga
    39721 tgccggctgc acgcaagcta tctagtgatt ctttcgtgct agtcattcca agaggtgtaa
    39781 caccgccaat tgctgcaatt gctgcttgtc gttcttcttt tgtttttgct ttgtacaaat
    39841 aagcaattcc ttcttctgtc acgatatgtg tagtatcttc gctataaatc atcactggtg
    39901 cattggctaa gcctgcttgt ttttgaacag ctagtgcatc taattcttcg acaaagactg
    39961 gttttttatt tgaaccatat gtttcaacca tttgaacaac taatttttgg cctttcccta
    40021 atggatcatc attgtcgcga agtgattgcc atgctggtgt agaatgacga cgaccgccag
    40081 gattatgtcc catgttaggc gcaccgccaa aaccagataa acgtcctttt gttacggtag
    40141 aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat aaacatatcg atggcatatt
    40201 gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt gccgtcttta ccgacaaaaa
    40261 agacatctgg gcgagcagca atatattttt ccataccaac ttcgccacca aaactatgga
    40321 tgctttcaac ccaacctgat tcgatggctg gaatcatcgt tggatgtggg ttcaataccc
    40381 agttcttcgc gattttccct tttaacccta gtgattcacc ataagttggc agtaacaatt
    40441 cgatagctgc tgtattgaat ccaataccgt gattcaaaga ttgtacgccg tgttctgcgt
    40501 agattccttt gattgccatc attgccatta gaatttgcaa ttctgtgatg ttttgaggat
    40561 ctcgtgtgaa taaagcttct aattgataag gtttatctgc tttcacgaca acgtctaccc
    40621 agtcaccagg gatatctaca cgtggtaaag aatcgacaac ttcgttgact tgaacgatca
    40681 cgataccatc tttgaaagct gcagcttcga tgattgttgg tgtttcttcc gtattgaaac
    40741 ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc tactaaaaca acatttggaa
    40801 tcaaatcgat gaataagcgt ccataaagtt ccaaataagt atggatatca cctaatttca
    40861 ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg tgcaccagca taagagaaat
    40921 cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg ttctggtcgt gagatactag
    40981 acatgatcaa atgcagatca ttcacttttt ctgcatctac tttttcaaga ctttctgaaa
    41041 gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac tttgtcgccg ggagcaatca
    41101 gtttttccat tacttctacg attttttcag tttctacata cttgccattg agcaagttgc
    41161 tgattttttc caatcgctgc gctttttttt cgcgtttaga agcccagttg cggtttggtt
    41221 tttccataat tagtcatctc catttttgat atagaaacgt ggcgtcatca ataccatgtt
    41281 ggtctagatt gatcgtacgg atccctttat ctacaccaaa ttttgtatga atcagctttg
    41341 tcaaagtatt gccgggggga aattcaatcg tcagatccat tcctaattct tttgccaagt
    41401 ccatcatttg gttccactgc acagggaaag ctaggttttc caacaaatct ttacgaatct
    41461 cttctgcgtc ctgagtggct cttccatccg tattttttat atacaaacat ctagcatcag
    41521 atagatggac agactgcata tacggcttga atttcttgac cgtatcattc atcaagagac
    41581 agtgagatgg cacagggact ttcagcagct tcgctgtttg cgcttggtgc cgtttggctt
    41641 ctgccaacgt aacggataaa ccttcgatac tcccagataa cgtatgttgc atcggacaat
    41701 tttcatttga cagataaact ggatggtcag gatcaaatgt ttttgctaca atttcctcag
    41761 cttcgcttct cgtcacacct actactacgc ccattgcata accgcttgga taagcttctt
    41821 tcattaagac tgcgcggtga tagactagtt ccgctgcatt ctcaaatctc aaagatcgac
    41881 aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc tgcaatatca ggctgaatac
    41941 cgtttttttc catttcatct aaataaaata ctgcttttgt cagtaatgct agctggataa
    42001 aaaccgtatc ttgatagttt tcttctttat caactaaccg ataccccgtc acattttcta
    42061 aacgttccat gtacatttca gggatatccg ctaacatccc taacttctga ctgccttgtc
    42121 caggaaataa gaaacctgta ctcatgagcc tcgcctcctc agcacttact ttttccactg
    42181 ctatcttagt caatttctgc tataataaaa aatgccaaaa aaagaaaaaa ccataacaaa
    42241 aaggtatgga tgggatggat aattatgaaa ttgcttcatc tgcagtattt taaaaaagta
    42301 ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat ttatttctca acctgccttg
    42361 agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc ctttgtttta ccatagcggc
    42421 agaaacattg agacgaccgt ttatgcagaa gaattttatc catatgcagt caagacatta
    42481 gacacattag aagaaggaat caaattattg tcttcggtca atgagaagat cgttacttcc
    42541 gtcatattgt atttagaggt agcctctgtt tctattccga acttggtccg aatttttatg
    42601 gaaaagcatc ctgatatcca attaacgatc atgcaacatc atttaccaga tgatattacc
    42661 gatcctgtac tgtacatcac ttcggagatg aagcctggca tgaccaatat tcctatcatg
    42721 aaagaacctg tttatgtcgc cattcctaag aaacatcctc ttgctaaaaa agaacatctg
    42781 caattagaag atatccaacg cacccccatc ttgatgctgt caaagagcaa cgccttccgt
    42841 catacactgg atgcagcact tgaagcgaaa agtatcgaat tgacgatcag ttcgacgaca
    42901 gatgatcctg cgacattacg ttctattgtc aagcaaggat tagctgttag ctttttccca
    42961 aaagtttctt ggtcttacga taaaaatgat cctttcgtct tgcgggaaat caaagatttg
    43021 cccttgacac gaacaattta tttatcttct agcttcacag aagatcatcc tttgatcaaa
    43081 atgattgcaa aaacgatgcg ggaattttat ttaggaaaat aaaaaaaaaa gagctgggac
    43141 aaaattcttg tcgcagctct tttttcgaac aaacggtgtt ccagaagtaa tttcttagaa
    43201 aataagccga aatttcccaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    43261 tgaagctaaa catttctgtc acaacctctt ctctattgtc ctagacattc attgcgttga
    43321 tttaaaactt ccatcaatca aatctgctag ttcatctagc ttttctattt tacgtagatc
    43381 aatagagatc caatgttctt tattcatgtg ataggctgga aaatacccct cttttcctcg
    43441 caaaattccg ccaagttcag gatcgacctt caaatcaaga atattgatga tcctatggtc
    43501 acttgcatca tcatagattt tttcttctgg aacagacatc aggaccccaa accatttttt
    43561 gcttttttga tgtctgaata cgcaataatc aggaaatttt ttaaaaatat attctggaga
    43621 caaattgtat gtttcattga tgtgatcgat tagttctttt ttcgttagca tctgcttcca
    43681 tctctttctt attgattcgc tttattcaaa gataccacgt aagaatagaa aaatgaatac
    43741 aagtagttgc taatcaaata cttcgatatt tttctttttt atcaactctt aaatgttcaa
    43801 tccgcctgtg ttggtcttgc taccatctac tattaaaatg tttattattg tttgcccacc
    43861 tattttttga ttttagtggt atcatttttc aagttcatga tgaaattctt atcatcagta
    43921 aatgctccag aaaaataaac aatgattgaa aaatcagttg aaacttgacc accgtttatt
    43981 ttctccgtat tcttagaata gaagggagtc tgattgatgt attcactaat gaaaaaaatc
    44041 attaccgaaa aagatattcg acgtcatgtt tctttagtag aacaattgct gaatcataca
    44101 aagataaccg taaatgaatt agctgaaatc atagggacaa ccgaacgaac gattttttct
    44161 gaccttcaat ctattcgctc tcagctgccc gaaggctggg acatcatcag cgatcaagca
    44221 ggaatcagtt tgcaaaacca gcaaaattta ttaacaaatg acctttggga gatttttttc
    44281 aaacaatcag tcagtgtcga actgctgaaa aatctgcttt ttacaaaaaa agttgctgtc
    44341 cctgattttc tagccgacca tgggctttct tatgggacat tgaaaagaca tgtgactaaa
    44401 atcaatcagc gtttagcaag ttacgacttg cagatcgatt tgacgaaata tacagcttgt
    44461 attcttggaa aagagcgtgc gattcgtact ttttatcatc gattgctgat tcctttcact
    44521 cataacaact attttttcga ggattattcg atccatgaat cccattattt tcaattttta
    44581 agaaacctca gtcaaactga actagcggtt gaaacagaag agattttcgg aacatgttgg
    44641 ttctttatca atacgatccg tataaaagcg aattgccgat tagattcttc tatccatacc
    44701 aacagcacac tctcctccct gtatgactct gcattgaaaa aattgtattt aacggaaggt
    44761 atttatctta aagacactga gctttctttt gcttctttct gctttctgga aagttggaat
    44821 tacaacaata actatggaca agaaaccgct cgctgcctgc atcattctcc atttttagag
    44881 gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt ctcttgatca actaaaaaaa
    44941 acatctctga ctgataactt ggctctcctc atattaaaat atcatgaatc gccgatattg
    45001 attgaagcat tggaccgtca ataccatcgt tttttagaaa catatgatca gcaatatgca
    45061 cacttgtatc aacagaaaga gaatttactg aacgatctaa aaaaagaaat agaaatcaat
    45121 gaaccaaatt actttctcca actcttgtct ctactgatcc aacaaacgat tttttctatc
    45181 aaaccgaatt tgttcaatgt ctattttatt ttccaaggag aaccttcatg gaaggcgttc
    45241 ttgcagcagg aattaaaaga ttacttagga aaacgtgtct gcttcttacc tatcgaacta
    45301 accgatcttt ccactatttc ttttgaaaaa acagatatct tgatttccaa tttccctcta
    45361 gattctctag atatccctat tgtatacatt tcttctattc caacgaaaaa tgagctcaat
    45421 cgtttgactg aactcacttt caaaactttt ttatagtaac ggctagcgac tgtattctct
    45481 agctgttttt tcttttttgc gaaacagttt gttcatgatc cctttttcta tttctacaac
    45541 taagaataga agaatcacat ttgctccaat caataactgt tgtgtcatcg ttaaacttac
    45601 tgtaccgatc agctgctggc cgactggtag aaaaataacc atcccttgca ataaagcaag
    45661 gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga ccttggttca atgacggctt
    45721 ggacaactct ctgcaattga tcatgtaaac agcttgagct aagacgatat tttgcagaag
    45781 aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc aataaatagg caggaaccat
    45841 gatcagtagg gagacataga tgatccgaaa caaatgataa ccagatagga ttccttgctt
    45901 tggatctctt ggtgggcgct tcatgatttc tggatctggc gactcgaacc ctagtgcata
    45961 agataaagtg attgtcgtta ccatattgac ccataaaatc tgtactgggg acaaaggtag
    46021 cgggcgattc agcataagtg cccaaatcaa gatcaatcct tgagctaatg cagttggcaa
    46081 gaagaaattg atggtttttt gcaaattatc caaaatccgc cggccctctt ttacggcttt
    46141 tgcaatcgta tggaaattat catctcctaa aaccatatca gctgcctgtt tgcttacttc
    46201 actacctttg attcccatag caattcctac atcggatctt ttcaaagcag gtgcatcatt
    46261 tacgccgtct ccggtcattc cgacgatttc tccatttttc tgcaaagctt taacaattcg
    46321 cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact attggcactt gctgaatcaa
    46381 ctcttcatca gacataagat ccaaatcgat cccttctaaa actttggcag tatgttttaa
    46441 gccaatttgt tccgcaatcg cttttgctgt gtctttgtga tcccctgtaa tcattttcac
    46501 tgagatccct gcttgttggc attcctttac agctttgact gcactttctt ttggcggatc
    46561 gatcaatccg gcgatccctg caaaagttaa ttgggttaaa gtctcatgcg tgagttcctg
    46621 tttcgaatct acttttttat aagcaaatcc taaaacacgc tgaccttttc tcgcaaattc
    46681 tgcagctgtc tgactccatg cttcttgttc tgcagaagaa agagttgatt tagcaaataa
    46741 tacttctggt gcacctttga cgtaaatcac cgctcctttt tcctccgcat gcatcgttgc
    46801 catatatttg tattcagaac tgaacggaat ctttgcttct atttttttca aaggtagatt
    46861 ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga ttgccactta aattttctgt
    46921 tttctgttct tcttttgttt ttaattcctg acagtttttc ataatggaca acacgcaggc
    46981 ttcttctttt gcagctacat ccacaactgt catttcattt tttgtcaatg ttcctgtctt
    47041 atctgaacag atcactgtca tagaacctaa tgtttctacc gaaggaagtc ctttgatgat
    47101 cgccttttct tttgccattt catgtactcc aacggataga atcatggtca aaacagctgg
    47161 caatccttcg ggtaccattg acaccaccaa agcaatcgta gccgaaagaa ggatatgcca
    47221 atccatacca tatcgaaatg tcgtaaaaat aactaagaat aggctcaaac aaagaatgcc
    47281 ttgaaaaatt tgtttgttta atcggttgat ttttttaatc agaggcgtcg tttgttgttc
    47341 gaccgattgc aatgcttgat tgattttacc gatttctgtc gtgtttcctg tttctacaac
    47401 gacaccgact gcagaaccag attgtacaag cgttcctgaa aaagccatat ttttctggtc
    47461 gccgatcgat tcttcttctg tcaaaacggc atgacttttt tcagatgctt ctgattctcc
    47521 cgtcaaaatc gattcttcca ctaataaatc atgtgcttcg attaatcgga tatctgctgg
    47581 aacgacatct ccggcacgta agacaatgac atctcctttt accagttctt tcgcagggat
    47641 tttttcttgc atcccgtttg atagtactat cgcttcttgt cccatcagct gcttcaaacc
    47701 attcaatgat tcttccgctt ttctttcttg ccagtaacta acgagtccat tgacgatcac
    47761 tacgaataaa ataatacttc cttcaatata ttcactagaa gcaaatttca aaaaagaggc
    47821 aatgatcaac acgatcatca gcagatcagt aaaatgcttt gctatttttt tccatcgttt
    47881 tgtctgctgc cttgctttta tttcattata tccttgatca gtcaggcgtt tctgtcgttc
    47941 ttcttgcgac aagccttctg ttgatgtttg atagtttttc aatacatctt ctaccgtcaa
    48001 tctgtaccag ttcatcctat cccctcctgt ttaattgata ctgttattat acgcagggaa
    48061 gttttttcag gcgtatcgat tatttcaggg gggtctgaaa taattcgttt tgcaaaatgt
    48121 atctgggcct aactgatatt tctattgctt attcttttat atacaaaatt taagaataca
    48181 aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc tttcgaataa caagctgaaa
    48241 gcaattgaag taaaaaacac caacaaagca agatctttat atctttgtgt agtgttccgt
    48301 ttcttttctt taataaattg aacatgtgca cacaagatcg aagaccacac aaatagaaaa
    48361 caactcgttg tcatgcctgc caaaagctga aaaaacaatc gactatcagc tacaaaaaaa
    48421 tgtagacaaa gaccggcaaa aaataacatt cctgtaatta aaataccgtg taaaggtacg
    48481 cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt tttcttttgc tgtttgatat
    48541 aaaatcctgc tagtactgta gactgcacta tttgctgagg acaaagaagc aaccaaaatc
    48601 attagattga caattgctgc tccgccttgg caatggatca cttgcagcat agtcactaaa
    48661 ggactttctt ttaaatctaa acaatgccat ggaacaacaa ataaaatcga caaaatcgtt
    48721 cctatataga aaaagagaat acgccaaggc agacagttga ttgctttttt caaattcttt
    48781 tgcggatcac ttgtctcgcc aacggttaag ccgatcattt caattccagc aaaactgaat
    48841 aaaaccatag gaaatgagac taccattcca gcaaagccaa acggtagtaa ttcatcgatc
    48901 tgtccaaaat tttctttgat tccgatagac tgggtatgaa aatctgagac actcaatcct
    48961 atacccaata agataaaaag gaaaatcccc aagatcttga tgattgagaa aaaagattcc
    49021 aattcaccaa accacttgac tgcaacaagg ttgactgcca ttaagatcat tagtgcgcta
    49081 cctccgctta tcaaagctgg tacatgtaca aaccagaaat ggatatacaa tcctattgct
    49141 gttaattccg caagtccagc tccaagccag caaagccaat aagtccaatt gacaaaaaat
    49201 gcccactttt tgcctaaata atagtcaacg aaatcactga tagagttaaa tgtactattt
    49261 gctaaaaata aatcacccat ggcccgcatc aaaccataaa tagccaaacc gcatatcaaa
    49321 taaacgagaa taacagctgg ccctgctgat gcaattgcct ttgctgaacc taaaaataat
    49381 cctgttccaa ttgcgccacc taatgcgatt aactggatat ttcgattagt tagagtccgt
    49441 ttcattgccg gtttttccat caagttcacc tttttctttt tcaagcagca tggcgacttc
    49501 ctgttcccaa gaaggtttct ttattttttc aatacagagt ggtactacaa acaaaatgac
    49561 actgatggct gtgactgcaa ttagattgat ccatgttcca gctttcatcg tcagtccgac
    49621 agaaaatccg attgccgcaa ataaaacaat aacgacagtc caaactccaa gattgctctt
    49681 tttctctttt tttccaattc taaatggtcg attcaacttt gcatgtgtat aacgcaatct
    49741 caaaactgcg atccccatga tgacatagac gatacagtag ataacagtag ttgaatttac
    49801 gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa taaaaaagtg caaagattga
    49861 gataatgaca gactgagtca aaataacgga tttggatacg tcaaattgat tcgtcttcca
    49921 aaaattgaat tttggtggat acgcaccttt tctagcactt tctgtcactg tctttgctgg
    49981 tcctgaggcc caagcagata attggactgc tacaccaatg aagacaagta aactaaacag
    50041 attcacaata agatgtggca aaccaagaat ttggcaataa atcgaaatag attgcgcgat
    50101 attattcaat tccatttgtc cttttggtac gacatttgct acaactaaag cattcaaaat
    50161 attgacgaaa aatataaaaa ttaacgcggc aaatactccg cgaatatacg ttttaactgg
    50221 attttccaat cgagtgatat acacagagga catttcgata ccagtaaaaa taaacatgat
    50281 cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct ggtattaatt tgtcccatga
    50341 aaaactgcct aatgttccgg ttggtacgat cccaactttg atccacgcag caaaccctaa
    50401 taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa acaccgactt ttccgccgat
    50461 ttttgccata tcaaatttca aattcaaaag agtgatgatc caataaacga ctaaaatgac
    50521 aactaatgta aatttgctgt tcaaaccaag cggcacatta tcgataatat taccaaacaa
    50581 cggagcgagt gctgaagcga ccatcaccat cccaggaaac atttggaccc ataacagcca
    50641 tgagacaacg aacccccatt tttttcctag agcattcgat acccaaagtt ctggaccacc
    50701 tttttgggga aacattccag caaattcacc agagatcaat gagattggaa aagcgtacaa
    50761 taatactgca accagcatgt aaaaaatcat cgtccagctc gttgctgcta catctggaat
    50821 atttcgtaca cttgctacaa gggcgcaggt cattccgata aaggtcatgg tcgtgatttg
    50881 ctttgatgaa ccagtatctt tcatctttat tccttctttc aatagcgaca tatgtttttg
    50941 ttttctttta tttttgttca agaacagaaa tgacaaggtt tcatcgattc agtacaaaac
    51001 ccagtatcac cgaaacatcc attgaacgat cttagtgaac tgggtttgat acatgaaacg
    51061 aacgatccaa cttatagaac gttttataaa aaagaatatc ttggcgagga atatatgaaa
    51121 aacaggggga aatacaattg ttttgatgaa gcaaataatt gtctgttctc catatgtctc
    51181 ttgttattct ttcacacaaa aatcagttct attggatgat ttcgtttagt ttcttttcca
    51241 ttgctttggc gatttttttt gtataaaaat tgaaaattcg atcatcattc aaataaggag
    51301 tcatgatagc cgcacgtagc agagtgattt tctgttcttt tttccattct tctttcgtta
    51361 gacccattct ttcaacaaaa cgaatcggag aatcaccgta atcttcttgt gtaaatgtag
    51421 tatgagaagt aataaaccgt tcaccataaa catcgccatc catataagaa gaccgatcga
    51481 acattttttc atttaattca ttgatagcgt tcaaatctgt acagccttgt tctttgaata
    51541 cccaattgac catattaaaa tcaggatgat tcaatggata gacttcgatt gtttttcctt
    51601 taacagtaaa agtcagatga tcgagaaatt ctctgaatct ttgagctgcc tcgattgaag
    51661 ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa tacacgatgt gctgtccaga
    51721 ctgcggcggc ggttgctcca gcttttgatc cttccaaaat ataagctcct aacatatctg
    51781 gagcctttac tgatttttca aacacatatg gcgcgaaata agaaatgata tttctcatat
    51841 ttttatgttt gattacgatt cctccagcag cataagggac atagcccatc ttatgaggat
    51901 ctatcgtgac agaatccgct tctgaaatag ctctgaaccc ttcatatacc tctttgtcaa
    51961 tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa ttcagctaag gaagcatacg
    52021 gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc atagccgcca tatgcggcat
    52081 caacatgtaa atagaaatag atcccttcat cttttaacct ttctcttaat tggacgattt
    52141 tatctacact gtctacttga ccttcttctg tcgttcctac gacagcaacc acacctagaa
    52201 ttgggatttt ttgtccagct aattcacgaa ttgttttttc caaagcatta atatccattc
    52261 gataatcctc ttgaactggg atagcaacca tctgatctaa gccgactccg caaatatcta
    52321 acgccttcat ccaagaatag tgttttgttt gagggaccag ccatttccct aatctttgga
    52381 tattttttcc gctacgggaa gaagctgctt tgacctcatc catttcttcg tccgtaaagc
    52441 tttcggtcat ttccaaaatt tcttcaacag ataaattcaa aagttcccac tcagacattt
    52501 ttttcacttt ttcaggcagc acttccttga cagctaaagg aatcgatttg atgcaacggg
    52561 cgtaccataa tccttcaaga ttagcaatag aaccatctgc tgcaatgtgt ccccatccgt
    52621 ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc tgcttccatt tgcgaagttg
    52681 ccatagaaga ttccaatgca acattatttg ggttccacag catcgcatag ttgtaagcta
    52741 ataaagcagg catcaatgtt tcagcattca tttgtcccca gtaacggcct gctgaatgcc
    52801 atgggatcga tcctgcgcgt aatcgctgac tgacttcatc caagacctct atcatatggt
    52861 cgcgggtggc tagataatct ggagtgagct tatctccttc gctaattgca ggcatatctg
    52921 aaggaatata attttctctc catccaagat gttcatctac catcttgttc aacaacattt
    52981 tataaactgg tccattttca gctttgtcgc ctataaagac ggccttgata tccatatctt
    53041 tcatttttct tccctccata ttttgttggt caatgtcttg cagatacatg tgtttatctg
    53101 taacacctct tcactatagc gccaaaaatg agaaattttt ttttataaca tcaaatccac
    53161 gtagattttt acaaacaaaa ataaataaag tcggattttt ctgataattc aacgcaaaaa
    53221 agcgaacaat ttttcaataa atcagtcttc taaaatagat aacctaaaaa aagcgaacaa
    53281 acaaataaaa tgactgtcct tcattcacta aacatatttt tattttttta tattgttaaa
    53341 aattcacaat ttatttttta atagaattat ctgttttcca aattttataa cataaaaaac
    53401 cttttttaac ggcatttata tgctttttca gaaattaact gatgatttca aaaagaaaac
    53461 atgtcataat atttcccata ttgtgcaaaa aaatacaagg cggaggctct actatgattg
    53521 aagaatatat tgagaaaaat attttaagac agctgttttt atgtgggcaa ttctatgtaa
    53581 ataaagaagt caatctagaa aagctctcca atctcctgca tgtttgcaaa accactttac
    53641 tcaatgatat caataacata aaaaaagaat ttgaagaaca gatcgcttat acacatcggg
    53701 aaaaggaccg ttacacttta tatttttcag aacatatacc tcgatgcaag atcatgcagc
    53761 aattgtctca aaattcgttg tttttaaaaa cttgtctgct ttatttggaa gaagatgaac
    53821 ctgattatct tcaattaact gaatgtgaat tcatctctgt cagcaaagct tattcattaa
    53881 aaaaacaagt attagcttac tttaatgact gcgggatcga aatcgaccgc tactcccctc
    53941 gattcactga aatggaaaga cgtctgctgc ttcttaatgt gtcgtatcgt ttaggcggtt
    54001 ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc cgatcgtttc attgaatccg
    54061 taacggaaaa tagtggcaga ttttatgata aagaaaataa agaaatctta tcgattggtt
    54121 ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac aatagattca aaatttatcg
    54181 aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc agcatgggaa aacactgatt
    54241 tccaaaccta ttataaaaaa gaagaattcg cctttatttt gacactattc aatctttgta
    54301 attatggttt ccattcttat cagttgattg cggaagactt tcaacagcta caccaagtgt
    54361 ttattgacaa tactcccgaa ataaaagaac tagttgctac ctttgaatct cacttcaatc
    54421 aagaattatt cggaaaccaa ccgttcgaac gtgcgctgat tcatctgatg cgttcggctt
    54481 gggataacta tcaattattc atgccagaaa aattttatct tttaaatgag gaacagacaa
    54541 atttatataa agaagtccag acaatttttt ccagctggtc cagccagtta ccttatgatc
    54601 tacggttgaa tcctaattgc atgcgggctt ttgtgatcga attgagcgga attctccgtc
    54661 tgacaaaaga acatctgact atctatatcg tcacaaattc ggatgttcat tatctgattt
    54721 atcgggaagc cttggaagct gtaactactt tcgattttca agttgcacca acaatttatt
    54781 caagtatatc tgatataaag aagtatgcgc agcagtcctc aaaccgggta ttatgtgaac
    54841 gtacattata tacaccagat gcggtccaat atgaaaatat tattcctatt tcgatcaata
    54901 cgatcgatcg agctatcatt tcagctgtgc aaaacaaata aggggctgtg acaaaaatca
    54961 tgtcacagcc tcttatttgt tgaagatgcc catttctgta acaatctctt gatacacgtc
    55021 aggctcacaa cctcacttat tttggacatc taggggtccg atctctttat ttaaaaaata
    55081 agaaatgatg gttcggataa ctaccaacgc cgctagttgg aaaatatcct gcaaggtagg
    55141 tttgataatc gattcgatga tcccagctga aacaagaatt tccaaactta gaagtatata
    55201 gcctcccaac atctttttaa tagcattgtt ctcgcttact cggtcgcttt gcacttttgt
    55261 atggacaaat aaatatttca aaaaatcctt gattgataga caaacacccc aaaccaataa
    55321 acagatagca aagatatcaa gtcccagtac aagtttttcg aaatatggaa gaagaaactg
    55381 catcaactct gtcgtcatcc tacctctcct cttctttgtt tttctttgtc ttctatttcc
    55441 tgattcaaga aataagaaat gactgttcga atgataataa taagtgcaag cttcaaaata
    55501 tctgtccaag tcggcttgat aactgactcg atgatatcag caacgactag tacttccaag
    55561 cttaacaaaa tataacttgc tagcagtttt cttgtatctg cattacgagt attacgttct
    55621 gcatagtttt tcttgacatg agaccaatca aaaagtttga atagaacaag aacgatcccg
    55681 acaattaaga taccgataga acaaatattt aaaatagcaa tgatccattc tagaaattca
    55741 attaaatttg agcgaaacat atacgctacc tactttcttt taatttgttc ctattctttt
    55801 attgtaaaag aaagttacct tgttcgtcta tttaaaagtt gttctaatga taacggttcg
    55861 cttaaatttt tctttttcac tctcccattt ttctccctcc atccataaat cgtggaagta
    55921 tccaaaacaa cgtagcaagt taaaataagg cttagtccgt catcagcttt tcaaagaagc
    55981 actgtctcgg tgcttttttt gtacccaaat ttctaaaaat agaaccatac acctcctcaa
    56041 atcttttctt cttacctacc ctatttttat ttacaggaaa atcagacact tcttcttttt
    56101 tgtcaaaatt tcctttttct tctcgtccta tcatttggca ttctaataaa aacgaactat
    56161 ccttggatat agaaagagaa aacaaggagg cgtttttcat gagtaaaaaa ttaatcaaag
    56221 tcggcatcgg tttaggtttg cttgcccttg gcgctgcata tctaggaaaa aagacaggac
    56281 tattcgaaga tgacagtcat ttatatgatg aattcgaatc aatctagcat cctatttagt
    56341 tgaatcacta cggacatact tggaagacat tggaggaatc acaatgagaa aaagtaaagc
    56401 aataatgatt ggtgccggta ttgccaatat ggcagcggct gtttatttga ttcaagaagg
    56461 caaatggaaa ggggatcaga tcacctttta ttcattggat gatcacggtt caaatgatgg
    56521 tgcaccgacc gacactgtag tggatgaata ttggaataaa aaccatccat tagaaaacca
    56581 aaaaggctat attgctcgag gcggtcgcat gcttaattac cgtacctatg tagatttgat
    56641 ggatctgtta agtcgaatac cttctgttac tgagccaggg atgactgctg aagaagatac
    56701 gagagatttt gatgcaaaac accgaacctt tgacaaagca cgtttgatgg aaggtggcaa
    56761 aggaatcatt gatggcggaa aactgggatt caataataaa gaccgtattc ttctgactaa
    56821 actgattgct atgcctgatt cagaagaaga aaaactagac aatatcacta tcgaagaata
    56881 tttcaaagat gatccacact tttttgaaac aaacttctgg tttatgtggg aaacaacatt
    56941 tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc tatatgcatc aaatgattta
    57001 tgaattcact caaatcgaac atctagtggg cgtcaatcga acacgttaca atcaatacga
    57061 aagtattatg cttcctttga tcgagtactt gaaagaacaa ggatgcaaaa tcattttgaa
    57121 ccgccgtgtc actgactggg aattcaaaga aacaccgatg caagatgaaa tcactgtgac
    57181 tggtttgaaa attaccaata cactgactca agaagaagaa tacgttgtgg tcgatgaaga
    57241 tacagcagtt attttcacta atgggtccat tacagattca gctactttag gagattacga
    57301 aacaccagct gtagaaaata tggattatgg tgcagcttct agtctctgga aaaaagccag
    57361 tgaacgtttc tacaacttgg gaaatcctga caaattcttt gctgatcgag atgcaagcga
    57421 atgggtcagc tttaccctga cgacgaaaaa ccatttgttg ttgaatgaaa tcacccgtat
    57481 cacgactcaa gaaccaggaa atgccttgaa ttcatttatt tcaaccacac cgatcacgcc
    57541 gttaggccaa aaagatgtca atatgtctat tgtcgtgcat catcaaccgc attttacgtc
    57601 tcagaaacca aatgaaacag ttatttgggg ttatttcctt tatccaagac gacgtggcga
    57661 atttgtggat aaacagtata ttaaaatgaa tggaaaagaa atgctagaag aactgattgg
    57721 acaactttca aaagtagatc ctggaccagt gaatattcgt gaaaaagaaa cagaaatttt
    57781 cgacagcgta ataaataata tccctgtcta tatgccttat gcttccgcgt tgttcaataa
    57841 tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat tcgaccaatc tggcctttac
    57901 aggagaattt gtcgagcagc cttatcagat gatctttact gaacaaagtg ctgtacgttc
    57961 tggagaaatc gctgctttcc attttgcggg tattcctatg tcccgtcttg tcaaaacacc
    58021 gcgttacgac aaagatattc ctactctagc tcgtgccgca aaaaaaatgt ttgaataatc
    58081 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acaaccaggc taaaacagct
    58141 tcttatgttt ttgatttggt tggacttatt accgcagatg caagcttttg aacaccgttt
    58201 accaagaggt tatgaaaaag ctgccctgca tcaagtaata agagcagcca agctaaaaca
    58261 gcttcttatg tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg
    58321 tttaccaaga ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa
    58381 atggttcctc acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc
    58441 ataacctctt tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac
    58501 tctcttgcca atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact
    58561 aaaaaatcgg aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt
    58621 aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt
    58681 agtactaata atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc
    58741 acttggcaag gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa
    58801 ccaacagatg caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt
    58861 taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga
    58921 atcgttgaag aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc
    58981 ccttttaata ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt
    59041 gatgaatcaa atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt
    59101 aatcattggg gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt
    59161 gttgtaccag ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc
    59221 acagcttatt ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta
    59281 ggttctggtc cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc
    59341 gtaatcgcag tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt
    59401 gaaacttatt tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa
    59461 ggtggcgcag atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa
    59521 aaagcgaaaa atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg
    59581 caagctgtca aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct
    59641 tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca
    59701 attatccatc taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca
    59761 caaatcatta ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac
    59821 aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag
    59881 gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa
    59941 gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg
    60001 atctcactgg ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc
    60061 ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg
    60121 acgattcgtc ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca
    60181 atgaagagaa caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt
    60241 ttcaccctat cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca
    60301 ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa
    60361 gtttttcgta cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta
    60421 tcccctgctt acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag
    60481 ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat
    60541 ctaagaccac ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt
    60601 cctcatacac aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta
    60661 ctaattctgg gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg
    60721 actaaaataa aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca
    60781 atagtgatgt aaaaaaaaaa acggaaaaca atcacttgtt ctccgttttt tgattaatca
    60841 agcatctaaa gcttcttttc ttctttcttg gatcgtagct accttgacga acggtagata
    60901 gatcaaaata gaaagaacca aattgacagc ggctagtaca ccacctgcaa tactttttgt
    60961 tgccaataca ccgccaataa ttggtggagt tacccatgga ggcataacgg tagctgctgg
    61021 cactaaacct gtagaagtcg caaagtaagc aacactaact aagaccattg gtgtcaaaat
    61081 aaatggaatg aacatgatcg ggttcaacac gataggtaac ccaaacatcg ttggttcatt
    61141 aatattgaag acacctggcg caatcgacaa gtttgttaca accatatatg gtttgttttt
    61201 acgtcctact aaataaatag ctaacagtaa acctaaagtt gcacctgttc ctcctaagtt
    61261 gacaaagctg tcaaagaaag gtttgttcac gataaacggt gcgctatgtc cagctgcgat
    61321 tgcattaacg tttgcttcaa tggctggcgc attgattgtt tgcatcaaag gatcgaccat
    61381 attcgctccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcagtaaggc
    61441 agatggataa gagtttgcaa gtcccataaa tggttcttgg accactgtat agaaagaacc
    61501 tacgatatca tcaacaccga atgcagcaaa aattgcagca actaaagcaa acgcagagat
    61561 agtgatcatt gctggtagta atgaagcaaa tgcttttgca actgccggtg gcacaccatc
    61621 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
    61681 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
    61741 agcgaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
    61801 agctaaatga taagcaagca agaaagcaat caagacagaa agaatggcaa atgtaccgtt
    61861 ccaagcagca gccccgaatc ctttccatga ttcaccacca aaaattgagt tcatcaattc
    61921 ttggaacccg ggaatcggta agttattgat cattactgct aatgctccta agatcattaa
    61981 cggcatactc accataaatg agtcccgaat cgcaactaaa tgccgttgtg caccaatttt
    62041 tgatgctaca ggtataaaat gtttctccat aaatgcaaca aatttatcca taaatcttcc
    62101 ctcttttctt tactgtagat tacgcgttca atcgacgata cagattaatg atctcttttg
    62161 ctaaatctgt aaaagctata gatgtcatca gatgatcttg actgtggacc gtcaaaagcg
    62221 tgacttcaac gtgatttcct tgtgcttctt gtgtcagcat ctctgtttga gagtgatgtg
    62281 cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg aaatcacctt
    62341 ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca aacatgatca
    62401 gacccatgat ggcttctaga tttttttgat cttccattta ttgtatcctc cgataatttt
    62461 tattatttgt ttattagttt ttctgcttgg tcgagtactt tttcaccatt catcatgcca
    62521 tagtcagcca tattgattac atctaaagga atgcctttag gtgctagctt ttcagcaaat
    62581 tgattttgca taaagcgtac ttgcggtcct aaaagtaaaa cgtctacatt tttagattct
    62641 agttgattat ccgcatctga agcagataca gcaaagatgt ctgcttccat tccttttgct
    62701 tctgctgcct tttgcatttt tgtgactagg agacttgtac tcattccagc tgaacataca
    62761 agcatgattg ttttcttttc cattttaaac acgttccttt ctatacttac acttgtatat
    62821 aagcaagtag cgtgccaact ttaaaagcgc tttaaaaagt tgatatcatc acatttaagg
    62881 tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca caaattaatt
    62941 tttattacct tttttctttt aacgtgcaca aaaaaagttg tgacacaaaa cttgtcacaa
    63001 cttttttcaa ataaacggta ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
    63061 aaaaacataa gatgttgttt ttactttgct attcttatta cttgatgcag gacagctttt
    63121 tcacaacctc tcaataaacg gtgttcaatc agctgaccct gggtattagc tcaaaacgct
    63181 ggattcacaa cctcttttat ttcaaataac cagtaaatac aatgaacatc tacaaaaaac
    63241 ttctatttat ttatagattt cgtcaggcaa atccaattct ctcttatcaa tcaaagtctg
    63301 ataccacgaa gcagattttt tcaatgttct gcttcgttca tcgttcaatt cgattcgaac
    63361 taatccatat cgattcttaa atgcattcat tggtgacaca cagtcagtga atgcccataa
    63421 catatagcct tcgcaatttg cgccttcttc tgttgctttg atcaagtaat gcaaatgctg
    63481 tccgataaat tcgatacggt aatcatcttg cacgattcct ttctcatcca tatactgttc
    63541 ttcatattct cttcccatac cgttttctgt tatcagccaa gggatattat gataattttc
    63601 ttttagatac atggctatat catacatgat tttcggatag atttcccatc ccctggaatt
    63661 attcattttc ttcccaggta aatcaaagac ttcataatat cgttcaggat ggaaaggtgt
    63721 ctcttcattc cattgataac gaggggcgcg aacgcgttta ggataatatt gattgattcc
    63781 aaggaaatct actgtatgat gctgaatcgt ttctaaatcc tctgttgcag gatcgaaata
    63841 aatcgattgt ttttgacaca actccaggaa ctcttctgtg tagtatcctt ttaccatcgg
    63901 atcaaaaaat actcgattat aaaacaaatc atacatataa gcagcttttt ggtcttctgg
    63961 agaattggat cgagcataga ccatttcagg atttaaaata cagccaatcc ttccaccata
    64021 atttccgctg cggtaaatct ctactgcttt agccgttgct aaaactttat gataattcca
    64081 catcatccac ttttttgtgt tttgttcatg cggccagcga acagcatcca aatagcaacg
    64141 cgtttgggga acgattggtt cgttgaatgt aaaccagttt tttaccctat caccgtaacg
    64201 atcaaaggca atttgagcat acttagcata aaaatcaact acttttcttg aagaccagcc
    64261 atcatatttt tccaaaagaa aagctgggac ttcataatgt tccaaacaaa tcatcggttc
    64321 tacgtctgct tcgatcaaag agtcgatcat ttcacttata tgctgagcat attcttcatc
    64381 tactatacag ttctcataat cagtaaaaaa acgagcccaa ttgatagaag tccgataatg
    64441 tgtcagatgg atggatttca tcagatccac atcctgctgg tattgttcca taaaattagt
    64501 tgctacggct gggccatacc cattatgcca aacaaaatgt tcatttttat accagctatc
    64561 tataaacgaa tcctgtcctt ctttttttcc tttccaacct tccgtttgcc aagcagaaga
    64621 tgcagcacca agaataaaat tttttggaat agtaattttc atgatttcct cctgtttttt
    64681 agatcccatc attttccaca cgatttgcta cttttacaaa tggcagatag atcaaaatag
    64741 aaacaataat acaaacaagt tgcgtaataa ctgcaccgat atttcctgct gttgccagat
    64801 aagcattgac gatcggcggc attggccaag ggaccattac aactgctttc gccgcaaatc
    64861 ctaaagcggt tgcaaaataa ccaatcgttc cagtgataag aggcgttaag ataaacggaa
    64921 tagctaaaat tggattcagc atgatcggca taccaaaaat aactggttca ttgatgttga
    64981 agatcccagg agcaagggat aacttggtga tttgtttcat atcttcccgt tttccaacta
    65041 acaatactgc aatcagtaaa ccgatcgtga ctccagaccc gcccatactc atatacatat
    65101 cccaaaatgg catagtaata atgttcggta cttcttttcc agcttcaaaa gcttccgtat
    65161 ttacagcaat tgccgctaaa agcaacggtt cccgtatcgg cttcaccatt tgatttccat
    65221 ggataccaat cacccaaaag acttgcgcaa taaacatcaa tagcaaaata cccggtaacc
    65281 cttgtacgac accttctaat gggcgttgta cgacattgta aatgatatca taaaggtaca
    65341 ttcctgtaag ttctttgatg ataaatcccg cagccgcgat acccgataca gtcagaatcg
    65401 tcgggaataa agcagaaaaa ctagcagaaa cattcgttgg cacggtatca ggcattctca
    65461 gctgtagttt cttctgtttc ctcaaccagc aataaagttc aatagaaagg atggagacca
    65521 acattcccaa aaataaccct ttcgtatctg tatattgttt agccaatacg ttttctacta
    65581 taacagtctg attttcattc actaattcat aaacagttgg attaacagat aaataagaca
    65641 tcactgcgat gataccaggg aatacaccat ccactttatt cagcttggct aattcgatcc
    65701 ctatcagaaa aacagcatag atggtcaaga aacttagtgt gacatagctg atggcagatg
    65761 cgataggctt taattcttct aaaaaacgca agaaacttat ctgtgctaag ccattttccg
    65821 agtcaaagac cattgctgaa aacaatgtgc caaaagcacc cgtaataatt accggaatca
    65881 atgcagcaaa tgcgtttttt atcaccatga tataccttaa attattgatt ctcgttgcca
    65941 cgttccctaa aacattggct aatttatcca taaaactcat tccagatccc ctcattcctg
    66001 cttcttctcc ttgacgtcat ctctttttgt ctattagtca gcgcttacat ttcgatgaac
    66061 agaaactatt tactcctctt ttttaccaaa tataaaacaa aaattcatct gatgtattta
    66121 tcataaaata catcagatga actgtcaatt agtttctttt tctcctatta ttttctgtaa
    66181 ttctctccga tttattgcca tatggacagt cattgcatca aacgcagcaa gtgcattccg
    66241 cctttcgatg gcctgaacga tctctgtatg aatatcgatc gtctcgattt tcagatgctg
    66301 gatatcataa tttttattaa acaaagcaat tgattcatta atgattggga taatatgcag
    66361 caaggctacg tttccgctag cttctgcaat catcgtatga aaatcgatgt ctaattgaac
    66421 atgttcttgt gtgccttctc gaaaagaagt ttcaatcttt tgcgttaatt ttttcatttg
    66481 gacgatttga ttcttcgttg catattccgc tgccaaacct gccatcttag gctccaataa
    66541 atatctcagt tcaaataagt ctcgtatcat ccgatcctga tcttcaatca aagaaaaacc
    66601 gagtgggtca ttggcaatcc ctcgcttatc actgacaaat gtccccgctc cttgccgaac
    66661 ttcaaggata ttcctggaag ctagagcccg cacagcttct cgcaccgtgc ttcgccccac
    66721 ctctaaataa tcggacaatt catattcatt cggcagttta tctcctactt gcaactgcat
    66781 ttcctttaaa tatctaagga tcttgtcagt agttacttct actagggttt tattttcttc
    66841 caaaccacat acctcctttt catctgatgt attttttcgt aattattata acacgtttga
    66901 atcatctgat gattttttgt atacaaaatt gattaatccg ttcatagaca tacaaacgaa
    66961 gaagatattt accataaaaa agactaggat aaaattcact cctagccttc gatcattttt
    67021 attttgaacg acaaaaaata tgatgttcaa taacagacga tcttcattcg attcttgatg
    67081 tcatcttttg ttttttttaa tatatggata aaaagtttga ttacatcgtt tctattctag
    67141 aatttggtcg attctcaatt cttcttctgg tgagaaatct aaattatcca tcattcgaac
    67201 actttcttgt aactgactta accgacttgc tccgaccagt acacttgtaa cagatttttg
    67261 tcgcaaattc catgcaaggg ccatttgcgc taatgattgt cctcgtgata cggcgattgt
    67321 ttgcagtgct ttgatttttt ccagagttga tccaacttgt tcttctgaaa ggaatggtat
    67381 ttccttacga tgtgctctag aatcttcagg aataccatgt agataacgat ttgtcagcaa
    67441 cccttgagca agcggactga acgtgattgc ccctagccct tcttcttcta atactggaga
    67501 tagatcgtct tctagtagtg cacgagaaaa catattgtag cgcatctgat gaataatgaa
    67561 cggtgcttcc tttcttttta aaatttcagt catttttttt gtatcttctc cattatagtt
    67621 ggatatacca atatataatg cttttccttg tcttacaagt tgcatcaaag cttctgctgt
    67681 ttcttccaaa ggtgtatcag ggtctgggcg atgatgatag aagatgtcaa cataatctag
    67741 ccccattctt tggagacttt ggtcacaact tgaaatgatg gatttacgag agccccattc
    67801 cccatatggc ccttcccaca tatgataacc tgctttgcta gaaataatca gttcatcacg
    67861 gtaggcttga aaatcttctc taaaaatacg cccaaagttt ttctctgcag ctccagcagg
    67921 gcctccataa ttattcgcaa ggtcgaaatg ggtaattccc atgtcgaatg cgcctcttaa
    67981 aatctctctt tgattatgga taggatcata atcaccaaaa ttttgccata atccgagaga
    68041 taaaatcggt aatttcaaac ctgaatgacc ggatttacga tattccattt tttcataacg
    68101 tgcactatct gcaatataca atttttctcc tcctcatcaa atctttttaa aacgctttct
    68161 tttttctatt tttagtttac tacattttaa agaaaaggag ttaatcaaaa tgattttttc
    68221 gtatattttt ttgccatttg ttttttactc gttgatagat cagaaattga ttacgtgctt
    68281 gctggatttt tttataacgt ttcggttgtt ttttgtaaaa ttgctgatga tagttttctg
    68341 ctggccaaaa cgtaccggct ggttcgattt ttgtgacgat tggttggtgg aaagttcctg
    68401 aactgtctag tttctgtttt gattgttccg ctaattcttt ttgtcgttcg acttgataaa
    68461 aaatcactgg acgatattgc ttccctctat cttgaaattg tccaaatgta tctgtgggat
    68521 cagtaatttg ccaatagatt gtcagcaact cgctataact tatttctctt gtgtcataaa
    68581 tgatctccac agcttccaca tgacctgtgt aaccaccact tacttgatca taatttggtt
    68641 tttcaacatg tccgcccgtg tatcctgaca aaacagatag gatccctttt ttcgattcaa
    68701 acggctcgac catacaccag aaacatcccc ctgcaaatac tgcacgttct tgatagtcag
    68761 catcttgcat ggcatgcttg gaaaagtcat atttttgctc cttatcaaat ccttccgtaa
    68821 tcaataaata aaagtccatt acatcaggcg tcagattcat tcggatagct aatggacgta
    68881 gtgttgcttc tagttttgat aactgttctt taacggatat attttctctt atgtcattct
    68941 ttgtctgaat caatactttt cgttcccaat ctcgtgtatt cggattcaaa atcagattat
    69001 aaagtgtttg aatgaatatt tgttcctcat ccatagattc tcctcctttc cgaagaagac
    69061 aagctagtga ctttagctgt aatttatcat aaagtgagga aataagcgat tacttcatcc
    69121 ccctacagat ttttcgtata aaaaagagag aatccttatc gacagattct cctttttaat
    69181 caatttattt tataaatgaa aagttaattt accttttatg gaaaactatt ttaaaaaata
    69241 aacataaata tgcagttaat gtaacaagat aagaaataat agtcgttact gtataacttg
    69301 tgcttacttt aatatcagca actcctttga ctccgcttat ttgtaccaat ccattagcat
    69361 tcacctgctc ctttacaggt tgatgattta tttctacatt cgttcctaaa taagcatata
    69421 caggaagtgt gattacttgg ctcttctcat tagttactct atatatgata gaactaccag
    69481 ttgttttata aggaaggttt acccattgac tgccaatctt tgcttcgtgt tttattatat
    69541 gctccttatt ctccaatgaa gcgataggag aataatcata taaaaaataa tccattcctt
    69601 tagtggtata cgcctctact gtttctttgt ttacaacaat atgtcccggc tgagaaatta
    69661 ttttttgatt gaaatttttt ttactagaaa cagttaaagt gattaataac aaaacagtca
    69721 taaataaata aaatagttct cttgtcttat tgtatcttct atttttgaaa aatatcatcg
    69781 ttaatatcag agaagagaat attatttgca agcttaagat ccgccaagga aattgtaaca
    69841 atttggctgg cgtattttgc agaagatacc atggcaccaa actagttgta gctagccaag
    69901 tcaaacacgt accataataa atcttttttt ctatgtttgt taatttttta aataaaatca
    69961 ggggaataca taaagataac agaccaacta gtcccattga gtaagtagtt aagtcgttgt
    70021 tcatagcttc agtcagacta tcgaacacat tagatgcatg agcatataaa tttgtcacgc
    70081 taggtttgtt gattttttgg tagagcattt gttcaaacat cggataccaa aaagcactcg
    70141 tcagaaccaa tgtcattcct gccgcactaa ataatttttt aaatatagca ataaaatctg
    70201 cattcttgaa acctttagaa accaaacgaa taaaaataaa aatgataatt aatgctgtac
    70261 tcattaatgt tgatagtaca tggctgtaca ctagcaaact catactcaat gctagcttta
    70321 cagacggata cttttcttct tttccagaga tgatttgata agcataaaat aatacaggga
    70381 taacaagtgt tattgcgata gcttcggcaa ttgcggaacg atagtagatg tctactgtcc
    70441 gataagcaga gaaaatatag agacaactaa ataaaaatgc cgcttttttt tgttttaaaa
    70501 accgctcgcc ataatgataa gctacgagtg ttgtacacaa gttcaataac caaacgtaaa
    70561 taatatagct tacaatcaaa ttttttgtca tccaatataa cattacagct ggaaaaaaag
    70621 tcagaaaggg ataaaaataa tttacaccat atccagtata attgaatgtc tcataattaa
    70681 tcggactggt aaaaatagtc gataacccct tgattcgaga taagtgaaaa gaaagatctc
    70741 cagtggtata aatactctgt gtatttttga aaaagatgaa agaatatata ctactaacta
    70801 taattaatcc actcaatatt actacttttt tatttatttt cattcttctt gtttatctcc
    70861 tttttagtca catatcgaga atctttcaac ttattaggtg caaatcgttt aaaacttgaa
    70921 tacaccacta aacaataagt cagaatactg atccataaag ccatgagagc agttggagta
    70981 tattttgttg taatttctat cttattttct ccagcattta catccacatt cactaagcca
    71041 tgttttttat aagagtcatt tacgacttta ccgtttactt ttacctgtgt acctaaataa
    71101 gcatatactg gtactgtcac tttttgcttt ttgttgcttg ccactgtgta agtaatgtta
    71161 ttatctgcag ctttatactt ccctttttcc cacgattctc ctacttttac ttcatgtttt
    71221 tctaagtgag gcctttcttt tatcgcttct gttggggcgt aatcgtataa tcctcccata
    71281 tctgatgttg tataacgtgg gagtgtttct tcattgataa caatatgacc gtgttttgat
    71341 tggataactt ttgcgtaatt ttcttttgct gctatagacg tgacgagtag aaacaccaca
    71401 gtagcgccaa tgattaacca tgaatatttt ttattcatag tctttttcat aaaaacaagc
    71461 gttatgatca ttgaagaaaa aaagatttgg atacctaaga tacgccaagg aaactggagt
    71521 aagcttatcg gtgttttttg aaacaaaaac catggaaata aattagttac gcatacccat
    71581 gtagaaacta cgccaacata gatgattttt tcttttttct ccatttcttt cagtatgaat
    71641 aaaggaaaaa tcaacgcaac caatcctaat aaccccatag tgtatgttgt aatatcgtta
    71701 tttaacgctt gaatgatact atctgaaata gaaagtgctt ctagctgcaa atctgttttg
    71761 aagggactgt gtatttcttg ataattcatc tgctgcaaca taggatacca aaaatagcta
    71821 gttaatgcac aagtcaaaaa aacagccttt actaaattca cgaccacaaa ttttatatcg
    71881 ttttttattt gcttgcttaa aaacaaacga actataatca tacaagcaat caatgcacta
    71941 cacaatagtg tagttaagac atgagtatac acaagtagac ttatactgac agataaagcg
    72001 atgacttttt tttctctatt gaatatcaat ccataagcat aatataaaac agctgggata
    72061 atcgtaagag cgattgcttc tgcaattgcg gatcgataat agatatcaac tgttcgataa
    72121 gttgcaaaag tatataaaca actaaataaa aaagcagcat ctatcttttt aaaaaacttt
    72181 tctccataat aaaaactaat cagaatcgta caaatattta ataaccaaat atatagaaca
    72241 taacttaata ttaagttatt tgaaattcca taaaaaacga ctgcaggaat tattgtaaga
    72301 aaaggataaa aatagttcag tccatctcca taattgttaa atgttgtata gttgattggc
    72361 ccttcaaaaa tagaagataa ccctttgatt cgtgaaagat gaaaagataa atcacctgtt
    72421 gtataaattt cttgagcatt tctcataaaa tttactgaat ataaagtact aagtaagata
    72481 agaactacta aaagaaatgc ttttttactt attttcatag atttgttgtt ctcctttttt
    72541 aattctattt tcagtatact agaattgtct ttttttaata agttttgcgt taaatttttc
    72601 atagcactag tatgatatac tagaaaagaa tcaattttta ttacttttaa ataggagaaa
    72661 aaaatgaaaa aaaacaaaaa ccaaataata gatatcattt ttatgttatt tctaagtttt
    72721 gcttacgtca tccctctatt catgtctaag ggaatctatc atagtgtcaa tcaggatact
    72781 tactttcatt tatctcgaat cataggacta gataatgtat ggagttctcc agtaaacttc
    72841 aataacttcg atcatcatgg aacaatgatg aatatttttt atccatggct tactctttat
    72901 ccagcttttt tattttataa aatgatggga aatcttgttt taagttataa tatctactat
    72961 ttttttatca cttttttaac aatggtcgtt tcttattttt ctatgaaaca aataaaaaac
    73021 aatcgttata tttcgctgtt attttcgatt atttatacat tttccgccta tcgtgccatt
    73081 gacatattcc atcgtgcttc attgggagaa gctgtcgctt taacattttt gcctttgata
    73141 ctaatggggt gttacgaaat ttatattaga gattaccaaa aatggtactg gttgtctatt
    73201 ggcatgacgc tagtagtgta tactcattta ctttctgtcg ctatggtttc tgtatttatt
    73261 ggaggaacgt tgtttctttc tttttatttt tgggaccaaa aaatcgctag actcttaagt
    73321 cttttaaaag ctaccgtcct cacctttttt ttaagtgcgg gattcttaat tccttttatt
    73381 caacaatcta gagcacaaga attgaaagtc ccactgggaa aagaattaag tggtatggct
    73441 cctagtgata tgctgacaca tattttaaat aataactata acaactatac tattggatta
    73501 ttcttatttc tcggattgat tggtgctttt atttttataa aaaaattaac tgctgatgat
    73561 ttatttattt tctttcttgg tgtatttgtt cttttttgtt caaccaattt atttccttgg
    73621 caacttttta accatacacc agttaagagt ctacaatttg tctggcggtt aaatggtttt
    73681 tcttctttat ttatagccta tacgatgagt atagtgatct attatatttt ttctactaaa
    73741 aataattcat ggaaaataat ggtctttacg tttcttgcac taatattgca tctctcagga
    73801 gtaagcaatt ctatccatgc taacaaagat tcattaacac ttatagcacc tgaagacgct
    73861 gttgctatag ctgaaaatta caatcatact gattatgcaa acaaagagtc aatctatcat
    73921 cctgatatga ttaataatga tcaatattta ctaggtaacc aggaaataaa tccaacaact
    73981 cattttatga gtaacaaact tacaattgaa ttagataatt cgaataataa aaatactgtc
    74041 ttgacgacac ctatttatcg ttataaaggg caagtggctt ctattaatgg caaactagta
    74101 caaacaaaat tatctaagtt tggcacaact gaattaacta ttcctccagg tataaataaa
    74161 gtcgttatta cttatcagta cacaaaacta gcaattgctt caagatattt atctatcgtt
    74221 actttgattt tatttttact ttatcgattt actttttcta aacaaaaaca accgagaaga
    74281 gaagtacaac attctcacta gatttttatt taagaaagac tttttagctg tacacataaa
    74341 caggctaaaa agtctttttt tatatttctt taatttgctt catacattat ttaacatcga
    74401 tatttatact gtgaaacatg cgtttcatcc attatcaaag tatttttatc gataataaac
    74461 ttcatccaaa aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct
    74521 ttcaaatatt tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt
    74581 ccccatgata atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag
    74641 catttctccc tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga
    74701 ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc
    74761 attcagcttg tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc
    74821 tgaaggaatc acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct
    74881 tgaatggaag cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta
    74941 acgattcata aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc
    75001 tacttgtcct cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc
    75061 ttcatttgtc atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc
    75121 tccaagtctt tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt
    75181 tctcatattt tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt
    75241 gtagcacaaa atatagctcc ttgcttctct aagacagaag ctatacaaga aaactgtgga
    75301 aatatatatt tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa
    75361 tttgacggct caagttttac tgtttgagat gcaagatact caacctggca atgggcacat
    75421 aattaaagaa aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat
    75481 ttatagaagg aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca
    75541 gtccaaggta taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa
    75601 gaactggaaa caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg
    75661 tcgagacatt ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat
    75721 cacctatacc atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac
    75781 taacaaatgc agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag
    75841 accaaataag aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc
    75901 tcttcatttg atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac
    75961 tcagcacgcc taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc
    76021 tccaataaca attacctatg cttttaattg ttattataaa cgactttatc ttgatttaac
    76081 agaaacctct atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa
    76141 atactaaata tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat
    76201 tttttcccca cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact
    76261 tgacgaattt aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc
    76321 gacgatttgc atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa
    76381 taacagtgaa ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca
    76441 taaaaggact ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt
    76501 catcaaggaa atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga
    76561 tatggaataa gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat
    76621 atttgcggca gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa
    76681 aatcaatgga tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa
    76741 tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc
    76801 agcgattcca gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa
    76861 tgcaataatc acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt
    76921 tgtcgattct gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataagacgcc
    76981 aaggtaattc gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt
    77041 agataaaaca ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat
    77101 aggaacagta aaaattgaac ttacaataac tgctacaagt gcagaaagca atgttcccac
    77161 taaatataag atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat
    77221 tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa
    77281 caacgttccc aatattgaaa taaatgtcca tgtggggaaa aagataccaa acaatattcc
    77341 gactaaaata ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa
    77401 atgaccaccc tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg
    77461 tataattttc taaaaattct aacaatacaa acaaccattt cttgctaatt ttgtactttt
    77521 tctgtctaca cgttgttttt tgagatattc taagttagga tgttattaat gggcaactta
    77581 tcaggaaacg ttttcttatg tttttgtcca atttttttgt gaggtgttta ttcttgaaaa
    77641 aaataaagaa ggagagttat ggcttcctga caatctttgt cgcttgtata atgatggttt
    77701 tacagcgtat attgatttca gcagtcacta ttcctacata tgcaacaaac cctggtcgat
    77761 tctacatcta tacattttta caaacttccg ttgttattgg tgcaaatttg atcccacttt
    77821 acatgggtta ccaatatcaa aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt
    77881 ttgcctttat attcctaata gccacactag tttgcaatat ctttttttac gtacaagctg
    77941 gcagcttgaa tattcgtgat tattggctta tcctaacacc tatcagtcag aattacttta
    78001 cttatgctgt ttcttgtact ttgctttttt gtagtattcc ttatacagtt ggatttttaa
    78061 ataaattatc gaaaacaaca attaaatatc tactattatt tttaacaatt ggattagttg
    78121 gctgctctac tctatttaat aaagacccgt ttgcattaca gaatggacag agtattttat
    78181 ggatttttta tttattttta atgggttatg gtttgaaaga atggaactgg aaagaaaagg
    78241 tccgctataa gcttcctcaa gtatttatca gcttgattgt tttattcggt ttgattattt
    78301 tgatgacaca aatttcactc atcatcagag gaaatacaga tacagcgctg aggttttcta
    78361 aaccatattc tctcttcgca atgtattata ctttctcttc gtttttattt ttagaaatgc
    78421 tatctgataa attaggtata aaaggaagag gaaattttct tgctgtattt ttgattgtca
    78481 ctcaagttat atgtaatttc cctttagttt cttatcgcgt atctacattt atgaaacgtg
    78541 aatttccaaa ctcagggaag gcatggctga taaatatcgg gcagtttttt atttgatatt
    78601 tattagcagt gtccctccta gttatctttt tattttatat acaaaaaatc cgtttcattc
    78661 atcgtttcat cgaatacttc gcttttgatt cgattgaaca attaactcag cgttggaaaa
    78721 aaagaattca ttggcttttt gttcataaaa gttatttttt agttgcagca tttttttact
    78781 gctttacctt cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa gaaactatcc
    78841 aggttgcctt aaaaatattt actcagcgac aagcacccat gatcctgaca atcatcatta
    78901 ttatgggatt tttcttctta ctattgttga tttccaatcg tttttggtac gccttgactg
    78961 ctaccttgat cattaactta ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg
    79021 aagagcctgt ctttccttca gatttaaaaa tgctgaccgg attgtcagaa ctattatcga
    79081 tggttagccc agtcttatta atcgtgggcg gattgatcct tctcctatta ttgattacta
    79141 gtattattgt acaacgtaga ctacaaaaac agtattcctt gaagattcat tggaaacgtc
    79201 gatttatcag tatcgctgta ttattaggat gtttttcggg tgtctttttc attaatcaca
    79261 aaaattcgcc ttctttcttg ctgtttaatt tatttaaagt aaacaagact ttctttaacc
    79321 agcaaggcgc cgtaaaagat aacggaccag tcattcaatt cctaaacaat atcgacatta
    79381 aaatcatgga agaacctgct ggctattcga aagaaaaaat taatggaata atgaaaaagt
    79441 acaacacaaa ggcagatgaa atcaaccaaa caagaagtga ctggttaaac aatcagacga
    79501 tcgttctcaa tttaagtgaa agcttcagtg atcctgcacg tgtccctaat ctgacagtac
    79561 ctacagaccc tattccttac atcaaaagca ttataacaga aggaacagga ggattgatgt
    79621 tatccgtcgg ttatggtggc ggaacagcta acatggaatg ggaaggactg acaggcttga
    79681 gtatcagtaa cctgtctgct tcgttagtta caccttatac ccagctagtg gagcgtcaga
    79741 aaataagtcc aaatattacc gatctattcg atgaaaaaat agccatccat ccttttacag
    79801 ctgctcttta tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg ttctattatt
    79861 tagacagtcc ggataaatta acttatacgg ataaaataga acaaagccca cgcatatcgg
    79921 atgattcagc atacaaagaa accttgaaag ctttacaaag caatacggaa acgtcacaat
    79981 tcatccaatt atccactatg caaaaccata tgccttatga tgattactat agtgaattga
    80041 actacactgc tgaaggaact gctgtcattc ctaatagaag aaaagaatta caaacttata
    80101 tgcaaggcct tcactatacc gatacagcag taaaaaaatt catccaagaa atcgacaaaa
    80161 tcgataaacc gattaccttt gttttttatg gtgaccattt acccgcaatc tatagtggga
    80221 atgattcgaa aaaatatgga ttagaacagc accagacaga ttatttcatt tataacaaca
    80281 agtatagtcg tgaacaagca gctacgttaa ataaagaagt tgttgctcct aataactttg
    80341 gtgctatggc tcttgagcat gcgaatatca aagtaactcc ttactatgcg ttgttgacag
    80401 aagtatctaa tcatctacct gcattaacaa ttgatcctac agaaagcttg tctaatcgtt
    80461 ttaatggaaa acaggtcttt gttacagagg atagtcaaat tatccaagaa aaaaatttaa
    80521 ctgagaaaca aaaagaaatt ctagaagatt atcgtctgat acaatatgat cttgttgcag
    80581 gtaaccagta cagtgcaaca tgggcgacac aaaaaataaa agactaacaa ataaactgag
    80641 caaggggttg tgataaaaga catgtcacaa ccccttgttt atctcattaa tttgcttgtc
    80701 cataaacgat ttgattaaag ccatttttca attcattatt cgctgtctcg ttgaagtaaa
    80761 gataattatc gggattcccc tgattttgtt gtgtcaccaa tacttttggc tgattttgct
    80821 gaaacccgaa tagatagacg tgctttttca gatatggttc tgtctctgca tcggaataga
    80881 cggctgtgat ttgatagtct gcttgacctg tcccactttc tgaccattct gcagaaacag
    80941 gcactccacc aatagccatc ttccactccc cgttcaaaat agcttgaggg actttaagac
    81001 catataagtc cacactcatt tgattcgtat aacttttgta ttgttgtccc attgtttggc
    81061 cccatttggt cataaatgcc tctaattgtg cggatttgtc tgcattccac agtgtttgtt
    81121 caacttgttc gctgctttct gaagaggaag atgaagcctc tacagaagaa cttgaagtag
    81181 atgtttgagt tgtttcagtc gttttggaag actctgacga agtagaggta tctgctgttt
    81241 ctgtacttgc tgcagttgac ttgctctctg tgctagttgt tgtagtagtt gcaactgttg
    81301 ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta caaccagcta
    81361 acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa actcctttcg
    81421 attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat ttttcgtatt
    81481 ttctcatcaa tctttcaaat cttctaaaaa ctgagaagca cccgctgatg ttgtctgtaa
    81541 tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta cctcacgctt
    81601 tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc atccaaatct
    81661 tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt attgaaactt
    81721 aaaagttcaa caagcaattg ttgcaagcca tatttttctt ctttttcagc taatttgatt
    81781 gcccgtgtga taaagacatt ttggaaatca aaatcttttg caaagttttc caacattgtt
    81841 gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg catgacttct
    81901 tctaaagtag acggaacgat ttcattttca atcactaact catgagagat tttattgaac
    81961 catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg cgaagatgta
    82021 cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa atttgctaat
    82081 acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc ttgttttgtt
    82141 tcttgaacaa atttttcttc cactatattc attgatttac gcctctttta cttttgctga
    82201 ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt ggacttcatc
    82261 tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat tgcttacccc
    82321 aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag ctagtgtatc
    82381 taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga tagtcttatt
    82441 ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt tttcttcttt
    82501 gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca agatactagc
    82561 ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc cgttgagtaa
    82621 aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa cagttttctt
    82681 agccaagcgg taggcatgaa ccaacgattc aaaattagat tgcatcaaga caacgtcact
    82741 cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag ccgcagccaa
    82801 agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt gttcttggta
    82861 ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt catcaatgcc
    82921 taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca tcactaactg
    82981 ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc gaatcacatc
    83041 tgacacacct atgatttgag tgattttttt gtcgattgct acaataatga ccgagctgcc
    83101 tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc
    83161 ggcaatcaat ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc
    83221 ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa tgtctttctc
    83281 ctgagcataa tggatcacgg cgcgccccag aggatgatcg gataacgttt ctaatcgagc
    83341 cactaaagct aagctgtccc cttcatcctc tgtatagttc ttgatagtgg atacagcagt
    83401 agttcctttg gtcaatgtac cagttttatc aaagacaaaa gtatcgattt tggaaaaagt
    83461 atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac cgattcctgc
    83521 tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa ccaaaacagt
    83581 gattgctaaa cgaaaatcac gactaatcag accaacaacc aacgcaataa gtaaaacggt
    83641 tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt tggtatcttg
    83701 tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga ccttagttgc
    83761 ttcaatcgtc acgtagccat ctgctaaaat cgttccagag aagacttcat ctccactgat
    83821 tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcatacc ctttcccgtc
    83881 aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacga tatcgccttc
    83941 ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa cactagcagt
    84001 agatggggcc atctcggtca aagccttgat tgactcccgc gttttggtca atgttttttg
    84061 ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct tgtattctcc
    84121 gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga taactttgac
    84181 tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag aagctatggc
    84241 tagagcaatg ttatagaaca agacaaaacc agtaaatttt ccgataaaac caaaaataat
    84301 caacacagct gataaggcgg tgatatgatt tcgatgctct tggatccact tttgtaattt
    84361 catctttctc cctcttttcc tttcattctt acgagactaa gtatagatac ttatccatca
    84421 taaaaaattg accactatca agtttagtca aaaaggcggt tttcttttgg tagaatacac
    84481 tacaaaaaat agttagcaag aagactcact aaaagtgatc ttgccaacta ttgtcgtttc
    84541 tatcgaaatt ttcattgaat gattttcctg tacagcatac atcggagatc tatttcttta
    84601 tctaagattt ttatcttgct aattttttcc tttagtcttt caaatccacg tcgttcatag
    84661 aattgatacg tacagccact atctgtaaaa aggaatattt cttttccttt ctctcttttt
    84721 tcaaattctg ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg atttgcagct
    84781 aaaaaaatga tctctccatc tggcgaatgc ttctttaaat atgctgcaag catttgttca
    84841 tttgctgtct cgtaagctcc tttactttct ttagcaaata tattttgtaa aaaatcgaat
    84901 atcttaacat atatttgctg tccaaaagaa cgatgtttct ttttttctcc ttttatctcc
    84961 gccagcaaaa cacctgccaa ctcgtcctct gcatacaaag caagaatttg agttgctcga
    85021 ttcaactcca tatacaaaaa atatcttccg taaagattca gcagaagttc attttccata
    85081 tatctctcaa agttcattcc ttcaacagca aaatagatag cttttttgta atctttcttt
    85141 tctaagggtt taaaagttag tttcatcatt tcgcctcctc aacttcaatg attgtgttat
    85201 acttacatta taatagacac agtgtctact atctataatc aatcatctat aagtttgtcg
    85261 attaagcgac agctttgctt tgatatgtcg gataaggaga taggtaaatg aaaaagacag
    85321 atagacgcgt caaaaaaaca gaaaaagctt tggctgaaac actatccaca ttattagtaa
    85381 ataaaaagat tcaagcaatc actatacgcg aactaacaga aacggcagat gtacatcgtt
    85441 cgacttttta tacacattat aaggatatct atgatctata tgatcaatta gaaagcaact
    85501 tttttcggga tctaaatgaa attctctctt atgatccagc ccatagctat gaggaactat
    85561 acactcgctt gattgactat gtttacgcaa atactttttt atatcgctta tttatcctca
    85621 accatggtga tatttcgttt cagaagaaag ttaaacaagt tattgaagaa aactatctga
    85681 acatatggct atatgaagac aagaaaacga ttatcacaga agaaatgaga ttctttacta
    85741 cgtaccacgt ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc aattacaagt
    85801 actccaaaaa agaattatta gaactcttac agaaactgaa tgatcacata gagaaaatca
    85861 tgccttgaat aaaaacatac taagcttagt taagaagaac cgtaaccttc tcgtataaca
    85921 actgaatctt atttagccaa attgtttact gaagattttt tgagtaaaat ttctataatt
    85981 cctacttatt gaaaagtagt ttgtagtgct gcttctccct ctctaagtga tatatcttgt
    86041 tgtaccaatc aaattcacct catcattaat aaattacttt taggctaatt tattacaact
    86101 tataggttga agctgtaacc aatttatagt catattatat ttttatcatc cagtttattg
    86161 aattcttctc gtttacctca attcattttc aaggggaatc agctcatcat ctaacatttt
    86221 acgccatgct cgcttcattt cttgtgggta tttaaatggt ttcgcttctg gatgtttggc
    86281 tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc
    86341 acctggcata tattgcagat tagaagcatg taattttttc tcagaaacaa accactcatt
    86401 ggaaaagaca tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa
    86461 gatatcttca ttgctatcta gtgtaccttc atttctttgt gcttaaattc aaagataatt
    86521 ttctcaataa agatttttga ttccagtaag attcctttac tccttttaca acatagcgtt
    86581 gtcctttgtt ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc caattctcta
    86641 taaagaagat taagcttcat tattataata taaagtttaa atttggttta ttacttactt
    86701 tttcttattc tctagtaagc taatattgaa aaaaatgtaa tcagatcatt atttttatat
    86761 gttgtaactt cctgataaga aaactaatta aaagaaagag gtaactacat aaactaatta
    86821 aaaaagctaa cttcatagtt ggaggataaa tggaagaaac ttcaatgatt gtttctcctt
    86881 gtgttccttc aatcgacact agtcctatta tcaatctctc ttttttgtcc atttactttt
    86941 gcgactgttc cccaataatc aaatacagga agcgttattt tttgtccttt agattcatgt
    87001 actgtataga ctattttcga atccatcact tgataaggca tagtatgcca ttccttgcca
    87061 attcttactt catgattctg caaatgttct ttgtattgta atgcttccgt gggtgcatag
    87121 tcataaagtc ctccgggaag gagtgtcgtg taacgaataa gattttcttg attcattacc
    87181 aagtgatctc tctgagtgac aactttatgt tcatagtttg cttttgttgc agcagataca
    87241 gccaacagta ataaaacaat tgccgttata ttaactactt ttctttgctt agctgatgtg
    87301 ctcttcatga aaacgaacga taaaatcaac gagccaaaca gtacttgaaa gcctaagatt
    87361 cgccaaggaa attgaatcat ttgaatgggt gtattttgta ataaagacca tggaaaaaat
    87421 ttggtggata agagccaaga gactaccgca ctaaggtaaa tcactttttc tttgcgatta
    87481 aatcctttaa aaaagaagag tggacagatc aaagaaataa taccaatgat tcctaccgta
    87541 aaggttgtca ggtcattatt catcgcacca atcaaactat ccgagacatt taatgcttgg
    87601 tgttgtaagg tcgttttatc tggctcatta atcgattgaa ccatgatttg tcggaacatc
    87661 ggataccaaa agtatgctgt taaaagaatc gtcattccta ctgcctttga caactccaca
    87721 atcaaccgga tgctttcttt ttttcttgtt tcacttacta caaagccgcc taagactagt
    87781 aagccaacgg ctattacact cataaaagtg gaaagaacat gtgtataaat gagtaagctc
    87841 atactgactg ctaatggaat tgcacatggc tttttctcat aaatcagtgc gtaagcataa
    87901 tacataacta tcggtataac cgtaagtgca atcgcttcag caatagcaga acgatgataa
    87961 atatcaatcg tacggtaccc ataaaatgta tataaacagc taaatataaa agccgcatcg
    88021 attcttttta aaaattttag tccataatga aacatcagca tgatagtaca aacattcaat
    88081 agccaaacat ataagatata actaacgatt aaattattac ttatccaata aaaaatgact
    88141 gccggaaaaa aggtcaagta gggataaaaa taattcactc cgctcccata atgattaaac
    88201 gtcgtaaaat tgattggacc agaaaaaatt gaagataacc ctttgactct agcaaaatgg
    88261 aaggcaatat cccccgatgt agaaatagta tgaacatttc taaaataatt gattgaataa
    88321 agtccagcaa taattgctag gagaatggac aatattattt ttttctttac ttgcattact
    88381 taattatata ctcctttcat ggattgcttt atctagtata acttaaattt taaataaatc
    88441 gttatttatt tttactctca cttaaaaaag cactaaaatt ataagagagg gaggtaatgg
    88501 tctgaacacc aagaattgtt ccaacatcga attgcttaac taatgagttc aaagcttaat
    88561 tatgtggctc ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt
    88621 tcataaaaaa aacagccata tccgctttct tggatatgac tatttactac tactttacta
    88681 tctttcattc ttcttgtctc acttatgata aggcgctgcg tatcatgagt gaatgatatt
    88741 aggaaaactt cataaaataa atcttttata catttccact tctagatggt cattcatgtt
    88801 agaagaatca aactattttt ttactgtcgt aataatatta aaacactaga acaaatcaat
    88861 taaattccat aaaatataaa taatagacct agcatcatct gg
//
LOCUS       DACECS010000004        82999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.004, whole genome shotgun sequence.
ACCESSION   DACECS010000004 DACECS010000000
VERSION     DACECS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.004"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..561)
                     /locus_tag="IWO31_000256"
     CDS             complement(<1..561)
                     /locus_tag="IWO31_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0300706.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="IWO31_000257"
     CDS             complement(748..1398)
                     /locus_tag="IWO31_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300707.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IWO31_000258"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IWO31_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0300708.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="IWO31_000259"
     CDS             complement(2910..3908)
                     /locus_tag="IWO31_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0300709.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IWO31_000260"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IWO31_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0300710.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="IWO31_000261"
     CDS             complement(4924..5655)
                     /locus_tag="IWO31_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0300711.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="IWO31_000262"
     CDS             complement(5659..6924)
                     /locus_tag="IWO31_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0300712.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IWO31_000263"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IWO31_000263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0300713.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IWO31_000264"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IWO31_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0300714.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="IWO31_000265"
     CDS             12266..13768
                     /locus_tag="IWO31_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0300715.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="IWO31_000266"
     CDS             13770..14507
                     /locus_tag="IWO31_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300716.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="IWO31_000267"
     CDS             complement(14552..14725)
                     /locus_tag="IWO31_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0300717.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="IWO31_000268"
     CDS             14701..15366
                     /locus_tag="IWO31_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0300718.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="IWO31_000269"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="IWO31_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0300719.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="IWO31_000270"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="IWO31_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0300720.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="IWO31_000271"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="IWO31_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0300721.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="IWO31_000272"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="IWO31_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0300722.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="IWO31_000273"
     CDS             complement(20579..21745)
                     /locus_tag="IWO31_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0300723.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="IWO31_000274"
     CDS             complement(21761..21988)
                     /locus_tag="IWO31_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0300724.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="IWO31_000275"
     CDS             22472..23062
                     /locus_tag="IWO31_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0300725.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="IWO31_000276"
     CDS             23252..23965
                     /locus_tag="IWO31_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300726.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="IWO31_000277"
     CDS             complement(24080..25090)
                     /locus_tag="IWO31_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0300727.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="IWO31_000278"
     CDS             complement(25127..25609)
                     /locus_tag="IWO31_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0300728.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="IWO31_000279"
     CDS             25759..26127
                     /locus_tag="IWO31_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0300729.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="IWO31_000280"
     CDS             26592..27044
                     /locus_tag="IWO31_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0300730.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="IWO31_000281"
     CDS             complement(27168..28019)
                     /locus_tag="IWO31_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0300731.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="IWO31_000282"
     CDS             complement(28032..28817)
                     /locus_tag="IWO31_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0300732.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="IWO31_000283"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="IWO31_000283"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0300733.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="IWO31_000284"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="IWO31_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0300734.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="IWO31_000285"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="IWO31_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="IWO31_000286"
     CDS             34215..34388
                     /locus_tag="IWO31_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0300735.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="IWO31_000287"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="IWO31_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0300736.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="IWO31_000288"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="IWO31_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0300737.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="IWO31_000289"
     CDS             complement(37238..37549)
                     /locus_tag="IWO31_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0300738.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="IWO31_000290"
     CDS             complement(37546..37839)
                     /locus_tag="IWO31_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0300739.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="IWO31_000291"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="IWO31_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0300740.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="IWO31_000292"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="IWO31_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0300741.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="IWO31_000293"
     CDS             complement(39677..44035)
                     /locus_tag="IWO31_000293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0300742.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="IWO31_000294"
     CDS             complement(44247..45956)
                     /locus_tag="IWO31_000294"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0300743.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="IWO31_000295"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="IWO31_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0300744.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="IWO31_000296"
     CDS             complement(47453..48253)
                     /locus_tag="IWO31_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0300745.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="IWO31_000297"
     CDS             complement(48250..49062)
                     /locus_tag="IWO31_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0300746.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="IWO31_000298"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="IWO31_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0300747.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="IWO31_000299"
     CDS             complement(49817..50539)
                     /locus_tag="IWO31_000299"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0300748.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="IWO31_000300"
     CDS             complement(50675..51556)
                     /locus_tag="IWO31_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0300749.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="IWO31_000301"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="IWO31_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0300750.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="IWO31_000302"
     CDS             52796..53275
                     /locus_tag="IWO31_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0300751.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="IWO31_000303"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="IWO31_000303"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0300752.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="IWO31_000304"
     CDS             54576..54704
                     /locus_tag="IWO31_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0300753.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="IWO31_000305"
     CDS             54708..55253
                     /locus_tag="IWO31_000305"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0300754.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="IWO31_000306"
     CDS             complement(55707..57398)
                     /locus_tag="IWO31_000306"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0300755.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="IWO31_000307"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="IWO31_000307"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0300756.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="IWO31_000308"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="IWO31_000308"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0300757.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="IWO31_000309"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="IWO31_000309"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0300758.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61680..62381
                     /locus_tag="IWO31_000310"
     CDS             61680..62381
                     /locus_tag="IWO31_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0300759.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62990..64006)
                     /gene="tsaD"
                     /locus_tag="IWO31_000311"
     CDS             complement(62990..64006)
                     /gene="tsaD"
                     /locus_tag="IWO31_000311"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0300760.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64003..64467)
                     /gene="rimI"
                     /locus_tag="IWO31_000312"
     CDS             complement(64003..64467)
                     /gene="rimI"
                     /locus_tag="IWO31_000312"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0300761.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64474..65016)
                     /gene="rimI"
                     /locus_tag="IWO31_000313"
     CDS             complement(64474..65016)
                     /gene="rimI"
                     /locus_tag="IWO31_000313"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0300762.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65000..65824)
                     /gene="tsaB"
                     /locus_tag="IWO31_000314"
     CDS             complement(65000..65824)
                     /gene="tsaB"
                     /locus_tag="IWO31_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0300763.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(65913..66893)
                     /locus_tag="IWO31_000315"
     CDS             complement(65913..66893)
                     /locus_tag="IWO31_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0300764.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66917..68401)
                     /gene="galT"
                     /locus_tag="IWO31_000316"
     CDS             complement(66917..68401)
                     /gene="galT"
                     /locus_tag="IWO31_000316"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0300765.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68413..69402)
                     /gene="galE"
                     /locus_tag="IWO31_000317"
     CDS             complement(68413..69402)
                     /gene="galE"
                     /locus_tag="IWO31_000317"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0300766.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69650..69817
                     /locus_tag="IWO31_000318"
     CDS             69650..69817
                     /locus_tag="IWO31_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0300767.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69879..71690)
                     /locus_tag="IWO31_000319"
     CDS             complement(69879..71690)
                     /locus_tag="IWO31_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0300768.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71687..72052)
                     /locus_tag="IWO31_000320"
     CDS             complement(71687..72052)
                     /locus_tag="IWO31_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0300769.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72215..72610)
                     /locus_tag="IWO31_000321"
     CDS             complement(72215..72610)
                     /locus_tag="IWO31_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0300770.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72628..73590)
                     /locus_tag="IWO31_000322"
     CDS             complement(72628..73590)
                     /locus_tag="IWO31_000322"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0300771.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73590..73802)
                     /locus_tag="IWO31_000323"
     CDS             complement(73590..73802)
                     /locus_tag="IWO31_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0300772.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73823..74521)
                     /locus_tag="IWO31_000324"
     CDS             complement(73823..74521)
                     /locus_tag="IWO31_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0300773.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74541..75083)
                     /locus_tag="IWO31_000325"
     CDS             complement(74541..75083)
                     /locus_tag="IWO31_000325"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0300774.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75215..76219)
                     /locus_tag="IWO31_000326"
     CDS             complement(75215..76219)
                     /locus_tag="IWO31_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0300775.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76216..77205)
                     /locus_tag="IWO31_000327"
     CDS             complement(76216..77205)
                     /locus_tag="IWO31_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0300776.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77202..78008)
                     /locus_tag="IWO31_000328"
     CDS             complement(77202..78008)
                     /locus_tag="IWO31_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300777.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78174..79130
                     /locus_tag="IWO31_000329"
     CDS             78174..79130
                     /locus_tag="IWO31_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0300778.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79207..79725)
                     /locus_tag="IWO31_000330"
     CDS             complement(79207..79725)
                     /locus_tag="IWO31_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0300779.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79813..79962)
                     /gene="rpmG"
                     /locus_tag="IWO31_000331"
     CDS             complement(79813..79962)
                     /gene="rpmG"
                     /locus_tag="IWO31_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0300780.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80190..80636
                     /locus_tag="IWO31_000332"
     CDS             80190..80636
                     /locus_tag="IWO31_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0300781.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80831..82726)
                     /gene="asnB"
                     /locus_tag="IWO31_000333"
     CDS             complement(80831..82726)
                     /gene="asnB"
                     /locus_tag="IWO31_000333"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0300782.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23581 a        17672 c        14335 g        27411 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    61321 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt tattcatttt
    61381 ttattctgta tacactttca aaaaaatatt tattcattga aaaaattttt ttgaataaat
    61441 taaaaaaata aattatatta tcgttgttag cgtttactat atgtattatt gttgtttttt
    61501 atacacaaat cattcgcttc aaaacaaatt attcaatacc attgtatatt ttttacataa
    61561 taacaaatta tatttgtaaa gtattaaaac ttgtttctaa tgttggtatg ttaaaacttt
    61621 acaaattctt taaaaaaagt gacaataaat atagtgaaca ataaggagag gactttttaa
    61681 tgcgtgttaa taaaatcgat tttcactttt caaaactcag atatcaacct gattttgaac
    61741 atttctcgga tgaagagttc caacagatca aggaacatac tgttcttcgt tcttataaaa
    61801 aaggacagat ccttttcgac gatggtgatc ccagacaacg gttgtatatc ttgaccaaag
    61861 gactcgttcg tttagaacgt tatgatgaaa gtgcaactta ttactactat gactatgtga
    61921 atacaaataa cctttttcct atgtcaggaa tgtttggaga agagacctac acttatacag
    61981 ctcaagcgat gactgatatt gaaacattct accttcctgt ttcctactat gaaaaattag
    62041 cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa aatttctaaa gtagttgaaa
    62101 tacatgaatt gcgtatccaa atcggattga cctctagcgc ctttgaccgg gtaaaacaaa
    62161 atttattcat tttgaaagaa gaattgggcg taccaacaaa agatggtcat atcaccattc
    62221 cttatccgat caccttaaaa gaattagcag tgaatagcgg cacgacacgt gaaactgctg
    62281 gacaagtaat caaacaacta cgtgattccg ggctgttaga ttatgacaaa aagcgtttta
    62341 ctttctgtaa gacagctact actccagcta tgagagaata acgcgaacaa aaataagcag
    62401 tgaccctgat aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc
    62461 ggcagtttta gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa
    62521 tattcactgt ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc
    62581 gatctagatt attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg
    62641 aacggataat gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat
    62701 gtaaaaaata gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta
    62761 cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa
    62821 attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt
    62881 caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt
    62941 cctgggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata
    63001 aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca
    63061 catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca
    63121 ctagttctac ctcagggagt ttagctgcca aagcatcctg caatccttca cgcaaccctt
    63181 gattggccgc tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc
    63241 gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat
    63301 ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt
    63361 ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt
    63421 aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac
    63481 ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa
    63541 tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa
    63601 gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg
    63661 ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag
    63721 aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata
    63781 aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt
    63841 ctacgtgatg gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta
    63901 tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg
    63961 tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt
    64021 ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg
    64081 tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag
    64141 acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa
    64201 caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc
    64261 gacttcatct attactttcg agtatccaat aaagccacag atctggttat cttcagctaa
    64321 aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg
    64381 tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc
    64441 atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat
    64501 atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa
    64561 ccccaatttc cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat
    64621 cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat
    64681 tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa
    64741 cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta gatatagatg
    64801 aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt
    64861 atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag
    64921 tacgtaacga cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa
    64981 aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg
    65041 tctccaacca tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt
    65101 tctctgcaac tggttcaact aaagcaccta attgagccac agttacacca cttggaatat
    65161 cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga
    65221 atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga
    65281 acaaatcaga agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc
    65341 agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat
    65401 tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt
    65461 tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag
    65521 aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg
    65581 caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt
    65641 gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag
    65701 tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca
    65761 ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat
    65821 acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt
    65881 atcactccat tttcccacag cttttccatc ttttatttcg ttgattctcg aataatcaac
    65941 tctgtttcca caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc
    66001 gtctccactg ctagctctcc catccattcg gtataaactt ttacagtaga aagcgagggc
    66061 gttacatatt tcgctacact gacatcattg aagcccatca cagaaatatc ttctggtacc
    66121 actaaacctt tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca
    66181 aaaagcgcat caggaacatg cggattcgtt tttaaatatt gcttcactgc ttgatatcct
    66241 gactcgacag aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa
    66301 acttgttgaa atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat
    66361 tctgttccag caagcatagc gatatgttgc gctttatggg agacaaagtg ttcaatgact
    66421 ttttgaacac ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca
    66481 gaatctacca aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg
    66541 aattttccta gtgcgatcgt tccatctgcc ggaagatcac taatggaatc ccacgtttct
    66601 ttcaataaag taacgtttgc ttcttctgcc tttttttcga ttcctaggcg aatagataaa
    66661 taataaagat cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc
    66721 ttagtatttg ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa
    66781 attttttgct ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa
    66841 acagtagcag gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc
    66901 tcctcctttt ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt
    66961 catctcgctt gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt
    67021 tttgtacgac atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt
    67081 ctgcccacgg acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta
    67141 cttctactag ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca
    67201 acccaatgtt ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag
    67261 gatgttcttc tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc
    67321 ttcttcgtgc aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga
    67381 tagatactgt ttcatccgaa tatgctttcc atttttccaa tactgagtta gctgcttcaa
    67441 taagcaagtt tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc
    67501 ctgcgatgac ttgcggataa tcttctagtt tgataagttt ttctatcggt gcttttgcca
    67561 taggaaattc atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa
    67621 ttggtaagtc agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca
    67681 gtctagcaaa tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg
    67741 aatgttcatt gtaataagca taaggggaat attggaatcc ccagctttca ccgtctagat
    67801 tcattcgaat aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt
    67861 cgttttccat gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg
    67921 cgatttgttt tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat
    67981 actccgactc tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat
    68041 ttttcttact taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct
    68101 gtgcaaagaa tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt
    68161 caaaaatctc ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga
    68221 caagctgatc caataagtct acagattctt ccttaactgg tcttacttca atgctttcta
    68281 gagcatcttc tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca
    68341 tccatccgcc agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca
    68401 tatctcattc ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt
    68461 tttgataatc gctttgatat ctgtgtattc aggtttccag cccagaatct ctctcgcttt
    68521 ttcacttgaa gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg
    68581 aatttctttt cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt
    68641 gctgctgcct aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa
    68701 aatatgtgca gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg
    68761 tgtatcataa tcgtctccat aaacagccaa ggcttttctt tgaccaagag ctacttgtaa
    68821 aatgattggg acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt
    68881 tgctccggct acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca
    68941 ccacttcatc attttttcca tcatcaattt actttcacca tacggatttt taggattagc
    69001 tggtgtatct tctgtgattg gagactcttt cggctctcca taagttgctg cagtagaaga
    69061 gaatacgatt ttattcacgt tgaactcgtg catgacttca agtaagattt gcataccgta
    69121 tacgttgttg ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc
    69181 agcaaaatgg attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt
    69241 atcccggatg tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt
    69301 gagcaaatta tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg
    69361 cgaaccgata taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat
    69421 tagtaaaatt gtgtttgttg attctatata atttaaaaaa cgaagtttac ttttatggtg
    69481 aaaacaaaca aagtaaaatt tcgtaaattc atatggtaaa tttataataa aacatttagt
    69541 aaacattttc aagggttttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt
    69601 ctcttttgtt ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt
    69661 tgtatctgga atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc
    69721 ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa
    69781 ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga
    69841 agactttcta aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt
    69901 tgtgctattt tatctgcctg tctcagattc catggatctg tataaggtct agctactgct
    69961 gcagttagcc aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt
    70021 tcccctgaag catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga
    70081 taacttcctg tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt
    70141 tttatgacag gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa
    70201 tagcgactgc tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga
    70261 atatcaaatg gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct
    70321 ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc
    70381 catagtattt ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt
    70441 ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct
    70501 ttttccgctt cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca
    70561 ttctgatagg caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct
    70621 ggatgctttc cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc
    70681 tttggaatcg gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct
    70741 tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc
    70801 aggttttctt ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt
    70861 cctcgagggt aataagggag cccccttctt gatccactgt atacaatcgg ttccttgcct
    70921 gatggatgat agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta
    70981 aacaatccga cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta
    71041 ggcggtatct tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca
    71101 tttttctttg cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga
    71161 ccgcttgcta aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa
    71221 ccatcttttc ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga
    71281 aattcttctt gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta
    71341 gattgctgat ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt
    71401 accgtctgtt cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc
    71461 tgtgttacca atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa
    71521 acagaattca ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca
    71581 ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt
    71641 tctgctacac ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct
    71701 ccatttcgat aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat
    71761 aaacaaaagt aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct
    71821 tcttttcttc ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt
    71881 ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca
    71941 gatctaactg cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc
    72001 tatagccatc ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt
    72061 ccttctttct accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa
    72121 tgaacggcac aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga
    72181 ttcggttttt aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc
    72241 cagaccatcc gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta
    72301 atttatttgc tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt
    72361 cttttcgaat agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta
    72421 aaatatgccc agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca
    72481 ttccttctcg aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta
    72541 gatagacttc ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc
    72601 ataaaaccat gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc
    72661 aatgacacca gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg
    72721 cgggaaagta aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc
    72781 cgatactcct ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca
    72841 agctaaacct aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc
    72901 ttgagccatt tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt
    72961 cagttttgaa tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt
    73021 agcgctagat acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg
    73081 atccacagtg atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc
    73141 gccagcaacg atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc
    73201 gttttctcca gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat
    73261 agcgaatttg attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca
    73321 gtttaagtta tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc
    73381 catgccgatt ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat
    73441 tgattcaatg atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct
    73501 ccatttttgc tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc
    73561 taaatcaatc ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca
    73621 attcggtgct ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa
    73681 cggctatcat acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca
    73741 cccacataga taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc
    73801 atctttaagc acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa
    73861 tacatattac aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat
    73921 agcttgtctg ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc
    73981 caccgacatg tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca
    74041 caataaataa aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa
    74101 accctaagat aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca
    74161 gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat
    74221 aaatcaagaa aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca
    74281 gtatcagtga gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta
    74341 aacgccacca ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga
    74401 tctcaagtcg cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag
    74461 cataggtcca taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca
    74521 tgaaaacctc ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc
    74581 tccggttgcc attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga
    74641 aaattattta aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta
    74701 ttgtagataa tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct
    74761 gattgtggct caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca
    74821 taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt
    74881 tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct
    74941 atcacttttg ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct
    75001 ttttcctttt tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt
    75061 aatcttactt tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga
    75121 aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac
    75181 ctagaaatga ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc
    75241 tgataaaaag tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag
    75301 gtacttcccc acttggtaga atcactctag caaccatatc tgcactaagc agaaaaatgg
    75361 aaccgaacaa accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg
    75421 cagcatgagg agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc
    75481 ttgccaaagc aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta
    75541 atccttgcgc gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc
    75601 ctagcggaat taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac
    75661 cccagattga tcccgctaac catgcattca caaatccata attttcctta gataattgga
    75721 tcgtgcatag caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga
    75781 ttcgattcat acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag
    75841 ctaaaacacc acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa
    75901 agaaacctaa ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag
    75961 aatctgccaa ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag
    76021 ctcctgctaa taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca
    76081 aagaagatga ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg
    76141 taccaatcat taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg
    76201 tccaatatat ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc
    76261 aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt
    76321 aaaatccgcc aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg
    76381 atagtccgtt ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac
    76441 aggtccgact aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa
    76501 ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa
    76561 agatattgct tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa
    76621 tactgctaat tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc
    76681 ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat
    76741 agctacgcca gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa
    76801 accaaatacg agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa
    76861 atcagaagct ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt
    76921 gattcctagt aagcctgaat ctgctaaagg atttcttgtc accccttgca tcaatgcacc
    76981 gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt
    77041 tcgtatgatt tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc
    77101 tgctttttcc acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac
    77161 aaaacagacc aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc
    77221 tcttttggca acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca
    77281 atcgttgcat caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt
    77341 gtaccttctg tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa
    77401 gcatgattaa tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca
    77461 gtcaataact gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc
    77521 aagatcaaga tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt
    77581 ccaccagaca gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac
    77641 gcccactcaa ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg
    77701 tatcgtccgt aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt
    77761 gctaataatg caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta
    77821 ttgacaatga tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta
    77881 gattttccac ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca
    77941 gacatgtttt caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat
    78001 ctcgtcattt cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca
    78061 tttcgtcaag cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat
    78121 atcaggaata ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat
    78181 taaaaacaaa agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt
    78241 gctccccctc ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata
    78301 gtactcatac agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac
    78361 ggatcatcgg cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc
    78421 agtggactgt tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc
    78481 caacgatttc ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt
    78541 tgatttcttc ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc
    78601 caacttatgt tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg
    78661 gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca
    78721 tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt
    78781 gggtgaccaa caactcagcc tttatggtca gcaaaaatcg gtcaagtgga agaatcgtat
    78841 acgacgatct ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt
    78901 ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc
    78961 ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag
    79021 cagtgacaga caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac
    79081 ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa
    79141 aaaaagcatc ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca
    79201 tagattttat tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg
    79261 atgggcatat agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc
    79321 tgaaaaatca atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg
    79381 ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata
    79441 gatatcttct gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc
    79501 cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg
    79561 aagtgaaaga tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat
    79621 tgtatgttcg cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt
    79681 gccgatcaga ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta
    79741 tgattttaga tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga
    79801 actttaaaaa cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata
    79861 tttttgtttt tccaaacgat caggattgtt acgtttgttt ttgcttgtta gatagttacg
    79921 ttctttacaa gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt
    79981 tacaagaatc ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat
    80041 tgctagtgtt tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt
    80101 acttctcaaa caaaatcttt ttctttttgt ataaataccc ttacacttgt ttatatagat
    80161 tatgtttttt aataaagggg gatactaata tgccatggaa catgaaggac tttcctgcat
    80221 caatgaaaaa cttagataaa ttgacaagaa aaaaagcaat cgacatcgca aatgcattat
    80281 tagatgaagg gtaccctgat aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat
    80341 ggtatgaaga tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa aatccaagca
    80401 agacggatga acacgatacg aatcctcgtg ctggaaaact gcttgattca gatgtgatcg
    80461 tagaatatga ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc
    80521 attttgatac aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat
    80581 caactttgtt catttataaa aaagatggga caaaagaaaa agaaatcgat tattgaacat
    80641 atgtaaaggg gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt
    80701 tcctctgcca actccgaacc ataagtcata atatctataa aacgggaaga gctgtttttt
    80761 gaaattgttt cttatcactc agtgttaaac ggtaggagaa aacctctttt ttattgatat
    80821 ttgattttca tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc gtttcatcta
    80881 caaagtagac tttataccat gtcaagaaac tgaagattgt ccagattttt ctttgctctt
    80941 ctttacgtcc ttcgtgatgg tcatctagca acttcatgat tttttcttga tcaaaaaatt
    81001 cttttgcaaa atctgcttcg aataatgctt tgacttgttc gtagccgtgt tcttctttca
    81061 accaagcttt gattggcaca gggaatccta atttcacacg attcgaccat tcttccggaa
    81121 gatgtttgtt cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc aataaaaact
    81181 tacttggtat ttgttgagcc aatttcatga cttcgatatc caataaaggt actcggactt
    81241 ccaaagaact tgccatagaa agtttatctg cctttaacaa gatatctttt ggcatccatt
    81301 gatgaagatc gacatattgc atcttattga cttcatcttt gattccttct gttttctcgt
    81361 aatgaacagt catgatttca tccacagatg gtgctgtttg atattttggt gttaacactt
    81421 ctgatgcttc tgattcttcg aacactcgag catgaccgat aaaatattct tttgcaggag
    81481 caagtgattc atataaatgg atccgtccat ggaagttttt catttttccg attttacgtc
    81541 ctaaattata gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc acacggatga
    81601 attttgaatt cgtatggaat ccatacgttt ggtatcccgc aaacagttca tccgcccctt
    81661 ctccagattg gactacccgc acactttgtg aagcaagatt tgctaaaaag tacaaaggta
    81721 cgcaagaagg atttgaatca ggttcatcta agtgatactg gatcaaaggg aatgctttga
    81781 acgacatgtc tccgtcgatc actgctgctg tattatctaa gtccaatagt tcggttagtt
    81841 ttctggcttc aatcgcttca ttataggttt tatcatcaaa cccgattgag aaagaatgat
    81901 ctggtttcaa tacagaggtt acataactgg aatcaacccc gctggataag aaagaaccaa
    81961 cttctacatc actgaccgta tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt
    82021 cgatccattc ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc caatattgct
    82081 gaatgtctat ttttccatct ttatacgtat aataatggcc ttcaggcaaa cgatagacat
    82141 ctttgaagaa tgtttcgccg tttaatggag aatattggaa agtcatataa ggtttcaacg
    82201 cttctttgtt caattctttg ttaaaatcag gatgaggcag aaaacttttg atttctgaac
    82261 caaacataaa tgttccgttc atttctgcat aataatatgg tttgatccca aagtggtctc
    82321 ttgcaccgaa taactcattc ttttcattat cccaaatagc aaaagcaaac attccgcgga
    82381 tcttttgcaa taattctgtt ccccattctt cgtaaccatg caaaaggact tctgtatctg
    82441 catgtgtttg aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc tggtaattat
    82501 agatttcgcc attaaaaata atgatctttg tccgatcttc attgtaaata ggctgagtac
    82561 cgccctctaa gtcaataata cttaatcttc tgaatcctaa agcaacatgt ttatcgatat
    82621 attctcctga actattaggt ccacggtgaa cgattcgatc catcattgta ttaataattg
    82681 tttttttgtt gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa ctttcacatc
    82741 ctaatataaa attactgttt atttcattca tatgcatctt atcatagaat aaatagcgga
    82801 cgaatatttt ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt
    82861 tacagaaaat aaagtcccaa tatctaattt accatgtttt cttttttgag gctaccttat
    82921 tctcttcgtg ttaaaaaaat attagtggat gctatttcca aaaaaaccag caaaaaaaga
    82981 cacgacctaa agatcgtgt
//
LOCUS       DACECS010000005        75330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.005, whole genome shotgun sequence.
ACCESSION   DACECS010000005 DACECS010000000
VERSION     DACECS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.005"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            163..393
                     /locus_tag="IWO31_000334"
     CDS             163..393
                     /locus_tag="IWO31_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0300783.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            490..1791
                     /gene="murA"
                     /locus_tag="IWO31_000335"
     CDS             490..1791
                     /gene="murA"
                     /locus_tag="IWO31_000335"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0300784.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1804..1980
                     /locus_tag="IWO31_000336"
     CDS             1804..1980
                     /locus_tag="IWO31_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0300785.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2115..2411
                     /gene="yidD"
                     /locus_tag="IWO31_000337"
     CDS             2115..2411
                     /gene="yidD"
                     /locus_tag="IWO31_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0300786.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2475..2942
                     /locus_tag="IWO31_000338"
     CDS             2475..2942
                     /locus_tag="IWO31_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0300787.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3027..3911
                     /locus_tag="IWO31_000339"
     CDS             3027..3911
                     /locus_tag="IWO31_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0300788.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4028..4261
                     /locus_tag="IWO31_000340"
     CDS             4028..4261
                     /locus_tag="IWO31_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0300789.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4349..4429
                     /locus_tag="IWO31_000341"
     tRNA            4349..4429
                     /locus_tag="IWO31_000341"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4383..4385,aa:Tyr,seq:gta)
     gene            4436..4507
                     /locus_tag="IWO31_000342"
     tRNA            4436..4507
                     /locus_tag="IWO31_000342"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4468..4470,aa:Gln,seq:ttg)
     gene            5022..5729
                     /locus_tag="IWO31_000343"
     CDS             5022..5729
                     /locus_tag="IWO31_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0300790.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5789..6433)
                     /locus_tag="IWO31_000344"
     CDS             complement(5789..6433)
                     /locus_tag="IWO31_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0300791.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6560..6847
                     /locus_tag="IWO31_000345"
     CDS             6560..6847
                     /locus_tag="IWO31_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300792.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6955..7275
                     /locus_tag="IWO31_000346"
     CDS             6955..7275
                     /locus_tag="IWO31_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0300793.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7356..8669
                     /locus_tag="IWO31_000347"
     CDS             7356..8669
                     /locus_tag="IWO31_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0300794.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8666..9385
                     /locus_tag="IWO31_000348"
     CDS             8666..9385
                     /locus_tag="IWO31_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0300795.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9513..10064
                     /gene="raiA"
                     /locus_tag="IWO31_000349"
     CDS             9513..10064
                     /gene="raiA"
                     /locus_tag="IWO31_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0300796.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10286..12820
                     /gene="secA"
                     /locus_tag="IWO31_000350"
     CDS             10286..12820
                     /gene="secA"
                     /locus_tag="IWO31_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0300797.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12946..14047
                     /gene="prfB"
                     /locus_tag="IWO31_000351"
     CDS             join(12946..13008,13010..14047)
                     /gene="prfB"
                     /locus_tag="IWO31_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0300798.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14152..14838
                     /gene="ftsE"
                     /locus_tag="IWO31_000352"
     CDS             14152..14838
                     /gene="ftsE"
                     /locus_tag="IWO31_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0300799.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14822..15715
                     /locus_tag="IWO31_000353"
     CDS             14822..15715
                     /locus_tag="IWO31_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0300800.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15867..17315
                     /gene="cls"
                     /locus_tag="IWO31_000354"
     CDS             15867..17315
                     /gene="cls"
                     /locus_tag="IWO31_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0300801.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17652..18509
                     /locus_tag="IWO31_000355"
     CDS             17652..18509
                     /locus_tag="IWO31_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0300802.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18624..19544
                     /gene="pstC"
                     /locus_tag="IWO31_000356"
     CDS             18624..19544
                     /gene="pstC"
                     /locus_tag="IWO31_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0300803.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19544..20428
                     /gene="pstA"
                     /locus_tag="IWO31_000357"
     CDS             19544..20428
                     /gene="pstA"
                     /locus_tag="IWO31_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0300804.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20443..21249
                     /gene="pstB"
                     /locus_tag="IWO31_000358"
     CDS             20443..21249
                     /gene="pstB"
                     /locus_tag="IWO31_000358"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300805.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21265..22023
                     /locus_tag="IWO31_000359"
     CDS             21265..22023
                     /locus_tag="IWO31_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300806.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22036..22713
                     /gene="phoU"
                     /locus_tag="IWO31_000360"
     CDS             22036..22713
                     /gene="phoU"
                     /locus_tag="IWO31_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0300807.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22951..23982
                     /locus_tag="IWO31_000361"
     CDS             22951..23982
                     /locus_tag="IWO31_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0300808.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23979..24746
                     /locus_tag="IWO31_000362"
     CDS             23979..24746
                     /locus_tag="IWO31_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0300809.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24846..26414
                     /gene="liaX"
                     /locus_tag="IWO31_000363"
     CDS             24846..26414
                     /gene="liaX"
                     /locus_tag="IWO31_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0300810.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26714..27031
                     /locus_tag="IWO31_000364"
     CDS             26714..27031
                     /locus_tag="IWO31_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0300811.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27031..27381
                     /locus_tag="IWO31_000365"
     CDS             27031..27381
                     /locus_tag="IWO31_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0300812.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27548..28486
                     /locus_tag="IWO31_000366"
     CDS             27548..28486
                     /locus_tag="IWO31_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0300813.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28501..29331
                     /locus_tag="IWO31_000367"
     CDS             28501..29331
                     /locus_tag="IWO31_000367"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0300814.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29375..30400
                     /locus_tag="IWO31_000368"
     CDS             29375..30400
                     /locus_tag="IWO31_000368"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0300815.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30415..31353
                     /gene="galU"
                     /locus_tag="IWO31_000369"
     CDS             30415..31353
                     /gene="galU"
                     /locus_tag="IWO31_000369"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0300816.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31478..31918
                     /locus_tag="IWO31_000370"
     CDS             31478..31918
                     /locus_tag="IWO31_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0300817.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31921..32523
                     /locus_tag="IWO31_000371"
     CDS             31921..32523
                     /locus_tag="IWO31_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0300818.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32582..33688)
                     /locus_tag="IWO31_000372"
     CDS             complement(32582..33688)
                     /locus_tag="IWO31_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0300819.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33898..34899
                     /gene="ccpA"
                     /locus_tag="IWO31_000373"
     CDS             33898..34899
                     /gene="ccpA"
                     /locus_tag="IWO31_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0300820.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35236..37581)
                     /locus_tag="IWO31_000374"
     CDS             complement(35236..37581)
                     /locus_tag="IWO31_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0300821.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37860..38762
                     /locus_tag="IWO31_000375"
     CDS             37860..38762
                     /locus_tag="IWO31_000375"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0300822.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38975..40750
                     /locus_tag="IWO31_000376"
     CDS             38975..40750
                     /locus_tag="IWO31_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0300823.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40816..42402)
                     /locus_tag="IWO31_000377"
     CDS             complement(40816..42402)
                     /locus_tag="IWO31_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0300824.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42653..42877
                     /locus_tag="IWO31_000378"
     CDS             42653..42877
                     /locus_tag="IWO31_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0300825.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43019..44770
                     /locus_tag="IWO31_000379"
     CDS             43019..44770
                     /locus_tag="IWO31_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0300826.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44770..46554
                     /locus_tag="IWO31_000380"
     CDS             44770..46554
                     /locus_tag="IWO31_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300827.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46880..47731
                     /locus_tag="IWO31_000381"
     CDS             46880..47731
                     /locus_tag="IWO31_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0300828.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47785..49536
                     /locus_tag="IWO31_000382"
     CDS             47785..49536
                     /locus_tag="IWO31_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0300829.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49540..49710
                     /locus_tag="IWO31_000383"
     CDS             49540..49710
                     /locus_tag="IWO31_000383"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300830.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49751..50653)
                     /locus_tag="IWO31_000384"
     CDS             complement(49751..50653)
                     /locus_tag="IWO31_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0300831.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            50883..52286
                     /locus_tag="IWO31_000385"
     CDS             50883..52286
                     /locus_tag="IWO31_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0300832.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            52286..53719
                     /gene="ascB"
                     /locus_tag="IWO31_000386"
     CDS             52286..53719
                     /gene="ascB"
                     /locus_tag="IWO31_000386"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0300833.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53864..54790
                     /gene="trxB"
                     /locus_tag="IWO31_000387"
     CDS             53864..54790
                     /gene="trxB"
                     /locus_tag="IWO31_000387"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0300834.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55004..56731
                     /locus_tag="IWO31_000388"
     CDS             55004..56731
                     /locus_tag="IWO31_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0300835.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            56804..57097
                     /locus_tag="IWO31_000389"
     CDS             56804..57097
                     /locus_tag="IWO31_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0300836.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57191..58051)
                     /locus_tag="IWO31_000390"
     CDS             complement(57191..58051)
                     /locus_tag="IWO31_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0300837.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            58143..58571
                     /locus_tag="IWO31_000391"
     CDS             58143..58571
                     /locus_tag="IWO31_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0300838.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58733..58909
                     /locus_tag="IWO31_000392"
     CDS             58733..58909
                     /locus_tag="IWO31_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0300839.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58936..59382
                     /locus_tag="IWO31_000393"
     CDS             58936..59382
                     /locus_tag="IWO31_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0300840.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59538..60917)
                     /gene="brnQ"
                     /locus_tag="IWO31_000394"
     CDS             complement(59538..60917)
                     /gene="brnQ"
                     /locus_tag="IWO31_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0300841.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61623..62594
                     /locus_tag="IWO31_000395"
     CDS             61623..62594
                     /locus_tag="IWO31_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0300842.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62618..64810
                     /locus_tag="IWO31_000396"
     CDS             62618..64810
                     /locus_tag="IWO31_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0300843.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            64827..65303
                     /gene="ybeY"
                     /locus_tag="IWO31_000397"
     CDS             64827..65303
                     /gene="ybeY"
                     /locus_tag="IWO31_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0300844.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65281..65682
                     /locus_tag="IWO31_000398"
     CDS             65281..65682
                     /locus_tag="IWO31_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0300845.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            65697..66596
                     /locus_tag="IWO31_000399"
     CDS             65697..66596
                     /locus_tag="IWO31_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0300846.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66739..67551
                     /gene="recO"
                     /locus_tag="IWO31_000400"
     CDS             66739..67551
                     /gene="recO"
                     /locus_tag="IWO31_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0300847.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            67937..68854
                     /gene="glyQ"
                     /locus_tag="IWO31_000401"
     CDS             67937..68854
                     /gene="glyQ"
                     /locus_tag="IWO31_000401"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0300848.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68856..70931
                     /locus_tag="IWO31_000402"
     CDS             68856..70931
                     /locus_tag="IWO31_000402"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0300849.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            71316..72095
                     /locus_tag="IWO31_000403"
     CDS             71316..72095
                     /locus_tag="IWO31_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300850.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            72136..73080
                     /locus_tag="IWO31_000404"
     CDS             72136..73080
                     /locus_tag="IWO31_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0300851.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            73096..73875
                     /locus_tag="IWO31_000405"
     CDS             73096..73875
                     /locus_tag="IWO31_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0300852.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            73888..74586
                     /locus_tag="IWO31_000406"
     CDS             73888..74586
                     /locus_tag="IWO31_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0300853.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            74614..>75330
                     /locus_tag="IWO31_000407"
     CDS             74614..>75330
                     /locus_tag="IWO31_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0300854.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEIS"
BASE COUNT        24674 a        13311 c        16122 g        21223 t
ORIGIN      
        1 cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa aaatgtaagg
       61 ggattcctat atttttaagt atacttgttt ttccttttat acattttatg gtattatcat
      121 tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt atacggaatt
      181 gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt ctggtcttta
      241 cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat tagaatggca
      301 atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt agaagttatc
      361 gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac tattttaaaa
      421 gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga attttttgga
      481 gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg aacagtaaga
      541 attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt agcagaagaa
      601 ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat gaatcaagtg
      661 attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt aacaatcgat
      721 gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca
      781 atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc tatgcctggt
      841 ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca agctttagga
      901 gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt gatcggtaac
      961 acgatttatt tagatttccc tagtgtaggt gctactcaaa atatcatgat ggctgccgta
     1021 aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat cgttgattta
     1081 gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga aacaatgcgt
     1141 attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa
     1201 gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat tgcagatgct
     1261 atctctgaac ataaccgccc attgatttct aaattgatcg aaatgggtgc agaaatcatt
     1321 gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac agatgtaaaa
     1381 acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc tatccaatta
     1441 gttgcagaag gtacaagtgt agtgactgaa acagtgtttg aaaatcgttt ccaacattta
     1501 gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat
     1561 ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc agcagcagca
     1621 ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt gaattatcta
     1681 gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt
     1741 gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta aggaagtggc
     1801 tgaatgagta accaacgata tatcttatta actttgataa agattttagt ggtgatctta
     1861 ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat cggtggcggc
     1921 aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt ttttcattga
     1981 taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca gaacatgaca
     2041 aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt aaaagaaaag
     2101 aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat cagcgcttta
     2161 tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat tatatgataa
     2221 aagcaattca aacccatggt gcactaaaag ggacggtaat ggggactgca cggatcttac
     2281 gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc caattaacgc
     2341 gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca
     2401 ctgagcgata aagaaaaaga ggaaaattat tgtcaaagta acaactccgc aactacttat
     2461 atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg tccttgttat
     2521 tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg agtggctgga
     2581 tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg gaaaaagaat
     2641 acgaaatcgt caatgtagca gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat
     2701 tacttgagac agcttttcag aagctttcac aactcgcttc cagtcaaact cagatcatca
     2761 tacgaaccgg cagtactagc agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg
     2821 agattggtcg agtcaaagac tattttatcc atcattactc tgagcccatt tttgaaaatg
     2881 gagatcaatt aagggatcaa gtaattttag caattcactt acctaaaaat agctttaact
     2941 aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt acaatagctt
     3001 tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt ggtggatcgg
     3061 ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa tttgcaacgc
     3121 ctgaatcttg ggaagaaatg aaagcgcaat tatttcaagt ttataaaaaa atactcgttt
     3181 cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat gagaaacagg
     3241 tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc cgagatttgg
     3301 aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc ggaatggctg
     3361 aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg gcaggaactg
     3421 ggattggcgg ggcgttgttc acgaaaggac aaatcaataa aggggcacat ctttatggtg
     3481 gagaatttgg gttgatgttc ttaaatggcg ataagacatt cagtaaactt ggtacagcgg
     3541 tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaattct tataccggaa
     3601 aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa gttgagagtt
     3661 tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat ccagaagtga
     3721 tcgtattagg cggcggtgtt tctgcaaaag aagggctcat tgatgagatc aaaagccgaa
     3781 tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt ttattatgtg
     3841 aatacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag
     3901 gggagaatta agagctttag agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat
     3961 aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata tctgagaggt
     4021 gaaaatgatg tcaaaaaaaa ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga
     4081 tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa tgattggtga
     4141 gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag attttggaaa
     4201 agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc tgcacgaata
     4261 aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat taggtattat
     4321 aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa acgcgacgga
     4381 ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat ttcattgggg
     4441 tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct
     4501 accccagtat accacaatcg taagctctcg ttagttcgag agctcttttt tttgctttgc
     4561 taccttgttc cttgaagatt ctgctgattt aagatagagt taagttaact acaagaattg
     4621 gagggatcca catgaaatgg aaagaaaaag tagacgaagc agctgaaaag ttatatgatt
     4681 tggtcaaatc agaaaaatac aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg
     4741 tccaggtaaa atcaaaacgc ccaacgaatc atacaaaaaa atggatggct aaaaaccgct
     4801 aataataaca aaagatccat aagtcggcat tttcatcgat tttagggtct tttgttattt
     4861 tctaggttga acatatctaa aaaattgttt ctgacaatag gagtaggaag aacgatgtgt
     4921 gtctaaatcc tctgttgatg gatagaaacc tttatctttt tcagttgtga atcaggtata
     4981 taccagttat aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat caattacctg
     5041 tatatataca gattcatgac caattaaaaa atgaaatcga acaaggtata tggaaagtcg
     5101 gagaccgtct tccttcagaa agagaattag cagtgaaatt caatgtcagc cgtatgacgt
     5161 tgagacaagc aattcaaaac ttggcagatg aaggaatctt agaaagaaag atcggttcgg
     5221 gtacatatgt tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca agttttacag
     5281 aaattatgga atcgcaaaat cgagtaccgt ctagtcggac aatctcttat ttcttgactt
     5341 cgccaagctc aagtgaaatg gaaaaactaa acttgagtaa agatgacacg atcttgcgaa
     5401 tggaacgtat tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc agtgtgccac
     5461 aaaagctgat cgatggctac agcaaagcag agattactga atcgttgtat cgcacgctag
     5521 aagaaaaagg cgggaaaaag atcggtccag cgaatcaaac gattactgct gtgttggctt
     5581 ctgagaaaat tgctgactat ttagaggtaa aacgaggaga tgcagttctt cgtttgcgcc
     5641 agatttctta ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa
     5701 atcgttttga gttttatttg gaaaaataaa aaacaggtga tccctgattc cttacttaga
     5761 aataaaagag aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat
     5821 aagaaacagg accctcttca aattggactt gtccgttcag cagctcaatg atggattctt
     5881 taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt
     5941 ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg
     6001 gatacgtgat catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
     6061 acaatgcttg agagacagca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
     6121 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
     6181 cttccaacat cggtacgcca gccgttccac ttggttctcc atcatcgctg cttcgctgga
     6241 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
     6301 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
     6361 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
     6421 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
     6481 ctaggttgtt tcaacactgt aacggttgcg caatcgctgt agtatgcgat aataaagaca
     6541 aaggtaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
     6601 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
     6661 gtttcccagt aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
     6721 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
     6781 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
     6841 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
     6901 taacagacgc gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct
     6961 aaaaaaacaa tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
     7021 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
     7081 gacgcagata acaatcttgc aaataaagat gtagatgtac tgttattagg acctcaagtt
     7141 cgctttatga aagctgattt tgaaaaacgt ctggaaccaa aaggcattcc tttagacgtc
     7201 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
     7261 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
     7321 cttgtttttt cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc
     7381 ttctgacgag agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga
     7441 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
     7501 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
     7561 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
     7621 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atcgcaaaga
     7681 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
     7741 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
     7801 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
     7861 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
     7921 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
     7981 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
     8041 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
     8101 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttgcgcttt catcggcgtt
     8161 tacttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
     8221 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
     8281 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
     8341 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
     8401 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
     8461 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
     8521 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
     8581 actttttcta tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa
     8641 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcgtcca
     8701 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
     8761 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
     8821 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
     8881 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
     8941 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
     9001 actagcaggc acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat
     9061 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
     9121 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
     9181 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
     9241 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
     9301 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
     9361 ccgaaccatt tctttagcaa gatgaattag atgtggaaat ttgatagcga tttaatttgc
     9421 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
     9481 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
     9541 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
     9601 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
     9661 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
     9721 cttctccaga tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc
     9781 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
     9841 ctactgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
     9901 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
     9961 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    10021 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    10081 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    10141 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    10201 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    10261 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    10321 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    10381 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    10441 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    10501 agcaaaacgt gtattggggt tatatccaca ccacgtacaa ttgatgggtg gaatcgtgct
    10561 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    10621 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    10681 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    10741 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    10801 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    10861 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    10921 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    10981 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    11041 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    11101 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    11161 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    11221 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    11281 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    11341 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    11401 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccagat
    11461 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    11521 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    11581 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    11641 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    11701 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    11761 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    11821 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    11881 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    11941 acgaatcaaa tctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    12001 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    12061 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    12121 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    12181 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    12241 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagtattac
    12301 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    12361 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    12421 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    12481 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    12541 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    12601 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    12661 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    12721 aaaacagcca atccatgtga acaaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    12781 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    12841 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    12901 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    12961 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gcttcaggag gtctctttga
    13021 cttagaccag ctggaagaag acattgcgga agcagaagca aaaatggctg aacctggttt
    13081 ttgggatgat agcgaagctg ctcaaaaagt gatcagtgaa aacaatgcaa ataaagaaac
    13141 ttatgatcaa ttccattctc ttgctgaaga actggaagaa atcgaattga tggtggagat
    13201 gttgcaggaa gaacctgatg cagccatgca agaagaagca gaaacacgta tcaatgcgct
    13261 agatgaaaaa atgaagacat atgaattatc tatgttgtta gatggtcctt atgatcgaaa
    13321 caacgccatc gttgaattgc atccaggggc tggcggaacg gagtcccaag attggggcag
    13381 tatgctttta cgtatgtata cacgctgggc agagcagcat gggtatcaag tagagacact
    13441 agattatcaa gcaggtgatg aagcaggaat caagagtgtg accctactga tcaaaggaca
    13501 caacgcattt ggttatctga aatcagaaaa aggtgttcat cgccttgtac gtatctcgcc
    13561 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    13621 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    13681 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    13741 gcctacagga acagtagtag ctagtcaggc acaacgttca caattgaaaa accgtgagca
    13801 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    13861 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    13921 tgttttccat ccatattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    13981 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    14041 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    14101 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    14161 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcaatt
    14221 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    14281 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    14341 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    14401 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    14461 atgcaagtta tcggcagaaa acctcgggat atcaaaaaac gagtaatgga agtgctggac
    14521 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    14581 caacgtgtct ctattgctcg ggcaatagtg aatacgccta aagtacttat cgcggatgaa
    14641 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    14701 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    14761 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    14821 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    14881 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacat tgactttagt
    14941 cggtgtattc atggcagtta tcatgaacgc aactaaatta gctcaagata tagaaggaaa
    15001 cgttgatgtc tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    15061 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    15121 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    15181 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    15241 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    15301 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    15361 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    15421 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    15481 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    15541 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    15601 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    15661 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    15721 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    15781 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    15841 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    15901 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    15961 ctcaaacatg ggcatgggtg ctcgttttga tgttcattcc agttgtagga tttattttat
    16021 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    16081 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    16141 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    16201 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    16261 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    16321 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    16381 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    16441 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    16501 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    16561 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    16621 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    16681 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    16741 tttttccgtc cattcactct actggtgata tagctgttca aatcgtgaca agcggaccag
    16801 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    16861 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    16921 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    16981 tcgtttactg ggcaacgtat tctttcgctg cagagctaat cgagtatgga gcaaggatcg
    17041 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    17101 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    17161 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    17221 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    17281 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    17341 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    17401 tggagaaagc gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa
    17461 agcaagaaat aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat
    17521 agaaactttt aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg
    17581 attttgattc gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa
    17641 ggagatttct aatgaaaaaa cttaaattat tcttaccaat aataggatta gctattcttt
    17701 tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg
    17761 ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat
    17821 tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag
    17881 tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat
    17941 tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg
    18001 taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga
    18061 gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa
    18121 cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac
    18181 aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg
    18241 ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga
    18301 cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa
    18361 gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac
    18421 aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg
    18481 attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa
    18541 cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa
    18601 acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa
    18661 aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg
    18721 tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc acctttttta
    18781 ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg
    18841 gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga
    18901 cctttttatc tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg
    18961 agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta
    19021 ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta
    19081 tttttggcgg gactgggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct
    19141 tgccgactgt cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg
    19201 aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag
    19261 cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag
    19321 ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacaggt cttactactc
    19381 ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag
    19441 ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta
    19501 atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc
    19561 agataaaatt gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc
    19621 agctttacta ctttatatct tagttcgggg aataccgcac atttcatggt ccttcttgac
    19681 cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat
    19741 ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta
    19801 tctttcagaa tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat
    19861 cttgagctcg ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca
    19921 attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc
    19981 tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc
    20041 aggattggcc ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc
    20101 gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc
    20161 tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc
    20221 attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat
    20281 ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc
    20341 tgccgtgttg attattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg
    20401 attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg
    20461 aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat
    20521 ctacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa
    20581 aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg
    20641 ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa
    20701 ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg
    20761 gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg
    20821 aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa
    20881 caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct
    20941 ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg
    21001 ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca
    21061 cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca
    21121 gctcgtatca gcgactatac tgcctttttc tatttaggaa aagtaatgga atatgacaaa
    21181 actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat
    21241 tttggttaag acggaggcct caacatgaca aaagagatta tttcatctaa agatcttcat
    21301 ctgtattatg gaaagaaaga agcactaaaa gggatcgatc taagcttcaa tcaaggggaa
    21361 atcactgcga tgatcggtcc ttctggttgc ggaaaatcca cttatctgcg ttctttgaac
    21421 cggatgaatg acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa
    21481 gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc
    21541 cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta
    21601 aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg
    21661 tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg
    21721 cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg
    21781 gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta
    21841 gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt
    21901 atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa tgatacgaag
    21961 aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga
    22021 taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat
    22081 cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac
    22141 atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac
    22201 atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca
    22261 actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc
    22321 gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg
    22381 gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc
    22441 ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa
    22501 agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat
    22561 cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc
    22621 ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact
    22681 gagttaaata cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt
    22741 taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag
    22801 aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga
    22861 tgcaagcttt tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc
    22921 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata
    22981 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta
    23041 cctgtcatcg agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa
    23101 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
    23161 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
    23221 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
    23281 attggcgcca tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc
    23341 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct
    23401 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
    23461 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
    23521 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
    23581 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
    23641 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
    23701 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
    23761 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
    23821 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
    23881 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc tatccctatc
    23941 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
    24001 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
    24061 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
    24121 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
    24181 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
    24241 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg
    24301 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
    24361 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
    24421 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
    24481 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
    24541 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
    24601 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
    24661 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
    24721 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
    24781 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
    24841 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
    24901 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
    24961 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
    25021 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
    25081 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
    25141 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
    25201 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
    25261 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
    25321 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
    25381 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
    25441 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
    25501 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
    25561 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
    25621 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
    25681 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
    25741 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
    25801 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
    25861 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
    25921 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
    25981 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
    26041 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
    26101 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
    26161 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
    26221 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
    26281 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
    26341 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
    26401 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
    26461 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt
    26521 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
    26581 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
    26641 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
    26701 gaggtgagac atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg
    26761 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
    26821 tgtattcctg agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt
    26881 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
    26941 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
    27001 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
    27061 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
    27121 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
    27181 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
    27241 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
    27301 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
    27361 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
    27421 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
    27481 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
    27541 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
    27601 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
    27661 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
    27721 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
    27781 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga
    27841 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
    27901 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
    27961 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
    28021 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga
    28081 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
    28141 cctggaacat ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
    28201 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
    28261 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
    28321 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
    28381 agtagccgcg atgaatttcc gcgcacgtac aatgggcttt gacgcaacga aaacatttga
    28441 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
    28501 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
    28561 gcattgatca tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt
    28621 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
    28681 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
    28741 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
    28801 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
    28861 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
    28921 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
    28981 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
    29041 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
    29101 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
    29161 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
    29221 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
    29281 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
    29341 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
    29401 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
    29461 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
    29521 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
    29581 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
    29641 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
    29701 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
    29761 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga
    29821 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
    29881 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
    29941 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
    30001 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
    30061 agcaatgggt gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt
    30121 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
    30181 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
    30241 agcagcgatg gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta
    30301 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
    30361 tggaaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
    30421 gttaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
    30481 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
    30541 gaagcgttga aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
    30601 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
    30661 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
    30721 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
    30781 ccatttgtcg ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa
    30841 ttaatcgatg attacgaacg tactcatgct tctacgatcg ccgttatgca agttcctcat
    30901 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
    30961 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
    31021 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
    31081 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
    31141 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
    31201 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtacatc
    31261 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
    31321 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
    31381 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
    31441 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
    31501 attgatcgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
    31561 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
    31621 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttac tggtcaaaag
    31681 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
    31741 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
    31801 gaaagtcggc ggaatcggta aaacgacagc taaagcgacg gttgccggaa aagtcggtgg
    31861 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
    31921 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggcacagc cgcagcaatc
    31981 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
    32041 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
    32101 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
    32161 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
    32221 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
    32281 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
    32341 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
    32401 atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa tgatgaaaat ggcaaaagaa
    32461 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
    32521 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
    32581 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
    32641 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
    32701 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
    32761 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
    32821 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
    32881 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
    32941 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    33001 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgta tttcctggtg
    33061 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    33121 aaacaccttg ttttttcaat tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc
    33181 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    33241 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    33301 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    33361 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    33421 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    33481 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    33541 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    33601 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    33661 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    33721 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    33781 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    33841 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    33901 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    33961 gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag caacaagaaa aaaagtcttg
    34021 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    34081 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    34141 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    34201 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    34261 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    34321 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    34381 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    34441 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    34501 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    34561 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    34621 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    34681 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    34741 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    34801 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    34861 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    34921 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    34981 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    35041 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    35101 agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc tgcattttgt
    35161 tcaccaccat caaaaaaata agggactgtg acaatactat tgtcacaatc ccttattact
    35221 gtgtattcta ttaaatcaat catctttctc atctgattct ttagttgctt gattcgctct
    35281 tgcataagct gaagcggtgt tccagtaatc tgtatagttt tcatcggtac cgccaacgcc
    35341 aaatttaaag gcgcttttct tcggtccatt tttcgcccat agcgattcaa ctgtttttcc
    35401 tggtgtttgg atggattttt tattgacagt aacttttcca ggtgtttgac cagtaaatcc
    35461 agatactttt ttcttcatca catcagatga taattcgaat ttctcatcag tgccaaaaat
    35521 gtcaggtttt gcttggtaag cttgattgat cagattggca agataattag ccgaacgtat
    35581 ccttgctttg ctgtccattc cagtattgtc atcgtgccct gcccagctac tgatggtaat
    35641 agacggagta gagacaatta gccatgaatc tcggtattca ttggtagaac cagtttttcc
    35701 gacccaatct gcttttccta gatttccgtt taagcttgag atggcatctt tgaatggtgt
    35761 agtgattttt gcattgatca cactacgcat catgtcattc atgatcgatg ccgttgcttc
    35821 cgaatagatc cggacagagt tgctttcgtg tttataaatt acatttcctg cgttatctgt
    35881 gatggaatcg atgatatagc cttcttcata gacgccgccg tttgctaaag cctgaaatcc
    35941 gttggtctgt tgaagcgtcg tgacatctac tgtacctaaa ggagcagatt caacgcccca
    36001 attgtcattt gccgggtagt tcatctttgc caagtagtgc tcgtaagcat attgtttcga
    36061 tccaccggaa ttcaacacat cttgatataa gtgataagcc gggatattgt tggaccattc
    36121 tagtgactca cggacggttt ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt
    36181 ctcgccagca ttttctcctt cttgccaagt tgttgcataa tctgatacac gggattctga
    36241 tccgataagc cccatgtcga ttgcaggtcc ataaacaaga actggtttga ttgatgagcc
    36301 agcttgacgt tctgtatcga aggcatggtt attttgattc tgactgtaat tccgtccgcc
    36361 aacgaatccg tagatgcggc cagttctgtt atccatcaat acatttccgg tttctacttg
    36421 actgccagca ccatcatcta acatgtatcc ataattttca acgccatttt gcaatgcagc
    36481 atagacatct ttatcgattg tcgaatggat tgtatatcct ttattttgga tcgtttgctg
    36541 tgccttttga taataagcgt cactgaccga tgaatcagac atatcagcgt tgtctttttc
    36601 agccaattgt tgtgcaatga ttcttgttgc ttcattcatc acagtataat acaagaattc
    36661 tctgtcagtc tgttctgcga tttgttgtgg tagaaagtct tttgtaaggt cataggcttt
    36721 tgcttcttcg tattgttctt tcgtaatttg gccttcacga tacatactga ataaaacaaa
    36781 atcttttctg gcaaggccgg cagaaagatc ttcttttagc gcgcctgtgt tcgtgtatgg
    36841 actatacgtg atcgggcttt ggggtaatcc agcgatataa gcagcttgcg gcagcgtcac
    36901 atcttttgca tctacaccaa agattccttt tgcagcttcc tggactccag caatgttttg
    36961 tcccttatta ttacgaccaa atggtgagac attcaaatac gtagcaatga tctcatcttt
    37021 tgaaaagtat ttttctactt gtgcagaaag aatgatctcg tttgctttac gtttgaatgt
    37081 tgtttcatct gtaaggatct gctgtttgac taattgctgt gtcaaagttg atccaccaga
    37141 agagccaata ccagtagctt cagatactaa tgctcgtaaa acagctttag gaacgtaacc
    37201 ttggtgttta tcgaagtatt catcctctgt gctgataatt gcagttttca ataaaggtga
    37261 aatcttatca gatgaaatag tcgtgcgcat cagatctgtc tggatttttg atatctctgt
    37321 attatctgca taaacaatct ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc
    37381 ttttttagtc ggcaattgtg tgtcttcgac gagataggca aaatagccag cgcctattcc
    37441 aatacctaga gctgcgaaca tcaaaagaag aacagtaata aaaacagtca atgattgaaa
    37501 aactctaaaa ataatcttag gaacaaacca tttttctttt tttcttctat tctgtttttt
    37561 tcgagtattt ttgcgtggca aaagggtcac ctcaactaat ggttttattt cgctaattat
    37621 agcaaataat agaacaaaaa aacagacaag gctgtttttg gcaggaattt cttttttaaa
    37681 agagttaaaa cgtgagaaaa tgatctttat tataaaagag ttcttaagct tttctttaga
    37741 gaaaatttca gttactatgg agaacagggc aaagaaaaat caatcatttt ctttgtatat
    37801 catcttacca atatatagga attgttagta acaattgcct ttcaaggagg aatccatcca
    37861 tgttattagg ctcacacgtc agcatgagcg gaaagaaaat gctgttagga gcagcagaag
    37921 aagcagccag ttatggatct tcgactttta tgatctatac aggcgcccct caaaacacta
    37981 gaagaaaagc catcgaagag atgaatattg atgccggaaa agcgtacatg gcagaaaaag
    38041 gtctaacaaa tatcgtcgtt catgcccctt atatcattaa tttggggaat acgatcaaac
    38101 cagaaatgtt tccttttgcc gtccaatttc tgagagaaga gattgttcgt gcggaagctt
    38161 taggcgctac tcaaattaca cttcacccag gtgctcatgt aggggcagga acagaagccg
    38221 gaattgctag aattattgaa ggcttgaatg aagttttgac aaaagaacag acggcacaaa
    38281 tcgctttgga gacaatggca ggtaaaggta cggaaatcgg tcgaacattt gaggaattag
    38341 cgcaaatcat cgatggagta aaattgaatg aaaaattatc cgttactttt gacacctgtc
    38401 atacgaatga tgccggttat aatattaaag aagacttttc aggtgtaatg gaagagtttg
    38461 ataagatcat cgggttagat cggcttaaag tactccatat caatgattct aaaaatccgc
    38521 aaggttcgca taaagaccgg cacgccaata ttggttttgg aacaatcgga ttcgacacgc
    38581 tgaataagat cgtccatgat ccaatgtttg acaatatttc gaaaatcttg gaaactccct
    38641 atgtaggcaa agataaaaag aaagcttacg caccatatgg aaaagaaatc gccatgttca
    38701 aagcgcaaac attcgatcca gaagtgtttc ctgagttatt agcagaagaa gtgatactct
    38761 aagattttct atctgacagc agcaaaaaag gaccaggcac attgccttgt ccttttttgt
    38821 ttaagaaaca aaggagtact ttttgcagtc tttttcatca tgcatcttct cccccctctt
    38881 tttaaattta gacaaacaag gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa
    38941 gcaagtggca aaacgtgatt ggaaggacga aaagatgctg aacactttaa aacccttgat
    39001 caaccaacta aaaaagaaac atacatactt tattcttggc atagccctat tatttacaat
    39061 tattcgtttg tatttatata gtaaagcaat ctatggaaca gacatgtcgg caacctatga
    39121 tgaccagctg ctgattcgct atgctcaaaa tattttggac ggtaaatggc taggggagta
    39181 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
    39241 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
    39301 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
    39361 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
    39421 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
    39481 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
    39541 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
    39601 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
    39661 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
    39721 tcctatagtt ggcttttcca tcgtctatca atggatcgaa tataaaaatg agacaacata
    39781 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
    39841 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
    39901 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
    39961 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
    40021 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
    40081 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
    40141 caagggaaca atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
    40201 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
    40261 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
    40321 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
    40381 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
    40441 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
    40501 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
    40561 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
    40621 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
    40681 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
    40741 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
    40801 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
    40861 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
    40921 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
    40981 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
    41041 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
    41101 gctgttggtc atcatcagta aaggaatcat cgaaaacggc aacgcaaaag ccaagaacac
    41161 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
    41221 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
    41281 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
    41341 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
    41401 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
    41461 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
    41521 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
    41581 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
    41641 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
    41701 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
    41761 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
    41821 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
    41881 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
    41941 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
    42001 aatatctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ctaatgtctt
    42061 acttgtgcgt gctcggattg cctgtgccaa atccatttgg ctcacgattc ctaattttgc
    42121 tgccatatac tgtaaaagca tagcaatcag actagacatc aaaataactg acatcaataa
    42181 atattggaaa ttttgtcctc cggtgatcga agtagaccag ttacccgggt ccatataccc
    42241 aactgctact aacgcaccag gtccagagta agcaagaagt gttttaaaaa aacttttccc
    42301 ctttggtaca tcaactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
    42361 aagtcgatgc ttatgaaatt gtttattgga ttgattattc aagaaaaaac cacctttcct
    42421 atttatctac gattacattc tacacctcaa aaagaaaaaa ttaaagaaaa agattcggta
    42481 cgcctaattt tttttcatgg cgcgtcttgc tagatgacga aagaaatttt gttctcctct
    42541 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
    42601 ttagtaaaat aagtcattga gagcaattcg gaaatagaaa ggagagattt atatgttatc
    42661 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
    42721 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
    42781 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
    42841 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaac gactttttac
    42901 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agattattca
    42961 gcaaaaaaga caagcacaga caatcctagg ctcttgataa agacaaagga ggagtaaaat
    43021 gtcgattttt agaaaattag gctggttttt tcgtcaggaa aagaaacatt atttaattgg
    43081 ggttttctcg ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
    43141 catcattgat gagatcgccg ataaaaattt atcaataaag atcattttcg ggtggatctt
    43201 cctattgatt atcgctgctt ttttacaata tctttttcgt tatatctggc ggacgaatat
    43261 ttggggaagt gcagctcgat tggagaaaga acttcggcag cagttattca aacattttac
    43321 agaaatggac aatctctttt accaaaaata tcggactggg gatttgatgg ctcacgcaac
    43381 gaatgatttg aatgcgatcc agaatgtagc aggagctggt atcttgacct ttgcagattc
    43441 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
    43501 actgattgcg ctgattcctc tgcctttgct ggccgtcact tcacgagtgc tggggtcaaa
    43561 actccatgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
    43621 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctagc
    43681 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
    43741 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
    43801 tttaggtgga aagtatgtct tggaaggcac gatctcgatc gggcagatgg tctcatttat
    43861 tagttatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
    43921 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
    43981 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
    44041 ctcttttaat tatccgaaag aagaatcacc agcattagaa caaatcaagt ttgcgatcca
    44101 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa
    44161 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
    44221 caaagaatat tcattgaatg ctttattagg atcaatcggc tatgtgcctc aggatcactt
    44281 cttgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
    44341 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattaccaaa
    44401 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacaaag
    44461 gatttcgatt gctcgagcat tgatcaaaaa tcctgaatta ttaatattag atgatgcact
    44521 ctctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    44581 agagaaaaca actatcattg cagctcaccg tttaagtagc gtgatgcatg caaaagaaat
    44641 cattgttgtc gatcaaggga aaatcattga acgaggaacc catcatgagc tgcttgcact
    44701 aggcggctgg tatgaacaaa tgtgggagaa acagcaatta gaagcgaaga tagaaggagg
    44761 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    44821 cagaaaaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    44881 gttgctatct tatttgcgtt tgctttgtct gtgatcaatg tcctattgcc aaggatcatc
    44941 caaactttta tggatgatca tctggctaaa cagtcggcta cgacacaagt tattttgttt
    45001 tttgctggta tgtacttatt tggagtcata gtaaaaagta tcatttggtt tttccaatgg
    45061 tatttgtatt ccatggcctc attgaagacc tatcagtata ttagggtaaa attatttgaa
    45121 aagctgcata cactaggtat gcgttatttt gatcagacac cggcaggatc aaccgtttct
    45181 cgagtgacga atgacacaga aacgctattt gaattttggt acgtcttttt gatggtattg
    45241 actgggatct ttgcagtcgt ttcttcattc cttgccatgt ttcaaatcaa tggagaaatt
    45301 gccttgtgga ctttggtgtt tttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    45361 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    45421 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    45481 aaagaatttg aacaagtgaa caatgagtat ttagacacgc gttatgcgat gatcaaaacg
    45541 aattcgattc tgttaagtcc gatcatcaat tttctatatg cattggcgat tgcgttggtg
    45601 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    45661 tttacgacct atgtccaggg attttttaat ccaatgtctc aaatgatgga ctttttgagc
    45721 atcttcacag atgggatggt tgctggtagt cgtatcttga agattatgga tacggaagaa
    45781 tatgcaccac aacaaaaccc tggagcagat caaatgatta cttcaggtaa gattgagttt
    45841 cgcaatgtaa gcttttctta tgatggagag cacaatgtat taaacaatat ctcttttgtt
    45901 gctaatccgg gagagaccgt tgcattagtg ggacacaccg gaagcggaaa aagctcgatc
    45961 atcaacgtat tgatgcggtt ttacgaattt tatgaaggtc agatattgat tgatgatcat
    46021 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tggggctggt cttgcaagat
    46081 gctttcttgt tttatgggga tatcgcagga aatattcgga tgatgaatcc agctatcaca
    46141 gacgaacaga tacgccatgc ggctgagttt gttcaagcaa acaaattcat cgatgaatta
    46201 ccaaaaggat atcactcaaa agtgatcgaa catggtgcca gctattctag tggacaacga
    46261 cagctgatca cttttgcgcg aacaatcgtg actgatccta aaattctagt attggatgaa
    46321 gcaacagcaa atatcgatac tgaaacagaa ggattgatcc aagaaggatt ggcaaaaatg
    46381 cgtcaaggac ggacaacgat tgcgattgcc catcggctgt caacgattcg agatgcagaa
    46441 ttgattttag ttttagacaa aggtcatata gtagaacgcg ggaatcatga aacacttcta
    46501 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtat ataaaagatg
    46561 ttgtgacagt agaattttga cctaagcact agagtgaaaa taaaacatag cttctcatat
    46621 ttttcatctg tgagagttag tgtcgtagtc accagattta aaaaaagatt cgacagctag
    46681 tcagaagtgc ctaatagcca ctaagaaagc gttgacaaaa gcatttgctt tcgataatat
    46741 aaagaaaacg atacaacttg gatagtgatt gcctgatgca agctaaacct gatgagtctc
    46801 ccatggtttc atggacggct ttttgggttt ttctttttgt tttggataca gccctataca
    46861 tcatggaaag gagcaaaaca tgctgattga gaaagtactg aataacaacg tcgtaataac
    46921 cagggatgaa agaaatcagg aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa
    46981 agtgggtgac ccgatttttg actcatgcat cgacaaaaaa tttcagttga caaatgacgg
    47041 gctttcacaa cagttccaag aattggcaca agcgattcct cttgagtact tagaaatttc
    47101 ttgtgaaatc attgaatata caaaagaggc tctacaagtc cagttgaatg attctgtgta
    47161 tattgcattg acggatcatt tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt
    47221 accaaatgta ttgctgtatg atatcaaaca actatttcct aaagagtatg ccatcgggaa
    47281 aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag atgaagccgg
    47341 atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccagcaag acaataccta
    47401 cctgtttact ataacaatac aggatattct aaatatcgtc cgctattact ataagattac
    47461 atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt tttttctgac
    47521 acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat tgtttcaggt
    47581 tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag actttttagc
    47641 cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga tccatactgc
    47701 gcgtttagtg catgagaatc gttgtaaata aaaaaaggag gataaattaa caagaacgta
    47761 tgaaataagg gtagtgacat tcagttggct aaaccttcca ctaataaatt ggagggaata
    47821 gaaatgggaa aataccaaga attagcgaga aaaatcgtag aaaatgtcgg cgggaaggaa
    47881 aatatcaatg gtttgacaca ttgtatcaca aggcttcgtt tcaaattaaa aaacgaagaa
    47941 aaagcaaatg atgagatctt gaaaaatatg gacggtattg taacggtaat gcgagcggga
    48001 gggcagtatc aagttgtcat tgggaatcat gtgccagtcg tgtttgaaga ggttataaaa
    48061 gcaggaaacc ttacttttga tgaggctgtt tcaacagaaa aaatgagacc gtttgatatg
    48121 cttattgatg gaatatcggg ctgtttccag ccattccttg ctatcctagc tgctggcggg
    48181 atgattcgtg gactgacagc attccttgtc ttcttaggcg cgtttgaccg cggttctggc
    48241 acatttgtta tgtttgataa tatcggtgat tctgtattcc aatttatgcc tgttattatc
    48301 ggtttaacag ctgcccgtaa atttaaagta aatgaattcg tagggatgtt gcttggcgca
    48361 gcattaatga atcctagctt gtcgctggaa gctttgagtg gtgcagcaga agcaccacta
    48421 acgaccatat tttctggcac tatttttgaa gcacctattt atcaaacagt attcggcatt
    48481 ccctggattg cgcgtaacta cgcttcttct gtcattccca ttatctttat tgtgttgtta
    48541 gctagccaaa ttcagaaacc aattaaaaag ttagtacctg agatgatcgc gaatttcttc
    48601 gttccattct tcaccgttct tattacaatg cctttaggtt ttttactcgt tggaccagta
    48661 ttcacattcg caactgatat tttaatggct ggatttgaaa cacttttagc actttcgcca
    48721 gttatttatg gagcaatcgt aggcttcttc tggcaaattc tggtgatgtt tggtctccat
    48781 tgggcaatcg taccaatggg attgatgcaa ttttctgtga atggttggca aaatattatg
    48841 acacctgttg cagtagtgtc ttttggacaa actgcagcac ttaccgcact ctactttaag
    48901 cttagaaatc caaaagacaa agcaattgca ataccagcca ttgtttctgg tattgtgggg
    48961 attacagagc cagctattta tgggtttaca ttaccacgta aaaaaatatt tatttttacc
    49021 tgtgtcggtg gtgcgttagg gggcgcgtat agtgggttaa tgaatttaac ttcttggaat
    49081 caaggtggac taggcatctt tacaattccc aactatatta gaccagatgg tgatttaaca
    49141 gatgtgatta atgtattgat tgggatcgcc attgcgatgg tggtatcctt tacgctgact
    49201 tttttctttt ggaaggatga agcaggtgaa acagatgata tacagaaaaa atcaggtaaa
    49261 gaaattgtaa aaacaccgat acaaggacaa atcgctccac taaatgcagc aaaggatgca
    49321 gcttttgcac aaggaacact aggtcgagga atcttgatct accctgaaaa aggagaagta
    49381 cgtgcccctt ttgacggaac agtcatgacc ctgttcccga cgaaacaggc aattggaatg
    49441 gtttctgaaa cagggctgga attgctgatt catgtcggat tggatactgt gcagttggaa
    49501 gggaaatatt ttgaaagctt agtgcagcaa ggataaagag tgaaacaagg cgatctgttg
    49561 ctccgcttcg acaaaaagaa aatcgaaaag gaaggataca gtttagaaac acctgtaatc
    49621 gtcaccaatt acatggatta tttggatatt ttggaaaatc aaggggagac ggtcgggcca
    49681 gacgatgcac ttatcaccgc actgacctga acggttaggt gaaagaatgg aatagactta
    49741 actattcttt ttagtccact tctttatttt caaaattgaa aggctctttc atgatggctg
    49801 agctgatagg gcgtttatca aatttttgtc ctacagaaat tagatggact aaattctgtt
    49861 gataattttc tgcaaaaaca caactatata acacgtctag aagataacaa atcgatgtat
    49921 tgatcgtaaa attaccaatt tttgaataca gtcgttcgcg tgtcgtaagt cgcaaagcgg
    49981 ctgtactcat tgaggatagc gttgaatcac cgatactggt caacgctaaa attggaacat
    50041 gattttgttt caatatttct gcggtacgaa ggatcacatt gttttcaccc gtataagaaa
    50101 tcagaatccc gcacatatca ctcgtgcaat tgtacgcttc atagaacgat tcaccaaaag
    50161 tggtgacaat cgtgacatcc attttgatgc gcttcatttt cagcatgaaa tcttgagaaa
    50221 tcaaagtatt cgagttactg gcgaaaatca cgattttttt tgcttgaagc attagttgtt
    50281 tggctttttg cagttggtca tgatgaatga gagaaagtgt atcttgaatc gtcatctgtt
    50341 ctaaagaagc cattttattt gcgatcgtca taataccgtc tgtggtggaa aaaggaagat
    50401 tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt ttcagctaaa aaagcattac
    50461 gaaaactgac ccagccatca aaacccattt tttttgctac acggatgagt gtggacgggt
    50521 ggacataaca tcgctcagca atctcttgga tggtcatttc gccaagtgct ggagaatcgc
    50581 ttaagagaaa ctgaatcact tcattttctg aaggagaaaa ggagatttct ttgatttttt
    50641 cacgtaataa catgtagata ccttcttcta gtttttttac gtaaattata acttttttga
    50701 aaatgatttg taaacggatt tatgttatat agcttaataa agtcattttt tgacaagata
    50761 tttacaaaaa tccagtagaa aacactaact tttgtaaatg ttactggaaa gaaacgaatt
    50821 tctattgttt gttttgttta caaaaaagat aataaaagtg taaaaagcaa aggagtgaga
    50881 acatggcaaa aggaatcaaa atcgctacga ttggtggagg cagcagttac acccccgagt
    50941 tgatggaagg atttatcaaa agatatgagg aattaccgat acgagaaatc tggctcgtag
    51001 atgtagaaga aggaaaagaa aagcttgaga tcgtcagtca gatgtctcag cgtatgtggg
    51061 atgcttctcc gtatgaagtc aagatctatg caactttgga tcgggaagaa gcattgaaag
    51121 atgcggattt tgttacgact cagtttcgtg tcggtcaatt agatgcacga atcaaagacg
    51181 agcggatccc tagttattat gggatgattg ggcaagaaac aaatggtgct ggcgggatat
    51241 tcaaagcttt tcgtaccgtt ccagtggttt tggcaatcgt tgaagacatg aagcgtttat
    51301 gtccaaatgc ttggctgatc aatttcgcga acccaagcgg gatggtgaca gaagcagtta
    51361 tcagatatgg caaatgggat aaagtgatcg gattatgcaa tgtgccagta atggcgacga
    51421 tgacagaacc agcaatgctt ggcaagaaaa aagaagagct gatctacaag tttgccggtc
    51481 taaatcactt ccattggcac aaagtagcag ataataaagg aaatgaccgc acagcagatt
    51541 tgattgatcg tttatacgat gaagacaatg gattgccgaa aaatatcttt gacatcccgt
    51601 ttttcaaaga acaattaaaa caaatgaaca tgatcccttg tggctaccac cgctattact
    51661 accgtgaaga agaaatgttg gaacatgctt tagaagaata tcaaacgatt ggtacaagag
    51721 cgcaacaagt gaaagaaaca gaagcagagc tgtttgaatt atacaaagat ccagaattgg
    51781 atcataaacc tgaacaattg caaaaacgag gtggtgctta ttattcagat gctgcctgtg
    51841 aaacgattgc ttcgatctat ggaaataaag aaacacagat cgtggtgtct acaaagaatg
    51901 atggagctgt tcctgatctg ccggcagatt gtgtagtaga agtcactgcc tatgttggcg
    51961 cacaaggcgc acgaaatgta gcgtttggtt cccttcctgc tgctgaaaga ggctggttgc
    52021 aagtaatgaa gaatatggaa ttgctgacga tcgaagcagc cgtgactggt gattatggaa
    52081 cagctttaca agcgtttacg atcaatccgc ttatacgatc aggaaagaca gcaaaacgga
    52141 tcatggatga gttgtttatt gcacatcagc agtatttacc gcaattcaag gaaacaatca
    52201 gccggttaaa agcagaagga atcgaagtcc aagatgaact ggcaagagaa ttgtcaagca
    52261 atacacatgt aatggaggaa gtatgatggg attcagaaaa gattttttat ggggcggtgc
    52321 tacagcagcc aatcaatgcg agggtggtta tgatgaaggc ggacgtgggt tagcgaacgt
    52381 agatgttgtg ccaatcggag aagaccgttt tcctgtcatt actggaaaaa agaaaatgtt
    52441 tgaatttgat aaagatcatt tttatccagc aaaaattgcg atcgatatgt atcatcatta
    52501 taaggaagat attgctttgt ttgcagaaat ggggttcaag acttatcgga tgtccattgc
    52561 ttggagtcgt atttttccaa tgggcgatga aaatgagcca aatgaatctg gtttgaaatt
    52621 ttatgaggat gtctttaaag aatgtcaaaa gtacgggatc gagccgcttg tgacgattac
    52681 acattttgat tgtccgatgc atttgatcaa agaatatggt gcgtggcgtt ctagagaact
    52741 cgttggtttt tatgaaaatc tgtgccgagt catcttcaat cgctataaag gacttgtcaa
    52801 atattggttg acattcaatg agatcaatat gatccttcat gcaccattca tgggcgcagg
    52861 gctgtatttt gaagaaggag aaaatgaaga acaaatcaaa tatcaagcgg cacatcatga
    52921 gttggttgca agtgccatcg caacgaagat tgctcatgaa gtagatccgg agaacaaagt
    52981 aggctgtatg ctggcagcag gagcatatta tccatacact tgcaatcctg aagatgtttg
    53041 ggcttctaga aaatcagatc gagaaaatta tttcttcatt gatgtccagt cacgaggaga
    53101 atatcccgct tatgcattga aagaaatgga acgaaatggc attcagatca aaatggaacc
    53161 tggggatgcc gcattgctga aagaacatac tgttgatttt atctcctttt cttattattc
    53221 ttctcgagtt tcgactactg acaacgaact cttggaacag actgcaggaa atatctttgc
    53281 ttctgtcaaa aatccatatc tggaagcaag tgaatggggt tggcaaattg atccattagg
    53341 cttgagaata acgatgaatg atatttatga tcgttatcaa aaaccactat ttattgtaga
    53401 aaacggctta ggtgcagtcg atacgcccga tgagaatggt tatgtggaag atgactatcg
    53461 aatcgactat ctggcagcac atatccaggc aatgaaagat acagtagaat tagatggagt
    53521 cgatctatta ggctatacga cttggggatg tattgattta gtatcagctg gtacaggtga
    53581 gatgaagaaa aggtacggat tcatttatgt cgatcgtgac aacgaaggaa atggcacatt
    53641 agcccgttcg aagaagaaat cattcaactg gtataaaaaa gtaattgatt caaatggaga
    53701 aaatctttcg aatgattgat caatgatttg taaaagtcta ttgtttttca gcggattttt
    53761 cgagtataat aaagctattc gagaaacgca agcagaacaa tagacttttt ttcgcattgg
    53821 ctgtaaagta tgcttacttg attaagtaaa ggagaatcta catatgtatg atgtaatcgt
    53881 tattggcgca ggtcctgctg ggatgactgc tgctttatat gcttcacgtt caaacttatc
    53941 cgtattgatg atcgaacaag gcgcccctgg cggccaaatg aacaatacgg cagaaatcga
    54001 aaactatcct ggtttcgatt caatcatggg tcctgatcta gcttataaga tgtatgaagg
    54061 tgtttcaaga tttgggacag aaaatgctta tggaatcgtg caagatatca aggatcacgg
    54121 cagccacaaa gaagttattt gtgaagataa aacttacgaa ggaaaaacag tcattatcgc
    54181 aacaggctgt atccaccgta aattaggcgt acctggcgag gaagaatttg ctggacgtgg
    54241 cgtttcttac tgtgcagttt gtgatggtgc gtttttccgt aacaaacgtc tagtagtgat
    54301 cggaggagga gactcagcag tagaagaagc catctatttg acacaatttg cttctgaagt
    54361 agtcatcgtc caccgtagag atgaattacg tgcacaaaag atcatccaag accgtgcatt
    54421 tgcgaatgaa aaaatttcct ttatgtggga tacagtcgtt gacgaaatcg ttgggaatga
    54481 aatggtagtg acaggtgtga aagcacgtaa tgtcaaaacc ggcaatgagt ttgatttggc
    54541 agcagacgga gtattcatct atgttggact ggatccatta actgcaccat ttagagaagc
    54601 agggttgacg aacgaagctg gctggattcc aacagacaac gaaatgaaga cgaatatacc
    54661 aggcgttttt gccatcggag atgttcgtga aaaagatttg cgacaaatca ctactgctgt
    54721 tggtgatggt gggatcgctg gacaacaagt gtataaatac ttggaatctt tagaagaaac
    54781 aactgcataa gtttaaaaag agtggaagaa cggtttcaat tgttctttta ctcttttttt
    54841 tcgcgtgaaa tctactgaat ccgatttatg cgaatacttg caaccgtgct tgataaaaga
    54901 atcctttctt gatttaagcg ttttttagac ctcgtacttt catatctaat aagtgaatgc
    54961 tatacttatt aagaaccata tttcagaaag aggtgttatt ttcatgtctt gggaacaagt
    55021 gtatgaacaa tgggtacatg aagaaaattt agaagaaaac ttaaaaaaac aattaattga
    55081 tctgggtgaa gatcctgaaa aacttgaaga tgctttttat gcaccgttgg aattcggtac
    55141 tgcaggcatg cggggaatct taggacctgg gatcaatcga atgaacatct atacggtaag
    55201 acaagcaaca gaaggacttg cacgtttcat ggatacacaa ggtgaagaaa caaaacgccg
    55261 aggtgtcgct atcgcttatg actcacgcca tcaatcacca gaatttgcca tggaagcagc
    55321 caaaacatta gcacatcaca atatcccttc ttatgtattc gaaagtttac gtccaacacc
    55381 agagctttct tttgcagtcc gtcatttgaa gacgtttgcg ggaatcatgg taactgcatc
    55441 ccacaatcca gcagcttaca acggatataa agtctacgga gaagatgggg gacaaatgcc
    55501 tcctgcagac gcagatgcat tgacgaaata tgtccgtgaa gtcagcaatc cgttaaaagt
    55561 tgaagtatta tctgacgacg aagctgaaca tagcggattg atcacgatca tcggagaaga
    55621 agtggatgcc gcttatctag aagaaatcaa agcagtaacg attgatcatg aattggtgga
    55681 aacaatgggt aaagatttga aacttgtgta cacaccactt cacggaacag gaaaaatgtt
    55741 gggcgaacgt gcgttgaaac aagctggatt cgaacaattc gtactagttc cagaacaagc
    55801 tgtcgcagac ccggatttca ctacagtcaa atcaccgaat ccagaagaac actctgcttt
    55861 cgagtatgcg atgcgacttg gcgagaaaga aggcgctgac ttactgattg caacagatcc
    55921 tgatgcagac cgtttaggcg cagccgttcg tttgccaaat ggctcctatg aagtattgac
    55981 agggaaccaa atcggagccc tgtttgctca atatattttg gaagcacacc aatcgcgagg
    56041 cactttacct gaaaatgcag cggtattgaa atcaatcgtt tctagcgaat tgcctacagc
    56101 aattgcggaa cactatgggg ctaccatgtt caatgtattg acaggattca aattcatcgc
    56161 tgaaaaaatt caacaatttg aagaagatca ttcccatact tttatgttcg gattcgaaga
    56221 aagttacggt tatttagtta aacctttcgt tcgcgacaaa gacgcgattc aagcattgct
    56281 cttattagca gaagtagcag cttactataa aaaacaaggc aaaacgttgt atgacggctt
    56341 gcaaacgatt tttgaaacat atggctacta cgcagaaaaa acgatttctg tgacaatgag
    56401 cggacaagaa ggttctgcaa aaatctctgc tttgatggaa aaattccgtg aagaagcacc
    56461 gacagaattt gccggagcaa aagtcatcca aacagaagac ttcaaagagt tgacgagaac
    56521 agaccgtgaa ggaaacgtag aaaaactaac gacaccgcca tctgacgtat tgaagtatac
    56581 gctggaagaa aatggctgga tcgctatccg tccttcagga actgagccga aaatcaaatt
    56641 ttatatcggt gtaaaaggaa actcttcaga agacgcagct gaaaaagtag ccgcttatga
    56701 agcgattatc aaaaaaatga cagaagaata aaaaaacttg agactgatgg aaaaagtggt
    56761 aaactttaga agtcagtctt ttttcaagtt ggaggtgaat ggattgccag acacgcaaaa
    56821 aacaaaagta gagaaagtca gccaactatt caaaatgttg agtgacccca ctcgtctcaa
    56881 aattctgctg tatctgaaag acggggaaca aaatgtgaca gcaatcaccc aagcagtaga
    56941 aatggaacaa tctgctgtat cgcatcaact gcgtttatta agagaaaacc atgtcgtcaa
    57001 gtcccatcgt gagggaaaag cgattttgta tagtcttgat gattctcacg tactggatat
    57061 tttgaaccag acgctcaaac atgtggaaca tcaataacga taggtgcaaa tagagacaaa
    57121 ctggactgta tcaacgaatc ttcttcgctg acacagtcca gtttttttaa gaaaaagaca
    57181 agaaaaactg ttagtctgtt tcttcggtgg tttcagttcc ataataacta tgatcttcta
    57241 ggtctaaagc attcaaaata agtgaagctg tttcttcgat agataaagaa gcgacattga
    57301 ttactacgca gcccaatttt tcataaagat cattcgcaaa atcgagttca gctcgtattt
    57361 tttcgatatc ggaataagca gtatcaggat ttaatccgta agagcgcatc cgttctttac
    57421 ggatattgtt taaaatatct gggttattcg tcagtcccac gattttttta ggatcgatct
    57481 cccaaagctg tttgggaata tgtgcttgtg ggatcaaagg tagattggca acctttaaat
    57541 ttttgttagc taaaaacaag ctcaaaggcg ttttggaagt tctggatacg cctagcaaaa
    57601 cgacatctgc ttctagaaac ccacgaggat ctttcccgtc atcgtatttt acagcaaatt
    57661 ccattgcttt gatccgtttg aaatagttct cattcaataa atgaagggcg ccaggttgcc
    57721 gaatgggctt gataccagtt aagcgctcga tttctgatat cggcggagtc atcacatcaa
    57781 aactaaataa ttgatgttct tcacaaaaag aacgcgtcat ctcgatcaat tcttgattga
    57841 tcaatgtgtg caggacaagg gctttttctt ttagtgcatc ttgcagtgcc tgcatcaatg
    57901 ttttctcatc tgtcacaaat gttctgcgga ataaattgaa ctcaacggta ggatattggg
    57961 ccatagtggc ttgcgctaat tttgtcgcag tttctccagc agagtcagag atgacaaaaa
    58021 aagtaacgat ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat
    58081 gtttattata ccatactcta gaatgtgtac ttcgtaatca ttttgatttg gaggaataag
    58141 tcatgaaaaa acagacattg attgaacaat cactagaaac attgaaaaat aacggtttta
    58201 aatatactaa aaaaagagaa gcattgttaa cttatttagt gaagcgcaat cgctacgtat
    58261 ccgctaaaga agtttttgac tatatgaatc aggaattcaa aggagtaagt tacgatacga
    58321 tttatcgaaa tctccatgat tttgcagaaa tcgatttact ggaagaaacc gatttaaatg
    58381 gagagatgaa gtttcgtttt cattgctgtc aaggggaaat cggccatcac catcacttta
    58441 tctgtacggt gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag
    58501 aacagctcgg tggatgtacg atcgaagggc atagattcga gattttcgga agatgtgaaa
    58561 attgtcaata aggatttaaa taaaataaca tttgataatt ttaaaaaaaa gcaagatttc
    58621 cttgacggtc aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata
    58681 tatttcaata acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa
    58741 aacagtcgtt cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt
    58801 gtcaaaagcg ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt
    58861 aagacgcaag aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga
    58921 attggagttg gtagtgtgtc actactaagt agattaaacg atgacatgaa aacagcgatg
    58981 aaagcgaaag ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac
    59041 gagcagatta aagaaggtca cgacttgaca gaagaagaag aattaacggt gttgtctcgc
    59101 gaaatgaaac agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta
    59161 gctgaaaaag taaaaagtga aattgtgata gtcgaaaaat acatgccgga acaactttcc
    59221 gacgaagaaa ttcgacaact tgttcaagaa gcaatcacac aaactggtgc ttcatcaatg
    59281 aaagaatttg gcaaagtcat gggtgcgatc atgcctaaag tcaaaggaaa agcagatgga
    59341 aaccaagtaa atgccattgt gaaagaacta ttacaatctt aattttatca tttaaactgc
    59401 ggacaatcat tgtccgcagt ttttttgtgc gcgtaaaaaa gagaacaaaa aaatagctag
    59461 acgaaagaat aggattcttt catctagctg gttttttcct gtttaatatt tcttatgtta
    59521 aattgtgttt gatttggtta ttcttctggt agttcatttg cttcttgttt ataccctgat
    59581 aggcgatagg cgtaataaaa cagtaggctg ccagcgtaac caagtaacgt tgggaccatc
    59641 cagccaagtc ctaatgcagc aaaaggaacg tgatgatgat aaaattcgag catgcttgct
    59701 acaactcttt gttgactcac taaagcaggc gaatttgcca gcatatccaa tacggcagga
    59761 acagcagcaa acaacatcgt tacttgatag acaaatggtg tcttttggaa aaattttgct
    59821 gttaaggaaa gcaagatcaa cgcaagcgac aatggataca aaagcatcaa tactggtaaa
    59881 gaccattgga tgatattgtc taaaccagca tttgcaacga caaaagagac gaaagtcgtt
    59941 aaccgcagcc atgtcatata actgacttta gggaacagtt tatggaaatc ttgcgcaaaa
    60001 gaaacaacga gtcccatagc ggtagtgaag acacctaatg tgaccatcac tcctaagaag
    60061 ctagcgccga agttacctaa ataaaaatta gtgatctgag aaagtgcagt tccgccattt
    60121 tctgataaag acatcttatt caaactcatc gcaccgagaa gaacaagacc aaaatagatc
    60181 atgacttcta gcgcaacgct caggctacca gcttttgctg ttaattctgt gactttttta
    60241 tctttgaaac caaggtcacg gactgcatgg acaaaagtga cacttaaggc aagcaaggct
    60301 actgcatcaa ccgtgttata accttctagg acaccatttg ttaaagcgtt tgtttggtaa
    60361 gtatctgcag cagattgatt tagatttccc attgggtgga ataaagaaac gacaaaaatg
    60421 attgctaaca atgcaagaaa caaggagttt agatactttc cgacgtattt gaccaaccgt
    60481 gtgggatgga cagtaaggaa ataagcaagt aaaaagaaaa gagcagagaa taaaaacata
    60541 cctaatgaat tatatttttc aggcaaaaat ggtttagcag ccatttcaaa agcagtagca
    60601 gctgtccgtg gtgtaccgaa aaatggtccg atcgtcaaat gaacaaggat taagaacaag
    60661 gcagcatacc attttccgac aggtttagct aagtcataca gaccatcact ttttgtcact
    60721 actacagcta agatagctag taatgggaaa agagcgcccg agatggcaaa tcctacgcca
    60781 gcactgaccc agtttgcgcc agccatttga cctaagtgga caggaaagat tagattacct
    60841 gacccgaaga acatcccgaa gataagtgaa ctgaccatca atagttgttt ccatgaataa
    60901 gtttttgttt cttccataaa tttttcctcc aataattatc attttgattg acgatataaa
    60961 cgttgcagca attagtaatg ccactaatat cagattccat gaaaaccacc accttaaaat
    61021 ataaaaaaag cccttttcca cttgggaaaa gggctctaga ttctagaacg gtaccacctt
    61081 ttttcgctta aaaaagcttc ttcattcacg gacgcttttt gataaagtta tccgctagcc
    61141 tgataatgga ggcagccaca atgatctacg ttttcgacat tgactcggaa atgatgttcg
    61201 tctattcgat ttattgtcat ttctcactta catgactcac tgggaataaa tcgttgaata
    61261 gcctactgtt ttcctcgacg ctttgctgta tgtttatttg ttgacgtaat aatacacctg
    61321 ttttttttga aatgcaagaa tattttaact attttgtttt ctttttttga aacaagaaaa
    61381 atacctagag aatacctgct gaatgaaacg gaatattttg ttctccactt tcatataact
    61441 tacattctca gcttcttttt atcaaaacga gacctttagc gaatggaaac ggctattttt
    61501 taacgtttct tggtacgtat ggttaaacga ctcttttaca accttttctt atttgctata
    61561 tagtttcttc tgagtatggt aaaataggaa agaatgaatc gaaaaaaagg ggatgtctat
    61621 ttttgacgga agagtctagt tctttagaga ttagattgac agctgctgat gatgtcagca
    61681 tgcttttagg tgcacacgac aagcatataa aactgatcga ggaaacaacg gaaacaacca
    61741 tccatacacg tggagaaatc attcagatca ttggtgagca atcccagatt tccttggctg
    61801 cctcagtcat tcggacatta caggaattga tcaaacgagg aatccacgta agtacgccag
    61861 atgttgtgac ggcattgaag atggggcaaa aaggtacatt agattatttt gttgagatgt
    61921 atgaggaaga gatcgtcaaa gaccgtaatg gaaaacctat acgggtaaaa aacagcggtc
    61981 aaaagaaata cgttgaatcg gtagccaaac atgacatcgt atttggtgta gggccagccg
    62041 gaacagggaa aacgtttctt gccgtagtat tagcaattgc tgccctaaaa aaaggcgaag
    62101 tgcaaaaaat cattttgacg cggcccgctg tggaggcagg ggaaaaccta ggctttctac
    62161 ccggggatct gaaagaaaaa gtggatcctt atctgcggcc tgtttatgat gctttatacc
    62221 aaatttttgg attggatcat acgaaccgat tgatggaacg aggcgtgatc gaaatcgcac
    62281 ctttagctta catgcggggg cggacattgg atgatgcatt cgttatcttg gacgaagcac
    62341 aaaacacgac agtcgctcag atgaaaatgt tcttgacccg tctcggttat caatcaaaaa
    62401 tgattgtcaa tggggataca agtcagatag acttgcctcg tgggacgaca agtggtcttg
    62461 ttcatgcaga acgaacatta aagcaaatca aaaaaatcga ttttgttaat tttgaagcaa
    62521 gtgacgttgt tcggcaccct gtagttgccg aaatcatcaa agcttatgaa aaggcggatc
    62581 ttcatcaaga ataagctttg cttaggagga taagcacatg ttgaataaaa cactgcagaa
    62641 actacaaaaa aaactcggaa aggcagcatt gcctttcctt ctgttcttgt tttttatcat
    62701 tgtctgcggc gttacattta gtagtgtcaa acaaaaggcc aatgatttca gagaaggcca
    62761 agtagcagaa gagagtatcc gtgcgaataa aacgattgag aacacggaag aaaccgaaca
    62821 aagaagaaaa ctggcagcag aagcagtgac tccagaatat acttatcaaa aagatctggc
    62881 agatgaccag aacaatcgga tcaagcaatt attcgaattg atcgataaga cgaatgatac
    62941 catcaacaaa tcttataatg acaaggtaga taaggcgaag gaagatgaaa atatcccgaa
    63001 acctactaca gaagagcgga ttgctacact gaagaaaaat tttgaaaacg tgaatgcaga
    63061 aaacgttgcg ttttaccaaa aactgccgaa caatttttat tcgacgatat ttgaaatgac
    63121 tgaaacggaa attcacacgg ttagggatga aagtttgaag ctcattgacg aacagatgag
    63181 taaacaagtt cgtgaaagcg aattggaagc ttttaaacaa gaagcagaag atcagattca
    63241 atatttgaat gtcacaacag agcagcaaca aatgattcgc tacttagtgg atcaaggcat
    63301 tgtagttaat gatgtattga atgagaaaaa gacagaagaa ttaaaacaat ctgcacgaga
    63361 agctgtacaa ccagtgatga tcttccaagg agaaattatt gttcgcgaag gaaatcaaat
    63421 tgatgcttca gcgatgaaaa aattggaatt actaggattg acgaatcaaa cgacttctat
    63481 tttcccactc attgccatgg ttttagctgt tttgcttcaa atcggtgtac tgatttataa
    63541 ttcacttcaa ttccaaaatg caggagaaag aacaaaatac gtacttttct acgtgacggc
    63601 aatgtcagta agcgtcgtac taatgaaatt tttccaactc ttccaaacag aacaagctgc
    63661 ttatattccg ctgttttacc ccgcggcttt tgctccactt gttttaagct ttttcttgaa
    63721 tcgaagagca gggatcatgg ctggtatttt tcaagcagta tccgcacttt tcatttttta
    63781 tgattcgatt ggcacgaact ttctgacagt tattttgatg tcctatgtat tttctggtct
    63841 catggcaacg atcgtcagac gcaaacggat ctcagaacaa gggttatccg ctgctatgtg
    63901 ggtcattatt tttcctgtga tgatggatgt cattttgatt atctaccaag gaatgagctt
    63961 tggggatgca acgacttggt taacgctagt atgcggattc acaggtgcac tgttctcatt
    64021 cttgctgacg ataggtctgc acccctatat tgaattgatg gtcaatgatg atagtgtgat
    64081 cgtattaaat gaactcagca atcctaatca tcctttattg aaacagttgc tggaagaagc
    64141 accaggcacg tatcaccata gcatgatggt agccaatcta agtgctaatg ctgtagcaga
    64201 aatcggtgga cgttctttac taactcgagt agcttgctat tatcatgata tcggcaagat
    64261 caaacacgcc agctttttcg ttgagaactt gccttcagga gcggaaaatc cgcacaattt
    64321 cttattgcca gaagacagta aacatattat ctttggacat gtgacagatg gtgccaaaat
    64381 attggaagat tataatatgc cgcaaatggt gatcgatatt tgcagacagc atcatggaac
    64441 cacgctgatg cgttatttct acgttaaggc aaaggaacgc aacccggaag tcacggaaga
    64501 gcaattccgt tatcctggac caaaacctca aagccgtgaa gcaggaatcg tcaatatagc
    64561 tgacagttgt gaagcggcag tacgagcaat ggaccatcca acgagcgaaa aaatcgaaag
    64621 ttttgtccat aatcttattg aagagcgtat ttccgatgga caattggacg acagtggatt
    64681 gacgttaaaa gaaatccgta aagtagaaaa atcattgatc agcggtttaa gcagtacctt
    64741 ccattctcgg atcaaatatc ctaaaatgaa atcggaagtt gagaagataa aagaagaaca
    64801 agaagaatag aaagaagcga tgtataatgg atattacttt catggatgaa acaaaccaag
    64861 tctcagaaga gaaaatgaaa gaaatcgacg acttgctgca atttgcagca tcatatttag
    64921 agttgccaga agaaacagaa atgtctgtta ctttcatgga caatgcagcg atccaagtaa
    64981 tcaatcgtga ttaccgtgga aaagatgcac cgacagacgt catcagtttt gccttggaag
    65041 aagagggcga agacgaaatc ccagtgattt ttgaagatgg agagaatcca ttgcctcgtg
    65101 tactgggaga catcatgatc tctactgaac gtgctgcaga gcaagcagaa gcttacggcc
    65161 attcgtatga tcgggaactt ggctttttag cggttcatgg attcttgcat atcaacgggt
    65221 atgaccatat gacaccagag gatgagaaag aaatgtttgg tttacagaaa gagatcttag
    65281 atgcctatgg acttaaaaga taaaaacgta gagaaaaaca aacatttcct gatgtccgtg
    65341 gaatttgccg tccaaggagt caaaaccgtt tttgaggaag aacgcaacat gaggaaacat
    65401 gttgcgttcg gaatcctcgc tttgtttatg ggcgtgattt tccagcttga tcggatagaa
    65461 tggttatggc tgcttttagc tgtttttctc gtatggatag tagagatcat caatactgtt
    65521 tttgaaaatg tcgtggacat gtttacggat tttcattttc atccgattgg gaaaaaaatc
    65581 aaagatatgg cagctggagc agttttagtg accgcttgtt tttccgtcat cgttgggttg
    65641 attttatttg tccctaaaat atatcatttg ttgtttaatt aaaaaggaga acgtatatgc
    65701 cagaacataa atcagggttt gtcgcaatcg tcggccgccc gaatgtagga aaatccacat
    65761 tattgaatcg aatcgtgggt caaaaaattg ccatcatgag tgataaggcg cagacaacta
    65821 gaaataaaat ccaaggagta tatactacac cagaagctca gttgattttt attgacacac
    65881 caggaatcca taaaccaaag catcgtttag gagattttat ggtagaagca gcttacagtg
    65941 cgttgaaaga agtggacgcg gtcttattta tgatcagcgc tgatcaaaaa cgcggtcggg
    66001 gagacgattt tattattgaa cgtctaaaaa acgtgcattc tcctgtttat ctgattatca
    66061 ataaaatcga taaaatccat ccagacgagt tgttgggaat catcgaagat tattcaacgc
    66121 agatgccgtt tgcacaagtg attccaattt ctgctacaga aggaaacaac gtcgaacgtt
    66181 tgatggatgt attagtcagt gaaatgccag agggcccaca atattttcca gatgatcaag
    66241 tgactgatca tcctgaatac ttcatcgttt ctgaattgat ccgtgagaaa gtcttgtttt
    66301 taacacgaga cgaagtgcct cattctgtcg cagtcgttgt tgattcgatg aagcgaaacg
    66361 agaatgataa aatccatatc caggctacga ttattgtcga aagagatagc caaaaaggga
    66421 tcattatcgg caaaggcgga aaaatgctga agcaagtagg aacgaaagcg agaaaagata
    66481 tcgaaaatct tttaggagac aaagtctttc tagaactttg ggtcaaagtt caaaaagact
    66541 ggcgagacaa aaaagtctat ctgcaagact tcggctaccg aaaagacgaa tattgatatt
    66601 ctgagcttct tgtaagttag gtatagaaat aaactggata aaaatccgcg tgttgagaaa
    66661 agctgcggaa gatcgcaaca taaatgatct ggatcggcag aatctgcttt tccagattta
    66721 ttttttagga gggagaatat gagccagctt gcagaatcaa aaggcttgat tttatctagc
    66781 cgtaattaca aagaaaaaga taaattagtc aaaatcttta cggaatcttc tggcaaaatg
    66841 atgttttata tcaaagggat ccatcggcaa aatcagccct tagctccggc tttgctccct
    66901 tttactgaag cagtttatat cgggaatttt cgaagtgaag gtttgtcatt tttaagcagt
    66961 gtcaaagatg tccacgcgtt tcgacacatc caagaagaca tctttttgag cggctatggc
    67021 acatatttat tgaatctagt agatgcggca atcgaagatc atcagtacga cccgcatttg
    67081 ttccagttta cgcaacaagc actgcaactg atggaccaag gagatgatgc agaaatcatc
    67141 acgaatattt ttgaagtcca aatactgcaa cgcttcggaa ttgcaccaat ctggacacac
    67201 tgcgtcgtat gtggggaaac aaaaggaaaa tttgactatt cttccaaata tggtggtgtg
    67261 atatgcgaaa gacactggtc gatggatgat caccgttatc atgcagatcc tcgagctgtt
    67321 tatttcgttc ggatgttttc agctatttct tatgataaaa tcagtggaat ccatctgaaa
    67381 gaagaaacca aacaagcaat ccgtgaactc atcgaccagc tgtatgatga atatgtcggt
    67441 atccatttga aaagcaaaaa gttcattgat caaatgaagt cttgggcaca cgtattaaag
    67501 acgtcagaga aagacatgtc tgaaaaagat ctacctaaaa aagaagaata gtaaatgaaa
    67561 catacaacaa aaaatcaaaa ccatttgaca aacacaggga gttccgtcta ttattaaccg
    67621 tagatcaaat agatcgttca ctgagaggat ctattataga aacaagaaaa ctgcaaaatg
    67681 aatacgtaca ttgaccaaga tcagtaaaaa acacgtggtt tagcgagttc gggagagtgg
    67741 gagccggacc caaacgattt ttgaagaggc acttgcgagt acgataaatg aagctgccga
    67801 atctttattc ggaagtaggg tggaaccgcg aattattcgt ccctatgtct gtaatcagac
    67861 atagggattt tttgtcgttt tctttcctgt attcttattt gacagcaacg ctttaaaatt
    67921 gaaaggggca aaacagatga gtaaaaaatt gaccgtccaa gatatgattt taacattaca
    67981 aaaattttgg tcatcaaacg gctgcatgct gatgcaagcg tatgacacag aaaaaggagc
    68041 aggtacgatg agcccatata cctttttacg ggcaatcgga ccagaaccat ggaatgctgc
    68101 ttacgtagag ccatctcgcc gaccagctga tggacgttat ggcgaaaacc caaaccgttt
    68161 gtatcagcat catcaattcc aagtcgtgat gaaaccttca cctgagaata tccaagagct
    68221 ttatttagaa agcttgcgcc ttttaggaat cgatccacta gaacacgaca tccgttttgt
    68281 tgaagacaac tgggagaacc catccatggg ctgtgccggt gtgggttggg aagtatggct
    68341 tgacggaatg gaaatcacac aattcacgta cttccaacaa gttggtggac tgccttgtaa
    68401 accagtaact tctgagatca cttatggatt agaacgtctt gcttcttata tccaagaagt
    68461 tgaaagtgtc tatgatttag aatgggctga tggcgtgaaa tatggcgaga tcttcaaaca
    68521 accagaatat gaacattcta aatattcttt tgaagtaagc gaccaagact tgctactaag
    68581 caactttgat cgttttgaaa aagaagctaa acgctgtatc gatgaaaatc ttgttcatcc
    68641 ggcgtatgat tatatcttga aatgtagcca tacattcaac ttattagacg cccgcggtgc
    68701 agtatccgta acagagcgtg ccggttatct ttctcgcatc cgcaacatgg ctcgtgctgt
    68761 agccaaaatc tttgtggcag aacgcgaaaa attaggattc cctttgttga ataaaaacac
    68821 agcaaatcca gaaaacgtga aggaggctga ataagatggc aaaagactta ttacttgaaa
    68881 tcgggctgga agaaatgcct gctcatgttg tcacaccaag catgaaacaa ttagaagaaa
    68941 aaacagcaaa atttttagat gaaaaccaat tgacttatga tacgatcgaa acattttcca
    69001 caccgcgccg tttagcaatc aaagtaacaa acattccaga acgacaagcg gacagtgaag
    69061 aagaagtcaa aggaccagcc aaaaaaattg ctttggacgc agatggaaac tggacgaaag
    69121 cagcggaagg atttgtccgc ggacaaggtc tgacgacaga cgctatcact tttagagaat
    69181 taaaaggtgt ggaatatgtc tatgtaacga aatacactca tggtaaggaa tccaaagaag
    69241 tattgacagg tttagcagat gtcattaaaa gcctgacttt ccctgtaacg atgcattggg
    69301 cagaatacga ttttgaatat atccgcccta ttcactggat cgtggcattg ttagatgacg
    69361 aagtaatccc attcgaaatc ttggatgtca aaacaggcag agcctctcga ggtcatcgtt
    69421 tcttaggcga agatgtgaca tttgcgcatg caaaagaata tgaagaaaaa ttgaatgaac
    69481 aattcgtcat tgcgtctccg caaaaaagac aagaaatgat cgttacacaa gccaatgaat
    69541 tagccgaaaa aaatcattgg gttattgatc tagacgaaga tttactagaa gaagtcaaca
    69601 atttagtcga atatccaact gtcttttctg gcgacttcga agaaaaatat ctttctgttc
    69661 ctgaagaagt actagtcact tctatgaaag aacatcaacg ttatttcgag gtacggaatg
    69721 atcaaggtat gctgcttcct cattttattt ctgctcgtaa tggtgatcgg gtaaaattgg
    69781 aaaatgtggt tcgaggcaat caaaaagtat tgacggctcg tttagaagat gcggaattct
    69841 tttataatga agacaagaaa gcttcaatcg accaatacgt tgaaaaacta aaaactgtta
    69901 ctttccatga aaaaatcggt agtatctatg agaaaatgca acgggtacaa gtcattgccc
    69961 gtatcattgg cgaacaagta ggactatctg aggaagaatt gatggatctg caacgagctg
    70021 ctgaaatcta taaatttgat ctagtaacca acatggtagg ggaatttcct gaactgcaag
    70081 ggatcatggg cgaaaaatat gctctgctga aaggcgagaa acctgctgta gcgacagcga
    70141 ttcgtgagca ttacatgcct atatccagtg aaggagagct acctcagtct acagtaggtt
    70201 ctgttttagc gattgcagat aaattagata gcatcttttc tttctttgct gttgggatga
    70261 tcccaagcgg ttcaaacgat ccttatgcct tacgtcgcca agcatacggt gtcgttcgga
    70321 tcatcgaatc gaaaaaatgg gcctttcctt tgtctgttct gcaagaaacg attagcgatg
    70381 ttatcagtaa agatacggat cgtttcggca ttggtttatc agaagggcaa caacaagtga
    70441 tcgactttat caaaggccgt ctgcgccaat tattgacaac gaagaatatt cgtcatgatg
    70501 tcatcgatgc tgttttgaat gcggaacaaa aagatctgac aaaagtattt gcagcagcac
    70561 aattgttcaa acaacatctt gcagatgaag acttcaaacc ttcaatggaa gctttgacac
    70621 gtgtcgtgaa cttagcgaaa aaagcgcagc ttgaaaaaca atcagaagta gatcctgagc
    70681 tgttcgaaaa cgacgcagag aaagaattac acaaagcagt agaagcaatg aaagcaagtt
    70741 ttgctgacca aacgatccac gaaaactacg accagcttgt cgctcttcgt ccactgatcg
    70801 aaaattattt tgatcaaacg atggtcatgg ctgatgaacc agcagtacgt aagaaccgac
    70861 taacacaact tagtcaaata gctgccatgg ctttgtcatt agcaagcttg gatcaaatca
    70921 tcacaaaata gtcttttgtt tgaaaaacag agagcatgca cgaaacgtgc tgtgtctctg
    70981 tttttttgtt ataaatataa atagctgtta ttaattctat tttaataatt tttgttattt
    71041 tattctttgt tcgaattttc aaaatgctca tgaaaagaga ggaaataaaa atgaaaacga
    71101 ttaatcatga tcttgttact gttttattta cttttaaatt aggaatttcc ctagtcaaga
    71161 aaactttttg tggtatgatt atatagggaa aacctgttta taaatatttc tatgaatata
    71221 ttagtttaat tatgctttta aaataaaaag cgctcattct gatgggaatg aatcacatct
    71281 ttatttatct atcatcaata tttaggagtg aatctatgga aaagccgttc gtcaagttat
    71341 tggctactgt ggcggtaggc acaggcgcta ttgtgatttg cttgtttgga taccatttca
    71401 ataatcagcg gcaacatcat caacggatca attacgcaga atctgccatc accaatcaaa
    71461 aagacaccgt gacctcttta tcaaaagaag tagataagct gtacagtaca aaagaaaaaa
    71521 ttttcctaaa tcctgaaata acagaagaaa cagtttcaaa tttgtcacat aaactatctt
    71581 ctatcaaact ttcggcagat gattatgaca tcaaggaatc agaattacct aaagaagctg
    71641 ctgcgatcca agaagaaaag aaagcagtct taacgcagtt agaggatgca gaatcaaaac
    71701 tgaagatcca gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag
    71761 ctgtagacga tgtcgtcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg
    71821 aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat
    71881 tcgctgatac tcagatcgcg caagtcacgc aactagatca gctattcgat acgatgctga
    71941 aagacggtca agtaacagct actgctactt acgatcaata tttgaccgca ctcagtctca
    72001 tcgaacagat ccggaatgaa aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg
    72061 ctcagcaaat gggctatagc aatacgagct attaataagc gagaattcaa gcaaggctag
    72121 caaacggagg aaactatggc aaaacgaaga agaagatcaa gagggaaaaa gatatttcta
    72181 attattttag caattcttgt cgtattagta ttagcaatcg taggagtagg agcaaaatta
    72241 tatctagatc tctcacattc tattgaaaaa acttacgaat cagtggaacg aacccaagga
    72301 gaacgaacaa aaccagttga tctggacaaa caagaatctt tctctgttct attgatgggg
    72361 attgatacgg gagatttagg tcgggtcgat caaggaagat cagatacgat gatggttgcc
    72421 acagtcagtc ctgaagacaa acagacaacg attgtcagca ttcctcgtga tacgtatgtc
    72481 gacatcgttg gacatgacac aaaagacaag atcaaccacg cttacgcatt cggcggagct
    72541 gccatggcga tggataccgt acaaaaatat gtagacatcc caatcgatca ctatgtttca
    72601 atcaatatga aaggtctaaa agaactcgtt gatgcagttg gtggtatcga agtcaataat
    72661 gacctcacct tctcacaaga tggctatgat tttacgatcg gtaaaatcac attgaacggg
    72721 gatcaggcat tgtcttattc tcgtatgcgt tatgaagatc caaatggtga ctacggcaga
    72781 caagaacgtc aaagaaagat cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt
    72841 gtaagcaact atcaatccgt tttgaatgct ctagagtcga atatgaaaac agacatgagc
    72901 tttgatgaaa tgaaaaagat tgctttagat tatcgggatg catttggtac gatcaagcaa
    72961 gatcagctgc aaggagaagg cttcatgcaa gacaatatct cataccaaaa agtcagcgat
    73021 caagaacttg atcgtgtgaa aaaagaatta aaagatcaaa tgaatctaga gaataaatag
    73081 gaaagtggag aaaaaatgga agagacaatc agcttgaaag aaatttttga aacattacgc
    73141 aaacacgtga taacaatcct catctcaatg tttgctggct tggccatctc aggcatcgtg
    73201 acgtttttca tcttgacgcc aaaatacagc tcacaagccc aattgatcgt gacactgccg
    73261 caatcagata ctgcaaatgt caatgatgtc aatacgaacc tgcaaatgat caatacgtat
    73321 aaggatatga tcgtcagtga tctagtacta aatgaagtca aagatcgttt agaaacagaa
    73381 gacaatgtaa aaatgactgc tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa
    73441 aattcacaaa tgttctcgat ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc
    73501 gctaatacaa cgtcacaagt attccaagag aacgccaaag acgtgatgaa tgtcgataaa
    73561 atctcgatca tctcaggtgc agttgccaat acgacacctg tttcgccaaa taataaattg
    73621 aatctagtga ttggcttagt gttaggaatg atggtaggcg taggtctagc tttcttattt
    73681 gaattactcg accggacagt aaaagatgaa aaatatgtga cggatacatt gggcttcccg
    73741 atcttaggga ctgtaccaga aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa
    73801 ggaccagctc cgacacaacc aaaagctaca aacgataaaa cagcgccatc acgcagaagc
    73861 cgctcaagag tctaggagga aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa
    73921 actcatgcag tcagtttggt cacactcaca aacccctctt caccaatcgc tgaacaatat
    73981 cgtacgattc ggacgaatat ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt
    74041 gttgtgactt catcaggacc tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt
    74101 gtttttgcca aatctggtca acgggtgctg ctagtcgatg cagacttacg taagcccgtc
    74161 atctataaaa cgttcaagtt gaacaacgca agcggattaa gtaccgttct tagttcatca
    74221 ggaagtgtag cagatgagat ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt
    74281 ggacctaaac caccaaatcc atcagaacta ctaagttcgc caagaatgga tcagatttta
    74341 gcagaagcaa gacaactatt tgatgtagtg atcttcgaca tgccgccagt agttgccgta
    74401 acagatgcac agatcatgtc ttccaaaacg gatggcacct tattggtcgt acgtgaaaat
    74461 atgtcacgga aagaatcatt gactaaagca aaagaattgc tggatatggt acaagcgcga
    74521 gtccttggtg ttgtatacaa tggggcagaa cacagcaaag atgctggcta ttactacggg
    74581 aattaagaaa taaaaagaga ggagacaaga gaaatgatcg atctacattg ccatatcctg
    74641 ccaggaatcg atgacggagc agaaaacata gatgccagta tcgccatggc tgaaaaagcg
    74701 attcaacaag ggatcacaca tattttatgc acaccccatc ataataatgg aaaatacagc
    74761 aatgaaaaat cacaagtcat ttctctagtc gcttcgttac aagcagaatt agagaaaaga
    74821 caattgcctt tgacgttatt ggaaggacaa gaagtacgta ttaccggaac actgatcgaa
    74881 gacatcgacc gagatgaaat tttatttaca gatttagatg atacatatct tctaatcgaa
    74941 tttccgacac tggaagtgcc gttatatgca gaacagctct tttttgagtt gttacaacta
    75001 ggaaagacac cagtgatcgt ccatccagaa cgcaacgcgc attttcgtaa agacccgaat
    75061 catatgctgc cattcctaga tatgggatgc ttggcccaac tcaccgcacc aagttatgtc
    75121 ggtgtatttg gaaaagacat ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg
    75181 cagatggctg cttctgatgc ccatggggtc tctaagcgta ccttttactt aaaagagtgc
    75241 tatgagcaga ttgctaagga ctttggtaag gaaaaagtag tacagatgaa acaagtcgca
    75301 aaggatctga tcaacgggga tgaaatcagt
//
LOCUS       DACECS010000006        66911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.006, whole genome shotgun sequence.
ACCESSION   DACECS010000006 DACECS010000000
VERSION     DACECS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.006"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..759)
                     /locus_tag="IWO31_000408"
     CDS             complement(91..759)
                     /locus_tag="IWO31_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0300855.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="IWO31_000409"
     CDS             893..1180
                     /locus_tag="IWO31_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300856.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="IWO31_000410"
     CDS             1596..3134
                     /locus_tag="IWO31_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0300857.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="IWO31_000411"
     CDS             3131..4096
                     /locus_tag="IWO31_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300858.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="IWO31_000412"
     CDS             4083..5405
                     /locus_tag="IWO31_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0300859.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="IWO31_000413"
     CDS             5646..6389
                     /locus_tag="IWO31_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0300860.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="IWO31_000414"
     CDS             6404..7396
                     /locus_tag="IWO31_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0300861.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="IWO31_000415"
     CDS             7408..8145
                     /locus_tag="IWO31_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300862.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="IWO31_000416"
     CDS             8135..8857
                     /locus_tag="IWO31_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0300863.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..9564)
                     /locus_tag="IWO31_000417"
     CDS             complement(8941..9564)
                     /locus_tag="IWO31_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0300864.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            9959..10651
                     /locus_tag="IWO31_000418"
     CDS             9959..10651
                     /locus_tag="IWO31_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0300865.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            10859..11149
                     /locus_tag="IWO31_000419"
     CDS             10859..11149
                     /locus_tag="IWO31_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300866.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            11863..13233
                     /locus_tag="IWO31_000420"
     CDS             11863..13233
                     /locus_tag="IWO31_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300867.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(13473..13556)
                     /locus_tag="IWO31_000421"
     tRNA            complement(13473..13556)
                     /locus_tag="IWO31_000421"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13520..13522),aa:Leu,seq:gag)
     gene            13939..15156
                     /locus_tag="IWO31_000422"
     CDS             13939..15156
                     /locus_tag="IWO31_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0300868.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            15340..16176
                     /locus_tag="IWO31_000423"
     CDS             15340..16176
                     /locus_tag="IWO31_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0300869.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            16252..17400
                     /gene="nagA"
                     /locus_tag="IWO31_000424"
     CDS             16252..17400
                     /gene="nagA"
                     /locus_tag="IWO31_000424"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0300870.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            17620..18678
                     /locus_tag="IWO31_000425"
     CDS             17620..18678
                     /locus_tag="IWO31_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0300871.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            18697..19716
                     /locus_tag="IWO31_000426"
     CDS             18697..19716
                     /locus_tag="IWO31_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0300872.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(19818..20534)
                     /locus_tag="IWO31_000427"
     CDS             complement(19818..20534)
                     /locus_tag="IWO31_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0300873.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(20503..21288)
                     /locus_tag="IWO31_000428"
     CDS             complement(20503..21288)
                     /locus_tag="IWO31_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0300874.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(21279..22028)
                     /locus_tag="IWO31_000429"
     CDS             complement(21279..22028)
                     /locus_tag="IWO31_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0300875.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            22326..22850
                     /locus_tag="IWO31_000430"
     CDS             22326..22850
                     /locus_tag="IWO31_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300876.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            22866..24365
                     /locus_tag="IWO31_000431"
     CDS             22866..24365
                     /locus_tag="IWO31_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0300877.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(24453..25055)
                     /locus_tag="IWO31_000432"
     CDS             complement(24453..25055)
                     /locus_tag="IWO31_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0300878.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            25159..26115
                     /locus_tag="IWO31_000433"
     CDS             25159..26115
                     /locus_tag="IWO31_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0300879.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(26420..27376)
                     /locus_tag="IWO31_000434"
     CDS             complement(26420..27376)
                     /locus_tag="IWO31_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0300880.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            27550..28110
                     /locus_tag="IWO31_000435"
     CDS             27550..28110
                     /locus_tag="IWO31_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300881.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            28103..28927
                     /locus_tag="IWO31_000436"
     CDS             28103..28927
                     /locus_tag="IWO31_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0300882.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            28937..29554
                     /locus_tag="IWO31_000437"
     CDS             28937..29554
                     /locus_tag="IWO31_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0300883.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            29572..30255
                     /locus_tag="IWO31_000438"
     CDS             29572..30255
                     /locus_tag="IWO31_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0300884.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            30938..32173
                     /locus_tag="IWO31_000439"
                     /pseudo
     CDS             30938..32173
                     /locus_tag="IWO31_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            32181..33068
                     /locus_tag="IWO31_000440"
     CDS             32181..33068
                     /locus_tag="IWO31_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0300885.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            33085..33921
                     /locus_tag="IWO31_000441"
     CDS             33085..33921
                     /locus_tag="IWO31_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0300886.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            33924..34547
                     /locus_tag="IWO31_000442"
     CDS             33924..34547
                     /locus_tag="IWO31_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300887.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            34544..35836
                     /locus_tag="IWO31_000443"
     CDS             34544..35836
                     /locus_tag="IWO31_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0300888.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      36195..36292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            36408..36989
                     /locus_tag="IWO31_000444"
     CDS             36408..36989
                     /locus_tag="IWO31_000444"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0300889.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            36992..38299
                     /locus_tag="IWO31_000445"
     CDS             36992..38299
                     /locus_tag="IWO31_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0300890.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            38592..39080
                     /gene="purE"
                     /locus_tag="IWO31_000446"
     CDS             38592..39080
                     /gene="purE"
                     /locus_tag="IWO31_000446"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0300891.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            39064..40197
                     /gene="purK"
                     /locus_tag="IWO31_000447"
     CDS             39064..40197
                     /gene="purK"
                     /locus_tag="IWO31_000447"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0300892.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            40210..41505
                     /gene="purB"
                     /locus_tag="IWO31_000448"
     CDS             40210..41505
                     /gene="purB"
                     /locus_tag="IWO31_000448"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0300893.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(41685..42062)
                     /locus_tag="IWO31_000449"
     CDS             complement(41685..42062)
                     /locus_tag="IWO31_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300894.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            42318..43046
                     /locus_tag="IWO31_000450"
     CDS             42318..43046
                     /locus_tag="IWO31_000450"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0300895.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            43046..43300
                     /gene="purS"
                     /locus_tag="IWO31_000451"
     CDS             43046..43300
                     /gene="purS"
                     /locus_tag="IWO31_000451"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0300896.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            43302..43973
                     /gene="purQ"
                     /locus_tag="IWO31_000452"
     CDS             43302..43973
                     /gene="purQ"
                     /locus_tag="IWO31_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0300897.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            43974..46196
                     /gene="purL"
                     /locus_tag="IWO31_000453"
     CDS             43974..46196
                     /gene="purL"
                     /locus_tag="IWO31_000453"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0300898.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            46181..47620
                     /locus_tag="IWO31_000454"
     CDS             46181..47620
                     /locus_tag="IWO31_000454"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0300899.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            47652..48695
                     /locus_tag="IWO31_000455"
     CDS             47652..48695
                     /locus_tag="IWO31_000455"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0300900.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            48692..49270
                     /gene="purN"
                     /locus_tag="IWO31_000456"
     CDS             48692..49270
                     /gene="purN"
                     /locus_tag="IWO31_000456"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0300901.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            49267..50802
                     /gene="purH"
                     /locus_tag="IWO31_000457"
     CDS             49267..50802
                     /gene="purH"
                     /locus_tag="IWO31_000457"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0300902.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            50818..52092
                     /gene="purD"
                     /locus_tag="IWO31_000458"
     CDS             50818..52092
                     /gene="purD"
                     /locus_tag="IWO31_000458"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0300903.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            52214..53128
                     /locus_tag="IWO31_000459"
     CDS             52214..53128
                     /locus_tag="IWO31_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0300904.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(53261..54826)
                     /locus_tag="IWO31_000460"
     CDS             complement(53261..54826)
                     /locus_tag="IWO31_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0300905.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(54819..56270)
                     /locus_tag="IWO31_000461"
     CDS             complement(54819..56270)
                     /locus_tag="IWO31_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0300906.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(56284..57300)
                     /locus_tag="IWO31_000462"
     CDS             complement(56284..57300)
                     /locus_tag="IWO31_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0300907.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            57520..58608
                     /locus_tag="IWO31_000463"
     CDS             57520..58608
                     /locus_tag="IWO31_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0300908.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            58662..60188
                     /locus_tag="IWO31_000464"
     CDS             58662..60188
                     /locus_tag="IWO31_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300909.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            60190..61395
                     /locus_tag="IWO31_000465"
     CDS             60190..61395
                     /locus_tag="IWO31_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0300910.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            61652..62629
                     /gene="guaC"
                     /locus_tag="IWO31_000466"
     CDS             61652..62629
                     /gene="guaC"
                     /locus_tag="IWO31_000466"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0300911.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            62893..64317
                     /locus_tag="IWO31_000467"
     CDS             62893..64317
                     /locus_tag="IWO31_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0300912.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            64778..65887
                     /gene="pdhA"
                     /locus_tag="IWO31_000468"
     CDS             64778..65887
                     /gene="pdhA"
                     /locus_tag="IWO31_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0300913.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            65890..66867
                     /locus_tag="IWO31_000469"
     CDS             65890..66867
                     /locus_tag="IWO31_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0300914.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRKLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        21628 a        11360 c        14445 g        19478 t
ORIGIN      
        1 ttttttataa agaagatttt actgaataat atcattttag tttacataat gatatttctg
       61 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
      121 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      181 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      241 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      301 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      361 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      421 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      481 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      541 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      601 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      661 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      721 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      781 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
     9361 aatcaggtta atatcagaaa ttgaatttga ttctgcaatg gcattgatca aggagttatc
     9421 accaacgtat ttttgagaaa tcgttgatag agtatcacct gattgaacgg tataagcttc
     9481 gtccgcatga gcagtcgttc ccatgaagaa tgcagcgccg gcagccaaag ttgttccaaa
     9541 aagtaaagtt ttaagtgatg tcatgaaaaa tctcctttat tcgttaaaaa gttttatttg
     9601 ttgtgttcaa cgaggaatac tttaacacgt gaaaatattt cataggtaaa agaagcatga
     9661 caatttatga caaatccgcg ttttttgtaa taaatacagt aatctatgaa atgaaaaggg
     9721 ttacgtttaa taaaacttgc ttttttgttt ttgttcacca taaatattta tttaagaaag
     9781 tattaacctg catttttctc tataaaaaag attttttaaa tagataaaaa aggaggatta
     9841 aatgagtatt tgatagtatt tatattaaaa ttacacatgt aatttaaaaa cgcttgacat
     9901 tgtgtttgag atcacttact tttagcatgt aaaagtattg gattaaggag ctatgcaaat
     9961 gaaaaagaaa tttattactg gtgggattgt cgttgcattt ctatgtctaa ttggctacac
    10021 aggcacgcgg tacgcaaatg aaattatttt gtggggtgga gagacagata ttgatacaat
    10081 caatgagaat ctgacacagc ttgatcatac attgacagat aaagagcaag cgcttacaga
    10141 tcttaccgct cgactagcac aaaaagaaca gacattgcaa gattcaaagg cagatgtcca
    10201 agaataccaa acaaaagtaa atgaattaga aaaccaaaaa aatcaatcgg cagcagaaaa
    10261 acaaaactta gaaagtcaat tagtcagcaa ggatgcacaa ctacaaagca aacaaaatga
    10321 aatcaatgtc aaactagatg agatcaacca aaaaaatgat gaaatcaatc atctgaatgc
    10381 agattggaca gcaagactac aagaagcaca agcaaaactt tcagaattac aaaatactgt
    10441 aaatggtttg aatcaacaaa tcgcaacact gacacaagaa aaaacaaatg tagaaactca
    10501 gttaacagat acacaaaaga aattagacga agcaactgct gaaaactcaa gcttgaaaca
    10561 atatattcaa aagttagaaa aagcaaaaga tgaagtggaa gatacagctg ataaatctca
    10621 gcaaattgtt gaagaacatt tgcgtaaata atctttctta acaagtaaac ctaaattagt
    10681 aaagaagcaa cagtaatcta tactgctgcc tctttttttt agtacatgtc cttcatcttc
    10741 gcaggaagtg ccaatatgat tttaagaaag gtaatagaca aaaagtcaaa aagataaaac
    10801 agagtttctt ctttacaaag ggaatgtatg ttcgtataat ggtattgagg tgaaggttat
    10861 ggagaaaaaa tatggaacag tagataagat ccgtactttg caagttagtg ccagaccgtt
    10921 gatccgtttc agtttaaatg gggtcaattg tttgattgct tcccatagtt tgaacttcct
    10981 agctgaagta gacgaaggga tgaagttagt cgttacaggt tattataata atagaaagca
    11041 atttgttgtg agatcctatc atgtgttagg gaaacccaaa attgtggtag aatatgagaa
    11101 aagtctatac ccaagaaaaa aggtccgaca agatgaaaga ataaactaga aaacaagcgg
    11161 ctgagacaaa aatgtcctag ccgcttgttt tggaaagatg gatgcagacg atgggatttg
    11221 aacccacacg agcatacgct cacaagaacc tcaatctata tataactaca tgtatttaag
    11281 tatttgtgat gaatcactaa actttccaaa ctcatttgag ggatattgct taaagattga
    11341 acagatcaat tcgaagccct attagtattc gctcaatggg gggatcagag gccataaaaa
    11401 gagactagtc attactagtc tcaggtagtg gaattgtagg attttaaacg ttcctggaga
    11461 gtaccggtat gtaactcgaa gaggtgatta tcatagtcgt aaaagtaaat tgaagaagcc
    11521 tcgcctttca cacgagaacg tcctggttcg acagttaaac ctaacatttg gatttttgag
    11581 acaaacgaat ctatatcttg ttcgtcaatt ttaaaagcaa tatgattgta tgtctttggc
    11641 agtttattcg acgaattctg cattactgca atccataggt ccttaactaa gaaaaatttt
    11701 tctggatata atgaaaattt ttttgttgac tatcgtagat ttcttttgca tcaaataagg
    11761 tatttaacaa ttaagtattt aaaagataac ttttaagtta tctttaagta atgcttaaag
    11821 ctatgttata ctctattagg aaatataaga aggagtataa aaatgaacaa acataataga
    11881 aaaataacga ataacaaaga actgatcttg ttactaatag ggatttgggg tatcttttta
    11941 ctctttaata tgtatatgcc gactatgaga gcggatgacg tagtttatgc aagtagatta
    12001 gatgaacttg gctatttggg tgcttcgata gaacattata agacatggag ttcaagaatt
    12061 attatagaac tattcttaat gttcttttct aaacacttta tgctatggaa attattaaat
    12121 tctacgatta tgttaggaat tgtagtatta ctttgcaagt atgtttttga gaaaataaat
    12181 gttaaaaatt tattattggt ttgctctata tactgtttaa taccattgac agtaatgggg
    12241 gaaaccggtt ggagagcaac caccttgaac tatcagtggc cagtagcatt tagtttacta
    12301 actttttatc cgttttttca actcttaaga ggagaagaaa taaatagaaa aatatattgg
    12361 gtaagtattc ctttattgat atttttaact aatcaagaac aagtcaacgc ctgttttttt
    12421 gttctaacaa gtatagttag tttatatttg attgtcaatg gacgatataa ttataagtta
    12481 tcagtatttt caataataag cttagcagaa ttaattttct ctttgacaac tccagggaat
    12541 gcattacgtg ctgcacatga aattaataaa tggtttcctg aatataaaaa tttcaacttt
    12601 cttaataagt tagatttagg tatttcatca tttggaaagc cgtttttttt agacatgaat
    12661 atcttatttt tattgctatt tttcttaatt ttccttctaa catataggaa atgtcaaaac
    12721 tattatgtgc gtatattgac tgcattaccg tttttcttaa acctgattat ttattttggt
    12781 aatacaatgg gtcaaagctt tacttacgtc aatggaaata aaagggcaat gatttggagt
    12841 agtagtaacc tgaataatct ctttacagag ttaggaacga aactgtcact tttttatcca
    12901 ggtacttgga tagctacttt agtagtcttg gcattattac tatgtttaat tgttggaatt
    12961 tatttaagtt ttgacaataa gaaaacttct atttttttag ttattttgat gataatggga
    13021 ttttgttcaa ggttaattat gggattttct cccacagtat gggcttccgg aatgcggacg
    13081 tactatatac tttatgttgt tatcgccatc ttagtactaa tggcagttaa agagctaatg
    13141 aaaagcatga gtgtacaaaa aaatgaattt atgcaatttg gattaaccgt gcttggtatt
    13201 tgtaccttta taataacggt gataaacaga taatatacaa aaaatccctc gctttagtat
    13261 taggtgaggg attttactcg tttttcgatt aattgtttta acacttgcaa gtttctacga
    13321 gtagcttgtt tccgaccttc atatactcac ctcaggctgt ttacatgatt gtacgttctt
    13381 tttgtaaagg atacagtgat atgtatataa tggcataaag aaacgttcaa acaaaaaaac
    13441 tcgtgaagca aaattaactt cacgagttgt agtgcagatg atgggactcg aacccacacg
    13501 agctaacgct cacatgaccc tcaatcatgc ttgtctgcca attccaacac atctgcgtaa
    13561 taataacaca ctaatgatag cgaggtttta catagttgtc aagctcaaaa atgggaggac
    13621 ttattaagat ggagaaatat gacggagaat tttcaggact gggaatgatc ttaggtgtat
    13681 tgattggact tgcattcgga cgttttttgc ttggttttat gctaggaatc atttgtggaa
    13741 ttgcaatgga ttgggcagcg aatctatgga atgactatca tgacaattaa gttctcaacg
    13801 cttgtttgtg tttttttaat acttacgttt ttcctaaata agtggcaaaa ttttctagga
    13861 tggagtatga tattgaaata gtaaataaca aaaaatttgg aaagttctaa ctttttgaaa
    13921 tgagggattg gctaagtgat gagagaattt gaaaaatcca acaaattaga aggtgtcagt
    13981 tacgatgttc gcggtcccgt tctagaagaa gcggagcgga tgcaagaaga gggcattagt
    14041 attttaaaat tgaacacggg aaatcccgca ccttttggat ttgaagcacc aaacgaagtc
    14101 attcgtgatt tgatcatgaa tgcccgtgac tcagaaggct actcagattc caaggggatc
    14161 ttttctgcta gaaaagcaat tgagcaatat tgccaactga aacattttcc gaatgtgacg
    14221 atcaatgata tctacaccgg caacggagtg agcgaattga tcacgatgtg tatgcagggg
    14281 ttgctggaca atggagatga agtactggtt ccaatgccgg actatccgtt gtggacggct
    14341 tctattgcat tagctggagg cacacctgtt cattatattt gcgatgaaga ggcagaatgg
    14401 tatcctgata ttgatgatat caaatcaaaa ataacgagtc ggacaaaagc tattgttatc
    14461 attaatccta ataatcctac tggggcactt tatccaaaag aattattaga agaaatcgtt
    14521 gaagtagctc gacaaaataa cctgattatt tattccgacg aaatctatga tcgcttagtg
    14581 atggatggtc tagaacatat tccgattgct acattagcgc ctgatttgtt cgttgttacg
    14641 ctgaacggtc tatccaaatc tcatcgtgta gcaggatttc gagtaggctg gatggtccta
    14701 agtggagata agtcacacgt caaaggctat atagaagggc tgaatatgtt gtcctccatg
    14761 cgtttgtgtt ccaatgtatt gtctcagcaa attatccaaa ccgcgctagg cggttatcaa
    14821 agtgtcgatg agctgctcct gccaggcgga aggatctatg aacaaagaga atatatctat
    14881 aatgcaatca atgatattcc tggtctttca gccgtaaaac caaaagctgc tttttatata
    14941 ttcccaaaaa ttgatacaaa gcgattcaat attttggatg atgaaaaatt cgttttggac
    15001 tttttacatg aacatcatat tctacttgta catggtggtg gatttaattg gaaccagcca
    15061 gatcattttc gaatcgttta tcttcctaaa atggatgatt tgaaacaaac agctaagaaa
    15121 atgagagaat tcctagcaac ctatcgccaa aaatgatttt aaaaaccaaa tttttcagta
    15181 ggagggccac agacaaacac actgaggctc tctttctagt ggagttttct taaataacta
    15241 aagtatatta tttagttgca agtcttttct tgaagtttgc gacttcctaa agtaaaatac
    15301 gaatagaatg cgaatacgca tattttgggg aggatactta tggaaacgtt aatcgagtcc
    15361 tttggaacga tcattaaaga gattcgaaaa gaacaaaaaa tgacgcaaaa aatgctttcg
    15421 catgatattt gttcacaaag tgtattaagt cggatcgaaa ataatgagga actgccgaat
    15481 gtattggtga tgatgcagat ttgtcaacgg ctaggggtaa cgattgatca tgtgatgaat
    15541 ctctctcgaa aaactactcg gacaaacgat aaattatttg aactgttgga tagttgtttc
    15601 caaaaacgtg attatcggaa gttagaacaa gtactaaaat cttctgatat ttctgaaaat
    15661 ctgtatcaag caactgactt tcaacgctat tattattatc tcggtgtttg cgaattcacg
    15721 ttgcgtcaaa atatctctca gtccctttat tatttgaaag aagcattaag ctactctagc
    15781 caaaaagaaa gaatccatgt ttctgacaca gaaatacaac tgattagttg tatcggaaaa
    15841 atatatggtg tggcaggaaa aacggctgaa gcacgctact atttgagtcg aagcatccaa
    15901 ctgctgcatc aatcagctga tgaacgatca aaaagtgaat tttcgcaaat tttttataat
    15961 tatggaaatt tcttattcca tcaaaatgaa atcgacgaag cgttagaaca ggtgaatcaa
    16021 ggaatctatt gggctcaaga gaagaatagc tactattacc tcaatgattt atttgtgctt
    16081 aaaagcttaa tatacaagcg taaagaagaa ccagaaagag cagcattcta tgaaggctta
    16141 gcacaagctg tgaaacaaat ttcaaaaaat ttgtaaatgg tgtagaccat tcttgacttc
    16201 ttctctaaat aagagtactt tagaggtaga aaaacaaagg gagagaatag aatgagaact
    16261 tttatctatg ctgacaaatt ctttttagcg tcaggtgtca aacatgcagg ctatcttgaa
    16321 attaccgatg gactttttgg tgaatacacc aaagaactac ctgagggaga agtcaacatc
    16381 attgaccaaa gcggcaaatg gattgctcct ggtttggtag atacacatat tcacggctat
    16441 atgaatcatg atgtaatgga taatgatgca gaaggcatca aagtgatgtc tgaaggtctg
    16501 ttatcttgtg gagtgacttc atttttacca acaacattga cgtcaagcaa agaacgttta
    16561 aaagatgtag ctgagacaat cggcaaaatg tatcaggaag caccaggagc taaaatcaaa
    16621 ggaatctatt ttgaaggtcc tttcttcaca gaagaacaca aaggggcaca aaacccaagt
    16681 tatttcgggg atcctgattt ggatactttc catgaatggc aagaagcttc aggtggttta
    16741 atcaaaaaaa ttgctttagc accggaacgt caaggtgtag aagagtttgt caaacaagtg
    16801 actgaagaag gagtagttgt ttctctagga cacagtaatg ctacactaga agaagcgcaa
    16861 aaagctgttg aagcaggcgc aagtgtattt gtccatgctt tcaatggtat gcgtggattg
    16921 aatcatcgcg aaccaggtat ggtgggtgcg ttattatctt tagaacatgt cttttctgaa
    16981 ttgatttgtg atggccatca tgtacatcca aatgctgcgg gaatcctaat ggaaaaagca
    17041 ggacatgatc atgtcgcatt aattacagac tgtatgatgg ccggtgggat gcctgatgga
    17101 aattacaatt taggcgaatt tccagtagta gtaaaagaag gaacagcacg tctagaatct
    17161 ggcaacttag ctggaagtat attgaaattg aaagaagcaa ttaaaaatgt tgtagactgg
    17221 ggattagcaa caccagaaca agcagtcatg atggctagtc tgattccagc aatcagctgt
    17281 aaaatcgatg atacatgtgg aaaaatcgct caaggacgcg atgcagactt tatcgtattg
    17341 aaccctgata tgacattgga agcaacttat ctagatggcg tggaacgttt ccacgcataa
    17401 tataatttaa gttaacctta aaactaaact tcttaagttt ggttttaagg tttttttgtg
    17461 ttctagtaaa aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat
    17521 aagtaacaat gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt
    17581 ttttattgtg aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt
    17641 cgatcgactt tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata
    17701 ttattagggt ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta
    17761 tcgaaagtcg tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat
    17821 atcaatacag aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat
    17881 attattctta ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca
    17941 acgttgcgtg tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt
    18001 gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc
    18061 tttttgtatt cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc
    18121 aaagcgatgt tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg
    18181 ttccaagggt atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta
    18241 gcgtttttgc gaattgacca gactgcaccg tggctatttc agccgatgga ttatttagct
    18301 ggtttaagtt caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc
    18361 aaaaaagctg catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta
    18421 cctgcattga atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat
    18481 tttgaagcac tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca
    18541 gcctatgcta tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca
    18601 actatttttg ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc
    18661 ataggtttat tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt
    18721 cgtccgaacg agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt
    18781 tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg
    18841 ttgaggggaa attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt
    18901 cgttatgttt ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat
    18961 tttggtatga cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct
    19021 ttgacttttg ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc
    19081 aaagattttc gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg
    19141 atcataggaa caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc
    19201 aaagtgatcg gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac
    19261 tcgcttgaag agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca
    19321 ggtgaaaacg atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc
    19381 ttgattaata cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa
    19441 agcaatcatt tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc
    19501 cgatccttca agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg
    19561 taccctagag ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat
    19621 atgatttcga caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat
    19681 gaggtatctc ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa
    19741 aatgtttaaa aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta
    19801 tgggataatt cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat
    19861 tcataacagg ttagcggctg gccgatttcc aaatatttga ttctttcgtt caatatatct
    19921 tgttctacta aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga
    19981 gccagttctt ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt
    20041 aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga
    20101 gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt
    20161 gtctgagaga aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt
    20221 ttgatcaaat aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct
    20281 gcagcagtga tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa
    20341 tggataccac tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt
    20401 ctgaaatatt tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg
    20461 atgctttcca caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg
    20521 acatttgtca tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc
    20581 atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag
    20641 attcaatcca aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc
    20701 tgagatttct tctgatatcc cgacaccgcc atcgcagacg gtcacgatga atacttcagc
    20761 ttctgtgtca tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac
    20821 ggcttctttt gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc
    20881 atccatctcc attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc
    20941 tttggcttcg tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact
    21001 tgagacttgg ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac
    21061 ttcttcatac ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc
    21121 atgggtttga gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa
    21181 tttcattact tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct
    21241 aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg
    21301 aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat
    21361 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
    21421 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
    21481 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
    21541 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
    21601 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
    21661 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
    21721 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
    21781 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
    21841 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
    21901 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
    21961 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
    22021 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
    22081 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
    22141 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
    22201 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
    22261 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
    22321 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
    22381 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
    22441 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
    22501 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
    22561 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
    22621 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
    22681 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
    22741 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
    22801 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
    22861 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
    22921 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
    22981 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
    23041 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
    23101 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
    23161 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
    23221 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
    23281 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
    23341 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
    23401 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
    23461 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
    23521 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
    23581 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
    23641 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
    23701 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
    23761 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
    23821 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
    23881 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
    23941 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
    24001 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
    24061 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
    24121 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
    24181 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
    24241 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
    24301 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
    24361 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
    24421 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
    24481 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
    24541 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
    24601 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
    24661 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
    24721 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
    24781 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
    24841 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
    24901 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
    24961 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
    25021 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
    25081 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
    25141 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
    25201 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
    25261 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
    25321 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
    25381 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
    25441 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
    25501 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
    25561 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
    25621 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
    25681 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
    25741 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
    25801 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
    25861 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
    25921 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
    25981 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
    26041 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
    26101 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
    26161 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
    26221 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
    26281 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
    26341 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
    26401 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
    26461 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
    26521 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
    26581 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
    26641 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
    26701 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
    26761 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
    26821 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
    26881 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
    26941 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
    27001 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
    27061 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
    27121 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
    27181 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
    27241 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
    27301 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
    27361 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
    27421 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
    27481 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
    27541 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
    27601 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
    27661 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
    27721 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
    27781 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
    27841 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
    27901 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
    27961 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
    28021 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
    28081 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
    28141 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
    28201 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
    28261 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
    28321 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
    28381 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
    28441 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
    28501 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
    28561 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
    28621 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
    28681 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
    28741 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
    28801 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
    28861 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
    28921 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
    28981 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
    29041 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
    29101 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
    29161 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
    29221 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
    29281 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
    29341 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
    29401 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
    29461 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
    29521 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
    29581 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
    29641 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
    29701 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
    29761 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
    29821 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
    29881 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
    29941 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
    30001 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
    30061 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
    30121 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
    30181 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
    30241 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
    30301 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
    30361 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
    30421 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
    30481 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
    30541 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
    30601 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
    30661 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
    30721 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
    30781 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
    30841 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
    30901 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
    30961 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
    31021 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
    31081 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
    31141 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
    31201 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
    31261 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
    31321 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    31381 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    31441 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    31501 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    31561 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    31621 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    31681 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    31741 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    31801 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    31861 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    31921 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    31981 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    32041 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    32101 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    32161 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    32221 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    32281 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    32341 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    32401 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    32461 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    32521 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    32581 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    32641 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    32701 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    32761 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    32821 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    32881 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    32941 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    33001 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    33061 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    33121 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    33181 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    33241 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    33301 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    33361 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    33421 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    33481 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    33541 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    33601 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    33661 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    33721 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    33781 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    33841 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    33901 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    33961 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    34021 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    34081 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    34141 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    34201 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    34261 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    34321 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    34381 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    34441 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    34501 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    34561 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    34621 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    34681 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    34741 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    34801 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    34861 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    34921 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    34981 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    35041 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    35101 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    35161 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    35221 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    35281 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    35341 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    35401 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    35461 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    35521 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    35581 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    35641 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    35701 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    35761 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    35821 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    35881 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    35941 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    36001 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    36061 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    36121 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    36181 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    36241 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    36301 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    36361 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    36421 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    36481 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    36541 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    36601 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    36661 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    36721 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    36781 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    36841 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    36901 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    36961 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    37021 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    37081 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    37141 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    37201 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    37261 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    37321 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    37381 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    37441 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    37501 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    37561 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    37621 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    37681 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    37741 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    37801 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    37861 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    37921 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    37981 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    38041 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    38101 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    38161 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    38221 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    38281 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    38341 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    38401 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    38461 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    38521 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    38581 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    38641 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    38701 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    38761 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    38821 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    38881 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    38941 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    39001 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    39061 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    39121 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    39181 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    39241 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    39301 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    39361 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    39421 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    39481 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    39541 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    39601 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    39661 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    39721 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    39781 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    39841 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    39901 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    39961 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    40021 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    40081 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    40141 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    40201 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    40261 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    40321 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    40381 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    40441 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    40501 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    40561 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    40621 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    40681 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    40741 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    40801 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    40861 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    40921 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    40981 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    41041 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    41101 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    41161 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    41221 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    41281 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    41341 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    41401 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    41461 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    41521 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    41581 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    41641 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    41701 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    41761 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    41821 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    41881 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    41941 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    42001 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    42061 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    42121 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    42181 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    42241 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    42301 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    42361 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    42421 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    42481 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    42541 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    42601 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    42661 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    42721 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    42781 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    42841 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    42901 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    42961 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    43021 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    43081 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    43141 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    43201 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    43261 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    43321 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    43381 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    43441 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    43501 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    43561 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    43621 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    43681 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    43741 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    43801 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    43861 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    43921 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    43981 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    44041 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    44101 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    44161 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    44221 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    44281 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    44341 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    44401 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    44461 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    44521 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    44581 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    44641 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    44701 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    44761 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    44821 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    44881 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    44941 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    45001 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    45061 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    45121 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    45181 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    45241 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    45301 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    45361 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    45421 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    45481 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    45541 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    45601 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    45661 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    45721 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    45781 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    45841 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    45901 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    45961 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    46021 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    46081 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    46141 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    46201 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    46261 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    46321 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    46381 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    46441 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    46501 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    46561 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    46621 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    46681 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    46741 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    46801 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    46861 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    46921 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    46981 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    47041 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    47101 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    47161 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    47221 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    47281 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    47341 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    47401 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    47461 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    47521 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    47581 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    47641 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    47701 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    47761 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    47821 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    47881 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    47941 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    48001 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    48061 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    48121 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    48181 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    48241 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    48301 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    48361 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    48421 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    48481 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    48541 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    48601 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    48661 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    48721 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    48781 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    48841 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    48901 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    48961 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    49021 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    49081 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    49141 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    49201 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    49261 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    49321 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    49381 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    49441 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    49501 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    49561 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    49621 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    49681 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    49741 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    49801 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    49861 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    49921 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    49981 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    50041 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    50101 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    50161 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    50221 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    50281 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    50341 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    50401 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    50461 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    50521 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    50581 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    50641 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    50701 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    50761 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    50821 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    50881 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    50941 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    51001 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    51061 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    51121 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    51181 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    51241 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    51301 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    51361 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    51421 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    51481 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    51541 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    51601 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    51661 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    51721 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    51781 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    51841 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    51901 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    51961 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    52021 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    52081 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    52141 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    52201 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    52261 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    52321 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    52381 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    52441 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    52501 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    52561 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    52621 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    52681 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    52741 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    52801 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    52861 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    52921 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    52981 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    53041 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    53101 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    53161 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    53221 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    53281 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    53341 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    53401 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    53461 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    53521 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    53581 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    53641 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    53701 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    53761 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    53821 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    53881 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    53941 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    54001 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    54061 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    54121 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    54181 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    54241 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    54301 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    54361 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    54421 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    54481 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    54541 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    54601 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    54661 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    54721 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    54781 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    54841 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    54901 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    54961 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    55021 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    55081 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    55141 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    55201 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    55261 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    55321 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    55381 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    55441 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    55501 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    55561 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    55621 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    55681 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    55741 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    55801 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    55861 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    55921 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    55981 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    56041 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    56101 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    56161 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    56221 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    56281 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    56341 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    56401 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    56461 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    56521 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    56581 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    56641 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    56701 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    56761 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    56821 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    56881 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    56941 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    57001 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    57061 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    57121 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    57181 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    57241 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    57301 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    57361 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    57421 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    57481 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    57541 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    57601 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    57661 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    57721 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    57781 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    57841 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    57901 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    57961 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    58021 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    58081 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    58141 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    58201 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    58261 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    58321 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    58381 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    58441 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    58501 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    58561 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    58621 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    58681 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    58741 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    58801 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    58861 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    58921 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    58981 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    59041 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    59101 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    59161 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    59221 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    59281 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    59341 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    59401 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    59461 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    59521 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    59581 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    59641 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    59701 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    59761 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    59821 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    59881 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    59941 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    60001 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    60061 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    60121 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    60181 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    60241 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    60301 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    60361 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    60421 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    60481 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    60541 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    60601 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    60661 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    60721 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    60781 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    60841 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    60901 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    60961 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    61021 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    61081 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    61141 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    61201 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    61261 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    61321 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    61381 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    61441 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    61501 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    61561 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    61621 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    61681 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    61741 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    61801 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    61861 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    61921 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    61981 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    62041 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    62101 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    62161 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    62221 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    62281 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    62341 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    62401 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    62461 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    62521 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    62581 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    62641 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    62701 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    62761 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    62821 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    62881 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    62941 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    63001 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    63061 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    63121 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    63181 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    63241 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    63301 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    63361 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    63421 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    63481 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    63541 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    63601 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    63661 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    63721 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    63781 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    63841 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    63901 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    63961 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    64021 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    64081 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    64141 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    64201 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    64261 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    64321 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    64381 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    64441 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    64501 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    64561 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    64621 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    64681 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    64741 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    64801 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    64861 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    64921 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    64981 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    65041 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    65101 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    65161 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    65221 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    65281 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    65341 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    65401 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    65461 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    65521 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    65581 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    65641 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    65701 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    65761 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    65821 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    65881 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    65941 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    66001 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    66061 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    66121 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    66181 atggctcgta caagatatcg aatgagcggc actagaaaac tacccatcac aattcgttcg
    66241 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    66301 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    66361 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    66421 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    66481 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    66541 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    66601 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    66661 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    66721 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    66781 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    66841 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    66901 caatttaaat t
//
LOCUS       DACECS010000007        62215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.007, whole genome shotgun sequence.
ACCESSION   DACECS010000007 DACECS010000000
VERSION     DACECS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.007"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..761)
                     /locus_tag="IWO31_000470"
     CDS             complement(138..761)
                     /locus_tag="IWO31_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300915.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(920..2317)
                     /locus_tag="IWO31_000471"
     CDS             complement(920..2317)
                     /locus_tag="IWO31_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0300916.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2349..3320)
                     /locus_tag="IWO31_000472"
     CDS             complement(2349..3320)
                     /locus_tag="IWO31_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0300917.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3694..4119
                     /locus_tag="IWO31_000473"
     CDS             3694..4119
                     /locus_tag="IWO31_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300918.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4607..5308)
                     /locus_tag="IWO31_000474"
     CDS             complement(4607..5308)
                     /locus_tag="IWO31_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0300919.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5324..5764)
                     /locus_tag="IWO31_000475"
     CDS             complement(5324..5764)
                     /locus_tag="IWO31_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0300920.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5764..6339)
                     /locus_tag="IWO31_000476"
     CDS             complement(5764..6339)
                     /locus_tag="IWO31_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0300921.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7187..7666)
                     /locus_tag="IWO31_000477"
                     /pseudo
     CDS             complement(7187..7666)
                     /locus_tag="IWO31_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7825..8526)
                     /locus_tag="IWO31_000478"
     CDS             complement(7825..8526)
                     /locus_tag="IWO31_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0300922.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8634..10043)
                     /locus_tag="IWO31_000479"
     CDS             complement(8634..10043)
                     /locus_tag="IWO31_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0300923.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(10091..11956)
                     /locus_tag="IWO31_000480"
     CDS             complement(10091..11956)
                     /locus_tag="IWO31_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0300924.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(12077..12928)
                     /locus_tag="IWO31_000481"
     CDS             complement(12077..12928)
                     /locus_tag="IWO31_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0300925.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(13238..13951)
                     /locus_tag="IWO31_000482"
     CDS             complement(13238..13951)
                     /locus_tag="IWO31_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0300926.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(14019..14345)
                     /locus_tag="IWO31_000483"
     CDS             complement(14019..14345)
                     /locus_tag="IWO31_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0300927.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(14468..14695)
                     /locus_tag="IWO31_000484"
     CDS             complement(14468..14695)
                     /locus_tag="IWO31_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0300928.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(15017..16270)
                     /locus_tag="IWO31_000485"
     CDS             complement(15017..16270)
                     /locus_tag="IWO31_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0300929.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16331..17071
                     /locus_tag="IWO31_000486"
     CDS             16331..17071
                     /locus_tag="IWO31_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0300930.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(17157..17915)
                     /locus_tag="IWO31_000487"
     CDS             complement(17157..17915)
                     /locus_tag="IWO31_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0300931.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(17912..18940)
                     /locus_tag="IWO31_000488"
                     /pseudo
     CDS             complement(17912..18940)
                     /locus_tag="IWO31_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(18960..22127)
                     /locus_tag="IWO31_000489"
     CDS             complement(18960..22127)
                     /locus_tag="IWO31_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300932.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(22351..23235)
                     /locus_tag="IWO31_000490"
     CDS             complement(22351..23235)
                     /locus_tag="IWO31_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0300933.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(23330..23767)
                     /locus_tag="IWO31_000491"
     CDS             complement(23330..23767)
                     /locus_tag="IWO31_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300934.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(23984..24895)
                     /locus_tag="IWO31_000492"
     CDS             complement(23984..24895)
                     /locus_tag="IWO31_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0300935.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(25352..25780)
                     /locus_tag="IWO31_000493"
     CDS             complement(25352..25780)
                     /locus_tag="IWO31_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300936.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(25808..26446)
                     /locus_tag="IWO31_000494"
     CDS             complement(25808..26446)
                     /locus_tag="IWO31_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0300937.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(26450..28486)
                     /locus_tag="IWO31_000495"
     CDS             complement(26450..28486)
                     /locus_tag="IWO31_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0300938.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            28612..29277
                     /locus_tag="IWO31_000496"
     CDS             28612..29277
                     /locus_tag="IWO31_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0300939.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(31212..34361)
                     /locus_tag="IWO31_000497"
     CDS             complement(31212..34361)
                     /locus_tag="IWO31_000497"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0300940.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(34374..35591)
                     /locus_tag="IWO31_000498"
     CDS             complement(34374..35591)
                     /locus_tag="IWO31_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0300941.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(35581..37176)
                     /locus_tag="IWO31_000499"
     CDS             complement(35581..37176)
                     /locus_tag="IWO31_000499"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0300942.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(37686..38468)
                     /locus_tag="IWO31_000500"
     CDS             complement(37686..38468)
                     /locus_tag="IWO31_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300943.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(38533..39369)
                     /locus_tag="IWO31_000501"
     CDS             complement(38533..39369)
                     /locus_tag="IWO31_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0300944.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(39387..40796)
                     /locus_tag="IWO31_000502"
     CDS             complement(39387..40796)
                     /locus_tag="IWO31_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0300945.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(40810..42633)
                     /locus_tag="IWO31_000503"
     CDS             complement(40810..42633)
                     /locus_tag="IWO31_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0300946.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(43476..43802)
                     /locus_tag="IWO31_000504"
     CDS             complement(43476..43802)
                     /locus_tag="IWO31_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300947.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(43848..44210)
                     /locus_tag="IWO31_000505"
     CDS             complement(43848..44210)
                     /locus_tag="IWO31_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0300948.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(44247..44462)
                     /locus_tag="IWO31_000506"
     CDS             complement(44247..44462)
                     /locus_tag="IWO31_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0300949.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            44589..44924
                     /locus_tag="IWO31_000507"
     CDS             44589..44924
                     /locus_tag="IWO31_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0300950.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(45000..46997)
                     /locus_tag="IWO31_000508"
     CDS             complement(45000..46997)
                     /locus_tag="IWO31_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0300951.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(47095..47370)
                     /locus_tag="IWO31_000509"
     CDS             complement(47095..47370)
                     /locus_tag="IWO31_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0300952.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            47602..48336
                     /locus_tag="IWO31_000510"
     CDS             47602..48336
                     /locus_tag="IWO31_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0300953.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            48375..49517
                     /locus_tag="IWO31_000511"
     CDS             48375..49517
                     /locus_tag="IWO31_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0300954.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(49612..51003)
                     /gene="sufB"
                     /locus_tag="IWO31_000512"
     CDS             complement(49612..51003)
                     /gene="sufB"
                     /locus_tag="IWO31_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0300955.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(51008..51478)
                     /locus_tag="IWO31_000513"
     CDS             complement(51008..51478)
                     /locus_tag="IWO31_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0300956.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(51465..52700)
                     /locus_tag="IWO31_000514"
     CDS             complement(51465..52700)
                     /locus_tag="IWO31_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0300957.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(52697..53983)
                     /gene="sufD"
                     /locus_tag="IWO31_000515"
     CDS             complement(52697..53983)
                     /gene="sufD"
                     /locus_tag="IWO31_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0300958.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(53996..54769)
                     /gene="sufC"
                     /locus_tag="IWO31_000516"
     CDS             complement(53996..54769)
                     /gene="sufC"
                     /locus_tag="IWO31_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0300959.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(54966..55814)
                     /locus_tag="IWO31_000517"
     CDS             complement(54966..55814)
                     /locus_tag="IWO31_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0300960.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(55838..56524)
                     /locus_tag="IWO31_000518"
     CDS             complement(55838..56524)
                     /locus_tag="IWO31_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0300961.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(56517..57551)
                     /locus_tag="IWO31_000519"
     CDS             complement(56517..57551)
                     /locus_tag="IWO31_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0300962.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(57946..58284)
                     /locus_tag="IWO31_000520"
     CDS             complement(57946..58284)
                     /locus_tag="IWO31_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0300963.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(58286..58642)
                     /locus_tag="IWO31_000521"
     CDS             complement(58286..58642)
                     /locus_tag="IWO31_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0300964.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(58648..59835)
                     /locus_tag="IWO31_000522"
     CDS             complement(58648..59835)
                     /locus_tag="IWO31_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0300965.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(60026..60871)
                     /locus_tag="IWO31_000523"
     CDS             complement(60026..60871)
                     /locus_tag="IWO31_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0300966.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            61105..62070
                     /locus_tag="IWO31_000524"
     CDS             61105..62070
                     /locus_tag="IWO31_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0300967.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        18118 a        12912 c        10291 g        20894 t
ORIGIN      
        1 tcaagacctc ttgatacacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg
       61 gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca aaacgctgat
      121 ttcacaacct ctgtttttca ttgtccatag gattgatatt gtccttgtga tgactcattc
      181 gtaataattg aggcttgatt ttcttcactt cccgttatcg tttgtccact gccagaatct
      241 gaaggacttg ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt cttgacttgt
      301 tcatttgtct ttttccattc cgctaaacga tcggcgacca cttttttgtc tactttttca
      361 gaataagcgg tgatcacttt tccatttttc aaacgggcta ctttgtcgcc atcatgagtc
      421 aaaagcaaaa cggtcaaatg cttgtcttta aattgatatt gtctgagaga aatcgcacta
      481 agtacgtcat cgatattaat aaattgattt tcttcttttc cttttggcaa ataaatgtcc
      541 aattcgcctt tttcgtaatt ggcagttatt ttatactccg cccaccaata agttgagaat
      601 tcttttttga aattatctac tgattctttg cttgctacga ttttttctgt ttctttctga
      661 gcaagatctg ctgtcatttt tcctgaaaat gctgcggata aaataagcag taagaaaaaa
      721 ccgaataacc aaaagagaag aaacggtctt tttttcctca tgctttcctc cactcctatt
      781 taattttgac tattctatta tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa
      841 gatcgtaaat aattgaactt ctttttcaca aaatggtaag aatggagaat ttcacgcatt
      901 tgctctcaac tattctttct taagcctcaa atccgttttt atctgatacg tctttgaacc
      961 aatgacccga ttttttgatt gttctttttc catcttgtgc taagtctact gaaataaagc
     1021 catatcgatt cttatatgca ttggtccacg accagttatc catacatgtc cacatatgat
     1081 agcctaaaca attggcacct tcttgaatgg cttgatggac atatttcaga tgagattgga
     1141 caaattcgat ccgataatca tcgtggatca tgccatcttc tttgataaat cgtgcttcgt
     1201 tttctacacc cataccgttt tcagaaataa agcagtcgat atttccataa tgatctcgta
     1261 cattgatcaa aatatcatag attccttgtt catagatctc ccatccgcga taaggattca
     1321 ttttacgacc aggcatttca taattgtcga aataatcctc aggcattgga ccgtttttag
     1381 tatcgattgg tgtttctttt gcttttaccc ggcgtggctg gtaataattg atacctaaaa
     1441 gatctactgt attttctttg ataaccgtca aatcctctgc atggatcaca ggcatgagat
     1501 caagttcttt tactatctca atcaattcac ttggaaactc gccttttaca gctggatcaa
     1561 ggaatgaacg attgaagaac gcatctgcta tttgcgctgc tttaacatct tcagggtttg
     1621 attcgtctct tggatagctt ggtgtcagat tcaagatgat accaataaca ccaccttgct
     1681 ccatttcatg gtaagatttg atggctaacg ctgaagccaa cgcttcatga tagccaactt
     1741 ggatcgcttt tttcatatct gactcttctg gataatgcca tcgatataaa taacctgctt
     1801 cgactggaac gattggttca ttatgagtaa accatttttt tactttcgaa ccaaacaatt
     1861 caaaacaact tttggcatat ttttcatagg cttcaaccgt ttctcggttc aaccatccgc
     1921 ctttttcttg cagatacatc ggcatatcga aatgatacag attcacaaag ggttcgatac
     1981 cgttttcaat caactcatca aaataacgat gatagaaacg aacggcttct tcgttgattg
     2041 ttccagattc atcaggtatc aaacggctcc attggataga tgttcgaaat gaattatgcc
     2101 cagtctgttt catcaactgt acatcctctt gatacttcgt ataaacttgc gaggtttttt
     2161 caggtcctac ttgatcaaag aacttttgcg gttctttttt gtaccaataa tcaaaaatgt
     2221 tctctccttt tccatcgcct gaaatccttc cttctgtttg tgggccactt gctgcagaac
     2281 cccaccaaaa atttgctgga aattgatatt ttttcatcaa agcacctctt tttctttttt
     2341 agttcgtttt agttgattcc atgtatgata ttttccatca caatcacttt ttcattttct
     2401 tcctcttctt ttttgattcg ttttaacagc tgttctgctg ctcgttcccc gattttatct
     2461 gttggtggcg tgatcgtgat tttcggaagg atactcagat cgtttttatc taaataatca
     2521 tagtgactga acgcaatatc cttcccgatt ctgacacctt cttgattgaa acattcgatg
     2581 gcaccaatcg acatattgta attgctgaca aaaatagcag ttgccccttt ttctaataat
     2641 gcttttgccc cgtaatagcc gctttttcta gaatagtctc ctgtaaagac ctctaattgg
     2701 atcgatggat cattttggac cgcttctttg gctcctgcta aacgttctct tgctacgtag
     2761 tctgtttgag tggcagcaat aaatccgatt tttttatgtc cttgttcacc atgatgagcg
     2821 atcacttttg ctacactttc tcgatcattc actaatattg tatcaatacc tgctggtcct
     2881 tctggacaat tcaatgcgat aactggaata ctcatttttg tgagcatttc aaatccagac
     2941 cactcttctt cggataagaa gacaatcagc ccatcgatca catgcttcat caggtattca
     3001 attttttgtt caattttttc tggctgatcg gcaaacccgc ttaataataa gctataccca
     3061 ttttcttcag ctactctttc gatccctgag acaacttccg acccaaaacg gctggaaatc
     3121 ccgtttatca aaagtccaat agagaagctt cgattattct tcaacccttt agcaatgatg
     3181 ttttctttat agtccaacgc tgaaatcgct tctgctattt ttttcatgtt cttttccttc
     3241 agttgctgac cattcaagta acgggaaact gttcccactg aaacaccagc catcttagcc
     3301 acatcacgga tcgatgccat acattctcac tcctgccagt tacatttgat ttctctttct
     3361 tgttgttcga ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc aatatccata
     3421 taactaaagt cttcagtaga gcaacttcct ctcactatta attatactta aacaagtatt
     3481 tgaaccggtt catttttaat atagcatttt tttaaaagat tgcaacactt ttttaaaacg
     3541 ctttctgaaa gtggcggatt ttttgccttt ttaaaatttt attctaaaat ttactctatt
     3601 tgtgggtgtt ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt ccaatggtat
     3661 aatttcttta tcctatcaaa tgggggaagt attttgaaga aattaatcgt actatttatt
     3721 ttgtctgcgg gacttttgac attgactggc tgtgatttgt ttggcggctc tgaccaatcc
     3781 tcggacgaaa caaccgattc ttctgtctta aaagcggaaa aagcagatac caccaacagt
     3841 gtatatacgc taaaagtacg taaagatatg gatttatccg cgggacagtt gacatttaca
     3901 gataaaaaaa tggaatggac acgcacgtat cagacacaag gaactgattc ttctgaatca
     3961 tctgaagaaa aaacagtgct gacagatatc aagatcacta ccaaagatga cgaatatatt
     4021 atttcaggaa aagaaaataa aaaagaaaca acagtcatat ttacaaaaat cggtaattac
     4081 cgtttgaagg atgaggatgg caacatctac agtctatagt gccgtctgtt ggatcttctt
     4141 ttgtttctcc ccttcttttc tgctatagta aagaagcaag atgattgtaa aggagacgaa
     4201 atggatgatc gatagtcctg attattgtac atgtaataac aacaaaaata tccatacaga
     4261 atttaatgac caaggaagct tgagcatatg cgacaactgt cacaaactag tcaaagattc
     4321 atttaaaaca tttgaatctt atcctgttca aaattatgta aaaagtagaa gaagataaac
     4381 aaatccaagc aaaactcact atggctgatt tttatgaact tcaaactcag cttcttcctt
     4441 tgatcaagct tcatcagtaa aacaaacagt ggatcatttc agtttacaaa tcaaagagta
     4501 aagatttatt tatttcaaat atttgaatat aaaataacca aacgatagaa aggcttattc
     4561 tacatcacgt agtaattttt ctttcccgtc gtttggtttt ttatttttag cctaattctt
     4621 gaccttttgc atgctggtta tccgtaaaat attttccagc aggctttcct tccaattcag
     4681 ctaatttttc ttggatctga cggatcacct tttttgcatc ttctggattt tctacaaaat
     4741 cgaatttatc cccatccact tgtattttag gacatacatc aaaatcttca taccattgat
     4801 tatagcgggt gttcaacgtt tcataatagg tatataacgt ctcatcgctt tctagctgtt
     4861 catagtcacg cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa actttgatat
     4921 ggaccatcaa atcaggccgt ttttttggag caacatcatc taactcgttt agcatcgtat
     4981 ctaataagct atcgtactgc tgtacttcga tatccgttac ccgacctaaa tcagcgttta
     5041 gatggaacaa caggctatct tcatagatgg atcggtctag cacattgtca tcgttcacta
     5101 aagcattttt cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt
     5161 actgctcagg atttgaataa aagagcggca atacttcgtt atcttcgaca ttttcataaa
     5221 atggtttaga atcaatctct tttgcaagca tatccgtcaa actgctctta cctgcaccaa
     5281 tcgttcctga aatcaaaatc actgacatac aacccttcct cttctataag tactcgccta
     5341 cgtaagtttc aacaggcaat gtttcaaaat catattcacg tacagcttgg tctgatttta
     5401 aatacgtatg gagactttgt gtaatcatca agttcgtagg taccgtctct tcttgtacaa
     5461 tgatcatcgg ttttttcaac atagttgcca cacctaattc gtaagccgtt cctggatcga
     5521 ttgtttgatg ctcaaagtca aggatagcaa caacaaattc tgcatttgtc agttcttcca
     5581 tatctttttc gtatacttcc tgcgcccatc cagcgctgaa tagttcataa ttgctttctt
     5641 ggtgatgacg tggagagtag aaacttgtga ctgttttgtt ttcctccaac gctttttcga
     5701 tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac
     5761 tcattatttt gcctccttca ataataactg atttttaata gatactgctc gtaattcatt
     5821 gaccagttcc aatggattgt tggaaaagat tacttgatcc aaccattttt tatccataaa
     5881 atcctctttt gcaaaagttt ctaataattg ttgcaagccg ctgtaataac cgccaatatt
     5941 caatattccg attggcttgt cgggcgtcag accgattgct atccaagaaa gaagttgtga
     6001 gaattcctcc aatgtaccta gacctccagg gaaaacgagg acagcatctc ccttttcaaa
     6061 taataatacc tttctttcat cgatcgtttc tgtaggaata aatgtagtaa catcttttcc
     6121 aggcaattca gtttcaaaaa gcccattcgg atagatacct gtgacttttc cgttattttc
     6181 cagtactgtt ttactaatca ctcccataat cccggagtat gaaccaccgt aaaccagttc
     6241 gatttcttct tgtgccatgt attttcctaa cgtttgcgca ataaatttgt aactttcttt
     6301 attaccaaaa cgggaaccgc aaaaaacagt aagtttcata aaaaaacact ccctaactca
     6361 aaagtctatc ttcattctac acgtgttttt ttaaaataaa agactagatt ttgtagtttt
     6421 ttagtggtat ataggataga cgccactata tatagtattt agaaaaaaat catttttcag
     6481 ccattttata gataaaacaa aaataaataa cgtgttttca tcgatatgaa atgacttact
     6541 tatagaagat actgggattt cgtcctatat gccaataacg tgtattattt taaacaacaa
     6601 atacttgttt cttttattaa cgaaaaaatc attttgttct cgtgttatgt ttcactgata
     6661 aatgatgttt ctctaaataa aaaagccgac gaactaaacg atcaaaattt gatcattttt
     6721 gttcatcagc ttctcctaaa aatctttttg ttaacgataa atagctggat ttgtaaaaat
     6781 atcacttgaa tcgtcactgc tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc
     6841 ctggaactag tgtttgatat tgggctcgaa cgtgtactga tctctagcat gaagaataat
     6901 ttcttttttt actgtataat aactttcttt cctttttgga atagtacagg aaagtttttt
     6961 tgtttccttt ccttcgacat aaggtatacc agaccctttc gacaacgaaa ggttctcatt
     7021 tttccttcat cgtcatttca aggagggtgc cgatgctctg gacagtttcg atcgctccta
     7081 aaccaaaatc tgtatattcg attctttcta actctgattc tttcatacca ttcctgtctc
     7141 aaggtacatc tattgtactt ttttcttgta atccattctg cctctcctaa ctaattgtca
     7201 aatcatccca agatatgtct tcttcctctt tttcttcttg gatgatttct gcattcggat
     7261 ctactggttt tttcaataat aaaatggtta ttcctgtgac aaaagaacca gctaacaatg
     7321 caccaatgaa tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa
     7381 tacggctatc tgcgccccaa aacatgctta atccgccacc tactgtccca ccgactcccg
     7441 ttgctatgac attgcgtgcc aagtcgttca atacgatagg gaaacatccc tctgtgatct
     7501 gacagatccc cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgagtatat
     7561 ttttacgaat gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg
     7621 aagctaaaat caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc
     7681 cttatccaga ataaatggta atccagaaac agccgtgtat ctgcggtaat aagcctaacc
     7741 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgcagggcag
     7801 ctttttcaca acctcttttt ttatttatgt ttcataatca atatggtaag gatgcagctc
     7861 cgtatggtag accgcctccg aatattcaat catttcccca atttgcgtag acgatttccg
     7921 tatccgtttg atgccgatct gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa
     7981 aaatactgcc gagaaatcat cagaaaatga gtcaatagta tatccttttt tcgtcagcaa
     8041 acgatacaaa gattctttgc caaattcttc tctgctgaat tgcatcattt cttgtggaag
     8101 ataatgaaca aaataaatat aaggctgatt gttcaaataa tataagcgct taaaacaaac
     8161 agcttgctca ccgaaaaagg aaaacaaagg atcttccttc tttatatgga ccaattgcaa
     8221 atccagcgtt tctttccgga cttcatatcg ggagttctcc aagatttgcg taaagcttgc
     8281 cgctttggat aatcgattgt aaggacgatt gcttagaacc gtcgtcccac gaccactttt
     8341 ctttgcgaca tattcatctg atgccagcag ttcgattgcc tttcggacag tgattttgct
     8401 gacttgaaac agttcttcta attcactttc tgtcggcaac atcgaaccga ctggatattt
     8461 tcctgagaaa atatcttctt taattttatc tgcgacttcc aagtatagta cacgtgtcct
     8521 tttcataacg tcaccctttg ctttactatt actaaaagaa tagcacataa aaaataaaaa
     8581 aagaacacaa atcacatgtg agaagtgtgt tctttttcat tcagctgctt taattacaag
     8641 tttacgccat ttgaggcaat tacttcttta taccaatcaa aagatttttt ctttgatcga
     8701 ttcagtgtcc catttccttc atcgtctaga tccacataga taaagccgta acgtttactc
     8761 atttctcctg ttgatgcact gacaagatcg atacatcccc aaggcgtata gcccatcaaa
     8821 tctacaccgt ctgctactgc attagccatc gcttcgatat gacgacgtaa ataatcgatc
     8881 cgataatcat caataatcgt gccatcctct tccatatgat caatggctcc aagaccgttt
     8941 tcaacaatga ataatggttt ctggtaacga ttgtataatt cattcaatgc gattctcagc
     9001 ccttctggat cgatctgcca gccccattca cttgcctcaa gaaacggatt ttttactcct
     9061 cccagcaagt tcccttctga agttgctgct tgtggatcag tctcattgat tgccgtagac
     9121 atataataac taaatccgat gtagtccact ggatattctt ttagcaactg gaagtctttt
     9181 tccgtttgct ctaatttttc cggatcgact ccatattttt tatgggtacg cgctgtgtat
     9241 gcagggtatt ctccacgtac ttggacatcc gtgcagaaaa aattgaattc ttggttttgg
     9301 atcattgttg ctgcctgatt gactggattg gcatcgatgc tgtaagttgt tgcatagata
     9361 atcatgcagc cgatttggat atccgaacgc aattcgtggc cgatttttac ggctttgcta
     9421 cttgcaacga attgattatg ccatgcttgg aaaatatttt ggtactctcc tgctccattg
     9481 ctttttacta atccttgact taaagctgga aagtgaaagg ccgaattgat ttcattgaaa
     9541 gtcatccaat atttgacttt actgctgtat ctctctaata ctgtctgagc aaatcgttca
     9601 tagaagtcga tcagttttcg atttttccag ccaccgtatt cttttgccaa atgaagaggc
     9661 atttcgtaat gagaaatcgt gatcacaggt tcaatatcat attttaagca ctcatcaatc
     9721 agctgatcat aaaattctaa tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa
     9781 attcgtgtcc aggcaatcga aaaacgataa catttgaatc ccatttccgc aaacaaggca
     9841 agatcttctt tgaaacgatc gtaatgatcg attcctcgat gattcgggta aatatatttt
     9901 tcttgatcaa tcgtccagtc aaattcttca ctgccgataa tcgcaaagcg ctgcttacct
     9961 ccaggcatag catctgcaac ggatagtcct tttccgtctc gatcataagc cccttctaat
    10021 tgatttggca gcagttgctc caccccataa aaatttttct gaaaaacgtg attgcatcct
    10081 gatttcctcc ttattttaca attgccagaa catcttcgtt tgcaataatc tctttttggt
    10141 ctaattcaag tatatcggca aatgcatcag tattcgtaat gacaactgga gtgattacgc
    10201 tatagccagc tgctctaatt gcttcgatgt caaaacgaat caataaatct cctgctttga
    10261 cgagatcccc ttgtttgact aagatctcaa agccattccc atttaattct actgtatcca
    10321 tcccgatatg aatcaaaatc tccgctccgt cagtcgttgt gatccctaca gcatgtccag
    10381 ttggaaaaat agtcgtgatc tctccgtctg caggtgcaac tagttctcct acttctggat
    10441 caatagcgat accttttccc atcacgccag aagaaaaaac ttggtctggt acttcactca
    10501 atggcagtac tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt
    10561 ttcctgcatt cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg tcaaatccta
    10621 aaatcaatgt acctacgaat gcaagaacaa aagcaattcc tgtagctatt actgctactg
    10681 tcacatttga ctctacacca tccaccgtat taatgaaagt cggaatcgtc aataagctga
    10741 ccaaactgct ggagaaagct tttactccgg aaaatccgat aatagctcca ccaatacctc
    10801 cagaaataca agcggcaatg aatggtttct tcaaaggtaa tgtcacacca tacacagttg
    10861 gttcagtgat tccaaataac gaagtcactg tagaagaaat ccctaatgct cgaagtttcg
    10921 tatcttttgt tcgcaatgct acagctaaag cagccccacc ttgtgccaag atggctggaa
    10981 gaagcattgg catcaataca tcaaacccat attgttcgat attcaaaaac atgatcggta
    11041 cgaatcccca atgcatgccg aacatcacga atacttgcca taaacttccc atgattagac
    11101 ctgctacgat tggactaaat ccataaatcg agttgaagag accgcctaaa gcattaccgg
    11161 caaccgtccc gattggtcct aaagcaagaa atgttaatgg aaccatcact actaaaacga
    11221 caagcggaac taaaatcatt tgcagaaatt gcggaatcac tttttttacg aatggctcta
    11281 atttactttg aacccatacg gctagaatga ttggtatgac agaagaagta taaccagatg
    11341 gtgaaagaat cactgggatc ccaaagaaac tgatagcttc tcccgcacca gcaactgctg
    11401 tgatagctgg atacacaagt gccatagcaa ttgctactgc taaaaactgg ttggctttga
    11461 actttttagc tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca ccatctgctg
    11521 cagcaaacaa tatttgataa gtaccgctat cagctgtcag ccagcccatc accgttgcca
    11581 atgataagaa tcctttcaat accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata
    11641 tactagagat aatatcgatc agacgattca aaatattttt ggactcttct tttccatcct
    11701 ccgaaactgc aagaccttcc tcatccacga tcgattgata aacatctgct acattactgc
    11761 caatcactac ttgatactga ccgccacttt gtacgacttg gatcacgtcc gggtcttctt
    11821 ttaacttttg cgtatctgct aaagattcat cttttaatct gaatcgcaac cgcgtagcac
    11881 aatgtactaa acttttgaca ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa
    11941 ctgcttgatt atccattgtt ttttccttct ttctttttta tttaaaaaat aaaaacccag
    12001 attgaaaaaa acagtcacaa acattcgtga acctattttt tcaatccagg ttttgcctgc
    12061 catacagtaa caatccttat gattggatgc tttttttctc acttactact ctttgaatat
    12121 gaatcgttaa ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat
    12181 actcattgat ccgttcgaca caatgaaacg cttttggata ctttccttga actaatgcgt
    12241 agagaaattc atcttgctcg tcagatcctt cccctttgag ataacgctgg gcaaaaaact
    12301 gcagatgagt aatgatacgc tgataattca aagattcttc atcgaacacg atgccgaaga
    12361 atttactgat gatactcagt atatctctta ccattgtggt agcggtcatc gtcacttgca
    12421 tgtcttcgtt tccttgttgg ctattcacaa aatgaaaggc aataaaaccg gcttcatcct
    12481 cagaaagttc cacacccgtc ttttgtttca cgaacaaaag agcctctttc gctgcttgat
    12541 attctttcgg ataaaatttt tttgtttcaa ataataatgg attaggcagt tggatatttt
    12601 cttttgctcg tgtgatcgca taatgaagat gatcggtcaa tgtcagataa atattaggcg
    12661 aaaattcaac ttttagttta tcttcagcca gttcgactac ttgtttgacc cattcgatct
    12721 cctctgccgg aatatcagcc atcagctgct ggaattggtt tttagcaaaa gaatctctta
    12781 aaacaaattc tttttggacc gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag
    12841 ctagtccttt tcccatacaa acgacttctt ccccgaatcc atttttcgaa atgacgacat
    12901 tgttattcaa aattttatca attttcattt tgcttcgccc tctcaagaaa aataaaaaaa
    12961 cctagaattc gcctactgtt tcttagctga attctggttt tgcctgccgt acagtaacaa
    13021 tccatttctt attcaaaatc atcataccat cgaattttta tttatgcaag ccttttcata
    13081 taaaaaataa aaaaactgga acacaagtct atctagaaaa caaccaaatc agagatgcac
    13141 ctctttgcgt ttctcttttt ttcagcaaat tggtttttcc ttatcgattc atatcctttg
    13201 gctgttttta atctgtaacg acttatgtcc cagtatccta aaatgttttt gtctctagat
    13261 tgaaagcaga tctcatatca tctgtcgcat cttttatcga tgtaacactt tccagttctc
    13321 cttgtacaac tagtcgcgtg acggcgtcca tgtcgccaca agtaattagt gtgacaattt
    13381 cttttccctc gacttcattc agtaattggg tatctgtagg agcaactttt tctttaaaaa
    13441 atgttttgta tgtatacaca ttttctaaat ctgtcaaata gatcttggca cccatctcta
    13501 aattttctag cggtgtaaac agcaattcag ggttcgttgc acgatggctt gctaaggcat
    13561 aatttccttt tcccatttct tgatctggag ataatgttcc tgctccgtaa agtagtgacg
    13621 tattatccaa gcctttgaaa accggtaaat tgattgaaac tgaagggact gcgatactag
    13681 caatcactgg tagatctgtt cctctcattt gcgacctcat gaccccttct gtcgtcattg
    13741 gttctaccgc atcaaagtca aaactgacat ctttatcgtt attctttttc agatcttctt
    13801 ttgttacatt cgctatagca tatttatctc ctgtctctct gacaaaataa tccttgatct
    13861 gttcattgaa aatcaaagct agtcctacta ataacaagag gaacaagagg atattgacta
    13921 gccatctttt tgcttttcct ttttttttca ttttctctcc ctccagactc attttactgt
    13981 ttttcttttg ctctttccaa cagccgaatg acacatttct acaactctgt gtattttttt
    14041 cgtacacctg cgcttttttc tacaggatat ttcaaattat ttttgatcaa tttttcttca
    14101 atgatctcat ctagatccag gtgaaggttg tctgccatca tgtatgaata gatcaataca
    14161 tctgccagtt cttctttgat tcgttctaat tgtgtttctt ttacttcttc cggcgttttc
    14221 cattgaaaaa gttctaacaa ttcagatgct tccaaagaaa tagagatagc caaatctttt
    14281 tcgttatgaa attgtcgcca attacgttca tctctaaact ggttgacttt ttccatacta
    14341 ttcatttgct tgccttcttc caatcacttc ttacttgatt aatcatacca tttttttaca
    14401 taaaaaatag atgatgcttt taaaaaaact cctgttttct tttatttaaa ataaatgtca
    14461 tagaaaatca ataatttagg tagccaccat gtgtatgcag tagcgtgtct tcttcccttg
    14521 aggctttttg tttttcgata cgttcttctt cccaaacaga caacgctact agaatctctc
    14581 ttaatgattt tccttcttcg gtgagcgagt actctacttt aggcgggact tggttatagg
    14641 cttttcgatg gatactttca tcttgttcta actctcttaa ctgctgggtc agcatttttt
    14701 ttgtgatcgt agggatttgc ttgcttctcc acgcctaaaa taagcgatat tgctcttccc
    14761 tttatctggt gtaagcaaag cagttagcat tttgacgatc gtgggttccg cttttcactt
    14821 cttatctact tagtatcatg aagtatactt tcgtactaat actaagtagc ggaagtgtca
    14881 actactttgt ttaactacat agcaaaagaa acagaagggt gacaacggtc atcacgcttc
    14941 tgtttctttt gaatatgctg tgttgttgtc tctatctccg tgtagacttt cctcaaactt
    15001 atcctttgac agtaagtcag taggctgatt tgctctttct agatgccaga tacatttcta
    15061 caaaaaaata gtgattctgt tttgaaaatt cgatttttag ttctgcattt tcgtaaacat
    15121 ccaacatttt tttcgcactg tatagaccta acccgccatt ttttttagag gtttttcctc
    15181 ttttgaccat ctccgatagc gtcaaattcg ttggatttgc cttgttcgct atactaaaac
    15241 gtgatccctc tttttcttcc agcagattga tcgtaatcgg actctgatcg gctacacgat
    15301 gatcgaatgc gttagaaata aaaatagata aacagcgaat aaaaacaacc agatcaatcg
    15361 ggattgtttt cacttcgaca ggaataacta cttggatcgg gattttctcc gattctgctt
    15421 tttctagaaa ttttaaaaag acacccctga cagctgggga tacaatattt gttagctgtt
    15481 gtatttgcgg tttttcaaaa atagctgcag aatgatcttc taatatttct aaaaattcca
    15541 acgtttcttt tttattttcc tgaagagtaa tttttaatga aaacagtaga cttttataat
    15601 catgtttgaa ttcctctaga tgatggtaat attcttcatt tatttgatac agttgaagcg
    15661 attcctgatg ttgtctttct tttacttgga aactgatcaa cataatggaa aaaaacaaag
    15721 aaaaaaaact gatgactatc gacaaaagag atagaagcag ataattagat gagcggaagt
    15781 gaacacttcc caataccaag taaatcacgc cccagcttaa taaaatacaa atacctccaa
    15841 aatagtgttc agttggataa gtaaaaagca agttgagaaa tctcgtctgt ttgtctatac
    15901 tttttactaa tagatagaaa ataaatgcga taagtagtgc aaaaaaactt gctgttccaa
    15961 gatgtctctt tcctaaataa tccatcagat caaaaaccag aaaaaagctg agtccagtga
    16021 tagcaaaaaa aagattactg atcaaacagc tgataaacag gtttttcgta gtaaaataaa
    16081 aaagaaacat caataataag ccaactaggt aaggaaagct cactttaggg ataggaagta
    16141 aaagtgtcac caccatcaaa gctatacaac caaagctgtt ttttactagc agttgtttcg
    16201 aaaagaataa atagatacct aaatctaaaa tcaaagtaaa ggtcaaataa agtgtcactg
    16261 ttgcgaccat cttattcctc cattgttttg tttcaaaatg tacacatatt tttttaatag
    16321 gagcctgcat atgaaaaagt attttatcat agaagataac cagtttcaca gcgaatggat
    16381 catcagagaa ttggtcaatc atcatatcga gaatatcgtg cagctcactt ctatctctca
    16441 aatcaatcag ttgatccagt tgggcactat tgataatcac gatgttttct ttatcgatat
    16501 cgacttgaag caatcactca caggtattga tgtcgccgaa caaatacgta aaaaaaatgc
    16561 acatagtaat attgtttttt tcaccgctta tacgaatttt gcagtagaga tcatcaatcg
    16621 gaaaatcctt ccgattggat atatcagaaa agacaggaat ttatctgaac aaattcgtca
    16681 agtgctagaa cagatcaatt ggaaagaaca gcagatcgta aatgtcgata aaatctttat
    16741 tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa attttatata ttgaaagtat
    16801 caaaggtttg aaaaaccaag ttatggtcaa aacaactaca gaagataaat tagtgaatac
    16861 ttctttaaaa caattgaaga cagagttaga aaagtatgct tactttttac ttttaaaatc
    16921 ttactgtatc aacttatcgc aattgcagaa aattgattct gctcattatg tgttagaatt
    16981 tttcaacggt gactccttac tcgttggtcg aaagatcttt gaaaagacaa aggaacgttt
    17041 ccatgacttc caaaaaactg cttcgagttg atgaataaga gctgagacgt aagcatagca
    17101 gatttcaggg agtgggcctt gaatcggctc cactccctga aatcgttgat atgattttac
    17161 gcttcctctt ctgtaggagc atctgttaac gaccatgtga atgttgaaac gtagttgtct
    17221 acagcataat tggatacagg aacaaataat tccactgatt gtctcccttg ttccacattc
    17281 tctcctaatg aataaatcca tgtgccaatc ccttcatttg ctcctgcagt tactagatct
    17341 actgcctgat ctgctgggat ttcgattcct tcagcgccag ttccagaaac ggtagatggc
    17401 attccactgc tattagttgc gctatttcga atcacggcgt ttttgaattt cacctttgaa
    17461 ccagttagtt tattcgtatc agatgttgta ccaataaatt ctgttcgttt gactgataat
    17521 ttccaaccgg cataatttcc gcttttatcc gttacttgta cgaaattctg tacttgtgtt
    17581 tgtgctccag tactgtccgt tccgctttgt aatgcagcat atactgtttt tgctgtacct
    17641 aacttgattg ttccaaaatc aagatctgga gcttggtcaa tcgtcagtaa tccggatgtt
    17701 ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat catttggatc aacaggagga
    17761 actggaacaa cgtcttcagg atttgcagca tctggtttgg tcacagaaat cgttccttct
    17821 gtagtggctg agctcttatt ctcagcaaaa acactttgtg tacataataa tagacttcca
    17881 gctagaacgg tacttaatgc aattgttttt ttcatttctt tttccttctt tcttttaaaa
    17941 taaataatcc agcaaataag ccaataagaa tgactccaac aagtatcagc catcgccagt
    18001 caaatgtttc tggctgattt tttaaatcaa tatccttttc attcaattgg tccgctgcat
    18061 catcagttat ttcaaaatcg gtatggagat tccagcgttt ttctttccaa gcacccttca
    18121 caaccgcttg ataatgtccc ggagctaatt tttttccttc taatgggaca agtaaatcaa
    18181 aatttgtatt gggtgctaca tctatttttt ctttttttat tgaaaggtaa ggctcttttg
    18241 tttctttttt gtagatttca accgatgtag tcaagccgaa catataagaa gcatttggat
    18301 tttgaagatt cgccttaatc acatttcttc catcgctttg atccgcataa gcttttttta
    18361 atttcaaatc tggggaagac tctttttcgc tatttcaaat caagattgct ttcacttgag
    18421 aaaactgatt gacgacacca actgtacttt tgttagaaac gttctctttt ttttcttgaa
    18481 acgtgatccc tcctgcgacc acaccagcca gctctttttc tggcgctttc agtgaaagtg
    18541 tctcagtgac cgcactttga ggaggaacag ttatttctcc cactgttttt gctaagtttt
    18601 tcatatcata acttaatgtt tcatcaaatt tactttttga ttctccatat tcgatcacac
    18661 cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac tgttactgat ttatctgttc
    18721 ggttaaagac cgtgtagggg atttcctgtg ttttcccagg ctccatcctt acatttatat
    18781 atgttttgtt cttatcaatc tgattagccg gtaattcaaa atctaccgca aaattgaatt
    18841 cggaggcatg gacaggtgtc tgccaaagga tgaatccaag gagaaggatc ataagtaaag
    18901 agcagttgtt tcgatgactt ttattccata ttcgattcat caatcatcct ccttgatctt
    18961 tattctgtcg gcgcatctgc taatgtccat gttatctctg ctgagtaatt accagctatc
    19021 tgattttctt tggttaagac aagtttaaac gcccgaccgt tatttaaata ttctgaataa
    19081 tctgcttgca cattcgttga attttgtcct ctgacaattt ccagttcttc ttcattcact
    19141 aattgatact cctctccttt cttgaaatat aggacatcct gcatagaaaa tcctgcatca
    19201 tttgtgaaag gctgagataa ttttatcgtt aagatccacg gatttttcgt ttctcgctga
    19261 tcataaatcc ataatttttc ctgtatatcc atttggccaa atgcttcttg tgtcaaacca
    19321 gttgctttga tctgaccaaa atcgagtttg gaaggggcaa gaaatgagag accaccttct
    19381 actacgatct tcgtagcagg tgcaaaaggc ggggcatcat gataaacaac tggcgtatca
    19441 cttggattgt gattcccaat ttcatcgttg gtacttggct tgttctgcac acccttagca
    19501 gtagcttctc ctgcttggtc gtttaataca atttctaata catctcctgc aaaaagttga
    19561 ttttctggga tagaaagaac aaattcccct gtatcatcaa tagttgtttc tgacaaaaca
    19621 gcttgatttt ttttcaatga aaccaaggct cctgcttcac ctgtacctgt gatcgtgcga
    19681 gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt caggaggaat cacatcgact
    19741 gtcgttttgg cagagtccag ttctgtcact tcttggccac ctcttacttt tttagcagca
    19801 acaacttgca cttgatcctc cgtttttaat ctctcatcat tgggtacttg aacttcgaac
    19861 aagccgcctt gttcgccttc tccccatggg ttatatcctg ccccttcatt ttcatctgtt
    19921 tgtccttgcg tgagagcagt aatttctaca gatgatttcg tcgtatctgc gtaagtaata
    19981 cgtaaagtca cttctacttc gcctgtccaa gcatctcttg gctttgcctc gacaccttcg
    20041 ggaacaacga catgcccata gattttttta tctgcattcg ttggcactct taaggattca
    20101 atcatcggga gctgattatt ggcactgatt cgattataat ttttcattcc ggcacctgtc
    20161 tgagcaaatt catttgttaa acctgcacta gggcttgata cgaaattgcc taagtcggtc
    20221 ccattgatcg ataagtctgt catatcagaa aaataatccg ggtctttttc aaagttatcg
    20281 aatgttgcgc ctgtttttcg ccataaagcg atttctgaat gggcgatctt taaagaagaa
    20341 tctccatttc cttggaatac ccagccgcct atgccattgg tctcgcttcc aatccgtttg
    20401 ttttggaaat caaaataggt aggagattct aagtccacat tcaacacatc cccactgatg
    20461 atgccggtct tatcttcccc tgttttcccg accatgacta aataagtttt ttcacctgcc
    20521 gtaaaattta aatcaatatc tccagttgta tctacagttg ctccggaatc tgcgcgtata
    20581 tcgatatttg atttttctcc agtgactgta aaatcagctg aacctggact ttctagaaaa
    20641 ccagaagcgt cataaaattg gatcgcttga tttccttgat tgagtctgtt cacgtaagat
    20701 ttgccatcac ctgtatttct taccctcaat gagcctccat tggttactgt cagcttttgc
    20761 gtaccattct caaaacgaac tgcgctggaa cgcccactct gcttatcgat gtcgataact
    20821 gatccggctc cttctacgat caaagaaagg ccttttccta caaaacgcat cccattgttc
    20881 ccgggttgat cgtcatctcc agccattttg accgagattt gtccaccact tgatacatgg
    20941 atttgactag aatctccttg caagcgaaat ccgcccgtag ttgctgtatt gatggtgatt
    21001 ttcccaccat tcgatacagt gactttcgat cctgaacctc tcatttggat gattccgcga
    21061 ttgttgtcat tggtaatatt gatctttgta tttaccgaaa attcgcttcc cgaaccactg
    21121 acttcaaaca tgacattacc aggtagcgta tcgactgcct ctccttcatc gctaacgcca
    21181 tctgcccgat cataatcaag agagaccttt gaaccgctta agatatggat tcctggcgct
    21241 gtttttccac taccagtctc atctcctgaa tctagaaaac cgtagaattt ccctagatac
    21301 ggtccataaa gttgactacc attgatcaca ttcaattgat agcttctaca aaaataggct
    21361 ttacccggag cggcattcag tccttccttc actggttgga catccaacac accggaaaca
    21421 ccatctaatg tgacagttgc acgaggagcg taaatcagtc ctaatttgga cccatcatac
    21481 aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat gatatctgct tccccaaaaa
    21541 tcggtcatat aagcaatgat aatggcattt ccatcgatat ttggtgtcaa tccacctgga
    21601 tgtgcagctg ttcctttaaa attctggaac tcgatcgact gaggtatctc attgatataa
    21661 atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat caataacgat atctcgtttg
    21721 atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc ggatcttagt tactgattcg
    21781 tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt cgctgtaccc atccgctgaa
    21841 acagctcttg ccagtgccgc ttcactgcta tcagacgttt gttcagctac ttctgaggtc
    21901 tcacttgtct tattttcttc ttgcctttta ttagtagaag cctcactttg agattctgta
    21961 gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt caaaactttg attctctgat
    22021 tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg atgtcaccat caagagaggg
    22081 aagacaaaca aaatcgacgc aatcagcttt tttacacatt gattcattct ccaccatcct
    22141 ctctgacgtt ttgacaattt tattttatcg ttttaatcta tttccaattg tactttcata
    22201 tttaaaatat gtatttttat ggaaaaattt ttctattagg tgtaaatttc ttttttgtac
    22261 aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta tcccgaacaa gcggtgttct
    22321 cccgccaact cctcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggcaa
    22381 tactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc
    22441 ttcctcatca gctaatgaca caactaattg ctcacaagta aggtaggcta gatttttcgt
    22501 agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga
    22561 atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt
    22621 ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag gtactgctct caatttcttc
    22681 cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat
    22741 tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt
    22801 gattgattca gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt
    22861 tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc
    22921 tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa
    22981 atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc
    23041 gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc
    23101 atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc
    23161 tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa
    23221 taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca
    23281 taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga
    23341 caagataaaa catccatact atgaaactga ggaaaattaa aacaatgata taggaaataa
    23401 aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac
    23461 acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag
    23521 cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac
    23581 ggccttgaat aataaatgcg acaatccccg ctataattag tgtgcctaac accaaaacgg
    23641 aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg
    23701 tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc
    23761 actccattta tctacttcta cgtgcatttt ttgatattgt tttctttatt taagattata
    23821 cagaaaaata tctagggtcg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac
    23881 taaatcaagt agtaattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac
    23941 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
    24001 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
    24061 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
    24121 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
    24181 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
    24241 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
    24301 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
    24361 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
    24421 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
    24481 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
    24541 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
    24601 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattgag
    24661 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
    24721 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
    24781 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
    24841 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
    24901 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
    24961 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
    25021 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
    25081 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
    25141 agaacaaatg ttatcgattt tgtttttctt cttccgctgc tgggatattc aaatcagaca
    25201 cttttatgtt cgattttcca gacatttttt tgttgttttt attatgatat atttttatac
    25261 aactaaaaat gaatatatta aaactaccaa aaataacttt taacgctgtt tcattatatg
    25321 ttttttctat tttatataaa cttttggata cttaatacca accggttgga ttgtaccaac
    25381 atgagaatgt gtcagttcta tttcctgtcg cacttgcata ggcccatact ccttttgttg
    25441 catttgctga tcgaatagag tttccaactt tcgcttttgc accgtgacga ttatagacac
    25501 gactatagtg gttagaatga cctgtttttc tcgtacaagt tgtatcacta taataaaccc
    25561 atcttccaga gctatgatta attgctgttc tagaaatttc ccggtctact ggtaaatttg
    25621 acttaataat attaccttta gcatcaaaat gaatctctcc tggctttaaa gatttgtaat
    25681 agatttcacc gtgaactgaa tttgcttcca cttcagatga catagcgttt cctccaacaa
    25741 aacctatccc tagtgcacaa ataataactg cttttttcat aaaacgacat cctttccttt
    25801 ttgattttta aagcatgatc ttcttgtttc ctattttggt cagatatgga tcatgcgtga
    25861 ctacgataac taacgtacct tgggaagctt gttgcgataa gatttcaact actttatcct
    25921 tatttctttt atccaatgac ccagtcggtt catcggctaa aatgattttg ctattcttca
    25981 ataagactct cgcacaggcg attctttgct tctcaccacc agataatgtg tagataggcg
    26041 tacttagctt cttgtttata tttacttttt tcaatgcctc atactttgct gttcgcttat
    26101 ctgaaacatt taaattcgaa aacttcaatc caatatctaa attaaaatcg attgtttcat
    26161 attcaatcaa cccaaaatct tgaaaaagaa aagatatggt atttcttttt atcaagcgct
    26221 tctctctccc agttaattgt ttcgtctctt gatcgaacaa atagaattgt cctgaactga
    26281 actgatcatt caatcctaat atattcaata acgtactttt acctgctcca ctttctccaa
    26341 caacagtcac aagatcccca gcatccgcag tgaaacgatc caaatgaaga atcgttttgt
    26401 ttcgaatgat ttttttcgca ccttttatca ctatcaacgg ctccaattgt taacctccat
    26461 tcatcagcaa taactgactt ttcttttctg tagacaaaat aaaaacaact gaaagaataa
    26521 aatcaatgaa gctaaagaaa aaaatattaa aaagatattt aggctctctt tctataagaa
    26581 aaaggaagat tacacttgtc gttccccatt tgatgaggtt catcatgaag aaatctttat
    26641 acgtttcaaa gagcgaataa ccacttgaac gaaagatagc gattttccga caattgtaga
    26701 caaaatatat gcataacatg acatagctca acaagaagaa agaaaagaaa gtgagtaaaa
    26761 atatctgtat caagtaatga aatgatctgc ttaacacttt gacagctatc tcttcagtct
    26821 gagtatacgg aaatgataac tgcaagttat cacgtaactg actttcttca atcagctcaa
    26881 ataacctttc tttcgacaaa gagcctatct tgatctttaa cggtggatag atttctccgc
    26941 tgagaatatt tctttccaaa tacatactgt ttttcaatgt cagtatttca acaaccggat
    27001 aatgttcgat ccaggggata gaaggaataa atgtataaat gggttgattg ttttttataa
    27061 atagaaattt cggttctttt tcactgattt cttgataata ttctgttatt tgcgccaggt
    27121 cattttttaa atctgtatgt ctatatcgtt cagggatcaa tataacaggg tgcgtttctt
    27181 tttcggttat ttggatcaac cttccagaga catcaatcaa aggaaatttc ttaagataat
    27241 tcagatttat ttttatccct ctgccaaact gttcattttc ttcattcaag taatctgata
    27301 cattgactaa tatacttccg tttttattca aagtttcata taattcctga tctgctgctg
    27361 atgcaaaacg attggaataa ataaaatcca cgtggttttt cccgatggtt actggataaa
    27421 atacatgata gtcctcacca gcgatttctt ttggtggttc atgggaaaca gctaactgtg
    27481 aagaagaaaa aacgatagtt gctaattcga cattggcaac ggtcaaaaga agagaaaaca
    27541 acaatccaaa tgtaaaaaga atgtattgga tatagttgaa cgcttctctt aacgaagcga
    27601 ttttttcaac taataaaaga gaaatcaaag agataccata tattccgctg aacaagaaaa
    27661 cgtacatctg gaacaaataa agaaattgct ccttataata cataaaaata aaaccaccaa
    27721 tgattcctgc aattatgaat gtaataatga ttttcttttt ccccaataaa caccacaata
    27781 cttgatgtaa tgaaaagtca ttattcaaca ataaactgat ttctttattc atcaataaga
    27841 aaaaggatga taacaaaagg aataaaagaa tcaatccaat agttaaatac actcgataat
    27901 tatatacgtc tgtatttgaa ataaccgaat acgaatagct attttcaaag tcttcaaaaa
    27961 tattagtggc ataatcagtg tgaaatgctt gattgaatcg atcagtgaat agtgaaatga
    28021 accgttcata ggcttctttt tctctcgtca tcaagaaaaa ttcaccttca aaatctttta
    28081 ttttgatcga ttcttcaatg gacgttacgc gaaaggaggt attagaatag ggtaccttct
    28141 cgtgagaaga tttgacatcc ccttgataat acaatgtcat ttcaagcttt ggctgtatct
    28201 tgaagttcca agatgtatct acttcattgg attcatttac tgtttttttc atataagaaa
    28261 gctcagtttc tgatgagaca ttttcaaata ccatgattag ctgctcatat tcttcttgcc
    28321 caataaaatg ttgtggcaat cgaatcggag tataagtatc tgtttgatgg aaaaattcat
    28381 ttgtccgata gagtacctgt ttatttatac taaccaaggc aaaaccactc atgataccgg
    28441 tcactatgat tgtgagtaaa actgtgaact tttttttgat tatcaccact ctcccttact
    28501 taaatgattc tttgataaaa tcataccgtt tttcatcaaa aaaacgtatc tatcaagtgt
    28561 ataggtcatg tagaataaat ctatatactg tagaatcgaa aggagaagaa gatgaacacc
    28621 ggagaaaaaa tacgaactta ccgcagaaat tgtaagctta cgcaaaaaga attaggcgaa
    28681 aaaattggtg tttccgataa aacgatttct agttgggaaa attctcgtac aatgccagac
    28741 cttgaaatgc tgtcactttt acatcatact ttaggtctgc cgattgattt tccttctatt
    28801 gaatcagcta ccgaatcaat agaaccaaca ttgccagatg aacctcttgt taacaagaaa
    28861 tcttattcat caaaacaacc cttctcactc aaaaagacac tatctttctt tctgctcgtc
    28921 ttttgtttgg tctatctttt tcttatcaat tatcaataca aaaatgaaac gatcgatcga
    28981 ttcaatcctt ttttgaaacc acaaatcggc tataccacac tccctgtagg atacgtggac
    29041 aattccatcg aacgttgggt atgtaatgat atctttggcc atggtacatg gctgtctttt
    29101 cacgctggag aggtagaaaa agagaaaaac catgctgtaa tcaaacacaa aggaaaacac
    29161 gtcatgaacg ttcgcctgct cacttgggaa gccattccct cagattacca agaaatcctc
    29221 agccaagact atgacgacgc cttaaaaaga agccctcagg agccagatag gcattaagta
    29281 ggagatgaac gtagagatag aaaaaatcaa tgcacatgat cttatccaaa aaacaggggt
    29341 acaaattaat tgaaaaagcc ttgagatgaa agttcctaaa taaaaaagtt cagaatgata
    29401 ggtatttctg gcttatagta taatatttac gccacagcct cccaatgatt ctttttggaa
    29461 tcataatcaa ggatggacat tcacaaaaat attttgctca ttcgatccga atagattaac
    29521 gaccaatcta gtaagtaata tagattggta cgtagtcaag ctgcacataa tttagatcaa
    29581 ttttagttct tttttatatt tctatgatct gctcatttac ttttatatga tggcttacag
    29641 taacataaat aaatatgata atcgctggta gtttatccaa gagtactcaa taaaatacaa
    29701 aatggattgt tcaagcgcct atcataatga taaaatgaaa caaataaacg atacaaggag
    29761 gaagagactt tgagtaatgg cagaacaatt ttttcattgt aaaaattgtt cagttgttaa
    29821 tctatgggat ttcggtagca tgggcatttt tttatagagg aatcacacca acctggccgt
    29881 tttatctctt gttagtggga atattagtag gaaattttgc ccctcttcac tatcgtacga
    29941 acttttttcc aactgattct ggcctttttc ttaaaacaaa gacgcgaaaa ttagaaaaca
    30001 ttattgaaat gacaattacc tgtataatca ttttcttagt tatattccta aacgtcttct
    30061 aataagaatc tcttaaaatt agcttgttac agttagttac ctagtttata taacacagtt
    30121 atcttatctt ccttatcaac atcttttgcc aactctttgt cttaaattcg aaacatataa
    30181 gcagagctac gaacaccaag actgtttcgg ctctgttttt tgcatcaaat tcactcttat
    30241 atagctgata atctcggctc tttaaaagaa attattttcg cttcatttta ttataaaaag
    30301 gctaggaacc ttgatgttat caggttccta gcctttattt aagtattacc ccacagaccc
    30361 ttccatatag atttttaaat tacacactag tttaaactaa aacaaacaag gaaaaactta
    30421 ccatttaatc aaaaaattag ctcatatgaa tatattagaa tgtaaattag actggtcaaa
    30481 atctggtcaa aaaagaggac gaatacaggt agtcctcttt tttgttattt tgatattcta
    30541 aatcattttt tattggatta ttcccttgtt cgatatagac ccaaattgat ctatcaataa
    30601 ttatcaaact gagtcatata gagacaaaaa tttcaaaaat gacctgttaa atggtcatcg
    30661 aatatcattt aaaatcaata cgacggagtc agacacggag tcaaaacaag cccgctgaga
    30721 gtaaaaaata tacagtccca taggtaaagt gccacaggac tgtatatttt tttatttgag
    30781 attacctgaa atgattccat ttaattttta tctcattttt tcctactagt tagtatcaaa
    30841 tactctccta ttttctcaat aaactatttt gggttacatg gtatctgaaa cagctaaaac
    30901 cttgtcatta tagcaaaaaa tcactttatc tccgtttcat ttcagataca tttcacttaa
    30961 gcggattcca aaaaaacaaa agaaaaagga acccctagtt agcctatgct ttttattggt
    31021 tcctctcaaa acacggtcat ataacaacgg ggtggggtaa atgaatgaaa aaggtggagg
    31081 aaacattatt caatagctgt cttaaatact tcgttataaa atgtgaacaa ataaaagaca
    31141 tctatcccta ttttctacac ttccacttga ctaccatctt tgaatcgtac aattttctta
    31201 actcttctcg tttacctcaa ttcattctca agtggaatca actcatcatc taacatttta
    31261 cgccatgctc gcttcatttc ttgtgggtat ttaaatggct tcgcttctgg atgtttggct
    31321 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
    31381 cctggtagat attgcagatt agaagcatgt aattctttct cagaaacaaa ccactcattg
    31441 gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat agcgacgttt aatgacaaag
    31501 atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg tcggtaaact
    31561 tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc ttctcgtgca
    31621 tctgaactat tcgcagcata agttcctagt aattgatcaa tataagttga atcaatatca
    31681 tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc tccttctttt
    31741 tctggttctt cctctattaa tgaacccttc acggtctcgt acacgccaat ttgttcttcc
    31801 aaaagttgaa tttgattgtt caaagttgga gatttcacca tgtcatcatt gtaatcgtca
    31861 taagtcacta acgattcata agcacgattc aattcttgaa atgctttgac gtactcgata
    31921 cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc ttttaactta
    31981 ccatatgctt gtttgaatct ttttttggat tcctgataac ttggataaat caaacctgat
    32041 tcttcttttg cttttgaata cagttttgtc gcatcctcga cattcttctc cattgtcgct
    32101 ggcttacgaa acgtcacaat caatcctttt gtcttatcag gatacgtacg attcgtacgt
    32161 gaaaaagctt ggatcaatcc tgcatactct agattacgat ccacaaatag ggtttgaatc
    32221 gtaggggcgt caaaacctgt taatagacga tccactacaa tcgctaaatc gatttgtttc
    32281 ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc tccgttataa
    32341 cgctcaatgt ctgctaatga ccaggaagta tcataatact gattatattc ctcaataatt
    32401 ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa tgaataggta
    32461 atagcgattc gtggaaaatc aggatcctcc atcgttcgtc cttcacgaat gagctgattg
    32521 ggaaactctt ttgttaacca atcaggctct ttcgtcatct ctttgatggc ccgataatac
    32581 tttttggcca tgtcaatgga actcgtggtt aagattgcag atttcgttgg cctgccattt
    32641 cgaaaatcaa attttgtata ggcattatct ggacggaaca ttttatgaat gactttttgg
    32701 atatgttcat ctgcttcaaa aacagctggg tccaaataac tttctttttt gacaggttcc
    32761 atttgatcaa tcatacgatt gatctgttct gatgaatact ctgcatatgt ttctacttct
    32821 tttaatttcc gatagatttt attctctagt gaagtcggct ccacggtgtt ttcatgctct
    32881 acttgaaatc ctaagacaga gccatcttct agcgcattct taatggtata ggtatgcaag
    32941 acctctccat actgatcatg ggttgtacgt gccaattgcc ctttggcttg tttccgattc
    33001 tcaggaaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa catttttttg
    33061 atttcttcca tattttcagc acttaacgct cgatgacact catctactac aaacacgata
    33121 tgttggccca ttaatttttg gaagcgattg gttccttttt tctcttcttg tttctttgca
    33181 tatttcaacg ccgcatctag tttttgacgg gttgtaatga ttacaacatt tgcattggca
    33241 tctgccaaca gggtttcact taattctttg gcacttcccg ttccaacgat caagctattc
    33301 gctttggcgt tcccggaaga aatcccagta ttaaattccg aagcaaattt tgtaaactct
    33361 gtggtggttt gattatctaa atctttgcgg tccactaaca tgatcgttcg atcgatcccc
    33421 gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc tgaaccagtc
    33481 gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc ttgaatcgca
    33541 tgtacttgat aaggatgtaa caccatgagg gttttattgt cttgatcttc actgacgatt
    33601 gtataatccg caatcaaacg atgagcatcc gggatattta acacttgttt acaaaactca
    33661 tataagtttt ctactttttc attgtctttc gttcgccaac taaataaaaa cttagggtgc
    33721 aagtctttcg gtaaggcatt tgcaaaatac cgagtggttt gttcattgga aaccacaaac
    33781 aactgtaagg tcgaaaagat attgttccga aacatacctt cttctgcata ttttttgatt
    33841 tggttaaatg cctgataaac accatctttt gcagttactt gtttcagctc gatttggaca
    33901 atcggtaatc cattgattaa taaggtgacg tcaaaacgtc gatcccgtgc ttctatgtta
    33961 cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc accaatgtct
    34021 tgattggaat aaaggataag tgagacagaa cccaattgcg aatcttcccg ttcaatcgtg
    34081 atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg ggttttcgtt
    34141 cgcagcagta attccgtttt gatcgtttca aattctttat cggtcaaagg aaaatcattc
    34201 agttctgctt gattatttga gataattttt tggcgtaaat tgacccaaag gtcttcttct
    34261 gattttagat ccggacgata cgtccactga ttatgccctt ctccaagtac acgaatcaac
    34321 tgttcttcga cttctgcttc ctcacggtga ctaattttca ttcttcttcc ctcctagaca
    34381 aacatctttt gtaagaagct ttttttcgtt tcttttagta aatctaactt acgttgatga
    34441 agagcgatcg tatcatctag ctgtttgaag aaagtaccga ttttttgttg ttcatccttt
    34501 acaggaacct taacactgat attctcaata tcagaactgt tataagaagg caatgcccct
    34561 acttgggagt atcttggcaa atttatagtt tcaaacaata ttttaccaaa atagatatcc
    34621 caagtattat taaataaata agccatggta ttgttatcta ttaagaaagg gcttgtgacc
    34681 aaacgttttc tattaagcat tattgctgcg ccaactttag caaatataat cgctggcaca
    34741 tcttttattg gcttccattt tttacgctca atttgctcat tagatacata atgatttgca
    34801 tttctcattt catactcatt accaatgtta ttcatgtcgg aaactttaaa aaatggagta
    34861 ccttcagaac caccttgctc ggcatcagga aatccaattc ccgattgagt tttacctaat
    34921 tctcccaact tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat ttctggcact
    34981 tttgctccgt tttcagggaa cattttttgt aagaatcctt ttttcgtttc cttcagcaaa
    35041 tctaacttac gctgatgaag agcgatcgtg tcgtcaagtt gtttgaagaa ggtgccgatt
    35101 ttttgttgtt cttcctcact aggaaacatt gctactatat ttttaaactt ttttagagac
    35161 agaacatgtt gacctgttcc ctctgcttga atagataatt gtcgttggat tccattgtta
    35221 atacgtctta agaagaagtc ggaatcagca gattttaaag taaatacagg ataaaaatag
    35281 cttataatcc ctctatcaat aatgtcatta cggttaaaca caaacacatc attatcactt
    35341 cgactacgga acgtgaaata tcctctaggt agtacaaagt aaccaatatt attttctgta
    35401 gttacttgcc gatttgcaaa atagtcttct tgcaaaacta tccctttttg ttgagatgaa
    35461 gttagcatag gaaattgatt ttgtacactt gtcttttcaa cataatctac aacgaagtcc
    35521 cccaacttcc tctgttccca atcatctgta aatcctggga aacggatttc tggttgtgta
    35581 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
    35641 tgtttgcagg gaagagatag cttctaaaag ttttttttct agcacttgct gttcttgtcg
    35701 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
    35761 atctacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
    35821 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
    35881 gttttcttcc gatagtttat tttgattttt ccctttaaca aattcacgac tagcatcaat
    35941 gaataataca tctcgagttt gacgattctt cttcaacaca atcaccgtcg ttggaatcga
    36001 tgtcccaaag aacaagttcg caggcatacc gatcacagca taaatactgc catcttctag
    36061 taatttttga cggatgaccc cttctgcagc gccacggaat aaaactccgt gtggtaagac
    36121 gatggccatg gtacctgttt ccttcaaatg atagaaccca tgaagaagaa acgcaaaatc
    36181 tgctttggat tttggcgcta atttcccata acgattaaac cgagaatcat ctaagaaagt
    36241 cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat caaatgtgta
    36301 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
    36361 ggcgtcgaca ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
    36421 ttcttgccca tgatatttaa cgttatctgg gtgtgttaag tagttacgta cgtttaacat
    36481 caatgaacct gaacccatcg ttggatcaaa aacactaaag aatcgtcgtt ccttttgatc
    36541 gagcgttacg atttgcgcca tcatatcaga aaccatatga ggtgtgtaaa attctccggc
    36601 tttcttccct gcttctgaag caaattgact gatcagaaac tcatacgcat ccccgatcac
    36661 gtccccatcg tgttctaaaa catctacatc attcaatttc ttgatgactt cggtaatcgt
    36721 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
    36781 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
    36841 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtgc
    36901 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
    36961 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
    37021 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
    37081 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
    37141 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
    37201 taaagttgct atataccctc ttattttagc ataccccctt ttataaaaag caaaaaaaaa
    37261 aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt tgtttgatat
    37321 aacttagact gatcgttaac ttatttttta tatttctaca tagttatctt tagaaccatt
    37381 tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta ctgtctagta
    37441 gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct gaaacagtct
    37501 aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag atacaaataa
    37561 atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct aagctactgg
    37621 agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa aagatggaag
    37681 aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg tccgttctta
    37741 aataccacag aataatcttt tttcccattt tcaagtaacc gaagtggaat gtctttacca
    37801 acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc tgataaagat
    37861 tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc ttcatttaca
    37921 agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat tctttcatct
    37981 atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat tctttcatcc
    38041 atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct cttcacaccc
    38101 aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct tttactaata
    38161 aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact atcatactca
    38221 aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata atcctttgac
    38281 tctacttgag accttcctat acggaataca gctttactgt ctgatatctc tataccagca
    38341 tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt agtttcttgt
    38401 gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact gttagaagaa
    38461 ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt gtgcagccga
    38521 tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca gttcgtcctg
    38581 atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg ccgcccgata
    38641 ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct ccggcaactc
    38701 ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag
    38761 catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag ccaagatatc
    38821 ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac tttgggcatt
    38881 tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat tcattttttt
    38941 gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat acttttgaat
    39001 ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt
    39061 caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa acttggcgtt
    39121 ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag cctcaaacag
    39181 tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata ctttttggat
    39241 tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt tccccatcaa
    39301 aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta acgttttaat
    39361 tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat tactttttga
    39421 taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc atcggttcgg
    39481 tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac aacatcaatc
    39541 gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc ttgaatagcc
    39601 agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct atcttcctca
    39661 actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc atagcgatcg
    39721 tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt
    39781 ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa catgatctgt
    39841 tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc gatggtggca
    39901 gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat tgcctgatcc
    39961 tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa caaataattg
    40021 tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc ccaattatgg
    40081 gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa attgattgct
    40141 ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct agcttgagct
    40201 aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc tccacttggt
    40261 ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat tgtcagccaa
    40321 tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac aaaataatcg
    40381 atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa atcgaaatgg
    40441 tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac ttgatgataa
    40501 aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg cgtccaagcg
    40561 atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc ttctttatag
    40621 tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg ctgatacttg
    40681 tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg gtaggcacta
    40741 atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa
    40801 atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc
    40861 actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat catcgtagcc
    40921 agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg acacctgatc
    40981 tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt cgatccccac
    41041 atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt tagattctaa
    41101 aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg gatgaatggc
    41161 tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag aaataggaca
    41221 cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg tttgctctga
    41281 ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg caacaaaggc
    41341 gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa taaaggctgg
    41401 taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca agccagcata
    41461 caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca gcttcaaggt
    41521 tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag tggacacagc
    41581 tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag cacctgattg
    41641 agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg tactgctcaa
    41701 ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca aaaatggctg
    41761 caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc attcagcccc
    41821 cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca acgctaaagg
    41881 ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg aaggcgaaat
    41941 tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa tcggtaaaac
    42001 tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg catcgccagt
    42061 agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat
    42121 tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca taaaataaaa
    42181 ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg atagtacacc
    42241 agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta tagctggaat
    42301 catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac tttcttgatt
    42361 gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt ccttgaaggc
    42421 tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca ccacactaat
    42481 tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt tgagttcaaa
    42541 acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc caacctgttg
    42601 caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa gcagtaaaaa
    42661 agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct
    42721 gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata gcacattatg
    42781 atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta tagggtaata
    42841 taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta aaataagtat
    42901 atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa attcccaaaa
    42961 tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt ctgctcttgg
    43021 gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa caatataggc
    43081 aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa tttcaatcat
    43141 tccgagatat aaaggtatcg atagaatgat ttgggctagt attcttaaaa attaactagc
    43201 aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat tccaccaatt
    43261 atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc ttttatattg
    43321 ttataacagt gaataacata aaaattcccg acaatcattg taatattttt ttgtcacctg
    43381 tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac acctttggga
    43441 gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa cacctcagca
    43501 tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt tatgttgata
    43561 gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt ttcatgagta
    43621 atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc
    43681 aatggttttg atgacttctc taatccagat gtacctgtca tttttgaaaa aataatacct
    43741 ataatagcca agacaacaat acctataatg acaaacttct tgttcatatt tactttattc
    43801 aactttttat ctcctatctc caaaataatt tgctcttatc cctcttatca gtacattgac
    43861 atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa aatcgtaccc
    43921 attttctcat ctttttctcg atataagtag cccataataa cagcaacagc accaaataca
    43981 acaggaataa atgctaacga tactaccata gaaatccaac caataatcaa atacagcttc
    44041 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
    44101 ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtagtgctcc acaaaactgg
    44161 caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat gtcttctcct
    44221 cctaaaatca ttcaattgct tattagttaa ctgtttactt tattaaatac atcagaaaac
    44281 agctcattca ttggtacact taatatctca gaaagcatct tcatttgata gggtttagga
    44341 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat tttttttgct
    44401 aactccgttt gagaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc
    44461 atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat
    44521 ataaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac tgagatgggg
    44581 gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga aaagctctcg
    44641 ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca gtagcaagat
    44701 gggaaaattc tgtaacagag cctaacctcg atagcttaac agctctttct agagccctag
    44761 gaacccctgt aagccatttt ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg
    44821 ctctctatcg ttctcttagc actgaagatg cagttcgaac aattgactat atgaagctat
    44881 taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata aataacccac
    44941 cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg ataacctgtc
    45001 tagtttaata caattgggtc ttttctcttc cttactctcc gtccatcatc tcttgttaca
    45061 cttgtaaatt gatacagagg aactatccca gctcctgata atatcaattc ttttattgtt
    45121 tcatctatcc ctgccccttt cgctaaatat attaaaaaac tactacttgc tttcacattg
    45181 tatagtttct taatatcctt agaagctcgt tcaagagtga atccttgttt aacaaagtag
    45241 cctctgtttt ttattaaagc atttttcacg ctgttatccc caacattacg taggcgttca
    45301 caaacagtgt taataagttc tttctctgtc attagatatg tcccttgcca cgataagccc
    45361 tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctgag ttctgatcgg
    45421 gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt aacccattca
    45481 taagaaaaat attgatatgt aaaatgaatt ttttccagaa gtggaaggat atcttcattc
    45541 aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt gcttctctta
    45601 tgattaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc gagcatcttc
    45661 aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat aagtgatagt
    45721 aacttattac atttatctat gtcataagtt ttaccatagt aagaactcat ttttttagct
    45781 aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa aaaattaaca
    45841 tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct tattgtcctg
    45901 aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc ctctttcaat
    45961 tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa tgtttctacc
    46021 cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact gatatcacaa
    46081 ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat actagcagag
    46141 ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag taatttattc
    46201 atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga ggaatcaaac
    46261 aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat caacttaaca
    46321 aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca atttttagaa
    46381 catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc ttcattaatt
    46441 attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc atttacattg
    46501 tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat tttaccatag
    46561 tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt ttcagagtac
    46621 cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga taaaaccttt
    46681 tctacgctta agtatctact atcatcttta ctcaaatcta ctaccttccc cttctcatct
    46741 ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact ctccttgtca
    46801 ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag tatctctaca
    46861 aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac atcagttttt
    46921 tctttttttg taactggctt ctctaataat ttttcttgat ctacataaat tggaatatta
    46981 atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata
    47041 cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt ttactcaacc
    47101 tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga ccgtatcaat
    47161 ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta ctgatacacc
    47221 tagatattca cttgcctgct gatagtccat atactctaga gtgactccag aaataatgtc
    47281 ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa cttgtataga
    47341 aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga ttgcttgaac
    47401 aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt tgttattaac
    47461 cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata cctcgataaa
    47521 aaaggggtgt caacagcttt gtaaaattta tttgcaacac cccttttttg aggtatacta
    47581 attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt agaacgaaaa
    47641 aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac acaaccagca
    47701 tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta cgaatgggag
    47761 aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt gggaaatatg
    47821 agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat tattctttat
    47881 agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt ccagcatctt
    47941 cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag atattccaaa
    48001 ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt taaaaatgaa
    48061 aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc tattgtagaa
    48121 gaatttgaca ataacattct tttcctcact agcagtatag aagaactact agaaagaatg
    48181 gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga agctgtgaga
    48241 gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg gaaaaaatac
    48301 actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct tgtaaactaa
    48361 gaggagatga tttaatggca aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt
    48421 ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata aatactacga
    48481 gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac agacatcaaa
    48541 tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg tataatgaat
    48601 ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca gtaagatacg
    48661 caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc tctgtcccat
    48721 attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat tatatgagaa
    48781 aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac aataatccta
    48841 tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa
    48901 aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg aaacaatacg
    48961 ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc gctttacagt
    49021 ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt gctgtagatg
    49081 aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga gttatctctt
    49141 tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa gaagagtatt
    49201 taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca gttaaaaaca
    49261 ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa aaatataact
    49321 tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta ttagaagcag
    49381 gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa gtgacattag
    49441 acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac tttgctgata
    49501 tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat acaggcaaaa
    49561 caaaaaagct agaaccctta tatatcaagg gttctagctc ctataacagg tttacccaac
    49621 actgccttcc atctcataac tgatcaatcg attgagttcg actgcatatt ccattggaag
    49681 ttctttggtg aatggttcaa caaagcccat gacgatcatt tcagttgctt cagattcggt
    49741 caggcctcgg ctcatcagat agtagagttg ttcttctgag atttttgaga ctttggcttc
    49801 gtgttctaaa gagacttggc tgttgtggat ctcattgaat gggatcgtat cggatttcga
    49861 ccattcgtcc atgatgatcg tgtcacattc gatatgagag atcgaacccg cactgttttt
    49921 ggcaaaggtt acttgtcctc gatagtttac ttctccgccg tcttttgaaa ttgatttaga
    49981 gacgatagag ctagatgtat ttggtgcatt gtggatcatt tttgcacccg tatcttggat
    50041 ttggtttgct cctgcaaagg cgatggagag catggtgcct cgtgcacctt ttccatctaa
    50101 gtaaacactt gggtatttca tcgttgtttt tgcacctagg tttccgtcga tccattcaac
    50161 ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag ttgtacacat tgtctgacca
    50221 gttttggatc gttgtgtaac ggcaatacgc atcttttctt gtaaagattt ctacgatggc
    50281 ggcatgcagg ctgttacttg aataagtagg tgccgtacaa ccttcgacat agtggatgct
    50341 tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat
    50401 ccggaagtac gtttgtaatg gaacatcgac tttgacgcct tttggcacgt aaataaaggt
    50461 cccacctgac catacggctg aattcagcgc tgctaatttg ttgtcggttg gcggtacaag
    50521 ttttgaaaag tactctttga ataattcagg gtattctttc aaagcagagt cagtgtcagt
    50581 aaagacgatt cctaactttt cgaattcatc tttcatatta tggtacacga cttctgattc
    50641 atattgtgcg gaagcaccgg ctagataagc tcgttcagct tctggtatac caatcttttc
    50701 aaatgtatct ttgatttttt ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg
    50761 tttttggtaa tatttgatgg catcgaaatc gatgtctgaa aggtcggggc cccatgtttg
    50821 cattggcatt ttgttaaatg tttctagaga tttcagacgg aattcaagca tccattctgg
    50881 ttcgcctttg attcttgaca tctcacggat cacttcttct gtcaagcctt ctcctgtact
    50941 gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc
    51001 catgttctca ctcctcttcc ttgtggtaat gatggaaagc tgcggtacca tcgttttcca
    51061 ttgcttttcc tagtgctttc caggcaagtg tcgcacattt gatccgagcg ggaaatttag
    51121 ctacaccacc aagcatagca gcatcgccta attggtcggg atctttgact tcttttcctt
    51181 ggacaagctg tagaaagtct acagttaaat gttctgcttc ttctattgat tttccgatga
    51241 cggcgtctgt catcatcgag gcacttgccg tactgatgga gcagccactg ccgtcaaagg
    51301 cgatattttt gatgattccg tcttttacat cgacttgcaa ttcgatcaca tctccacatg
    51361 tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat
    51421 gggaagaatg atccaagatc acttgacggt ataaattgtc taatttagat agtcccatgt
    51481 tggaaaaact cctttgttgc catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg
    51541 ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt caaggtactt caatagcggt
    51601 tgcgcacaat gatgacctgc tctgacggct accccttcca tgtctaaagc tgtcgcgaca
    51661 tcatggggat gtaacccatc caaattgaat gcaacgactc ctgtccgaaa attcgggtct
    51721 tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt ttggcaacac ataagcggct
    51781 agttcttctt catatcgatg gatattgtcc ataccgagtt tttgcaaata atcgattgca
    51841 tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt
    51901 tctttccatg tactgtcgta taattcgaca aaatcgatca tttcgccacc aaattcaaca
    51961 ggttccattt cttctaacca ttttcgttta ccatataaaa caccaatacc tgttggtccg
    52021 cacattttgt gtccgctgaa agcaaagaaa tcacagtcta gtgcttggac atcaatggcc
    52081 atgtgcgggg cagattgagc accatctact acgatgaccc cgccgttttc atggacaagt
    52141 tcagctaatt cttgaacagg attgaagaca cctaagacat tggatacatg agcaatggag
    52201 acgattttgg ttttgtccgt aatctttttc gctgcatcct ccatatccag gaagccttct
    52261 tctgttagtt cgatatattt caagacagca cctgttcgtt ttgccaactg ctgccacggg
    52321 ataacattag aatgatgttc catgtaagag agcactatct cgtctcctgc tttgatatag
    52381 cggtcgccga aggttctggc tacccaattc aagcttgtcg tcgtaccacg agtaaacagc
    52441 acttctgctg tttcttttgc atggatgaac gcccgtactt tttctcttgc ttgttcataa
    52501 tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat gaacattggc gttatcgtgt
    52561 tcgtaatagt acgctaatgc ctggagtact tgttttggct tttgggtcgt cgctgcatta
    52621 tccagataaa ctaaaggttc atcattgacg tcttgaaata agataggaaa atcattgcgt
    52681 agctttttag catcgatcat ccattcaact tcccttcgat tacttcgact aattctttcc
    52741 gaacttgttc tacagggatt gctgttaaga cagaaccgag gaatcctcga atcactaagc
    52801 gctctgcttc ttctttatgc aatcctctac tcatcagata gtacatttct tctggatcaa
    52861 ctcgtcctac actggcagca tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag
    52921 gattcgcatc accacgtgct ttatcagaaa gcatcaagac acgactttct tgttgggcat
    52981 ctgcaccttt tgcccctttc aagatgtggc cgataccatt aaaggtcaat gttccttttt
    53041 ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg aggggcttta ttggttactc
    53101 gtgtatcgat tccttggatt tgtctgcccg agctgattgc cacgattttg acttctgcgt
    53161 gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat cacatgaccg tcattcatga
    53221 caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg cccacggcga ttcatataag
    53281 aagtaatatt ttctcctaat tgatctacgg ctgaatattt gatttttgaa ccagctttag
    53341 cgatgacttc cacgatgata ttacctgatg attttgctac ttgttctttg gttgtctgga
    53401 aacgttccag ataagaaaat tcactatgtt catctgccac gatcaacaca tgcttgaaga
    53461 agtgctcatc ggatgcgcca tcttgaatga ataaggactc gagtggttca tcgatcacga
    53521 catttttagg cacatacagg aaaataccgc tgttcataaa agctacatgg agtgcagtca
    53581 attggtcttc gttcatgttt actgcttttt tcatataata ttcttggact agttctggat
    53641 attcgatcat tgcagtgaac aagtcggtaa aaatgactcc tttttctgct aattccacag
    53701 gcagttgttc aaaaatcgtc catgaacctt gttggacaag cacaggattg tctttcattg
    53761 catcaaatgc cggaatcgtg cctgtttcac tgatggtttg tgtttctttc acatcaaata
    53821 acgaccagcg atggaatttc acccgatcga tatgcggcaa tggcagttca tctgctttcg
    53881 ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc gcctttactt aaagaaaaat
    53941 catttaccgc gtcaagataa tcgagccagt ttttttcttt cattcttgct cccccttacg
    54001 cttcttcttc tttatagtcg attcctaatt ctttactgat accagcgtag ccttctgctt
    54061 ccagacgttt agctaggtca gcattccctg tcatgactac tcggccttcc atcatgatgt
    54121 gaacgacatc aggtgtgata tagttcaata atcgttgata gtgggtgatg atcaatgcac
    54181 cgaaattgtc tccgcgcatt tcatttactc ctttagcaac gactttcaaa gcatcgatat
    54241 ccagacctga atcgatttcg tctaaaattg caaatgtagg ttctaacatc aacaattgca
    54301 ggatctcatt ccgtttcttt tctccgccag aaaaaccttc gttcagataa cgttcagcca
    54361 tttcttctgg catatttagt aaagccattt tttcatctaa ctttttcaaa aatgccataa
    54421 cagaaatttt gtcgttttca tcacgtttcg cattgatcgc tgcacgcatg aattccgcat
    54481 tcgtgatacc tggtatctca cttggatatt gcatagctaa aaataagcca agtcgcgcac
    54541 gttcatctac ttccaagtct aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta
    54601 cttcatagtt tggattgccc atgatagcgg cagataaggt agattttcct gtgccgttag
    54661 gtcccatgat ggcatggatt tctccggttt tcattgttag gtttacgcct ttaaggattt
    54721 ctttttcctc gatcgacaca tgtagatttt ttatttctaa gacagacatg cattataccc
    54781 ccaaattctc tttcgttctc actcatttta gactaattga gaatgaagcg caatgacctt
    54841 ccttactaaa atcaaatatc ttttatttat tattaaacaa ttttgatttc taaattagaa
    54901 taggtataaa aaagatgaga aataagccat tacagactta tttctcatct tttttgttcc
    54961 ataccttatt ttgcatcagc ctttactggt ttaacagagc cgccccattt gtctgtgatc
    55021 cattcttgga cttctttgcg gtgaagcact tcaatcagtt ttttgatttt gtcactgtct
    55081 tcatcacctt tacgagtcgc aataatattc acgtaaggag agttgtcctt ttcaagcaat
    55141 acggcatctt ctaatggatt caaccctgcg ccgtaagcga agtttgcgtt gattgcaact
    55201 aggtcacctt catcattttg gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg
    55261 aacttcaagt tcttagggtt ttcttcaata tcatcgaatg ttgctgtttc cagatctact
    55321 ccatctttga ctttgactaa gccagcatct tgtagaattg tgatgatgcg tccccagtct
    55381 gattcagaat ttgaagtaat gattgttgca ccatctttta attcggaaac atctttgact
    55441 tttttagagt acaagcccat tggttcgatg tggacagcac cggcattgac aaagtcatat
    55501 cctttttctt tgatttcttt gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg
    55561 tcgccttctt ccaaagattg gttcggtaat acatagtcat cgaatttgac gatttcaaga
    55621 tcgattcctt catctttcaa taatggtttc acatgttcca aaatctctgc gtgcggtgtc
    55681 ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg
    55741 ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa
    55801 attttctttt tcatatccta catcccccta tatcctatta tcttttgtct gttttcttag
    55861 ttaagaaatc accgattcct tgaacgataa atacgattag taaaataatt aaagtcgcaa
    55921 cgaatgtcac tgttagatta ccacgctggt agccttcctg ccaagcaaga cctccaagtc
    55981 cacctgcgcc gatcgcgccg gccatcgcag taaatccgat catcgagatc gttgtaactg
    56041 ttaatccaga aatcaatgcg ggaatacttt caggtatcaa gaccttccag atgatttccc
    56101 agtaacttgc tcccattgca tctgctgctt ccagtacacc agggctgact tcacgaaaag
    56161 caatctctac tagacgggca taaaatggtg ctgcggataa aacaagagct ggaatggctg
    56221 cttgcgcacc tagcatagtg cctacgattg ctttagtaaa tggaatgatc aaaaccatca
    56281 ggatcataaa tggcgttgac cggaaaatat tactgatgat cgatacgatt ccataaatga
    56341 ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc
    56401 cgatgatcgt cactaagacc attgagatta atgtcatata tagtgtatca aggatagcct
    56461 gattcatcgt ttccgggttg atttgtttaa aatctagata tgtttctgaa aagcttttat
    56521 ccacgatgga tcacctcaat tcctactcct tgttctttga tgaacacttc tgcttgatct
    56581 aattctttgg tttcaccagt catcaagaca gtcaatgaac caaaggaacc ttctttcact
    56641 tgtttgatct ttccatacac aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa
    56701 atgactggct cgttggcatt tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga
    56761 ttttctttga tgagttcagc taatagttct gtcgaatcag cttctggttc gatgtcttgt
    56821 tgtacaaagc gtttggttac ttcttttttc ggttgtctga agacagtcaa tacgtctcct
    56881 tcttctacga ctttccccaa ctccatcaca gcgactttgt tgcagatttt gcggatcaca
    56941 ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt tcttattgat cgctaacaat
    57001 aagtctaata cttcttctgt tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc
    57061 aggatctcag gatcatttgc caatgctcgt gcgataccga cgcgttgttt ctgtccgccg
    57121 gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc cgactaaacg caacagttct
    57181 tctgcccgtt ctttacgttt gctctttggt acgccagcta attccagcgg cagctggata
    57241 ttttcaagta cagttcttga ccataacagg ttgaaatgct ggaagatcat accgattttc
    57301 tttcggaatg tacgtaattc tttgtttttt aattttgtaa tatcttgatc gtttacgatc
    57361 acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac ggactaatgt actttttcct
    57421 gcgccagaat agccgacgat tccataaata tccccttttt caacgtttag tgaaacgtca
    57481 ttgacggctt ggatgtttcc gtttttccca ttgaatgtct ttttgacatt cgttagttgg
    57541 attaatgaca tgccattcac atctttctct tatgattttt caaacaaaaa agccttcgcc
    57601 ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt gttaccacct tgatttaccg
    57661 ttacttcgca ataacagcct cttccagtac ttggacttag cttctttccg tatactgtcg
    57721 cgctgtaacg ggcactcccg tagtaagttt gcttacgctc gcttactctg ctctaagacc
    57781 atcttccatc tcattttcgt tacccatttt cagcaaacag ggctctcttc aaacgtccgt
    57841 gacatgtact cttcttttca aagctttctc gtttcatata ggattgctta tgattatagc
    57901 aactggaaga atcagtgtca acttttttca agtgattttc tgattttata attcgtcaaa
    57961 ttcttttgga tcaacatctt tgaaacttaa gatccacgca ttcatttcat ctgaatcatt
    58021 caagatattg atatcttcac cgattttatc attaacagaa gaaactgtac ccgttagcgg
    58081 gctggcaaat tcattgaccg ctttttccgc ttcaacttcg atcagaggtt catctttttt
    58141 tagcgattgt cctgcttttg gcagattggc aaacgtgatg tctccaagtt cttcttgcgc
    58201 ttcatttgtc aaaccaacac agtattcttt tccattaaat aagatccaaa ggttgtcctt
    58261 tttcttcaaa tgcttgcctg ccattttata tcagttctcc ttcataaatt ttttcattga
    58321 atccaattgc aacaagctgg tcctctcgta agatcagtgg tcgcttgatc agcataccat
    58381 ctgtcgataa gacttggctc gcttttgaag ccgtaaactc gcctacttga tctttcagtc
    58441 caagttcccg atatttcatc ccgcttgtgt tgaacaggcg acggattggt acttgactcg
    58501 tttctagcca agtctcaatc gttgaggcct tcggtacttc tttgatcata tcaacggttt
    58561 gataactaac gccatgctgg tctaaccacg ctttggcttt tttgcatgtt gaacatttcg
    58621 ggtaccagta aaaggtgtac aattttctta cctccttgaa gatcttgcag gtgcttcatt
    58681 ttggtatctc atcgataata tcaacccgat accgatcatg ttccctaaaa tcgaggatcc
    58741 tccctgacta atgaaaggca atgggatccc tgttaaagga agtaatccga tatttgctcc
    58801 aatattctca aagacatgga acagcagcat catgatcaaa ccgcttgcga tataggcgta
    58861 aaattcatta ttcgtatcga aacacacccg aatcatacga tagattaaaa taaaatacaa
    58921 gaaaatgacg aatgcaccac cgataaagcc gaagttttca ccgataacgg taaaaatcat
    58981 atcggactca cgaacaggca catagatatt gcttacgttg aatcctttac caaacaagcc
    59041 accggtacca attgccaaaa gtccttgggc cggctgataa ctcattcctg acgtatcatg
    59101 gaaagggtcc agccatgaat caatacgtgc aaactgataa gatttgaatc caactttata
    59161 aagcaagtct cttccgcctt cagtcgtaac caagaagatc gttcctgctc caacgactac
    59221 tgccaagatt actacaggtg cgatgatgcg ccaagagatt ccagacatca ggaaaatccc
    59281 gccaaagatc gcaagaaaga caagcatcgt tccaaagtca tcttgggcca tgatcaatgc
    59341 taaaaccggt attgtcacga tcaagagctt cccaatcagc caaaagtccg atttcatcgt
    59401 ccgttctttt acttgcgtat tatgtttagt tacgatcagt gccatcatca agatgtacgc
    59461 aatcttcatc aactctgctg gctggaaagt aaacgcaccg aatcgaaacc agtttttaga
    59521 ccctgttgaa gcagctaaat tctgatcata gaatttgagc aagagtccca ttaccactaa
    59581 ccccaaacca taaaggtaag gagtcagttt ccagatccat ttggaattca aatgcatgat
    59641 gacaacgatt gctgccgctc caactaaata ccagacaact tgcatcccta cgcctttcaa
    59701 gactgctgga ttgttcggat catggctaag tgctacatat aatgagagca gaccaatcaa
    59761 acataacaga aagactggta aaatcacgcc ataatcaata cgattatcgt tcgaagcttt
    59821 taattttttt tccatgtttc atcccttttc ttaaaaacca tactgctgat tattatagcg
    59881 gtccagttag ataatggaaa gtctttccat agacatttgg gaaaaattaa ctttccgttt
    59941 gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc ttatctttag tgtatcatag
    60001 aacttttttt gatacatgca attatctacc agctgagatt ctcctcgctg tgagaagttt
    60061 ctgtgctttc attgtatcga aggatctctt cggcaatctc tagcagctga tcagaaacca
    60121 gattggttcg ttggatgaaa taaaaatatc gatggaaatt caacggagtc attggcacat
    60181 tttctggaac agagcgctta gacaaaatcg agcgaccgat tccttccttc agcaacgcaa
    60241 caattacatc gttacttttc acgatcatct tcttatcttg gatattcatc tcttcaaaat
    60301 aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct tacaagccac aatttgcttt
    60361 ttgggtcccc agcgatgact agctggtctt ccatcaaaga gtatctttgt acgccggaag
    60421 tctctaatgg cttttcgata aaaccaaaat cggattcatg tttcgctact tgttctaatg
    60481 attcatgaga attcgtgatt tcgatctcaa aatgcacttt aggaaacctt ttgatcagat
    60541 gtttcatcaa ctgaggcaaa agatagacag caaaggtgtg ggaagcaacg atccgacata
    60601 cttcttcttc attttgttca ttgagcaatt tttgctgagt ttcttcccat tcttctaaca
    60661 aaaccagtgt ccgttcatat aataagtcgg cttgcttagt tggaagcatc tcttttcttc
    60721 cgttacgttg aaaaagatcg atattcaatt cccgctccaa ctgtttgatt tgattcgaga
    60781 ctgctggttg agataaaaac agtacttctg ctgctctgga aaaatttctt gtttcatata
    60841 ctgctcgaaa agtcgttaat aacttaaaca tggcaccact tccataactt tttatgataa
    60901 ttactataaa aactatttga tttaaatata caaaaaacca tctataatag aaatgtacaa
    60961 caaaacagtt gctgatccaa ctagtaactt agatgaagac aatcagctgt ctgcttgctc
    61021 gtaatcatac attcctattc attttacaat gtattcgttc atcttggtac taaaatctat
    61081 taaataaaaa ggagaatatt atatatgaag aactttttga aaattattcc gatcccgatc
    61141 tgcgggttga tccttgggtt agcttccctt ggaaatctgt ttaaagacta tcaccatgtt
    61201 gctttaggca atctaactgg cggtatctct atgattctta tgatattcat ttttgttaaa
    61261 ttgtttatct tattcgaaca cacgaaacaa acgttgcatg atccaatcat cgcctccgtt
    61321 tcaccaactt ttacgatgtc actcatggtt atttgtactt attttgtttc atatgaaagt
    61381 attgcaccaa ttgtcaaatt catttggctg tgcgctgtta tcttccaagt gatccttgtc
    61441 ttttatttca attatcacca tgttatcaaa gctgatcttt caattgaagc aatttatcca
    61501 agctggttta tcatttacgt tggatttggg gtcatcactg taactgccgg gaacttcttc
    61561 ccacttattg gaaaaatttt cttttggatt tcattgatct gctatgcgtt cttattgccg
    61621 atcatcatca aacgtgtctt tttcgtaaga aatatggctc atccgacacg tccgctgatt
    61681 acgatcgttg cagcacctgg ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa
    61741 cctaattttt atcttgtcgt agcattgttc gctttatctc aagtattgta tttcgttgtg
    61801 ctgtctatgc ttccacgttt gttcaagctt cgtttttatc caagctatgc agcctttaca
    61861 ttcccgcttg ttatctctgc aacagctata tttactactg ttcattactt caacagttta
    61921 ggtatctata gtagcgtatt ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta
    61981 gttgtcttct acgttcttgc tcattacgta cgtttcttaa ttaaagaaca tagcaaacat
    62041 ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc aataggttgt gaaaaagctg
    62101 ttctgcatca agtaataaga acggccaaac aaaaacagct gttcatgttt ttgtactggt
    62161 tgggcttatt accgcagatg caagcttttg aacaccgttt accaagaggt cgtaa
//
LOCUS       DACECS010000008        60193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.008, whole genome shotgun sequence.
ACCESSION   DACECS010000008 DACECS010000000
VERSION     DACECS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.008"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWO31_000525"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWO31_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0300968.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWO31_000526"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWO31_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0300969.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWO31_000527"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWO31_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0300970.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWO31_000528"
     CDS             1642..2274
                     /locus_tag="IWO31_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0300971.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWO31_000529"
     CDS             complement(2276..3181)
                     /locus_tag="IWO31_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0300972.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWO31_000530"
     CDS             complement(3266..3919)
                     /locus_tag="IWO31_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0300973.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWO31_000531"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWO31_000531"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0300974.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWO31_000532"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWO31_000532"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0300975.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWO31_000533"
     CDS             6168..7253
                     /locus_tag="IWO31_000533"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0300976.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWO31_000534"
     CDS             7250..8314
                     /locus_tag="IWO31_000534"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0300977.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWO31_000535"
     CDS             complement(8563..9366)
                     /locus_tag="IWO31_000535"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0300978.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWO31_000536"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWO31_000536"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0300979.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWO31_000537"
     CDS             complement(10244..11251)
                     /locus_tag="IWO31_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0300980.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWO31_000538"
     CDS             complement(11258..11902)
                     /locus_tag="IWO31_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0300981.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWO31_000539"
     CDS             complement(12002..12760)
                     /locus_tag="IWO31_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0300982.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWO31_000540"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWO31_000540"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0300983.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWO31_000541"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWO31_000541"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0300984.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWO31_000542"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWO31_000542"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0300985.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWO31_000543"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWO31_000543"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0300986.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWO31_000544"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWO31_000544"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0300987.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWO31_000545"
     CDS             18158..19027
                     /locus_tag="IWO31_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0300988.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWO31_000546"
     CDS             complement(19153..20052)
                     /locus_tag="IWO31_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0300989.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWO31_000547"
     CDS             complement(20217..21041)
                     /locus_tag="IWO31_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0300990.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWO31_000548"
     CDS             complement(21052..22347)
                     /locus_tag="IWO31_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0300991.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWO31_000549"
     CDS             complement(22363..22842)
                     /locus_tag="IWO31_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0300992.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWO31_000550"
     CDS             complement(22848..23876)
                     /locus_tag="IWO31_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0300993.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWO31_000551"
     CDS             complement(23893..24999)
                     /locus_tag="IWO31_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0300994.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWO31_000552"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWO31_000552"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0300995.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWO31_000553"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWO31_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0300996.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWO31_000554"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWO31_000554"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0300997.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IWO31_000555"
     CDS             29970..30668
                     /locus_tag="IWO31_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0300998.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IWO31_000556"
     CDS             complement(30836..33475)
                     /locus_tag="IWO31_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0300999.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWO31_000557"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWO31_000557"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0301000.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="IWO31_000558"
     CDS             35318..36493
                     /locus_tag="IWO31_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0301001.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="IWO31_000559"
     CDS             36497..37138
                     /locus_tag="IWO31_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301002.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="IWO31_000560"
     CDS             37135..38052
                     /locus_tag="IWO31_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0301003.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="IWO31_000561"
     CDS             38049..38720
                     /locus_tag="IWO31_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301004.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="IWO31_000562"
     CDS             complement(39261..39749)
                     /locus_tag="IWO31_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0301005.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="IWO31_000563"
     CDS             complement(39950..40216)
                     /locus_tag="IWO31_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0301006.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="IWO31_000564"
     CDS             complement(40216..40479)
                     /locus_tag="IWO31_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0301007.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="IWO31_000565"
     CDS             complement(40472..40744)
                     /locus_tag="IWO31_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301008.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="IWO31_000566"
     CDS             40955..41140
                     /locus_tag="IWO31_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0301009.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="IWO31_000567"
     CDS             complement(41282..43105)
                     /locus_tag="IWO31_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0301010.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="IWO31_000568"
     CDS             complement(43703..46408)
                     /locus_tag="IWO31_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0301011.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="IWO31_000569"
     CDS             complement(46714..47661)
                     /locus_tag="IWO31_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0301012.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="IWO31_000570"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="IWO31_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0301013.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="IWO31_000571"
     CDS             complement(48296..48658)
                     /locus_tag="IWO31_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0301014.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="IWO31_000572"
     CDS             complement(48669..49790)
                     /locus_tag="IWO31_000572"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0301015.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="IWO31_000573"
     CDS             complement(49806..50156)
                     /locus_tag="IWO31_000573"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0301016.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="IWO31_000574"
     CDS             complement(50286..51797)
                     /locus_tag="IWO31_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0301017.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="IWO31_000575"
     CDS             complement(52119..53471)
                     /locus_tag="IWO31_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0301018.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="IWO31_000576"
     CDS             complement(53624..54700)
                     /locus_tag="IWO31_000576"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0301019.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="IWO31_000577"
     CDS             54896..56641
                     /locus_tag="IWO31_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0301020.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="IWO31_000578"
     CDS             complement(56921..57358)
                     /locus_tag="IWO31_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0301021.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="IWO31_000579"
     CDS             complement(57342..58715)
                     /locus_tag="IWO31_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301022.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="IWO31_000580"
     CDS             complement(59037..59525)
                     /locus_tag="IWO31_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301023.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="IWO31_000581"
     CDS             complement(59547..60149)
                     /locus_tag="IWO31_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0301024.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10988 g        19085 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tttatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ctt
//
LOCUS       DACECS010000009        58822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.009, whole genome shotgun sequence.
ACCESSION   DACECS010000009 DACECS010000000
VERSION     DACECS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.009"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IWO31_000582"
     CDS             83..1081
                     /locus_tag="IWO31_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0301025.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IWO31_000583"
     CDS             1105..1389
                     /locus_tag="IWO31_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301026.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IWO31_000584"
     CDS             1470..1868
                     /locus_tag="IWO31_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0301027.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IWO31_000585"
     CDS             1878..4421
                     /locus_tag="IWO31_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAQ0301028.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IWO31_000586"
     CDS             4443..6785
                     /locus_tag="IWO31_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301029.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IWO31_000587"
     CDS             6801..8975
                     /locus_tag="IWO31_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAQ0301030.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IWO31_000588"
     CDS             8965..9387
                     /locus_tag="IWO31_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0301031.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10268..11179
                     /locus_tag="IWO31_000589"
     CDS             10268..11179
                     /locus_tag="IWO31_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301032.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11666..11932
                     /locus_tag="IWO31_000590"
     CDS             11666..11932
                     /locus_tag="IWO31_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301033.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12004..12345
                     /locus_tag="IWO31_000591"
     CDS             12004..12345
                     /locus_tag="IWO31_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301034.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12400..12819
                     /gene="ssb"
                     /locus_tag="IWO31_000592"
     CDS             12400..12819
                     /gene="ssb"
                     /locus_tag="IWO31_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0301035.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12896..13192
                     /locus_tag="IWO31_000593"
     CDS             12896..13192
                     /locus_tag="IWO31_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301036.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13207..13638
                     /locus_tag="IWO31_000594"
     CDS             13207..13638
                     /locus_tag="IWO31_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301037.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13820..14125
                     /locus_tag="IWO31_000595"
     CDS             13820..14125
                     /locus_tag="IWO31_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301038.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14529..15017
                     /locus_tag="IWO31_000596"
     CDS             14529..15017
                     /locus_tag="IWO31_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0301039.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            16351..16653
                     /locus_tag="IWO31_000597"
     CDS             16351..16653
                     /locus_tag="IWO31_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301040.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            16646..17674
                     /locus_tag="IWO31_000598"
     CDS             16646..17674
                     /locus_tag="IWO31_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0301041.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            17730..17948
                     /locus_tag="IWO31_000599"
     CDS             17730..17948
                     /locus_tag="IWO31_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301042.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            18019..18834
                     /locus_tag="IWO31_000600"
     CDS             18019..18834
                     /locus_tag="IWO31_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301043.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            18898..19233
                     /locus_tag="IWO31_000601"
     CDS             18898..19233
                     /locus_tag="IWO31_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0301044.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            19657..19920
                     /locus_tag="IWO31_000602"
     CDS             19657..19920
                     /locus_tag="IWO31_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301045.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            20236..20666
                     /locus_tag="IWO31_000603"
                     /pseudo
     CDS             20236..20666
                     /locus_tag="IWO31_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            20805..21227
                     /locus_tag="IWO31_000604"
     CDS             20805..21227
                     /locus_tag="IWO31_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301046.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            21372..21617
                     /locus_tag="IWO31_000605"
                     /pseudo
     CDS             21372..21617
                     /locus_tag="IWO31_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21726..22682
                     /locus_tag="IWO31_000606"
     CDS             21726..22682
                     /locus_tag="IWO31_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301047.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(23518..24189)
                     /locus_tag="IWO31_000607"
     CDS             complement(23518..24189)
                     /locus_tag="IWO31_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0301048.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            24506..26137
                     /locus_tag="IWO31_000608"
     CDS             24506..26137
                     /locus_tag="IWO31_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0301049.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            26140..27219
                     /gene="uxuA"
                     /locus_tag="IWO31_000609"
     CDS             26140..27219
                     /gene="uxuA"
                     /locus_tag="IWO31_000609"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0301050.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            27293..28720
                     /gene="uxaC"
                     /locus_tag="IWO31_000610"
     CDS             27293..28720
                     /gene="uxaC"
                     /locus_tag="IWO31_000610"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0301051.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            28804..29451
                     /locus_tag="IWO31_000611"
     CDS             28804..29451
                     /locus_tag="IWO31_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0301052.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            29580..30956
                     /locus_tag="IWO31_000612"
     CDS             29580..30956
                     /locus_tag="IWO31_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301053.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            30967..31761
                     /locus_tag="IWO31_000613"
     CDS             30967..31761
                     /locus_tag="IWO31_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0301054.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            31774..33540
                     /locus_tag="IWO31_000614"
     CDS             31774..33540
                     /locus_tag="IWO31_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0301055.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            33568..35376
                     /gene="uidA"
                     /locus_tag="IWO31_000615"
     CDS             33568..35376
                     /gene="uidA"
                     /locus_tag="IWO31_000615"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0301056.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            35548..35949
                     /locus_tag="IWO31_000616"
     CDS             35548..35949
                     /locus_tag="IWO31_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0301057.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            35943..36188
                     /locus_tag="IWO31_000617"
     CDS             35943..36188
                     /locus_tag="IWO31_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301058.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(36512..36733)
                     /locus_tag="IWO31_000618"
     CDS             complement(36512..36733)
                     /locus_tag="IWO31_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301059.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(36895..36967)
                     /locus_tag="IWO31_000619"
     tRNA            complement(36895..36967)
                     /locus_tag="IWO31_000619"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36932..36934),aa:Lys,seq:ctt)
     gene            37043..37273
                     /locus_tag="IWO31_000620"
     CDS             37043..37273
                     /locus_tag="IWO31_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301060.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            37317..38486
                     /locus_tag="IWO31_000621"
     CDS             37317..38486
                     /locus_tag="IWO31_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0301061.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            39060..39629
                     /locus_tag="IWO31_000622"
     CDS             39060..39629
                     /locus_tag="IWO31_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0301062.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            39616..40476
                     /locus_tag="IWO31_000623"
     CDS             39616..40476
                     /locus_tag="IWO31_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0301063.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            40666..41370
                     /locus_tag="IWO31_000624"
     CDS             40666..41370
                     /locus_tag="IWO31_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301064.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            41375..43210
                     /gene="walK"
                     /locus_tag="IWO31_000625"
     CDS             41375..43210
                     /gene="walK"
                     /locus_tag="IWO31_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0301065.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            43207..44523
                     /locus_tag="IWO31_000626"
     CDS             43207..44523
                     /locus_tag="IWO31_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301066.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            44524..45393
                     /locus_tag="IWO31_000627"
     CDS             44524..45393
                     /locus_tag="IWO31_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301067.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            45448..46257
                     /locus_tag="IWO31_000628"
     CDS             45448..46257
                     /locus_tag="IWO31_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0301068.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(46360..46989)
                     /locus_tag="IWO31_000629"
     CDS             complement(46360..46989)
                     /locus_tag="IWO31_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0301069.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            47159..48451
                     /locus_tag="IWO31_000630"
     CDS             47159..48451
                     /locus_tag="IWO31_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0301070.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(48527..49189)
                     /locus_tag="IWO31_000631"
     CDS             complement(48527..49189)
                     /locus_tag="IWO31_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0301071.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            49414..49698
                     /locus_tag="IWO31_000632"
     CDS             49414..49698
                     /locus_tag="IWO31_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0301072.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            49749..51374
                     /gene="groL"
                     /locus_tag="IWO31_000633"
     CDS             49749..51374
                     /gene="groL"
                     /locus_tag="IWO31_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0301073.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(51463..52611)
                     /locus_tag="IWO31_000634"
     CDS             complement(51463..52611)
                     /locus_tag="IWO31_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0301074.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(52713..53297)
                     /locus_tag="IWO31_000635"
     CDS             complement(52713..53297)
                     /locus_tag="IWO31_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0301075.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(53424..53855)
                     /locus_tag="IWO31_000636"
     CDS             complement(53424..53855)
                     /locus_tag="IWO31_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0301076.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53873..54193)
                     /locus_tag="IWO31_000637"
     CDS             complement(53873..54193)
                     /locus_tag="IWO31_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0301077.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54931..55188
                     /locus_tag="IWO31_000638"
     CDS             54931..55188
                     /locus_tag="IWO31_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0301078.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(55159..55467)
                     /locus_tag="IWO31_000639"
     CDS             complement(55159..55467)
                     /locus_tag="IWO31_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301079.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            55545..56291
                     /locus_tag="IWO31_000640"
     CDS             55545..56291
                     /locus_tag="IWO31_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0301080.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            56306..56509
                     /locus_tag="IWO31_000641"
     CDS             56306..56509
                     /locus_tag="IWO31_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301081.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56614..56913
                     /locus_tag="IWO31_000642"
     CDS             56614..56913
                     /locus_tag="IWO31_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301082.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            56913..57095
                     /locus_tag="IWO31_000643"
     CDS             56913..57095
                     /locus_tag="IWO31_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301083.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(57101..57577)
                     /locus_tag="IWO31_000644"
     CDS             complement(57101..57577)
                     /locus_tag="IWO31_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301084.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            57630..58355
                     /locus_tag="IWO31_000645"
     CDS             57630..58355
                     /locus_tag="IWO31_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0301085.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            58529..>58822
                     /locus_tag="IWO31_000646"
     CDS             58529..>58822
                     /locus_tag="IWO31_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301086.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT        20589 a         8797 c        11712 g        17724 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagtt ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacacaaact tacaagagag gggagtaaag cagttgcaga
    10201 ggaatcagga agatttgttc gaacagaaac ttgcaagaaa gcattattaa attgaaggtg
    10261 atgaaatatg aagtatttat tgcatggcta ttttattgat tattctactc gcaatagtag
    10321 tactttagcg attctatgtt tgcttcatga attgagagtg gttactagaa aacagctata
    10381 caaactattg aattttgaca gatcaattag taaacaagga gtaacgaatg ctttagtgag
    10441 attatatgaa aaacagttaa tcgatcgaaa aaaatatgga acagaaatgt attattattt
    10501 gacgaaagag ggacatcaaa gcataggtgg gtattatacg ttgccgaaag ttcctgaata
    10561 taatttgaat caccatttac aaattaacaa ttatttgatt aaaggaatgg cattggcaaa
    10621 agaccaccca catttaaaac ggattatttc agaaagacgt accgtctatg aaacgaagga
    10681 tttaaatcct aagacaaaag gaagaaaatt ttttgtgcca gattttattt ttcgttttcg
    10741 agacaaagaa aatcatgaga tcaattggtc ctttgaaatt gagctaacgt tgaaaacacg
    10801 tcgtcgctac cgtgaagcga tttttccgaa atatattcgt gagttgcgaa cacgtcctga
    10861 atcacattta atttatgtga cacctagtgg cgcaattcaa gaagaacttc atgcgtttaa
    10921 gcgtattttt ttcaagcgcg aaactgaaaa agatgcgagt caacaagaat tgtttgagcg
    10981 gttgcatatt ttttcagctt tagagtttga agagggatta aaagcattat tagagaatga
    11041 tccagcagtc aattggcgga cagtagaacg agaaaaaatt tatccgaatg gcgctgttca
    11101 aagtcgtttg aatttatttg aagaatcaac tgaaaatgag gaaaaaccaa aagtgaagtt
    11161 tacgcctgaa gtattgtaaa agagggtgtt cgcttcgctc tttttttgtg ccagtttgct
    11221 ggcaccgttc agacgcaagc gtctgaaccg gctcgttttt aggaaggact aacttgtttg
    11281 atttatccgt tgtcaaagaa gtggacgttc gagagtccct aatcgtatag cagtcattcg
    11341 gcgagtataa cctaaaacat aacctgccaa ttgccaaggg taaaatgcac gctgcgcgcc
    11401 cttggcaatc gtcagaacat gttttcgagc cggttatgcc gaacgaccgc tatacgaaaa
    11461 gggcctctaa acagaagtcc actttgatcc ttgacaactg aaacccaaaa tcaaacaagc
    11521 aggccttcgt ctaaaaacga gccgaatgaa gaccttgggg gttgcacccc caaacccccg
    11581 tgcccccgac acactaccga aaaggtgggg gtgggtggcg aaaaaatcaa ataaaacatt
    11641 ctattcaaga aaggaagaaa caaccatgac aactaaaaac gagcaactga aagaacaggc
    11701 aagaaaagag ttgcaagaaa gaggtttgat tattgagggg agctttgaag gagattttga
    11761 aacctatatt ggttgttatg caagaccgat aaacaaacct acagccttag acccaatgaa
    11821 cgaacaagaa gtaaaagaac aagaaaaaca tgcagtgaat ggttttccgc aagatttcac
    11881 tgaatggtat gagtgggaaa ttaacaacgg gaagcttgaa aacttcttat aaaagtcaga
    11941 tgacgtgcaa agagaatgat tctctttgta cggtaaaaat ataaagaaag aaggaaaaag
    12001 aaaatggcaa aaaacacacc ttatcaagta ggagacattt tttatagtag ttggggctac
    12061 gaacaaacga acgtgacttt ttggcaaatt gtgaagttaa cagaaaaaac agcatggttt
    12121 cagcctattc aaaagaaaac ggtcgagcat tattcgtcca tgagtggaaa agttgttcca
    12181 atgaaagaca agtttataga gtatccttta gcaaaaacta aagacagtat tgtaaaaaaa
    12241 cgaattgatc cagaaaccta tcctgatatg ttatgtggtt atcgtagttg ggacacgttt
    12301 catagatata aaaatcaagc tgtttatgaa agtagttatt attaagggga tagtttcccc
    12361 tttcaaaaat aataaataga agaatggaga gaaaaaatca tgatgaattc agtgaattta
    12421 gtaggacggt taacaaaaga agtggattta aaatacacac caaaaggcaa tgcgacaggc
    12481 acatttattt tagctgttaa tcgaaattat acgaatgcaa acggcgagcg tgaagcagat
    12541 tatatccgct gtgtcatttg gcggaaagct gcggaaactt tagcaaaata tactggaaaa
    12601 ggaagtttga ttggcattaa tggacgtctt caaacgagga gctatcagaa tcagcaagga
    12661 cagaccatgt atgttacgga agttctagtg acagatttct atttgttaga gagtaaagaa
    12721 atcaatgagc aacggtcaaa atcaatggcg aataatcaaa ataattcttc aattccagga
    12781 aatgagattc aaatttcaga tagttatttg cccttttaag tgaaaaataa ttttgttcta
    12841 tacctgattt cttcaggtat agaacaaaaa ataaatagag atgaggcgat tttttatgaa
    12901 aatcattgac agttacgatc aacgaaccat gcaatatttg ggctcgtttg attatacaga
    12961 tgaaaatatt attaattatg ttgcaagttt acctccttat caaagcgttc gcttggtaga
    13021 ttttgcaagt gatgaattgg tattaacaac gatgggcaac tttttagatt atgtcggaga
    13081 caaaaaatgg ttggaaaaaa ttttgtctaa attgattgcg aaacaaacag gagaagaacc
    13141 cataaatgcg attcaattgt tcaatcgata tgaacaagaa aacaatgatt aaagaggaga
    13201 gaaaaaatga aaagattaaa acgaagtatt tttattgtgc tagcattgat tgattttgtt
    13261 gggacaggtt gtgaagcaat tgacgttcaa aaagctccct tatcggaaat aaaacaagca
    13321 ataacaaaac aagatgaaga acaaccgcta aatacacaag tagaaatagt gaatcaaaat
    13381 aaaccgagtt tttctgaaga agatttttcg ttagaaaatg gaaccaggca atttttttca
    13441 gatttggatc gtttaaatcg ggtaggtgta gcaagtgcac ttttgcataa aagcaggatg
    13501 ccaacagaag aacgagaccg attatacatt aagccaactg gctggaaaca aaagaaaatg
    13561 aagaatggcg attatcttta taatcgttgt catttgattg gctatcaacg tgcgacaagt
    13621 ttccccataa gtatgtaata cgaaacggga ggattaatct taagattaat cacaactaac
    13681 ctatttgaca gttggaatga aagctatcat gtctgctgaa tcaactggtc tgatactcct
    13741 acatgcaata aagtctacta ggaagttatt gtatgaagct aggtaaaggc ggcagaacaa
    13801 tttctaagtg tggaatcata tgaagttgga tatgccggga cgctataaat tactcagggt
    13861 gagactgttt ttaattaaac tgacgaactt gcgaatgtac gggtctataa ccgacttata
    13921 ttcgaaagat ataagattgc gaatgcaaag aacattatcg aaaagtaaga atgttggtca
    13981 tgaaattcga gatggaagac tagcggtgta tctgtcaaga gttggtaaaa catgtttgat
    14041 tgattcagat gagagtggga atgcgacaaa acgttttaca gaagaaatag agcaactagc
    14101 agaactgaat atacgatatt tttaaatact ttagcggaga ataaaaaaat cctctacaga
    14161 aaactacgga gggcaacatg tgctattgtt tcaacaatac atttttaatt tttaccataa
    14221 caaaaaagta cttgaaaaaa aacagaaata tgttaaatta aaaatgaaaa aatggaagcg
    14281 gttacttatt cttttgaatc taagataaaa attcaaaaga ataacataac ccaaatatga
    14341 ttatacttga gattctaaat atttatcgaa aaggaatttg ttttaaggaa aaatgattct
    14401 atggacggtt attgatttat atttaccaaa tggctaataa aatagtattt cttatattgt
    14461 atagtttttg tttttatgtg aaaacatgag caatagaatt gtactagtag gataaggatg
    14521 gatggaaaat gaaaaaaact atttttgttt tagcgatttt tatatgtttt gggctacttt
    14581 tggtaggcta cttgttgttt aattctaatg aaatgagaac tatttctacg aatgacttgt
    14641 taaaaaaaag tgaattaaag gataaaaaaa aagaatctct tgcctttata atgtttactc
    14701 aagaagagtg tgagtattgt atagcaataa atccaatcat agatagtgta aataaggaaa
    14761 ctttgaataa gataaagaaa tatgatatat ctgtagattt tcaatctaaa caaaagtgta
    14821 aaattaagtt tgttccgact attgttgcat atagtaagaa taaagaagtg gggagaattg
    14881 acaatgaatt agataatttg tatcgaaaaa agcaagatgg ttcaattgta atagacaaga
    14941 aaaaaacaaa gacattgctt atagattgga tgaaaaaaat ggataaacaa ggagaatatt
    15001 tgtatggaga agattaaaca gacatttccg ttaattaata aatattggaa taagaaaaaa
    15061 atttttatat tttttattac gtttctgctg tcatctttat tttctctaat tttcccgtat
    15121 ctatttagta atgttattga tttatattta gagaatgagt tatcacagaa tattgtgcga
    15181 atagtaacga ttcttatatt tcagttattt ttagtcttaa ttaaagaagt gtatggatat
    15241 ataaaaacta tttatgcatt tgaagttgat ttatcaatga aaattgattt tcttaaggtt
    15301 ttacaaaaaa aaaatctttt tttttcgcaa acgaagcatt caggagaaat tcaatttagg
    15361 atgttttcgg atatttctac tatcgtaaat actatcttta ttttttctgt tgaattacct
    15421 gtgataggcg ttttagttct tgtagcggta tttattataa gtaaagtgaa tcttattttt
    15481 ctgatttttt cactaataat gatgggagct atggtgttac agtataattt atttcaatta
    15541 ccgcttaaga aaacaatcga gaatacaaaa agtgttaatc aaagtattac aggagaattg
    15601 aatgcacatt ttgatcaatt agaaattata agcgcatata attttgagaa tatctctatt
    15661 ttaaaattcg attctagatt taaagaatta tttaatgttt tcaaaaaaca aaataaatta
    15721 ctaacactgg gaagctgttt ggcacaggtt attttccaat tatggattgg aggaatattt
    15781 cttattgatg ttattttatt agctaaagga acaataagta ttactaattt tgtaatagta
    15841 tttatgtacg ctaacatggt ccctacttta tggactactt taattagtat tatttggaaa
    15901 tggcaagact attcagtgtc attaaataga tattacgaat attattcaaa taacaacaaa
    15961 gtggctggca caaaaaaaat aaataaaata aattctatta ctggtcaatc attaaatttt
    16021 tcatataaca aaagaaaggt attaagcaat ataagttttt caactgaaat gaataaacta
    16081 ttcatgatat caggagaaaa tggaacaggg aaaacaacac tgatgaaatt atttactaaa
    16141 cagttgccgg ttagtaataa tcaattattc gtcaacggga tagatattaa tatgattgat
    16201 aacgaatcat taagaaggtt aatttcaatc gcaacacaaa taccatatat aattaatagc
    16261 tccgtgaagg agaatatttt aatggggact aaaaactatg ataaaaaaaa attcagtaaa
    16321 ttaatagaac tattgggaat aagtaaattt atgacgaaat taggtattac tttggacgac
    16381 aatatttatg atgaaaaaat attattatct ggaggagaaa aacaaaaaat aagtttagct
    16441 agggcgctta ttaaatcagc ttcagttatt atattagatg aaccatttag aaatttagat
    16501 aatgaaactc gcgataattt gagggattat atactaaata caaaaaaaga agtttgttat
    16561 ataattataa ctcatgatca cattctagat aattttttgg attcaaatga tgaaatatta
    16621 tatttaaata aggagtatga taataatgaa tagcaaaaaa cttcagatgg gtgaaattgt
    16681 tagaggtcct agtcaaatat ctattgatgt tacatataaa tgtaatttta gatgtaaaca
    16741 ttgttacaat gcgagtggtg gagacaattg ttttatggat aatgagttga ctgatgatga
    16801 actgttagaa cttacaaaag atatatgtaa tttaaagcca tataatattt gtttctgtgg
    16861 aggagaaact ttactgagaa aaaaaatagt tttatcttgt gccactttat taaaagaggc
    16921 aggtattcct aatattgcta ttgttactaa tggatcatta gtaactcagg atgttgcaaa
    16981 agaattattc aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag
    17041 tagccatgaa gcacttagat tatatccaaa cagttttgaa aaggcaatta atgctatcaa
    17101 attatttaga gaattgtccg aagaagtgga tgttgctttt tgtcctacta gttttaatta
    17161 caatcaatta cgagaagtat atcaactagt aaatactctt aaagtgaagt ctttaagaat
    17221 tcaaccattt atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc caactgtttc
    17281 tcaatataga aaactagttt ctgatattaa ctatattaaa agaaatgaag attataccaa
    17341 gatagattgg ggagatccaa ttgatcatat catccgacac agaactttat tgaaagattt
    17401 ttcgaatttt gtttctatta ggtcagacgg aggattaatg gcatcacctt acttgccaat
    17461 tcaagttgga aatattaaat gtcacagtct ttctaaatat tggcgagcag gactttataa
    17521 agttttttct ttagatttag ttcaagatat agctactcaa gtttcttcaa tacaagatat
    17581 gagtaaagaa ttaaaaaaag gacatattaa atggcttaat gaagatatag aaattgatat
    17641 tctcgatgaa tacaaacatg atattttaat ttaggtttat cattggaaag tttagataaa
    17701 caaagtactt ttagaaagga gggaaaaaaa tgatttctga acctcaaatg aataatatgt
    17761 atgaaagccc aactataatg ggagctggtg aagctgcgcc aagagcttgt tcagcaatta
    17821 tggtgtgtgt gttattagca tctgcaggag taggtatcca agcaggagcg gtgtatactt
    17881 atgctgtagt tgctacagta gctgtagctg cagccgcagt ttggaaaaca gtaggattag
    17941 ttggttagga attaatagga gactaactgt ctcctattga ttccaatgat taagctaagg
    18001 aggaatttta agaaaataat gaaatacaat aatttaaaac cgaaattact taatgagaac
    18061 atagaatata taagagaaga aaattatgga tatttaacca tagttcccaa aaatggaact
    18121 gaattgaaag ataacgtttt caatccaaca gtaagatata tattggctaa ctgtactgga
    18181 aaaaacagtg taaaaaaaat agcgaatggt ttaaaaaaga agtttcctag tttagaatat
    18241 gaaaaattct atcaagatgt tgtaggaact attaaatttt tcaaacacta taatattatt
    18301 gatataggag attatagtat ggaagaaaag aaaatatata aaattgaaca aaatttagaa
    18361 ttagaattac taaatgaatc acattatgaa gaaatttata attttatcac agatcatcct
    18421 gaaagtatgt attttcatga agatatttat catcaaggaa atagtaaaga attagaagtt
    18481 gtccttagat ataatttatt ttcgtttacg agtgaatttt actttttaag acataaagag
    18541 agaattgtag gaatggtttc tataattgtt aatccatttc aaaacttatc ctacagtata
    18601 gtaaattacc tcatatgtga gcctgcatat actacgacta ttttgacaac agtattagaa
    18661 aatattaacg aagtttcaat tttagaagta aagaagctag tgattcattt gctaaattca
    18721 acagaagatg aatcactaat cccctattta aaagattcac atttttttaa agaagcggta
    18781 ctatctaaag aatatagaga gcatgatgtt ttgagatacg cttacattta ttaaataaaa
    18841 tttctgatag tattttaata attcggatca tatcataaga tataggaggg aaaagaagtg
    18901 attttattga ttttgtctct cgtattacta gttgtatcta atctaatgaa aaataatcct
    18961 cctacaaaaa ttaattcttt ttatggcttt agaactaaaa agtcaatgaa aaatgaaaaa
    19021 agttggcatt tagcacaaaa attgttgggg gatttttcaa aaaaaaaagt tatcccagta
    19081 tttttgatgg gtattgtttt tttcagtata gagatttata gtttagctat tttaaaaaat
    19141 gaaaattttt tcgttagtgt attaatgggt gaaagtttaa tcctaatact gattttgtgt
    19201 ttaattgttc atgaagtgaa taaacaattg tgaattaaaa aaataaaaac ctagtataat
    19261 cactttaata aattgttggt agaaggtgat caaaatggga gtaaacgaaa tgatgaaagt
    19321 cagattgagt ttttaaatga taccttcctc agaacttcaa aacgaattca aagattcgtt
    19381 gacaaatctt gagcaagatc atttaaaaaa atattttctc ttcatttgac gaaaaatgtt
    19441 tagaaatctt atataagtga agataaagta attggtgtaa ttaattgtag agaatagcct
    19501 ttgagcaatt gaagcgatac aattatttag aaaatgaact tcctccagca tactacatga
    19561 ttccttacgg gacgatgtta aagaagaaag caagaaattt tagaagtatt agtagataaa
    19621 ctaatgggct tttctcaatt taagggagta gcccaaatgt ttatcaaata tggtgcagat
    19681 acagtagcaa ccatgtttac agaagaatat caagtagact attatgaagt aatagataaa
    19741 tgtgtacatt ttgtttatga aaaagtgtgg aattttatga attttgcgat aagtgaagaa
    19801 atcttacgag tacaagaaaa cagaacagga atatttacca gagacagaaa gcttaatttg
    19861 attgcgcatt atatacggaa tcgctggtca tcaataccaa attttaatta taaaaaataa
    19921 ttatgaacaa tcttttatac aatatacaca ataaatcaag tagtatttat gaaaaaagac
    19981 ttcaaaaaca gaatgatttt ctatcagtct tagaaattat agagctaaaa attttggtca
    20041 cgcgtagaaa cgcgtttttt ttgttttttt gatagtaaag aacgtacaaa acaatgagaa
    20101 tttaaaagat tgtactacca gtttttttac cttttcattt gagtttagag taagtaaaat
    20161 attactataa aaacacatat tcttgaagaa atcttttttt tgctgtattc atttagatta
    20221 tgatagagaa aatacgtgag agggggacct gggatgtggc tatctgatga aaaaattcag
    20281 tggcgtagct atcagaagcg tatattgact aactttgcta cttatttaaa taacaattca
    20341 gtccatctag tggctccacc aggggctggc aagacctttt taggaattga gatgatccga
    20401 cgtttggatt gtaaaacctt aattttggtt ccttccctaa tattgaagaa acagtggtta
    20461 acaacaatcg aggaatattt cttagtaaat ccagcagata gttgctatct ttctggggcg
    20521 attgaacatc ctagtcatat tacagtggaa acgtatcaga ccatttacaa ccgactgaag
    20581 gaggagccaa cttatggtga tcagctggag ctgttagttt tttgatgaag ctcatcattt
    20641 aaaaaaaagc tggggtgaat tgttgattga cttgaagaaa aaacaaccga atctgttgac
    20701 gatttccttg acagccacgc ctccgattga tagctctcag acggagtgga agcaatatat
    20761 tgagttaaaa tggcctgatt gacgaagaaa ttagcattcc cgagttggta aaggaggagg
    20821 ttctatgtcc tcatcaggat tttctatatt tgattccggc taatcaggaa actactcagc
    20881 attattttac ctttttagag aaacaaaatg agctggtgga agcgttaatg gaaagccaag
    20941 agacggtgga ttatttgctt tctttagaat ttgttcagca tccgcttgag cattcgaagg
    21001 aaatttatga aaattttgag ctgttcacta gtgcattaat ttacttgaat catcataact
    21061 atgaattgat acccgaacat tggcagctat taggcagttc taagcagcaa ggtaaaaaag
    21121 cttcagttcc atctttaggt aaaaaagagc tgatcactat ttatcagcag atgtttgaag
    21181 atcaaccgga attgcctatt ttttatcaat tgaaaaaagc tggatgattg tttgatgagt
    21241 cgctgcattt gtttccagag tatccagaca ctgttggtag tcaggcgatt cctgagaaaa
    21301 agcaagcagt ttgtcaaatt ttgttaaaag aagaagttca gctgaagcaa gaactgacag
    21361 cggtcatttt gatggatcgt atttgcaagg aggtattagc aggcagtgat ggtaccttag
    21421 cttatggcgt ggttcctttg tttgatgaag ttgttcgtct attaggtaac actcagcaaa
    21481 tcggtgccat ctgtggagag ttcttactgt taccgaaaat gctagtaact gttgagctgg
    21541 cagaactagc gacgggtatg aatgaacatc cttatatgtc gggttatttt tatttgacgg
    21601 tggatgacaa aaaccgccaa gcattgatgc aagcagttac tcgcctatta accgaaaaga
    21661 agttgaatat tttggtagga accgttagtt tgctaggaga aggctgggat tgtcctgcaa
    21721 ttaatctgtt gatcttggct aatcaagctt ccacttttgt gcaaacacaa caactacgag
    21781 ggcggggctt acgcctaggt gcacaaaata aagtaacgaa catttggcat ttagcgattg
    21841 gattaccggg agttcctctg tctgaacagc ctgatttgaa aagaatgcta cggcgtttgg
    21901 gcaatatttc aggtctgacc tttgaatctt caccaaaaat tgagagtggt ggcgatcgct
    21961 ttgctttacc actggaatat acgaccacca cgatggaaac ttatactcag tcgatgttct
    22021 atttttcttg tgaacgaagt cggttgaaag aacaatggag tactgcttta gctgatggct
    22081 ggcacctatc tatgccaatc gtcgttagga aagtcgatca ggtaaagcaa aaacaagaaa
    22141 cttcattgcc gatagtagta aaaaatatag agcctctaag cttctggtca gctatattta
    22201 aaggtaactt gctggaatat ttccaaattc gtaggcgaca aaagctatgg gaaaaggaat
    22261 gtgcccgcaa aaaaagcttg gccatatgcg ttcaaacgac gttgcagcgc cttggtgaag
    22321 ttagtgatag aaaagtaatc gtagcggctg atcagcagga atttagatgc tatcttgaga
    22381 atgctggcta tcgagaagga cagcttttta ctgaagctat gagggagctt ttagcaccgc
    22441 tggagaatcc gcggtattta atcgaaggga aaaagatagt tctgacggta ccagaacgct
    22501 ttggccgaaa taagcaaatc gctaaccttt ttctaaacga attaaatcaa tcgtcgctgc
    22561 ccaaatgtca gttggtgtat actagaaatc ctgaaggacg ccaaaagcta gtgacttatc
    22621 gaatgaggca agtcttccag acccgcgatg aagtaattat tcaaaaaaac atttggacgt
    22681 aagttgatga aaataaattt tgctagagta gaacaagtcc gagaattaag ttgctactta
    22741 actgataatg ccaaggacag gagaatcttt tcttgacgac tgaaagaaac tatttgactt
    22801 atataacggt gtgtgatgaa tatttgcaag gcattctctt aaaaagtact cgtaatagtt
    22861 tttttgaaaa caaaacagta ctttattaat aggactgaca gagcgatata agaacatctg
    22921 cagacagagg tgaatgcttt tctcatgatc gatgaaacaa tggtacttcg gatattcttg
    22981 cttttagttt gaaccgttgt gattttcttt gattcctaaa agtagtttta tagaatgaaa
    23041 atcaagcata cggaatgctc ctttgattgt gttgtggtga ttcaatctaa tggagcttct
    23101 atatgttttt ttatttgcaa aaagaaccga tagaatattt ttatcagtct tagaaattat
    23161 agagccaaga aatttcaagc acacataaaa acgcgtgttt tatgcatttt atgagcaaaa
    23221 ataactaaag accattacgt aaaacatagg taaatcagca ttcaatctca aattatgtga
    23281 tcaaatgttg atattatttg attataagtt ttcttttgct attatcagtc tgcaaataaa
    23341 cagattatag agtatgaatg tagatagttc aatctttgtt tacgacctca catgtttttt
    23401 tactcaagag aaagtaatat aaagtgacta tatttgacag aactttacat aaaaaaagta
    23461 gttagttttc tgtagtaatc agaaatgtaa ataaattaaa aaaaagaaat ttagcagtta
    23521 tatattttta aaatattttg ggtgagtagt caatacaaca ttttgttcat caagcattaa
    23581 atgaagatgc aaggatgtta agaagttagc ttcttcaggg tcttgcatgg tcaaggcttt
    23641 aaatagttga tgatgttgtt ccataatttt ttcccatttt aatccttccg tttttactcg
    23701 taaccaacga aatcgttcca agtgcgtatt attatcttcc aaccattgcc atacttcttc
    23761 ccgttctgca atttcaaaac aagctttatg aaataaatta tcattatgaa aaaaactacg
    23821 agcattcttt tgactaacag ctttttcttg ttctgcaatt atagtttcta aaacttgttt
    23881 cttttgatta ttcatcttgg cacaacactc tagcataatt tgtcgttcta aatgttctct
    23941 gacaaaacga gcattttttg ctaaatctaa gtcgattaat gagatataat ttccactttg
    24001 aggaattgta tcaacaagtc cttgatgacg gagttgaatc aatgcttcac gtattggagt
    24061 acgaccgata gaaagcgttt cttccaattt tttttctgat actttttttc caggttctaa
    24121 gtcagcgtag ataatttgct gacgaataga ttgataagct tgattttgga gactagtttt
    24181 aattgtcata tttaatccct ctcaaaattt catttttgaa tttgatattt tagttctatg
    24241 ctcgttatat aatatcaaat ttcaaagtaa catactagct attaaaaaaa caaaaaatac
    24301 tcaaataaga tcaaagagaa attaagcttt aatttaagtt agttctaaaa aatatatatt
    24361 ggtgtgcttt tactaaaaaa agacacaata attctgacat ggtagaaaaa agaatatttt
    24421 tagttttttc ttattgacag cgattacatt cgattttata ctacaattgt aacggaaact
    24481 aacatatcag tatggagggg taaatatggt tttgattaaa gactggcaag attctatcga
    24541 aggattgaac aaaatgcaag ttcaactacc gtattttaat gtcgaagcgg tcaaacaaga
    24601 aggtcagaaa catccagtgt ggcttcattt tggtggagga aatctttatc gaggatttca
    24661 tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaatccggag tagttgtgtg
    24721 tgagacattt gatgaagaag tagtagaaaa agcttatcat gactacaaca atgatatttt
    24781 ggagattgtt atgggtgaag atggtgaact caaaaaaaga cttttagcag taacagctga
    24841 tagtttatat tgcaatccaa agaatataac ggactatcaa agagtggtta aatattttga
    24901 agatcctaca ttacagttta caacgtttac tattactgaa aaaggataca gtttaaaaga
    24961 ttctaaagga caattttttt cggtagtgga agcagatata aaatctggac ctaccaatcc
    25021 tcaacatacg atgagtattg tgacatcctt gttgtatagc aggtttaatg ctggagagtt
    25081 accgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt ttcaagatag
    25141 catttttatg attgcagaag cttggcaaaa aaaaggatac gtttctgaaa aatttattgc
    25201 ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgata gaattacgcc
    25261 aaatccttcg gaaagtgtca aaatggattt attgaaggcg ggtttcgaag acttagatat
    25321 aattcatact gcgaaaaata ccaatatcgc tgcttttacc aatactgaaa aagtccatta
    25381 tctagtcatt gaagatagtt ttccaaatgg tcgacctaaa ctggaagcgg ctggtgtgat
    25441 tatgacagat agagaaacag ttgataaagc agatatcatg aaggttacga catgtttaaa
    25501 tccgctccat acggcattag caataacagg ttgtttgttg gggtataaat caattcaggc
    25561 agaaatgcaa gatgatgatt tggttggctt aattaaaggg ataggttatg tagaaggctt
    25621 accagtagtt gaaagtccag gcgttattga tccaaaacaa tttattgatg aagtaatcaa
    25681 taaacgtttg cctaatccct atattccaga tacaccgcaa cgaattgcaa cagatacttc
    25741 tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa aagacggaaa
    25801 tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt atttattggc
    25861 agtcgatgat caaggaaaaa attttgtgcc aagtcctgat cctttattgg tagagttgca
    25921 ggaaaaattc tcagtattga aattaggtga acaagatcct gcaaagattc attgtgcggt
    25981 acaggaaatc ttagaaaata aggaaatatt tggtttagat ttatatcaag ttgagatagg
    26041 aaagaaaatt gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag ttcgtaaaac
    26101 aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac ttttagatgg
    26161 tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc agcaatgaaa
    26221 ggaattgttg gaacattaat ggatatacca gccggagagg tctggccgag agaacgaatt
    26281 aaagctttaa aagatgaagt cgaagccaat ggccttacct tgaaagttat tgagagtgtt
    26341 aatgttcatg atgatatcaa aattggtttg ccaacacgag atcaatacat cgagaattat
    26401 aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta taattttatg
    26461 ccagtctttg attgggtaaa atcagatttg gattatcatt tagatgatgg gtcttctacg
    26521 ttagcctata tttcaaaaaa tattcctact gatccagagg aaattattag aaaagtagaa
    26581 aattctacta ataattttga attgccgggt tgggaacctc atcggttatc acaaatcaag
    26641 caattattag aagcctataa agaagttgat gaagcgaaat tgcgagagaa ctttgcatat
    26701 tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc agttcatcca
    26761 gatgatccac catattctat tttcggatta cctcgtattg ttaaaaatcg tgacgattta
    26821 gaatggttgt gtaatgtagt ggattctcct tctaatggta ttacgttatg tactggaagt
    26881 attgcagaac gaccagataa tgatgtctat gaaattctat ctgaattttg tcaacgggat
    26941 cggattcctt ttgcacatgt tcgtaatatc aaatatttaa gtgataatgg actcgatttt
    27001 tatgaagcgc cgcataaatc agaatatggt tctctagata tgtataagat attaaaaaca
    27061 atgtacgata atggatttaa tggctatatt cgtccagatc atggtcggat gatttgggga
    27121 gaaaccggta gacctggtta tgggttatat gaccgagctc taggtgcagt ttatttaaat
    27181 ggtatttggg aagcgcttga aaagggggcg agtaaataat tatataatgg agtctattta
    27241 gaagaaaaat tttctcttct aaaaactaag ggaaggaaag gagtgcttaa atatgttttt
    27301 agataagaac tttttattag agaatgaaat agctaaaaga ctttttcata aatttgcaga
    27361 acaccaacca atcatcgatt atcattgtca tttagaccca gcatgtttgc tagaaaataa
    27421 gcaatacaag aatttgactc aggtatggtt aaatgataaa ggagctggag atcattataa
    27481 atggcgattg atgcgagcta atgggataga agaagagttg atcagtggtg caggtaatga
    27541 ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag gcgccaggta atcctatttt
    27601 tgaatggtct catttggaat tacaacgtta ctttgatatt gatttgacaa tttctgagaa
    27661 gaatgcacca gaaatttggc gacgtgccaa cgaaaaaatc caaacggatt cctatcgccc
    27721 aaaagaattg ctgaagatga tggatgtgaa agccatttgt acaacggatg atcctacaga
    27781 tgacttgcga tatcatactg agctttctaa acaggataat tctttcaagg tattacctac
    27841 tttccgtcca gataaggtct ggagtatcaa atcatcagga ttttcgaact atattttaaa
    27901 acttagtcaa gcagcgggta taccaatcaa aaactttaat gggttgaagc aggcattgag
    27961 ccaacgagtg gattattttc atcaaattgg ctgtcgatta gcagatcacg gtttgaatac
    28021 ttatttttat gaaatagcag atgaaaaaat actcgacaat ctgttaataa aagctttagc
    28081 aggaaaaacg gatttccatt tatcagaaat tgcacaattt gttacaggta ttcaattaca
    28141 tttgatgtct gagtatacaa gatatggttg gacgatgcaa atgcatttaa atgctttaag
    28201 aaataattca agtatattaa aggaacaaat tggagtagat gtcggtggtg attcgatggg
    28261 ggatcaagct agtttgacac aaaatgttgt agcattgttc gacactgctg aaaagagtgg
    28321 gtgcttacca aaaacaattt tatattcatt aaatccaacc gattggcttc cactagcaac
    28381 agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc caattgggat gtgcttggtg
    28441 gttcaatgat acagctgaag gaatgcgtca acagttgact acgatggcgc aacaaagttt
    28501 gttgtcgaat tttacaggta tgttgactga ttctcgaagt tttctaagct atcctcgcca
    28561 tgagtatttc cgtcgagtat tatgtcaatt actaagcgag tgggttaaag caggtcgaat
    28621 tccagatgat gaaatgtatc ttagtcagat agtggagaat atttgttatc gtaatgcgta
    28681 taattatttt aatttttttg aaatcgaaag tagaaaataa tacaaaaaat aaaagcttaa
    28741 atttatttat tgtaagttag tacaactagc tagagtatgt atccataaaa gaaggagata
    28801 gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg ctggagtcat tgcggttgta
    28861 cgtggaaaaa ataaagatga ggccattaaa gcaataaatg caattactgc gggtggaatt
    28921 aaagggattg aactaacttt tacagttccg aatgctgaag aaataatcaa agaaattgct
    28981 aaaaaatatg ctgatcaaaa agatgtaata attggagccg gtacagtttt agatgcgttt
    29041 actgctcgta ttgcaattat ggctggagca gaatatatcg taagtccatc ttttgatcga
    29101 caaacagcag agatttgtaa tttatatcaa attccttatt taccaggttg tatgacaatt
    29161 actgagataa aggaggcact taccgctgga gtagaaatca ttaaactatt tccaggttct
    29221 gcttttggtc catctgttgt aaaaacgtat caagctccat taccatatgt aaatataatg
    29281 cctactggcg gagtaagtct agataactta gaagagtggt ttgaagcagg cgttgttgca
    29341 gttggtgtag gtgggaattt gttggctcct gttgaaaaag gtgattttga agaagtaact
    29401 cgaatagcta gtaaatatat agataagttt tatgagataa agaattatta ggagaaaaaa
    29461 taaataatac tataaaatgt aaaaaataaa tactaactct ttatacttca gagtatagta
    29521 cgtaagttgg cagttcaaca gaatgaaaac gcttaacaat aggaaggagc gcaaattata
    29581 tggaaagaaa atttggtcta agagacaaaa taggatatat gtttggagat tttgggaatg
    29641 atttcacttt catttttgct agttcttttt taatgatttt ttatacaaaa gtattgggga
    29701 taagcggagc taccgtagga acattatttt tagtagcaag atttattgat gcagttacag
    29761 acataacaat ggggcgtatt gtggatggat caaaaccatc taaaactggc aaatttaaac
    29821 cgtggatttt acggatgagt ggtccagtcg ctttagcaag ttttttaatg tatcaaacat
    29881 caatggcatc agcttctatg agtattaaag ttatatatat gtatgtaaca tatatactat
    29941 ggggaagtat tttttataca tctattaata ttccttacgg ttcaatggca tcagctatca
    30001 cttctgttgc agaagaaagg acagcgttat caacatttcg tagtatagga gctaccttag
    30061 caggtatgtt cattggcgtg ttaacaccaa tttttattta tacaagagat agttctggag
    30121 ctcaaattgt acgtggtgga tcaactttta caattgtggc aggagtattt tcagtgtgtg
    30181 cagttatttg ttacgtaata tgttataaat taactgtaga acgggttaaa agtgatgaat
    30241 cgcagttaaa agtttcattg ttgaaatctt taaaaggatt agctaaaaat agagcgttgc
    30301 tttctataat agctgcagct atttttttgt tgcttgctca gcttttaaca atgagtatga
    30361 acaattatgt atttccagat tattataata atgcacaggg aatagtttta atgaacttta
    30421 tcaatcctat tttgatgttg gtacttgttt cacctgtaag tttaattttc agtaaaaaat
    30481 acggtaaaaa agaattggcg tcagtaggga tgttcttctc tgctattgta tatttacttt
    30541 tatttttgat aaagcctagt aatatgtatg tattcttagt tcttacttct gttggatata
    30601 tggggttagg tatttttaat acagtcattt gggcgaatat tactgatgta atcgatgatc
    30661 aagaagtgaa atctggccaa agagaagatg gaactattta tgcagtatat tcttttgccc
    30721 gaaaaattgg acaagcttta gctggcggtg tcggaggttg gactctttct ataatagggt
    30781 atgattcgtt agcaaaagta caaagtggag aagttttaca aaagctgtat aactcttcaa
    30841 cgttgattcc agcagtttgt ttctttgttg taggattgat tttatttttc gtatatccat
    30901 tatctaaaga aaaagtatta gaaaattata atttgctaaa tcaaaaacat aactaggaga
    30961 aaaaacatga acagttggat attttgtatt gattcagatg gttgtgtcat ggatacaatg
    31021 acttataaac acaatctctt tttcgggcca attgctgcag agatttttga tgttcatcat
    31081 agggaaaaat tcctacagga ttggaataat attaatttat attcagagac tcgtggaata
    31141 aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaccatc gattaatcga
    31201 ttaaagcact gggtatctgt aacaaaagaa ttgtctaatc catctttgaa gagggaaata
    31261 gaatactctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcga
    31321 cagattagtc aagctaaagg attagataaa ccctttttaa gtgctagaga tactataaaa
    31381 gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga agctattcaa
    31441 gaagaatggg aaagacatga attattatcc ttagttgatg aactgtgttg tcaagataaa
    31501 ggaaaaaaag aagacattat tcgaagtgta attgaaaatg gttgtgattt ggataagata
    31561 ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataagaataa agttttcttt
    31621 tatcctattt tggttaataa agaaaaagaa tcatgggaaa acttaagaac tgatatttta
    31681 tttaaatgtt tatcgggaga gtatatagat attgaacaaa aatatataga tactttttgg
    31741 aaaaatctta ctaataagta gaggagaaag aaaatgacta taaaagtaga cttacgaaaa
    31801 aaaccatatc atttagatga agatgctatt tcttggattg aagagacgat tgatgctatg
    31861 actttagaag aaaaaatagg tcaattattt gttaatatgg gatctagtcg tgataaaaat
    31921 tacttaagta gtatggtcaa tcagtaccat attggagctg tacgatataa tccaggaact
    31981 gctgatgagg tatatgatca aaactatatc ctacaaacag aaagcaaaat tcctttatta
    32041 attgctgcta atacagaagc aggtggtaac ggagcttgta cagatggtac cgaagttggt
    32101 gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt agggagagtt
    32161 tctggaattg aagcatcggc tattggatgt aactggagtt ttgctcctat tgtagatatt
    32221 aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaag ccctgataaa
    32281 gttttagaac ttagtttagc ttatatgaaa ggtattcaag agagtggaat tttgcctgca
    32341 gctaaacatt tccctggtga cggtattgac gaacgagatc aacatctatc ttttagtatt
    32401 aatagtttaa gtacagaaga atgggataat tcttttggaa aagtatactc aggcttaata
    32461 gaagctggat taccttcaat tatggcaggt catatccata tgccagctta ttcaaaacat
    32521 tttaatcttg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa agaattaatc
    32581 actgacttac ttagaggaaa actcgggttt aatggattag tagttactga tgctagtcat
    32641 atgttaggaa tgactggggc aatgaaacgg tcagaattat tgcccacttc tattgcggct
    32701 ggatgtgatc tattcctatt ttttaatgat ccagatgaag actttaatta tatgatggaa
    32761 ggttattaca aaggaattat ttcagaagag cgtttacgtg atgcattaag aagaatttta
    32821 ggcttaaagg ctaaactagg tttgcataaa aaagaaaaga ataaaatttt gttaccgaaa
    32881 caagaagcta tggaaaaaat tggtttaatt gaaaataaat caatttttaa agaggtagct
    32941 gatcaagcaa ttacactagt taaagataaa caagatattt ggcctattgt accagaaaaa
    33001 tataaaagga ttttactggt agatgtacat ggggtaaaag gtgggtttgg tgaagtagtt
    33061 gctggaaata gacctcaggc cattgattta ttgaaacaga ttttgtcagc aaaaggattt
    33121 gaagttgaaa aatacatttc acctgaagaa gaattgaaag gattatcgca agaagaaaaa
    33181 attaaaaata tagctggaat atatgctgct aagagaccaa tcaaagattt aactgaaaaa
    33241 tatgacttga ttattaatat agctaatgtt aacccaggta cagttcaaag aattgtttgg
    33301 ccagcaagta aaggtactcc ggatattcca ttttatattc atgaaattcc aacaattttt
    33361 gtatctgtac agtgtccgtt tcatttagct gatgtgcctc aagtaaaaac ttatatcaat
    33421 acttatgatg gaaaacaaga aactttagaa atattagtgg acaaactaat gggcctttct
    33481 gaatttaagg gaataagtcc aatagatgct tattgtgggt taaaggatac atttatttaa
    33541 tatttaaatt atataggagt gatttacatg ttatatccca ttatgacaga gacgaggcaa
    33601 gtaattgatt taagcggtct ttggagattt aagttagata caaatgatga cttacaagaa
    33661 gaacttgctt tagaaccaat ggttgaaaaa gagagttatc caatttcagt tccttcttct
    33721 tataatgatt tatttgaaac agagaacatt agaaatcatg ttggatgggt gtggtatgaa
    33781 aaagaagtga ttcttcctaa aaaattatta gaagaaagat tagttcttcg ttttggatct
    33841 gcaacacatg aagcaaaagt atttttgaat ggaaaattga ttaatcaaca caaaggaggg
    33901 tttacgccat ttgaatcaga aattaatcag tttataaaag atggaagtaa cagaattaca
    33961 gtggctgtaa acaatgttat tgacgatagc actttacctg tagggatttt gaaagaaacg
    34021 atacaggaag atggagacat aaaaaaagaa aatttggtta attttgactt tttcaattat
    34081 gcaggcatta atagacccgt taaattgtat acaactccga aagactatat aaaggatgtc
    34141 actattaata caaaaataat tgattcagat gcaaatgtag aatttaatat cgatacagaa
    34201 tggaccggag aaattctaat agcaatctat gacgaagaag ctcaacttat agcagaagct
    34261 aaggggaaag aaggagtgat tcaacttaag aacccaacac tttgggagcc tctaaatgct
    34321 tacttatata gtatgaaagt tagtttaatt aacgaaaata aagtgattga tacgtacaca
    34381 gaagaatttg gcattcgtag tattcaggtt tcgaatggga agtttttgat taataataaa
    34441 cctttttact ttaagggatt tgggaagcat gaggacacgt atattaatgg tcgtggtttg
    34501 aatgaagcag taaatatcaa agattttaat ttaatgaaat ggatcggtgc taattcattt
    34561 aggacttcac attatcctta ttcagaagaa attatgcgct tagcagatcg agaaggtatt
    34621 gtagttattg atgaagtacc tgcagtagga ttacatttga atttcatggc tactttatta
    34681 gatgacaacg tagaaaaaca tgatacttgg aaagaaatac ggacagaaaa tgctcacaaa
    34741 atggcgatta aggaattgat agaacgcgac aagaaccatc cgagtgtcgt aatgtggtct
    34801 attgcaaatg aaccagattc ggattctgaa ggcgctaaag aatattttga acctttagtt
    34861 gaattgacta aagaacttga ccctcagaag agacctgtaa caatcgttac ttacttaaag
    34921 tcaacaccag atatttgtaa agtaggagat attgtcgatg ttttatgttt aaatcgctat
    34981 tatggttggt atgtttcagg tggagaccta aaacatgcaa aatatctgtt gaaaaaagag
    35041 ctagatggat ggatggaaag atgtcccgat acgccaataa tgtttacaga atatggagca
    35101 gatacagtag ctggacttca tgatacagta ccaacaatgt ttacagaaga gtatcaagtg
    35161 ggttattacg aagcaaatca cgaagtaata gataaatgtg acaattttgt tggtgaacaa
    35221 gtgtggaatt ttgcagattt tgaaacaagt caagggattt taagagtgca aggaaataag
    35281 aaaggaatct ttactagaga tagaaagcct aaaatgattg ctcactatct aaagaaacga
    35341 tggtcatcaa taccaaattt cagttataaa aaataattta ccttttataa ggaatataaa
    35401 aaaggagtgg ctgcttaatt gccaactcct tttttttata aaaagatttt ctgtttaaag
    35461 aaggatattt ttttctagtt ttttcgaaaa aaaggcgctt tctgattgtt gtattagtgg
    35521 aaaggggaaa aaaggagcat atttatcatg caacaaaatt acaatgagaa atctatacaa
    35581 catcaatttg atgcctactg taaaaaagta ttacgaaacg aaactaagtc tattcgaaaa
    35641 cgaaacatta acataacaga taatgaggaa ccattgacga attcaaatga aggtaagtac
    35701 tcccaaactc attttgatga acaagatgtc tatttcttat ttggaatgga aatattaatt
    35761 tcagatcaaa agttatcggc agccattgat catttatcgg aaataagaag aaaggttatc
    35821 ctactttatt attttgcagg ttttaatgat actgaaatcg gtaaaatcct caatatgagt
    35881 acaagtggta tctggtatca acgaaagaag gcagttgaac aattaaaaat ggggtatgac
    35941 ttatggtaga caaccccaaa aaacggcagt atccattagt tcctctatcc attatccaat
    36001 ctgctctaga agaagatcca atttcaatca agatgatact caaacattat catagttata
    36061 tagctaagtt atgtttgaca aatggattta atgaggcggg gcaatttatt acctatgtag
    36121 atgagtatat gctaagacag ctagaaatta aattgataga agcgatctta aaatttaaaa
    36181 ttaactaact aaaaagtccc ctttccactt attctgttta aaagaatctt ctaatagtta
    36241 aaggattagt agattctttt aaacagaaaa cctattgatc attgaaattt aaatacattc
    36301 tatcagtacg ttaacacttc aaggagcata ttttagttgc gccatgattt cttactgtaa
    36361 agaaggagcg ataaacattc taaaataatt tttatagtca ataaaaagcg atgatttgaa
    36421 gttgggataa tgatacttcc gtaaggctcg ccgtaaactt ggagagaggt gaaattccta
    36481 tgtgcttttg acaaaagcag ctgattaaga attatttcca tatgttgatt tcaacatttt
    36541 ttctattatt tttaagacca aattcagtga ggcaaatagc atcatgtaaa tttagttttt
    36601 ctattgaacg ttctccactt tttagttttt ttatagtact caaatttaat cctgtatgtt
    36661 tagagatctc atttgcagta tggttttgta aagtttctcg gattattgtt atattagcaa
    36721 tttcattacg cataattatg ttcctttctt atcaaaaata tcaatgacta cagtgggctt
    36781 ttagtgaaaa aaaggggtac tacagtctct tttgaaaaca aaaaaataca gtatatacgt
    36841 tttctaaatg ctaagcacca acgtttacac tgtatttaag ggtttggcac ccaatgggct
    36901 gtgaggggct cgaacccgcg acccgatgat taagagtcat ctgctctacc aactgagcta
    36961 acagcccaaa ttaatacttt gttattctaa cacctaaaat aaaaaagtgc aatacttatt
    37021 atcagaattg agagtgaatt ttatggattt tcaagattat gaaaaaatat ttgaaagtta
    37081 tccatcgata ttgaaaatta ctgatttaac taaaatgtta aatattggaa aaaataaagc
    37141 ttataatttg ctgaaagata gatcaataga aaatataaaa attggagcaa ggtacaaaat
    37201 acctaaaaaa gctgtcattg agtatatatt gaaaatacaa gatgaaggta aaaatcatgc
    37261 tataataaaa tagtcaaaat ttttgccgat atttcagttt aagaggagag attgaattga
    37321 aattaagagc aaacataatt gaaaaaagag gaacattaca agtagttgta tcttataaag
    37381 actctaacgg aaaaattaga caaaagtgga aagatacaaa attaaaaaag aaaggaaata
    37441 aaaaacgagc agaaaaaatt agagatgaat ttttactaga attggaaagt caattagcta
    37501 tggaatgtga atgtgctaat ccaaatttat tgttttatga ctatttgctt aattatttgg
    37561 aagtatcaaa aaaacaagta gcatttaaca catatgttgg gtataagcat tacgtccata
    37621 atcgaattta caagtttttt tatcctaaaa aaattaaact aaataggtta aagccttatc
    37681 atttacaaga gttttatcaa tatatgttaa atgataactg tacagcaaat acggttattc
    37741 attatcacgc atttatacgc aaagctcttc aagaggctgt tattacagaa ttgataagta
    37801 caaatgtagc agataaagta agaaagccaa aaaagaaaca atttgtttct caagtttata
    37861 atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc tatttggtag
    37921 ttcttttaac agcattttat ggattgcgaa gaagtgaagt tttaggatta aaatggtctg
    37981 ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa aatccagaga
    38041 atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat cgtacattac
    38101 ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa caagataatc
    38161 gtattctttt aggagatgat tatattttag aagataatga gtacgtattt actatggaag
    38221 atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg attaagaaaa
    38281 atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca ataatgttag
    38341 atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg tattcgacaa
    38401 ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca gaagcattag
    38461 aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg ggtatcgctt
    38521 cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa tgaaaaaatt
    38581 cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat agagcatttg
    38641 gttaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat
    38701 agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta
    38761 cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac
    38821 caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt
    38881 tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc
    38941 ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga
    39001 ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga
    39061 tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc
    39121 ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg
    39181 atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg
    39241 taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag
    39301 aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa
    39361 tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat
    39421 tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc
    39481 atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt
    39541 tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa
    39601 aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat
    39661 gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat
    39721 aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg
    39781 ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag
    39841 caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc
    39901 gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta
    39961 ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg
    40021 ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga
    40081 acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt
    40141 ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa
    40201 gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg
    40261 gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat
    40321 ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa
    40381 gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga
    40441 ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt
    40501 gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca
    40561 ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg
    40621 gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta
    40681 gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc
    40741 tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa
    40801 cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt
    40861 gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt
    40921 gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac
    40981 cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa
    41041 ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct
    41101 tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc
    41161 ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt
    41221 tggggttatg attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt
    41281 gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt
    41341 tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg
    41401 atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat
    41461 tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga
    41521 tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga
    41581 taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac
    41641 tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga
    41701 tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac
    41761 gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc
    41821 gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga
    41881 acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc
    41941 ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg
    42001 tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt
    42061 gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat
    42121 tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca
    42181 tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat
    42241 ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact
    42301 tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt
    42361 acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc
    42421 tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac
    42481 agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact
    42541 tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa
    42601 agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat
    42661 tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca
    42721 attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat
    42781 ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg
    42841 ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat
    42901 caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt
    42961 gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt
    43021 cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg
    43081 attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa
    43141 ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg
    43201 gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta
    43261 agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta
    43321 gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt
    43381 cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat
    43441 ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta
    43501 gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg
    43561 gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat
    43621 gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa
    43681 ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt
    43741 tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa
    43801 atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg
    43861 aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc
    43921 cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg
    43981 aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca
    44041 gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc
    44101 agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata
    44161 ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt
    44221 ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa
    44281 gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag
    44341 ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg
    44401 caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat
    44461 aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat
    44521 taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg
    44581 tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat
    44641 cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt
    44701 tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca
    44761 ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac
    44821 ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca
    44881 gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt
    44941 aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa
    45001 gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct
    45061 ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat
    45121 atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg
    45181 tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc
    45241 tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa
    45301 acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca
    45361 aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta
    45421 caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt
    45481 atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt
    45541 ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg
    45601 acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc
    45661 gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa
    45721 gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg
    45781 aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg
    45841 gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc
    45901 ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa
    45961 agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt
    46021 attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg
    46081 atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa
    46141 gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat
    46201 gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca
    46261 gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt
    46321 ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat
    46381 gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc
    46441 catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata
    46501 agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg
    46561 tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata
    46621 gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa
    46681 taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat
    46741 caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc
    46801 aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat
    46861 aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc
    46921 tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc
    46981 aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc
    47041 gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt
    47101 gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat
    47161 gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc
    47221 cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct
    47281 gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg
    47341 attttctatg tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat
    47401 cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg
    47461 cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac
    47521 tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg
    47581 gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga
    47641 aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct
    47701 tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac
    47761 atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg
    47821 tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt
    47881 ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca
    47941 agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat
    48001 gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa
    48061 atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa
    48121 tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat
    48181 tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc
    48241 tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt
    48301 aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta
    48361 tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa
    48421 tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat
    48481 tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat actagccctt
    48541 tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg
    48601 atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt
    48661 agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc
    48721 cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact
    48781 cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca
    48841 tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta
    48901 acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa
    48961 caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct
    49021 taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga
    49081 tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa
    49141 ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc
    49201 ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata
    49261 atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa
    49321 agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac
    49381 agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc
    49441 attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct
    49501 gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa
    49561 aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac
    49621 gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc
    49681 atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg
    49741 aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg
    49801 gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg
    49861 tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag
    49921 aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat
    49981 caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa
    50041 tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg
    50101 ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg
    50161 attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac
    50221 atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat
    50281 caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact
    50341 tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata
    50401 tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa
    50461 tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc
    50521 caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg
    50581 gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag
    50641 taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg
    50701 cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag
    50761 aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt
    50821 ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta
    50881 aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat
    50941 tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag
    51001 tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca
    51061 agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg
    51121 aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg
    51181 ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac
    51241 gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg
    51301 ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg
    51361 gcggtatgat gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt
    51421 tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt
    51481 atcaattact tgttgtttgg ctctttttgt tacatggtta taaatagaca gagttgtgtt
    51541 tgcatcagaa tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa
    51601 tagtgaaacg tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc
    51661 ttcctctact ttatggagta tagcgttaaa tgagtgcagc gcaagcggag tgccacttgt
    51721 agatataaat atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat
    51781 cacactttca atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt
    51841 attttttgga gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc
    51901 attaatggaa attttcccat cctcatagtc cttcatttgc aaagctagca attccccata
    51961 tctcaaacca gttaaatata gaaactcagc tattatgccg tgtagttttc tgcgaggatt
    52021 ggagtatagc tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttattcat
    52081 agaaagcctt cttttttctt cttccacttt tttaggatgg attttaactg ctagcgcagg
    52141 gtttctttga atgtattttc tatcgattgc atagtttagc ataacagata gagttgtttt
    52201 tgtttgtttt gtgtagttca gtgagaggtc accaaacgta tacatatctt caattatctt
    52261 attaatgagt gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt gcttagaaac
    52321 atgtttcatc atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata
    52381 ttcataccat tctttataca gctcaccaaa agtgatatct gatttattgt aatcttcgag
    52441 tgcttcttta atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt
    52501 acttgtaagt gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc
    52561 aataaacttg aattggccat ctttggtctg ttcaacccac acttttaaca tctcctatca
    52621 tttgctataa taggcataac aaatagacct atataggttt gttttctaaa agcacgctct
    52681 tactctggac ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct
    52741 ttttcaaatg atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg
    52801 aaatgagcag ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg
    52861 gcatatgtct ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg
    52921 atatctgaat cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg
    52981 ttttcttcaa attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca
    53041 tcaccaactt ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa
    53101 gatgaagcag aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta
    53161 ctatctgatc caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc
    53221 caaaaccata cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc
    53281 ataacttttt ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca
    53341 gaaatgaggt atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt
    53401 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    53461 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    53521 tccataaatt tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata
    53581 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    53641 gtagaagtat cgtacaaaac tgaatattca aaatgagaag cggtgaaatg cgcaagttca
    53701 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
    53761 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
    53821 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
    53881 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    53941 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctc
    54001 tttcttctct acctagaagg tagtctacag agactccaaa gtaatcagct aaccgagcta
    54061 actcaataga attaggagtt tgtcttttcc aacttcctaa atatccattc gaatacccga
    54121 aattcatttc gagctgtcta atggataatc ctttttcttt tgctaactcc tttattattt
    54181 cgtaagtatt cattgataaa tcaacctttc tgaatgctta caaaaaaagt ttagataaat
    54241 aatgtaaaaa tagttgactt atatacatta ttaatctata ctatgttttg taaacaagtt
    54301 taacaactaa aaagacaaca aaaaatacta ttgattaata aatgccaacc gccaagaaag
    54361 ctttttaaat caaagtttat atgtcttatt taactatgca tttagtatag attattaatc
    54421 taataaagtc aacgcagttt tttaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    54481 ggagagaaga atatggaaaa aacagtctcg gcaaaaatcg aagatttgaa attagacata
    54541 ctgaaacaag caaaagtggc gatggaacac gcggtagata aagaagactc tgccatggtt
    54601 gcagccatag cagagatttt agctcacgtt tagtcatcgt ttttacggtc ttctggaagc
    54661 tgaccataga taacagcata atgaatatct aaataagctt gaacaatttc ttttggcgaa
    54721 attgattcgc ctttagtaga tacttgtgat tcgtgattta gccatgcaac aactaaatcc
    54781 gcagcgattt caaacggcgt tacactttgt gtcagttgcc ataaagaatt tcacaatatg
    54841 tatggtcgtg gtaataatac caaagagcaa tttgaggaat ttatggaaat taaaaaatac
    54901 aaaaaatcat ttattaaagg aggctaaaca atgaacaagg aaatgagcca aacaatcaag
    54961 gtacaaaaaa tgatagatga tctaacacat ggaattgata gccaagccga taaaatcatc
    55021 aaggaattgc aaggacaaaa agttaaggat gcaaaaatgc ttctcaagac tatcaacttt
    55081 gaaatgaatc caacaaaaag aaagctcgcc gatgtgttgg aagaaaagtt ggcttccgct
    55141 atcaacgagc aagaattact atttgaaacc gatactttta acagctgatg ttttatagat
    55201 gacagtttca tccgaatcta tatcatcgcc gagagaaaag aatgttgctt ttgacaaaaa
    55261 gccgataaga cctacaagcg gtcttgatgt tgcaagagta gataccccaa tatcaccttg
    55321 caaggtattc acgtaaattt gagaagaata gaacattcca tctttttctt cgatatttga
    55381 cgcatcatcg tatccaacga ccaaagtatc ggcaaatatt ttgatttttt ctccatcaag
    55441 catagtaatt acaaattctg gtttcataaa ttttcacctc cttaacaatt atttcagcct
    55501 gtcacactga taaggaaatt ataccaaaga aaggaatgag aaatatgaat acaccacaaa
    55561 tttttaattt cgagcaaaac gaagttcgaa ctattttagt aaatgatgaa ccatattttg
    55621 taggaaaaga tgtagcagac gttctaggat attcgaatcc gcaaaaagct attcgagatc
    55681 atgtggactt ggaggataag acgcagaacg attcgttcac cgtcaatgga acagcagttg
    55741 ttttgattaa cgagtcaggc ctttacagtt taatcttaaa atcaaaactt ccctctgcca
    55801 aaaaatttaa acgttgggta acgagtgaag tccttccaac aattagaaaa acaggtagct
    55861 attcaaacgt acctcaaagt tttgcacaag cattgcgttt agcagcggat ttagaagaaa
    55921 agaaccaatt actcgaacaa caaattgccg agtacgaacc aaagattagc tacttagata
    55981 cgattctttc atcgacagat acggtagcta cttctcaaat tgcagctgat tacggaatgt
    56041 cggcaattgc tctaaacaaa ttgctaaacg agttaggtgt tcagcataaa gttagcggac
    56101 aatggatact ttaccgaaaa catatgaacc aaggatacac aaaatcgcac acaagtgaga
    56161 taccgaaagc cgatggcggc actaaagttg taatgaatac caaatggaca cagaaagggc
    56221 gagtgtttat ttacaactta ttaatcgcag agggctatta ccctcaaatg gatttagagg
    56281 aaattggtta gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag
    56341 agaaaaaatc aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga
    56401 aagatttgga attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc
    56461 agaagcaaac agaattatag attcgattat cactatgtac gaattgtaga gggaggtaaa
    56521 gtcgttggaa gataaaatca ttcttacaaa atcagagctt caagaaatgt taaacatcga
    56581 gtatggaacg ataattttta ggagggataa taagtgaaaa aaccaacact ttcagagctg
    56641 atagaagctg ctgagaaggc agtaaaacca gacgactggt accgacaaag tttaatcttg
    56701 gagaagttcc acggcatgtc aaaaactact ttagttgaat actgcaagga aatggaaaca
    56761 attcctgaat ttgcagaagg aatacttcgt ccaggtcact caaccacatt tattcattac
    56821 catactttta tttggttttt aaagtggaaa gacgcaaaca aatatcgtgt aaaaagttta
    56881 tcgccttcag atgttttgaa ggaggcaagt taatgggcaa attcaacaga gcactagtat
    56941 tcagcgcacc gctaatcatc tacgctttag gcctctgggg aagcagacaa gcgttgatag
    57001 ggacaatcgt ttacatggtt tggattttta tggggcttga tgaagctgag cacagagcga
    57061 aaaaagccag tcgggaggga ctgactaaaa tatgattttt ttatttttgt tcctttgggc
    57121 tgctattgcg tacttcatcc agctccttgt tgtcaaagtt gctcttattt cttttaggaa
    57181 gaacaaaaga aaatagcgct ataaatgaaa gataagtagc cgctacatta aactgttctt
    57241 ttgaaaaagt tacagtcatc ggaatgataa ttgatattga ggaagtaatc ttaattaaaa
    57301 atatagcaat attttcaaca tcgcgttggt ttttatttaa cattgtccaa catgttttag
    57361 caacaataaa aataattaca catgatatta gcacacagac tgaaaaaagt tttagaaatg
    57421 cgatgtagtc agtgttattg aactccttca aaatactatt tttattcaat gtaataaaag
    57481 gaattttttt aattgaagac aggaaagata aggtaataaa cagaattata tatatggtta
    57541 aagtcgcagt aagaatgtaa ttgagctttt tcctcataga atcacctcac tttctaacga
    57601 aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag
    57661 aagctgtaac tacgagctta caagtggcag aaagtttcaa aaagaaacat aagcatgtac
    57721 tagaagcaat tgaatcaata aaaagatcgg tcgaaaattc ggccaatgtt gaagatgggt
    57781 ccaattttgg acagatgttt gtggaaggga acgagccaga ctcatacgga agaagtcgga
    57841 gagtttattt catgaataga gacggatttt ccttgctagc tatgggattc actggaagta
    57901 aagcaataaa tttcaaacta aaatttattg aagctttcaa cgaaatggaa gatgttattc
    57961 ggaagaatac tgttcctcaa acaattgaag acatgatgat ctatcaatta gaagaaatga
    58021 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
    58081 agtttgaaat taagcaaaaa ggaaatatga aagttatgga agttctaggg ggaaaagaaa
    58141 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
    58201 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
    58261 catttattga aatgtggtta ccagaaactg cgatccgcat ggaaatcgat caactgaaca
    58321 gacaacaaag acttttcggt gatgaaaatg aatagagctg aagcgctaag aatagggacg
    58381 gtaattgcta atcgctggtg gagacacaat aaaccaaaca tcctaagcca acaacatatc
    58441 gaaaagcaaa aagcatggca acaaataaaa aagtgactca gccggcaagc atagagtcac
    58501 aaaacatatc taaggagaat tttagcatat gaataaagaa ctttccactt tagatcaata
    58561 tttgactgat tctgaatggg gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag
    58621 acgaaatctt ttgacgaacg aagaactatc atgtgatcaa gatgacttgg gaaattttgt
    58681 gagtatttgg gatcatgttt accttatcca tctatcaaaa cattcaaata aacctgaata
    58741 catctatgtc atcgaagatg gcttgattga tgcgctagaa gagtacaaca gagataactt
    58801 gattgatatc tcttattacg ga
//
LOCUS       DACECS010000010        57128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.010, whole genome shotgun sequence.
ACCESSION   DACECS010000010 DACECS010000000
VERSION     DACECS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.010"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IWO31_000647"
     CDS             complement(<1..257)
                     /locus_tag="IWO31_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301087.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IWO31_000648"
     CDS             complement(273..923)
                     /locus_tag="IWO31_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301088.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IWO31_000649"
     CDS             complement(934..2106)
                     /locus_tag="IWO31_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0301089.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IWO31_000650"
     CDS             complement(2125..2997)
                     /locus_tag="IWO31_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0301090.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IWO31_000651"
     CDS             complement(3019..3939)
                     /locus_tag="IWO31_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301091.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IWO31_000652"
     CDS             complement(3939..4208)
                     /locus_tag="IWO31_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301092.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IWO31_000653"
     CDS             complement(4205..4585)
                     /locus_tag="IWO31_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAQ0301093.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="IWO31_000654"
     CDS             complement(5052..6515)
                     /locus_tag="IWO31_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0301094.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="IWO31_000655"
     CDS             complement(6579..9596)
                     /locus_tag="IWO31_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0301095.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="IWO31_000656"
     CDS             complement(9747..10586)
                     /locus_tag="IWO31_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0301096.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="IWO31_000657"
     CDS             10731..10976
                     /locus_tag="IWO31_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301097.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="IWO31_000658"
     CDS             11039..11461
                     /locus_tag="IWO31_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301098.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="IWO31_000659"
     CDS             complement(11555..11872)
                     /locus_tag="IWO31_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301099.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="IWO31_000660"
     CDS             complement(11899..12105)
                     /locus_tag="IWO31_000660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301100.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="IWO31_000661"
     CDS             complement(12086..12268)
                     /locus_tag="IWO31_000661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301101.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="IWO31_000662"
     CDS             complement(12282..12632)
                     /locus_tag="IWO31_000662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301102.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="IWO31_000663"
     CDS             complement(12658..12960)
                     /locus_tag="IWO31_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301103.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="IWO31_000664"
     CDS             complement(12963..13196)
                     /locus_tag="IWO31_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301104.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="IWO31_000665"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="IWO31_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="IWO31_000666"
     CDS             complement(14627..15742)
                     /locus_tag="IWO31_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301105.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="IWO31_000667"
     CDS             complement(15748..16146)
                     /locus_tag="IWO31_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301106.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17175..17561)
                     /locus_tag="IWO31_000668"
     CDS             complement(17175..17561)
                     /locus_tag="IWO31_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0301107.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17558..18079)
                     /locus_tag="IWO31_000669"
     CDS             complement(17558..18079)
                     /locus_tag="IWO31_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0301108.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWO31_000670"
     CDS             complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWO31_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0301109.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19148..20563)
                     /locus_tag="IWO31_000671"
     CDS             complement(19148..20563)
                     /locus_tag="IWO31_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0301110.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20532..22685)
                     /locus_tag="IWO31_000672"
     CDS             complement(20532..22685)
                     /locus_tag="IWO31_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0301111.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22943..23179
                     /locus_tag="IWO31_000673"
     CDS             22943..23179
                     /locus_tag="IWO31_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0301112.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23198..23413
                     /locus_tag="IWO31_000674"
     CDS             23198..23413
                     /locus_tag="IWO31_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0301113.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24116..24868)
                     /locus_tag="IWO31_000675"
     CDS             complement(24116..24868)
                     /locus_tag="IWO31_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301114.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24883..26130)
                     /locus_tag="IWO31_000676"
     CDS             complement(24883..26130)
                     /locus_tag="IWO31_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0301115.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26206..26352)
                     /locus_tag="IWO31_000677"
     CDS             complement(26206..26352)
                     /locus_tag="IWO31_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0301116.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(26456..26767)
                     /locus_tag="IWO31_000678"
     CDS             complement(26456..26767)
                     /locus_tag="IWO31_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0301117.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(26769..26966)
                     /locus_tag="IWO31_000679"
     CDS             complement(26769..26966)
                     /locus_tag="IWO31_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0301118.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(27360..29087)
                     /locus_tag="IWO31_000680"
     CDS             complement(27360..29087)
                     /locus_tag="IWO31_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0301119.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(29080..30273)
                     /locus_tag="IWO31_000681"
     CDS             complement(29080..30273)
                     /locus_tag="IWO31_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0301120.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(30460..31104)
                     /locus_tag="IWO31_000682"
     CDS             complement(30460..31104)
                     /locus_tag="IWO31_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0301121.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(31198..31332)
                     /locus_tag="IWO31_000683"
     CDS             complement(31198..31332)
                     /locus_tag="IWO31_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0301122.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWO31_000684"
     CDS             complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWO31_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0301123.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(33463..33936)
                     /locus_tag="IWO31_000685"
     CDS             complement(33463..33936)
                     /locus_tag="IWO31_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0301124.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            34337..34561
                     /gene="nrdH"
                     /locus_tag="IWO31_000686"
     CDS             34337..34561
                     /gene="nrdH"
                     /locus_tag="IWO31_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0301125.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            34720..36879
                     /gene="nrdE"
                     /locus_tag="IWO31_000687"
     CDS             34720..36879
                     /gene="nrdE"
                     /locus_tag="IWO31_000687"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0301126.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            36929..37894
                     /gene="nrdF"
                     /locus_tag="IWO31_000688"
     CDS             36929..37894
                     /gene="nrdF"
                     /locus_tag="IWO31_000688"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0301127.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(37983..39122)
                     /locus_tag="IWO31_000689"
     CDS             complement(37983..39122)
                     /locus_tag="IWO31_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0301128.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(39431..40144)
                     /locus_tag="IWO31_000690"
     CDS             complement(39431..40144)
                     /locus_tag="IWO31_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0301129.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWO31_000691"
     CDS             complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWO31_000691"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0301130.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            41333..42865
                     /locus_tag="IWO31_000692"
     CDS             41333..42865
                     /locus_tag="IWO31_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0301131.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(43056..44258)
                     /locus_tag="IWO31_000693"
     CDS             complement(43056..44258)
                     /locus_tag="IWO31_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301132.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(44340..45029)
                     /locus_tag="IWO31_000694"
     CDS             complement(44340..45029)
                     /locus_tag="IWO31_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0301133.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWO31_000695"
     CDS             complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWO31_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0301134.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(45556..46059)
                     /locus_tag="IWO31_000696"
     CDS             complement(45556..46059)
                     /locus_tag="IWO31_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0301135.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWO31_000697"
     CDS             complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWO31_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0301136.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWO31_000698"
     CDS             complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWO31_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0301137.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWO31_000699"
     CDS             48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWO31_000699"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0301138.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWO31_000700"
     CDS             48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWO31_000700"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0301139.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(49671..50690)
                     /locus_tag="IWO31_000701"
     CDS             complement(49671..50690)
                     /locus_tag="IWO31_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0301140.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(50863..51750)
                     /locus_tag="IWO31_000702"
     CDS             complement(50863..51750)
                     /locus_tag="IWO31_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0301141.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWO31_000703"
     CDS             complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWO31_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0301142.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWO31_000704"
     CDS             complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWO31_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0301143.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWO31_000705"
     CDS             complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWO31_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0301144.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            52981..53625
                     /locus_tag="IWO31_000706"
     CDS             52981..53625
                     /locus_tag="IWO31_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0301145.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(53681..54184)
                     /locus_tag="IWO31_000707"
     CDS             complement(53681..54184)
                     /locus_tag="IWO31_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0301146.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(54197..54243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            54781..55746
                     /locus_tag="IWO31_000708"
     CDS             54781..55746
                     /locus_tag="IWO31_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0301147.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(55845..57017)
                     /locus_tag="IWO31_000709"
     CDS             complement(55845..57017)
                     /locus_tag="IWO31_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301148.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(57004..>57128)
                     /locus_tag="IWO31_000710"
     CDS             complement(57004..>57128)
                     /locus_tag="IWO31_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301149.1"
                     /translation="LYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        17012 a        11484 c         9386 g        19246 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 atttatcata ggataaagag tgatagttta atacttaaag agacatatta attttattta
    16621 aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    16681 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    16741 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    16801 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    16861 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    16921 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    16981 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    17041 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    17101 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    17161 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    17221 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    17281 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    17341 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    17401 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    17461 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    17521 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    17581 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    17641 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    17701 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    17761 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    17821 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    17881 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    17941 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    18001 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    18061 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    18121 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    18181 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    18241 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    18301 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    18361 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    18421 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    18481 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    18541 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    18601 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    18661 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    18721 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    18781 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    18841 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    18901 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    18961 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    19021 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    19081 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
    19141 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
    19201 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
    19261 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
    19321 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
    19381 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
    19441 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
    19501 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
    19561 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
    19621 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
    19681 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
    19741 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
    19801 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
    19861 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
    19921 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
    19981 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
    20041 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
    20101 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
    20161 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
    20221 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
    20281 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
    20341 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
    20401 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
    20461 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
    20521 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
    20581 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
    20641 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
    20701 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
    20761 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
    20821 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
    20881 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
    20941 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
    21001 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
    21061 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
    21121 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
    21181 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
    21241 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
    21301 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
    21361 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
    21421 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
    21481 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
    21541 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
    21601 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
    21661 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
    21721 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
    21781 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
    21841 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
    21901 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
    21961 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
    22021 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
    22081 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
    22141 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
    22201 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
    22261 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
    22321 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
    22381 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
    22441 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
    22501 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
    22561 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
    22621 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
    22681 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
    22741 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
    22801 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
    22861 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
    22921 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
    22981 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
    23041 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
    23101 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
    23161 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
    23221 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
    23281 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
    23341 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
    23401 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
    23461 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
    23521 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
    23581 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
    23641 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
    23701 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
    23761 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
    23821 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
    23881 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
    23941 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
    24001 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
    24061 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
    24121 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
    24181 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
    24241 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
    24301 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
    24361 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
    24421 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
    24481 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
    24541 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
    24601 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
    24661 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
    24721 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
    24781 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
    24841 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
    24901 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
    24961 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
    25021 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
    25081 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
    25141 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
    25201 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
    25261 tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa
    25321 ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta attcttttat
    25381 ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa
    25441 atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt
    25501 atattttttt agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg
    25561 cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat
    25621 taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa
    25681 taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc
    25741 aacaatccta tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga
    25801 atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat
    25861 atacgtcata accatggaag taataataga tctgaaatca tcaataagca tacataatag
    25921 taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc
    25981 gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat
    26041 caataaacat acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa
    26101 gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt
    26161 aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt
    26221 aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc
    26281 agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt
    26341 tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat
    26401 atacctattt ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa
    26461 attgagattt atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa
    26521 tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat
    26581 aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga tctaattcat
    26641 atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata tcagaatatt
    26701 taggatcttt acttatagat atatcattat ataattcatg aacaatttgc ttagcatttt
    26761 ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat agacattcct
    26821 gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt gcaatacact
    26881 ccatttccat aatattttcc actatgagtg gtacccccat agataagttg tgtctcttta
    26941 atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg tttttagact
    27001 attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt agcaaaataa
    27061 ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc tttaatattt
    27121 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
    27181 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
    27241 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
    27301 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
    27361 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
    27421 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
    27481 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
    27541 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
    27601 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
    27661 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
    27721 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
    27781 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
    27841 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
    27901 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
    27961 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
    28021 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
    28081 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
    28141 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
    28201 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
    28261 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
    28321 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
    28381 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
    28441 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
    28501 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
    28561 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
    28621 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
    28681 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
    28741 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
    28801 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
    28861 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
    28921 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
    28981 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
    29041 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
    29101 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
    29161 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
    29221 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
    29281 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
    29341 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
    29401 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
    29461 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
    29521 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
    29581 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
    29641 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
    29701 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
    29761 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
    29821 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
    29881 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
    29941 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
    30001 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
    30061 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
    30121 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
    30181 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
    30241 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
    30301 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
    30361 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
    30421 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
    30481 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
    30541 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
    30601 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
    30661 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
    30721 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
    30781 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
    30841 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
    30901 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
    30961 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
    31021 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
    31081 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
    31141 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
    31201 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
    31261 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
    31321 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
    31381 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
    31441 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
    31501 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
    31561 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
    31621 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
    31681 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
    31741 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
    31801 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
    31861 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
    31921 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
    31981 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
    32041 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
    32101 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
    32161 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
    32221 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
    32281 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
    32341 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
    32401 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
    32461 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
    32521 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
    32581 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
    32641 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
    32701 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
    32761 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
    32821 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
    32881 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
    32941 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
    33001 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
    33061 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
    33121 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
    33181 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
    33241 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
    33301 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
    33361 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
    33421 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
    33481 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
    33541 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
    33601 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
    33661 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
    33721 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
    33781 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
    33841 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
    33901 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
    33961 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
    34021 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
    34081 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
    34141 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
    34201 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
    34261 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
    34321 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
    34381 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
    34441 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
    34501 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
    34561 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
    34621 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
    34681 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
    34741 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
    34801 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
    34861 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
    34921 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
    34981 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
    35041 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
    35101 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
    35161 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
    35221 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
    35281 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
    35341 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
    35401 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
    35461 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
    35521 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
    35581 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
    35641 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
    35701 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
    35761 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
    35821 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
    35881 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
    35941 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
    36001 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
    36061 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
    36121 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
    36181 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
    36241 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
    36301 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
    36361 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
    36421 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
    36481 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
    36541 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
    36601 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
    36661 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
    36721 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
    36781 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
    36841 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
    36901 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
    36961 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
    37021 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
    37081 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    37141 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    37201 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    37261 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    37321 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    37381 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    37441 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    37501 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    37561 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    37621 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    37681 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    37741 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    37801 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    37861 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    37921 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    37981 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    38041 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    38101 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    38161 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    38221 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    38281 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    38341 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    38401 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    38461 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    38521 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    38581 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    38641 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    38701 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    38761 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    38821 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    38881 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    38941 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    39001 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    39061 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    39121 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    39181 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    39241 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    39301 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    39361 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    39421 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    39481 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    39541 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    39601 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    39661 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    39721 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    39781 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    39841 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    39901 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    39961 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    40021 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    40081 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    40141 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    40201 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    40261 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    40321 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    40381 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    40441 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    40501 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    40561 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    40621 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    40681 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    40741 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    40801 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    40861 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    40921 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    40981 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    41041 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    41101 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    41161 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    41221 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    41281 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    41341 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    41401 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    41461 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    41521 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    41581 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    41641 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    41701 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    41761 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    41821 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    41881 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    41941 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    42001 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    42061 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    42121 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    42181 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    42241 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    42301 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    42361 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    42421 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    42481 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    42541 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    42601 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    42661 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    42721 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    42781 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    42841 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    42901 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    42961 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    43021 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    43081 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    43141 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    43201 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    43261 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    43321 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    43381 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    43441 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    43501 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    43561 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    43621 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    43681 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    43741 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    43801 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    43861 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    43921 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    43981 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    44041 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    44101 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    44161 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    44221 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    44281 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    44341 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    44401 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    44461 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    44521 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    44581 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    44641 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    44701 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    44761 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    44821 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    44881 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    44941 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    45001 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    45061 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    45121 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    45181 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    45241 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    45301 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    45361 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    45421 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    45481 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    45541 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    45601 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    45661 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    45721 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    45781 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    45841 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    45901 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    45961 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    46021 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    46081 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    46141 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    46201 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    46261 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    46321 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    46381 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    46441 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    46501 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    46561 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    46621 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    46681 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    46741 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    46801 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    46861 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    46921 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    46981 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    47041 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    47101 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    47161 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    47221 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    47281 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    47341 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    47401 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    47461 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    47521 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    47581 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    47641 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    47701 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    47761 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    47821 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    47881 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    47941 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    48001 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    48061 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    48121 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    48181 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    48241 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    48301 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    48361 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    48421 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    48481 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    48541 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    48601 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    48661 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    48721 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    48781 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    48841 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    48901 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    48961 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    49021 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    49081 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    49141 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    49201 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    49261 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    49321 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    49381 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    49441 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    49501 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    49561 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    49621 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    49681 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    49741 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    49801 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    49861 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    49921 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    49981 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    50041 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    50101 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    50161 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    50221 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    50281 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    50341 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    50401 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    50461 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    50521 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    50581 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    50641 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    50701 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    50761 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    50821 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    50881 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    50941 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    51001 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    51061 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    51121 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    51181 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    51241 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    51301 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    51361 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    51421 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    51481 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    51541 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    51601 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    51661 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    51721 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    51781 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    51841 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    51901 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    51961 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    52021 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    52081 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    52141 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    52201 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    52261 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    52321 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    52381 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    52441 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    52501 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    52561 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    52621 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    52681 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    52741 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    52801 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    52861 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    52921 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    52981 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    53041 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    53101 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    53161 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    53221 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    53281 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    53341 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    53401 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    53461 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    53521 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    53581 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    53641 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    53701 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    53761 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    53821 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    53881 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    53941 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    54001 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    54061 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    54121 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    54181 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    54241 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    54301 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    54361 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    54421 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    54481 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    54541 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    54601 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    54661 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    54721 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    54781 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    54841 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    54901 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    54961 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    55021 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    55081 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    55141 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    55201 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    55261 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    55321 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    55381 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    55441 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    55501 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    55561 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    55621 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    55681 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    55741 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    55801 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    55861 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    55921 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    55981 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    56041 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    56101 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
    56161 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    56221 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    56281 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    56341 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    56401 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    56461 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    56521 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    56581 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    56641 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    56701 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    56761 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    56821 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    56881 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    56941 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    57001 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    57061 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    57121 ataaagtt
//
LOCUS       DACECS010000011        56574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.011, whole genome shotgun sequence.
ACCESSION   DACECS010000011 DACECS010000000
VERSION     DACECS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.011"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            336..1439
                     /locus_tag="IWO31_000711"
     CDS             336..1439
                     /locus_tag="IWO31_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0301150.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IWO31_000712"
     CDS             1723..2403
                     /locus_tag="IWO31_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0301151.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IWO31_000713"
     CDS             2493..3527
                     /locus_tag="IWO31_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0301152.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IWO31_000714"
     CDS             3541..3876
                     /locus_tag="IWO31_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301153.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IWO31_000715"
     CDS             3860..6340
                     /locus_tag="IWO31_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301154.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IWO31_000716"
     CDS             6396..7955
                     /locus_tag="IWO31_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301155.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IWO31_000717"
     CDS             7952..9316
                     /locus_tag="IWO31_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0301156.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IWO31_000718"
     CDS             9487..10557
                     /locus_tag="IWO31_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301157.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IWO31_000719"
     CDS             10572..11240
                     /locus_tag="IWO31_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301158.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IWO31_000720"
     CDS             11260..11838
                     /locus_tag="IWO31_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0301159.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IWO31_000721"
     CDS             12241..13152
                     /locus_tag="IWO31_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0301160.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IWO31_000722"
     CDS             complement(13408..14325)
                     /locus_tag="IWO31_000722"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0301161.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IWO31_000723"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IWO31_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0301162.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15569
                     /locus_tag="IWO31_000724"
                     /pseudo
     CDS             15022..15569
                     /locus_tag="IWO31_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            15684..18044
                     /locus_tag="IWO31_000725"
     CDS             15684..18044
                     /locus_tag="IWO31_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0301163.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="IWO31_000726"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="IWO31_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0301164.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="IWO31_000727"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="IWO31_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0301165.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="IWO31_000728"
     CDS             20550..21449
                     /locus_tag="IWO31_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301166.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="IWO31_000729"
     CDS             21442..22704
                     /locus_tag="IWO31_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301167.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="IWO31_000730"
     CDS             22867..24765
                     /locus_tag="IWO31_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0301168.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="IWO31_000731"
     CDS             complement(25064..25783)
                     /locus_tag="IWO31_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0301169.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26120..26452
                     /locus_tag="IWO31_000732"
     CDS             26120..26452
                     /locus_tag="IWO31_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301170.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="IWO31_000733"
     CDS             26439..28442
                     /locus_tag="IWO31_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0301171.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="IWO31_000734"
     CDS             28461..28931
                     /locus_tag="IWO31_000734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0301172.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="IWO31_000735"
     CDS             28952..29536
                     /locus_tag="IWO31_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0301173.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="IWO31_000736"
     CDS             29551..30537
                     /locus_tag="IWO31_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0301174.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="IWO31_000737"
     CDS             30527..30838
                     /locus_tag="IWO31_000737"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0301175.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="IWO31_000738"
     CDS             30860..32641
                     /locus_tag="IWO31_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0301176.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="IWO31_000739"
     CDS             32634..34010
                     /locus_tag="IWO31_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0301177.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="IWO31_000740"
     CDS             34017..34652
                     /locus_tag="IWO31_000740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0301178.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="IWO31_000741"
     CDS             34682..36037
                     /locus_tag="IWO31_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0301179.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="IWO31_000742"
     CDS             complement(36229..37623)
                     /locus_tag="IWO31_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0301180.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37715..38686
                     /locus_tag="IWO31_000743"
     CDS             37715..38686
                     /locus_tag="IWO31_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0301181.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38683..39465
                     /locus_tag="IWO31_000744"
     CDS             38683..39465
                     /locus_tag="IWO31_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0301182.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39634..40005)
                     /locus_tag="IWO31_000745"
     CDS             complement(39634..40005)
                     /locus_tag="IWO31_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0301183.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40139..40582
                     /locus_tag="IWO31_000746"
     CDS             40139..40582
                     /locus_tag="IWO31_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0301184.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="IWO31_000747"
     CDS             complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="IWO31_000747"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0301185.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41453..42820)
                     /locus_tag="IWO31_000748"
     CDS             complement(41453..42820)
                     /locus_tag="IWO31_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0301186.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42804..43634)
                     /locus_tag="IWO31_000749"
     CDS             complement(42804..43634)
                     /locus_tag="IWO31_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0301187.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43797..44234
                     /locus_tag="IWO31_000750"
     CDS             43797..44234
                     /locus_tag="IWO31_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0301188.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44310..44930
                     /locus_tag="IWO31_000751"
     CDS             44310..44930
                     /locus_tag="IWO31_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0301189.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45119..45625
                     /locus_tag="IWO31_000752"
     CDS             45119..45625
                     /locus_tag="IWO31_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0301190.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45642..46541
                     /locus_tag="IWO31_000753"
     CDS             45642..46541
                     /locus_tag="IWO31_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0301191.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46628..47512
                     /locus_tag="IWO31_000754"
     CDS             46628..47512
                     /locus_tag="IWO31_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0301192.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47704..47775)
                     /locus_tag="IWO31_000755"
     tRNA            complement(47704..47775)
                     /locus_tag="IWO31_000755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47740..47742),aa:Arg,seq:ccg)
     gene            47835..49169
                     /gene="rpoN"
                     /locus_tag="IWO31_000756"
     CDS             47835..49169
                     /gene="rpoN"
                     /locus_tag="IWO31_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0301193.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49372..50409
                     /locus_tag="IWO31_000757"
     CDS             49372..50409
                     /locus_tag="IWO31_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0301194.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50450..51451
                     /gene="gap"
                     /locus_tag="IWO31_000758"
     CDS             50450..51451
                     /gene="gap"
                     /locus_tag="IWO31_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0301195.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51561..52751
                     /locus_tag="IWO31_000759"
     CDS             51561..52751
                     /locus_tag="IWO31_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0301196.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52855..53610
                     /locus_tag="IWO31_000760"
     CDS             52855..53610
                     /locus_tag="IWO31_000760"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0301197.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53726..55024
                     /gene="eno"
                     /locus_tag="IWO31_000761"
     CDS             53726..55024
                     /gene="eno"
                     /locus_tag="IWO31_000761"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0301198.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55423..56562
                     /locus_tag="IWO31_000762"
     CDS             55423..56562
                     /locus_tag="IWO31_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0301199.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        18779 a         9857 c        11984 g        15954 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg
    15481 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    15541 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    37741 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    37801 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    37861 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    37921 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    37981 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    38041 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    38101 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    38161 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    38221 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    38281 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    38341 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    38401 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    38461 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    38521 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    38581 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    38641 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    38701 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    38761 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    38821 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    38881 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    38941 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    39001 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    39061 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    39121 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    39181 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    39241 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    39301 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    39361 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    39421 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    39481 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    39541 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    39601 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    39661 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    39721 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    39781 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    39841 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    39901 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    39961 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    40021 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    40081 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    40141 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    40201 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    40261 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    40321 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    40381 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    40441 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    40501 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    40561 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    40621 tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag
    40681 tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac
    40741 cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt
    40801 gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat
    40861 ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt
    40921 gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga
    40981 cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta
    41041 aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt
    41101 catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat
    41161 cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg
    41221 ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt
    41281 cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag
    41341 cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac
    41401 catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata ttttaattta
    41461 tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata
    41521 aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat
    41581 ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca
    41641 aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat
    41701 aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc
    41761 cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa
    41821 aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag
    41881 gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga
    41941 ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt
    42001 caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa
    42061 ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc
    42121 taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat
    42181 aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga
    42241 aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt
    42301 ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac
    42361 cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta
    42421 cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat
    42481 gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc
    42541 ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg
    42601 aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga
    42661 aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca
    42721 aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt
    42781 tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta
    42841 ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt
    42901 agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca
    42961 aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc
    43021 attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat
    43081 tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac
    43141 atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca
    43201 tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc
    43261 ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg
    43321 gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct
    43381 gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt
    43441 gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg
    43501 cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat
    43561 atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga
    43621 atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac aatattatac
    43681 tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg
    43741 acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg
    43801 aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg
    43861 aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt
    43921 acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat
    43981 tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat
    44041 ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg
    44101 ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc
    44161 gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg
    44221 attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa
    44281 ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca
    44341 aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca
    44401 tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa
    44461 ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat
    44521 taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag
    44581 taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag
    44641 tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag
    44701 atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg
    44761 aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg
    44821 atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg
    44881 atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt
    44941 cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt
    45001 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
    45061 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
    45121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
    45181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
    45241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
    45301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
    45361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
    45421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
    45481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
    45541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
    45601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
    45661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
    45721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
    45781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
    45841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
    45901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
    45961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
    46021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
    46081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
    46141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
    46201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
    46261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
    46321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
    46381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
    46441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
    46501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
    46561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
    46621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
    46681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
    46741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
    46801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
    46861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
    46921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
    46981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
    47041 aatcgtgaag cagacagctg aagcagggca cgagattggc aattacacat acgatcatca
    47101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
    47161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
    47221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
    47281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
    47341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
    47401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
    47461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
    47521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
    47581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
    47641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
    47701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
    47761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
    47821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
    47881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
    47941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
    48001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
    48061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
    48121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
    48181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
    48241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
    48301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
    48361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
    48421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
    48481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
    48541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
    48601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
    48661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
    48721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
    48781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
    48841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
    48901 atttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
    48961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
    49021 aagaagacaa aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg
    49081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
    49141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
    49201 ttttctagtc tttttagttg aaaaacgaga gcgaaactgt tagacttaca gtcgtgaggt
    49261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
    49321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
    49381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
    49441 aaaattttac gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt
    49501 atggggatca cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg
    49561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
    49621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
    49681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
    49741 gctgatgcat tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag
    49801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
    49861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
    49921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
    49981 cgcgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
    50041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
    50101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
    50161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
    50221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
    50281 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
    50341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
    50401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
    50461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
    50521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
    50581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
    50641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
    50701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
    50761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
    50821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
    50881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
    50941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
    51001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
    51061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt
    51121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
    51181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
    51241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
    51301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
    51361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
    51421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
    51481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
    51541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
    51601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
    51661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
    51721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
    51781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
    51841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
    51901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
    51961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
    52021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
    52081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
    52141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
    52201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
    52261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
    52321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
    52381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
    52441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
    52501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
    52561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
    52621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
    52681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
    52741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgaacaa aaggtgaagg
    52801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
    52861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
    52921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
    52981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
    53041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
    53101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
    53161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
    53221 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
    53281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaggt gtcaaacatg
    53341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
    53401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
    53461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
    53521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
    53581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
    53641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
    53701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
    53761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
    53821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
    53881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
    53941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
    54001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
    54061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
    54121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
    54181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
    54241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
    54301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
    54361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
    54421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
    54481 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
    54541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
    54601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
    54661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
    54721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
    54781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
    54841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
    54901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
    54961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    55021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    55081 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc
    55141 aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa
    55201 aggaaagcta acaataacta gcatcatata agagctttta aggatagaac agtagaatag
    55261 gaatctatgg gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt
    55321 cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca
    55381 cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa
    55441 atcgcgattt tacaagagat tatccgaatt aaatcagtga atggaaacga aggtgaagta
    55501 gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat tgtttcttac
    55561 agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta
    55621 ggcctatctg gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca
    55681 ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag
    55741 agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg aaaaccattt
    55801 aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact aggtggggaa
    55861 cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg agagccaacc
    55921 aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac ctcgcacgga
    55981 aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca cctaaatgaa
    56041 ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc
    56101 ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt caacagcatc
    56161 ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc aaatgataaa
    56221 atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga ctatcatttg
    56281 gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc tcctttgatt
    56341 cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc agcaacaacg
    56401 gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt tgggcctggt
    56461 gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta tttggatatg
    56521 atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt taat
//
LOCUS       DACECS010000012        56523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.012, whole genome shotgun sequence.
ACCESSION   DACECS010000012 DACECS010000000
VERSION     DACECS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.012"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO31_000763"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO31_000763"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0301200.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWO31_000764"
     CDS             complement(937..1518)
                     /locus_tag="IWO31_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0301201.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO31_000765"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO31_000765"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0301202.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO31_000766"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO31_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0301203.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO31_000767"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO31_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0301204.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWO31_000768"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWO31_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0301205.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWO31_000769"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWO31_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0301206.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO31_000770"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO31_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0301207.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWO31_000771"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWO31_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0301208.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWO31_000772"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWO31_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0301209.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWO31_000773"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWO31_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0301210.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWO31_000774"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWO31_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0301211.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO31_000775"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO31_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0301212.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWO31_000776"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWO31_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0301213.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO31_000777"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO31_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0301214.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWO31_000778"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWO31_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0301215.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO31_000779"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO31_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0301216.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO31_000780"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO31_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0301217.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWO31_000781"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWO31_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0301218.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWO31_000782"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWO31_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0301219.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWO31_000783"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWO31_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0301220.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWO31_000784"
     CDS             13972..14157
                     /locus_tag="IWO31_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0301221.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO31_000785"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO31_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0301222.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWO31_000786"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWO31_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0301223.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWO31_000787"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWO31_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0301224.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO31_000788"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO31_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0301225.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO31_000789"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO31_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0301226.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWO31_000790"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWO31_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0301227.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWO31_000791"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWO31_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0301228.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWO31_000792"
     CDS             18232..18879
                     /locus_tag="IWO31_000792"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0301229.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWO31_000793"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWO31_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0301230.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO31_000794"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO31_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0301231.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO31_000795"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO31_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0301232.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO31_000796"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO31_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0301233.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWO31_000797"
     CDS             20317..21255
                     /locus_tag="IWO31_000797"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0301234.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO31_000798"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO31_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0301235.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWO31_000799"
     CDS             21935..22774
                     /locus_tag="IWO31_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0301236.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWO31_000800"
     CDS             22750..23619
                     /locus_tag="IWO31_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0301237.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWO31_000801"
     CDS             23609..24406
                     /locus_tag="IWO31_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0301238.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IWO31_000802"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IWO31_000802"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0301239.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IWO31_000803"
     CDS             complement(25688..28357)
                     /locus_tag="IWO31_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0301240.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IWO31_000804"
     CDS             complement(28595..28783)
                     /locus_tag="IWO31_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0301241.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IWO31_000805"
     CDS             29053..29415
                     /locus_tag="IWO31_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0301242.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IWO31_000806"
     CDS             29467..31149
                     /locus_tag="IWO31_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0301243.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IWO31_000807"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IWO31_000807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0301244.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IWO31_000808"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IWO31_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0301245.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IWO31_000809"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IWO31_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0301246.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IWO31_000810"
     CDS             34984..35988
                     /locus_tag="IWO31_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301247.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IWO31_000811"
     CDS             35989..36933
                     /locus_tag="IWO31_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301248.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IWO31_000812"
     CDS             36933..37895
                     /locus_tag="IWO31_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301249.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IWO31_000813"
     CDS             37922..38836
                     /locus_tag="IWO31_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301250.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IWO31_000814"
     CDS             38862..40643
                     /locus_tag="IWO31_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0301251.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IWO31_000815"
     CDS             40991..41677
                     /locus_tag="IWO31_000815"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0301252.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IWO31_000816"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IWO31_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0301253.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IWO31_000817"
     CDS             45287..46096
                     /locus_tag="IWO31_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0301254.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IWO31_000818"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IWO31_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0301255.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IWO31_000819"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IWO31_000819"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0301256.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IWO31_000820"
     CDS             complement(49648..50100)
                     /locus_tag="IWO31_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0301257.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IWO31_000821"
     CDS             50250..51200
                     /locus_tag="IWO31_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0301258.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IWO31_000822"
     CDS             51193..52152
                     /locus_tag="IWO31_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0301259.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IWO31_000823"
     CDS             52149..52904
                     /locus_tag="IWO31_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301260.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IWO31_000824"
     CDS             52927..53880
                     /locus_tag="IWO31_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0301261.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54403..54699)
                     /locus_tag="IWO31_000825"
     CDS             complement(54403..54699)
                     /locus_tag="IWO31_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0301262.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55054..55410
                     /locus_tag="IWO31_000826"
     CDS             55054..55410
                     /locus_tag="IWO31_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0301263.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55420..56319
                     /locus_tag="IWO31_000827"
     CDS             55420..56319
                     /locus_tag="IWO31_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0301264.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        18543 a        10222 c        12116 g        15642 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcgg cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    54301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg
    54361 accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc
    54421 aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca
    54481 ccgttttaat aattgataga aataactctc ttctgctttg attttatact cgcttaatat
    54541 caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt
    54601 tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt
    54661 ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc
    54721 ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat
    54781 tctttttcca atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga
    54841 aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact
    54901 gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga
    54961 cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat
    55021 aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca
    55081 caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac
    55141 ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg
    55201 atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca
    55261 ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca
    55321 cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa
    55381 ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc
    55441 agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa
    55501 gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc taataaagta
    55561 gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat
    55621 ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac
    55681 ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg
    55741 aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg
    55801 aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat
    55861 gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag
    55921 gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct
    55981 tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt
    56041 gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat
    56101 aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag
    56161 ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta
    56221 cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa
    56281 gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa
    56341 aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat
    56401 atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac
    56461 tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat
    56521 ata
//
LOCUS       DACECS010000013        55907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.013, whole genome shotgun sequence.
ACCESSION   DACECS010000013 DACECS010000000
VERSION     DACECS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.013"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            210..1424
                     /locus_tag="IWO31_000828"
     CDS             210..1424
                     /locus_tag="IWO31_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0301265.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1455..2882
                     /locus_tag="IWO31_000829"
     CDS             1455..2882
                     /locus_tag="IWO31_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0301266.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2905..3831
                     /gene="glsA"
                     /locus_tag="IWO31_000830"
     CDS             2905..3831
                     /gene="glsA"
                     /locus_tag="IWO31_000830"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0301267.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4039..5499
                     /locus_tag="IWO31_000831"
     CDS             4039..5499
                     /locus_tag="IWO31_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301268.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5720..7483
                     /locus_tag="IWO31_000832"
     CDS             5720..7483
                     /locus_tag="IWO31_000832"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0301269.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7567..8025
                     /locus_tag="IWO31_000833"
     CDS             7567..8025
                     /locus_tag="IWO31_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0301270.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8134..8817
                     /locus_tag="IWO31_000834"
     CDS             8134..8817
                     /locus_tag="IWO31_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0301271.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8964..9578
                     /locus_tag="IWO31_000835"
     CDS             8964..9578
                     /locus_tag="IWO31_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0301272.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9594..9800
                     /locus_tag="IWO31_000836"
     CDS             9594..9800
                     /locus_tag="IWO31_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301273.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            10339..10896
                     /locus_tag="IWO31_000837"
                     /pseudo
     CDS             10339..10896
                     /locus_tag="IWO31_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10954..12993
                     /locus_tag="IWO31_000838"
     CDS             10954..12993
                     /locus_tag="IWO31_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0301274.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVIGETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13102..14253)
                     /locus_tag="IWO31_000839"
     CDS             complement(13102..14253)
                     /locus_tag="IWO31_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0301275.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14407..14937)
                     /locus_tag="IWO31_000840"
     CDS             complement(14407..14937)
                     /locus_tag="IWO31_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301276.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15036..15386)
                     /locus_tag="IWO31_000841"
     CDS             complement(15036..15386)
                     /locus_tag="IWO31_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0301277.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15543..17072)
                     /gene="ahpF"
                     /locus_tag="IWO31_000842"
     CDS             complement(15543..17072)
                     /gene="ahpF"
                     /locus_tag="IWO31_000842"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0301278.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17082..17645)
                     /gene="ahpC"
                     /locus_tag="IWO31_000843"
     CDS             complement(17082..17645)
                     /gene="ahpC"
                     /locus_tag="IWO31_000843"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0301279.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17789..18451)
                     /locus_tag="IWO31_000844"
     CDS             complement(17789..18451)
                     /locus_tag="IWO31_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0301280.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18635..20281
                     /locus_tag="IWO31_000845"
     CDS             18635..20281
                     /locus_tag="IWO31_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0301281.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20272..21354
                     /gene="uxuA"
                     /locus_tag="IWO31_000846"
     CDS             20272..21354
                     /gene="uxuA"
                     /locus_tag="IWO31_000846"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0301282.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21462..23015)
                     /locus_tag="IWO31_000847"
                     /pseudo
     CDS             complement(21462..23015)
                     /locus_tag="IWO31_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            23163..24304
                     /locus_tag="IWO31_000848"
                     /pseudo
     CDS             23163..24304
                     /locus_tag="IWO31_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24430..25509)
                     /locus_tag="IWO31_000849"
     CDS             complement(24430..25509)
                     /locus_tag="IWO31_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0301283.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25618..26058)
                     /locus_tag="IWO31_000850"
     CDS             complement(25618..26058)
                     /locus_tag="IWO31_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0301284.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26115..26525)
                     /locus_tag="IWO31_000851"
     CDS             complement(26115..26525)
                     /locus_tag="IWO31_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0301285.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26706..27140)
                     /locus_tag="IWO31_000852"
     CDS             complement(26706..27140)
                     /locus_tag="IWO31_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0301286.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27240..28088)
                     /locus_tag="IWO31_000853"
     CDS             complement(27240..28088)
                     /locus_tag="IWO31_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0301287.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28477..30243
                     /locus_tag="IWO31_000854"
     CDS             28477..30243
                     /locus_tag="IWO31_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0301288.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30345..31604
                     /locus_tag="IWO31_000855"
     CDS             30345..31604
                     /locus_tag="IWO31_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0301289.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31751..33052
                     /locus_tag="IWO31_000856"
     CDS             31751..33052
                     /locus_tag="IWO31_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0301290.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33055..33906
                     /locus_tag="IWO31_000857"
     CDS             33055..33906
                     /locus_tag="IWO31_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0301291.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33919..34734
                     /locus_tag="IWO31_000858"
     CDS             33919..34734
                     /locus_tag="IWO31_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0301292.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34885..36051
                     /locus_tag="IWO31_000859"
     CDS             34885..36051
                     /locus_tag="IWO31_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0301293.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36281..36928
                     /locus_tag="IWO31_000860"
     CDS             36281..36928
                     /locus_tag="IWO31_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0301294.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37012..37956
                     /locus_tag="IWO31_000861"
     CDS             37012..37956
                     /locus_tag="IWO31_000861"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0301295.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38091..39542
                     /locus_tag="IWO31_000862"
     CDS             38091..39542
                     /locus_tag="IWO31_000862"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0301296.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39563..40399
                     /locus_tag="IWO31_000863"
     CDS             39563..40399
                     /locus_tag="IWO31_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0301297.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40399..41058
                     /locus_tag="IWO31_000864"
     CDS             40399..41058
                     /locus_tag="IWO31_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0301298.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            41184..41873
                     /locus_tag="IWO31_000865"
     CDS             41184..41873
                     /locus_tag="IWO31_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301299.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41879..42835
                     /locus_tag="IWO31_000866"
     CDS             41879..42835
                     /locus_tag="IWO31_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0301300.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42879..43391
                     /locus_tag="IWO31_000867"
     CDS             42879..43391
                     /locus_tag="IWO31_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301301.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43647..44195)
                     /locus_tag="IWO31_000868"
     CDS             complement(43647..44195)
                     /locus_tag="IWO31_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0301302.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44284..45465)
                     /locus_tag="IWO31_000869"
     CDS             complement(44284..45465)
                     /locus_tag="IWO31_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0301303.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45701..47209)
                     /locus_tag="IWO31_000870"
     CDS             complement(45701..47209)
                     /locus_tag="IWO31_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0301304.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47368..47559
                     /locus_tag="IWO31_000871"
     CDS             47368..47559
                     /locus_tag="IWO31_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301305.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47762..48679
                     /locus_tag="IWO31_000872"
     CDS             47762..48679
                     /locus_tag="IWO31_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0301306.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48672..49745
                     /locus_tag="IWO31_000873"
     CDS             48672..49745
                     /locus_tag="IWO31_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301307.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49893..50411
                     /locus_tag="IWO31_000874"
     CDS             49893..50411
                     /locus_tag="IWO31_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301308.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50392..50784
                     /locus_tag="IWO31_000875"
     CDS             50392..50784
                     /locus_tag="IWO31_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0301309.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50860..51795
                     /locus_tag="IWO31_000876"
     CDS             50860..51795
                     /locus_tag="IWO31_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0301310.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51928..52707
                     /locus_tag="IWO31_000877"
     CDS             51928..52707
                     /locus_tag="IWO31_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0301311.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52700..53017
                     /locus_tag="IWO31_000878"
     CDS             52700..53017
                     /locus_tag="IWO31_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0301312.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53261..54871
                     /locus_tag="IWO31_000879"
     CDS             53261..54871
                     /locus_tag="IWO31_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0301313.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55028..55876
                     /locus_tag="IWO31_000880"
     CDS             55028..55876
                     /locus_tag="IWO31_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0301314.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        18535 a         9920 c        10900 g        16552 t
ORIGIN      
        1 aaaaaaagac gaaaataaac ggttttctat aaaaagaaga ccgtttattt tttgtcttta
       61 tttgtgtgaa aaacgaaaaa gagcacaaca catcaagctt ttattaattg tctgtctttt
      121 ataaatgcaa gtattaattc aaaaaaacag aagaaaaaaa caactacacc ttcttttata
      181 ctcgtaatcg tagaaaggag gcgaaaaaaa tgaatacatt acctatcaat acaggagata
      241 cagcattcat gattttatgt acagcaatgg tttgcctgat gactcctggt ctagcttttt
      301 tttacggcgg attagcacgg aagaataata tactaacgat catgtctcaa tcttttctct
      361 ccgttgggat aacaacgatt atgtgggtat ttggtggatt cggattggcc tttggacggg
      421 atatcggcgg tgttattgga aacccggcag atttcttttt gatgagacat gttacaagct
      481 tccccaacat gtttcatggt gcaacgattc catttttaat gttctttctt taccaactga
      541 tgtttatcgt gattaccgtt cctttgatga caggagcttt tgcagggaga ttgaatttaa
      601 aaggctatat tatacttgtt attttttgga atctgctgat ttatttccct gtttgccact
      661 ggatatgggg tggcggattt ttggatcaga tgggatttag agatttcgct ggtggagcag
      721 tcatccacac gacagctgga ttcggctctt tagcatgtat cctcacactt ggtcaaagaa
      781 agattccttt aggagaaaga agtcagcata gcagtcttat ggcagctgct atcggtacag
      841 gactgctttg gtttggctgg ttcggtttta attccggtgg tgcacttcga gcagatgaac
      901 aagcagtcaa tgcatttggt tcaacattca tcgctcttgc atttgcgatg attacttggc
      961 tgatcattgc aaaagtaaaa ggaaatggct ttgattttgt cgacgtacta acaggatccg
     1021 ttgctggatt ggcagccatt acgccttgtg ccggttatgt agaagcaaaa agtgccatgc
     1081 tgattgggat cattgcgggt atcgtctgtc atgctgctgt tgattttcgt aaaaagaaac
     1141 aatgggatga tgcacttgat gtgtggggtg ttcatggcat gggcggattc acaggtacga
     1201 ttctgatagg gatctttgca tctaacagcc atttactaac gaatccaagc agctggtact
     1261 tcttagggat tcaagtgatt ggtgtcatca ttactgccgt gtatgcctat gtgctgacaa
     1321 cagtgatttt aaaatcggcg atgcacttta caacgatcac tactacaaaa gaagaacaag
     1381 aagaaggatt ggactttttc ttctttggca acaaactcaa ttgaaacaaa ttcaaataga
     1441 aaagaggaag attcatgcaa caaagcaaaa cgaaaattcc tgcaaaaaaa ttaacgttct
     1501 ttggcttttt ggctatgacc atctcaatgg tcgtttcatt atatgaatat cccacattcg
     1561 cgacctcagg cttcagctta gtatttttct tgttattagg cggtttgcta tggtttatcc
     1621 ctgtggcatt gtgcgcagca gagatggcaa cggtgaaagg atgggaaaaa ggcggtgtct
     1681 atacttgggt cagtcgcaca ttgggaaaac gattcggttt tgccgcaatc tttttccaat
     1741 ggtttgagat aacagttggc tacttgacca tgctgtattt cttaactggt gcattatcct
     1801 atgcaacggg tattggtgct atacaaaaca ataaatatct gaaattggca atcctattga
     1861 ttattttctg ggctatttta ctctcacagt taaaaggaac caaatacact tcattgattg
     1921 ctcggatcgg ttttgtagct ggtatcttgt tgccagcatt agtcttgttt ggcttaggga
     1981 tccattatgt cgcttcaggc gctccactac aaacgatact gtctatgaaa acattgattc
     2041 ctgatttttc gaaattgcct acgctggttg tttttgtttc ctttatttta gcatatatgg
     2101 gcgtagaaac atctgccagt catgcaaatg aaatggaaaa ccctaaaaag gattatccat
     2161 tagcaatgtt tgtattggtt attctggcaa tcattttaga tacttttggc ggattgaccg
     2221 tcgctactac agtacctcaa catggtttgt ctcttaatac aggggtgatt caatcgttag
     2281 aattcctatt aagacacatt gatccaaatc ttgcatggat tgcgaaaatt cttgcgattc
     2341 ttgtttgttt aggtgtcatc ggagaaattg cttcttgggt aacctctcct tcaaaagcac
     2401 ttcacgtagc agcagaagaa ggattacttc ctgaatattt tgcaaaagaa aacacacacg
     2461 gtgttcctgc tcatttgatg atcgcaaatg ggatcgttgc aacaatctgg gcagccgtct
     2521 ttacattaag cggcgggggg aataatcttt catttcttgc agcgatgtca ttgactgttg
     2581 taatctacct gatgttgtac tttatgtttt tcgcgggcta tttgaagctg gtttttaaag
     2641 atgacagtct tgaaagagcc tatcaggttc caggcggaaa agttgtaaaa gtaatcgttg
     2701 cttgtttagg tcttttgact tcactttttg ctttttgtat ctcatttgtt ccacctgcta
     2761 gtatcgcagc tggacagcat gggatttatg aaacgattct gattgtttct tatcttatcg
     2821 tcttagttat tccatttacg atctacgcat gccgtcataa attccagcat gcagaaaaat
     2881 aaaaaatgaa agctggttat tcaaatggaa gtaaaagaat tagaacaatt gattggaaaa
     2941 aatttccact attatcaaac aggacaagtg gcggattaca ttccagcatt agctgaagtg
     3001 gatcccaaac aattaggtat ggcgatttat gatttggata caaaacaact gattgaggga
     3061 ggagacgccg atgtgcgctt tgccatcgaa agcatgtcaa aagttcccgt attgttatta
     3121 gcaatcaaag acaacggaat agaaaaagtc tttcaaacaa tcaacacgga accaaccggt
     3181 tttgccttca attctatcat gaatatggaa atcaatcatc gcaaacatcc aatgaatcct
     3241 tttgttaatg caggagcgat tgcaacaacc tcgttgattg ccggtaaaga tgcagatgaa
     3301 aaatttgagc gaatcttgtc atttatgaaa gagatctgtg atgatcctaa tatcacattg
     3361 aatgaagaaa tctatcattc tgaatcaaga acaggtgata tcaatcgctc tcttgcttac
     3421 tatatgaaag gtaatcaaat gatcgaagga gacgtaccgg agatactcga tgcttatttc
     3481 agacaatgct ccctcaatgt caccgctaca ggcatagcaa aattagctgc tgtgctggca
     3541 aataaaggaa ttgctccttg gaatggcaaa caaatcatta ctgaagaaag tgctacaatc
     3601 gtcaaatcaa ttatgacaac tgctggtttg tatgatgaat caggagagtt ttccgtccat
     3661 gttggtgtac ctgcaaaaag cggtgtaggc ggcgggttga tggcagctgt gccaaatcgt
     3721 tacggcatag gtgtcttcag cccggcacta gatccatttg gtaacagcgc agctggtatc
     3781 caattgttga aagatgtagt caaagaacta gacgctgaca tctttgaata accattcttg
     3841 aaagaggctg gggtaaaagt ccaaacggct caaagctacc tacaatgagg ctgaaattgt
     3901 gagcttgttt gacttttgtc ccagtttctt tttttattgg tttttgaaag ggcttttcta
     3961 aataaaaagg aaacaaaaat acgatttaca gatatgatta aataaaatca taagaaaaac
     4021 tggaggcgtg tatcaggaat gctggaaaat tacatcgagc gaaatatatt tagaaaagtg
     4081 tatctgtgtg aacaattatt tgaattccaa gaaatagaca tcgaacaaac agctatttct
     4141 ctaagggtga ctacaccaac gatccttcac gatctagaaa gtctagccga gtgtctagaa
     4201 tattgtataa aagaacaagt aagagagaag cataagtata aactcgtatt caaacatggt
     4261 attgctcttt ctgagctcac acagtttctg tacggtcaat cttatttttt aaagttttta
     4321 agttattatt ttaatggtat attcacgagt acagaactgg ctgatcttga atttatttct
     4381 ttgagcaaag tatatacaat caaaaaaatc gtattggatt tttttaaaga aaatgattat
     4441 ttgaaaaata aagaaatcat tatcccagaa tttgactcgc ggaacatctt gctagcttta
     4501 gtacgttatg tcaattggga aggctatgaa aataaaaatc cgtatgtaga agaagcttgt
     4561 aacgagttga ttgattatgt agagactcat ttttttaaaa gaagatattc ggaagatgaa
     4621 aagtccttta tcatacgagg aatagagatc gcaatcggaa gaaaggcatt tccccttgga
     4681 ctgaaagaag aagataagaa aagcgcagaa acgaagccgt tatttcaatt agtctgtcaa
     4741 ggattggaag agcaaaaagg aattctagac ttacaagaag aagatgtcta ttatattttt
     4801 tctttgttca acagtagaaa ttataccaat gagaacatgg agctgttaag aaaagatttc
     4861 gaagttgttt ataaaaattt tgttctcaat gacctatcac tgaatgaatt gattttacag
     4921 attattagta tcgctgaaat cgacgatgca gaaaatttta ttttcaagca atccttttta
     4981 caatttgtcc gtacgttgtg ggcagatgga caagtatttt taccggaaag gatctatctt
     5041 ctatcagaaa aagaacaact tctgtacgga aaaattgtag aaatattgga agagtggaaa
     5101 aagaaaaatc aaatccaact aagatggaat gaaaatttca ttcgaaaatt cgtcaaaagt
     5161 ctttctttga tcaacagtga acaagcgggg agacaggaaa tagaaatttt tgttgtttca
     5221 gaaagttcag tcaaacaatt tttttatcga acccaatttc atttgtatgc ggaagaaggc
     5281 atcgctgaaa taaatcctat tttatttcgt tcattagaag aattgcctga tgaatgtcta
     5341 tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct atcaaaatgg attagaaact
     5401 gaaaagacaa agatctatcc aatctcatta aaaggtgcaa acatccattt gtatcatatt
     5461 gctcaggaac ttcacctatt aaaaaaacat atttcttgat gagaccttaa ttaggagacc
     5521 ttctacaaag ctttttaatt gtctgtagaa ggtttttatt tgagtcctga ttcgtgcgaa
     5581 gatctactta tataacaagc tttctctgaa atttttttaa aatgctattg acttagagtc
     5641 aactctaagt agtagaatag ataaagtgta acaaatctat tttcattaaa agaagatagt
     5701 acggaggaga gaatgtatta tgagtacagt attacatgta ttggatgcaa tcaatagccc
     5761 cgcagatgtc aaaaaattat ctgttgagga aatgaaacag ttggcagaag agatgcgcca
     5821 aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt ggtccgaatc taggtgtagt
     5881 agaattaacg attgcgtttc attatgtatt tgattcacca aaggataaaa tcgtttggga
     5941 tgtttcccat cagtcttatg gtcataagct tttaactggt cgaaaaaatg gttttgaaaa
     6001 aggacattac cgtgaagtga gcggatattc caaccaacat gaaagtaaac atgactattt
     6061 tacagtgggg cacacatcta catctatcag cttagcaaca ggtttagcta aagcgagaga
     6121 tctgaagaaa gaatcaggaa atgtggtagc atttatcggt gacggctccc taagtggtgg
     6181 cttagctttt gaaggattga acaatggtgg tgttttacaa tccaatctaa tcgtattagt
     6241 aaacgataat gagatgtcta ttgatgagaa tcatggcagt ttatataaaa acttagctga
     6301 gcttcgtcaa accaacggta aaagcagcaa taacctattt aaagcaatgg gatttgatta
     6361 tcgttacgtg gataatggaa acgatctgga aacggtgatc cgtttatttg aagaagtaaa
     6421 agatattgat catccgattg tacttcacgt ccatacggaa aaaggtcgcg gttatcagcc
     6481 ggcaatcgat aataaaatga aatatcattg gcatgtacca tttgatctgt ccaccgggga
     6541 ggcaaagcaa gtaacttctg gagaaacatt taatatgatc atcatggatt atttggataa
     6601 gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat gcagcgatac caggcgcctt
     6661 tggactgaaa acattccaag aaaaacatcc agatcattat tttgacgtgg ggatcgctga
     6721 acagcactct atcactttcg cttctgcttt ggcttctcag ggagtacgtc cagtcgtctt
     6781 ccaatcaagt actttcttgc aacgggcata tgatcaatta tcacatgatt tagcgatcaa
     6841 cgagaatcca gtgattattt tagtcggtgg cggaacgatc tcagctagtg accgaacaca
     6901 ccaaggaaca tttgacattc cttacttatc aagtatcccg aacatcaaat atcttgctcc
     6961 agcaacaaaa gaggatatga tctctatgct agattgggcg ttcgaacaaa cagcgcagcc
     7021 tgtagcaatc cgaattccta cacatggtgt agaacatggt acagctatcg caaatgatta
     7081 tagtattcca gcctatcaaa cagtaaataa tggaggaaaa gtagcaattt tagctttagg
     7141 cggttttctt gaattaggaa aagaaacagt aaaagctttt agaaaaaaag gaatcgaagc
     7201 aacgttgatc aatccaggaa cgatcacaga aaatgacgta gaaacattag ataaattaaa
     7261 agaaacacac gaattagtca tcacattaga agatggtagt ttgtcaggag gatttggcga
     7321 aaatatcagt cgttattatg gtgattcttc aatgaacgta ttaaattttg gtgcagagaa
     7381 agaattcacg gatagcgttc ctgtagggca gctttatgaa cgttatcatt taacaccaaa
     7441 acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa taattcatta tgctcaccat
     7501 ccagataatt gtttatacta gtatctggat ggttttttca ttataaaaag caaaggggtg
     7561 gatgttatgt atcagcaagt agaagaatat gtcaaaaagt tccaagaaaa atatcctagt
     7621 tatcaatcag aaaaattagg tttcttttca tttggggata cagaaaaaat ggcagacgag
     7681 ttgagcgaac tagttgtaaa aaagataaag actggtacat caagtggtta cgatctgtat
     7741 aaacaagaga atgagccttt accaaaagtt ggtcaattag acgtggtcct gaatggaaaa
     7801 aatgaaccag tttgtatcat caaaaatata aatgtgacca tcttgccttt tagtgaagtc
     7861 acgaaagagc atgcttggaa agaaggagaa ggggatcgga cattagctta ttggcggaaa
     7921 gtccatgctg agttttttaa acatgcttat aaagaagcgt tgaacataga atttacagaa
     7981 gagaagctgg tcgtatacga agaatttgaa gtaattttct tttagtaaga aaggtctgct
     8041 gaaagaaata gctcattatt gtctcctgaa tagaaaaggg ttacattttt atatgaagct
     8101 gtatttttat ataacattac tggaggagat attgtggata cactggaaca aatcaaaggc
     8161 ggagtcgtcg tttcttgtca agctttggaa aacgagccgc tacacagttc ttttattatg
     8221 gggagaatgg ctttggctgc aaaagaaggc ggagcagttg gtattcgagc aaatacgaca
     8281 gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg taatagggat tatcaaaaga
     8341 gattacgatg ggtcagaagt gtttattact gcgacagaaa aagaagtgga agaacttttg
     8401 gagacgcaag cagaaatcat tgcactggat gcaacttctc gtaaacgtcc gacagaaaaa
     8461 acaactgctg aacttgtcca aatgattcac gaacacggtc ggttagctat ggcagatatt
     8521 tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt ttgatctttt gtctacgact
     8581 ttagcaggct atacagaata ttcacgaaag acgcagacac ctgattttga tttattgaat
     8641 caaatcatag aacaagtcga tatcccagta attatggaag gacatactga ctctcctgaa
     8701 caagtagaga aagcatatga attaggtgct ttttcagtcg tagttggatc tattatcacc
     8761 cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa gtaaagcaaa taactaaaaa
     8821 acacgcacgg cacagattct tgacaaaaaa gaaaaattga tagatagttt aaagcagatt
     8881 ctaagaagat gaaaaaagca ttacttttca ttttgtcttc aagatctgct ttttaacgaa
     8941 ttttatagaa agaaggcgaa accatgtttg aacggagtcc ttcagaattg acagaacgtg
     9001 aaaattacaa gttgctgatt ggttcagtca tccctagacc agtagcagta gtaacgacta
     9061 aatcagcaaa agatatagtg aatatcgcac ccttcagtta cttcaatatc gtcagttcga
     9121 atcgacccat cttatccttg gcgatccaga gaaaacaaga tgcagtaaaa gacacagcaa
     9181 agaatattct tgagacaaga gaagctgtta tccatatcct tgatgaggac aacgtagaag
     9241 aagtaaataa aacagcagcc aatctgccta atgaggaaag cgaactgtac ctgactgact
     9301 tgactttaac tgattcagta atcatttcag taccagcatt gacagaagca aaagttcgtt
     9361 ttgaaacatc cttatatgaa catgtcgaga tacggaataa aaataaagta acagctgatt
     9421 tatttttgct agaagtacag aaatatcatt taaatgaaac agtctatgat gataagacag
     9481 gaaggatcga tccaagaaaa ttgaatgctg taagccgttt ggcaggaaat gattatgcag
     9541 caataggaga aattttcacg attgcacgtc ccgaataaaa aggagcacta gttatgttaa
     9601 aaataagcta taaaccatcg acagattcaa aagaaatgaa aaaagaatat gaaacagtga
     9661 atgattttct gcaagggcag tacttagaag ttccgccatt gcaagaccat tttgtcgtta
     9721 ctactgttac tttagatggt aaggaaatag aaatgccaga tcaaacgatt agcggattgt
     9781 ttaattactt taacaaataa atatttattc cgaaaagtga aaaaacatat aaaaaaacac
     9841 ataaaactaa aaaattatca aagaatcctg gtgcaactga aagaatttgc aacgggattc
     9901 tttcaactta tttgttattt ttttctaaaa aatattaaag attgttaaat ttcactattt
     9961 catttcaaat tccattgcct aatcattagg aaaagtctat catgaactcg taaatagaat
    10021 atagtaataa ccttgttagg tgaggctcct atacaaacat aggctgttgc ccagaaacgt
    10081 cgaaagtcgc caatgggtaa aacagaaact gttggattaa ggctttttcc aatagctaag
    10141 aatgttttct ttacgttgta tagtgctaaa actcggacga tgagatgatg ctaggtcaga
    10201 attcttgttc gaaaaggttt ctgacctttt ctttatattt ttgtgactga cttatttgat
    10261 gagacaaact cgctcctcac ctgacaagtg gattgctgtg tgaggaggag atgcggtgaa
    10321 aaaagatcga ttggtggagg tcgctcggat tcacttctgg ttgcacaata caaccagaaa
    10381 acacaacaag caaaactgat ctctatcatg agagattcat atgtcgatat tccaggttac
    10441 ggaatgaata agataaatgc agcttactct tatgggggag ttgatttatt aaatcaaaca
    10501 ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa gtatcacatt tcaagatttt
    10561 atcgattgcg ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta
    10621 gatttagatg gcgtatatat aaacaaagga gagcaagtga tggatggtaa tactttactc
    10681 cagtatgctc gatttcggga agacaaagaa ggagacttcg ggaggatcag aaggcagcag
    10741 caagtgatca aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc
    10801 aaagcggttg ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg
    10861 gattgtggta tggatttcct caaagacata tgttagaata aacaggtata aatagtgaaa
    10921 taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa
    10981 aatcgaacag gcgcttatat tgccggcgca gtgatcttaa tagcagctgc gggtggcggt
    11041 tatttttact accagcacta tcaagaaacc caagcagtag aagctggaga aaagacagtt
    11101 gagcaatttg tccaagcttt gaacaaggga gattataaca aagctgcaga aatgacatca
    11161 aaaaaggcgg caaataaaag tgcattatct gaaaaagaga tcttagataa ataccagaat
    11221 atatacggtg ctgctgatgt caaaggactt cagatatcga atctaaaagt agataaaaaa
    11281 gacgattcta cttacagttt ttcatataaa gcaaagatga atacctcatt aggtgaattg
    11341 aaagatcttt cttataaagg aacattagac agaaatgatg gtcaaaccac gatcaactgg
    11401 caacccaact tggtttttcc agaaatggaa ggaaatgaca aagtaagcct gaccacacaa
    11461 gaagcagcaa gagggaatat tatagatcga aatggtgaac cattagcaac aacaggcaaa
    11521 ctaaaacaat taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa aacagccaat
    11581 atcaaagcga ttgcttcctc cttcgactta acagaagatg ctatcaatca ggcgatttca
    11641 caaagctggg tacaacccga ttactttgtc ccattgaaaa tcattgatgg agcaacgcca
    11701 gaacttccag ctggagctac cattcaagaa gtagacggca gatattatcc tttgggtgaa
    11761 gcagctgctc agctgattgg ttacgtggga gatatcacag cagaagatat tgataagaat
    11821 ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc ttttgataag
    11881 gatcttcgtg ggacaacagg tgggaaatta agcattacag atgcagacgg tgtagagaaa
    11941 aaagttctga tcgaacatga agttcaaaat ggcaaagata tcaaattgac aatcgatgcg
    12001 aaggcacaaa aaatagcttt tgacagtcta ggaggaaaag ctgcatcaac tgttgcgaca
    12061 acgccaaaaa ccggtgatct tcttgcgctt gctagctctc caagctatga tccaaacaaa
    12121 atgacaaacg ggatctcaca agaagattac aaagcttatg aagaaaatcc tgaacatcca
    12181 ttcatcagcc gatttgcgac aggttatgct cctggatcta cgtttaaaat gattacagca
    12241 gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag tgttgacgat caacgggctt
    12301 aaatggcaaa aagacagttc ttggggatct tttaaaacaa cgcgtgtcag tagtgatgta
    12361 tcacaagtag acttaaaaac tgctttgatc tattccgata atatatatgc ggcacaagaa
    12421 acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag ataaattcat ttttggtgaa
    12481 gaccttgatt tgccaattag tatgaatcca gcacaaattt ctaatgaaga tagctttaac
    12541 tcagatatct tgctagctga tactggatat ggacagggcg aacttctaat tactcctatc
    12601 cagcaagcag caatgtattc tgtttttacc aacaatggca cacttgtcta tcctaaattg
    12661 attgcagata aagagacaaa agataagaag aatgtcatcg gcgaaacagc agtacaaacg
    12721 atcgtgccag atctgagaga agttgtgcaa gatgtaaatg gtacagcaca ttctctttct
    12781 gctttaggga ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat
    12841 gtaaaggggc aagagaacag tttcttgttt gctttcaatc ttgataacca aggttatatg
    12901 atggtcagca tgttggaaaa taaagaagat gatgattcag caaccaaacg agcatcggaa
    12961 ctattgcaat acctcaacca aaattatcaa taaaaaattg ctttttaagt acagtaaaag
    13021 gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg atgtctcttt
    13081 cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat atttgagaac
    13141 aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat agtactgtgg
    13201 ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt ctccacgcga
    13261 aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa tctgagcacc
    13321 aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag aaataaagag
    13381 tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa aaaatactgg
    13441 tataaatact gcatatccta aagcctctac attgtggtat acttcttctt taacctttgt
    13501 ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc caacaagatc
    13561 cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag aagaatttgc
    13621 gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta ataagaaaat
    13681 aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat tgctagaagc
    13741 ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa tgtcatctac
    13801 cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta gttcttttaa
    13861 tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga aaatcgcttc
    13921 atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg ggaagatgat
    13981 cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag ataaatcact
    14041 ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt catggactag
    14101 aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa caagtaattg
    14161 tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg ttgttgaaat
    14221 caatatcagg cataaaatac caataaattc cattctcatc attcctttgt ataaaatata
    14281 gcccgaactt agcataacat acattattga aaaaatagga aaatatactc acaaaaaaac
    14341 caacaatcca aaagattttt ttggattgtt ggtttttata cggtctattt taataactaa
    14401 aaatgattaa cgtatagtca tttttcttat tctgatgaaa ttgataatct cctggatgcc
    14461 catgaaaatc aaaataaagc cgaatatttg gaatagaagc atcaaacttt tgaatggatt
    14521 gaacaataag acgaatccgc caatcaacaa cagaatacta ctataaacac tccaactttt
    14581 agcttttgta ttcatgctga agaacaactg gaacaagctg ttgatgataa cagctaatcc
    14641 aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa gcatagacga tcagagctaa
    14701 gatcaggaaa aaaagaccac tgaacagacc gatactccaa gaacctgttt tccttttaaa
    14761 acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca agataggcgg tgatcagata
    14821 accgataaaa tgaaaaacag cagaagggtt tagcacaatc gcaatcccag aaataatata
    14881 aacagcagct cttaataagg cgtatttttg aatattttgt ttaatcgtct caaacatata
    14941 agaaaacctc ctttgactta ttttaacgtg aactagtcat tttaccaaat taaatgttac
    15001 aaaatgagcg aaggagaaga atatctctgg aaaaatcaac ttctgaggat cttttccatt
    15061 tttttgcctt ttgctagctc atcgatcatt ttatctaaat aacgaatttc tttcatcaag
    15121 ttatcctcga tgtcttctac tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa
    15181 ggattgggga caggggcttc cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga
    15241 tattctagtt ctgtttggga ataacccgtc agccaacaaa tgatttcatc tacttcctct
    15301 tttatacgat cttttctctc cgcttttgct acatacatag gataaacgct tgcaaaactt
    15361 gtcgtaaaaa tacgattttg cgccataatc agtcaccttt ctttttttca aattatttac
    15421 tacttatagt atataagaaa aagagaagaa aaagcgatta ttcaaaaaaa caaaaaggat
    15481 tgtgacaaaa ttcttgtcac catccttttt tccgctctct gactttttac aatcttcata
    15541 gctcaagtgc gcatcagata atcgaatgct cctaaagctg cagtagctcc tgaacccatt
    15601 gaaacaatga tttgtttgta tggactatct gtacaatcac cagctgcgaa aacgccgggg
    15661 atattcgttg cgccattttt ctcggtcact atttcatttc ttggattcat ttcgatcgtt
    15721 ctatctagcc aatccgtatt agggagtaag ccgactagaa tgaatactcc tgaaacagct
    15781 agtgtatgca tctccatcgt acggcgatcg atataggaga gagattctac ctgattcgta
    15841 ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga cattcgcaag agagactagt
    15901 ttatcctgta agatttgatc tgcttttaat tctggtaaga attctagaac atatacatgt
    15961 tttgctaacc cagccaaatc gattgctgct tcaacaccag aattaccacc accgataaca
    16021 gcaatctctt ttcctttaaa taaagggccg tcacagtgtg ggcaataagc aattccttta
    16081 tttttaaact ctttttcgcc tggtacattg atagaacgcc agtgcgcacc tgtcgcgatg
    16141 atggcggttt ttgctttaag tgatataccg ttagctaagc caatattaat aaaatgttgc
    16201 tctttattta aggaaacggc ctgttgattc atcataatgt cgatagggta actacgcaca
    16261 tgactttcta cttgctgcat caattttgga ccttctgtgt aagaggtacc aatcatattt
    16321 tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat cagcgacaag tcctgtacgg
    16381 attcctttac gtgctgcata gatagctgca ctactagctg ctggtccacc gccaatcact
    16441 aatacatcat aaggttcttt ttcaaacaac tcttcttcaa tcaaagggcc agtgattttt
    16501 tcaacaattt gttcgatcgt catgcgtccg ctactaaatt cttctccatt taaaaacaca
    16561 gttggcactg ccatgatttt tttatcatcg atttcttttt ggaacatccc accttcaatc
    16621 atcgtatgag taatatttgg atttaataca gctaatatat tcaatgcctg tacaacgtcg
    16681 gggcaattat gacaagtcaa gctaacataa ctgtcaaaat gcaatgtttg tgttattttt
    16741 ttgattttaa gactcaaaga ttcactgatt ttcggtgccc gtccactaat ttgtaacata
    16801 gccagtaaaa aagaggaaaa ttcatgaccg agggggatac cagcaaaaac gattccactt
    16861 tctttgccaa ctggattgat agaaaaacta ggtgtgcgca tcgaatctct tttttcccaa
    16921 gagattttag gagacatttc tgctatttcc ttcgtaaatt cagctagttt ttgactattt
    16981 tcactattat ccaaactaac agaaaaaaca ataggagatt caatcagtgc taaatattgt
    17041 tcgagatttt gtttagtttc tttgtctaac atatccacca cctaaatctt tccaacaaga
    17101 tcaaaacttg gtttcaatgt ttcgccactt tctttccatt ttgccggaca aacttcacct
    17161 gggtgggtac gtacatattg agcagcgcga atcttatcaa tcagtgcact agcgtcacga
    17221 ccgattccat ctgcattgat ttccatagct tgaacgattc cgtctggatc aataataaat
    17281 gttcctcgct gagctaatcc ttgctcttca tccaaaacat caaaaatacg agaaatctga
    17341 tgagacggat caccgatcat cacgtattcg attttaccga tagcatctga tgtatcatgc
    17401 catgctttgt gagtaaagtg ggtatcggta gagcaagaaa agacttcaac acctaaagat
    17461 ttcaatgtac tgtattgttc ttgtaagtct tctaattcag taggacaaac aaaagtaaaa
    17521 tcagctggat agaagcaaag aatactccag ttccccataa gatctttttc actgacttct
    17581 aaaaattctc cttgatgata agcatgtgca ttaaattctt cgattttttt cccaatcaat
    17641 gacatagtaa aacctccaat aataattatt tatttataat tattgtaatt tagttggagt
    17701 tgcaaagtca atggatattt tgttatttaa taaagattac tcaaaaacga aggttaatta
    17761 taaaaataga agaggtcgca agaaaatgct actgctggaa atactcggga taacgttgag
    17821 taacagtttc ctgttctttc agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt
    17881 cttctggttt tttatcaact aaagccttga gtaaatcata atgttgttcc atgatcgttt
    17941 cccatggaag cccctgaatg gtcactcgta accagcggaa acgttctaaa tgggtattat
    18001 ggtcatctaa ccagttccaa acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa
    18061 ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt
    18121 cttctaaaat cgtttctagc actattcgtg tttgtgtgtc caatttggaa caacattcaa
    18181 tcatgatttg ctcttccaac tgttctcgga caaagcgagc attttctgca gaaacaagat
    18241 taattttgga aacatacgta ccactttgag gtatcgtata cacgagttct tgctgacgta
    18301 gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc aagatgttcc tccagtgcct
    18361 tttcagatat tttccttcca ggttctaagt cagcatagat aatttgttta cgaatcgctt
    18421 gataagcttg tgattgcaag ctgcttttca tctttattcc tccaaaatca tttctatgaa
    18481 cgtagtataa cagaagaggt ctgaaaaaca atctggtatg tcacattaac tagtaaattt
    18541 gtataaatag taaaattttg gttgacatag cgatttttat tgcataaaat tcacttgaat
    18601 acaaaaacta atatgttagt ttaggagggg aaaaatgatt gatttaaaca actggaggaa
    18661 ctttatccct gaattatctc aaaaagaagt agaaatacct cattttgacc gtgcatcatt
    18721 aagagaaaat ggacaaaaaa atcctgtctg gatccatttc ggtggcggaa atctttatcg
    18781 aggatttcat gcagaaatcg ctcaacgttt agcggatcta ggcgaattgt ccactggtgt
    18841 cattgtttgt gaaacattcg atgaagaaat aattgaaagg gcttaccaag attttgatga
    18901 tttgattctt gaagtggtca tgccaagcag tggagagttc gtcaaacgta tattagctgc
    18961 gacagctgac agtctgtatt gtcatccaga ttgttctgaa cactttgata agatgattcg
    19021 acactttgaa aatcctagtt tacaattagt tacttttgct attactgaaa aaggttatcg
    19081 attgaaagat acaaataata aatatatagg accgattgca gtcgatataa aaaatggtcc
    19141 aaagaagcca tcacatacaa tgagtattct tgctgtgcta ctttataaac gttttttatc
    19201 tggagcttgg ccgattgcac ttgtctctac agataatttt tcacaaaacg gtagtcaatt
    19261 acgtacttct ttgctaacga tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac
    19321 atttatccaa tacttatcga atgaagaaat tgttgcgttt ccatggacga tgattgatcg
    19381 tatcaccccg aatccttcct tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa
    19441 tatgtcgatt ttccaaaccg caaaaggaaa taatattgct ccatttgtca atacagagga
    19501 catccattat ttaatcatgg aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc
    19561 tggggtattc ttgactgatc gtgcaacggt agataaagca gatacgatga aggtgacgac
    19621 ttgccttaat cctttgcata cgtctttagc tattatgggt tgtttactgg ggtataggag
    19681 catagctgag gagatgaaag atccagattt atcgctgcta gtttctgaaa tcgggtacaa
    19741 tgaaggcctc ccagttgtgg agtctccagg tatcattgat ccaaagatct ttctggaaga
    19801 agtaattact gtgcgctttt ctaatccaaa tattcctaat atgcctcagc gaatcgccac
    19861 ggatacctct caaaaggtgg cgattcgtta tggtgagacg attaaaaaat atatggaaat
    19921 agaagttaaa gacccgcaga cacttgtatt cataccattg actctcgctg cttggcttcg
    19981 ataccttatt ggcattgatg atcaaggaaa accattcatc ccgagtccag acccactatt
    20041 gacagacctt cagcataagc tttccgatat tcaactgggt acgcaagata catcacatat
    20101 tcatgagaaa atggctccta ttttgtcaaa taaagaaatt tttggtgtag atctttacca
    20161 agcagagctt ggcagaaaaa tagaagaaat gttcatcaag atggtagcag gaccaaatgc
    20221 cgtacgaaaa acattacagg attacttaca agaacatgga ggaaatttat catggaaatg
    20281 acttttagat ggtacggatc acaggaagaa aagattacct tagatgaaat cagacagatt
    20341 cctgcggtca aagggattgt aggcacattg atggatatac cagtaggaga ggtatggcct
    20401 agggagagga tcaaagaact aaaagaagaa attgagtctc gtgggttaac actaaaagta
    20461 attgaaagcg tcaatgtgca tgatgccatt aaaattggat taccttcccg tgagcagtat
    20521 atcgaaaact ataagcagac aatccgaaac ttggcagaat atggaataga agtcatctgc
    20581 tataatttta tgcctgtatt tgactgggtg aaatctgacc ttgactaccg cttagaagac
    20641 ggctcatcca cgttagcttt tatatctgat gatattccag atgatccaag agaaatcgtc
    20701 gcgcgcatcg aacattccag caatgaattt ggattgccgg gatgggaacc tgagcgctta
    20761 gctcctatca aatccttgct tgaagcctat gcatcagtag acgaagaaaa acttcgagaa
    20821 aattttgcct attttctcca atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg
    20881 gctgtacatc cagatgatcc gccatactcg atttttggat tagctcgaat cgtaaagaat
    20941 cgagaagatt tagattggtt gtgtcgcata gtagacagcc cttataacgg aatcactctt
    21001 tgtacaggga gtattgcaga agatccagaa aatgatgtat atgggatttt agcagaattt
    21061 tgccaaagag atcgaatccc atttgcacat gttcgaaata ttaaattttt agctaatcga
    21121 gatttctatg aggcaccaca taaatccgaa tacggttcat tagacatgta ccgcatacta
    21181 aaaacaatgt atgacaacgg atttaacgga tacatccgtc ctgatcatgg aagaatgatc
    21241 tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg tgctgtttat
    21301 ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa
    21361 aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac ataaatccgg
    21421 aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt aacttgattt
    21481 tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata ttctgagatc
    21541 ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat atagattttt
    21601 ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt gcagttctcc
    21661 ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat cttaacgatc
    21721 agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat tcgcggctgt
    21781 tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac attttccttt
    21841 tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata aaattgttca
    21901 atggcggttt ggacttcttt caatacgcta tgttcttgat aaagcatgaa agtaggaagg
    21961 ggcgtatttg gttgattgaa gttaagaaat ccattataaa gtttatttcg tgcgaatgcc
    22021 aatgagtaaa gaatcttttc tttttcaggt ataaacaaat caaatacagt acaaagggac
    22081 tccaccattt ctcttgaata aagaacatga ggaacgtttg ctttttttaa aagtttgtat
    22141 tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag aaagtcgagc taaaatattc
    22201 actaagatgc gttgttcgcg acaaattggc agattaggtc gcaaaatatc tacagagaga
    22261 tcaataaaaa agtcaatata ttcatactct gtgaattctt tttgtacgtc tttcacagaa
    22321 gttgcaggaa aattctcttt acatattttt aatagaaaat ataatttatc tatcataccg
    22381 ctatttagtc ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact
    22441 tgcggatctt catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct
    22501 aatgctgcat attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa
    22561 aaggagatag acaaaccgta ctatgctaaa aatgcattaa tctttttcat tttcgtatac
    22621 aaataagagg cactatagtt tgtttgctga ctgaaactga taatcgaatt tgtctgacag
    22681 ctgaacaaag aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga
    22741 cgataatagc agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc
    22801 ttatcaatat gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg
    22861 tatagtaaaa aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata
    22921 aagaaacgat gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc
    22981 aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat
    23041 tgttaaaact tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca
    23101 tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag
    23161 ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta
    23221 taaatcagac agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg
    23281 ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa
    23341 aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag
    23401 cacctatact acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct
    23461 atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc accgttacat
    23521 taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga
    23581 acgatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg
    23641 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
    23701 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
    23761 aacccgatac agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg
    23821 gagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg
    23881 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag
    23941 aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag
    24001 aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc
    24061 tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg
    24121 cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa
    24181 aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat
    24241 cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct
    24301 ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat
    24361 gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc
    24421 atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt
    24481 caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa
    24541 gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct
    24601 ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat
    24661 caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat
    24721 acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa
    24781 actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa
    24841 aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc
    24901 aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa
    24961 tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa
    25021 cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg
    25081 aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc
    25141 tgcatctttt ggctgatgct cgtaaaaatc aataacggac tggatcatat caaatgtttg
    25201 atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt
    25261 ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac
    25321 aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct
    25381 aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt
    25441 cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac
    25501 gttattcatt atatgatgga atatgaaaat aagaaagaga aagagcaaaa agaaaaaccg
    25561 ctaaatgacg ttatcttatg agttggtttt cctcataaaa tcgttcatct agcagtttca
    25621 gccctttttc tggtcttgat gaaaattatc tagcattttt cgggtcagat gttgcagtgt
    25681 atccaattct tcctgttcaa gtggaagata agacttgatc ttttgtggaa taccttctgc
    25741 ttgtttttgc aacgcagcac cttgaggagt cagcgtaaca tttacgattc tttcatcacc
    25801 cgtttcacgc tgacgattaa tataattttt ttctgctaac ttttttagta agggagtcag
    25861 cgtaccagaa tccagacaca aatcatttcc tagctctttc actgaagtct ttttcttttc
    25921 ccataaaact aacatcacaa gatactgggt ataggtaaga ttcagcggct tgaggaggga
    25981 agtgtagtgt ctaatgattt ccttagaggc ggcatataaa ggaaaacata cttgattaac
    26041 taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat catagcggat
    26101 ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt tccagctgta
    26161 gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt ttcttccaga
    26221 gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt ttcactttct
    26281 gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt ttgctgtttg
    26341 atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg
    26401 ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa tgtacgatcg
    26461 ggattatgaa cttctccaga gcgtccgcct gtgttgatca ttttcgtaga atacattttt
    26521 ttcataaaat taccaccttt aattatattt tatacaattg aattttacac aatataattg
    26581 tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg aaacagacgc
    26641 gctttctcga atagcaaaca tatacaaaaa aatatcagag acaaaaacta atctcatcat
    26701 aaacattaca tatatttctc aaaaagttgt tttctttctt caaaacttac ttcacgatgg
    26761 acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt agtcacacca
    26821 aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa tgctttatta
    26881 tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg atgttcagca
    26941 gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct cgtgccatac
    27001 aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat cagttgtatt
    27061 tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa tacaaaatct
    27121 atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag ttactagatt
    27181 cttttattat accagttaaa gaaaacgcat tcaaaaaaat agcggattta cttaccaact
    27241 cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct gctgacaatg
    27301 acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa ttcatgatca
    27361 gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt tgcctctagc
    27421 agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc caaataatgt
    27481 tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata agtcatgaca
    27541 attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc aaaaacaaac
    27601 agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt attcaagtgg
    27661 aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga gttgttttta
    27721 tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga caacaaccga
    27781 tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaaa caagcttccg
    27841 aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac caacataacg
    27901 atattcaaca ctaaaataac tatagctcgg tcaaatccta aaacagcttc cagtaatatt
    27961 cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat attgatagcg
    28021 actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt
    28081 tctttcattt atccacttcc ttaattcatt aataatactc ttatcttatt ataacttact
    28141 tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat tattatgttt
    28201 cattcttcta gaatatactt ctacgttaaa aactgttaaa aaacttttac tttagaaaat
    28261 tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga agtagattaa
    28321 aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc
    28381 aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgtttctct atcacttata
    28441 gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat
    28501 cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt
    28561 actaggaaaa atgatatcaa aaaagtatat gtgctttgtg gagatccata taccataaca
    28621 actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat
    28681 gattattggg aaattgaagt gacgagcgaa acaaggcgct tacagtatgc gttccatgtt
    28741 gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa
    28801 aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga
    28861 ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt
    28921 gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca
    28981 gaccgagaag acttcttcgg gggagatttg caaggtgtga gggatcattt ggattacttg
    29041 acggaccttg gcgtcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat
    29101 aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta
    29161 aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc
    29221 aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg
    29281 atctataaag actggttcca catccgtttt ttcccagtag attcttatca gatgactgac
    29341 ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg
    29401 aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg
    29461 ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat
    29521 ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga
    29581 gagatttggc attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat
    29641 tatgcattca cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa
    29701 atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca
    29761 ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa
    29821 gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat
    29881 tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata
    29941 tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta
    30001 aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat agtatatgat
    30061 cgtgaagata agctctattt cacacgggaa ctagagggac agatcatcta tgtttacttt
    30121 aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat
    30181 tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt
    30241 tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat
    30301 aataagggtg taacagcacg ttgaatacac aggaggaaat gaagatgaaa aaaagaagtg
    30361 tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg
    30421 gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg
    30481 ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg
    30541 atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag
    30601 atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt
    30661 taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa
    30721 cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga
    30781 cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt
    30841 tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag
    30901 gatttttagc taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg
    30961 gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg
    31021 ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc
    31081 aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag
    31141 caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg
    31201 tagccaaaat tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag
    31261 gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg
    31321 taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc
    31381 aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac
    31441 aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg
    31501 cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg
    31561 aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt
    31621 ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa
    31681 attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg
    31741 gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg
    31801 ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt
    31861 tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt
    31921 aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga
    31981 aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt
    32041 agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa
    32101 ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta
    32161 tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac
    32221 taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa
    32281 cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg
    32341 gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc
    32401 ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt
    32461 accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag
    32521 cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat
    32581 cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca
    32641 gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc
    32701 agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa
    32761 catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg
    32821 gaatttcaat aatttctcca tgatctatct gtttaacaat ggtggtcccg gaagcgtagg
    32881 cggaggagct ggttctactg atattcttat ttcctggatc tataaactta cgactggaac
    32941 ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg
    33001 tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa
    33061 aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt
    33121 cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc
    33181 aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac
    33241 tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt
    33301 tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt
    33361 cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta
    33421 ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat
    33481 aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg
    33541 atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg
    33601 gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc
    33661 gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg
    33721 cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg
    33781 acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt
    33841 ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa
    33901 ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg
    33961 ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt
    34021 gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa
    34081 gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa
    34141 aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct
    34201 gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga
    34261 acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata
    34321 cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa
    34381 gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta
    34441 tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg
    34501 gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa
    34561 gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta
    34621 gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg
    34681 ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt
    34741 taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa
    34801 tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg
    34861 gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca
    34921 cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga
    34981 gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag
    35041 attacaagta acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc
    35101 gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga
    35161 ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa
    35221 gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca
    35281 actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa
    35341 atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt
    35401 gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag
    35461 gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa
    35521 gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa
    35581 acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa
    35641 ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga
    35701 atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc
    35761 ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg
    35821 attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat
    35881 tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg
    35941 gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa
    36001 tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa
    36061 aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac
    36121 ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca
    36181 taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact
    36241 tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta
    36301 tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac
    36361 atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac
    36421 aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc
    36481 agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac
    36541 tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg
    36601 tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac
    36661 agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg
    36721 tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt
    36781 aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta cttattggga
    36841 agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga
    36901 agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc
    36961 ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta
    37021 gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac
    37081 tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact
    37141 aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct
    37201 ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat
    37261 gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc
    37321 gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat
    37381 gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac
    37441 gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta
    37501 ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg
    37561 aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc
    37621 gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga
    37681 gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca
    37741 gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg
    37801 ctatgtggcg caagcatgtt acagattggt acagagcttc ataaagaagg gccggaaatt
    37861 ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt
    37921 gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga
    37981 tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg
    38041 tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg
    38101 caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg
    38161 atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac
    38221 cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg
    38281 gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag
    38341 gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag
    38401 aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata
    38461 ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg
    38521 gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg
    38581 ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga
    38641 aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg
    38701 ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga
    38761 gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa
    38821 gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg
    38881 aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt
    38941 tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga
    39001 atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca
    39061 gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg
    39121 ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc
    39181 tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca
    39241 gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg
    39301 aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt
    39361 atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa
    39421 aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat
    39481 ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat
    39541 agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc
    39601 cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag
    39661 taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc
    39721 ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc
    39781 taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc
    39841 tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga
    39901 aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa
    39961 aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga
    40021 acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga
    40081 caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct
    40141 aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat
    40201 actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga
    40261 tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa
    40321 gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc
    40381 tgagtcggtg agtgagtgat gtactttccc ctaattgatt tttccctcat gtggacttca
    40441 cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt
    40501 ttaggaaatt tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg
    40561 aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg
    40621 tttctgttat atttcgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc
    40681 tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt
    40741 tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa
    40801 tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg
    40861 gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa
    40921 gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg
    40981 atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct
    41041 atccatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt
    41101 tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga
    41161 aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt
    41221 tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg
    41281 accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc
    41341 gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg
    41401 ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa
    41461 tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg
    41521 cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag
    41581 aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa
    41641 tggagcgagg cgtaaaatat tttccaaaag atcaattgta caaagaattg aaaagacgtt
    41701 cagaaaatca agatccattt tctaacgaac gatattatga acggttttta gaggaaatag
    41761 ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat
    41821 atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat
    41881 gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc
    41941 gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac
    42001 gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa
    42061 tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga
    42121 aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga
    42181 ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg
    42241 gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat
    42301 ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga
    42361 aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga
    42421 cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt
    42481 ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt
    42541 gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga
    42601 agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc
    42661 aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt
    42721 gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa
    42781 agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga
    42841 taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga
    42901 aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc
    42961 aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata
    43021 ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat
    43081 tagtctcagg cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg
    43141 gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag
    43201 caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc
    43261 gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct gctggattca
    43321 cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa
    43381 tcatcaattg actataaaat catctgagca aagataaaga cattaacact actgaaaaaa
    43441 acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag
    43501 aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg
    43561 ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga tctctcggct
    43621 tatcctacga gtattattcg atacatttat atcaataatc ctttttttat tttatctgca
    43681 gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt
    43741 ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa
    43801 tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga
    43861 cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg
    43921 taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt
    43981 tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg
    44041 tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg
    44101 taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa
    44161 ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa
    44221 caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt
    44281 attttatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag
    44341 caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat
    44401 cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat
    44461 aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac
    44521 aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca
    44581 tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt
    44641 gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat
    44701 aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat
    44761 ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa
    44821 tacataggga tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc
    44881 agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac
    44941 tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc
    45001 cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata
    45061 agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa
    45121 taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg
    45181 catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa
    45241 gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac
    45301 aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa
    45361 catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag
    45421 ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat cactcctatt
    45481 ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt
    45541 cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata
    45601 aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa
    45661 gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt
    45721 cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat
    45781 catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat
    45841 cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt
    45901 cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg
    45961 tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta
    46021 ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga
    46081 tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg
    46141 cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta
    46201 aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt
    46261 ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt
    46321 ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag
    46381 attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg
    46441 tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat
    46501 cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat
    46561 gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc
    46621 ctttttggct ttcatgatac agttggttca agcaagtagc gagctttcca cttcctgggc
    46681 caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac
    46741 catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat
    46801 agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac
    46861 tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta
    46921 accgcaagat atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa
    46981 tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt
    47041 tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta
    47101 acttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg
    47161 ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc
    47221 cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt
    47281 cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat
    47341 gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat
    47401 aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg
    47461 gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag
    47521 cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg
    47581 gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa
    47641 ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag
    47701 aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag
    47761 tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaacgc
    47821 ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat
    47881 gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga
    47941 tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc
    48001 aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca
    48061 ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat
    48121 cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt
    48181 tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta
    48241 tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg
    48301 tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg
    48361 attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt
    48421 actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca
    48481 gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga
    48541 aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat
    48601 ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg
    48661 aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag
    48721 aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca
    48781 cagaagaaat ccctatgcta gaggaaacaa aaatacatga aaaagaaaaa gtaactccga
    48841 cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt
    48901 tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag
    48961 aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag
    49021 aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc
    49081 tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt
    49141 caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg
    49201 gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg
    49261 aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc
    49321 gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca
    49381 cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg
    49441 ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg
    49501 aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg
    49561 atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa
    49621 cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac
    49681 gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt
    49741 cctgattgga taagttggat taccagatgt cattcagata attctgttcc aaaggacttg
    49801 cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat
    49861 aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta
    49921 gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc
    49981 agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa
    50041 ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca
    50101 tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa
    50161 aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt
    50221 acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct
    50281 attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc
    50341 ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa
    50401 ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca
    50461 acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca
    50521 atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt caaaaaaggc
    50581 gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc
    50641 acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg
    50701 gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc
    50761 attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca
    50821 taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc
    50881 ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt
    50941 gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt
    51001 gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc
    51061 gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg
    51121 atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat
    51181 ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta
    51241 atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa
    51301 ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg
    51361 gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat
    51421 gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg
    51481 ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca
    51541 gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc
    51601 gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc
    51661 gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt
    51721 cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat
    51781 tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct
    51841 tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta
    51901 tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa
    51961 gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga
    52021 gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc
    52081 ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca
    52141 acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt
    52201 gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat
    52261 gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga
    52321 aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt
    52381 aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc
    52441 atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta
    52501 ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt
    52561 gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa
    52621 aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg
    52681 gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt
    52741 catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc
    52801 atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt
    52861 taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc
    52921 aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac
    52981 aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca
    53041 gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt
    53101 ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc
    53161 ttttttattt taggaattag agagggattt gtccttctat gattttttgc tataataggg
    53221 aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca
    53281 aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt
    53341 gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta
    53401 taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa
    53461 tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct
    53521 gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat
    53581 gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat
    53641 actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag
    53701 aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca
    53761 ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg
    53821 aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc
    53881 gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt
    53941 gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga
    54001 aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt
    54061 aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt
    54121 cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt
    54181 aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat
    54241 acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc
    54301 attgactgga atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc
    54361 tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac
    54421 gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt
    54481 agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata
    54541 cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt
    54601 tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac
    54661 tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca
    54721 gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat
    54781 tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa
    54841 tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg
    54901 aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat
    54961 atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat
    55021 taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa
    55081 gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa
    55141 cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt
    55201 tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga
    55261 gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa
    55321 cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa
    55381 gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg
    55441 tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata cctatgctta
    55501 tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc
    55561 gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt
    55621 tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg
    55681 tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt
    55741 agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga
    55801 aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc
    55861 catttcagcg caataattcc ttttattcaa aaaacatacc aaaaata
//
LOCUS       DACECS010000014        52140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.014, whole genome shotgun sequence.
ACCESSION   DACECS010000014 DACECS010000000
VERSION     DACECS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.014"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWO31_000881"
     CDS             330..1175
                     /locus_tag="IWO31_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0301315.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWO31_000882"
     CDS             1456..2676
                     /locus_tag="IWO31_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0301316.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWO31_000883"
     CDS             2673..5393
                     /locus_tag="IWO31_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0301317.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWO31_000884"
     CDS             5377..6321
                     /locus_tag="IWO31_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0301318.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWO31_000885"
     CDS             complement(6390..7400)
                     /locus_tag="IWO31_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0301319.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWO31_000886"
     CDS             complement(7580..7924)
                     /locus_tag="IWO31_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0301320.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWO31_000887"
     CDS             complement(7917..8351)
                     /locus_tag="IWO31_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0301321.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWO31_000888"
     CDS             8489..9226
                     /locus_tag="IWO31_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301322.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWO31_000889"
     CDS             9219..10430
                     /locus_tag="IWO31_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301323.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWO31_000890"
     CDS             10637..11416
                     /locus_tag="IWO31_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0301324.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWO31_000891"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWO31_000891"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0301325.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO31_000892"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO31_000892"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0301326.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO31_000893"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO31_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0301327.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWO31_000894"
     CDS             15018..16067
                     /locus_tag="IWO31_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0301328.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWO31_000895"
     CDS             16064..16342
                     /locus_tag="IWO31_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0301329.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWO31_000896"
     CDS             16317..16787
                     /locus_tag="IWO31_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0301330.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IWO31_000897"
     CDS             16804..17106
                     /locus_tag="IWO31_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301331.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IWO31_000898"
     CDS             17123..17494
                     /locus_tag="IWO31_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0301332.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWO31_000899"
     CDS             17466..17825
                     /locus_tag="IWO31_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301333.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWO31_000900"
     CDS             17997..19004
                     /locus_tag="IWO31_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0301334.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWO31_000901"
     CDS             19029..20213
                     /locus_tag="IWO31_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0301335.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWO31_000902"
     CDS             complement(20650..22233)
                     /locus_tag="IWO31_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0301336.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWO31_000903"
     CDS             complement(22811..22954)
                     /locus_tag="IWO31_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0301337.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IWO31_000904"
     CDS             complement(23176..23655)
                     /locus_tag="IWO31_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0301338.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IWO31_000905"
     CDS             complement(23728..24012)
                     /locus_tag="IWO31_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301339.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IWO31_000906"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO31_000906"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IWO31_000907"
     CDS             complement(24356..25642)
                     /locus_tag="IWO31_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0301340.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IWO31_000908"
     CDS             25780..26451
                     /locus_tag="IWO31_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0301341.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IWO31_000909"
     CDS             26417..26905
                     /locus_tag="IWO31_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0301342.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IWO31_000910"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IWO31_000910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0301343.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IWO31_000911"
     CDS             27866..28630
                     /locus_tag="IWO31_000911"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0301344.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IWO31_000912"
     CDS             28863..31076
                     /locus_tag="IWO31_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0301345.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IWO31_000913"
     CDS             31087..31533
                     /locus_tag="IWO31_000913"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0301346.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IWO31_000914"
     CDS             31704..32963
                     /locus_tag="IWO31_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0301347.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IWO31_000915"
     CDS             complement(33052..33999)
                     /locus_tag="IWO31_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0301348.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IWO31_000916"
     CDS             34393..35685
                     /locus_tag="IWO31_000916"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0301349.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IWO31_000917"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IWO31_000917"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0301350.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IWO31_000918"
     CDS             37578..38453
                     /locus_tag="IWO31_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0301351.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IWO31_000919"
     CDS             38719..40602
                     /locus_tag="IWO31_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0301352.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IWO31_000920"
     CDS             complement(40677..40961)
                     /locus_tag="IWO31_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0301353.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IWO31_000921"
     CDS             complement(40951..41685)
                     /locus_tag="IWO31_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0301354.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IWO31_000922"
     CDS             41814..42437
                     /locus_tag="IWO31_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0301355.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IWO31_000923"
     CDS             42629..43750
                     /locus_tag="IWO31_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0301356.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IWO31_000924"
     CDS             43757..46885
                     /locus_tag="IWO31_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0301357.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IWO31_000925"
     CDS             46904..50098
                     /locus_tag="IWO31_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0301358.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IWO31_000926"
     CDS             complement(50244..50951)
                     /locus_tag="IWO31_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0301359.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="IWO31_000927"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="IWO31_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0301360.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17114 a         9064 c        10788 g        15174 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DACECS010000015        51635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.015, whole genome shotgun sequence.
ACCESSION   DACECS010000015 DACECS010000000
VERSION     DACECS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.015"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IWO31_000928"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IWO31_000928"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0301361.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IWO31_000929"
     CDS             1281..1727
                     /locus_tag="IWO31_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0301362.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="IWO31_000930"
     CDS             2000..3352
                     /locus_tag="IWO31_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0301363.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="IWO31_000931"
     CDS             3523..3810
                     /locus_tag="IWO31_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301364.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="IWO31_000932"
     CDS             4036..4479
                     /locus_tag="IWO31_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0301365.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="IWO31_000933"
     CDS             complement(4485..5669)
                     /locus_tag="IWO31_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0301366.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="IWO31_000934"
     CDS             5937..6635
                     /locus_tag="IWO31_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0301367.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="IWO31_000935"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="IWO31_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0301368.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="IWO31_000936"
     CDS             complement(7445..7978)
                     /locus_tag="IWO31_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301369.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="IWO31_000937"
     CDS             complement(8275..10656)
                     /locus_tag="IWO31_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0301370.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO31_000938"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO31_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0301371.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="IWO31_000939"
     CDS             11357..11896
                     /locus_tag="IWO31_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0301372.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO31_000940"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO31_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0301373.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="IWO31_000941"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO31_000941"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="IWO31_000942"
     CDS             13003..14169
                     /locus_tag="IWO31_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0301374.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="IWO31_000943"
     CDS             14215..15711
                     /locus_tag="IWO31_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0301375.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="IWO31_000944"
     CDS             15792..16475
                     /locus_tag="IWO31_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0301376.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="IWO31_000945"
     CDS             17120..18310
                     /locus_tag="IWO31_000945"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0301377.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="IWO31_000946"
     CDS             18412..19365
                     /locus_tag="IWO31_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0301378.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNVLEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="IWO31_000947"
     CDS             19434..20783
                     /locus_tag="IWO31_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0301379.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20966..21289)
                     /locus_tag="IWO31_000948"
     CDS             complement(20966..21289)
                     /locus_tag="IWO31_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301380.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21418..22581
                     /locus_tag="IWO31_000949"
     CDS             21418..22581
                     /locus_tag="IWO31_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301381.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22651..24000
                     /locus_tag="IWO31_000950"
     CDS             22651..24000
                     /locus_tag="IWO31_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0301382.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24045..24476)
                     /locus_tag="IWO31_000951"
     CDS             complement(24045..24476)
                     /locus_tag="IWO31_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301383.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24864..27506
                     /gene="alaS"
                     /locus_tag="IWO31_000952"
     CDS             24864..27506
                     /gene="alaS"
                     /locus_tag="IWO31_000952"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0301384.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27706..28302
                     /locus_tag="IWO31_000953"
     CDS             27706..28302
                     /locus_tag="IWO31_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0301385.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28424..29572
                     /locus_tag="IWO31_000954"
     CDS             28424..29572
                     /locus_tag="IWO31_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0301386.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29610..30023)
                     /locus_tag="IWO31_000955"
     CDS             complement(29610..30023)
                     /locus_tag="IWO31_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0301387.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30102..30533
                     /locus_tag="IWO31_000956"
     CDS             30102..30533
                     /locus_tag="IWO31_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0301388.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30632..30865)
                     /locus_tag="IWO31_000957"
     CDS             complement(30632..30865)
                     /locus_tag="IWO31_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0301389.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31048..32718
                     /locus_tag="IWO31_000958"
     CDS             31048..32718
                     /locus_tag="IWO31_000958"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0301390.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32865..33251
                     /locus_tag="IWO31_000959"
     CDS             32865..33251
                     /locus_tag="IWO31_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301391.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33238..33936
                     /locus_tag="IWO31_000960"
     CDS             33238..33936
                     /locus_tag="IWO31_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0301392.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33941..34405
                     /locus_tag="IWO31_000961"
     CDS             33941..34405
                     /locus_tag="IWO31_000961"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0301393.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34402..35313
                     /locus_tag="IWO31_000962"
     CDS             34402..35313
                     /locus_tag="IWO31_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0301394.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35548..36087
                     /gene="pyrR"
                     /locus_tag="IWO31_000963"
     CDS             35548..36087
                     /gene="pyrR"
                     /locus_tag="IWO31_000963"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0301395.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36101..37384
                     /locus_tag="IWO31_000964"
     CDS             36101..37384
                     /locus_tag="IWO31_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0301396.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37397..38323
                     /locus_tag="IWO31_000965"
     CDS             37397..38323
                     /locus_tag="IWO31_000965"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0301397.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38342..39622
                     /locus_tag="IWO31_000966"
     CDS             38342..39622
                     /locus_tag="IWO31_000966"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0301398.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39627..40703
                     /gene="carA"
                     /locus_tag="IWO31_000967"
     CDS             39627..40703
                     /gene="carA"
                     /locus_tag="IWO31_000967"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0301399.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40708..43893
                     /gene="carB"
                     /locus_tag="IWO31_000968"
     CDS             40708..43893
                     /gene="carB"
                     /locus_tag="IWO31_000968"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0301400.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43947..44723
                     /locus_tag="IWO31_000969"
     CDS             43947..44723
                     /locus_tag="IWO31_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0301401.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44720..45652
                     /locus_tag="IWO31_000970"
     CDS             44720..45652
                     /locus_tag="IWO31_000970"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0301402.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45649..46365
                     /gene="pyrF"
                     /locus_tag="IWO31_000971"
     CDS             45649..46365
                     /gene="pyrF"
                     /locus_tag="IWO31_000971"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0301403.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46372..47061
                     /locus_tag="IWO31_000972"
     CDS             46372..47061
                     /locus_tag="IWO31_000972"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0301404.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47034..47303
                     /locus_tag="IWO31_000973"
     CDS             47034..47303
                     /locus_tag="IWO31_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301405.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47341..47982
                     /locus_tag="IWO31_000974"
     CDS             47341..47982
                     /locus_tag="IWO31_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0301406.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48013..48717
                     /locus_tag="IWO31_000975"
     CDS             48013..48717
                     /locus_tag="IWO31_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0301407.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            48714..49616
                     /locus_tag="IWO31_000976"
     CDS             48714..49616
                     /locus_tag="IWO31_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0301408.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49774..51480)
                     /locus_tag="IWO31_000977"
     CDS             complement(49774..51480)
                     /locus_tag="IWO31_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0301409.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16894 a         8982 c        11096 g        14663 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgtatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct
    20821 gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt
    20881 taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa
    20941 atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta
    21001 cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca
    21061 gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa
    21121 gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga
    21181 gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa
    21241 ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc
    21301 ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct
    21361 tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg
    21421 gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg
    21481 atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg
    21541 gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta
    21601 ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta
    21661 tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc
    21721 tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc
    21781 ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt
    21841 gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc
    21901 tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga
    21961 atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg
    22021 gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat
    22081 ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt
    22141 gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta
    22201 tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt
    22261 atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac
    22321 gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc
    22381 atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc
    22441 ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg
    22501 attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag
    22561 gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt
    22621 cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa
    22681 ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa
    22741 cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca
    22801 ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa
    22861 gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct
    22921 acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca
    22981 gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg
    23041 cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca
    23101 tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa
    23161 attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa
    23221 aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca
    23281 aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag
    23341 aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc
    23401 aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta
    23461 gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag
    23521 aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa
    23581 ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt
    23641 gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat
    23701 gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc
    23761 aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca
    23821 aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag
    23881 cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa
    23941 atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa
    24001 aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt
    24061 ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct
    24121 tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct
    24181 tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg
    24241 tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa
    24301 cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata
    24361 tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat
    24421 ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata
    24481 ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa
    24541 tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata
    24601 gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa
    24661 attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg
    24721 gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt
    24781 gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta
    24841 atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt
    24901 ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct ttgatccccg
    24961 tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg
    25021 atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa
    25081 atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta
    25141 atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga
    25201 ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag
    25261 atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg
    25321 atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt
    25381 tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag
    25441 gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata
    25501 agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg
    25561 aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc
    25621 ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa
    25681 cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg
    25741 acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg
    25801 ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg
    25861 ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg
    25921 aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa
    25981 tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag
    26041 acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag
    26101 aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg
    26161 aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga
    26221 cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat
    26281 tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa
    26341 tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat
    26401 ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta
    26461 atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc
    26521 atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc
    26581 tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag
    26641 tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat
    26701 tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa
    26761 ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat
    26821 acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca
    26881 cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg
    26941 gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag
    27001 aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag
    27061 ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac
    27121 aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga
    27181 tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc
    27241 aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac
    27301 aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa
    27361 aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc
    27421 cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg
    27481 cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa
    27541 acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt
    27601 tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag
    27661 tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat
    27721 aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg
    27781 gttccttggc tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca
    27841 gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg
    27901 ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg
    27961 ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg
    28021 gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg
    28081 gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca
    28141 ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg
    28201 gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta
    28261 gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt
    28321 aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca
    28381 tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga
    28441 agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt
    28501 aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg
    28561 aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt
    28621 gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca
    28681 cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt
    28741 tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc
    28801 catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta
    28861 tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta
    28921 taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg
    28981 atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt atagtggatt
    29041 gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat
    29101 tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt
    29161 ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc
    29221 aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc
    29281 tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta
    29341 taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca
    29401 tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa
    29461 attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat
    29521 cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt aaatgagaaa
    29581 ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt taattgttta
    29641 tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg caaccattgc
    29701 aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta gggaaggaag
    29761 agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa
    29821 cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag
    29881 acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt
    29941 ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag
    30001 gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat
    30061 ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat
    30121 ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt
    30181 ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag
    30241 gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact
    30301 ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc
    30361 gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat
    30421 ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg
    30481 gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa
    30541 aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca
    30601 tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct
    30661 gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa
    30721 attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat
    30781 gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa
    30841 accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc
    30901 ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt
    30961 ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga
    31021 ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca
    31081 gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa
    31141 ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac
    31201 gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag
    31261 tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt
    31321 gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga
    31381 ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat
    31441 gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga
    31501 aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat
    31561 cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa
    31621 gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac
    31681 cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag
    31741 cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca
    31801 atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg
    31861 tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta
    31921 gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta
    31981 gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc
    32041 cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat
    32101 gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag
    32161 ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg
    32221 ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat
    32281 ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa
    32341 gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc
    32401 gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata
    32461 gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat
    32521 tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc
    32581 gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg
    32641 atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa
    32701 gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta
    32761 cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc attctttttc
    32821 tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta
    32881 taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa
    32941 aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga
    33001 tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg
    33061 cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa
    33121 ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt
    33181 tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg
    33241 gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag
    33301 atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa
    33361 caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg
    33421 atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc
    33481 aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt
    33541 gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta
    33601 gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt
    33661 actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta
    33721 acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt
    33781 gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg
    33841 gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga
    33901 cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat
    33961 attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct
    34021 agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa
    34081 cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact
    34141 cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg
    34201 gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat
    34261 tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa
    34321 cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga
    34381 tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt
    34441 aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg
    34501 tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta
    34561 aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca
    34621 gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct
    34681 caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg
    34741 atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac
    34801 cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa
    34861 tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat
    34921 ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac
    34981 cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg
    35041 gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag
    35101 atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg
    35161 aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac
    35221 ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact
    35281 ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa
    35341 cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg
    35401 ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc
    35461 agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt
    35521 ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc
    35581 atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa
    35641 gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa
    35701 cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac
    35761 cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt
    35821 tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt
    35881 cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc
    35941 ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt
    36001 ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc
    36061 atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt
    36121 tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg
    36181 attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt
    36241 aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt
    36301 tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat
    36361 gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg
    36421 attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa
    36481 aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc
    36541 aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt
    36601 agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg
    36661 attgattccg attttactag gtattattag cggatacatc gtagccttgc tgtttggaat
    36721 cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc
    36781 ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc
    36841 ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg
    36901 taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat
    36961 cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct
    37021 ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat
    37081 tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc
    37141 aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga
    37201 gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt
    37261 aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc
    37321 aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa
    37381 ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac
    37441 cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa
    37501 gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga
    37561 aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt
    37621 gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt
    37681 actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta
    37741 ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg
    37801 aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg
    37861 tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa
    37921 atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga
    37981 atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga
    38041 tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag
    38101 tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt
    38161 gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga
    38221 ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt
    38281 cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa
    38341 aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc
    38401 agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga
    38461 atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca
    38521 tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc
    38581 agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga
    38641 tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt
    38701 attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc
    38761 actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg
    38821 gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac
    38881 agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact
    38941 tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct
    39001 agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg
    39061 tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt caccccatca
    39121 tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa tgaacccgcc
    39181 gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg gtacgatcga
    39241 ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa gtttcatgaa
    39301 atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata ctcattttgt
    39361 agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta aaccggctga
    39421 gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg tagcagtttt
    39481 tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag gggagaatac
    39541 accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca acggaaaact
    39601 tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa gatggaacag
    39661 ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc gtttttacaa
    39721 caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt caaatcatta
    39781 catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat gaatcgattt
    39841 ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca aactggcgaa
    39901 accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata tcaggtatcg
    39961 atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct agtatagttg
    40021 atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt ctagcaacca
    40081 atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca ggtcggaaag
    40141 tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt aaaagaaact
    40201 gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg ctatcaccag
    40261 acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa gcaatcgaaa
    40321 tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt caccagttat
    40381 ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga ggattaaatc
    40441 acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat catggctatg
    40501 cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta gaagtaaatg
    40561 acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg cagtttcatc
    40621 cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt atggaaatga
    40681 tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa gaaaattatg
    40741 gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta cgcaggaaca
    40801 caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa ttctaatcct
    40861 gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc gatcacactt
    40921 gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc aactttaggc
    40981 ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt agaagaattg
    41041 aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga tcgtgatcta
    41101 ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat cgtcaataca
    41161 gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt tcgtccagcc
    41221 tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt acgtatcgta
    41281 tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg aagtattgca
    41341 ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc aatcgttgtc
    41401 tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat cgtatttgcc
    41461 cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc tctttcgatt
    41521 attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga tccacatagt
    41581 tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc gttagcaagt
    41641 aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg attaacatta
    41701 gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc tgcattagat
    41761 tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg agaacggctt
    41821 ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa tatcgaagag
    41881 tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga acttcctgag
    41941 ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca agatgatcgc
    42001 ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt agcggatttg
    42061 acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat cgaacagcag
    42121 ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa cggttttaca
    42181 gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca gatgagaaaa
    42241 gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga attcgaatca
    42301 aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg atcagaaaag
    42361 ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt tgaatttgac
    42421 tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc aatcatcatg
    42481 aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt atattttgag
    42541 ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat aggagtaatc
    42601 gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc tgctggtgta
    42661 aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga tttattcgag
    42721 caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac aagtaaagaa
    42781 gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc aagctatgta
    42841 ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga ttacatggaa
    42901 catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt gcttggtggt
    42961 gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg gatcatggag
    43021 catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc acctcaaaca
    43081 ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc attaggtttg
    43141 aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt ttatgtgatc
    43201 gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac aggaattcca
    43261 atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact tggttatgaa
    43321 aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt ttcttttacg
    43381 aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg agaagtaatg
    43441 ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc tggattacac
    43501 ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga agaagcacta
    43561 gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa acatacagct
    43621 gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc tgaggaagca
    43681 gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt tgtcaatacg
    43741 attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag agaagcagtt
    43801 gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt tcgagtgatg
    43861 gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa gacaaaaaga
    43921 cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc gttcagcaaa
    43981 aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta aaagaaatgc
    44041 agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta ttacgccgac
    44101 cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt tatcgggtgg
    44161 aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta gatgtcatgg
    44221 gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa gtatttataa
    44281 tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta gtcgaaaaag
    44341 gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat tatgaaaaag
    44401 aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac ggtatgcacg
    44461 gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt tttgcatgtg
    44521 gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa aatgtccagt
    44581 tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc gtttgtcata
    44641 cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga ccgattttta
    44701 aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc ctggcctaga
    44761 attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag aatacggaaa
    44821 atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc caaatgcccg
    44881 ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga atgcaattgg
    44941 tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat tagaggagta
    45001 caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact atgtagaagt
    45061 ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca tttcttgccc
    45121 aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt ttcaattaac
    45181 acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc caaatgtaac
    45241 agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt ttacgatgat
    45301 caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt tagccaatca
    45361 aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga ttcaccaagt
    45421 agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag tggatgatgt
    45481 tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga atttcaccga
    45541 tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg aactaggcat
    45601 cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat gaatcaacta
    45661 gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga agactttctt
    45721 cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt gttctaccaa
    45781 gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt tttagatctg
    45841 aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc aaagcttggt
    45901 gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc ggcaatgcga
    45961 ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc tatcactcag
    46021 cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt ttctttagaa
    46081 aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg tgtggtttct
    46141 tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt ttgcttaact
    46201 ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt cacaccttct
    46261 caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac tcagtcaact
    46321 aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa aatgaatgtt
    46381 gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa tccaagtaat
    46441 ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg aatcacgatg
    46501 agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat caaagaagct
    46561 taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca tgccgcatgg
    46621 gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa agatcatggg
    46681 aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt gatcgaggat
    46741 ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag agaaggtgcg
    46801 gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa agcaaatttc
    46861 gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat tgaagcagct
    46921 ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg gaaaaaagat
    46981 cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga tttgtgtctc
    47041 cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc atcttttcat
    47101 caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa ttcatttttc
    47161 taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt atatatagat
    47221 tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga aatgttgcga
    47281 atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga ggtaactttt
    47341 atggggttga atgaatttat tttaggattg aacaatttag agacgatcac aagggcacct
    47401 ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt agcatctatt
    47461 gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg gaaactgctc
    47521 tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat caaaacgccg
    47581 gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga aaagatgacg
    47641 gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg ccgtcggttg
    47701 ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga taaggttgac
    47761 cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt ttataaggag
    47821 atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc tgttcagtat
    47881 ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa agaggcgttt
    47941 ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata tgagatgcag
    48001 ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg agacaaaggt
    48061 ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt tccattgcag
    48121 tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac gctttccttt
    48181 cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca tttagtacct
    48241 tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac agatattcat
    48301 ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga atttcatttt
    48361 gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca gctaatggta
    48421 caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga aaaacatgag
    48481 cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca ttatattggc
    48541 tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga tcatgtagga
    48601 aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc gaacaaccga
    48661 ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga gcaatgaata
    48721 ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga gaagcggcaa
    48781 agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta gaaaccgagt
    48841 tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa gaagggcaag
    48901 acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa actcactatc
    48961 taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat ttcttaggtc
    49021 ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt cggatcatgg
    49081 agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag atcggcatta
    49141 tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa ggagagctaa
    49201 gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga catccattag
    49261 ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt cgttttacac
    49321 aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct gatcaagaaa
    49381 cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa actgatgctt
    49441 acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg ttaatccctt
    49501 tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt ccgctcagtg
    49561 aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag agctaagaaa
    49621 agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt tgtgacagaa
    49681 ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg ttgtgggaaa
    49741 gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca tagctgtaat
    49801 tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa cataaccagg
    49861 cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg gcacttgggc
    49921 agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa ttgtttcatc
    49981 tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg catgaagcca
    50041 atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat tgtttttacc
    50101 aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg gtttcttacg
    50161 tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt cttctttaat
    50221 tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt caagatattg
    50281 gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt tcttcagctt
    50341 ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg gatctagtgc
    50401 tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt ttgcgccgcg
    50461 tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat tctcagcgtt
    50521 ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc gttttagttc
    50581 attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt tctctgcttt
    50641 ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta cagcagatgc
    50701 ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt ccgtttcgta
    50761 aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata caaccatctt
    50821 ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga aaccgattcc
    50881 ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc gctgaaagat
    50941 tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag caacatattc
    51001 tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt tgatcgcatc
    51061 cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc ccattaattc
    51121 aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa agtggaaatg
    51181 gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat ccaaatattt
    51241 tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga tttgggtaat
    51301 ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt tgccgcgtga
    51361 acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga tccggccaga
    51421 tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat caaaggacat
    51481 ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa gaagaaaaac
    51541 caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt acataaaaaa
    51601 cagtgtttct atcgttgaaa tccaaatttt taggt
//
LOCUS       DACECS010000016        48687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.016, whole genome shotgun sequence.
ACCESSION   DACECS010000016 DACECS010000000
VERSION     DACECS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.016"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWO31_000978"
     CDS             23..646
                     /locus_tag="IWO31_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301410.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWO31_000979"
     CDS             1096..2145
                     /locus_tag="IWO31_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0301411.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWO31_000980"
     CDS             2142..3023
                     /locus_tag="IWO31_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301412.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWO31_000981"
     CDS             3026..3796
                     /locus_tag="IWO31_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301413.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWO31_000982"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWO31_000982"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0301414.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWO31_000983"
     CDS             5107..6270
                     /locus_tag="IWO31_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0301415.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWO31_000984"
     CDS             6387..6797
                     /locus_tag="IWO31_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0301416.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWO31_000985"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWO31_000985"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0301417.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWO31_000986"
     CDS             9337..9654
                     /locus_tag="IWO31_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301418.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWO31_000987"
     CDS             9814..10050
                     /locus_tag="IWO31_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0301419.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWO31_000988"
     CDS             10047..10445
                     /locus_tag="IWO31_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0301420.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IWO31_000989"
     CDS             complement(11112..12056)
                     /locus_tag="IWO31_000989"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0301421.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IWO31_000990"
     CDS             complement(12352..13296)
                     /locus_tag="IWO31_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301422.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13523..14716
                     /locus_tag="IWO31_000991"
     CDS             13523..14716
                     /locus_tag="IWO31_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301423.1"
                     /translation="MNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAVEL
                     LTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASRAG
                     LGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQYT
                     FLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVY
                     NAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLL
                     MGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPTNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            14738..16753
                     /locus_tag="IWO31_000992"
     CDS             14738..16753
                     /locus_tag="IWO31_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAQ0301424.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16836..18209)
                     /locus_tag="IWO31_000993"
     CDS             complement(16836..18209)
                     /locus_tag="IWO31_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0301425.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18235..18519)
                     /locus_tag="IWO31_000994"
     CDS             complement(18235..18519)
                     /locus_tag="IWO31_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0301426.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18521..20536)
                     /locus_tag="IWO31_000995"
     CDS             complement(18521..20536)
                     /locus_tag="IWO31_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0301427.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20715..21020)
                     /locus_tag="IWO31_000996"
     CDS             complement(20715..21020)
                     /locus_tag="IWO31_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0301428.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21109..22140)
                     /locus_tag="IWO31_000997"
     CDS             complement(21109..22140)
                     /locus_tag="IWO31_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0301429.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22347..23222
                     /locus_tag="IWO31_000998"
     CDS             22347..23222
                     /locus_tag="IWO31_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0301430.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23374..24216)
                     /locus_tag="IWO31_000999"
     CDS             complement(23374..24216)
                     /locus_tag="IWO31_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0301431.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24308..26185)
                     /locus_tag="IWO31_001000"
     CDS             complement(24308..26185)
                     /locus_tag="IWO31_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0301432.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26182..27603)
                     /locus_tag="IWO31_001001"
     CDS             complement(26182..27603)
                     /locus_tag="IWO31_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0301433.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27606..30995)
                     /locus_tag="IWO31_001002"
     CDS             complement(27606..30995)
                     /locus_tag="IWO31_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0301434.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31252..32643
                     /locus_tag="IWO31_001003"
     CDS             31252..32643
                     /locus_tag="IWO31_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0301435.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32716..33363)
                     /locus_tag="IWO31_001004"
     CDS             complement(32716..33363)
                     /locus_tag="IWO31_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0301436.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33371..34057)
                     /locus_tag="IWO31_001005"
     CDS             complement(33371..34057)
                     /locus_tag="IWO31_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301437.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34063..35136)
                     /locus_tag="IWO31_001006"
     CDS             complement(34063..35136)
                     /locus_tag="IWO31_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0301438.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35358..36032
                     /locus_tag="IWO31_001007"
     CDS             35358..36032
                     /locus_tag="IWO31_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301439.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36037..37377
                     /locus_tag="IWO31_001008"
     CDS             36037..37377
                     /locus_tag="IWO31_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0301440.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37526..38512)
                     /locus_tag="IWO31_001009"
     CDS             complement(37526..38512)
                     /locus_tag="IWO31_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0301441.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39236..40267
                     /locus_tag="IWO31_001010"
     CDS             39236..40267
                     /locus_tag="IWO31_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0301442.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40376..41908
                     /locus_tag="IWO31_001011"
     CDS             40376..41908
                     /locus_tag="IWO31_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0301443.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42047..42859
                     /locus_tag="IWO31_001012"
     CDS             42047..42859
                     /locus_tag="IWO31_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0301444.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42849..43334
                     /locus_tag="IWO31_001013"
     CDS             42849..43334
                     /locus_tag="IWO31_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0301445.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43357..43902
                     /locus_tag="IWO31_001014"
     CDS             43357..43902
                     /locus_tag="IWO31_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0301446.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43975..45618)
                     /locus_tag="IWO31_001015"
     CDS             complement(43975..45618)
                     /locus_tag="IWO31_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0301447.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45747..47975)
                     /locus_tag="IWO31_001016"
     CDS             complement(45747..47975)
                     /locus_tag="IWO31_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0301448.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48008..48487)
                     /locus_tag="IWO31_001017"
     CDS             complement(48008..48487)
                     /locus_tag="IWO31_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301449.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14822 a         8969 c         9159 g        15737 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgattta tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcacatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc caaccaatac ttcaatctta ttggttggcg ctaatttagg
    14521 tactttcatt gcaccatatg gtttgctctt tctctctttt gcttctcttg ggagtaaaac
    14581 acttgctgtt ttcgtcaatg gcagtatctt gctgttgatt agcgcgctta ttagccttct
    14641 agtagtacac aggacgaata gaaagacagt gataagagta gatgaagaaa taaaagaaca
    14701 attgacaaca aaataaattt gatggaggtc gtgtaaaatg acaaatgaaa aacataaaga
    14761 tagatggctt tggtatcctg gagactttga gatccgccat ggactgctac aaaatttcca
    14821 acgggaagaa cgaggttttg actggccagc gtactggtac atggatgatt gccatcggaa
    14881 cgtcaagttt aagagatatt actttcttga tcaaccttcc atgtttaaag tgacgatcca
    14941 aggagttggg tatgtagaaa ttaatggaca aaaacatcca tgtggaaaat ggctgacttg
    15001 tccagctggt aaagcaaaaa ttcgtatctt tgtaggacat acatcagggc taccagcgat
    15061 gtttatcgag ggagacgaag taaaatcaga tattggctgg acagcaagta attttattga
    15121 agaatatcca gctggctgga gtccgcttta tgtcgatatc gcaaaagatc caaatcaaat
    15181 ctattatcaa aaagaatgcg tccatccagt cactgaaacg gagattggcg gaggaatctt
    15241 attcgatttt gggcgagcag tgaatggctg tgtagaacta atatctctgg atgaccatat
    15301 cgaatctatc actctatgtt atggagagtc cgatgtagaa gctatggata tcgattattg
    15361 ctactataag caaataggaa tgacaagaaa aatgaaagct cgtaaacgcg cttttcgcta
    15421 tttgtttata ccagaggttt ctactggaat ggttcaactt caagtttttc atgaattttt
    15481 atctctttcg aaacaagctg ctttttcaag cgattcttct ttactgaatc agatctggtc
    15541 agtatcagaa gaaaccttca agttatgtag tgatttgttt tttatcgacg gtatcaaacg
    15601 agatcgctgg atttggtctg gagatgcgta tcaatcattc atgatcaatc aatatctttt
    15661 ttttgacgag gaaatcaata aacgaacgat attagcatta agaggacaaa atgaaatcaa
    15721 gcagcatatg aatactatcg ttgactattc gatcttgtgg ttgattggta tcgaaaacca
    15781 ctatatgatg acgaaagact atgaattttt acgcatcgtc taccctaaga tgcaatcatt
    15841 aatgagatac ttgatggagc aaacgaatga attagggttt atttatggga gagcaaaaga
    15901 ttggatattt attgattggt ctgaaatgga taaagaagga acgcttgctg cagaacaagt
    15961 cttattactg aaagcctatc attccatgat cacttgcgga gatatcttag ctgaaacata
    16021 tggagagaac gaaaattatc atactcatct ctatcaagaa aaatatcaac tattgaaaca
    16081 aaacctattt gactactttt gggatgaaga acttggcgct tttatcgatt gctatgaatc
    16141 tgggagaaga aatgtgacgc gtcatgcgaa tattttcgct gttttattcg attttgctaa
    16201 caaagaccaa cagcaatcga tcttaaagaa tgtcttattg aatgacacta tcacacagat
    16261 tactactcca tatttcaaat tctatgaaca agatgcttta tgcaaactcg gacagacgaa
    16321 tatcgtctac caaacgattc ttgattattg gggagggatg ttatctcatg atgcggtaac
    16381 tttctgggaa gaatacgacc ctactcaaac aggaaatgaa aagtacagta tgtatggaga
    16441 tccttatgga aaaagcttat gccatgcatg gggagcaagc ccaatctatc ttttaggacg
    16501 ctattttatc ggtttgtatc caacaaagcc aggttatgaa gaatttatga tacaacctaa
    16561 tctagattca tttgaaacac tgaattgtac gctacctatc aaagatggaa agatttcgct
    16621 tgaaaaaaca aaagataaga tcgtgattac ttctgataga acaggcggaa cagtgaaaat
    16681 gaatggtaaa agttatcctc ttctagcaaa acagccactt gtcttaacaa gctattcaaa
    16741 aataaatgca taaaacaaaa aactgcttgc gacatatcag aagactttaa atcttcttct
    16801 gatattgtcc aagcaatttt tattttgaag tttgcttaat tctgttcttt agaaacaatg
    16861 tttgttcttt ttccggcaaa aagaaagcta gataatacga caagtaaaaa gaaagcgatg
    16921 attccaactg tcaaggctat ttgaccgtat tctcctagtt tcccaagtac aagtcctggt
    16981 actagataat cagtatcggc aaatgtagaa gaagcgattt taagatctcc taatactgga
    17041 aggataaata atggcagcca actaatcaat aagctattga aagcagaacc taataccgcc
    17101 cctcttctac cgccagtcga gttgccgaag acaccagaag cagctccgca gaagaaatgt
    17161 cctactaccc caggaatgat aactgtagtt tgcaagccta tcatagcaaa catggatagt
    17221 gttcctacaa agaagctaac aaaaaagcca attaatacag cattaggtgc atatggaaaa
    17281 acgataggaa catctaatgc tggtttagaa tcgggtacga gttttttagc aatcccttgg
    17341 aatgctggaa cgatttctgc caacaccatt cgaacccctt gaaggacgat aacaaaccca
    17401 gctgtgaatg ttccagcttg gatcagtgca aagattacag cgttgtctcc attgcttagt
    17461 tctctttcaa caaatccagg accagctaac aaagcaagtg cgacatatac gatcgacatc
    17521 aagagcatga tcgatacagt agaatctcgc aaaaagctga aacttttagg aatctttatg
    17581 tcctctgtag aacggtcttt gtttccaaaa aactttccga tccatccact agaagcataa
    17641 ccgatatttc cggtatgccc taacgcaact tgattgtttc cagtgatgtt cttcataaac
    17701 ggttgacaaa gagcaggcgt aatagttaat agtgttcctt cgaagacccc gcctaataag
    17761 atcatccaca aatttgcagt aaaccccgcg cttactaaaa tgactgctgt tagacaagaa
    17821 acataaagca ttgcttgacc tgtaagaaaa atgaatttat atcttgtgaa tctagcaagt
    17881 aaaatattca cgatcatccc tactagcatg atcaaagcag taggtgtacc gtattgttgc
    17941 aaagcaatcg ccactactgc ttcattgcta ggaactactc cttgagtatg cagagctgtt
    18001 tggaacatcg aagcaaaagg ggttagtgaa ttactgatta aaccagcacc gccgatcagt
    18061 actaggaatc cgacaaatgt tttgatagtt ccttggatca cttctgaaaa tttcttcttc
    18121 tgaagaacga gaccaagcag tgcaatcaaa gaaacgagga tggagggctg actgacgatt
    18181 gacacaaaca aattcaacaa tttatccatt gatagttcct cccttgaatc atttttattc
    18241 ttttgacaag atcttcgtta ctttttcttt tacttcttcg ctgtcaatga tcgattgcaa
    18301 aggtacgagc ttttcatcag gtatagaagt tagcgtatct ttcaaagaat gctcaatgaa
    18361 gaaataatca gccatatcgc tagatacact tccaacatcc gtatgactta cttcaaactc
    18421 gtccacatct gctccaagat ctgcaagtgc ttgtttgatg ttcatctcta ccataaaact
    18481 tgttcctaat ccactttgac atactgcaaa aattttcata tcattcgtct ccttttctta
    18541 tgatcgctaa tatttcttct tttgttgatg cgtgtaataa atcttgaaga ttttgtttat
    18601 tagaaagtag ctttgttaaa tttgccaaag cccgaagatg tgcctcatta tcgatagcag
    18661 ctaaacagat gaacaaacta atgggatggg ctggattatc aagcagatcc acaggttgtt
    18721 gaagtttcag caaagacatc cctacttcgt ttacgccatc ttctggacga gcatgaggta
    18781 atgcgatgtg atcgccaata tggataaacg caccatactg ctccacgcgc tcgatcatag
    18841 ctttgatata actttcttct attttatttt ctgataatag cggctgtgcg gcaaatgtga
    18901 ttgcttcttg ccagtttttt ggttgatccg ataattggat catttcttct gttaataatt
    18961 cagatagcat aggacgttga tcctccttct tttctaaaag tttgttcatt ttacgattta
    19021 atacttgata gagcttttct tcgtctacgc cttcttttaa ttcgatataa ggcagtagcg
    19081 ccgacaacaa ttcttgggca gaaggcattg aaaatcctgg tatcagccaa tctctttgta
    19141 cttgattgat cagctgattt ttttccaagt cagtcatgat tggatgcaga acatagagag
    19201 actttttctt atcagtaagt gtgagaggta cagtagaaaa aataatatcg taagaagttt
    19261 cgtcaacctc gtgtaagcgg tcagctgaat tagtctctgt aaaaaagatc atcgggaata
    19321 attgctgaag ttcacttttt aagatcaatg aagagctaat gccacttgga cagacgatca
    19381 cggctcttat ttttctattt ctttcttctg cgtccgcttt tctgatctct ccgccaaata
    19441 gtatagtgaa aaaaccaatc tcttcttcag ggatgctctt ccctattcgt ctttctaacg
    19501 gaagtaatgc ttttcttgtc atttcgtaga tggaagcata ttgatgctta atttgatcga
    19561 tcatcacatt aggtaaataa aaagaatagg aaatacgaaa ataggcaggg accaagtgat
    19621 aaaagacatt catcaataat tcacgaaacg aagcaaattc tacggccatc aggaattcca
    19681 tgcgatggat gacttctgaa gaacattcca gcaaaaaatc aagtgcaggc tcctggacat
    19741 ctccttcaac gatcgatgct aagcaaagag agaaaaatat aatctccgaa ttttgatgtt
    19801 cggaatagcc aaggccagtc aatacttgtc gactaatgtc agtgactgca agagaagcca
    19861 acagttcagc atcttttacc agttccatgg atgtttttcc aattcgaatg gttgtcaaac
    19921 tcaaataata agctaattca tccattcgat ttggtgcgaa tcgaagatct gatagtgaaa
    19981 tcgttttatc tagcaaatct ctaactttgg cgtaataatc aatggaaagg gttgcggtta
    20041 aatacgtgag cccaaatctt ccagcaagtg actgcatcaa acgagcagtg cgattttttg
    20101 cttctcttct taatattgct tctgacccta gcagcctaaa accagttttc ctagtatacg
    20161 acagctgaca atcttccact tctaattcat ttcttatctt tttgatatct gacaggactg
    20221 tccctttaga aacctgaaga aaatcttgaa aatgaaaaac agaaacagac gttgcagtcg
    20281 aataaaccat caaataaaga agatctcttc gctcttgttc ggtatacaca gcttcaaatc
    20341 gagtaatatc caaaaatttt tctgcccagt cgattgctcg gaatttcttt atacggatac
    20401 gggtatcatc cacatattcg gcaaaaggag ttaattgttt gatcgcctcg cttgtattta
    20461 cttcgatgat ctctgctgct ttttctagac tgatttctct tggtgttttc aggagataac
    20521 taattttgta gttcacagcg ctgttcacct cctacttata ttctagtatg gtagcgcttt
    20581 taaatcgagg ggtgtttggt acaacttaca gttggatttt ttttacgact gtaccgaacg
    20641 atttctatcg aatggataca gaaaaaaagc aggtgaccaa atgtctcctg cttttatgaa
    20701 ttgatttcgt ccgatcaatg atgatcatgt ttattttttg aggtcagttc ttcaaatttt
    20761 tctttgacat cttcaaccgc tttttcgata tctttttcaa agtgattggg ctcattaggc
    20821 tttgtttcct cttctacaga atttgaacca gataaagctg tttctttatt ttctgtttca
    20881 ccagagtttg tgccagctaa tgattcttct ttgtcttctg tttcaccaga attagttcct
    20941 gctaaagcca cttcttcttc tgttttaggg gccattgaac ctcttaatcg tttgtgtttt
    21001 tcaccgtcat aatttgtcat gttggttcct cctagtagtt ctatactttt tcatcgtaca
    21061 ataagacagt ttatgcgtca aaaaacaaac ccgattctta caaatatgtc atatttttcc
    21121 ttctgattct tgaatgcttt tgattccttc ttccaaaatt ttcatttgta agattgtttc
    21181 ttcatctttg atgacttttt ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac
    21241 acggctgtaa tcaccaacct cggaaattac tttttcttca tgataactcc cttgttcatc
    21301 tatataggtc aatacaccat aatgttcggg caaatcgata ccaaagtctt catgatcagg
    21361 catgtaaaac attttcagat gttcttcttg tcgatctttt gtttgtttca cgaacatccc
    21421 tttttttccg taaacgacga aacttggacg ttctttgatg cggaaataac tggattttat
    21481 cgaaattttc atatttccat agaaaaaatc cagatcgaag taatcattca ttcgtccttt
    21541 ccctaaaagt tgacgtacat catagctgat atcatctggt tcgccgaaat aagaaagaac
    21601 ttgatctact gtatgacaag catgtccata taaatagctg ttcgcagtag aaaattcatg
    21661 cgtattttct ggtacttccg gtctgaaata atcataatgc atttctactt ctaaaatatc
    21721 ccctaatttt ccgctttcga ttactttttg gactgtcaag aagtcagaat cgaaccgtct
    21781 gttttggtaa cattggataa atagaccttt ttcttttgct aatgcaaata actcttctgc
    21841 ttcctttgac gtttctgcaa aaggtttctc tactagtaca ttcttaccat gttcgagtgc
    21901 catttttcca tattcataat gaaattgact aggtgtagtg atgacaacta attggatctc
    21961 aggatcatcc caaagatcag acagctgatc agtatattcg atgtcttcga atgttttcca
    22021 cactggttca gatggggaaa aaattttttt gatattgatt ttgttcgtaa gttttcttga
    22081 aaaaggcaaa tgatagcgat ttgtactctt tccattgcca agataagcga ttgtaagcat
    22141 gagtggtgcc tccttttatt gatgttttca gtatatccta gaaaagcgca gaaagtattt
    22201 ataatagcta ttctggtaaa aaagttcaga aaaacacttg ttatcattcg gtttggtgaa
    22261 attaatgttc aaagtaagga taaaaatcaa aaagatagta gcggaaccaa gtgtttatca
    22321 gtaagataga atttggaggt cagatcatgc ttattgaaga aaaactaaca aaacaagagt
    22381 tgtttaccac gacagagaaa cggattgtcg attacatccg taggaatatc gaagctgcag
    22441 tctacatgac gattgaagaa ttagcaaaag ctacgtatac ttcccattca gctatcattc
    22501 gtttatgcaa aaaaatggga ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg
    22561 aagttcatca attgattgct cagttcaatc aaacgattga tcctaatttt cctttttcaa
    22621 gtgaagatag cactcagcaa attgcaaaga aaatggcgga tttatcgatc cagtcaataa
    22681 aaaaagcaca attgcagatt gaacatgaag atttagatca aatcggtaaa gtattgaaca
    22741 aggcacagcg aatctttctt tttgcaaaag gtgattctca aatcacggca aggaaattcc
    22801 agaataaaat ggtcaagttg aataagtttt tgatcatggc tgaagaatac agtgattctt
    22861 cttggaatgc agccaatttg actagcgagg attgcgccat ttttattagc tacagcggtc
    22921 gcatccatca ttatgaacga ataatgacct atttgaagca tgtagaggcc cctaccttgc
    22981 tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat
    23041 cgcaggagga atatgatttt gctaaagtag cgactttctc ctctcaaatt gccttcgatt
    23101 acgtattaaa tacgttattc tcagtgatat attctcaaaa ttttgaagag aatatccgca
    23161 acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct taaagaatat cggaaaactt
    23221 aagaaaagat cctcatttag taataagaaa gatagtgttt gataggctct ctttgatatc
    23281 atcataaaaa caggctgaag ttttcttttt caaacctcag cctgtttgtt tacggaacga
    23341 ttcgaataga tatattatag aaatgaggaa tagctaactt ctgtttctta ttttgaacga
    23401 ttgacgaaaa agatatataa gaaggaggaa aatcagtata catgcagcta acaatccata
    23461 caggaaaaat cgtttgttct cggatacttt ctgtacttct ttttgaactt cttttgcatg
    23521 ataaggaacc cggtgccctc tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca
    23581 agttaggagt gtcacgaaat cctgcccttt ttcgattctc agatcttttg tatcagtcgg
    23641 ttctaccgtc ttgatctgat cgacttgata agcgagtgtc cgtccattga tttcgatata
    23701 gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc
    23761 tcgatggctg gaaataacgg catgtgtatt ttctcccccc gtaggataag acgttccttc
    23821 caataatgag gcaccttttt ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg
    23881 gacattgatc tcagggatcg tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc
    23941 ctttttcgca ggttcttttt tcttagtaaa gggatctgca cctggatttc caccttcttt
    24001 tgctaattgc tggtttttct ttgtcatgtt ttcttggatt ttagccatga cttcttcatt
    24061 ttctttgacc gcttgttgct gataatgaga gatgatctgc tgatccagat aattattcag
    24121 cgcgtcgcta acaaatggat aagctagtat ccctattcct gagagaagca agaaaagcat
    24181 cagcagatct agaagtttcc ttttctttgg tttcatttgt gttctccttg tgatgactgt
    24241 ttgataataa aagaaaaagg agcatttgcg taatccgctg ctcctttttc caattcttgc
    24301 aaaaagacta aatctgactg tgcttgcgtc tagcaaagta cagtccagca atcagtagaa
    24361 ggactactcc tgcaccgata tacacataga ttcccttacc gcctgttgaa ggaagtgtac
    24421 ctttgtgttt atttggtatt ttttcaggag aaatcaactg tcctgctgtt acataagatt
    24481 gttcatcgat agtaaatgct acacggtttg ttaatggaac atatttttct ggcgctttcg
    24541 tttcttccag atagtacgtg ccatatttta gacctgtcac atcgattaaa ccgttacttg
    24601 tggttgtaaa aaccgttgct gattctttcg ccgataccca gctgacggct tttgtggatg
    24661 ggtcgatcga taaatatttc gctgtgtcac tatcttgatc acgaacgacg aattctgctc
    24721 cagcaagtgt ttggtctgat gtaacgtcac catctacttt aacaaatcgt ttgcccccag
    24781 taacgacatc gactgacggt ggtgtttgat catttgtatg cccgttatcg acatttgctt
    24841 ggttagaaaa tcctttggtt ggatctgctt tttcgttcaa atgcatatag taaacgaatt
    24901 tcaatgtacc gccaggagtt aatgaaggaa tataattcgg atcaactgaa accgtgaatc
    24961 cgtctgtttg ctcagtgaca gaataattta ctgggtcgat ttctttattt ccatcatata
    25021 aggcataagc atacgttcct gaagaatcat tatcaaatgt taaagcagcg tcatgagtat
    25081 cgataagttt gaatgttgtg tacttgtttt gcgttccttc tttatcagcg atacctaatg
    25141 ggatattgac agaaatctca tattgaattt tttcaccgat tgcgacatct tttccattca
    25201 attgaggtgt tgttttatca actttagacg tatcattttt gatggtcttt tctactggtg
    25261 tttggctatt tgccaggatc tcaaaaggcg tgattgtttg cttttcaatc atttccgcat
    25321 tatcaggagc ttttacttct tctaaattat attttccaac ttctaaatga ttaacgatca
    25381 actggccttt ttcaatagat gcctcggcaa agtcattgtt gccgatgtca taagaatgac
    25441 cagtaatgaa atgtttggct ttggtttgat cagttgtcca agtgtacaat ccatctgtga
    25501 cactttggat gtattttttg acgcttggtg ttccttcttc tttagaaata ataaattctg
    25561 ctccatttag tgcttcgttt tcggcagtac cgatttttgt aactttcaac gttccatcat
    25621 tacagactgt atttttaggg tagagatgga tagtatctag ttcttctgtc ccgtatttat
    25681 aagagccatc tgcttgtttg atcatctcat atacagggaa agctaaaacc atgtttgcgg
    25741 cagctgacac tccgtctttt ggttcttctt tgatcgtgta gactgcatct ttcccatttt
    25801 gttttttagg taaagataaa gtgacatttc catcagcatc tgtcgttcct gaagcaactg
    25861 gtgtaccagg agtcaaagac tggactgctt gttttgctgc atccacggac gctcctttat
    25921 ctcgttgcgc ataaaattct tgagtgacgt tataaactga aaatgaaata tcggctaatc
    25981 cttggtattg atcgaattcg ctcatttctt tcccgctgtt ttggattaaa ggatcgggta
    26041 aatcagtcat tttcttttta tgaaggatga cttgagctgc tgtctcctct gcaaacactt
    26101 tttttgcgcc gaagctgttt gtgagtaatg gtaaaataag gaaaaggact cctaacataa
    26161 cgtttatttt tttatgattt ttcatttttt cactcctgtt ctttttttga taaacaacca
    26221 gattgcaaag cttgcgaata ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt
    26281 tgtttttgga agaaatggac ggctattttc ttttttattg actactttca atatactgtc
    26341 cgcctcttta tcctcagaaa gaaaagttga aatggtaaac ggaatttctg tttcacttaa
    26401 aatataacca gaaggtgctt cgatctcttt taatcgataa cttcctgttt tcaacccgga
    26461 aattgaaaaa gcgccatttt tatcggaaat caggtgaagc gcatctgatt cgttctttgt
    26521 ccacttgtat ccgttggctg tttgctggag gtaatttcct gctttgtttt taataaggaa
    26581 tactgcgcca gctaatcgtt tatctggttt atttgtctcg attttgacaa attgcttact
    26641 ccctgtatat acagagactt tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt
    26701 gttgttgatt tccttgttcg cctgtgctgt actgcttagc tgcatctgat aagaaatggt
    26761 cagttttttt ccagcatatt tttccaacga agggatcgaa aataaaacat caaatccgtg
    26821 aggtttcttg tgtatgacaa agccttctgt cagcttttct ccattcgatg aaatcgttaa
    26881 actattaggt aaaaacgtta atgcttcatc cgcactatct gacaattcga acttttgata
    26941 gtcaaggata tttacaggaa ttgtcgtatg caaggaataa gtgatttttt caccaatcgt
    27001 aaaatcattt ctaggctctt ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc
    27061 ttcattttta ggatacaaat ggatagttga aagtttttga ttgttttgtc catgtacagg
    27121 aagaacaact accatattct ctgccttttc tttgacgact tctggtactt tggattcaat
    27181 gaaaagatac gctttatctc ttttttcttg atctttagcg gccagagaaa aagaagcgat
    27241 accatcttcg ttgttaagag ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc
    27301 accgtttttt gcgatctctg cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg
    27361 gtaaaaagaa tctgtgacat cataaacttg gaatgtgaca ccatttaatc ctcgatacgt
    27421 ttgtaataaa gctttttctt gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg
    27481 gaaaagcaat ttgtgtaaag taatctgaac catttctgtt tctgtttctg ttgctacctg
    27541 agtaaaagct ggtttaaata gtaacaagaa gagacacata ctaagccaac caagtttttt
    27601 catatttagc tgccccctct ttcttttttc aaaaaaagat accaaataga aaacgcacaa
    27661 ccaatcatcc ctactagata gattcctaaa cggccaattc ctcccgtttc aggtaatggt
    27721 acttttgcct gattcgtgat gtctaaagaa atctggttgt ttttggctcc tggtgacaga
    27781 acagattcat gatcttgtcc atccacttga attgacccat cttcgtgtat aactatagta
    27841 actggctctt ttagaccttg gtatccttct ggtgtaaaag tttcggtcag cgtataagtt
    27901 ccaggtttta aattttcgaa tagaaaggta tctgtttctt gtccatcttt tggcgattct
    27961 aagtccattt ctggtccctg cagtctgaat tttgctcctt ttaatgtctg tccgttgtct
    28021 tcttttttgt gtaccgttag atcaaacgct tttagtcgat tttgatgagt gagcgtccat
    28081 acattatttg tcatctcggt attttttccc gtgtaaatga tttttccata tttatcgatt
    28141 tggaattcat aatttcccgg cgaagtatca tatccggcag gaccagctgt ttcaacgact
    28201 cgatatttac caggtgtgag tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg
    28261 ctgtcaattg gctgataagt accatttgct tcttttttct gtagggaaaa tgctgctcct
    28321 ttaagattta tctctttgtc cgtcccttgc atcgcatact tacgtactgc tacaggaact
    28381 tcaacaaaag gattttcaat agtcaactgg atcgtatcat cggaagtagt gactgttttt
    28441 ccatcaatgg ttaccgtacc atcagaagcg atcttgattt cccaagtagt ctttttgctt
    28501 aatccatgcc cttctggagc ttttgtttct gtcagcgtat aggtcatttc tttttgcaat
    28561 ttgacatttt ctggaagaga ataggttccg tcgccatgat ctgttagtaa agtatcaata
    28621 gaatctcctg ttaatttgaa aacagcgcca ataagatcct tttcacctgt agaggaattt
    28681 ttcgttattt tcaagtttaa cggtgtgaat tgtttagtat ttttccatgt cattgcatct
    28741 atttgttgag aatcgtatcc tggtacaggt aattctttga ttgtttggta actgaatgct
    28801 tgaccttgat tattgtattg aggtagtgat aatatctctt gataactttc tccgctattt
    28861 gcagataatt tgtcaatgtc tgcacgttcc cacgtatttg ttgtatcttt agctggttta
    28921 atgattcgaa tatatccgtt tttccaagct gcagcatttg tcgtatgttc ccgttgaatc
    28981 tcaaaagtaa cttgatctgg acgatcagct agattgttgt caaattcttc ccataacttt
    29041 ttgatgtgaa gactgactcc gggagcttta gcagatggta taccgaattc agctaattca
    29101 ttggtatcaa tgtttggctg gaaagttgtc ctgccgttca tttgatacca gaaatttgga
    29161 tggaagtcct cattttctgt ttggattctc acttggtaat ggatttggat ttcttggtct
    29221 tttcctaaat agatctgatt gctcttgatg gtatttcctt ctatggaaat agatggagtg
    29281 accgttgtag gacttgtccc cacactcttg actgaaagtg taccaggctg ataaatgaat
    29341 ggttctgcga ttggatcatt tatttgtcca ttgcttacag ttgctgagat ctgtacagct
    29401 tttttggcta ggtattcaga gatatctgtt gcatgatcag ctgattcata gtaaagatcg
    29461 cctttttcat ctgatgaaac catttgtcgc atacgagact ctacttctgc ttttgagaga
    29521 ccagctgccg gatcgctttg aagttggatg ccaagaccat gtatttcgat tcctcgttct
    29581 ttgagtgcca tcgcttctcc gatggttgcg ataaacgtac tgtcgattct tctggataga
    29641 ttgttttggt caggtgcatc atagattctt gagattagag aagtatttcc cggccgatct
    29701 tgcgtattag aaaactgagt tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt
    29761 actttatagg aaaatgttgg tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca
    29821 tttggaacgg atagcatgct tcctgcatct cttagtgctt tttgtgtaaa agtaccacca
    29881 tttgtccgtg aaggtgtaat ggattttact tgatttttca ctgaatcaaa tgaacccatc
    29941 tgtactgcac cgttactgta gctatatcct tcgcttgagt agccgacata tcccatattg
    30001 attttgtctg tgataccgct atctgctaaa gtatcgacaa aacgatcgac accaatcttt
    30061 acttcaccga tccgattatt gtcgttcata cttcctgacc agtctacgac caatacgaga
    30121 tcaagaggcg tgatatcttt ttgtacattt ccacgagcat tcaaataaac atcaaacaat
    30181 ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat agtctgcttg attaccggtt
    30241 cctccatatt cgatatacga atgggtccga tcattaggat ctccattcca agaatttata
    30301 ccatcccatt gatttgttcc gtttttattg ccttgatgat caagaacatt tgtatttcct
    30361 gtaggttgcc aactggcttt aggataagtg ccctgatcat ctgttgtata ttctgtagga
    30421 atcgtttgat tagatggcac actcacagct ggcaaggata atgtaggcaa tgtaaatggg
    30481 ctgacagaat tttcgttttc ttttgatgat tcttcttcta tttcgtctgg ctttggtact
    30541 gacacatctg tatctttctt attttctgtc tcatcaataa tgtcatattc tttattatcg
    30601 acgatcagct tgtttgcctg gctgtggtaa agtgctggat cgattggaaa actcagatcg
    30661 atagtttgtt tagtgtttga tgagatttcc aacgtaattt tcccgctatt tacctgataa
    30721 gcatagccat ctccttcttt tctttccaca tcacttaagt aattttcgac tagaccaatt
    30781 tctttttcta tcttttcagc actggtgtta ttcgcattta gcttgatgtt cagtgctttt
    30841 tttccagact gatccgaaat gacttcagct gaaattgttt ctaaaactgt tggatcatct
    30901 gtagtgtctg ctattgcaga taatggcaca aagtttgata aactcaatat tatcagcatg
    30961 aaaataactt tcagtttctt ccctgtggtc gtcatttttc ccctcctttt tctttttttc
    31021 tgttttgacg tgaatagtga aaattattaa aataaacagg cgttacataa ttttatttat
    31081 tttaatatga taattattat acgaagggcg ctaaagggaa tcacaccaat tttcatacca
    31141 attggtttca tttatgaagt tgattccttc gattaatgag cgtttcattg aaagaaaagg
    31201 gttgagggag aaaaacatat gatcaggttg ttggaatgca actatagaaa gatggaaaaa
    31261 atcattcgaa ttctgtccat ttctgacaag tggatgacta aaaaagaact tgccaaacga
    31321 attggtagtt cagaatctac atttgtaagg tatatcgaag agatcaaaat ccgctgggga
    31381 acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta
    31441 ccagcttatt taaatgttct gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta
    31501 aatgagatta ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa aatttccatc
    31561 agccggtctt cttttacacg taagttgaaa caatgcaatc aaattttaga aagctattta
    31621 ttaaaagtga tcgttgacca aggttttcag ttaactagcc ttaaaagtga gctccagttg
    31681 cgtatctttg ttaccttttt ctttttaagt tactacggaa gacgcacgtt gccatttcca
    31741 atcgaaaaaa agaagatcaa agaaattatg gtcaagaata attgtcagat gaatttgttt
    31801 caggaagaaa gcccatatga gcaatctttc tttattatgt attttgttgt gcatctcatg
    31861 agagaatccc aaggaactcc atttgaatgg aaagctggag actcttatgt acagttgata
    31921 aatatcgata aaaaagactt cttaatcttg aaaaatctct taggaaaatt gaagtacagc
    31981 agctatataa aagtagtgaa ctattttgtc aatacgattt tttcagaatt ttcggagaaa
    32041 gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt cctactgttc gttttcatcc
    32101 gctgattgca aagaaaaaaa cgaagaatat gttgttcaat tattaacgca aatttatttt
    32161 ttctcccgta ttttcccata cgatacgact catttgacga tccgtgcaac ttactttgca
    32221 cagcagatgc aacaccgcca gatacgtcta tatgctggat ttcgtgcaga acttcaattt
    32281 tattccactc tactgaatca gaatctgatt tattatttat ctagctatat cttttggatg
    32341 cttatcggta atcctgaaat tcaccattat acttctgata aaaaaatact gattatcagc
    32401 gacttaagtc ttcgacatag tcaatatatt gaagaatata tcagtgatat tcttgctgtg
    32461 cataaaattc actctgagac aactgctatt actgaagatc agctgtcgaa atacaatctt
    32521 ctcgagtatg atctgatcgt tacgaatcaa cccattttga atgcccatgt tcctcatata
    32581 ttgattgatg atagtgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta
    32641 tgaataatca aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca
    32701 catagctgtt tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc
    32761 ttttaacaac tcttcttttg tccaattatt tgcaatgaga tccgtcaaag tgttcattac
    32821 gagggtcatg gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg
    32881 attgattctt aataattcca gccatttttc gttcattgga gaatcagccg atagttctct
    32941 acgtttggtt gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat
    33001 actttcgtga tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg
    33061 ttgtttgttt tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt
    33121 gatcgtatac aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga
    33181 catccccatc ttgtcaacga tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa
    33241 tatctccagt aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt
    33301 ttcttttggc atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt
    33361 catgaaggat tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat
    33421 aaacgcgatc actccattga atcatacgga catcatgagt aaccataata gtcgcttttt
    33481 gtttctcatg agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca
    33541 aactagctgt tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa
    33601 ttgccactct ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact
    33661 ggatatctaa agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa
    33721 cttggtcaac tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt
    33781 ggagaataaa tcctatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag
    33841 ttagtgagtg accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa
    33901 tggtaaggaa agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt
    33961 cctttacata aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg
    34021 ttttggtaac ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt
    34081 aattgggtct actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat
    34141 gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct
    34201 tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc
    34261 aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc
    34321 tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac
    34381 cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc
    34441 agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga
    34501 tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc
    34561 ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc
    34621 atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg
    34681 atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt
    34741 ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga gttttggtaa
    34801 taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat
    34861 tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc
    34921 tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa caatctcttg
    34981 tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga
    35041 caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa
    35101 tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat
    35161 tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa tttgtcactt
    35221 gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg
    35281 agtcataaaa aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa
    35341 atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca
    35401 ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg
    35461 aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc
    35521 cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag
    35581 tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg
    35641 cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat
    35701 tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt
    35761 gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag
    35821 agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga
    35881 tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata
    35941 tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac
    36001 gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag
    36061 caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg
    36121 ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta
    36181 gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc
    36241 gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta
    36301 atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag
    36361 atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat
    36421 acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc
    36481 ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc
    36541 caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg
    36601 attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt
    36661 ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct
    36721 tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc
    36781 aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg
    36841 aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag
    36901 cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca
    36961 tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta
    37021 gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca
    37081 ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa
    37141 tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt
    37201 tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt
    37261 ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat
    37321 tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc
    37381 ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa
    37441 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt
    37501 ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat
    37561 caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact ttttcaacat
    37621 cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat
    37681 ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc
    37741 cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg
    37801 gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg
    37861 tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta
    37921 gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag
    37981 gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc
    38041 ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca
    38101 ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct
    38161 gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat
    38221 ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat
    38281 cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt
    38341 caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat
    38401 aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg
    38461 cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc
    38521 ctcctagcaa atagatttat gattggtata ttgttactta taaatttatt ctataggaga
    38581 cgattgtttt ctacaaatat ttagattgga atttgattaa aacaatttaa ttgaaatttg
    38641 aaaacattta tgttaatgaa agtcatattc taataaaatg tcatttgttt ctatatgaat
    38701 ttttatgaaa aatttcatat aatttccact cgttgtgatg gtttaattta tccaatccat
    38761 caataagcaa acaacttata taaaaaagga ttaattctca atataattgc tcctaccata
    38821 gaataaatct tcatgtaaag taatgaagta ccaactattt ctaatacttc tttaagaatt
    38881 atcccaacaa tatcgttaga caaaccatta ttgaataaat ggagagccaa tcgattctga
    38941 aaaaatgttt tctaattttt cggaaataaa agaaagagcc tcctgccatt agaacgaacc
    39001 aaatccattt cattgctcat gaagtaacca catagctaaa aagaggataa atcaattaaa
    39061 agatcaaaag aatgactgca ctaataaaat aactaaaaat gtatcttctt acttcatatc
    39121 ttttagctag tacttagtta aaaatttgct tcctaagatc aagacaaact gtctatcttc
    39181 atttgatgaa atgcggtaaa atatagaaaa tgaacactat tggagggaat ttgtaatgga
    39241 cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa cgtacacact tgctcccttc
    39301 cgtctttcaa cagtttttag aagaaaatcg ttcttccctt tcattaggca gtcgctatga
    39361 atacgcaaaa gattttcatc tgtttactga ttatttgaga aaagtatttt ctgaagaagt
    39421 ggatgatcag ctgattatcc atttagaaaa acaagattat cagaatttct tagctaaaat
    39481 ctatcgacat actcgttcgt tccaaactac agcgaaccaa gaaacggagc aattgtttga
    39541 aaatagctat tacgggattt caaggaagca atattcatta aatcattttt tacgattttt
    39601 gtatcaaaaa ggactgattg aagaagagat ttctcttttg ggtacaagtt taccggaaac
    39661 aaccaaagca gctccgcgat atatcgatgc agaacttttc aaagattttg agcatttgtt
    39721 tatcccgatt gaaggctttc aaacaagcag agaagctagc tttgaagaaa agaatagacc
    39781 gaggaacctt gcaattactg ccattcttct gtattgcgga ttaaagatcc atgaagtagt
    39841 agaacttaaa cgtaaagatg tatctttgac caagcagatt cttaatctga atagaaaaga
    39901 aaacagactg aataaagtac caataccaaa agaagcgatg ccatttctgg agaactattt
    39961 taagttaaca gcacaaaata cggacgaaaa ttcttttgtt tttcgttcct cacatgatca
    40021 gcaaatttct cctcggacta ttcgccaaat tttgagcaaa attacagtat ataaaaacgt
    40081 atcgccagaa ctatgcagaa aaacatatcg ttattatgca gaactatatt tagatgatgc
    40141 tgacgcggta aattatttat gtggtaatcg atacctctca tcccattctt ttcaagatat
    40201 ctgggaaaag atggttgatt tttcttatca cgaattagcg gcattcgttc gggtttcttc
    40261 catataaaac aaatgtatga tctttttttc acaataaact atgttattat ataaaggatt
    40321 atgttacgat tatcttgaat ttgtatatta cttaacaaaa gataatggag tgtgaatgat
    40381 ggagaccata acgtattccc ctttgaactt atttacgaat tttcaagaag ctgcagctaa
    40441 cttttccgag accccgatta tctttgatga gccactttca gctttcccag aattaggact
    40501 tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa cgggcttatc aattggctca
    40561 tcttggagtg aaatccggag acaaagttat tatttataag agttccgctt ttgacacata
    40621 ccttctagct gtttctgtgt cttaccttgg tgcagtgcct gtcatgactt cttatcatct
    40681 accaacaaca acaatggaag tatttatcga tcgtttagaa gaccctttca ttttatttga
    40741 tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga acaaaatcaa aacagattcc
    40801 tattctttat ttaatggaac aacctgcttt gtctgtttct caatcatttt tagacaaaaa
    40861 tgaaatttct tatatgaccc atacttctgg tacaactggt atccctaaac ttatttgtca
    40921 ttctgctcat tctatgggat ggcggacgaa atggcaaaaa acagttttca caaaaatttc
    40981 ggaaaagaaa ttaatcgctt ttcacatctc tccagtgcat tccagattca atattggtat
    41041 ctcttctttg atgtccatgg gatttcctat gatgccttta gcaaacgcac aaagttcaaa
    41101 agttgttcat atgttggaag ctcaccggcc aattgcacta gagacccatc cgaataactt
    41161 tgttcagtgg cgttttactg ctaaggagca tccagaagct ttttcgggta tccgatacta
    41221 tcactccact tttgatgcta taaacaacca gacaatgaag acatttttgc gaacgtcgat
    41281 ggcacaggat gcggtatttc ttcaagtata tggccaaagt gaatgcggac caatgattct
    41341 taaggctcat acactagaga gtttgaaaac aagtgatgca agagatatgg gcgttggact
    41401 ggaagacatg acaagcgctc ggattacaga ttcagttggg aatgtcctac ctgaaaacac
    41461 agatggtcat atacaattac tgtctaaagg acgggctttg acttattaca aggaagatgc
    41521 acggttccag gaaaatgttt atggcgattg gtgggatagc ggagattacg gttttatgga
    41581 taagcatggc cacctatttt tgaaagatcg gcaagtggat ttgatcgaaa cgatcaatag
    41641 tacattagct attgaagact ttttattaga ttcgttagat tttttggcag aagtagtgat
    41701 tgtaagggat aaaaatcatt ctccacaacc gattattgct ttagctcctg ataaagaaat
    41761 ggattggaat cgatggtggg agcaagtcca tgaacttcct cgcttgaacg tccctatcat
    41821 ccgtgatttt gatgcaatcc cgcgaacagc tacaatgaaa gtgcaacggc ttcaaatcga
    41881 gaaagaatta aaatcacaaa agtcctaact atttaaggaa agaaagatcc attttggtct
    41941 ttcttttctt tttgtttttt taaattggat gagataaaaa tatgccaatt ggctgttttt
    42001 cttttttgaa aatttcgtta tactcttatt catggaggaa aatgttatga aaagaatctt
    42061 gacaattggc ggttctgatc catttgctgg tggaggaatc caaacagatt taaaaacttt
    42121 tgagaatttt ggtctattcg gactaagcgc cttgacttct ataggggcac tagatattaa
    42181 tgagcgattt atgctggaat ctatatcttt ggaattgctt aaacgacagt tagtttcgat
    42241 tgataaaatg acttcattag atggtatcaa aattgggtta ttgaattcaa gtgaagcaat
    42301 catggttgtt cgagacttca taaaaacaaa acaaaatatc ccaatcattt tagaccctgt
    42361 tttggcattc aaagaaactg aagctgtgat caatcaattg tatatttcgc agctcatcga
    42421 agaacttttt ccacttgtta cgatgattac gcctaatttg gcagaagctg ctttactttc
    42481 tggtcaagct cttccctctt cgttaacaga aatgatccat ctcgctaaag aactcactgc
    42541 ttcaggaaca aaaaacattg tgatcaaagg cggcacaagg atggctggag atgaagctgt
    42601 ggatcttctc tattcaattg atcattatga ggtattttct cataaaaaaa tcgacactac
    42661 aacagtaaac ggggccggtt gtgccttttc ttctgcaata acagcgaatc ttgtgttagg
    42721 ggcagatttt accaaagcag tagcagaaag taaaaaattt gttgcccatt gtatttctaa
    42781 tggcgtgctg atgaacgatc ataccggaag tgtctggtct ggcggtattc aaaaaggagg
    42841 aatgagaaat gaaaactaag caattaacga tctatgctat gctgattgcg ttgacagttg
    42901 ccctttcatt aacaattttg atccctgtac cggctacgaa tggatttgtg acattatgcg
    42961 aggccgggat ctataccgct gcttttcttt atggtccaac gggtggatta gttgttggtg
    43021 cttccagtgg tctattgatc gatttgattt caggatatcc gcagtgggca gttttttcat
    43081 tagtgatcca cgggttacaa ggatatgttg ctggttcttt cagcaaatct tctactcgta
    43141 tgtggggatt agggctttta ttaggaagct tagttatgat catcggctat ggtatcgccg
    43201 gttggatttt atatgattgg ccggctggag tggcttctat accaggaaac atcttccaaa
    43261 atctgattgg aatgggactt gcctttccta taactgcaag tcttcaacgt attcaagcac
    43321 ggaagcaata ttaattgaaa aagaaggttt tagaaaatga agttgtcaga aaaagaatta
    43381 aaagaccaat tgactgaaat agttaatgac atattggctg aggcacactt gaaaaaaggt
    43441 gatctttttg ttttaggatg cagcacaagc gaagtcgttg gaggacatat tggaaaaaat
    43501 tccagtgcgg aagttggtca atggatcatc cgtacgttaa aagagttgtt ggatcctaaa
    43561 gaaattgcgc tagctgtcca aggttgtgaa catttgaacc gagcattagt agtagaacga
    43621 acagtggcag aagcaaaaaa tttcgaaatt gtctctgttg ttcctgcact acatgctgga
    43681 ggtgcttgtt ctgttgctgc ttttgaccag ttcaatgatc ctgtcgaagt ggaacacgta
    43741 gttggacaag ccggtatcga tatcggtgac acatcaatcg gtatgcatat caagcatgtc
    43801 caagtacctg tcagacctcg tttgaaaacc cttggacaag ctcacgtgac agcccttcgc
    43861 tctcgtccta aatatatcgg tggtcctaga gcgaattatt gaaatgagta attgacagag
    43921 caatctgaaa aacttcctcg caaagtccaa ctaatgaact ttaacgagga agttttactt
    43981 gtttgtttta tataaccgag cttcataagg tgctagtatg cctttagctt caaattctcc
    44041 gctagaggaa tttgacaaga gtaatgtcca ggactgatct gcttcttttg gaagttcata
    44101 gtcagcaagt gattctgtta agtttaccat aataagatat tgttcatctt ctaaatatct
    44161 ttcatagaca taaagttgtg gatgttcatg aagatattct ttataagaac cgtatatcag
    44221 acctttattg gtttgtctaa tgcggatcat gttcttatag aattgcagga ccgactctgg
    44281 ttcatattct tgttgttcta cattgatcgt tttggtattt ggattagtga tcagccaagg
    44341 ttcatgtgta gaaaaaccag catattgttc cgaagtccat tgcatgggtg tcctgctgtt
    44401 gtctctcgtt gtttcacgaa tgatatccaa tgcttcttct cttgtcttac cttccgcaag
    44461 cagcctttta taaagtcttt tcgaatctac tgcatcaact tgatcgatcg attcaaaggg
    44521 catattcgtc attccgattt cctgtccttg ataaataaat ggcgtacctt gcaaaaagaa
    44581 atacatcata gcgattgctt ttgcagacat agcccaaaaa tcgggacgag tatctccaaa
    44641 cactgaaacg gcacgaggaa catcatggtt ttccatatat aaagcgttcc atccgatacc
    44701 atccaatgaa gtttgccaag cgcttaatgc atgtttcaat ttaaggacat ctaattgccc
    44761 ttctttttct tgctgccaga tatggatatg atcaaattcg aatatcatat cgaaataacc
    44821 gtctttccct acccattgtg gtccttcttc tgcagtgaca ccacttgctt ctccgacagt
    44881 catgatatcg tactcttcaa aaacatgttt caaatcggtc aaatgttctt cgattcctga
    44941 gacattttga aagggtgaga aagggttctc tgtggctttt actgaatatt catctttttt
    45001 tacatgagaa atcgcatcta aacgaaatcc atctatccct ttatccaacc accatcggat
    45061 catattgaat agttcttctt tcaatttctc gctttcccaa tttaagtctg gttgttcttt
    45121 tgcaaaaaca tgcagataat actgtccagt ttcatgagaa tactcccaag cagaaccgcc
    45181 gaaaatcgat gtccaattat tcggttcttt gccatctacc ccgtcgaccc aaatgtaata
    45241 atcgcgatac gggctttctt tagatttttt cgattcttca aaccattgat gctgatccga
    45301 actatggttg atgacaagat ccataatcac tttgatccct aattcatgtg cttcttttag
    45361 caataaatca aaatcttcca tcgttccgaa tctctcttct atatcttgat agtcacttac
    45421 gtcataacca ttatcgacaa atggagattg atagatcgga ttgatccaga taaagccgat
    45481 accaagatct ttcaaataat taagtttttc acggatccca ttgatgtctc cttgaccatc
    45541 gccattgcta tctttgaaac tagcaggata gatttgatag cctactgctt ctttccacca
    45601 tttttgttct tgtatcatat cgtttcactc ctttaacggt ccctcttgat tataaccgat
    45661 tctattttag aagtatcctt gttaccggta actagaaacg aaaagctcaa cgaatctgct
    45721 aagtgcgttc gttgagcttt attttatcat agatagcggg caatcagttg aatcatttgc
    45781 ttgggaaatt cattcaagtc agtaatatct acaaaacgac tttcaccgta gatcgacttg
    45841 atttcttctt tgtcttggcc aatagctgcg gagacaaata ggattccttg acgttcatat
    45901 tctttcaaaa cagactggat atcttctttt gcttttttgc ctgtataatc cggcaatgct
    45961 tttggctgac cgtcactaat attaagcaac agtttggttg ttgcggattg tctgtttaat
    46021 ttttctgctg ctaaacgaag tgcagcacca tcacgattgt tttgccttgg tttcatagtg
    46081 actaattttt catttaacca ttgaaatccg tcttcaaatt ctttataaga aaacaaagaa
    46141 gtcttttctc ttgacgaacg gtctgctgta tctccgtaaa tcaacaatgg gatatcgact
    46201 ttcttagaaa attcggcaat ggcaatcgct gctttttttg ccgcttcgat ccgatcatcc
    46261 cgtatcattg acccagattc atcgatccgt acagctaccg ctaaagaagg ttgctcatgt
    46321 ggcgggttct ttttgtcaaa atttctcaaa tcaccatatg ctactttgct tgcattgaac
    46381 cgctgacctt cgtattttcc tttttggtac gtctctgttt gttcattatc cagtaactct
    46441 aaaatttgcc ggctaagtga ttctactatt ggcattactt gcatgtgtag ctcacgtgct
    46501 tccggcgttt ttccttggaa agctggtcgg tgaacgatga gaccttcttt ttcatgaatc
    46561 gtttccttca tgatttgtcg cgcttcttta ttcagttgct tcttgatttc cttatcatgt
    46621 gcttccatgt cagaagcaga catttcttga gaatcatctc ctgtttgttg ttctttttga
    46681 ttttttcctt cgatatcgct actgcgttct ttttgcattt gaggaggaat ctcgtttttt
    46741 tctattgttt cttcttgttt ctcttctccc tcatttggtt cttcagcaga caacgatttc
    46801 ttttcatcta agcgcagaat ttcttcagca ttttcagtga tgtttcgttc atttgcttct
    46861 tcggcgctaa tggttctttc ttttgtatgc tcagattgag gatgacccca tttttctttt
    46921 ctcaatatgt cctgaagttc cagtagcaat ccactgtcag acaaagcttg ttcaagaatt
    46981 gccaattcat ctgaatcagt ggataattta tacaaaactt ttggataaat cgactggagt
    47041 aaatcttgtc cttgcttata ggtatttgcc caataaaaat aagaacgcat tcctgctaca
    47101 cctttgatgg cgttagcttt tgcagtttca tccaacaaac ggataatctc tgccatcttt
    47161 aagagaagtt cttgtttttg gatcatagtt ttcgccatcg cacgttctgc catgacttct
    47221 aaggcaggaa gatccatttt ttccgcatgt tgcatccgat ctcttaaaga ttcatttaat
    47281 gggcgatttc cttggtaatt tcgatttgta gtgatcacaa tgacgcaatc tggatggcga
    47341 cgaatgattc ctgtcggtaa attcaacatc ccatttggtt cgagcgctga gttaagtgcc
    47401 acaagaacag aggcatctcg gatgacagtc ggctcttgaa tctctaataa atagcctttt
    47461 tcaatcgctc gaacgatttc agaagggtaa tagctgtaat ggattgcatc tggataatca
    47521 gcttcgatcc tttgaactag ctctgctaat ttttctttag cagaaaaagg atcaatgccg
    47581 taatggttct ggatcagtgt cagtacagct tcaagtgtat ctgtttggta aatagcttct
    47641 agtaattctt ggtcttgttc cttattagaa tctactacag gtaataaggc tccgaaaaca
    47701 tctgatttgt ccatatctgc aaaacaggtc acttttgtat agggaagttg cagatcagca
    47761 gatagtgctt tggctaattg tgtcttcccc gacccggcat ctccttccaa aagtacgtta
    47821 ctgattttca tttcaggatc ttgccaattc cctttgattt cggaggcgat ccgccattct
    47881 tctgcacttg tccgatggct tttcggtttc ttccagatca tttgcttttc tagccgtgaa
    47941 aaggttcggt tttctgttag tgagaaattc tccatgcttc tcttcccctt ttgatcatct
    48001 ctgtctatta atatcctagc tcatctaaaa gacgttttaa aatacggaat cgttccccaa
    48061 tcagttctcc gtctttttgt agtgtatcgt aatacaaaat gatgtcttca tcgatctttg
    48121 gatttttggt atctacttct accgtcattc ggttcccttg taatccaatg atgtctacga
    48181 ctggattttc tggatcataa aatttttcat aacgataagc atcttggaag tataaacttc
    48241 cttgcgcagc taagatcgct aaatctaaaa tacgtgtgat tggtaattct tccgactgtc
    48301 gtgaccattt ttctccagta tgtcgccata ccttgcctga gatatcgact tttccacgat
    48361 cattccactg agcaaggccc agtgaaagcc cttttgcatc catatttaat gcatttcttc
    48421 catcaacttt atcatagtct tctacaacga ttactggttt atgttttaaa tttgttggta
    48481 tttccattat tttaactcct catcctttta tttttagtaa ataagtaaat tagtagttta
    48541 ctcatttact aaactatatg cgacattata gaggattgaa gtgaggattg caaagaaaaa
    48601 gagcttcatt gatagaaata aaaacaccat ctcgttcttt tacacgctca ttagtaacat
    48661 gtagaaagtc caagatgatg ttggaat
//
LOCUS       DACECS010000017        46033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.017, whole genome shotgun sequence.
ACCESSION   DACECS010000017 DACECS010000000
VERSION     DACECS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.017"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(64..915)
                     /locus_tag="IWO31_001018"
     CDS             complement(64..915)
                     /locus_tag="IWO31_001018"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0301450.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="IWO31_001019"
     CDS             complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="IWO31_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0301451.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3783..4589
                     /gene="proC"
                     /locus_tag="IWO31_001020"
     CDS             3783..4589
                     /gene="proC"
                     /locus_tag="IWO31_001020"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0301452.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4668..6224)
                     /gene="rny"
                     /locus_tag="IWO31_001021"
     CDS             complement(4668..6224)
                     /gene="rny"
                     /locus_tag="IWO31_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0301453.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6635..7684)
                     /gene="recA"
                     /locus_tag="IWO31_001022"
     CDS             complement(6635..7684)
                     /gene="recA"
                     /locus_tag="IWO31_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0301454.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(7793..9034)
                     /locus_tag="IWO31_001023"
     CDS             complement(7793..9034)
                     /locus_tag="IWO31_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0301455.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(9274..9852)
                     /gene="pgsA"
                     /locus_tag="IWO31_001024"
     CDS             complement(9274..9852)
                     /gene="pgsA"
                     /locus_tag="IWO31_001024"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0301456.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(10061..10945)
                     /locus_tag="IWO31_001025"
     CDS             complement(10061..10945)
                     /locus_tag="IWO31_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301457.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(11723..13009)
                     /locus_tag="IWO31_001026"
     CDS             complement(11723..13009)
                     /locus_tag="IWO31_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0301458.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(13002..14270)
                     /locus_tag="IWO31_001027"
     CDS             complement(13002..14270)
                     /locus_tag="IWO31_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0301459.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            14522..14983
                     /gene="mscL"
                     /locus_tag="IWO31_001028"
     CDS             14522..14983
                     /gene="mscL"
                     /locus_tag="IWO31_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0301460.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(15043..15489)
                     /locus_tag="IWO31_001029"
     CDS             complement(15043..15489)
                     /locus_tag="IWO31_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301461.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(15582..15827)
                     /locus_tag="IWO31_001030"
     CDS             complement(15582..15827)
                     /locus_tag="IWO31_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301462.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(15943..16521)
                     /locus_tag="IWO31_001031"
     CDS             complement(15943..16521)
                     /locus_tag="IWO31_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0301463.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(16598..17128)
                     /locus_tag="IWO31_001032"
     CDS             complement(16598..17128)
                     /locus_tag="IWO31_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0301464.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(17132..17992)
                     /gene="rlmB"
                     /locus_tag="IWO31_001033"
     CDS             complement(17132..17992)
                     /gene="rlmB"
                     /locus_tag="IWO31_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0301465.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(17985..18380)
                     /locus_tag="IWO31_001034"
     CDS             complement(17985..18380)
                     /locus_tag="IWO31_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0301466.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(18377..19789)
                     /locus_tag="IWO31_001035"
     CDS             complement(18377..19789)
                     /locus_tag="IWO31_001035"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0301467.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(19786..20322)
                     /gene="cysE"
                     /locus_tag="IWO31_001036"
     CDS             complement(19786..20322)
                     /gene="cysE"
                     /locus_tag="IWO31_001036"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0301468.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(20644..22104)
                     /locus_tag="IWO31_001037"
     CDS             complement(20644..22104)
                     /locus_tag="IWO31_001037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0301469.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(22274..23431)
                     /locus_tag="IWO31_001038"
     CDS             complement(22274..23431)
                     /locus_tag="IWO31_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0301470.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(23465..24835)
                     /gene="radA"
                     /locus_tag="IWO31_001039"
     CDS             complement(23465..24835)
                     /gene="radA"
                     /locus_tag="IWO31_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0301471.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(24898..25425)
                     /locus_tag="IWO31_001040"
     CDS             complement(24898..25425)
                     /locus_tag="IWO31_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0301472.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            25655..26461
                     /gene="proB"
                     /locus_tag="IWO31_001041"
     CDS             25655..26461
                     /gene="proB"
                     /locus_tag="IWO31_001041"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0301473.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            26458..27705
                     /locus_tag="IWO31_001042"
     CDS             26458..27705
                     /locus_tag="IWO31_001042"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0301474.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            27781..28125
                     /locus_tag="IWO31_001043"
     CDS             27781..28125
                     /locus_tag="IWO31_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301475.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(28242..28568)
                     /locus_tag="IWO31_001044"
     CDS             complement(28242..28568)
                     /locus_tag="IWO31_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301476.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(29183..30385)
                     /locus_tag="IWO31_001045"
     CDS             complement(29183..30385)
                     /locus_tag="IWO31_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301477.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            30502..31017
                     /locus_tag="IWO31_001046"
     CDS             30502..31017
                     /locus_tag="IWO31_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0301478.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(31216..31662)
                     /locus_tag="IWO31_001047"
     CDS             complement(31216..31662)
                     /locus_tag="IWO31_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0301479.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(31709..33187)
                     /gene="msr"
                     /locus_tag="IWO31_001048"
     CDS             complement(31709..33187)
                     /gene="msr"
                     /locus_tag="IWO31_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0301480.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(33541..34170)
                     /locus_tag="IWO31_001049"
     CDS             complement(33541..34170)
                     /locus_tag="IWO31_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0301481.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(34154..34618)
                     /locus_tag="IWO31_001050"
     CDS             complement(34154..34618)
                     /locus_tag="IWO31_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0301482.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(35105..36373)
                     /locus_tag="IWO31_001051"
     CDS             complement(35105..36373)
                     /locus_tag="IWO31_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0301483.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(36604..37341)
                     /locus_tag="IWO31_001052"
     CDS             complement(36604..37341)
                     /locus_tag="IWO31_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0301484.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(37408..37977)
                     /locus_tag="IWO31_001053"
     CDS             complement(37408..37977)
                     /locus_tag="IWO31_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0301485.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            38105..38977
                     /locus_tag="IWO31_001054"
     CDS             38105..38977
                     /locus_tag="IWO31_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0301486.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            39319..40191
                     /locus_tag="IWO31_001055"
     CDS             39319..40191
                     /locus_tag="IWO31_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0301487.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            40188..42146
                     /locus_tag="IWO31_001056"
     CDS             40188..42146
                     /locus_tag="IWO31_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0301488.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(42478..43329)
                     /locus_tag="IWO31_001057"
     CDS             complement(42478..43329)
                     /locus_tag="IWO31_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0301489.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            43452..43919
                     /locus_tag="IWO31_001058"
     CDS             43452..43919
                     /locus_tag="IWO31_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0301490.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(44182..44664)
                     /locus_tag="IWO31_001059"
     CDS             complement(44182..44664)
                     /locus_tag="IWO31_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0301491.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(44764..45627)
                     /locus_tag="IWO31_001060"
     CDS             complement(44764..45627)
                     /locus_tag="IWO31_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0301492.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13130 a         9840 c         8239 g        14824 t
ORIGIN      
        1 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
       61 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
      121 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
      181 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
      241 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
      301 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
      361 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
      421 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
      481 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
      541 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
      601 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
      661 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
      721 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
      781 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
      841 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
      901 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
      961 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
     1021 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
     1081 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
     1141 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
     1201 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
     1261 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
     1321 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
     1381 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
     1441 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
     1501 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
     1561 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
     1621 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
     1681 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
     1741 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
     1801 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
     1861 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
     1921 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
     1981 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
     2041 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
     2101 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
     2161 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
     2221 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
     2281 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
     2341 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
     2401 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
     2461 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
     2521 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
     2581 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
     2641 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
     2701 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
     2761 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa
     2821 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
     2881 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
     2941 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
     3001 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
     3061 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
     3121 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
     3181 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
     3241 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
     3301 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
     3361 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
     3421 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
     3481 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
     3541 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
     3601 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
     3661 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
     3721 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
     3781 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
     3841 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
     3901 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
     3961 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
     4021 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
     4081 tagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
     4141 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
     4201 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
     4261 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
     4321 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
     4381 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
     4441 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
     4501 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
     4561 aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa
     4621 aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa
     4681 tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct
     4741 tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct
     4801 tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca
     4861 ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat
     4921 gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca
     4981 gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact
     5041 gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc
     5101 tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata
     5161 tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc
     5221 aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct
     5281 ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt
     5341 tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt
     5401 ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat
     5461 ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc
     5521 tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc
     5581 ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc
     5641 gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat
     5701 tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca
     5761 tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca
     5821 atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct
     5881 tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc
     5941 gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt actttctctc
     6001 agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct
     6061 tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat
     6121 tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga
     6181 ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata
     6241 ctatcttttt catttttatc atctatacag cttctatgat aaattattct aaactcgtca
     6301 atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta
     6361 ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat
     6421 ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt
     6481 ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa
     6541 ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac
     6601 tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt
     6661 cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa
     6721 ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt
     6781 cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa
     6841 tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca
     6901 tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct
     6961 ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca
     7021 aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa
     7081 acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt
     7141 tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat
     7201 gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga
     7261 ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt
     7321 ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat
     7381 attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta
     7441 cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt
     7501 cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc
     7561 cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt
     7621 taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg
     7681 ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat
     7741 atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc
     7801 ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag
     7861 cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga
     7921 tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag
     7981 ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt
     8041 ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt
     8101 ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt
     8161 cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct
     8221 tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat
     8281 aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc
     8341 ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat
     8401 atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc
     8461 gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa
     8521 aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg
     8581 gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc
     8641 ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt
     8701 tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat
     8761 tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg
     8821 tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca
     8881 actcttctaa acgctgctga ttatctccta caactgtttg ataataaact tcgataccta
     8941 gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa
     9001 gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata
     9061 cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg
     9121 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
     9181 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
     9241 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
     9301 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
     9361 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
     9421 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
     9481 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
     9541 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
     9601 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
     9661 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
     9721 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
     9781 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
     9841 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
     9901 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
     9961 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
    10021 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
    10081 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
    10141 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
    10201 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
    10261 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
    10321 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
    10381 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
    10441 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
    10501 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
    10561 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
    10621 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
    10681 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
    10741 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
    10801 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
    10861 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
    10921 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
    10981 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
    11041 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
    11101 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
    11161 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
    11221 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
    11281 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
    11341 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
    11401 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
    11461 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    11521 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
    11581 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
    11641 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
    11701 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
    11761 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
    11821 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
    11881 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
    11941 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
    12001 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
    12061 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
    12121 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
    12181 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
    12241 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
    12301 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
    12361 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
    12421 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
    12481 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
    12541 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
    12601 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
    12661 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
    12721 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
    12781 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
    12841 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
    12901 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
    12961 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
    13021 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
    13081 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
    13141 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
    13201 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
    13261 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
    13321 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
    13381 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
    13441 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
    13501 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
    13561 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
    13621 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
    13681 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
    13741 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
    13801 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
    13861 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
    13921 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
    13981 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
    14041 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
    14101 tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
    14161 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
    14221 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
    14281 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
    14341 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
    14401 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
    14461 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
    14521 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
    14581 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
    14641 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
    14701 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
    14761 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
    14821 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
    14881 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
    14941 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
    15001 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
    15061 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
    15121 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
    15181 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
    15241 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
    15301 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc
    15361 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
    15421 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
    15481 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
    15541 aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa
    15601 aattcgatct caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa
    15661 gaattttctt caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg
    15721 ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg
    15781 atacgacctt ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct
    15841 actatcaact ataccacata tttttaaaat tttcaagttt accataaaaa aaaaaagaaa
    15901 tgcaataaaa ataaataaga agctcattaa gatttttcta aattattcct cattataaaa
    15961 tttcaagtgt ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg
    16021 atccattgct aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa
    16081 aattttagct tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa
    16141 aagatgctga attgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc
    16201 tgtctctatt ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt
    16261 ctgcttcctc aacagagaat agatttgatt gcttataacg cttttataat aacttccaaa
    16321 gctaacacct ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc
    16381 ttgttcccaa tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa
    16441 aagtggaaaa tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc
    16501 gcgcaataac tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt
    16561 atcatcaatg attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat
    16621 cgtccaattt ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga
    16681 aatcaataaa atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg
    16741 tcttatataa ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt
    16801 ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga
    16861 ttctctcgat ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt
    16921 atttcttata attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt
    16981 ccattccttc atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg
    17041 ccattttgtc ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc
    17101 cgtctacaat caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct
    17161 gatacataag caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg
    17221 ggatcgtcag caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat
    17281 taccaatgac taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca
    17341 tatctgtgcc aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat
    17401 tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag
    17461 gtgtgattcc aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg
    17521 ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga
    17581 aggggtcttc ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat
    17641 aaggtgtaat cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt
    17701 tttgtttagg aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt
    17761 ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta
    17821 gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt
    17881 gatcagaaac tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt
    17941 tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca
    18001 ccaactttct gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa
    18061 tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca
    18121 ttttttgcgc tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct
    18181 tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa
    18241 acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg
    18301 tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc
    18361 aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta
    18421 ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat
    18481 tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt
    18541 ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg
    18601 ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct
    18661 catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc
    18721 gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg
    18781 cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg
    18841 ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca
    18901 tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat
    18961 tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca
    19021 tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat
    19081 gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt
    19141 ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat
    19201 cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt
    19261 tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt
    19321 ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag
    19381 attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat
    19441 gatccatgac tcttggatgc agtgtagcag gttcgacatt caacgctttc gtatcttctt
    19501 caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg
    19561 tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc
    19621 gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat
    19681 ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag
    19741 ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct
    19801 cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc
    19861 tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac
    19921 cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct
    19981 tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc
    20041 ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt
    20101 tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt
    20161 gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa
    20221 caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc
    20281 gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg
    20341 taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt
    20401 ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct
    20461 ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc
    20521 ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata
    20581 ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg
    20641 tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat
    20701 cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa
    20761 aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt
    20821 tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg
    20881 aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa
    20941 aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct
    21001 aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc
    21061 agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta
    21121 atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg
    21181 ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg
    21241 tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg
    21301 atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc
    21361 actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata
    21421 gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat
    21481 tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt
    21541 ttggatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat
    21601 atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc
    21661 ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg
    21721 aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca
    21781 atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc
    21841 tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca
    21901 attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg
    21961 tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa
    22021 ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc
    22081 atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac
    22141 agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca
    22201 aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg
    22261 ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga
    22321 ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg
    22381 cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa
    22441 ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta
    22501 ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat
    22561 tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg
    22621 ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga
    22681 tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat
    22741 ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga
    22801 tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag
    22861 tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt
    22921 tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt
    22981 cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg
    23041 ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat
    23101 tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc
    23161 cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt
    23221 gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga
    23281 aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt
    23341 gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga
    23401 tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta
    23461 ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga
    23521 ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc
    23581 cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag
    23641 tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg
    23701 aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg
    23761 cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg
    23821 ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga
    23881 acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg
    23941 ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag
    24001 gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg
    24061 agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa
    24121 aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt
    24181 tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcggtt ttgagtagtt
    24241 ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg
    24301 tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac
    24361 tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc
    24421 gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc
    24481 cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg
    24541 gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca
    24601 gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg
    24661 ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt
    24721 cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag
    24781 gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac
    24841 cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta
    24901 tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt
    24961 taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt
    25021 gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata
    25081 atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct
    25141 tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg
    25201 cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc
    25261 gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc
    25321 tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt
    25381 ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta
    25441 aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt
    25501 caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa
    25561 gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg
    25621 aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa
    25681 acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct
    25741 tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt
    25801 gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg
    25861 tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa
    25921 tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag
    25981 agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt
    26041 gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga
    26101 tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc
    26161 ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac
    26221 aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca
    26281 agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc
    26341 ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat
    26401 tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg
    26461 actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg
    26521 cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa
    26581 caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt
    26641 gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag
    26701 atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac
    26761 accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac
    26821 gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc
    26881 gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg
    26941 cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact
    27001 tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct
    27061 cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa
    27121 acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg
    27181 aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg
    27241 ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa
    27301 cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta
    27361 gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc
    27421 gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg
    27481 atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct
    27541 gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag
    27601 attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact
    27661 tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac
    27721 attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat
    27781 atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt
    27841 ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa
    27901 cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg
    27961 cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc
    28021 gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat
    28081 aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga
    28141 taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa
    28201 gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa
    28261 tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga
    28321 agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc
    28381 agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta
    28441 gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga
    28501 tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct
    28561 tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa
    28621 attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc
    28681 cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc acaatattca
    28741 acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc
    28801 tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc tcaaaaaacg
    28861 gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat aaaacgctga
    28921 ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac
    28981 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac aaaacgctga
    29041 ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    29101 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag
    29161 ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat
    29221 aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt
    29281 caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc
    29341 ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa
    29401 tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg
    29461 gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga
    29521 caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga
    29581 aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt
    29641 cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat
    29701 ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt
    29761 cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta
    29821 taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc
    29881 ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca
    29941 tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag
    30001 agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat
    30061 tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg
    30121 tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga
    30181 ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag
    30241 caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag
    30301 ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat
    30361 ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt
    30421 ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat
    30481 aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta
    30541 tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat
    30601 cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg
    30661 gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt
    30721 gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca
    30781 aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga
    30841 gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta
    30901 gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag
    30961 caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg
    31021 ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca
    31081 atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac
    31141 tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca
    31201 aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg
    31261 gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat
    31321 ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat
    31381 acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct
    31441 ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac
    31501 atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg
    31561 aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat
    31621 cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa
    31681 atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt
    31741 tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag
    31801 aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg
    31861 ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa
    31921 gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca
    31981 aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact
    32041 ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt
    32101 gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga
    32161 tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac
    32221 ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga
    32281 cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac
    32341 ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta
    32401 atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca
    32461 accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg
    32521 cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact
    32581 ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag gcactataat
    32641 tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat
    32701 ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt
    32761 caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc
    32821 cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc
    32881 atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta
    32941 ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt
    33001 ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac
    33061 cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa
    33121 tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat
    33181 tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga
    33241 gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa
    33301 ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa
    33361 tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt
    33421 tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta
    33481 ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt
    33541 ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac
    33601 cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc
    33661 ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg
    33721 cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat
    33781 ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac
    33841 ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc catattcgta
    33901 acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt
    33961 gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg
    34021 ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat
    34081 ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat
    34141 aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta
    34201 tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat
    34261 cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca caatgcttga
    34321 ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc
    34381 tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt
    34441 gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc
    34501 tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga
    34561 ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa
    34621 tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa
    34681 agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag
    34741 agaaaagaaa ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa
    34801 ccaagattat tgattcttat caaactatag aagatagctc ttctgcagta gataatttta
    34861 caccgccaaa aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg
    34921 atcagtatgc ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg
    34981 agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg
    35041 aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct
    35101 ctttttaata accataaaat cctattcctt catatctcca gcccatcggt gttagccaat
    35161 ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata
    35221 gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga
    35281 tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag
    35341 gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat
    35401 attcccagcc tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt
    35461 ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat
    35521 actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag
    35581 tggcataacg acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc
    35641 atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt
    35701 tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc
    35761 attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt
    35821 taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt
    35881 caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt
    35941 gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat
    36001 aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca
    36061 atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag
    36121 cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt
    36181 cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt
    36241 caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag
    36301 ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat
    36361 tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa
    36421 ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa
    36481 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
    36541 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
    36601 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
    36661 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
    36721 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
    36781 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
    36841 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
    36901 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
    36961 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
    37021 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
    37081 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
    37141 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
    37201 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
    37261 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
    37321 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
    37381 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
    37441 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
    37501 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
    37561 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
    37621 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
    37681 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
    37741 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
    37801 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
    37861 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
    37921 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
    37981 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
    38041 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
    38101 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
    38161 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
    38221 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
    38281 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
    38341 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
    38401 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
    38461 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
    38521 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
    38581 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
    38641 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
    38701 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
    38761 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
    38821 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
    38881 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
    38941 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
    39001 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
    39061 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
    39121 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
    39181 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
    39241 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
    39301 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
    39361 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
    39421 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
    39481 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
    39541 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
    39601 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
    39661 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
    39721 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
    39781 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
    39841 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
    39901 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
    39961 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
    40021 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
    40081 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
    40141 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
    40201 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
    40261 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
    40321 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
    40381 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
    40441 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
    40501 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
    40561 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
    40621 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
    40681 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
    40741 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
    40801 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
    40861 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
    40921 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
    40981 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
    41041 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
    41101 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
    41161 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
    41221 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
    41281 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
    41341 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
    41401 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
    41461 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
    41521 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
    41581 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
    41641 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
    41701 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
    41761 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
    41821 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
    41881 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
    41941 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
    42001 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
    42061 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
    42121 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
    42181 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
    42241 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
    42301 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
    42361 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
    42421 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
    42481 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
    42541 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
    42601 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
    42661 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
    42721 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
    42781 tactagtact tttgctgcag cttctgcata ttcacgtttc aatgcccaac ctgtttttcc
    42841 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
    42901 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
    42961 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
    43021 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
    43081 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
    43141 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
    43201 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
    43261 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
    43321 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
    43381 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
    43441 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
    43501 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
    43561 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
    43621 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
    43681 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
    43741 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
    43801 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
    43861 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
    43921 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
    43981 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
    44041 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
    44101 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
    44161 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
    44221 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
    44281 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
    44341 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
    44401 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
    44461 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
    44521 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
    44581 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
    44641 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
    44701 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
    44761 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
    44821 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
    44881 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
    44941 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
    45001 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
    45061 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
    45121 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
    45181 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
    45241 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
    45301 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
    45361 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
    45421 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
    45481 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
    45541 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
    45601 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
    45661 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
    45721 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
    45781 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
    45841 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
    45901 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
    45961 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
    46021 cagataaata aaa
//
LOCUS       DACECS010000018        44938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.018, whole genome shotgun sequence.
ACCESSION   DACECS010000018 DACECS010000000
VERSION     DACECS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.018"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWO31_001061"
     CDS             complement(38..1189)
                     /locus_tag="IWO31_001061"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0301493.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWO31_001062"
     CDS             complement(1202..1639)
                     /locus_tag="IWO31_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0301494.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWO31_001063"
     CDS             complement(1654..3420)
                     /locus_tag="IWO31_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0301495.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWO31_001064"
     CDS             3684..4361
                     /locus_tag="IWO31_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301496.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IWO31_001065"
     CDS             4364..5491
                     /locus_tag="IWO31_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301497.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IWO31_001066"
     CDS             complement(5691..6131)
                     /locus_tag="IWO31_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0301498.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IWO31_001067"
     CDS             complement(6142..8175)
                     /locus_tag="IWO31_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0301499.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IWO31_001068"
     CDS             complement(8177..9880)
                     /locus_tag="IWO31_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0301500.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWO31_001069"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWO31_001069"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0301501.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IWO31_001070"
     CDS             complement(10963..11988)
                     /locus_tag="IWO31_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0301502.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IWO31_001071"
     CDS             complement(12197..13066)
                     /locus_tag="IWO31_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0301503.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IWO31_001072"
     CDS             complement(13525..15417)
                     /locus_tag="IWO31_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301504.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IWO31_001073"
     CDS             complement(15414..17141)
                     /locus_tag="IWO31_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301505.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IWO31_001074"
     CDS             complement(17157..17807)
                     /locus_tag="IWO31_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0301506.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IWO31_001075"
     CDS             18011..18307
                     /locus_tag="IWO31_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0301507.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IWO31_001076"
     CDS             18479..19099
                     /locus_tag="IWO31_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0301508.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IWO31_001077"
     CDS             complement(19191..20357)
                     /locus_tag="IWO31_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0301509.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IWO31_001078"
     CDS             complement(20369..20722)
                     /locus_tag="IWO31_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0301510.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IWO31_001079"
     CDS             20850..21872
                     /locus_tag="IWO31_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0301511.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IWO31_001080"
     CDS             complement(22311..22745)
                     /locus_tag="IWO31_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301512.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IWO31_001081"
     CDS             complement(22855..24714)
                     /locus_tag="IWO31_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0301513.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IWO31_001082"
     CDS             complement(24832..25992)
                     /locus_tag="IWO31_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0301514.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWO31_001083"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWO31_001083"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0301515.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IWO31_001084"
     CDS             complement(27739..28437)
                     /locus_tag="IWO31_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0301516.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IWO31_001085"
     CDS             complement(28394..30055)
                     /locus_tag="IWO31_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0301517.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IWO31_001086"
     CDS             30273..31673
                     /locus_tag="IWO31_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0301518.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IWO31_001087"
     CDS             complement(31925..33004)
                     /locus_tag="IWO31_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0301519.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IWO31_001088"
     CDS             complement(33090..34049)
                     /locus_tag="IWO31_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0301520.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IWO31_001089"
     CDS             complement(34061..35584)
                     /locus_tag="IWO31_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0301521.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IWO31_001090"
     CDS             complement(35594..36157)
                     /locus_tag="IWO31_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0301522.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IWO31_001091"
     CDS             complement(36163..37026)
                     /locus_tag="IWO31_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0301523.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IWO31_001092"
     CDS             complement(37040..37936)
                     /locus_tag="IWO31_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0301524.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IWO31_001093"
     CDS             complement(38111..39382)
                     /locus_tag="IWO31_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0301525.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IWO31_001094"
     CDS             complement(39372..40889)
                     /locus_tag="IWO31_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0301526.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IWO31_001095"
     CDS             41087..41977
                     /locus_tag="IWO31_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0301527.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IWO31_001096"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IWO31_001096"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0301528.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="IWO31_001097"
     CDS             complement(42886..44358)
                     /locus_tag="IWO31_001097"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0301529.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14779 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttt
//
LOCUS       DACECS010000019        41958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.019, whole genome shotgun sequence.
ACCESSION   DACECS010000019 DACECS010000000
VERSION     DACECS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.019"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            130..1566
                     /locus_tag="IWO31_001098"
     CDS             130..1566
                     /locus_tag="IWO31_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0301530.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1755..4202
                     /locus_tag="IWO31_001099"
     CDS             1755..4202
                     /locus_tag="IWO31_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0301531.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4432..5499
                     /gene="rlmN"
                     /locus_tag="IWO31_001100"
     CDS             4432..5499
                     /gene="rlmN"
                     /locus_tag="IWO31_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0301532.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5692..6795)
                     /locus_tag="IWO31_001101"
     CDS             complement(5692..6795)
                     /locus_tag="IWO31_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0301533.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7348..7896
                     /gene="aac(6')"
                     /locus_tag="IWO31_001102"
     CDS             7348..7896
                     /gene="aac(6')"
                     /locus_tag="IWO31_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0301534.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            8442..10103
                     /locus_tag="IWO31_001103"
     CDS             8442..10103
                     /locus_tag="IWO31_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0301535.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(10173..10520)
                     /locus_tag="IWO31_001104"
     CDS             complement(10173..10520)
                     /locus_tag="IWO31_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0301536.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            10604..11545
                     /locus_tag="IWO31_001105"
     CDS             10604..11545
                     /locus_tag="IWO31_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301537.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            11546..12589
                     /locus_tag="IWO31_001106"
     CDS             11546..12589
                     /locus_tag="IWO31_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301538.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            12595..13665
                     /locus_tag="IWO31_001107"
     CDS             12595..13665
                     /locus_tag="IWO31_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301539.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            13655..14608
                     /locus_tag="IWO31_001108"
     CDS             13655..14608
                     /locus_tag="IWO31_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301540.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            14620..15630
                     /locus_tag="IWO31_001109"
     CDS             14620..15630
                     /locus_tag="IWO31_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0301541.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(15915..16736)
                     /locus_tag="IWO31_001110"
     CDS             complement(15915..16736)
                     /locus_tag="IWO31_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0301542.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(16833..17111)
                     /locus_tag="IWO31_001111"
     CDS             complement(16833..17111)
                     /locus_tag="IWO31_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301543.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            17379..19388
                     /gene="metG"
                     /locus_tag="IWO31_001112"
     CDS             17379..19388
                     /gene="metG"
                     /locus_tag="IWO31_001112"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0301544.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(19867..20454)
                     /locus_tag="IWO31_001113"
     CDS             complement(19867..20454)
                     /locus_tag="IWO31_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301545.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            20629..21951
                     /locus_tag="IWO31_001114"
     CDS             20629..21951
                     /locus_tag="IWO31_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0301546.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            22106..22882
                     /locus_tag="IWO31_001115"
     CDS             22106..22882
                     /locus_tag="IWO31_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0301547.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            22875..23444
                     /gene="rnmV"
                     /locus_tag="IWO31_001116"
     CDS             22875..23444
                     /gene="rnmV"
                     /locus_tag="IWO31_001116"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0301548.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            23437..24321
                     /gene="rsmA"
                     /locus_tag="IWO31_001117"
     CDS             23437..24321
                     /gene="rsmA"
                     /locus_tag="IWO31_001117"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0301549.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            24772..25401
                     /locus_tag="IWO31_001118"
     CDS             24772..25401
                     /locus_tag="IWO31_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301550.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(25525..26634)
                     /gene="ugpC"
                     /locus_tag="IWO31_001119"
     CDS             complement(25525..26634)
                     /gene="ugpC"
                     /locus_tag="IWO31_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0301551.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            26843..27265
                     /gene="mgsA"
                     /locus_tag="IWO31_001120"
     CDS             26843..27265
                     /gene="mgsA"
                     /locus_tag="IWO31_001120"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0301552.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            27588..28124
                     /locus_tag="IWO31_001121"
     CDS             27588..28124
                     /locus_tag="IWO31_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0301553.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(28164..29927)
                     /locus_tag="IWO31_001122"
     CDS             complement(28164..29927)
                     /locus_tag="IWO31_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301554.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(29930..31645)
                     /locus_tag="IWO31_001123"
     CDS             complement(29930..31645)
                     /locus_tag="IWO31_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301555.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(32424..32648)
                     /locus_tag="IWO31_001124"
     CDS             complement(32424..32648)
                     /locus_tag="IWO31_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301556.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            32848..35616
                     /locus_tag="IWO31_001125"
     CDS             32848..35616
                     /locus_tag="IWO31_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0301557.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            35697..36116
                     /locus_tag="IWO31_001126"
     CDS             35697..36116
                     /locus_tag="IWO31_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0301558.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            36128..36598
                     /locus_tag="IWO31_001127"
     CDS             36128..36598
                     /locus_tag="IWO31_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0301559.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            36615..37394
                     /locus_tag="IWO31_001128"
     CDS             36615..37394
                     /locus_tag="IWO31_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0301560.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            37381..38202
                     /locus_tag="IWO31_001129"
     CDS             37381..38202
                     /locus_tag="IWO31_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0301561.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            38219..39268
                     /locus_tag="IWO31_001130"
     CDS             38219..39268
                     /locus_tag="IWO31_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0301562.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            39281..40285
                     /locus_tag="IWO31_001131"
     CDS             39281..40285
                     /locus_tag="IWO31_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0301563.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            40918..41937
                     /locus_tag="IWO31_001132"
     CDS             40918..41937
                     /locus_tag="IWO31_001132"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0301564.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        13761 a         7378 c         8672 g        12147 t
ORIGIN      
        1 tttttcttac tactcgaaac taagcgactt tttcacaacc tcctttacat aattttattt
       61 tgttgtggaa ggggtttcgt gttattctaa aagaggaacg attgaaggtt ataggaaagg
      121 aagagatgaa tggaacacaa agaacttacc tcatttccaa aagattttct gtggggctct
      181 gcttctgcag cgtatcaagt agaaggagct tggcaagaag atgggaaagg agaatcagtc
      241 tgggatcgat ttgtacggat acctggaaag acatttaaag gaacgaacgg tgatttggca
      301 gtagaccact atcatcgtta taaagaagat attgctttaa tgaaagaaca aggtctgaaa
      361 gcttaccgct tctctgtagc ttggacacgg atttttccta atggacgagg agaagtcaat
      421 caagctgggc tcgcttttta tgagagattg atcgacgaat tgattgaaaa cgatattgaa
      481 cctatgttga ctttatatca ttgggatctt ccacaagcat tgcaagatga atataatggt
      541 tgggaatcac gtcagatcat tgaagatttc actcattatg cagaaacatt atttgaggca
      601 ttccgcggga aagtccatta ctggattagt ttgaatgaac aaaatatctt cacttcatta
      661 ggttatttgt tagcagcaca tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt
      721 aaccatattg ctaatttagc aaatgcatct gtcatcaata aatttcacga gttagaaatg
      781 cctggaaaaa tcggaccaag ctttgcgtat acgccaaatt atccaattga cagtaatcct
      841 gaaaatgttc tggctgctga aaatgcagaa gacctgatgg ccaattattg gatggatgtc
      901 tatatgtggg gaaaatatcc gattgctgcc atgaggttct tagaagaaaa aggatgggca
      961 ccaacgattg aagctggcga tgcagaattg ctagaatctg ctaaaccaga ttttcttggg
     1021 atcaattact atcaaacggc gacgaatgca ttcaatccat taaacggagt tggtgctgga
     1081 aaaatgaata cgacaggaaa aaaaggttca tcagaagaaa ctggagttcc tggaatgtat
     1141 aaaaaagttg agaatccatt tgtcgaacga acaaattggg actgggagat tgacccagaa
     1201 ggattaagga tcggactgcg tcgtatcaca agccgttacc gcatgccggt actgattacg
     1261 gaaaatggct taggagagta tgacaagttg acagaagata aacaaatcca tgacgactat
     1321 cgaatcaatt acttacaaag ccacgtcaaa gccatcaaag aagcaatttc ggatggagca
     1381 gaagtcctag gctattgcac ctggtcttat acagatttgc ttagctggct gaacggttat
     1441 caaaagcgat atggatttgt atatgttgat caagacgaaa cacaagaagg atctttggaa
     1501 cgatataaga aagacagtta ttactggtac caaaaactga ttgaagaaaa tggagagaac
     1561 ctttagttca catacttaaa acaagaaggt acggctggat atggccgtgc cttttcttga
     1621 taaaatcatt tagaaattaa gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc
     1681 agttctttca aacaaaactt tagttccctt actttttttt gtataatgaa acttgatgaa
     1741 atgagggata ataaatggca caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa
     1801 aaacgaaaaa gcaaaaacag cagcaggaac aaatgctgac gattggtgtt gggtttgcct
     1861 tgattttatt tgctgtcttc ggctttttaa aattaggttt tttaggaata ttgattgcaa
     1921 acggctttcg gatcattgca ggaaatactt accaaatcct ctgtctttta ttagccgtat
     1981 taggattttg gattgtcatt aagaatacag agttttcaat tggtaaaaac cgacgctggt
     2041 ttggcggcat actcttttat ttcggtatct tattattgtt acatgcccat ttatttggca
     2101 aactccatac tggggaacca aatatcatgg ggacgacatg ggatttgtta gccagtgata
     2161 taaaacaaag tcaagtagac aataacgttg gtggtgggat gattggagca atcctttatc
     2221 attttactta cttcttgatt gcacagcctg gaagttattt agcagcagtg ctattactgg
     2281 caggaggagc attcctgttc agtaatctgg aaggctatca gctgttaaat ggtatccaat
     2341 caattggaga acgagtacag gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc
     2401 aagcggcaaa ggaagagaga atgaaacagc gcgcagaagc gaaagaagca aggcgcttag
     2461 ctgcaaaaga agctgcagaa aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc
     2521 agcaaaggga aaatactgct gatgaatggc aggaaccaga aaatacagag ccggagcagc
     2581 taagttttgt tccaatcgac agttttcaag aaaatatcca tcctgcgaat ctagagaaac
     2641 cagtaccaga tacaccaaaa cagaccaata cagcagaagg tttttcagat gagttgccag
     2701 aggatgacgg tacctcatta gaatttgaga ttgaagcaga acaggaaaat caagactatg
     2761 aattaccgac agttgatttg cttgattcta taccaactgt ggatcaaagt gatgagtata
     2821 aaaaaattga aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg
     2881 ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa attcgaagtc cagccggctg
     2941 taggggtaaa agtaagtaaa attgtgaact taacagatga tatcgcgctt gctttagcgg
     3001 cgaaagatgt ccggatggaa gcaccgattc caggtaaatc actgattggt attgaagtac
     3061 caaatggaaa gatcagtatg gtttccttta gagaaatcat cgaagcacaa ccaaaccacc
     3121 cagataaatt actagaagta cctttaggac gtgatgtttc tggacgggta caaacggctg
     3181 atctttcgaa gatgcctcac ttactcgtgg ctggttcgac tggtagtggg aagtcagtag
     3241 caattaatgg gattatcaca agtatattaa tgcgtgctaa gccccatgaa gtaaaactga
     3301 tgatgatcga tccgaaaatg gttgaattga atatgtacaa cggcatacct catctgttga
     3361 cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa
     3421 tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct ggttacaatg
     3481 aatttgttca acagaaaaac ttggaaaacg gaacgaaaca tccaacctta ccattcatcg
     3541 tagtgatcgt agatgaatta gctgatttga tgatggtagc cagcaatgaa gttgaagatg
     3601 cgattattcg tttagcgcaa atggctcggg cagcggggat tcatatgatc ctagctactc
     3661 aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct tccagaatgg
     3721 catttgcggt atcaagcggt acagattctc ggacgatcat tgattctaat ggggcagaaa
     3781 agctcttagg gcgaggagat atgctattct tgccaatggg tgagaataaa ccgatccgtg
     3841 tgcaaggagc attcatttct gaccacgaag tagaacgggt cgttcaattt gttacagatc
     3901 agcaagaagc tcattatgaa gagaaaatga tgccgacaga tgaagttgaa acgacaggtg
     3961 ctccagaaca gccacaagat gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc
     4021 aaacggcaag tatttccctt ctccaaagaa gatttcggat cggttacaac cgagcagcac
     4081 gtttagtaga tgaactggaa gctcatggag ttgttggccc gtcagaagga agcaagcctc
     4141 ggaaagtatt cattgagcag gaagaaacat cagaaacgtt gccggacgat aatgacatgt
     4201 aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga
     4261 taccaaaaga aaaaatggtt tttttccatc ttttctaata cggtatgatg tcttgtcttt
     4321 ttagtttttg tgagattaaa gttctattcg atttatttgt gaagtattgc attaaggaaa
     4381 tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa
     4441 cagtccattt atggcttgac gaatgaagaa ttaatcaatt ggtttataga aaacggcgag
     4501 aaaaaattcc gagctgccca agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc
     4561 acagaaatga cgaatctatc gaaacaattg atcgaaaaat tatcggagca ttttatcatc
     4621 aatcctctaa aacagatggt cgtccaagaa gcttcggatg gtacagtaaa atatttgttt
     4681 gaactccctg ataaaaacat gattgagact gtactgatga gacaagaata tggcttgtca
     4741 gtttgtgtga cgacacaagt aggatgtaat atcggctgta cgttttgtgc aagcgggctg
     4801 ctcaaaaaga accgtgatct gactgctgga gaaattgttg cacagatcat gatggtccaa
     4861 cattattttg atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc
     4921 ggtgagccat ttgataatta cgacaatgta atggactttt tgcatatcat caatgatgcc
     4981 aaaggattgg caattggtgc tcggcacatt acagtttcca ctagtggatt agcacacaaa
     5041 atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg
     5101 aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg
     5161 atggaagctg tggatgagta tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc
     5221 atgttgaatc aagtaaatga tagaccagag catgcgcaac aattagcaga tctattaaaa
     5281 gataagaaaa aattagcata tgtcaacttg attccatata atccagtcag tgaacatgat
     5341 caatattctc gcagtccaaa agctgatgta ttgagatttt atgatgtatt gaagaaaaac
     5401 ggcgtaaact gtgtgatacg taaagaacat ggtacagata ttgatgcagc ttgcggacaa
     5461 ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat cgtatcctac tcgttttttc
     5521 aattaccata aaaggagcag ttttcttaat taaggaactg ttcaacttaa ataagagaaa
     5581 agattctata gcgaaagcta taaagctttt tttgacaaga aaaaccctca tcaagcaaag
     5641 aaatgtcctc aatttgatga cagcctgctt gatgggggtt ttagcaatta tttaactgat
     5701 tttcttatat ccttgctggc tttcttgtgt agcatgataa gtttctacgt caagaatggc
     5761 ctgtttaaat ttttctcggg tgatttcata aggcacatgt ttaatatcat ttgttgccat
     5821 ggctttatct aagattgctt ctaaatcggt cgtgacattg agattcatgt cggttaacga
     5881 tactggaagt tggttctttt tcataaacac atagatttta tcaaacaaat cgaactgctg
     5941 atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa ggtatccctc
     6001 cattttttct agaacagtcg taccgtaata gaatgagtgt gccatagcag aattatagtt
     6061 attgttaact aaaacagaaa ccaatccggt agtaacaatg atatccgaaa cgacttgttc
     6121 aattgcatat gagggacgat cgtcggcact atctttcaat gcttgcgtac cataagtcaa
     6181 gagacgatca atgcacattt ttccgatttg gattcctaat ctatccgtgt agcctagatc
     6241 atcgccacga gcagaaaaag tagattcgac ttctttggat aaagcatcgc caatacctgc
     6301 ccataagtat ttatcaggtg cttcggcaat aattgctgta tcaatgaagg tgtgtacagg
     6361 tggttttgaa ggaaagaaga gtccgtccat tacgccttct tcgttataga tgacacatac
     6421 cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg gtttagctag
     6481 tttttcacca atgactttta cagtatcgca agcacggcca ccaccgactg caaacaggac
     6541 gtctgctttt tggacacttt catgctggat caactgttca atatttccat aagttgaatt
     6601 acccccgtac cagagactgg cgacaacctc tatttcctgt gtcaaagcta cttttatttt
     6661 gttttctgcc tttgaaagtg cagtcttccc tccaatcagc actacttttg tcccatattc
     6721 tttgacgatt ccatttgctt tttcatacgc atctgttcca atggtataat ttggtaagaa
     6781 aacactttga ctcatattca tttccttctt tcttcgttga aacgggttaa aattttaaag
     6841 agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg aacctaagac aggataagtg
     6901 atgaataagg tggcatgaac accttgaaat catcaataag tatcctaaaa acgaacaagt
     6961 acaaaaacat tgaaaacgac aataatgaat ttttgttcct cttagtattt ctaccgattc
     7021 cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta agattattat atgaaaacag
     7081 gttttatttg tcaattcata attggttttt tttaaagcaa aagataatct atatcatgat
     7141 gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta ttttgaataa
     7201 agtgtttctg tataattttt ttattttata agtaagataa ataatgaaag aatagagata
     7261 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
     7321 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
     7381 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
     7441 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
     7501 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
     7561 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
     7621 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
     7681 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
     7741 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
     7801 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
     7861 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
     7921 atcagcttca agaaataaga aggaatttac gaaaattact tcttgaattt ttgtgaaatt
     7981 ccaacttatt tccgaagaag ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt
     8041 cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa
     8101 atttggctta ataccgagaa actggctttt gaacaccgtt tattcagata agggactgtg
     8161 acaagacttt tgtcacagtt tctttttgtt tattcttttt tatctttgta tggcaataat
     8221 agacaatata acgaaaatta tttaatggct tttggaagac catggaaaac cttgtacaaa
     8281 gctatttttt ctgaaaaatt ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag
     8341 acaaaaaaaa gtgttgattt tttaataatt attcattaaa ctcttaaaca acttcagaaa
     8401 atattaatta ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat
     8461 ttggtttggt tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca
     8521 caagcagtag ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg
     8581 tgcctcaaga aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag
     8641 cattgaataa cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg
     8701 caggagctag cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac
     8761 gagaagacgc aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct
     8821 ggcaacgaac agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg
     8881 aaaatgcaga agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag
     8941 ttggtgacta tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc
     9001 ttgctttccc atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct
     9061 atgcatctgc aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg
     9121 gaccaggaac agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata
     9181 cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact
     9241 tattcaaaga tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg
     9301 caaacgatcc agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc
     9361 aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta
     9421 tcgatcgtga agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat
     9481 tgatcccatc agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg
     9541 gaaaattagt ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag
     9601 aattgggcgt tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa
     9661 aagtaatcga atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac
     9721 caacacctgt tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag
     9781 tactaggcgg atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca
     9841 caggtaattc atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag
     9901 cagcttcatc aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa
     9961 tcaagatcat ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc
    10021 tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca
    10081 aatggacata cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc
    10141 ggcaaatgtc gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa
    10201 atagcagaaa gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag
    10261 ctggcagacg agagcgagga aaattctggt tttttctttc ggttccttgt tcgtgcaaga
    10321 tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg
    10381 ataatcagaa acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag
    10441 gaatggttga tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag
    10501 gggatagatt tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt
    10561 agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa
    10621 atatgtatta aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac
    10681 ctttttcttg atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc
    10741 tgaaacaatc gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata
    10801 tgggatttat ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa
    10861 ccagccagtc gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca
    10921 ggcaattatc tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca
    10981 aaatacatgg gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa
    11041 ctttgtattt gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat
    11101 cgcaaaatgg gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc
    11161 gcttgctgac tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta
    11221 tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct
    11281 tcgaaatagt ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac
    11341 cggatcactg gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa
    11401 gtcaatcaca acaaatgatt atcctacgat catggctgta acgatccttt actcatttat
    11461 gctgattttt gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg
    11521 tgtttcagaa gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc
    11581 agatatccct gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca
    11641 gattactgca ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa
    11701 agctgccgtt atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat
    11761 ctttgtttct cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg
    11821 tattccggga atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt
    11881 tgataaatat gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg
    11941 tttgggacgt gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc
    12001 atttattgct gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt
    12061 gttaggcgga cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc
    12121 aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt
    12181 ggctgctatg gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat
    12241 gaaattaaaa gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa
    12301 aattgcattc aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt
    12361 tagtattcca tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac
    12421 gcctccgtca gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct
    12481 gccttatcta ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt
    12541 acttgctgat ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg
    12601 aaaattttag aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga
    12661 gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa
    12721 tctggaagtg gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc
    12781 aatatagatg ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga
    12841 gagatgcaaa aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct
    12901 ttagatccga cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa
    12961 gtttcaaaaa aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc
    13021 gaagctgaga aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg
    13081 atcgtgatcg cgattgcact ggtatgttat ccggaaatcc tgattgctga tgaacctaca
    13141 acagcgcttg atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca
    13201 aaaatcgata catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct
    13261 gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt
    13321 ttctacaacc cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat
    13381 agtgcgaacg atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca
    13441 aaaggagacg cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa
    13501 ccaccatttt ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag
    13561 gcacccaaag tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa
    13621 caaaaacata cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa
    13681 aggattgaaa cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat
    13741 cagctttcac atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa
    13801 atccactact ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt
    13861 cgacggtcaa gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga
    13921 tgttcagatg atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga
    13981 tatcattgcc gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa
    14041 aaaagtcgat gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc
    14101 tcatgaattt tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa
    14161 accgcgtttt atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca
    14221 agtggtcaat ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc
    14281 acatgatttg tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt
    14341 gttattagag atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga
    14401 aagtttgtta tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat
    14461 caaatatcaa cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga
    14521 acattttgtc tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca
    14581 aaaggaatct cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc
    14641 cgtatgtatc cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga
    14701 tttacctata accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc
    14761 aatttttcgc tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac
    14821 agtcttgctt tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag
    14881 ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc
    14941 aacaaccaaa cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa
    15001 gaactaatca agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt
    15061 tctgctcgat ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa
    15121 aaagaatctt tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca
    15181 tctcgacctc tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat
    15241 gcacagcgga aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat
    15301 caggcaagca attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag
    15361 aaccagcgag aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac
    15421 ttatttgtag aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg
    15481 cataaattaa taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg
    15541 atcaataccg acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa
    15601 atgggcagac attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg
    15661 gttagccttg ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag
    15721 aaaagctcac taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt
    15781 tttctgctat tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca
    15841 aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc
    15901 ttttgctcac aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg
    15961 ccgattccta caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa
    16021 ttggaatgat cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct
    16081 tccgtaatcg tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta
    16141 ttgactacac ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct
    16201 ttgccagaag actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa
    16261 tggatcttgt tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg
    16321 gcgattagcg gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc
    16381 ggatctcgat taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc
    16441 acaccagttt cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg
    16501 acaaagccac caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac
    16561 aaaactttca gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa
    16621 gagggcttgt catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca
    16681 cttgctttat gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga
    16741 ccacctccat tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac
    16801 caaggattta cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg
    16861 agattgaata aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg
    16921 gcaaagaagc cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct
    16981 gtcataaaac tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa
    17041 tagctgaata ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt
    17101 ttcttctgca tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg
    17161 tgaggggaga aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt
    17221 tattcaacct attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca
    17281 gtctttttca ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga
    17341 aacgaattcg catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt
    17401 atatcactac gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta
    17461 cgattgcatg tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc
    17521 tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa
    17581 aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg
    17641 atatcagtta tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa
    17701 aaatatttga tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt
    17761 actctgtttc tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg
    17821 acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag
    17881 aagaatcata cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag
    17941 aacatcccga attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca
    18001 aaccaggatt agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca
    18061 agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg
    18121 cactaggata tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc
    18181 agatggtcgg taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg
    18241 cccttgattt gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg
    18301 gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg
    18361 gtttagatgc actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct
    18421 ttacgccaga agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact
    18481 tattgaatcg aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg
    18541 cctcacttgt cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac
    18601 gttaccatga tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc
    18661 tgatttctcg tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg
    18721 aagaaaagaa agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg
    18781 cagcgatatt acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag
    18841 gattggactc tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa
    18901 caaaagtagt tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg
    18961 cttttattca agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg
    19021 atccagaaga aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg
    19081 aaaaagtaga actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata
    19141 agttgctgca attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa
    19201 tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa
    19261 atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag
    19321 caccagatgg ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa
    19381 tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat
    19441 ttgagaagca attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa
    19501 acagagaact atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt
    19561 aatttagaat aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt
    19621 cgggcagatt ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt
    19681 ttgaatgcaa tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa
    19741 ttataagttc tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat
    19801 atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa
    19861 atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag
    19921 agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc
    19981 ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga
    20041 gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa
    20101 aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc
    20161 gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac
    20221 caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat
    20281 tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc
    20341 aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc
    20401 gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg
    20461 ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt
    20521 cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc
    20581 agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa
    20641 ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt
    20701 gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca
    20761 ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa
    20821 ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga
    20881 aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt
    20941 gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat
    21001 tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg
    21061 acaaaagtcg gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct
    21121 gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc
    21181 gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg
    21241 tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa
    21301 aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg
    21361 atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca
    21421 gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg
    21481 atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct
    21541 tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag
    21601 aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg
    21661 agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt
    21721 ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca
    21781 tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt
    21841 ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg
    21901 ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca
    21961 acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa
    22021 taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa
    22081 gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt taaatgctga
    22141 acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa
    22201 aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga
    22261 ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa
    22321 agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag cattagggga
    22381 aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg
    22441 cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc
    22501 acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat
    22561 gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat
    22621 ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa
    22681 tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt
    22741 tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact
    22801 tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt
    22861 tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag
    22921 acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg
    22981 caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg
    23041 tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc
    23101 ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc
    23161 ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc
    23221 cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt
    23281 taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg
    23341 gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag
    23401 aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg
    23461 acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta
    23521 ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata
    23581 gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta
    23641 gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg
    23701 gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc
    23761 gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg
    23821 aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt
    23881 gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca
    23941 ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga agccagttta
    24001 gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg
    24061 aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga
    24121 ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg
    24181 tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc
    24241 gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg
    24301 atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct
    24361 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg
    24421 cttgttaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg
    24481 tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt
    24541 tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca
    24601 caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact
    24661 cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga
    24721 gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa
    24781 aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca
    24841 atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca
    24901 ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca
    24961 tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg
    25021 tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga
    25081 cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat
    25141 tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca
    25201 tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc
    25261 cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt
    25321 gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac
    25381 gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg
    25441 ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct
    25501 ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc
    25561 aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa
    25621 atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt
    25681 ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc
    25741 ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt
    25801 tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag
    25861 cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg
    25921 agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt
    25981 accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc
    26041 ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc
    26101 aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat
    26161 taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc
    26221 accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc
    26281 attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc
    26341 catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat
    26401 atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc
    26461 agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg
    26521 accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata
    26581 attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg
    26641 ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta
    26701 acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag
    26761 ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac
    26821 aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc
    26881 tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc
    26941 taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa
    27001 atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga
    27061 tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc
    27121 acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga
    27181 ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat
    27241 agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc
    27301 gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat
    27361 tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta
    27421 tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc
    27481 taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc
    27541 attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc
    27601 ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt
    27661 cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag
    27721 tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt
    27781 tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat
    27841 ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg
    27901 ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc
    27961 ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa
    28021 aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc
    28081 cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc
    28141 ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg
    28201 taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga
    28261 tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa
    28321 ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta
    28381 tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga
    28441 gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta
    28501 gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca
    28561 tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta
    28621 ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt
    28681 tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa
    28741 cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg
    28801 gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa
    28861 gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag
    28921 gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga
    28981 ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa
    29041 aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc
    29101 ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa
    29161 cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga
    29221 tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg
    29281 attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc
    29341 tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt
    29401 ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac
    29461 gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg
    29521 gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca
    29581 taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg
    29641 gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt
    29701 aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct
    29761 tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc
    29821 ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa
    29881 ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct
    29941 cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac
    30001 cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc
    30061 ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca
    30121 aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg
    30181 gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt
    30241 tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg
    30301 cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt
    30361 gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt
    30421 gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac
    30481 cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg
    30541 ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag
    30601 aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag
    30661 aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca
    30721 cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa
    30781 caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt
    30841 gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga
    30901 cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat
    30961 aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg
    31021 caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat
    31081 ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa
    31141 tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa
    31201 aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca
    31261 ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg
    31321 ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac
    31381 gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac
    31441 aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc
    31501 ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt
    31561 cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt
    31621 ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt ccacaattga
    31681 aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt
    31741 ttcataaatg aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta
    31801 aaaaaaagaa aatgagaaaa catgccagtc tcttcattca taggaacgtc tattttactt
    31861 aatttttcta taaatgaatt ctgcataaaa aatatatcaa gtaaatgttt atagatgaaa
    31921 ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa
    31981 aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa
    32041 tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat
    32101 ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca
    32161 aaagctactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg
    32221 tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa
    32281 aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc
    32341 attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac
    32401 aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc
    32461 aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa
    32521 cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa
    32581 gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc
    32641 gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa
    32701 actggaagga actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc
    32761 tataataaga tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt
    32821 ttctatgatg gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag
    32881 aaccagacag cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta
    32941 gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat
    33001 gagcaactac tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt
    33061 gaaaaacaat tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag
    33121 gaacagcctt ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc
    33181 agtttgaaag aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta
    33241 ccacttttga ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat
    33301 cagtattgtc tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt
    33361 gcacaatatg cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga
    33421 gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc
    33481 ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat
    33541 cttgatcaag gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca
    33601 cgtctctttt ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga
    33661 agaataccta tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta
    33721 gaactgattt actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc
    33781 agtggaccag ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg
    33841 aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa
    33901 atccatgtca cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat
    33961 acagcacagc aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag
    34021 caacagcccg aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag
    34081 aaaaataact gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta
    34141 tttgattttt tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg
    34201 acacaatcta tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat
    34261 ggaaacagtg tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta
    34321 ccggaagaac atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct
    34381 tatccatcta gttatcacta tgtccaacga attctggaat taagtaagat caaattagat
    34441 attgaagaca caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg
    34501 tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc
    34561 agtatggcaa atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg
    34621 ccactagatg tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc
    34681 gatgtttcaa atggattgat catattagta gacatgggat ctctgaaaga aatccatcag
    34741 ttttttaaaa aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta
    34801 gcaatcacag taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag
    34861 actgttcgac agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa
    34921 gcactcatta ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta
    34981 gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta
    35041 aaagataaga aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc
    35101 accacagatc ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg
    35161 gaaaagatgt ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa
    35221 atattcaatc agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact
    35281 attctagata cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg
    35341 cagttgaatc gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt
    35401 ctcattgaac gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg
    35461 aagcagtgtc aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag
    35521 cattatagtg tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa
    35581 gatacagagt ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa
    35641 ttttggcatg caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg
    35701 atagagaaat tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag
    35761 agctgatcta tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt
    35821 ttgatttagc ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg
    35881 ttctgacgga tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg
    35941 atcatgtcta tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt
    36001 tggacgctga agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa
    36061 tgatccagtt ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg
    36121 aagaaaaatg attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt
    36181 ttcatggaca caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac
    36241 gaatgagata cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat
    36301 caaatcaatt gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt
    36361 attcatcgta gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat
    36421 caaaaccatc aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc
    36481 ggtaaatatt ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga
    36541 agtagaaatc cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga
    36601 aaggaagaac ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac
    36661 aatcagaata tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta
    36721 caggaatcgt gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag
    36781 catttatcgg atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga
    36841 ttttagggac ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag
    36901 gtttgccgat tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc
    36961 caattatgaa ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc
    37021 atatgcactt gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt
    37081 cttatatggt gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc
    37141 aaaaggggtt gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc
    37201 gtttgctgat caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt
    37261 acttagagat tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag
    37321 tcaatattat gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag
    37381 atgaagaatt ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt
    37441 tggcgttctt ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat
    37501 gtttatgcaa tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca
    37561 gctttgaaac ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta
    37621 ggaatcacgg ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg
    37681 atcgatagta tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt
    37741 tggggaactt tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat
    37801 attttaggcc ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat
    37861 ctagcaacag gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac
    37921 ggtatgatgg aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca
    37981 atgactgcca cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct
    38041 gtgactgttc aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt
    38101 ggcatagtgt tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta
    38161 gctttctttg gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat
    38221 gtttacgatg caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa
    38281 gaatgatttt tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac
    38341 gggttcatct tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct
    38401 tactgtcgat attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat
    38461 cgatactgtg attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa
    38521 aatgatacaa aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact
    38581 tgctttagaa gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt
    38641 tacaaaaggc tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat
    38701 aagtaaaaat aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat
    38761 aatcaaccat ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag
    38821 tttatttcgt ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc
    38881 taaagaagca gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt
    38941 agaagcttat atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat
    39001 tgaaggggaa agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta
    39061 cgttggagag actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt
    39121 agcaatagaa tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt
    39181 cgcttataaa accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt
    39241 tgatacagtc ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga
    39301 agaacaaatc aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat
    39361 cgataaactt tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta
    39421 tgcttcctcg atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt
    39481 acagcatgct gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt
    39541 tattttgtcc tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa
    39601 gaaagtagga gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct
    39661 atgcgatttt gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc
    39721 agcagatcga ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca
    39781 gctcgaacgt tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg
    39841 cttatcattt gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa
    39901 tcaatgggga gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc
    39961 atcgaaatct atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac
    40021 aaccccagtc acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc
    40081 taaacttttg ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt
    40141 agaaggtatc agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt
    40201 gacacaacgc atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg
    40261 ctactatcgt cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga
    40321 gagcagcgat aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa
    40381 cacatttttt gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc
    40441 caaccatttt ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt
    40501 ccaagtacaa cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa
    40561 caaaagttcc tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat
    40621 ttagaaatgt tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc
    40681 ctaacctcct aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca
    40741 agcaaagcat aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt
    40801 tcaagtaaaa acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa
    40861 ctttaagaaa tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg
    40921 aaacaaaaaa tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg
    40981 gcagaaaacg ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc
    41041 aataagtatc atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc
    41101 ggatataaag aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca
    41161 acaaaagcga ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc
    41221 attatacatg caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg
    41281 gctgaagaaa ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat
    41341 gctgaggaag tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa
    41401 gcagcagcct atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat
    41461 gatgtgattg gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc
    41521 gctatccatg gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg
    41581 gcagaaatca gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg
    41641 agtggtgtgg gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc
    41701 ggtaacttgt taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc
    41761 gttgagggtg tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag
    41821 atgccaatta ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca
    41881 gcaaaagaaa tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata
    41941 gatgatcaat agttaatc
//
LOCUS       DACECS010000020        37552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.020, whole genome shotgun sequence.
ACCESSION   DACECS010000020 DACECS010000000
VERSION     DACECS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.020"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IWO31_001133"
                     /pseudo
     CDS             75..470
                     /locus_tag="IWO31_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IWO31_001134"
     CDS             790..1104
                     /locus_tag="IWO31_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0301565.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IWO31_001135"
     CDS             1208..2386
                     /locus_tag="IWO31_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0301566.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IWO31_001136"
     CDS             2789..3082
                     /locus_tag="IWO31_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0301567.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            3925..6252
                     /locus_tag="IWO31_001137"
     CDS             3925..6252
                     /locus_tag="IWO31_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0301568.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="IWO31_001138"
     CDS             6378..6707
                     /locus_tag="IWO31_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0301569.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="IWO31_001139"
     CDS             6747..7751
                     /locus_tag="IWO31_001139"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0301570.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="IWO31_001140"
     CDS             7748..8194
                     /locus_tag="IWO31_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0301571.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="IWO31_001141"
     CDS             8344..9159
                     /locus_tag="IWO31_001141"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0301572.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="IWO31_001142"
     CDS             9365..10537
                     /locus_tag="IWO31_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0301573.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="IWO31_001143"
     CDS             complement(10699..11193)
                     /locus_tag="IWO31_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301574.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="IWO31_001144"
     CDS             11776..12198
                     /locus_tag="IWO31_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0301575.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="IWO31_001145"
     CDS             complement(12364..13557)
                     /locus_tag="IWO31_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301576.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="IWO31_001146"
     CDS             13781..14158
                     /locus_tag="IWO31_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0301577.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="IWO31_001147"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="IWO31_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14618..15061
                     /locus_tag="IWO31_001148"
     CDS             14618..15061
                     /locus_tag="IWO31_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0301578.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="IWO31_001149"
     CDS             15322..16137
                     /locus_tag="IWO31_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0301579.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="IWO31_001150"
     CDS             16147..16809
                     /locus_tag="IWO31_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0301580.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="IWO31_001151"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="IWO31_001151"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0301581.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="IWO31_001152"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="IWO31_001152"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0301582.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="IWO31_001153"
     CDS             19534..20031
                     /locus_tag="IWO31_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0301583.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="IWO31_001154"
     CDS             20216..20476
                     /locus_tag="IWO31_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301584.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="IWO31_001155"
     CDS             complement(20838..21077)
                     /locus_tag="IWO31_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301585.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="IWO31_001156"
     CDS             21363..21677
                     /locus_tag="IWO31_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301586.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="IWO31_001157"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="IWO31_001157"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="IWO31_001158"
     CDS             complement(22648..23421)
                     /locus_tag="IWO31_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0301587.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="IWO31_001159"
     CDS             23565..23915
                     /locus_tag="IWO31_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0301588.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="IWO31_001160"
     CDS             23896..24612
                     /locus_tag="IWO31_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301589.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="IWO31_001161"
     CDS             24612..26060
                     /locus_tag="IWO31_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0301590.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="IWO31_001162"
     CDS             complement(26130..26639)
                     /locus_tag="IWO31_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301591.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="IWO31_001163"
     CDS             complement(26629..27354)
                     /locus_tag="IWO31_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301592.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="IWO31_001164"
     CDS             complement(27577..29697)
                     /locus_tag="IWO31_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0301593.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="IWO31_001165"
     CDS             29927..31105
                     /locus_tag="IWO31_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0301594.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="IWO31_001166"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="IWO31_001166"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0301595.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="IWO31_001167"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="IWO31_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0301596.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="IWO31_001168"
     CDS             complement(32458..33711)
                     /locus_tag="IWO31_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0301597.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="IWO31_001169"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="IWO31_001169"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0301598.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="IWO31_001170"
     CDS             35290..36636
                     /locus_tag="IWO31_001170"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0301599.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="IWO31_001171"
     CDS             complement(36944..37330)
                     /locus_tag="IWO31_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0301600.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(37379..>37552)
                     /locus_tag="IWO31_001172"
     CDS             complement(37379..>37552)
                     /locus_tag="IWO31_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0301601.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT        12546 a         6489 c         7434 g        11083 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatgggc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttattatac ccgtggtgct agttaatttt
    37381 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
    37441 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
    37501 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact gt
//
LOCUS       DACECS010000021        34330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.021, whole genome shotgun sequence.
ACCESSION   DACECS010000021 DACECS010000000
VERSION     DACECS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.021"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWO31_001173"
     CDS             202..1077
                     /locus_tag="IWO31_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301602.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWO31_001174"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWO31_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWO31_001175"
     CDS             complement(1642..2973)
                     /locus_tag="IWO31_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0301603.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWO31_001176"
     CDS             complement(3122..3835)
                     /locus_tag="IWO31_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0301604.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWO31_001177"
     CDS             4010..5434
                     /locus_tag="IWO31_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0301605.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWO31_001178"
     CDS             5733..6440
                     /locus_tag="IWO31_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0301606.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWO31_001179"
     CDS             6427..6750
                     /locus_tag="IWO31_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0301607.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWO31_001180"
     CDS             complement(6839..7876)
                     /locus_tag="IWO31_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0301608.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWO31_001181"
     CDS             complement(8197..9180)
                     /locus_tag="IWO31_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0301609.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IWO31_001182"
     CDS             complement(9595..10365)
                     /locus_tag="IWO31_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301610.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IWO31_001183"
     CDS             complement(10358..11293)
                     /locus_tag="IWO31_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301611.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IWO31_001184"
     CDS             complement(11609..13258)
                     /locus_tag="IWO31_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0301612.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IWO31_001185"
     CDS             complement(13375..15978)
                     /locus_tag="IWO31_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0301613.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IWO31_001186"
     CDS             complement(15990..17039)
                     /locus_tag="IWO31_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0301614.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IWO31_001187"
     CDS             complement(17065..17379)
                     /locus_tag="IWO31_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0301615.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IWO31_001188"
     CDS             complement(17395..17841)
                     /locus_tag="IWO31_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0301616.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="IWO31_001189"
     CDS             complement(17842..19818)
                     /locus_tag="IWO31_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0301617.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="IWO31_001190"
     CDS             complement(19976..20872)
                     /locus_tag="IWO31_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0301618.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="IWO31_001191"
     CDS             complement(20865..21545)
                     /locus_tag="IWO31_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301619.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="IWO31_001192"
     CDS             complement(21546..22328)
                     /locus_tag="IWO31_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0301620.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="IWO31_001193"
     CDS             complement(22321..23238)
                     /locus_tag="IWO31_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301621.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="IWO31_001194"
     CDS             complement(23402..23725)
                     /locus_tag="IWO31_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301622.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="IWO31_001195"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="IWO31_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0301623.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="IWO31_001196"
     CDS             27853..28167
                     /locus_tag="IWO31_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0301624.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="IWO31_001197"
     CDS             28180..28554
                     /locus_tag="IWO31_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0301625.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="IWO31_001198"
     CDS             28564..28899
                     /locus_tag="IWO31_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0301626.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="IWO31_001199"
     CDS             28982..30322
                     /locus_tag="IWO31_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0301627.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="IWO31_001200"
     CDS             30400..31074
                     /locus_tag="IWO31_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0301628.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31307..31741
                     /gene="dcm"
                     /locus_tag="IWO31_001201"
     CDS             31307..31741
                     /gene="dcm"
                     /locus_tag="IWO31_001201"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0301629.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            31742..32449
                     /locus_tag="IWO31_001202"
     CDS             31742..32449
                     /locus_tag="IWO31_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0301630.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            32439..32729
                     /locus_tag="IWO31_001203"
     CDS             32439..32729
                     /locus_tag="IWO31_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301631.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="IWO31_001204"
     CDS             32988..34172
                     /locus_tag="IWO31_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0301632.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34187..34306
                     /locus_tag="IWO31_001205"
     CDS             34187..34306
                     /locus_tag="IWO31_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0301633.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DACECS010000022        33736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.022, whole genome shotgun sequence.
ACCESSION   DACECS010000022 DACECS010000000
VERSION     DACECS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.022"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWO31_001206"
     CDS             214..498
                     /locus_tag="IWO31_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0301634.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWO31_001207"
     CDS             complement(779..1198)
                     /locus_tag="IWO31_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0301635.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="IWO31_001208"
     CDS             complement(1200..1784)
                     /locus_tag="IWO31_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0301636.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWO31_001209"
     CDS             complement(1831..3306)
                     /locus_tag="IWO31_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0301637.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWO31_001210"
     CDS             3487..3798
                     /locus_tag="IWO31_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301638.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWO31_001211"
     CDS             complement(3913..6249)
                     /locus_tag="IWO31_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301639.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWO31_001212"
     CDS             6391..7059
                     /locus_tag="IWO31_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301640.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWO31_001213"
     CDS             7062..8105
                     /locus_tag="IWO31_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0301641.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWO31_001214"
     CDS             complement(8342..9223)
                     /locus_tag="IWO31_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0301642.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWO31_001215"
     CDS             9382..10110
                     /locus_tag="IWO31_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0301643.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWO31_001216"
     CDS             10266..10583
                     /locus_tag="IWO31_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0301644.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWO31_001217"
     CDS             10576..11184
                     /locus_tag="IWO31_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0301645.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWO31_001218"
     CDS             11200..12030
                     /locus_tag="IWO31_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0301646.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IWO31_001219"
     CDS             12168..12680
                     /locus_tag="IWO31_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0301647.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IWO31_001220"
     CDS             12706..13578
                     /locus_tag="IWO31_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0301648.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IWO31_001221"
     CDS             13754..14116
                     /locus_tag="IWO31_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0301649.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IWO31_001222"
     CDS             14125..14877
                     /locus_tag="IWO31_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301650.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IWO31_001223"
     CDS             14891..16693
                     /locus_tag="IWO31_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301651.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IWO31_001224"
     CDS             16970..18010
                     /locus_tag="IWO31_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0301652.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="IWO31_001225"
     CDS             complement(18062..20206)
                     /locus_tag="IWO31_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0301653.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="IWO31_001226"
     CDS             complement(20563..21936)
                     /locus_tag="IWO31_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0301654.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWO31_001227"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWO31_001227"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0301655.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWO31_001228"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWO31_001228"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0301656.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="IWO31_001229"
     CDS             complement(23384..24397)
                     /locus_tag="IWO31_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0301657.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="IWO31_001230"
     CDS             complement(24478..25077)
                     /locus_tag="IWO31_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0301658.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="IWO31_001231"
     CDS             25263..25964
                     /locus_tag="IWO31_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301659.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="IWO31_001232"
     CDS             25974..28685
                     /locus_tag="IWO31_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0301660.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="IWO31_001233"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="IWO31_001233"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0301661.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="IWO31_001234"
     CDS             complement(30770..31951)
                     /locus_tag="IWO31_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0301662.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="IWO31_001235"
     CDS             32203..33726
                     /locus_tag="IWO31_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0301663.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10690 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaa
//
LOCUS       DACECS010000023        31835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.023, whole genome shotgun sequence.
ACCESSION   DACECS010000023 DACECS010000000
VERSION     DACECS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.023"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..2305)
                     /locus_tag="IWO31_001236"
     CDS             complement(152..2305)
                     /locus_tag="IWO31_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0301664.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="IWO31_001237"
     CDS             complement(2599..3447)
                     /locus_tag="IWO31_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0301665.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IWO31_001238"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IWO31_001238"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0301666.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="IWO31_001239"
     CDS             complement(5948..6127)
                     /locus_tag="IWO31_001239"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0301667.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IWO31_001240"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IWO31_001240"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0301668.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="IWO31_001241"
     CDS             8275..10554
                     /locus_tag="IWO31_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0301669.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="IWO31_001242"
     CDS             complement(10647..11294)
                     /locus_tag="IWO31_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301670.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="IWO31_001243"
     CDS             complement(11473..12192)
                     /locus_tag="IWO31_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0301671.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="IWO31_001244"
     CDS             complement(12323..13474)
                     /locus_tag="IWO31_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0301672.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="IWO31_001245"
     CDS             complement(13691..14431)
                     /locus_tag="IWO31_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0301673.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IWO31_001246"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IWO31_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0301674.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /locus_tag="IWO31_001247"
     CDS             complement(14815..15408)
                     /locus_tag="IWO31_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0301675.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16039)
                     /locus_tag="IWO31_001248"
     CDS             complement(15392..16039)
                     /locus_tag="IWO31_001248"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0301676.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IWO31_001249"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IWO31_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0301677.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IWO31_001250"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IWO31_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0301678.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="IWO31_001251"
     CDS             complement(17485..18012)
                     /locus_tag="IWO31_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0301679.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="IWO31_001252"
     CDS             complement(18192..18977)
                     /locus_tag="IWO31_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0301680.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /locus_tag="IWO31_001253"
     CDS             complement(18970..19839)
                     /locus_tag="IWO31_001253"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0301681.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="IWO31_001254"
     CDS             complement(19840..21219)
                     /locus_tag="IWO31_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0301682.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IWO31_001255"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IWO31_001255"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0301683.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IWO31_001256"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IWO31_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0301684.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IWO31_001257"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IWO31_001257"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0301685.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IWO31_001258"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IWO31_001258"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0301686.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IWO31_001259"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IWO31_001259"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0301687.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="IWO31_001260"
     CDS             complement(25177..25401)
                     /locus_tag="IWO31_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0301688.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="IWO31_001261"
     CDS             complement(25454..26416)
                     /locus_tag="IWO31_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0301689.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="IWO31_001262"
     CDS             complement(26426..26866)
                     /locus_tag="IWO31_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0301690.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27172..28137
                     /locus_tag="IWO31_001263"
     CDS             27172..28137
                     /locus_tag="IWO31_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0301691.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28194..28424)
                     /locus_tag="IWO31_001264"
     CDS             complement(28194..28424)
                     /locus_tag="IWO31_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0301692.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28510..29397)
                     /locus_tag="IWO31_001265"
     CDS             complement(28510..29397)
                     /locus_tag="IWO31_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0301693.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29486..30709)
                     /locus_tag="IWO31_001266"
     CDS             complement(29486..30709)
                     /locus_tag="IWO31_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0301694.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30726..31805)
                     /locus_tag="IWO31_001267"
     CDS             complement(30726..31805)
                     /locus_tag="IWO31_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0301695.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8793 a         6970 c         5488 g        10584 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    26941 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    27001 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    27061 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    27121 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    27181 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    27241 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    27301 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    27361 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    27421 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    27481 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    27541 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    27601 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    27661 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    27721 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    27781 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    27841 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    27901 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    27961 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    28021 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    28081 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    28141 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    28201 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    28261 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    28321 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    28381 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    28441 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    28501 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    28561 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    28621 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    28681 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    28741 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    28801 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    28861 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    28921 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    28981 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    29041 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    29101 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    29161 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    29221 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    29281 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    29341 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    29401 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    29461 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    29521 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    29581 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    29641 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    29701 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    29761 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    29821 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    29881 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    29941 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    30001 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    30061 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    30121 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    30181 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    30241 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    30301 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    30361 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    30421 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    30481 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    30541 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    30601 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    30661 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    30721 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    30781 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    30841 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    30901 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    30961 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    31021 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    31081 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    31141 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    31201 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    31261 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    31321 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    31381 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    31441 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    31501 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    31561 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    31621 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    31681 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    31741 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    31801 aacatagcct acaaactgtc actcaaactt aagcc
//
LOCUS       DACECS010000024        31597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.024, whole genome shotgun sequence.
ACCESSION   DACECS010000024 DACECS010000000
VERSION     DACECS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.024"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..543)
                     /locus_tag="IWO31_001268"
     CDS             complement(76..543)
                     /locus_tag="IWO31_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0301696.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IWO31_001269"
     CDS             complement(663..2201)
                     /locus_tag="IWO31_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0301697.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IWO31_001270"
     CDS             2281..2967
                     /locus_tag="IWO31_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0301698.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IWO31_001271"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IWO31_001271"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0301699.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IWO31_001272"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IWO31_001272"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0301700.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IWO31_001273"
     CDS             complement(4453..5583)
                     /locus_tag="IWO31_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0301701.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IWO31_001274"
     CDS             complement(5583..7766)
                     /locus_tag="IWO31_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0301702.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IWO31_001275"
     CDS             complement(7763..8788)
                     /locus_tag="IWO31_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0301703.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IWO31_001276"
     CDS             complement(8833..9660)
                     /locus_tag="IWO31_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0301704.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IWO31_001277"
     CDS             complement(9657..10724)
                     /locus_tag="IWO31_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0301705.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IWO31_001278"
     CDS             complement(10750..11586)
                     /locus_tag="IWO31_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0301706.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IWO31_001279"
     CDS             complement(11583..12452)
                     /locus_tag="IWO31_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0301707.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IWO31_001280"
     CDS             complement(12522..13805)
                     /locus_tag="IWO31_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0301708.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IWO31_001281"
     CDS             complement(13819..15504)
                     /locus_tag="IWO31_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0301709.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IWO31_001282"
     CDS             15779..16804
                     /locus_tag="IWO31_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0301710.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IWO31_001283"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IWO31_001283"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0301711.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IWO31_001284"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IWO31_001284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0301712.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IWO31_001285"
     CDS             25476..26444
                     /locus_tag="IWO31_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0301713.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IWO31_001286"
     CDS             26462..27367
                     /locus_tag="IWO31_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301714.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IWO31_001287"
     CDS             27364..28170
                     /locus_tag="IWO31_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301715.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IWO31_001288"
     CDS             28327..29295
                     /locus_tag="IWO31_001288"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0301716.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IWO31_001289"
     CDS             complement(29612..30136)
                     /locus_tag="IWO31_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0301717.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31597
                     /gene="scm"
                     /locus_tag="IWO31_001290"
     CDS             30423..>31597
                     /gene="scm"
                     /locus_tag="IWO31_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0301718.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     "
BASE COUNT         8874 a         6830 c         5947 g         9946 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    31501 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    31561 cagatactac ctctaccaca agtgatagca cggacac
//
LOCUS       DACECS010000025        29624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.025, whole genome shotgun sequence.
ACCESSION   DACECS010000025 DACECS010000000
VERSION     DACECS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.025"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(72..542)
                     /locus_tag="IWO31_001291"
     CDS             complement(72..542)
                     /locus_tag="IWO31_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0301719.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IWO31_001292"
     CDS             872..2512
                     /locus_tag="IWO31_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0301720.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IWO31_001293"
     CDS             2666..3988
                     /locus_tag="IWO31_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0301721.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IWO31_001294"
     CDS             complement(4056..4490)
                     /locus_tag="IWO31_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301722.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5392)
                     /locus_tag="IWO31_001295"
                     /pseudo
     CDS             complement(4555..5392)
                     /locus_tag="IWO31_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(5961..6266)
                     /locus_tag="IWO31_001296"
     CDS             complement(5961..6266)
                     /locus_tag="IWO31_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0301723.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6291..6722)
                     /gene="ruvX"
                     /locus_tag="IWO31_001297"
     CDS             complement(6291..6722)
                     /gene="ruvX"
                     /locus_tag="IWO31_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0301724.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6719..6985)
                     /locus_tag="IWO31_001298"
     CDS             complement(6719..6985)
                     /locus_tag="IWO31_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0301725.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7240..7965)
                     /locus_tag="IWO31_001299"
     CDS             complement(7240..7965)
                     /locus_tag="IWO31_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0301726.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8326..8396)
                     /locus_tag="IWO31_001300"
     tRNA            complement(8326..8396)
                     /locus_tag="IWO31_001300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8362..8364),aa:Gly,seq:ccc)
     gene            8513..9265
                     /locus_tag="IWO31_001301"
     CDS             8513..9265
                     /locus_tag="IWO31_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0301727.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9281..9760
                     /locus_tag="IWO31_001302"
     CDS             9281..9760
                     /locus_tag="IWO31_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0301728.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10011..11591)
                     /locus_tag="IWO31_001303"
     CDS             complement(10011..11591)
                     /locus_tag="IWO31_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0301729.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11584..12327)
                     /locus_tag="IWO31_001304"
     CDS             complement(11584..12327)
                     /locus_tag="IWO31_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301730.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12503..13279)
                     /locus_tag="IWO31_001305"
     CDS             complement(12503..13279)
                     /locus_tag="IWO31_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0301731.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13527..14363)
                     /locus_tag="IWO31_001306"
     CDS             complement(13527..14363)
                     /locus_tag="IWO31_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0301732.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14374..15303)
                     /locus_tag="IWO31_001307"
     CDS             complement(14374..15303)
                     /locus_tag="IWO31_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0301733.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15495..15848
                     /locus_tag="IWO31_001308"
     CDS             15495..15848
                     /locus_tag="IWO31_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0301734.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(15922..16578)
                     /locus_tag="IWO31_001309"
     CDS             complement(15922..16578)
                     /locus_tag="IWO31_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0301735.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16804..18738
                     /locus_tag="IWO31_001310"
     CDS             16804..18738
                     /locus_tag="IWO31_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0301736.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18824..20047)
                     /locus_tag="IWO31_001311"
     CDS             complement(18824..20047)
                     /locus_tag="IWO31_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0301737.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20041..21939)
                     /locus_tag="IWO31_001312"
     CDS             complement(20041..21939)
                     /locus_tag="IWO31_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0301738.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(21933..23051)
                     /locus_tag="IWO31_001313"
     CDS             complement(21933..23051)
                     /locus_tag="IWO31_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0301739.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23134..23967)
                     /locus_tag="IWO31_001314"
     CDS             complement(23134..23967)
                     /locus_tag="IWO31_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0301740.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(23960..24877)
                     /locus_tag="IWO31_001315"
     CDS             complement(23960..24877)
                     /locus_tag="IWO31_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0301741.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25104..26228)
                     /locus_tag="IWO31_001316"
     CDS             complement(25104..26228)
                     /locus_tag="IWO31_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0301742.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26411..27319
                     /locus_tag="IWO31_001317"
     CDS             26411..27319
                     /locus_tag="IWO31_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0301743.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27460..29073
                     /locus_tag="IWO31_001318"
     CDS             27460..29073
                     /locus_tag="IWO31_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0301744.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9158 a         5803 c         5054 g         9609 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     4981 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5041 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5101 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5161 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5221 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5281 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5341 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5401 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5461 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5521 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5581 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5641 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5701 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
     5761 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
     5821 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
     5881 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
     5941 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgtatt gaatacttct
     6001 tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct
     6061 tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagca
     6121 ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagtaggatt
     6181 tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcatgg
     6241 tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta ttaaaataat
     6301 tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa tttatcgatg
     6361 actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga
     6421 acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc tttgatcttt
     6481 tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat atttttaggt
     6541 aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa ccgatcaaag
     6601 ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc tgtccagcca
     6661 aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc cattactctc
     6721 ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg atctcatcgc
     6781 gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag gccgggtctc
     6841 cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc
     6901 gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac
     6961 gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc
     7021 ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa
     7081 cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct gtagctttat
     7141 caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt ttttacacaa
     7201 ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg tttttgttgc
     7261 ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa agacattgaa
     7321 ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg ctaccagttc
     7381 attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt ctttgatata
     7441 tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac gtgtccctgt
     7501 catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta aatggctgcg
     7561 attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg ggtcaatata
     7621 caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat tggcttgtcc
     7681 tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg aaaggctaag
     7741 atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat gttttccatc
     7801 atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa tagttgtaat
     7861 ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc ctgcaatcac
     7921 agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta
     7981 ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag aaagaaatag
     8041 aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa gagacggatt
     8101 cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt caaaaatttt
     8161 cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg cgcaaaagct
     8221 tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt ttgcttggct
     8281 gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg gtgacgggaa
     8341 tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac acccgcaata
     8401 caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt tacgcttctg
     8461 gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg ctatgacggg
     8521 aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca tgatggaaag
     8581 caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag cttctggaaa
     8641 tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg atcgtacctc
     8701 gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt
     8761 aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac tttactgcga
     8821 actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc
     8881 ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg atttacctgt
     8941 gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga aattttttag
     9001 aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg ctatctacag
     9061 cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg tgatgccttt
     9121 agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac attatgtctc
     9181 aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt ttgcgattgc
     9241 ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga tatttattca
     9301 gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg tacctaggcg
     9361 ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact ggatctgtgt
     9421 tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag tagcattcat
     9481 cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata ttcccgggat
     9541 ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg ttactgaatc
     9601 ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag caatcgcttt
     9661 aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag taaagcgtga
     9721 cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta acaaagaaaa
     9781 ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa cattgcgaca
     9841 agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag
     9901 cttctcacat ttttacataa aattgactta atactgagaa actgactttt gaacaccctc
     9961 tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct ttattcaaat
    10021 tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat tagaactaaa
    10081 ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat tccgtataag
    10141 acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa acccaaactt
    10201 cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag caaccgataa
    10261 tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc aaatagcaag
    10321 ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc gatcccacca
    10381 gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata ttggttcata
    10441 gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat aagattgctg
    10501 ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag gaaaacaaca
    10561 ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc aaaagcaaga
    10621 ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa attaggttct
    10681 tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt ctttttatgg
    10741 gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag ctttggtact
    10801 atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat tccccaaccc
    10861 aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa taatatcgaa
    10921 atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt catccacatg
    10981 attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac tgtttttcta
    11041 tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa
    11101 agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag caaaatccct
    11161 agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac aaaaggaatg
    11221 atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga
    11281 ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac tgatatactt
    11341 tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc tggcaattta
    11401 acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa
    11461 agattttgag acaaaagata gatagttaat cgctttccct tttttccttt ctctcttgct
    11521 ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc taataattgt
    11581 cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca aataaatctt
    11641 ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct gataaatcaa
    11701 ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat ccaatacatg
    11761 tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc
    11821 aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa tatctggatc
    11881 tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat
    11941 tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta aacgcttttc
    12001 ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt ctccagccgt
    12061 caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt tgatactcaa
    12121 tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg gttcaataat
    12181 actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta aaaatccaaa
    12241 aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt tcccgtaact
    12301 tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta ttacttttta
    12361 ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag
    12421 aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct tctaacaggt
    12481 aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct tgcataaaat
    12541 tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt gcaaatgtta
    12601 ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata taagctttta
    12661 aatctttaca taaatactca tattcgagtg attttttata ttttttaatt gtgccgttta
    12721 cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct ggcatgctca
    12781 ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa cggacagttg
    12841 tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc aataagtaac
    12901 tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa ccactaggcg
    12961 taatctttcg attaataaca gttattgctt tattcggatg agacgttaga taaacaattt
    13021 tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta taataggcgt
    13081 tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt ttaatgaatt
    13141 caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga cttaataaaa
    13201 ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta tcatccaaaa
    13261 taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc aaaatcattt
    13321 catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac aatcttttcc
    13381 aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat aaatatcaaa
    13441 caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg tcagctgtct
    13501 gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg atttttcgga
    13561 gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt gaatggttga
    13621 taatagtctg attggatggt tccttctatt ttataccatt catcattttt aaccgttact
    13681 ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca atggataatg
    13741 ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta aacaaatcct
    13801 tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag ctccataact
    13861 tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt ttctttcaac
    13921 tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc cggctttaag
    13981 tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa attaaatcct
    14041 ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc cacgattact
    14101 ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa cccatggtca
    14161 tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa atacagctga
    14221 actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg ataatggaca
    14281 ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc gaaataccca
    14341 gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga atccaataac
    14401 gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat atcgtttcat
    14461 catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag ctacaggtgc
    14521 tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg agcaaagaga
    14581 catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg agccgagcgt
    14641 tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc cgccaatgat
    14701 caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat gcccagtttg
    14761 atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat ggacatgatg
    14821 atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt ctttttgaaa
    14881 ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca cccgccagct
    14941 tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga ttggattgat
    15001 gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt ttttaacaaa
    15061 ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc ctaagatcga
    15121 accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac gttctggggt
    15181 caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag gtaatgcttc
    15241 aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa taaaagaaaa
    15301 catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg cttttattca
    15361 aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca aagcacttca
    15421 attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact tctatcataa
    15481 aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac atactgcaac
    15541 agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt cacgctgttc
    15601 gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat ttggatgcat
    15661 ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt gtcctgattt
    15721 ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa aaaatccgtt
    15781 atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa atcttccgaa
    15841 caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt ttgccttcat
    15901 ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat ttgcgtcaga
    15961 atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt ggatcaatac
    16021 atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga ttcctgcttc
    16081 cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa ttgctacttc
    16141 gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg ggataccgtc
    16201 tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac gtatgctatt
    16261 actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac cgatcagcgc
    16321 aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa gatcttctac
    16381 atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc gacggatcgt
    16441 tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt tctttccagc
    16501 attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg tcgcctttgg
    16561 aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc tttgatttct
    16621 ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt gtgaaatttt
    16681 attctttatt catttattta tcatttctgc aaatctcgca gtataatgga atttatggta
    16741 gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag aaaagaggat
    16801 tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga agtcttattt
    16861 gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg cagaaatggc
    16921 gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga tgaaggaaag
    16981 atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg acttgattcc
    17041 aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca aatggaagag
    17101 cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc tgcttatagt
    17161 tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa cggctatggt
    17221 tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc attttatgat
    17281 cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc tcgcatgctt
    17341 ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga tatcgagaca
    17401 ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat tgtttcccat
    17461 gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag aagaaaaatg
    17521 cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca gttagcaagt
    17581 gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga cttcgttgct
    17641 cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa gacattagaa
    17701 aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt tctattcagt
    17761 atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat cggatatgat
    17821 aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc gattgctcta
    17881 gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca ccagatccct
    17941 tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta cgatcaaggt
    18001 caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga gcatcctacc
    18061 actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg cgaagatgta
    18121 gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt agccaagtta
    18181 gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt agatattgac
    18241 aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct ttttgtttcc
    18301 catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc tgaaaatggc
    18361 agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga agaagaagaa
    18421 atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac gaacaaatct
    18481 gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg aaaaattact
    18541 caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga acaagaaatg
    18601 gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca agatttagaa
    18661 gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag cttggaatta
    18721 gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg ttttggtttt
    18781 tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa taagaagacc
    18841 aattactagt cattctcact aaagcttcta aataatagta atcaccccaa atacaaggtg
    18901 tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag ccatcttctt
    18961 cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca gcagactggt
    19021 ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat aatccacaaa
    19081 ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca aagatcaaat
    19141 cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct aatattttgc
    19201 ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa ccgtaaatcc
    19261 cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc gtacttccat
    19321 atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa ccatcttctc
    19381 ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta tatttttgat
    19441 ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa tccatgatca
    19501 ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact cctgccttct
    19561 cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc atttttcgat
    19621 ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca agatcgtgat
    19681 gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct ttttcagctg
    19741 tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt cctgtccaaa
    19801 atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata ggacactcgc
    19861 ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg tgtctgcgac
    19921 aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat tcatttatcc
    19981 atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc ccaccagcct
    20041 ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc cggttgtccc
    20101 atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc gatcttgatg
    20161 agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct ctaatccagg
    20221 aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa tagctgtgga
    20281 ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag cttcactcac
    20341 cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag aacattcaat
    20401 agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt gtacgcgaat
    20461 ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag taggttccca
    20521 tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc gaacgatgaa
    20581 ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg tgtccgctcg
    20641 ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg ctcgcggtat
    20701 cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg tcaatgtatg
    20761 gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat catgttgtat
    20821 gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag gcatttcttt
    20881 agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct tattcagcca
    20941 taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt aggtataacc
    21001 aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc ttatattccg
    21061 attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc acaacatagc
    21121 tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag gcacatttgc
    21181 accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc ggttcttata
    21241 tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat aataatgaaa
    21301 tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc agccattttg
    21361 aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt taactggata
    21421 accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat taccatctgc
    21481 acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt ctcctgtatc
    21541 taattttttc cataataatt gttcatgatg atataacgct aataacattg cgggcatttc
    21601 taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg aaggatctgt
    21661 tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt ggtcaattgc
    21721 gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt tatttccata
    21781 tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa acggctggag
    21841 tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta atagtggtac
    21901 tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc cgactgttct
    21961 aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac attataagtc
    22021 agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc catattccaa
    22081 tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg atacattttt
    22141 gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc gcatactcca
    22201 gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat ataaccagtt
    22261 ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag atagtacgga
    22321 ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt tcctctgctc
    22381 caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt ccctgattct
    22441 gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata cttagctgtt
    22501 gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc cctcgaagcc
    22561 caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg tttatggtca
    22621 ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac cgtagctgca
    22681 tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc aatagccgat
    22741 ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag ggcagattct
    22801 aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt cttgtgaaag
    22861 agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc tctgtccttt
    22921 attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc cattctgtct
    22981 aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca ttccttcgtc
    23041 tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag ttgctcctag
    23101 aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc caatgtcatt
    23161 ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca ctgaagttgc
    23221 taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa ccgtcatatt
    23281 tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta atggaaatag
    23341 atattgattc caacttccca taaagataag taaaccacta gtaataatcg taggtgataa
    23401 aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga ttctggcagc
    23461 ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca ctgaagttgt
    23521 actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta aacgactcat
    23581 aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata gcataactgt
    23641 attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac ccgccattgt
    23701 agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat tccaaataat
    23761 caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca tatccccaaa
    23821 cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag ctacaatcat
    23881 atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt acgtaaaaat
    23941 tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat tgaatagccg
    24001 ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt ccaaaatcaa
    24061 ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa ttttctggtc
    24121 cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca ttgatagtgg
    24181 ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat aaaaagaatt
    24241 gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta gaaatccctt
    24301 gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc atcgtgatga
    24361 tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta attcctagcg
    24421 cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat acggaagcta
    24481 cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat ccttttcctt
    24541 ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc gttattggaa
    24601 ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat ggatctcgga
    24661 agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt ttcataaaac
    24721 taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa ataacaaaag
    24781 gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt ttcttgctgt
    24841 tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc taatattttt
    24901 taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc ttttagatat
    24961 gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa ataagacgta
    25021 gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat tttattcaat
    25081 agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc ttctgtttga
    25141 taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa gcattcttca
    25201 tctgtcagca taaatgtaag aaattgttgt gccacttgtg gatttttggc atctttaggg
    25261 acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt tgaaaaagga
    25321 atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga catattcgta
    25381 gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc agcatcttgt
    25441 gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt tgcagcatgt
    25501 ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga ttgattctgc
    25561 tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc aattccaaaa
    25621 ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca agaactgatt
    25681 tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca aagtgctggg
    25741 gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg caaaatattc
    25801 aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc atcagcacta
    25861 taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc catggagata
    25921 atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac tgttgctgtt
    25981 cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg gttatagatt
    26041 tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac ttctaatgta
    26101 gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt agtttttcca
    26161 catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa tttgtatctt
    26221 tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt tcacttatgt
    26281 atattaacgg aattagtaac acaaatattt atataaaaaa atattttact tgtcgttttg
    26341 gcttttcatt tttgttataa tagtcattaa ttataaatag aaagcttttt atataaaaag
    26401 agaaaggaaa gtggttcttt tgttaacaaa caaccaatcc tccaatcttg tcaaaaaaac
    26461 actaacctat tccatagata cccatcttca tttatctgct gctccctatt ttcgactact
    26521 aataaatgaa agcgctaatt tatatgctga atttgcacat aaatctatag aactagataa
    26581 tccttccctt ttattcattg ataaacagca agaagtccaa atcttttcta ctcaaaagga
    26641 aacacaaact tttatcatta tccagtttta ttttgatgag caattattag cacatattac
    26701 gggacaatct ttttctatag atcaagaaat aacatctatc attacttttc tgaaaaatga
    26761 acagttcaaa attgattatc taaaacatag taagccagct atttcctatg atcaggaaat
    26821 caattctttt ttcatactat gccaacagat gagcattcat ttgctttcct tattagttac
    26881 ttattgtctg caaaaaattc aattgtcttc cttaccaagg cattatcaac aggaacttgc
    26941 tttattgaca ataccttcaa atgagaaagt cattcctaaa aaaaagcata ttctttattc
    27001 agatgaatta gttgatccaa tcatacgata tttagaagaa catctttcag aaaatttttt
    27061 gcttgaacaa ttagcccaaa ttttttttat cagtccagcc agcttaaaaa aacattttag
    27121 aaaagtgaat ggaaaaagtg ttattagcta ttttaaagaa ctaaaaatgg aaaaagcaaa
    27181 acgtctcgtg ctagaaaatg aattaaccta tacagaaatt gcagatatat taggatttca
    27241 ttcgatccat catttttctt cagcttttaa aaaatataca gggttatccc cttcaaagta
    27301 ttatctatcc ttaaaataga aaaaaatttt taggttttat ccacaaatta tttctctata
    27361 ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc
    27421 ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc
    27481 ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa
    27541 atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat
    27601 ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa
    27661 acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact
    27721 aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag
    27781 ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca
    27841 gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa
    27901 gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct
    27961 gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct
    28021 ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta
    28081 gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta
    28141 tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag
    28201 aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat
    28261 gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact
    28321 agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga
    28381 catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca
    28441 ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta
    28501 aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat
    28561 ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt
    28621 attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct
    28681 gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct
    28741 ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa
    28801 atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa
    28861 gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca
    28921 aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa
    28981 gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt
    29041 ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg
    29101 tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg
    29161 tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt
    29221 aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt
    29281 cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt
    29341 aactacgcct aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc
    29401 atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc
    29461 aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta
    29521 ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa
    29581 tgtgtgtatt aaataattta atttttttgt tcctatttat ttaa
//
LOCUS       DACECS010000026        28200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.026, whole genome shotgun sequence.
ACCESSION   DACECS010000026 DACECS010000000
VERSION     DACECS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.026"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(142..837)
                     /locus_tag="IWO31_001319"
     CDS             complement(142..837)
                     /locus_tag="IWO31_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0301745.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(927..2027)
                     /gene="ychF"
                     /locus_tag="IWO31_001320"
     CDS             complement(927..2027)
                     /gene="ychF"
                     /locus_tag="IWO31_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0301746.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2036..2227)
                     /locus_tag="IWO31_001321"
     CDS             complement(2036..2227)
                     /locus_tag="IWO31_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0301747.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2248..3138)
                     /locus_tag="IWO31_001322"
     CDS             complement(2248..3138)
                     /locus_tag="IWO31_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0301748.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3125..3892)
                     /locus_tag="IWO31_001323"
     CDS             complement(3125..3892)
                     /locus_tag="IWO31_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0301749.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3904..4617)
                     /gene="rsmG"
                     /locus_tag="IWO31_001324"
     CDS             complement(3904..4617)
                     /gene="rsmG"
                     /locus_tag="IWO31_001324"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0301750.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4849..6750)
                     /gene="mnmG"
                     /locus_tag="IWO31_001325"
     CDS             complement(4849..6750)
                     /gene="mnmG"
                     /locus_tag="IWO31_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0301751.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6767..8164)
                     /gene="mnmE"
                     /locus_tag="IWO31_001326"
     CDS             complement(6767..8164)
                     /gene="mnmE"
                     /locus_tag="IWO31_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0301752.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8508..8846
                     /locus_tag="IWO31_001327"
     CDS             8508..8846
                     /locus_tag="IWO31_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301753.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9014..9772)
                     /locus_tag="IWO31_001328"
     CDS             complement(9014..9772)
                     /locus_tag="IWO31_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0301754.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9790..10608)
                     /locus_tag="IWO31_001329"
     CDS             complement(9790..10608)
                     /locus_tag="IWO31_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0301755.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10605..10961)
                     /gene="rnpA"
                     /locus_tag="IWO31_001330"
     CDS             complement(10605..10961)
                     /gene="rnpA"
                     /locus_tag="IWO31_001330"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0301756.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11079..11213)
                     /gene="rpmH"
                     /locus_tag="IWO31_001331"
     CDS             complement(11079..11213)
                     /gene="rpmH"
                     /locus_tag="IWO31_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0301757.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11736..13070
                     /gene="dnaA"
                     /locus_tag="IWO31_001332"
     CDS             11736..13070
                     /gene="dnaA"
                     /locus_tag="IWO31_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0301758.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13268..14398
                     /gene="dnaN"
                     /locus_tag="IWO31_001333"
     CDS             13268..14398
                     /gene="dnaN"
                     /locus_tag="IWO31_001333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0301759.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14627..14869
                     /gene="yaaA"
                     /locus_tag="IWO31_001334"
     CDS             14627..14869
                     /gene="yaaA"
                     /locus_tag="IWO31_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0301760.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14856..15980
                     /gene="recF"
                     /locus_tag="IWO31_001335"
     CDS             14856..15980
                     /gene="recF"
                     /locus_tag="IWO31_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0301761.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15977..17923
                     /gene="gyrB"
                     /locus_tag="IWO31_001336"
     CDS             15977..17923
                     /gene="gyrB"
                     /locus_tag="IWO31_001336"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0301762.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17943..20414
                     /gene="gyrA"
                     /locus_tag="IWO31_001337"
     CDS             17943..20414
                     /gene="gyrA"
                     /locus_tag="IWO31_001337"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0301763.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20650..20949
                     /locus_tag="IWO31_001338"
     CDS             20650..20949
                     /locus_tag="IWO31_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0301764.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20999..21511
                     /gene="ssb"
                     /locus_tag="IWO31_001339"
     CDS             20999..21511
                     /gene="ssb"
                     /locus_tag="IWO31_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0301765.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21537..21773
                     /gene="rpsR"
                     /locus_tag="IWO31_001340"
     CDS             21537..21773
                     /gene="rpsR"
                     /locus_tag="IWO31_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0301766.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21916..23889
                     /locus_tag="IWO31_001341"
     CDS             21916..23889
                     /locus_tag="IWO31_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0301767.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23895..24347
                     /gene="rplI"
                     /locus_tag="IWO31_001342"
     CDS             23895..24347
                     /gene="rplI"
                     /locus_tag="IWO31_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0301768.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24621..25988
                     /gene="dnaB"
                     /locus_tag="IWO31_001343"
     CDS             24621..25988
                     /gene="dnaB"
                     /locus_tag="IWO31_001343"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0301769.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26768..28060
                     /locus_tag="IWO31_001344"
     CDS             26768..28060
                     /locus_tag="IWO31_001344"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0301770.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8591 a         5528 c         5602 g         8479 t
ORIGIN      
        1 ttttgaacac cgtttatcgt aaaaagggac tgtgacaaga cttttgtcac agtcccttac
       61 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
      121 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      181 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      241 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      301 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      361 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      421 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      481 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      541 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      601 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      661 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      721 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      781 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      841 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      901 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      961 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
     1021 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1081 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1141 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1201 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1261 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1321 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1381 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1441 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1501 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1561 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1621 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1681 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1741 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1801 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1861 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1921 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1981 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     2041 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2101 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2161 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2221 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2281 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2341 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2401 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2461 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2521 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2581 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2641 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2701 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2761 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2821 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2881 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2941 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     3001 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3061 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3121 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3181 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3241 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3301 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3361 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3421 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3481 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3541 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3601 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3661 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3721 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3781 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3841 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3901 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3961 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     4021 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4081 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4141 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4201 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4261 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4321 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4381 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4441 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4501 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4561 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4621 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4681 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4741 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4801 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4861 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4921 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4981 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     5041 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5101 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5161 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5221 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5281 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5341 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5401 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5461 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5521 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5581 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5641 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5701 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5761 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5821 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5881 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5941 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     6001 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6061 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6121 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6181 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6241 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6301 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6361 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6421 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6481 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6541 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6601 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6661 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6721 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6781 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6841 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6901 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6961 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     7021 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7081 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7141 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7201 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7261 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7321 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7381 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7441 ttgatttgcc gacatttggc cggccgatga ttgccgtact taacccttcc cgcagaacct
     7501 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7561 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7621 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7681 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7741 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7801 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7861 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7921 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7981 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     8041 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8101 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8161 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8221 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8281 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8341 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8401 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8461 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8521 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8581 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8641 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8701 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8761 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8821 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8881 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8941 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     9001 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9061 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9121 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9181 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9241 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9301 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9361 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9421 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9481 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9541 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9601 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9661 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9721 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9781 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9841 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9901 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9961 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
    10021 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10081 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10141 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10201 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10261 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10321 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10381 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10441 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10501 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10561 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10621 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10681 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10741 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10801 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10861 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10921 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10981 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    11041 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11101 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11161 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11221 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11281 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11341 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11401 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11461 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11521 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11581 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11641 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11701 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11761 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11821 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11881 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11941 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    12001 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12061 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12121 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12181 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12241 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12301 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12361 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12421 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12481 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12541 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12601 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12661 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12721 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12781 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12841 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12901 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12961 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    13021 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13081 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13141 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13201 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13261 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13321 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13381 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13441 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13501 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13561 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13621 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13681 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13741 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13801 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13861 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13921 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13981 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    14041 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14101 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14161 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14221 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14281 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14341 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14401 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14461 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14521 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14581 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14641 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14701 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14761 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14821 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14881 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14941 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    15001 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15061 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15121 aaaaaggccg aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg
    15181 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15241 agaccaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15301 atcttgtcca ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg
    15361 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15421 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15481 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15541 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15601 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15661 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15721 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg
    15781 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc
    15841 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15901 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15961 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    16021 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16081 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16141 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16201 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16261 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16321 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16381 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16441 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16501 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16561 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16621 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16681 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16741 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16801 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16861 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16921 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16981 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    17041 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17101 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17161 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17221 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17281 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17341 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17401 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17461 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17521 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17581 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17641 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17701 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17761 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17821 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17881 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17941 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    18001 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18061 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18121 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18181 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18241 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18301 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18361 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18421 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18481 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18541 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18601 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18661 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18721 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18781 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18841 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18901 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18961 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    19021 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19081 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19141 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19201 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19261 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19321 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19381 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19441 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19501 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19561 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19621 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19681 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19741 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19801 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19861 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19921 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19981 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    20041 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20101 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20161 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20221 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20281 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20341 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20401 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20461 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20521 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20581 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20641 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20701 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20761 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20821 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20881 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20941 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    21001 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21061 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21121 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21181 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21241 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21301 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21361 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21421 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21481 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21541 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21601 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21661 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21721 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21781 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21841 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21901 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21961 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    22021 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22081 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22141 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22201 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22261 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22321 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22381 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22441 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22501 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22561 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22621 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22681 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22741 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22801 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22861 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22921 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22981 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    23041 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23101 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23161 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23221 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23281 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23341 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23401 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23461 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23521 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23581 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23641 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23701 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23761 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23821 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23881 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23941 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    24001 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24061 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24121 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24181 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24241 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24301 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24361 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24421 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24481 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24541 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24601 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24661 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24721 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24781 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24841 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24901 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24961 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    25021 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25081 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25141 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25201 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25261 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25321 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25381 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25441 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25501 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25561 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25621 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25681 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25741 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25801 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25861 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25921 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25981 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    26041 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26101 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26161 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26221 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26281 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26341 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26401 atctgcggta atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt
    26461 cttattactt gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta
    26521 gcttcttcga aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc
    26581 cttcttattt cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa
    26641 aaagataaag ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt
    26701 atttgaaata tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg
    26761 tgttcacatg tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa
    26821 aatcacagac tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa
    26881 tgcagggcac acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg
    26941 gattttttat aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc
    27001 attggttaaa gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat
    27061 ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga
    27121 tgctaagggc gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga
    27181 taaagcagct cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga
    27241 acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga
    27301 accacttgat tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca
    27361 atacgtcaca gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt
    27421 gtttgaaggc gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac
    27481 ttcttctaac ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat
    27541 caataaagtt gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc
    27601 aactgaatta tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac
    27661 gacaactggt cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa
    27721 acgagtttca ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga
    27781 aacagtaaaa atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc
    27841 aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga
    27901 agacattaca ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg
    27961 tcgtatctca gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac
    28021 acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa
    28081 tcaggacgtt cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa
    28141 cactgtttat tcagaataag ggactgtgac aaaaaccgtg tcacagtccc tttttccgaa
//
LOCUS       DACECS010000027        27534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.027, whole genome shotgun sequence.
ACCESSION   DACECS010000027 DACECS010000000
VERSION     DACECS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.027"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IWO31_001345"
     CDS             complement(26..466)
                     /locus_tag="IWO31_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0301771.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IWO31_001346"
     CDS             626..1132
                     /locus_tag="IWO31_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0301772.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IWO31_001347"
     CDS             1300..3213
                     /locus_tag="IWO31_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0301773.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IWO31_001348"
     CDS             3218..4219
                     /locus_tag="IWO31_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0301774.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IWO31_001349"
     CDS             complement(4421..4627)
                     /locus_tag="IWO31_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0301775.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5273..6121)
                     /locus_tag="IWO31_001350"
     CDS             complement(5273..6121)
                     /locus_tag="IWO31_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0301776.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6285..7187
                     /gene="gnd"
                     /locus_tag="IWO31_001351"
     CDS             6285..7187
                     /gene="gnd"
                     /locus_tag="IWO31_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0301777.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            7189..8190
                     /locus_tag="IWO31_001352"
     CDS             7189..8190
                     /locus_tag="IWO31_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0301778.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8187..8471
                     /locus_tag="IWO31_001353"
     CDS             8187..8471
                     /locus_tag="IWO31_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0301779.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            8504..9301
                     /locus_tag="IWO31_001354"
     CDS             8504..9301
                     /locus_tag="IWO31_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0301780.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9315..10148
                     /locus_tag="IWO31_001355"
     CDS             9315..10148
                     /locus_tag="IWO31_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0301781.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10166..11023
                     /locus_tag="IWO31_001356"
     CDS             10166..11023
                     /locus_tag="IWO31_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0301782.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11045..11554
                     /locus_tag="IWO31_001357"
     CDS             11045..11554
                     /locus_tag="IWO31_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0301783.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            11591..11992
                     /locus_tag="IWO31_001358"
     CDS             11591..11992
                     /locus_tag="IWO31_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301784.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12104..12457
                     /locus_tag="IWO31_001359"
     CDS             12104..12457
                     /locus_tag="IWO31_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301785.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            12491..12934
                     /locus_tag="IWO31_001360"
     CDS             12491..12934
                     /locus_tag="IWO31_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0301786.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            12951..14036
                     /gene="uxuA"
                     /locus_tag="IWO31_001361"
     CDS             12951..14036
                     /gene="uxuA"
                     /locus_tag="IWO31_001361"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0301787.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14029..14742
                     /locus_tag="IWO31_001362"
     CDS             14029..14742
                     /locus_tag="IWO31_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0301788.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            15494..15694
                     /locus_tag="IWO31_001363"
     CDS             15494..15694
                     /locus_tag="IWO31_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0301789.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            16262..16825
                     /locus_tag="IWO31_001364"
     CDS             16262..16825
                     /locus_tag="IWO31_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301790.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(16886..17251)
                     /locus_tag="IWO31_001365"
     CDS             complement(16886..17251)
                     /locus_tag="IWO31_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301791.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(17327..17572)
                     /locus_tag="IWO31_001366"
     CDS             complement(17327..17572)
                     /locus_tag="IWO31_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0301792.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(17642..17896)
                     /locus_tag="IWO31_001367"
     CDS             complement(17642..17896)
                     /locus_tag="IWO31_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301793.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(18060..18383)
                     /locus_tag="IWO31_001368"
     CDS             complement(18060..18383)
                     /locus_tag="IWO31_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301794.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            18570..18643
                     /locus_tag="IWO31_001369"
     tRNA            18570..18643
                     /locus_tag="IWO31_001369"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18604..18606,aa:Ile,seq:cat)
     gene            complement(19028..19525)
                     /locus_tag="IWO31_001370"
     CDS             complement(19028..19525)
                     /locus_tag="IWO31_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0301795.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(19565..20374)
                     /locus_tag="IWO31_001371"
     CDS             complement(19565..20374)
                     /locus_tag="IWO31_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0301796.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            20708..21565
                     /locus_tag="IWO31_001372"
     CDS             20708..21565
                     /locus_tag="IWO31_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0301797.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            21558..22460
                     /locus_tag="IWO31_001373"
     CDS             21558..22460
                     /locus_tag="IWO31_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0301798.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            22493..22843
                     /locus_tag="IWO31_001374"
     CDS             22493..22843
                     /locus_tag="IWO31_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0301799.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            22877..23814
                     /locus_tag="IWO31_001375"
                     /pseudo
     CDS             22877..23814
                     /locus_tag="IWO31_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            23892..24785
                     /locus_tag="IWO31_001376"
     CDS             23892..24785
                     /locus_tag="IWO31_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0301800.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            24823..25386
                     /gene="amaP"
                     /locus_tag="IWO31_001377"
     CDS             24823..25386
                     /gene="amaP"
                     /locus_tag="IWO31_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0301801.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            25400..25588
                     /locus_tag="IWO31_001378"
     CDS             25400..25588
                     /locus_tag="IWO31_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0301802.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            25601..26503
                     /locus_tag="IWO31_001379"
     CDS             25601..26503
                     /locus_tag="IWO31_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0301803.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            26535..26777
                     /locus_tag="IWO31_001380"
     CDS             26535..26777
                     /locus_tag="IWO31_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0301804.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(27033..27404)
                     /locus_tag="IWO31_001381"
     CDS             complement(27033..27404)
                     /locus_tag="IWO31_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301805.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         9628 a         4447 c         5559 g         7900 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa
     4981 ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa
     5041 taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac
     5101 aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac
     5161 ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat
     5221 ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt
     5281 tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt
     5341 aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta
     5401 aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt
     5461 tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta
     5521 cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac
     5581 agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag
     5641 aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac
     5701 cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta
     5761 gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca
     5821 ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag
     5881 cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac
     5941 caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat
     6001 tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct
     6061 gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca
     6121 tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag
     6181 atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa
     6241 aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa
     6301 ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag
     6361 tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg
     6421 cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa
     6481 tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac
     6541 caggagacat tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag
     6601 aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag
     6661 gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg
     6721 aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa
     6781 gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg
     6841 gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac
     6901 gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt
     6961 taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa
     7021 aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt
     7081 tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac
     7141 taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat
     7201 catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga
     7261 ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa
     7321 ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga
     7381 cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac
     7441 aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct
     7501 ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga
     7561 cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc
     7621 gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc
     7681 ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac
     7741 aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg
     7801 gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt
     7861 tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc
     7921 gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat
     7981 gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa
     8041 caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt
     8101 gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc
     8161 cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga
     8221 aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct
     8281 tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg
     8341 cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt
     8401 ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat
     8461 cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa
     8521 tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac
     8581 acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta
     8641 cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata
     8701 tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga
     8761 acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg
     8821 caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact
     8881 gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg
     8941 attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa
     9001 atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta
     9061 tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga
     9121 attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat
     9181 agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt
     9241 aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg
     9301 aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg
     9361 tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc
     9421 ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg
     9481 ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag
     9541 ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc
     9601 cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt
     9661 ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta gaagatttga
     9721 atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat
     9781 tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca
     9841 aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg
     9901 caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct
     9961 tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg
    10021 ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac
    10081 agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg
    10141 gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc
    10201 tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga
    10261 aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat
    10321 cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact
    10381 gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat
    10441 ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt
    10501 gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga
    10561 ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag
    10621 tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt
    10681 atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac
    10741 tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct
    10801 tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt
    10861 tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat
    10921 gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct
    10981 gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa
    11041 taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat
    11101 ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc
    11161 aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac
    11221 aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata
    11281 caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat
    11341 ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt
    11401 agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga
    11461 acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt
    11521 aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga
    11581 gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat
    11641 ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga
    11701 tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc
    11761 aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga
    11821 tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa
    11881 aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat
    11941 gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat
    12001 cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc
    12061 tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg
    12121 tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa
    12181 gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc
    12241 tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg
    12301 gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa
    12361 aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg
    12421 gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt
    12481 gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa
    12541 agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc
    12601 tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac
    12661 agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct
    12721 cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt
    12781 gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga
    12841 agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc
    12901 ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg
    12961 gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac
    13021 cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga
    13081 tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa
    13141 ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga gattactata
    13201 ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt
    13261 atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg
    13321 gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt
    13381 atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt
    13441 tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg
    13501 aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa
    13561 tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa
    13621 atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc
    13681 tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg
    13741 taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt
    13801 tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag
    13861 cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg
    13921 gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc
    13981 aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc
    14041 aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt
    14101 gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta
    14161 ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat
    14221 gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa
    14281 gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga
    14341 ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa
    14401 atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga
    14461 aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa
    14521 gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg
    14581 tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg
    14641 aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat
    14701 caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct
    14761 agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt
    14821 tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct
    14881 actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt
    14941 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
    15001 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
    15061 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
    15121 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
    15181 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
    15241 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
    15301 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
    15361 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
    15421 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
    15481 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
    15541 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
    15601 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
    15661 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
    15721 cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata
    15781 gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata
    15841 aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa
    15901 tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat
    15961 taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag
    16021 taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg
    16081 aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag
    16141 aatgagatct aataaaacct gatctttcat ctgttttttt aataacgtaa taattttttt
    16201 attttttgtg ctttcgccgt tattgtagag tgatttagca ttcttatttt taaaaataac
    16261 tgtgttatat attaaaaaag gcataatagt tataagcttt tttaaagaat atttagaaat
    16321 tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct attttcaggg
    16381 ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag caatggcagg
    16441 ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag ccacgacaga
    16501 ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac gatgcatggg
    16561 aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag
    16621 caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat cgtatacaga
    16681 cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt ctagggaagt
    16741 tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg aagataaccc
    16801 taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat gaagtaagag
    16861 gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc tccaaatttg
    16921 ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg ttgcgctcta
    16981 cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga tttttccact
    17041 ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga tgatattgga
    17101 ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc tttccccagc
    17161 catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga cgaggaagct
    17221 ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat atagattgtt
    17281 aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc
    17341 ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag tcagcgatat
    17401 agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga tatttgaata
    17461 aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca gttcttaaga
    17521 aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc atttaatcac
    17581 ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca atcaatagta
    17641 ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa gacaaaacca
    17701 gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt
    17761 tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt tctttgttcc
    17821 aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc
    17881 tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag ataacatgtt
    17941 attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat
    18001 gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc
    18061 tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg
    18121 atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca
    18181 aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga
    18241 agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc tgaggtttga
    18301 taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat cgcaataaat
    18361 ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt ttctaaaata
    18421 agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa taagaataac
    18481 aatattattt tacttatatt ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa
    18541 aagtaacaaa aataaatttt atttatgatg tacctttagc tcagttggtt agagcagacg
    18601 gctcataacc gtccggtcgt aggttcgagt cctacaaggt acataagagg ttgtgaaaaa
    18661 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt
    18721 ggctgttctt attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa
    18781 atcagcgttt tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc
    18841 cgtttgttaa gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg
    18901 agcctgccgt ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt
    18961 aggactaaaa cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata
    19021 gatattttta tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt
    19081 ctccgtgttt tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag
    19141 cactctgcag gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt
    19201 cgatctcttt ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt
    19261 caggtggaat cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggagaaagaa
    19321 gagggatcaa ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat
    19381 cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac
    19441 gttctgccaa ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat
    19501 tttgcatatc aataataact aacattttct ttcctcctac attcatatcg tcattttatt
    19561 ctttttaata gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta
    19621 cttggtttgc aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc
    19681 tgtaattcaa catagcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt
    19741 cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg
    19801 tgatttctaa gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc
    19861 tttgtagctt ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa
    19921 gagaaggatc ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat
    19981 gttcataata agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt
    20041 gggtttctcg gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc
    20101 tcagtgtatc gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca
    20161 tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa
    20221 aaatatcttt agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt
    20281 catcaaattt ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca
    20341 aggtaccgtc taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat
    20401 atctattttc ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct
    20461 ttgtcattat gaattttttc aaaaaacatt cttaagaaaa aggtggctat atatagtagt
    20521 ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg
    20581 cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta
    20641 aaaccaaatg aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt
    20701 gattttgatg gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta
    20761 tgctccttac ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg
    20821 cataaaattt tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga
    20881 aacggtaaat aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat
    20941 ctacgaagac ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt
    21001 accaaactta ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt
    21061 agtactagac gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc
    21121 tattatcgat ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt
    21181 gtacttcaat tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac
    21241 agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga
    21301 tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca
    21361 gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa
    21421 agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac
    21481 gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac
    21541 gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa
    21601 aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta
    21661 aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac
    21721 gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag
    21781 aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg
    21841 cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca
    21901 tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag
    21961 cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat
    22021 caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa
    22081 atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg
    22141 accaagatgc attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga
    22201 atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg
    22261 aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt
    22321 ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg
    22381 ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa
    22441 cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag gaatggaaat
    22501 tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat
    22561 cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa
    22621 aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat
    22681 cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg
    22741 attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt
    22801 actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc
    22861 aaaaaggagg tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac
    22921 agatgaaaaa aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca
    22981 agcaatcgaa gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga
    23041 agtttctcgg atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt
    23101 gattaatcta gatcagtcag ataatcgtat cgaaaaaaat taacgccaat atcttttgta
    23161 ctttccgata atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg
    23221 ctgacaaagc atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta
    23281 gatatttaca cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg
    23341 gataaagcag ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa
    23401 agagacatcg tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta
    23461 tggaattctg aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat
    23521 ttgagacagc gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt
    23581 ggtgatctat tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat
    23641 tcagctgcat taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca
    23701 ggaggattac cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg
    23761 atagcagcag tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc
    23821 tatttaaaac aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga
    23881 ggaatacagc aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga
    23941 aatttcctaa acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg
    24001 attgtggaga agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca
    24061 ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag
    24121 atatcgctct tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg
    24181 tgaaagttgc aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac
    24241 ctaaagaaat catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa
    24301 acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag
    24361 acacatttat gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata
    24421 tcccagcagg aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac
    24481 atttattaga ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa
    24541 aacagcttgc acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac
    24601 cattacaact agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt
    24661 tactagaacg tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga
    24721 ataaagcaag ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact
    24781 tataagaagt tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa
    24841 acgatcggag tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta
    24901 agtgcaacct atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat
    24961 tcatacgtgc aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc
    25021 acgttacttg ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt
    25081 gatggggagt tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat
    25141 gaagatgaat tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc
    25201 gtcaatgtaa aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg
    25261 atgaaagaaa tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta
    25321 tcggtgaaat acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta
    25381 gaataggggg ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt
    25441 tggggtactt ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt
    25501 attgatcttg atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa
    25561 aatcttagat cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa
    25621 taataacaat aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc
    25681 aaaccaatca aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc
    25741 agatccagta gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa
    25801 gcatgcacct catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat
    25861 cgatccttct aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga
    25921 aaaacttagt tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa
    25981 ccaaggaaag catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga
    26041 taaagttgtg caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc
    26101 aaacggtggt ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc
    26161 tgggatcgaa acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga
    26221 atatggcaaa gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt
    26281 aaaaaatatg acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac
    26341 aaaagaagaa tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa
    26401 aaaaaccggt gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg
    26461 cgcacaaaaa gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt
    26521 aggaggaacg aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa
    26581 ttgcaggagc aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag
    26641 gtttagttgg atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag
    26701 gaatggcaat cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct
    26761 ttgctcgaaa actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca
    26821 agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt
    26881 atcgaagatg caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt
    26941 tgtcacagcc tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt
    27001 aaatgtgtct ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt
    27061 cttgagatag gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc
    27121 cacaaatgga tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga
    27181 ttgaaaaaga caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa
    27241 tagctcgtaa agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca
    27301 atcgtatcat gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata
    27361 tagatcacga gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt
    27421 gtgtgagttc gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt
    27481 aacaaatgtt atctaatgta tttttaaata aataatcatt attatcaaaa taat
//
LOCUS       DACECS010000028        27217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.028, whole genome shotgun sequence.
ACCESSION   DACECS010000028 DACECS010000000
VERSION     DACECS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.028"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            129..992
                     /locus_tag="IWO31_001382"
     CDS             129..992
                     /locus_tag="IWO31_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0301806.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1121..2743)
                     /locus_tag="IWO31_001383"
     CDS             complement(1121..2743)
                     /locus_tag="IWO31_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301807.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2992..3513
                     /locus_tag="IWO31_001384"
     CDS             2992..3513
                     /locus_tag="IWO31_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301808.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3699..5429)
                     /gene="spxB"
                     /locus_tag="IWO31_001385"
     CDS             complement(3699..5429)
                     /gene="spxB"
                     /locus_tag="IWO31_001385"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0301809.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5594..6412
                     /locus_tag="IWO31_001386"
     CDS             5594..6412
                     /locus_tag="IWO31_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0301810.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6515..6712)
                     /locus_tag="IWO31_001387"
     CDS             complement(6515..6712)
                     /locus_tag="IWO31_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301811.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6881..7459
                     /locus_tag="IWO31_001388"
     CDS             6881..7459
                     /locus_tag="IWO31_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0301812.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8504..9007)
                     /locus_tag="IWO31_001389"
     CDS             complement(8504..9007)
                     /locus_tag="IWO31_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0301813.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9366..10391
                     /locus_tag="IWO31_001390"
     CDS             9366..10391
                     /locus_tag="IWO31_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0301814.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10714..11592
                     /locus_tag="IWO31_001391"
     CDS             10714..11592
                     /locus_tag="IWO31_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301815.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12068..12184
                     /locus_tag="IWO31_001392"
     CDS             12068..12184
                     /locus_tag="IWO31_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0301816.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13112..13450
                     /locus_tag="IWO31_001393"
     CDS             13112..13450
                     /locus_tag="IWO31_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301817.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13544..13774
                     /locus_tag="IWO31_001394"
     CDS             13544..13774
                     /locus_tag="IWO31_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301818.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14174..14545
                     /locus_tag="IWO31_001395"
     CDS             14174..14545
                     /locus_tag="IWO31_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0301819.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14819..15511)
                     /locus_tag="IWO31_001396"
     CDS             complement(14819..15511)
                     /locus_tag="IWO31_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0301820.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15514..16299)
                     /locus_tag="IWO31_001397"
     CDS             complement(15514..16299)
                     /locus_tag="IWO31_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0301821.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16536..17936
                     /gene="lacG"
                     /locus_tag="IWO31_001398"
     CDS             16536..17936
                     /gene="lacG"
                     /locus_tag="IWO31_001398"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0301822.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17961..18278
                     /locus_tag="IWO31_001399"
     CDS             17961..18278
                     /locus_tag="IWO31_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0301823.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18291..19964
                     /locus_tag="IWO31_001400"
     CDS             18291..19964
                     /locus_tag="IWO31_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0301824.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19978..21609
                     /locus_tag="IWO31_001401"
     CDS             19978..21609
                     /locus_tag="IWO31_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0301825.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22481..23671
                     /locus_tag="IWO31_001402"
     CDS             22481..23671
                     /locus_tag="IWO31_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0301826.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23805..25994
                     /locus_tag="IWO31_001403"
     CDS             23805..25994
                     /locus_tag="IWO31_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0301827.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            25991..>27217
                     /locus_tag="IWO31_001404"
     CDS             25991..>27217
                     /locus_tag="IWO31_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0301828.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         8733 a         4485 c         5383 g         8616 t
ORIGIN      
        1 ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc ggttaaagtg
       61 tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac gagaaggggt
      121 tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac catgtattag
      181 caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa gagttagtag
      241 atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa aatggtgcga
      301 tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt tcttatgcgg
      361 aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc tcttacagta
      421 gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag atctatgttt
      481 ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct gtaagattga
      541 aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat tatttgaaag
      601 tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt aggatttcca
      661 aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg gtggataaag
      721 aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc cagacaatcg
      781 tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat gtggcttacg
      841 ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag ataaagatga
      901 aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc tatggctgta
      961 tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg taaaaaagcc
     1021 cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat cctatctctt
     1081 agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat aactcgttca
     1141 attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca gctcgatcga
     1201 tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata aattgatggt
     1261 catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc aatgctgtga
     1321 ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta gctttcgaaa
     1381 gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca ttcactggtt
     1441 tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag aaggtgttat
     1501 catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga tcagtatcga
     1561 attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc caacgtactg
     1621 ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt gttgttatat
     1681 cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat atatcatcta
     1741 aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag tcgtttccaa
     1801 tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt tggatatcgt
     1861 ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat tttgatgcat
     1921 tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct tttttggcat
     1981 ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa tcataatttc
     2041 ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat actttgttca
     2101 aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc aaaaattctt
     2161 ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc aaaagaacat
     2221 caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca ccagttaatt
     2281 cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt aaaacagctg
     2341 cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat tcagctgctc
     2401 gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt tctttcagca
     2461 cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag tcttcatagt
     2521 cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta acggaacctg
     2581 tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct gcaccatttg
     2641 caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca tcaaatagtt
     2701 tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt cctcactttt
     2761 atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa aagattcaag
     2821 gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa aataaaagcg
     2881 gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt tgacgttatt
     2941 tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa
     3001 aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact tgtctcattg
     3061 ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt tgcgattttt
     3121 gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga gctaacagga
     3181 tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg cgtctttttc
     3241 ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt atggtttatt
     3301 gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag tgaaggatat
     3361 tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt cttattgttt
     3421 aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt tatgttattt
     3481 ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata aaaaagccgg
     3541 cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat ggacaaatat
     3601 gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga agctatatac
     3661 acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt ttcatataat
     3721 tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg gatttcttct
     3781 tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg cttgttattg
     3841 atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa ggcaaatcga
     3901 agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc aactttaccg
     3961 aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct
     4021 tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag ctgatatttg
     4081 acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact taaggtaaat
     4141 acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc attacccata
     4201 gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa taaacgaata
     4261 gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc agcaatacga
     4321 ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc ttcaaaagat
     4381 ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca gctatcagca
     4441 gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc accgaggatc
     4501 gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat ttggataaat
     4561 tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac aaataagatc
     4621 agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc agcaaaacca
     4681 agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc gacaacagga
     4741 actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca
     4801 acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag caaatctttt
     4861 tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca
     4921 atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt atgctcatag
     4981 gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat gactgtacga
     5041 ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa agagttatag
     5101 ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg gtccatctgt
     5161 gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg
     5221 ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac ttcttcatga
     5281 cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat aattgaattg
     5341 aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag aacttttacc
     5401 attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa tgatgataca
     5461 taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta attcgtcttt
     5521 tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga tagattcaag
     5581 agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc aagcacttgc
     5641 tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt ttttctttaa
     5701 agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg tcatgattgc
     5761 tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa atggagatat
     5821 tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat atatggcaga
     5881 taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat tgcctaccca
     5941 tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc ctgaaggttt
     6001 agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag ccgctatatt
     6061 agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag gagcagctgt
     6121 atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt acggacaagc
     6181 ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa cgaaagtgga
     6241 gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg tcgtggtgga
     6301 agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg cagtttttgg
     6361 cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat aaataaaaaa
     6421 aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat ttttttagta
     6481 agttatatca taatgaagtg aatggtatca tacattaata atagtttttt ctatgtgtat
     6541 ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga actttgaccg
     6601 caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag ctagctgcaa
     6661 ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc atacaaaacc
     6721 ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta aaaacacctc
     6781 caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc tttttctctg
     6841 gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa atcgtcttga
     6901 agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag aacttcatga
     6961 acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt ttatctatat
     7021 tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat ttcaagttgt
     7081 acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg cgctttcgct
     7141 ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc cagaaatgac
     7201 ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa aagaacttgt
     7261 caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt acaagaaatc
     7321 gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag tagcgccagt
     7381 agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa gtcgtaccat
     7441 cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa
     7501 ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg cagatggatc
     7561 aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt tatttggatc
     7621 aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta ttcacagcaa
     7681 taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa agcatgttta
     7741 cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa
     7801 gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa gagataaaaa
     7861 taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat acaacagaaa
     7921 tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg gaataaaaaa
     7981 ataacatatc tcattttacg cttttgttat tttatactta aaaaataacg acacagaaaa
     8041 taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc tttgaaatgc
     8101 cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg gagcctaaat
     8161 aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt tttatagaaa
     8221 aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat tgagatattg
     8281 actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt tttgttaaat
     8341 aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag
     8401 attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc gtcatagata
     8461 cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga agacggacag
     8521 ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat cccaaaataa
     8581 tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga taggcccata
     8641 aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct acgccaatac
     8701 cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc cctaattttt
     8761 tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga aaaaagacaa
     8821 tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag
     8881 ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta aatgatagct
     8941 tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat tttgatacat
     9001 accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg aaaaatcata
     9061 aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa gctttttttt
     9121 gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa aagttgctat
     9181 cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct ccaagttgtt
     9241 tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta ggtaatgctg
     9301 acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta ggaaggaggg
     9361 atagattgtt caagacttat tttcgtttca ataaaataca agataatcct tttcgtatca
     9421 tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat catgattttt
     9481 tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa aacataccga
     9541 tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt gctttcgttt
     9601 tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct ttggttgctt
     9661 tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc ttgttttttg
     9721 cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta ccgatcgtct
     9781 cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt acaaaattac
     9841 tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga tttcacgaag
     9901 agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt tccgtttatg
     9961 tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt gcatcgattt
    10021 tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg attggatcga
    10081 tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag ttgcagacca
    10141 tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga aattatgcag
    10201 ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt cgtggagcat
    10261 acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt ttcatcggtt
    10321 taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt gttcgtcaaa
    10381 aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca
    10441 acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat tcacacaaaa
    10501 atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat tttgtttatg
    10561 atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt cttacagata
    10621 ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt atgtttatac
    10681 catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat gaaaagggga
    10741 aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt aactcttccc
    10801 aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag
    10861 caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac
    10921 aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc agcttctcaa
    10981 agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt ggcaacaatg
    11041 aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta tcgctttgac
    11101 cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg tgaattggag
    11161 tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg aaagcagtgc
    11221 ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag agatagttac
    11281 gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca agtgatgtac
    11341 acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc
    11401 tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga tagtggagtc
    11461 ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag tacgagttta
    11521 cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg cttaggtatt
    11581 cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga
    11641 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    11701 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    11761 tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt
    11821 tcaataggta aactagctat aacaatagat attaaagtta acggacaaga gggccttttt
    11881 taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca atcagctagt
    11941 ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat ttaaaacaca
    12001 aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa gaaagtgggt
    12061 cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa gcccaaatat
    12121 caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata gtgggaaaag
    12181 gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa
    12241 taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat ttttaacata
    12301 tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc cagttatata
    12361 gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta gttatttttc
    12421 aattcccttt caacctctta ttttgaccag tagtattgat cactttacct tttaacacct
    12481 ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata atcaaaggtt
    12541 ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg caaataacca
    12601 ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag ctcactccat
    12661 ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa aaagttaact
    12721 ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact attgctagaa
    12781 taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt gtgtattgga
    12841 attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac tattacaagt
    12901 atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat gaatagttgt
    12961 agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca atttgtgatt
    13021 ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg tagtgtttat
    13081 tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa gtgaggaaca
    13141 tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata ttgttccgga
    13201 gactccatta gctagtgagc caattactga tattgatgga aaccaaatag gatggtatat
    13261 acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag taggcttaga
    13321 gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg atgaaagtga
    13381 cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa agtatatatt
    13441 gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa tttactagat
    13501 gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg tagaaaaggg
    13561 aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa
    13621 agaattcaag agtcgctact caattaactc gactccgaca tttacagttt acagagaagg
    13681 atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag ctgaagtaga
    13741 agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg aaaaattttg
    13801 acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta actttaatgc
    13861 agtataaaga gtatccagag tattttattt taacttttgt atatttagta agtagctaca
    13921 cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc ttaattagct
    13981 tattgattct ataatatttt tgatttatat aggaaattaa aaatattata gaatcaacac
    14041 tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa tttctcctgg
    14101 ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact ttgcggagta
    14161 tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca tcaatcaatc
    14221 ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca ttatttcttc
    14281 agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata aagacaaaat
    14341 tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa
    14401 tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag atacggaaac
    14461 gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc agcttggtgg
    14521 agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag tatatcaaaa
    14581 aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca ctatttttaa
    14641 tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat
    14701 acactgctat atttcactaa ggatatagca gtgtatcttt catgtcgttt cactggcagt
    14761 tttttataaa aggaggtgtg catttcagtt gcttttcttt ggtagttagt tgaatcgttt
    14821 aagaattaac ggatgtggca gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg
    14881 ttccatcttt atttagctgt gcctgttcaa atactgtttt gtttttaccg aatggatcaa
    14941 ttgcaataat tgttccgatc cccgcacgtt tggcagcaag taatcctgag taagcatctt
    15001 cgataacaag acaatcagcc ggagctaagc tcagtttgtc tgccgcttta agaaaaatat
    15061 ccggttgcgg tttcccaggg aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc
    15121 agcgggctaa atcgaaaata tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg
    15181 caatcgttaa tggaatctgt tggtcagtca gctggtcaag agtcgcttca agcccttttg
    15241 tcaactgaag ctcgttttca ttttgcaagc aaagcatacg ataatagctt tctttttctt
    15301 ctgacaatac ggcgatctca gaggcggtca aatgctctga aatgaaatgt ttcaaaatct
    15361 ccgtattcgt ccgaccatga atatttttta aaatatcctc gtctgataaa ccatcttttg
    15421 tatgatcatg tatcatatgc agccaagcag cttcatgcag atgagagtcc aaaaacattg
    15481 ttccgttgaa atcaaatata atccctttca tagttacgct cctatagtgg tattttcggt
    15541 tgaatcagca atttgcagat cgacatattg tgcatagtgc tcttgaacct cccgcgagat
    15601 ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta taaaacacat aaaaatcatc
    15661 tcgattgaat tttttattat caacgagtag atatttctta cgggaattat ttagagcgat
    15721 ctgctgtgct tctccttctt cggtactgta agtagagatc gcttcgttgt gaacgccatt
    15781 tgaactgata aatgcttttg taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc
    15841 gacaaatgcg ccggtatttt tccggaaatc accgccaatc agtagaattt ctggtccatc
    15901 tgcttgagca agaatatcaa aaactggata attatttgta atgatccgaa ttttcttgtt
    15961 cagtaaatgc gtcgccaata gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc
    16021 ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt ttttcttgaa tctgcagagt
    16081 ggctttttca gtgtgagaaa gttcttgatc taaagtgtag gaaagacttt gagcccctcc
    16141 atggacacgc agcagcttgc ccgatttttc taattcatct aaatctctgc gaacggtcat
    16201 atcagaaaca tctaattgat tgatgatttc attaacggta aggattcctt ttttattgac
    16261 catttctacg atagtaagca atctttcttt tttcaacatt gtcttcacct ctaatttaag
    16321 gataatctat catataaacg gacaacagtc aatctaattc gaacaaaagt aaacatttag
    16381 ggtggttttt atgtttgtta caatagattt tattgctgtt ttctggtttt tatgattatt
    16441 tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac aatgcaatca gttagaaaga
    16501 gcgaaaactg attatagatt tgaggagaga tgtacatgct taaattacca gaagatttta
    16561 tttttggcgg agccacagca gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag
    16621 gcgctgttgc ttgggatgac tttttagagg aacaagggcg attcagtccc gatccggcaa
    16681 gtgattttta tcatcaatat gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg
    16741 gtctaagatt atccattgct tggagccgca tttttcctga tggagctggg aaaccaaatc
    16801 cagaaggaat cgctttttat catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc
    16861 cgtttgtgac gttgcatcat tttgataccc ctaaacgttt gtttgaccat ggagattttt
    16921 tgaatcgaga aacaatagaa gcatttgttt cctatgccat tttttgtttt catgaattca
    16981 aagaagtcaa ggtgtggagt acctttaatg aaatctatcc agttgcaacc aatcaatatt
    17041 tgttaggtgt tttcccacca ggaatcaaat atgattttac aaagatcgtc gcctgtcttc
    17101 ataatatgat ggttgctcat gcccgtgtag tgaactattt caaagaaaac gaacttccgg
    17161 gtgaaattgg tgtagttcac tcattagaga cgaaatatgc ggcaacagac gcgccggaag
    17221 ataagcatgc tgccttttta gacgatgcat tgtcgatccg tttcttatta gatgctacct
    17281 atttaggcta ttattcaact gaaaccttga ctgctcttga tgagatatgt gaagcaaatc
    17341 aggccagcta tcattttcct gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta
    17401 atgactactt agggatcaat cactaccaat gccattttgt caaagcttac gatggagaaa
    17461 atgcaatcca tcataacggt acgggtgaaa aagggacctc tgtttataaa gtgaagggaa
    17521 tcggagaacg tatttataaa gaaggaattc ctcgaactga ttgggactgg ctgatttatc
    17581 cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa
    17641 tctatatcac tgagaacggg atggggtata aagatcagtt tgaagatggc attattatgg
    17701 atcagccgag aatcgactat ttgagagtct atttggaatc cttaagcaag gcgataacag
    17761 ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat ggatcttttc tcatggacaa
    17821 atgggtataa caaaaggtat ggcttgtttt atgttgattt tgaaacccag aagcgttacc
    17881 caaaagaatc agcttactgg tacaagttgg tcagtgaaac caaaacgatt atttaataag
    17941 tgataatgga ggaagaaaag atgaataaag aggaattaca aatgcttggt tttgagatcg
    18001 ttgcatattc aggcgacgct cgcagtacat tattgaaatt attgaaagaa gtaagacaag
    18061 gaaactttga acatgttgat tcggcgttga aagaagccga cgaaaattta acccttgcac
    18121 ataattcgca aacaaaaatt ttagcggaag aagcatctgg gaaagagatg gagatgggat
    18181 tcatttttat ccatgggcag gatcatttaa tgaccacttt attgctgaga gatctgattc
    18241 aagactttat tgtactgtac agacagaatc aaggatagga gaagaacgct atggatggaa
    18301 tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa aatctcacgt aatagttatt
    18361 taggagccgt tcgtgacgga tttttaacag cgatgccagc tattttattt tcaagcattt
    18421 tcattttgat tgctgcggtt cctgagattt ttggttacca atggcctgaa tctgtctcca
    18481 catggctatg gaaagtctat aattattcga tgggtgttgt agggttactc gtttcagcaa
    18541 ccactgctcg ctgcttggct gattcgatga atcgtcgctt accgaaaaac aaagtaatca
    18601 acaccacctc tgtgatgctg gcctctgtgg taagtttcct gttgttaagc gtctctccga
    18661 ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg actactggct tcctttgtcg
    18721 ctgcattttt aacagttaat ttatataagt tttgtgtgct gaaagatatt accattaaaa
    18781 tgcctaaaga agttcctgga acgatttcgc aaaccttccg agatattttt cctttctctt
    18841 tttcagtatt tgcggccgtt ttaattgatt taggcattcg acaaatcttc caaatctcat
    18901 ttgcagaagc aatcattacg gtgctgcagc cgctatttac agcagctgac gggtacttag
    18961 gaatcgctat tatctgggga gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt
    19021 cgatcgtgga gccggcaatt gcggcaatta tctatgccaa tgtggaaacc aatctgcaat
    19081 tgttcaattc cggtgaacaa gcttctaaag tactgacggt gggtttaggg aattttgtag
    19141 gaactatggg aggaactggg gcaacattgg ttgttccttt tatcttccta ctctttgcta
    19201 agtctaagca gttgaaggca gttgggaaag cttcctttat tccagtaagt ttcgctgtca
    19261 acgaaccatt gctatttgcg gcacccatga ttttgaaccc ttattttttt attccatttt
    19321 tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga
    19381 acagctttat gtacgttttg ccttgggcaa ctccagcacc gatcggtttg gttctaggaa
    19441 cgggcatggg tatcttagct tttgttctag cagcggttct aatcgtcgtt gacttcctga
    19501 tctatctacc attttgcaaa gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag
    19561 cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg
    19621 cagcgagtca agttatcagc gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg
    19681 gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag
    19741 caccgatcag tgcaatggcg ggtgcttatg gttctcatta cgaaatgctg caggattttg
    19801 atatggtggt cttagcaccg caagtcaatt cttactatga tgatatcaaa gaagacaccg
    19861 atcgcttagg aatcaagctg gttgcgacaa aaggagcaga atatatcgga ttaacgagag
    19921 atcctgaaaa agctgttcag tttattctga accaatttca ataaaggagg agccataatg
    19981 aaaatgggaa tttggttgcg taaagctgtt ttatttgcag ctgtgttagg ggctttgggg
    20041 gctgtttcaa aaactgttga agcatctgcc agcgaattta ttcgcggagc agatatttcg
    20101 atcctgtcag atatggaaaa atctggtgca aaatattatg aagacgatat ttcaaaagat
    20161 gccttagaga ttttaaaaaa taatggtgtc aactatgttc gactgcgttt gtggcaggat
    20221 ccttataata gtaatggaga aagttatgga gcagggacaa atgatctgga aacgacgatt
    20281 gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc tgctggattt tcactacagt
    20341 gatttttggg ttgatcctgg gaaacagaat cttcctaaag catggcaagg tctgaccttt
    20401 gaagaaatga atacggcatt gtacgactat acaaagaacg ttctgtcgga aatgaagcag
    20461 ctggacgttt atcctgatat ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg
    20521 ccttatggca agtcttgggg agaaggcggg ggagaatttg atcgattggc tgcttttttg
    20581 aagtctggta tccaagctgt tcgagacact caacctaaaa caactccagt aatgctccac
    20641 ttagctgatg gtggcgatac aggagcattt acttggtggt ttgatgaaat cacgagtcga
    20701 ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt attggcacgg ttcaatgaat
    20761 gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt
    20821 gaaacagctt atgggcatac aacagcgaat gccgatacta tgccaaatgc ttttggtgaa
    20881 gccgaagcag ctgcgggggg atatgaacca acaccgacag gacaagcaga gtatttgctg
    20941 gatcttgccg atcggattca ggcagtaccg aataatcgcg gggcgggctt cttctattgg
    21001 gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac aagcaggtat gaattatctc
    21061 agtgttcaat ccactatggg aaacgaatgg gacaaccaag cgatgtttga ttttgctgga
    21121 aacgccttgc caagcttgcg tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa
    21181 aatgcaagtt tcgaaagcga tggaccaacg accagtcctt ccaattggca aacctggttt
    21241 caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg gcactttttt tggggactac
    21301 aaactaactt tttggcagga tcaagcatac gaggcatcag cttatcaaaa tgttagtggc
    21361 ataagcaaag gaacttacac attgagtgcc tatgtaatgt ctgggggaga gtttgatact
    21421 aatcagttat atatcaaaaa ttatggtgga gcggaactaa atactgcggt tgttaaaaca
    21481 agtgaatgga caaaaattac aatcaaagac attccaatta caacaggtgt ttgtgaaatt
    21541 ggggtttata gcaaggcttc aggaaatgct tggacgaacg ttgattttgt ttcattggtg
    21601 aaacaataga ctgcggatcg tttcgattca agatcggagg ttcccttcag ccacatcgtt
    21661 agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc caaaaataac cagaaacgat
    21721 ttccagtcat ttttaccaaa acgagtaaag caccagactt catctacact ttgtattatg
    21781 ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga
    21841 taacataaag tttgtttaag aaagggtaac cttagaattc cgtcgtgaat atgatttgag
    21901 cacttagatg gatagaaaaa agttctcgga aaacagttcg tttttcgaga acttcttttt
    21961 atgataagaa tgaaacaaat taacttatat tatagtctct tttggtaggg aaccatctta
    22021 tttttaatga aaatcaagca ttttagctat tgaagaatgt tgatttatag ggattccccc
    22081 tccatattat gttggctctt tattcccact ctgttttcaa cgtaaagtgt ataaaacaga
    22141 atgcgatact atcaagggag ttaaaacaat atacagaaca gtaaaacact ataaataaaa
    22201 ataaaagtgc aataaaagtg caatttttgc acctaatcat actctgttta tatatgaaaa
    22261 ctagattctg aatccaatat ttggttaacg agaggcgttc gaatttgaac gtcttttata
    22321 tattactttc aaataaatat gaggtagaat actatctatt ttctttttaa cccaattgtt
    22381 ataatggaga gtgtaaaata ttttgtgtaa atagaaaaaa ggaagtccct tctgtagaat
    22441 agagttacca caacacattc acagaagaga ggacttccct atgaacgatt ttactacaga
    22501 aattctaaag actctagcga acaaaggcga tttgaatgaa ttattccgtg tccatttgga
    22561 gaaagcagtc aatacgcttc tcaaaacgga gttaacggct ttcctagatt acgaaaagta
    22621 tgatcgcatt ggttttaaca cgggtaattc tcgtaacggc tcctatgacc gtacggtcaa
    22681 gaccgagtat ggggaacttc atctccagat tccgcgcgac cgcaatggtg agttcaagca
    22741 acagactgtt cctgcttata gacggacgaa tgacacgtta gaggagaccg tcattcacct
    22801 cttccgaaaa ggtattacca tgtcggaaat cgcagacttg attgagaaaa tgtatgggca
    22861 ttactacacg ccccaaacca tgtccaatat aacaaaatca tttacagaag aggtaacggc
    22921 gtttaatggg cgggagcttc atgaccgtta tgctgctatt tatatggacg caacgtatat
    22981 tccgttaaag cggaaaaccg tcgccaagga agctattcat atcgcagttg gcattcgccc
    23041 ggacggatca aaggaagtat tgagctatgc gattgcaccg actgaatcca tcacgatttg
    23101 ggaggaaatt ttattggacc ttcaagagcg cggtttgaaa aatgtcctcc tgttcatcac
    23161 ggatggctta aaggggatgg taggagcgat tagtcggttc tatcccaaag ctcgttttca
    23221 acattgttgt gtacacgttt cccgtaatat cagtcataaa gtgcgtgtcg atgatcgtaa
    23281 ggaagtctgt gatgatttta aaatggtgta tcaagcctca tctaaagagg tggcgttgga
    23341 agcacgtggt acttttgcgg aaaaatggaa aaccagctat ccaaaagtgg ttgaatcgat
    23401 tctttcgaac gatcacttgc tcactttcta tgatttcccc ttggccatac gcaagagtat
    23461 ttattctacg aacttgattg aatcctttaa taaacaaatc aagaaataca gccaccgcaa
    23521 ggaacagttc caaaatgaag agtcgatgga acgtttctta gtctcgtctt ttgatactta
    23581 caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa caggccgaag gcgagctcga
    23641 acaaatgcta agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt
    23701 acacaaaatt attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa
    23761 cttactatat aatcatatta attaaatagt taggagatct gcttatgttt atatctactc
    23821 ttcaaattcg aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa
    23881 atactattat aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct
    23941 tattagatga tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg
    24001 gaattcaaaa tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca
    24061 gcgaagaaga atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa
    24121 caatcttaga agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta
    24181 ttagacctaa caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc
    24241 atcaatttaa cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat
    24301 ctaaatcaaa tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca
    24361 atgacaatat agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg
    24421 acatgtcaga tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc
    24481 ttagagaaat gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa
    24541 aaatatcatc agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta
    24601 ttaccagtgt tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca
    24661 taattggttc agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg
    24721 gttctttagc aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag
    24781 ggttaggaca tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc
    24841 gttcaagaga actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc
    24901 atattcaaaa aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca
    24961 tgaccacaca ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa
    25021 aatcgtttaa tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta
    25081 aagaaaagtt aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg
    25141 ttaaaagaaa tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag
    25201 aaatacttat tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa
    25261 taggtttgat taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg
    25321 atagaataca gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag
    25381 aacatcgatt gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat
    25441 cctccctttt taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa
    25501 ttgagttttt agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg
    25561 acgcattcaa agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat
    25621 atttagaaag aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg
    25681 caattgagtt aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg
    25741 ctattgcttt tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc
    25801 atacaatcga cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa
    25861 caagtgatac agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag
    25921 atgaggatgt tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg
    25981 aggtttcaaa atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa
    26041 agatgttgtg gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac
    26101 aagggtactt acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag
    26161 aataattgct atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt
    26221 acttagtata gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga
    26281 atttattatt tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat
    26341 tgatgattat actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct
    26401 caaaacgtat gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga
    26461 agttcagtat actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat
    26521 tgattttgat atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc
    26581 acgtaatatt gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga
    26641 attacaatac aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt
    26701 tgttggagat attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga
    26761 agaactagaa atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag
    26821 gtctaatcag agattagtag acttctactc taattttcag agttgtcctt acgagattga
    26881 aagcagaggg aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa
    26941 ggaggattta cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca
    27001 tgaaaaagat atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta
    27061 tttaaagaaa gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt
    27121 aaataatcat agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa
    27181 ggttcgaaga agaaagctaa ttgcaaacga tatttta
//
LOCUS       DACECS010000029        26647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.029, whole genome shotgun sequence.
ACCESSION   DACECS010000029 DACECS010000000
VERSION     DACECS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.029"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWO31_001405"
     CDS             complement(218..1015)
                     /locus_tag="IWO31_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0301829.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWO31_001406"
     CDS             complement(1012..2196)
                     /locus_tag="IWO31_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0301830.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWO31_001407"
     CDS             complement(2221..3468)
                     /locus_tag="IWO31_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0301831.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO31_001408"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO31_001408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0301832.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWO31_001409"
     CDS             5448..5762
                     /locus_tag="IWO31_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0301833.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWO31_001410"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWO31_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0301834.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWO31_001411"
     CDS             6447..7091
                     /locus_tag="IWO31_001411"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0301835.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWO31_001412"
     CDS             7106..7435
                     /locus_tag="IWO31_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301836.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWO31_001413"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWO31_001413"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0301837.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWO31_001414"
     CDS             8423..9247
                     /locus_tag="IWO31_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0301838.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWO31_001415"
     CDS             9240..9587
                     /locus_tag="IWO31_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0301839.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO31_001416"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO31_001416"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0301840.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWO31_001417"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWO31_001417"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0301841.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO31_001418"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO31_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0301842.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO31_001419"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO31_001419"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0301843.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWO31_001420"
     CDS             15244..16011
                     /locus_tag="IWO31_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301844.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWO31_001421"
     CDS             16001..18043
                     /locus_tag="IWO31_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0301845.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWO31_001422"
     CDS             18324..18473
                     /locus_tag="IWO31_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0301846.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWO31_001423"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWO31_001423"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0301847.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWO31_001424"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWO31_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0301848.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWO31_001425"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWO31_001425"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0301849.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IWO31_001426"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IWO31_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0301850.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IWO31_001427"
     CDS             22890..23972
                     /locus_tag="IWO31_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0301851.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IWO31_001428"
     CDS             23972..24775
                     /locus_tag="IWO31_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0301852.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IWO31_001429"
     CDS             24845..25585
                     /locus_tag="IWO31_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0301853.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IWO31_001430"
     CDS             25596..26600
                     /locus_tag="IWO31_001430"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0301854.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         8390 a         4730 c         5521 g         8006 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 tttttta
//
LOCUS       DACECS010000030        26615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.030, whole genome shotgun sequence.
ACCESSION   DACECS010000030 DACECS010000000
VERSION     DACECS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.030"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            257..1450
                     /locus_tag="IWO31_001431"
     CDS             257..1450
                     /locus_tag="IWO31_001431"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0301855.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1580..3052
                     /locus_tag="IWO31_001432"
     CDS             1580..3052
                     /locus_tag="IWO31_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0301856.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3135..3923
                     /locus_tag="IWO31_001433"
     CDS             3135..3923
                     /locus_tag="IWO31_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0301857.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3990..4658)
                     /locus_tag="IWO31_001434"
     CDS             complement(3990..4658)
                     /locus_tag="IWO31_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0301858.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4773..6485
                     /locus_tag="IWO31_001435"
     CDS             4773..6485
                     /locus_tag="IWO31_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301859.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6488..8257
                     /locus_tag="IWO31_001436"
     CDS             6488..8257
                     /locus_tag="IWO31_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301860.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8462..9028)
                     /locus_tag="IWO31_001437"
     CDS             complement(8462..9028)
                     /locus_tag="IWO31_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0301861.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9028..9984)
                     /locus_tag="IWO31_001438"
     CDS             complement(9028..9984)
                     /locus_tag="IWO31_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0301862.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9984..10640)
                     /locus_tag="IWO31_001439"
     CDS             complement(9984..10640)
                     /locus_tag="IWO31_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0301863.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11010..13028
                     /locus_tag="IWO31_001440"
     CDS             11010..13028
                     /locus_tag="IWO31_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0301864.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13455..15869
                     /locus_tag="IWO31_001441"
     CDS             13455..15869
                     /locus_tag="IWO31_001441"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0301865.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16217..17770
                     /locus_tag="IWO31_001442"
     CDS             16217..17770
                     /locus_tag="IWO31_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0301866.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18151..19935)
                     /locus_tag="IWO31_001443"
     CDS             complement(18151..19935)
                     /locus_tag="IWO31_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301867.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19950..21671)
                     /locus_tag="IWO31_001444"
     CDS             complement(19950..21671)
                     /locus_tag="IWO31_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301868.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21771..22532
                     /locus_tag="IWO31_001445"
     CDS             21771..22532
                     /locus_tag="IWO31_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0301869.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23499..23767
                     /locus_tag="IWO31_001446"
                     /pseudo
     CDS             23499..23767
                     /locus_tag="IWO31_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24439..>26615
                     /locus_tag="IWO31_001447"
     CDS             24439..>26615
                     /locus_tag="IWO31_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301870.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         8553 a         4659 c         5522 g         7881 t
ORIGIN      
        1 ctttttgagt atctttttct ttttatctga aagaatgatt ttaaaaagct tagcagagtt
       61 atctttatgg atgattgtgc taagcttttt tggacagttc taatgtaaaa tatttttata
      121 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
      181 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      241 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      301 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      361 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      421 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      481 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      541 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      601 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      661 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      721 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      781 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      841 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      901 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      961 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
     1021 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
     1081 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1141 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1201 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1261 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1321 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1381 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1441 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1501 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1561 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1621 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1681 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1741 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1801 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1861 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1921 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1981 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     2041 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     2101 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2161 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2221 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2281 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2341 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2401 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2461 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2521 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2581 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2641 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2701 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2761 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2821 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2881 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2941 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     3001 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     3061 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3121 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3181 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3241 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3301 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3361 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3421 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3481 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3541 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3601 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3661 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3721 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3781 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3841 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3901 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3961 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     4021 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     4081 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4141 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4201 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4261 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4321 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4381 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4441 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tatttgcaag
     4501 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4561 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4621 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4681 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4741 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4801 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4861 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4921 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4981 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     5041 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     5101 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5161 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5221 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5281 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5341 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5401 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5461 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5521 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5581 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5641 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5701 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5761 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5821 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5881 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5941 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     6001 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     6061 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6121 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6181 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6241 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6301 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6361 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6421 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6481 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6541 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6601 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6661 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6721 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6781 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6841 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6901 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6961 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     7021 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     7081 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7141 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7201 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7261 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7321 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7381 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7441 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7501 aaactgtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7561 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7621 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7681 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7741 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7801 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7861 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7921 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7981 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     8041 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     8101 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8161 tgatggggaa gtcattgaag aaggcagtca tcacgagtta ttagcagaaa atggatttta
     8221 tgcggaactt tatcataatc aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct
     8281 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga
     8341 cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc
     8401 tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta
     8461 tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc
     8521 gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat
     8581 ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag
     8641 gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga
     8701 ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt
     8761 gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt
     8821 ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa
     8881 aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat
     8941 tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat
     9001 ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt
     9061 tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt
     9121 tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg
     9181 gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg
     9241 acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat
     9301 cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac
     9361 attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac
     9421 acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc
     9481 tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa
     9541 atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg
     9601 tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc
     9661 tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg
     9721 ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag
     9781 agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc
     9841 gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt
     9901 gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt
     9961 tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt
    10021 tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtaa
    10081 ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt
    10141 actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa
    10201 gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta
    10261 gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa
    10321 aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa
    10381 aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg
    10441 atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga
    10501 taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg
    10561 aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga
    10621 aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt
    10681 tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc tttgaataac
    10741 caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta atgcaaatga
    10801 aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa cgtagacgta
    10861 ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata tgattacaaa
    10921 aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata attattaata
    10981 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
    11041 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
    11101 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
    11161 aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta
    11221 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
    11281 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
    11341 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
    11401 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
    11461 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
    11521 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
    11581 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
    11641 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
    11701 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
    11761 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
    11821 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
    11881 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
    11941 ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg
    12001 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
    12061 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
    12121 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
    12181 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
    12241 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
    12301 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
    12361 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
    12421 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
    12481 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
    12541 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
    12601 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
    12661 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
    12721 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
    12781 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
    12841 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
    12901 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
    12961 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
    13021 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
    13081 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
    13141 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
    13201 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
    13261 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
    13321 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
    13381 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
    13441 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
    13501 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
    13561 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
    13621 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
    13681 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
    13741 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
    13801 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
    13861 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
    13921 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
    13981 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
    14041 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
    14101 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
    14161 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
    14221 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
    14281 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
    14341 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
    14401 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
    14461 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
    14521 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
    14581 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
    14641 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
    14701 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
    14761 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
    14821 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
    14881 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
    14941 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
    15001 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
    15061 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
    15121 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
    15181 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
    15241 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
    15301 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
    15361 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
    15421 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
    15481 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
    15541 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
    15601 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
    15661 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
    15721 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
    15781 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
    15841 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
    15901 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
    15961 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
    16021 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
    16081 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
    16141 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
    16201 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
    16261 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
    16321 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
    16381 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
    16441 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
    16501 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
    16561 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
    16621 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
    16681 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
    16741 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
    16801 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
    16861 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
    16921 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
    16981 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
    17041 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
    17101 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
    17161 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
    17221 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
    17281 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
    17341 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
    17401 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
    17461 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
    17521 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
    17581 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
    17641 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
    17701 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
    17761 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
    17821 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
    17881 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
    17941 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
    18001 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
    18061 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
    18121 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
    18181 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
    18241 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
    18301 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
    18361 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
    18421 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
    18481 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
    18541 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
    18601 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
    18661 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
    18721 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
    18781 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
    18841 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
    18901 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
    18961 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
    19021 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
    19081 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
    19141 ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt
    19201 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
    19261 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
    19321 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
    19381 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
    19441 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
    19501 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
    19561 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
    19621 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
    19681 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
    19741 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
    19801 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
    19861 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
    19921 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
    19981 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
    20041 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
    20101 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
    20161 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
    20221 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
    20281 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
    20341 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
    20401 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
    20461 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
    20521 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
    20581 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
    20641 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
    20701 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
    20761 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
    20821 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
    20881 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
    20941 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    21001 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    21061 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    21121 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    21181 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    21241 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    21301 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    21361 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    21421 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    21481 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    21541 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct
    21601 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    21661 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    21721 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    21781 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    21841 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    21901 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    21961 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    22021 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    22081 gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    22141 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    22201 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    22261 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    22321 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    22381 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    22441 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    22501 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    22561 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    22621 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    22681 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    22741 aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa
    22801 aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat
    22861 aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt aataaggcaa
    22921 aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta ctgtgacttt
    22981 cctcagctat cggtgatact accgatagca tttttttgct tataaaataa ttataagtat
    23041 ttagagattt ttcttctaac attcttcaaa caagaaaaat cacaaaggtt attgttattt
    23101 ttatatattc tttttaagaa tgattgtttt tttatagaaa ttttaaaaaa taaaacacgt
    23161 tattgatgga tagttagttt gttatttggt aatattatag aagttgactg gtagtgattt
    23221 atttttcgtc gttttataaa agtgttattt tttggaaaaa ttcacaaata tgtaacgaat
    23281 gtttttctgg aatttgctgt gttaaaaagc agtatgacta atataaatac tattgtcatt
    23341 tatgcaattt tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg
    23401 aaaaatagtt gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat
    23461 tcgagagaat catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag
    23521 atagaattac caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc
    23581 gcttcggtta tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat
    23641 tgacattaca gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt
    23701 acaaggaaac cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac
    23761 aagttaagaa agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa
    23821 atgcaagaaa tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca
    23881 tccatgacga gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat
    23941 atggccaaat aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt
    24001 ttgatgctaa gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggatt
    24061 cactgatgaa attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg
    24121 aaaagtataa aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt
    24181 gtgcaaggca ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa
    24241 ctaaaagtca tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt
    24301 tgtttacagc ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag
    24361 ccattttgag ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa
    24421 ttataagggg gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt
    24481 atgcttattg tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa
    24541 cagccagagc gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag
    24601 ttccagttgc gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt
    24661 gaggatttga ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat
    24721 tcttcccagc attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat
    24781 acggtctcaa tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca
    24841 gaagaaagca caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat
    24901 gaagccagat catctgagcc atctataacg caggaatctc agattgaaag acaagaggag
    24961 ctcttgaatt atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca
    25021 caaacgttaa agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag
    25081 atggtcgtac gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc
    25141 gctgatgatt cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt
    25201 ccatttacct ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc
    25261 acgtttgaag ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg
    25321 agtcttactt atgctgagcg agcagcaaac acggaatttt caattacgcc tattccttca
    25381 tcaagaccca ttttttcgaa attatactat ggctctagag aaatcgacag ccaaggaaat
    25441 cttgggaatc tgctgattga cgggcaagac gtgaccaaca gtaataaatt tattttgcag
    25501 gtaaattatg aaggatcacg aaaagacggt gtagtggtta ccgatgtact accgcaagga
    25561 acaactttgg caccgaatcc gactacatgg agttccactg gaaatagaga acctatttca
    25621 ggtggggttc ggatcgcaga agtaacgaat ccaggtgaag caaacgttag aactcattat
    25681 gtttccgagg aatttgtgga caaagtatct tttaataagg aaacgaatac acttgttgtg
    25741 gactttggta atctagcaga gggtcaatcg tattttattg aatatgctct tgaagtgacc
    25801 gatgtcgact tgttcaatga aggaagacaa gaggatgggt tttctcggaa tcgagcgagt
    25861 atgacctaca ataatcaggt aatgagccga gactaccgct tgagaatccc tgaaagtaat
    25921 cttccggcaa tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat
    25981 gaactaacgt atactctgca tgtctcgatg ttttctggaa caagaaatac gcttgttatt
    26041 gaagatgttt tggaagaagg catggaattt gtgtcatttc tatcagatat tccctctttc
    26101 attgatgccg aagtcaataa tggaatagtt cgtttcagtg cgaataatac gattggtggt
    26161 ccgacaagtt ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa
    26221 attttaaact ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag tactgtaaga
    26281 acaaggaaaa tcgatgggcg tattaaagta attaaaactg atgagaatgg ccaaaggctg
    26341 gcaggagcaa aatttgaaat tttatcaagc gattcacaag tgatacaaga aggtataacg
    26401 gcagaaaacg gagaattttt aagtgaaccg cttcctgtag gtagctatca agtccgagaa
    26461 acagaagcac cggaagggta tcttttagat gcgacggtac atgatgtttt tataaatgaa
    26521 tactccatag aagaagtgac gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa
    26581 ctaattaaac atgctgaagg cagtggtgaa gtcct
//
LOCUS       DACECS010000031        26305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.031, whole genome shotgun sequence.
ACCESSION   DACECS010000031 DACECS010000000
VERSION     DACECS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.031"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            186..1124
                     /locus_tag="IWO31_001448"
     CDS             186..1124
                     /locus_tag="IWO31_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0301871.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            1147..1827
                     /locus_tag="IWO31_001449"
     CDS             1147..1827
                     /locus_tag="IWO31_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301872.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            1800..2624
                     /locus_tag="IWO31_001450"
     CDS             1800..2624
                     /locus_tag="IWO31_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0301873.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            2791..3609
                     /gene="purR"
                     /locus_tag="IWO31_001451"
     CDS             2791..3609
                     /gene="purR"
                     /locus_tag="IWO31_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0301874.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            4112..5485
                     /gene="glmU"
                     /locus_tag="IWO31_001452"
     CDS             4112..5485
                     /gene="glmU"
                     /locus_tag="IWO31_001452"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0301875.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            5579..6550
                     /locus_tag="IWO31_001453"
     CDS             5579..6550
                     /locus_tag="IWO31_001453"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0301876.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      6556..6658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6724..7293
                     /gene="thiT"
                     /locus_tag="IWO31_001454"
     CDS             6724..7293
                     /gene="thiT"
                     /locus_tag="IWO31_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0301877.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(7374..8147)
                     /locus_tag="IWO31_001455"
     CDS             complement(7374..8147)
                     /locus_tag="IWO31_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301878.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            8414..10114
                     /locus_tag="IWO31_001456"
     CDS             8414..10114
                     /locus_tag="IWO31_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0301879.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            10084..10302
                     /locus_tag="IWO31_001457"
     CDS             10084..10302
                     /locus_tag="IWO31_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301880.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            10322..11212
                     /locus_tag="IWO31_001458"
     CDS             10322..11212
                     /locus_tag="IWO31_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0301881.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            11230..11514
                     /locus_tag="IWO31_001459"
     CDS             11230..11514
                     /locus_tag="IWO31_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301882.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            12001..12615
                     /gene="gmk"
                     /locus_tag="IWO31_001460"
     CDS             12001..12615
                     /gene="gmk"
                     /locus_tag="IWO31_001460"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0301883.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            12620..12925
                     /locus_tag="IWO31_001461"
     CDS             12620..12925
                     /locus_tag="IWO31_001461"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0301884.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            13178..15586
                     /gene="priA"
                     /locus_tag="IWO31_001462"
     CDS             13178..15586
                     /gene="priA"
                     /locus_tag="IWO31_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0301885.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            15601..16092
                     /gene="def"
                     /locus_tag="IWO31_001463"
     CDS             15601..16092
                     /gene="def"
                     /locus_tag="IWO31_001463"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0301886.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            16085..17023
                     /locus_tag="IWO31_001464"
     CDS             16085..17023
                     /locus_tag="IWO31_001464"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0301887.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            17023..18381
                     /gene="rsmB"
                     /locus_tag="IWO31_001465"
     CDS             17023..18381
                     /gene="rsmB"
                     /locus_tag="IWO31_001465"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0301888.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            18394..19134
                     /locus_tag="IWO31_001466"
     CDS             18394..19134
                     /locus_tag="IWO31_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0301889.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            19131..21200
                     /gene="pknB"
                     /locus_tag="IWO31_001467"
     CDS             19131..21200
                     /gene="pknB"
                     /locus_tag="IWO31_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0301890.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            21365..22264
                     /gene="rsgA"
                     /locus_tag="IWO31_001468"
     CDS             21365..22264
                     /gene="rsgA"
                     /locus_tag="IWO31_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0301891.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            22277..22927
                     /locus_tag="IWO31_001469"
     CDS             22277..22927
                     /locus_tag="IWO31_001469"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0301892.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            22928..23566
                     /locus_tag="IWO31_001470"
     CDS             22928..23566
                     /locus_tag="IWO31_001470"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0301893.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            23721..24575
                     /gene="mreC"
                     /locus_tag="IWO31_001471"
     CDS             23721..24575
                     /gene="mreC"
                     /locus_tag="IWO31_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0301894.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            24579..25088
                     /gene="mreD"
                     /locus_tag="IWO31_001472"
     CDS             24579..25088
                     /gene="mreD"
                     /locus_tag="IWO31_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0301895.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            25343..>26305
                     /locus_tag="IWO31_001473"
     CDS             25343..>26305
                     /locus_tag="IWO31_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301896.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATE"
BASE COUNT         8794 a         4706 c         5658 g         7147 t
ORIGIN      
        1 ttttttattg tagtgaaatg gctagatatg agcagcaact catccatttt acgatttaat
       61 caagaatata catgcttaaa tcagaaattg atggatttct attgactgtg tttttttatt
      121 ttgatacatt agttcacgtt aataaatcgt aaaattccga tttgttcatt gaaaggagca
      181 tataaatgaa aaaatatctt ttgacagcta cggtaatgat aggtgcttta ctgtttgcag
      241 catgtggaaa tacgaacaag gaagcagaca aaaaagagga tttgacgatc gttacgactt
      301 tttatccgat gtatgatttt acaaaagaga tcgtagggga tgaaggaaac gtgaaacttt
      361 tgatccctgc tggaacggag ccgcatgatt tcgaaccaag tgcaaaagaa agagcagaaa
      421 tttccgatgc agatgttttc gtgtataata gttcagacat ggaatttttt gtggattcgt
      481 taaaggattc agtggatagc aaacagacct tgatgatcga agcagctaaa ggaatcgacc
      541 gtttagaatc gcaagaagca gacgaacatg aagaatcaga agaagggcat ggtcattcac
      601 atgaatacga tccacatgtt tggctcgatc cggtattagc tataaaagaa gtcagaacga
      661 ttgctgaaga attaggcgaa aaatatccag ataaaaagga aacttttacg aagaatgccg
      721 atgcttatat caaaaagctt gaagcactgg atcaaaaata ctccgatgag ctaaaagatg
      781 caacaaaccg cacatttgtc actcagcatg ctgcttttgc ttatttagcg aatcaatacg
      841 acttaaaaca agtagcgatt tcgggcgttt caccagatca ggaacccaca ccaagtcgct
      901 tagctgagct aaaagaattt gtgaagaaaa acaacatcaa agtgatttat tttgaagaaa
      961 atgcctcttc gaaggtagct gagacattgt cgaatgaaac aggagtgaag ctagaagtgt
     1021 tgaatccttt agaaagcttg actaatgaac aaatcaaagc aggcgaaaat tatatttctg
     1081 tcatggaaaa aaatctgaaa gctttaaaag aaagtatcaa ttaagtttta gaatggaggt
     1141 tgtaaggtgc gttatatcga aatatctgat ttgacctttt actatgatga tgaacctgta
     1201 ctagaagatg tctcttatca tgtagatgcg ggtgaatttg tcattttaac tggagaaaat
     1261 ggagcagcca agtcaacctt ggtgaaagca tcgttaggat tactgaaacc ttcaaaagga
     1321 atgataaaga ttgctgagaa aaatcaagaa ggaaaaaaat taagtatcgg ttatattcct
     1381 caacaagttg catctttcaa tgcaggtttt cctagtacgg tgatcgaatt agtccgttca
     1441 ggaagatatc caagaggccg ctggttcaag cgtctctctg aaaaagatca tcaacatgta
     1501 cagcgtgctt tagaagctgt agggatgtgg gatatgcgaa atcggcgaat aggtgaattg
     1561 tctggcggac aaaagcagcg aatcagttta gcgcgtattt ttgctactga ccctgactta
     1621 tttgttcttg atgaaccaac aacaggtatg gatgaagaat ccagaaatga attttatcgt
     1681 cttctccgac atagtgcgca tgtccatggg aaatcagttc taatgatcac ccatgatcat
     1741 gaagatatca aacagtatgc ggacagacag atccgactgg tgagaaagga ggattctcaa
     1801 tggcgttgct ttcatatgaa ttcatgagaa gagcgtttct tgccgctatc ttcattgccg
     1861 gaatcgcccc gatgctaggt gtgtttttag tgatcagaag acaatcgctg atggctgata
     1921 cgctttctca tgtctcttta gcaggggtag cgttaggatt ctttttgaac cttaatccta
     1981 cacttacgac catgctagtc gttgtattag cagctgtgat attggagtat ctgcgaacga
     2041 tctaccgaac ctattcagaa atctccattg ccattttaat ggctggtggt ttggcgctag
     2101 cgttagtatt gatgaatttg agcggaggga attcagcaac aagtatccaa tcctatctgt
     2161 ttggttcgat cgtgaccatt acatgggagc aagtcatctt tttaggaatc ttattcgttg
     2221 ccatcggtat attgttcttt ttattcaaac ggccgatgta tgttctgact tttgatgaag
     2281 ataccgccca cgtggatggg ctacccgttc atttgatgtc gatgatgttc aatgtgatta
     2341 ctggtgtggc tattgctgtg atgatcccga ttgccggtgc acttttgata tccgcaatca
     2401 tggttcttcc cgcagcaatc ggtatgcgat taggcaaaag cttcaatgtc gtgattgcta
     2461 tcagtgtttg tgtaggattt ctagggatgc tgtcaggact aactagctcg ttttatctag
     2521 atacacctcc aggagcaaca atcacattag tctttattgc tttgttcctg attgtaaata
     2581 tcatcaaacg aatattggtt gccttcagaa gaaaaaataa ctaaattata aagaaaagaa
     2641 gccttataaa aacgaaaaat tttttatagg cttcttttta ttttaataaa taccgaacta
     2701 ttttcttatt ttttcataaa atgctcgaat ttttgaagaa aaacttatat aatgtaaaac
     2761 gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt
     2821 gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc
     2881 gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca
     2941 tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg
     3001 ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt
     3061 ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga
     3121 gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa
     3181 atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat
     3241 tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca
     3301 gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag
     3361 agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga
     3421 acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact
     3481 gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt
     3541 tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt
     3601 tcagaataag aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa
     3661 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
     3721 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
     3781 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
     3841 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
     3901 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
     3961 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
     4021 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
     4081 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
     4141 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
     4201 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
     4261 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
     4321 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
     4381 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
     4441 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
     4501 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
     4561 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
     4621 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
     4681 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
     4741 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
     4801 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
     4861 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
     4921 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
     4981 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
     5041 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
     5101 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
     5161 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
     5221 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
     5281 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
     5341 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
     5401 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
     5461 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
     5521 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
     5581 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
     5641 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
     5701 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
     5761 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
     5821 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
     5881 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
     5941 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
     6001 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
     6061 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
     6121 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
     6181 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
     6241 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
     6301 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
     6361 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
     6421 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
     6481 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
     6541 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
     6601 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
     6661 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
     6721 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
     6781 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
     6841 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
     6901 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
     6961 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
     7021 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
     7081 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
     7141 tatgcactat gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg
     7201 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
     7261 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
     7321 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
     7381 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
     7441 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
     7501 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
     7561 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
     7621 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
     7681 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
     7741 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
     7801 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
     7861 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
     7921 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
     7981 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
     8041 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
     8101 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
     8161 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
     8221 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
     8281 ttttgtgatt tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa
     8341 tctacaaagt cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa
     8401 aaaggagagc gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc
     8461 gcctatccag caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga
     8521 cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac
     8581 cgtctatttt actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga
     8641 aggtgaaact tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt
     8701 tattaaagaa aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat
     8761 ttttcataaa aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac
     8821 agcacttgtt ttaggtggag gaggagcgca cggtgcttat cagattggtg tgtggcaggc
     8881 gctgaaggaa catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa
     8941 cggcgcattg attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc
     9001 gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt
     9061 ctatcgagaa agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc
     9121 tccattggaa aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa
     9181 aatacgttta tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat
     9241 ccaacagaca aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc
     9301 cgctatggct tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa
     9361 aatacctatt gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca
     9421 agggccaggc ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca
     9481 aacattgtgg acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt
     9541 acaattaggc tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac
     9601 atttcattct gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa
     9661 agaaattcag ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa
     9721 tttatataag aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc
     9781 aaaaaaacat tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat
     9841 ctgccaacaa gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag
     9901 tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa
     9961 aactgtttat tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt
    10021 gcttaagcaa ccagttgaca cattactgat cctatattta tattatctca aggaggaaca
    10081 accatggcac aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac
    10141 aacaaggggt ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc
    10201 gatcttcgag attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac
    10261 gaagagtttg aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg
    10321 aatgaaaagt atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga
    10381 tattgaaata aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat
    10441 cctgaacttt gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg
    10501 aatcgaagta tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa
    10561 ctgggaattg attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg
    10621 tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc ttcgtgctgg acgaaaactt
    10681 cgtaacggta gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga
    10741 tacattgaaa agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt
    10801 agctttagtg ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag
    10861 ttttgtagaa atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt
    10921 tcttggagcg aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg
    10981 cggagatatt cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact
    11041 tatcgaagca caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg
    11101 ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca
    11161 gtctaagacg atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag
    11221 gtagagataa tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt
    11281 aaacgggata tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac
    11341 tataatgaga acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg
    11401 aaatttattg ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct
    11461 cgaaaagatg agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga
    11521 tgctgaacaa agaaatacaa taaataaaca gaattggatt tcttcgctta gcttctttgc
    11581 tctaggaata catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa
    11641 taagtgatgt tcaaaaacca ttttctcggt attaagccaa atttatgcaa aaatgtgaga
    11701 aactattttc acaaaatttt tcttcatact cgaaactaat cggcttcttc acgacctctt
    11761 atacacggcg ttcaaaagct tgcatctgcg ataataaccc caaccaaacg aaaaacatga
    11821 gaagctgttt atgtttggct gttcttatta cttgatgcag gacagctttt ttacgacctc
    11881 ttggtacagg gtagcttttt taccaatttc tagctataat taaagataac cttgaaagtt
    11941 gcagaaaaaa agtgcaaaat aaacgataag tacaaaaaga ctagttgaaa ggacgtcgtc
    12001 atgtcagagc gtggattatt aatcgtgttg tctggtccat ccggtgtagg aaaaggcaca
    12061 gtacgaaaag caatttttga gagtgaggat aatgattttc agtattcgat ctcaatgact
    12121 acccgtaaaa tgcgtgaagg ggaggtcgaa ggcgttgatt atttcttccg aagcaaagaa
    12181 gagtttgaat caatgatcga agctggagag atgctggaat acgcagaata tgtcgggaat
    12241 tattatggaa ctcctttgtc atatgtcaac aagacattgg atgaaggaaa agatgttttc
    12301 ttggagattg aagtccaagg agcaaaacaa gtcaaagaaa aagtgcccga tggtgtattc
    12361 atctttttga ctccgccaga tttagcggaa ttaaaatcac ggatcgtagg ccgaggaaca
    12421 gatgcagatg aagtaatcga aaaacgaatg aaagttgcaa aagaagaaat cgaaatgatg
    12481 gctctttacg actatgctgt tgtaaatgat gaagtgccgc tagcagttga acgaatcaaa
    12541 aacattattg ccagtgaaca ttttcgagta gatcgcgtaa tcggtaaata tattaaaatg
    12601 ttaaaggaga tgtagtctta tgatgttaaa accttcaatt gactcactat tagatcgtgt
    12661 aaattcaaaa tattctctag tgattttagc aagtaaacgc gcgcatgaat tggatgcaag
    12721 tgcacagcct actctagatt cttttgattc agtaaaaagc gtcggtcaag ctttagaaga
    12781 aatcgatgca ggaaatgtag tcaatgatcc acatcctgaa ttaaaacgtg aacgtttgaa
    12841 gatggaagaa gaggaacgtc aagcacaaaa agagcgtgaa caacacgaat tggaatcacg
    12901 tatccgtgaa gagcaaaaaa tgtaataaag aaaaatagag cgaacatcta aaaagaaaag
    12961 caaacccagc tgtttccttt tcaaacattc gccacttatt gtctggtaga aaaggctgtg
    13021 tctgaacttt ttgacacagt tctttttttt cttatctgct gttaattttt tgttaggatt
    13081 tggcagaaga taagacaaat ccatcgatct ctagtaaaat aggcaaggaa ctttttgtcg
    13141 cttgataaga aaagacaaaa aaaggaggaa acgaaccatg acattaacag ctgaagtcat
    13201 tgtggacgta cctactatgc agaccgatca gccattcact tatttggttc cctctgaaat
    13261 ggaaacagct ttgcaagtcg ggatgcgagt agaagtccct tttggtaatg gcaatcgtca
    13321 tgtccaagga tttgttatgg ctatccaaaa atcagaagaa tcagctaatc catctttgaa
    13381 ggcagttatt cgtttgctag accttgctcc ggtggtcaat gaagaattac tttcattagc
    13441 tgattatatg aaaaaaatta cttacgcatt caaaatcact tgtctacaaa cgatgcttcc
    13501 gagtgtgatg aaagcagaat atgataaatt gatctatcct ttggcagaca caccagaagt
    13561 tcaggtattg tttgatcagc gagaagtgat ttcctggaag gaagcagaag aagaaggcag
    13621 cctttctcaa ttgattcgct ggcgccaaga gcaattagta gatatcaaat atgaagtcca
    13681 tacacgaaat aaagtaaaaa cgattcgttt ggtccgttct ttgttaactg aaaagcaaat
    13741 cgaagaagaa tgggcaaaat tacggcaaaa tgccaagaaa caaaaagaac ttcttttgtg
    13801 tttatctgaa ctgagccaag aagaaccaat tgcttatttc aaaaataaag gaatcagtac
    13861 tgctgtcttg aatcaaggaa aagaaaaggg ctggctggag tttatagaat cagaacgtta
    13921 tcgcgaccct tataaagacc gagtatttga ccaaacgacg gcattggaac tgaatgcaga
    13981 acaaaaaaat gctgtcgaac aaatcatcac agcaggtcag cagcaaaaag atcaggtctt
    14041 tttgctggag ggaatcactg gcagcgggaa aacagaagta tatcttcagg caatcgctga
    14101 tgttttgtca gaaaataaaa cggctattat gttagtgcct gaaatcgcat tgactccgca
    14161 aatggtcgag cgattcaaag gacgcttcgg agaatcagtt gcggtcttac atagcggact
    14221 ttcccaaggt gaaaaatatg atgaatggcg caagattgag cgggaagaag cgcaagtagt
    14281 agtaggcgca cgatctgcta tttttgcccc gctgaaaaat atcgggctga tcattatcga
    14341 tgaagaacat gaatcaagtt acaaacaaga tgagacaccg cgttatcatg cgagagatct
    14401 agcaatttgg cgcagtaaat atcatcattg cccaatagtt ctaggaagcg cgacgccctc
    14461 tttagaatca agagcacgag ctcaaaaagg cgtttatcag ttattgcagt taaaccaccg
    14521 agccaaagca gctgcacagc ttccagctat tgagatcgta gatatgcgtg aagagtttca
    14581 gaatcatcgg acctctactt tttctgctaa tttgcaggaa aaaatacaaa atcgattaga
    14641 caaaaaagaa caaactgtcc tactgctcaa tcgtcgagga tattcgtctt ttgtgatgtg
    14701 tagggactgt ggatttgttc ttccatgtcc taattgcgat atctctctta cactgcatat
    14761 ggatacacgt tcaatgcgct gccattattg cggacatgaa gaacctattc ccaatcgctg
    14821 tcctaattgt ggcggcaata aaatccgcta ctatggaaca ggtactcaaa aagtagaaga
    14881 ggaactccga gaactttatc cgcaagctag gattttgcgt atggatgtag ataccactcg
    14941 aagaaaaggg gcacacgaac agattttaca aaaattcggt gcaaaagaag cagatatatt
    15001 attagggaca caaatgattg ctaaaggttt ggactttcca gaagtgacat tagttggggt
    15061 gttgaatgct gatacctccc tgaatttacc ggattttcgt tctagtgagc atacattcca
    15121 attgcttacc caagtttcag gacgagcagg gcgagcggaa aaagcaggcg aagtcgtgat
    15181 ccaaacattc aatcctcaac attacgccat agaattggct aaaaaacaaa attatgagca
    15241 gttttatcag caggaaatgc atgtacggca tcgtggaggc tatcctcctt attactttac
    15301 tgtgaaaata accgccagcc atcctgaaga acaagtagcg gcaaagaaaa tctttcagat
    15361 tgccaatcag ctaaaagaag tgttaagccc tcaaagcttg ctattagggc caacgcccag
    15421 catgattttg agagtgaaaa accgatatta ttatcaacta atcatcaaat ataaacatga
    15481 accgaatctt cctcatgttt tggatgaaat tttgaatgga agccagaaag aacagcgaca
    15541 aggtcttttt gtggcaattg acaacgaacc attaaacttt atttaagaaa gggccaaatt
    15601 atgcgctatc ctatattgat ccaccctaat gataaattaa aaagaactgc tcagccaatc
    15661 gatgtaatca cggacgagac aatcgcttta ttagacaatt tatatgagac aatgattgcg
    15721 aatgacggaa tagggatcgc tgctcctcaa gtaggacaaa acaagagaat tgctgtgatc
    15781 gaagtagatg aaggcgagaa atttgaactg attaacccag aaatcatcga agcaaaaggt
    15841 gaaagccttg acgtagaagg ttgtctcagt attccgcatg tctatggaac ggtcaagcga
    15901 gcagatgaag tgaccgtgcg ttattatgat cgagacggag aagagataga agtgacggct
    15961 tttggctatt tagcccgtgc cttccagcat gaaatcgatc acttagacgg tatcttgttc
    16021 attgagaaaa tgatacagca gatcccggaa gaagaacttg aagaatatat ggaggaacat
    16081 cttgatgact aagctagtat ttatgggcac acctgctttt tctgtgccga ttcttgaagg
    16141 gctgatagaa tcaggttacg agatccaggc agtggtaaca cagcccgacc gtccagttgg
    16201 gcggaaacga gtaattacgc cgacaccggt aaaaaaagcg gctctgaagc acggtatccg
    16261 tgtgctgcag ccagagaaaa tctccggttc gccagaaatg gaagaaatca ttgaattggt
    16321 gcctgatgtg atcgtcactg cggcatttgg ccaattcttg cctgaaaagt tattgcaagt
    16381 accaaaatta ggggcaatca atgtccatgc ttctttactt cctaaatatc ggggaggtgc
    16441 gcctgttcat tatgccatca tgaatgggga gaaagaaact ggtgtaacga tcatggaaat
    16501 gatcaaaaaa atggatgcag gcggaatcta tgcgcaagaa agcataccaa tcactaaaca
    16561 agatgatgta gggacgatgt ttgaaaagct tagcctttta ggaagaaagc tattgctaga
    16621 aacattgccc aatattttgg atggacaaaa accagtacct caagacgagt cagaagcaac
    16681 tttttcgcca aacatcacac gggaacaaga agcaatcgat tggcataaaa ctgcagaaga
    16741 aattgacaac caagtacgcg gaatgcgtcc ttggccaatt gcattcacga cctatgaggg
    16801 catacgctgg aaattattat cggttgaagt attggcagaa actacagaca aagcacctgg
    16861 tacgatcatc cggaaagaca aaaaaaatct atggattgct tgcggtaaac agaccgtctt
    16921 agccatcaat gaattacagc cagctggtaa aggaaaacag gccgtacatg atttccttaa
    16981 cggaagcggt cagcaagttc agattggtca gcaggtgaaa taatgaaaaa aatccctaaa
    17041 aaaatgaaaa aatcagtacg atatatggca ttactgacat tagagcgagt gaacaagggt
    17101 ggcgcctact cgaatctttt gttgaatgag atgatcaaac aaagcgagct aaatcaaaaa
    17161 gatgtgggtt tatttacaga attgatctat ggtacgatta gccatcagcg actattggaa
    17221 ttttacgttg caccattgat cgctaaagca aaaaaagtgg atgactgggt caagatgctt
    17281 ctttatcttt ctgtatacca attagaattt ttagataaag tccctgcaca tgctatttta
    17341 aacgaggcgg tcgaaatcgc aaaagtaaaa ggaaatcctg ggacagggaa atttgtaaat
    17401 ggtgtcttga ggaattatca gcgtcaagga gcaccagatc tgcaaggcat tgctgatcct
    17461 atcgaaagac tttctgtcaa aatcagcctg cctctatgga tgacgaaacg actggtggat
    17521 caaatcggat acgaagaaac tgaaaagctc ggtttatcgc tatatcagcc tagccatgct
    17581 agtgcccgag tagatacacg ccgactttcc agagatcagg ccatcactgt tttgcaagaa
    17641 gaagagatca cagctgaaaa aagccaagtc agtccatacg gtatagtagc gaaaaaaggg
    17701 catttagctt ctagctcatt gttccacgat ggcgtcatga cgatccaaga tgaaagttcg
    17761 atgcttgtag cgccgtctat gcaaatcgag cctgaacatc atgtcttaga tgcctgtgca
    17821 gcacccggag gtaagacgac gcatattgct tcttttttag atccgtcatg cggtggagaa
    17881 gtggtcgctt tagatgtaca cgaacacaaa gtcaaactca ttgaagataa tgcagcacgt
    17941 ctaggtgtct cttctgctgt ctacgctcaa aagatggatg ctagagaagt cagtaaacag
    18001 ttcgaagcag agcagtttga tcggatttta gtagacgcgc cttgttcagg tcttgggctg
    18061 cttcggcgaa aaccagatat tcgttataaa aaacagccgg atgaattagc gaatttacca
    18121 aaaatccaat tggaaatttt agaaagctgc gcaccaactt taaagtcttc cggtattctg
    18181 acttatagca cttgtacgat cttaaaagaa gaaaaccaag acgttgtttc tgctttcttg
    18241 gaaagacacc ctgattttga gaggattgat gttttgacca atcctatgct acaatcatca
    18301 atcgaagaga aaatgttgac tttgtatccg catcagtttt acacagatgg atttttcatt
    18361 tgttgtttgc gcaaaaagta atgaggtgaa aaaatgcaga ttgaatatca atcagatgta
    18421 ggaagacgac gtaacacaaa tcaagactat gccagtgtat ttaccagccg ttcaggtctc
    18481 aagcttgctg tattagcaga cgggatgggt ggtcatcgtg ccggagacat tgccagtcag
    18541 atggcggtaa cgaatctagg cgaagcttgg gaaaaacaag aagctgttga cgatgaaaaa
    18601 atcgcccaat ggttcattca atcgattcaa gaagaaaata tgctgattta tcagcgtgga
    18661 caagaacagc cggaatataa cggtatggga acaacaatcg tagcggccgc tctgtcagat
    18721 gactggttca cgattgccaa tgttggggat agcagagctt atttgattcg tgaagataaa
    18781 atcatgcaat tgacggagga tcattcttta gtcaatgaac tagtcaaatc aggagaaatc
    18841 agcgaagaaa tggcagtgaa ccatccaagg aaaaatattt taacacgttc tgtcggcatg
    18901 cctggaacag tcgaagtaga tgtttctact catctttggc aactaaaaga tcgactgctg
    18961 ttatgttcag atggcttgac gaacatgctg tctgaagaag aaataggaac gatcatcaat
    19021 caagaaggcc cattgtctca taaagtaact gagctgattg atcgtgccaa tgaagcaggc
    19081 ggagcggata atatcaccgt tcttcttatc gaatataagg aggacagtgt atgatcgagt
    19141 tgggcaaaaa attaaatggc cgttatcaaa tcactggaaa tatcggtagc ggtggtatgg
    19201 caaatgtatt tttggcgcac gatttaatct tggatcgtga tgtagcagtt aaagtcttac
    19261 gatttgactt tcaaaatgac cagactgcga ttcgacgatt tcagagagaa gctttagcag
    19321 caacagaact cgttcatcca aacattgtta gtgtttacga tgttggtgaa gaagacaaca
    19381 tgcagtatct ggtgatggaa tacgtcaaag gaatggactt gaaacgatat atccaaacac
    19441 attatcctgt tccttatgaa acagcagtaa atattatgca gcagatcctt tctgctattt
    19501 ctttagctca taaccatcaa atcatccacc gagatttgaa accccaaaac gtactgatcg
    19561 ataatgaagg tgttgtaaaa atcacggatt tcgggattgc tatcgcttta tctgaaacat
    19621 cgatcacgca gacgaatacg atgctaggat ctgttcatta tctgtcccct gagcaggcca
    19681 gaggaagtat ggcaacgaag cagtcagata tttatgcttt agggatcatc ctttatgaga
    19741 tgttgacggg tagcgtaccg tttgatggtg aatctgctgt aacaattgct ttaaaacatt
    19801 ttcaagatga tcttccatct ataaaagcac ttgatccaaa tgtgccacaa gcactggaaa
    19861 acgtgatttt acgggcaacg gctaaagaac ctgcagaccg ttataagtca gccgaggaaa
    19921 tgtctgatga tctttctaca gtattatcac cagctcgtgc aaacgaagaa aaatggcagc
    19981 ctcatgtgat ggataatgaa acaaaagtta tcacacccct tacggaagat gcaccaatgc
    20041 ctgattcatt caaatctatg cctctgccaa aagacaagcc agaaaacgaa accttaccag
    20101 aggaggaaga agttctgcct gatcctaaaa agaaaggaca gaaaaaatgg tggctgatcc
    20161 tattagctat tcttgctgtt ttaggaatcg gaacagctgt attctttgct tctggcgggc
    20221 gaggagaagt tgttgttcct gatgtttctg atctttctga agcttctgct agagaaacac
    20281 tcgcaaaagc gaaattagaa gtagcaacaa aaacagaaga aattgcagat gacaaaatcg
    20341 aagaaggtaa tgtagtgaag acagacccgg cagcgggaac gacagtcaag caaaaacgcg
    20401 aagtcactct gtatattagt agcggcacga aaaaaatcaa attagatgat tacagtggta
    20461 aaagttatga agatgccgaa gcaagtttga aagaattagg gttttctgct gacttgattc
    20521 aaaaagaaga ggaattcagt tcagaggtag aaagcggaat gatcatttcg caatctccat
    20581 cagaagatac agaagtcgat cctaaaacag acacgattac tttcacagtc agcaaaggac
    20641 cagaaacagt gactttgagt agtcatgtag gagaaaacta cgaaactgtt cgtaattggc
    20701 ttgttacaca aggattcaat atcaataata ttagtgctac ttatgactat agcgacactg
    20761 taccatcagg ttcgatcatc agtcaggatc cagcctcggg tgaagttgta gcagaagaaa
    20821 cttacatcaa ttttgtcgta agccaaggaa cagagcctaa attgaatgat atctccggct
    20881 atacaaaaag cgaagcacaa agttatttag ctagtgtcgg tgcagaatat atcgggcatg
    20941 agacttccga atattcaaac acggtggata aagataaagt gattcgtaca gtaccagggg
    21001 caggaacgac aattaccaaa ggaatggttg tgaacgtaat ttactctaaa ggaccagatc
    21061 cagcgtcttc tagttctgaa aaagactctt cagacagttc tagcagctca agtagctcca
    21121 gtagcacaag caactcttca aaagaagaat cttccagctc cacaagttca tcagctagca
    21181 cctcagaaag ttcaaaataa gtgctgaata aaatatatca gaagggaatg cgacattgat
    21241 ttgttgcatt ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt
    21301 tgagaagttt gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac
    21361 ggaggtggca cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata
    21421 cgcaaacgga gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac
    21481 gccgcttgtc ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct
    21541 ggaaatcttg ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg
    21601 aatcattgtc acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct
    21661 agttaccttg gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc
    21721 agacgaccca gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt
    21781 gattgtaccc aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct
    21841 atcggtcttt atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc
    21901 ggaactgaat ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac
    21961 acgtcatgtg gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag
    22021 ctcgattgac tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt
    22081 agaagcttct gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga
    22141 agtaaaacat agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca
    22201 attcttacaa gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa
    22261 ataggaggaa acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt
    22321 acaaagagac attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat
    22381 ggatggccga tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc
    22441 tgttactaaa ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc
    22501 agcatttgca aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat
    22561 tcatcgggca cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc
    22621 aggaacacca gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat
    22681 gactgtcaat cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac
    22741 ggaactcgct gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg
    22801 aatcgttcca gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg
    22861 atcatatatc tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt
    22921 agactaaatg aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct
    22981 gctgtccaaa tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc
    23041 aggctaccca gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa
    23101 ggaagtattt gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga
    23161 tacacagtta ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat
    23221 tggagctacg ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc
    23281 tcgcttcaaa ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta
    23341 ttatttgcct ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg
    23401 ctgcttgact cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa
    23461 agaagtagaa tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt
    23521 ttctttttca gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata
    23581 aaaaaatttg tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta
    23641 agaggagcaa cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct
    23701 aattaattcg aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct
    23761 tggttattgt tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg
    23821 aaggaaagcc caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa
    23881 caatctctgc cccagtacgg tgggtacgaa atggagtaga ttctgtccat aatctttttt
    23941 caacttatgc cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac
    24001 agcaaaacaa gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc
    24061 agacgctgac tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt
    24121 ggcaggacat gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag
    24181 tcatgtcgca aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa
    24241 tcgaattact gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg
    24301 ctaatggcga agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg
    24361 tgacccaatt aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc
    24421 ttggagggaa ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata
    24481 gttatgggct ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag
    24541 ttgtgacgat tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga
    24601 agtactatct gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa
    24661 tgctagggga gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat
    24721 tattatgttg cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg
    24781 gggctatcta tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt
    24841 taattgtatg gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat
    24901 ttttcagtat gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga
    24961 tttttaaatt agcagtagta aataacacgt attttattac aaggttttta ggacctacac
    25021 tgctgttgaa catgattata tttgtattat tcatttttcc ctttaagaaa ttattcagtg
    25081 atgaataatt tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa
    25141 gaaatgctag acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca
    25201 tttttaaaag ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc
    25261 tatggtacaa tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt
    25321 aagccagtcg gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc
    25381 aatgaccttg actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca
    25441 gatacaacag caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc
    25501 acaaatcgat gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt
    25561 agcaaaacaa gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt
    25621 acaagaacgt attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt
    25681 aagcaacaca agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc
    25741 tattggacgc gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca
    25801 acaaaaacaa gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa
    25861 agaattagct gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca
    25921 agctgatttg aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa
    25981 gaaagctgac ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga
    26041 acaacaacgt ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga
    26101 agcacgcgaa caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag
    26161 ctcagcaaca gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc
    26221 tacacaatct tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac
    26281 agcgcctgaa tcttcagcaa cagaa
//
LOCUS       DACECS010000032        25787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.032, whole genome shotgun sequence.
ACCESSION   DACECS010000032 DACECS010000000
VERSION     DACECS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.032"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            438..992
                     /locus_tag="IWO31_001474"
     CDS             438..992
                     /locus_tag="IWO31_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0301897.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="IWO31_001475"
     CDS             complement(1165..1638)
                     /locus_tag="IWO31_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0301898.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="IWO31_001476"
     CDS             1650..2186
                     /locus_tag="IWO31_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0301899.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="IWO31_001477"
     CDS             2226..2597
                     /locus_tag="IWO31_001477"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0301900.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="IWO31_001478"
     CDS             2881..4152
                     /locus_tag="IWO31_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0301901.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="IWO31_001479"
     CDS             4397..5422
                     /locus_tag="IWO31_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0301902.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="IWO31_001480"
     CDS             5716..6825
                     /locus_tag="IWO31_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0301903.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="IWO31_001481"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="IWO31_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="IWO31_001482"
     CDS             7820..8644
                     /locus_tag="IWO31_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0301904.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="IWO31_001483"
     CDS             8676..9986
                     /locus_tag="IWO31_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0301905.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="IWO31_001484"
     CDS             10134..11393
                     /locus_tag="IWO31_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0301906.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="IWO31_001485"
     CDS             11403..12275
                     /locus_tag="IWO31_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0301907.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="IWO31_001486"
     CDS             12287..13117
                     /locus_tag="IWO31_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0301908.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="IWO31_001487"
     CDS             13157..15340
                     /locus_tag="IWO31_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0301909.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="IWO31_001488"
     CDS             15401..16621
                     /locus_tag="IWO31_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0301910.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="IWO31_001489"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="IWO31_001489"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0301911.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="IWO31_001490"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="IWO31_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="IWO31_001491"
     CDS             19583..19792
                     /locus_tag="IWO31_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0301912.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="IWO31_001492"
     CDS             19785..19934
                     /locus_tag="IWO31_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0301913.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19969..20753
                     /locus_tag="IWO31_001493"
                     /pseudo
     CDS             19969..20753
                     /locus_tag="IWO31_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWO31_001494"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWO31_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0301914.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="IWO31_001495"
     CDS             22456..22689
                     /locus_tag="IWO31_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0301915.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="IWO31_001496"
     CDS             22686..23093
                     /locus_tag="IWO31_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0301916.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24230..24748)
                     /locus_tag="IWO31_001497"
     CDS             complement(24230..24748)
                     /locus_tag="IWO31_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0301917.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8786 a         3930 c         4795 g         8276 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    23341 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    23401 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    23461 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    23521 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    23581 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    23641 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    23701 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    23761 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    23821 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    23881 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    23941 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    24001 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    24061 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    24121 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    24181 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    24241 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    24301 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    24361 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    24421 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    24481 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    24541 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    24601 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    24661 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    24721 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    24781 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    24841 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    24901 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    24961 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    25021 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    25081 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    25141 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    25201 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    25261 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    25321 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    25381 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    25441 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    25501 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    25561 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    25621 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    25681 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    25741 atgctgctca tttcaactta tatgttttag tgaactaccc ccactta
//
LOCUS       DACECS010000033        24505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.033, whole genome shotgun sequence.
ACCESSION   DACECS010000033 DACECS010000000
VERSION     DACECS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.033"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWO31_001498"
     CDS             complement(368..1486)
                     /locus_tag="IWO31_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301918.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IWO31_001499"
     CDS             1957..3264
                     /locus_tag="IWO31_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0301919.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IWO31_001500"
     CDS             3383..4582
                     /locus_tag="IWO31_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0301920.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IWO31_001501"
     CDS             complement(4609..5877)
                     /locus_tag="IWO31_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0301921.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="IWO31_001502"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="IWO31_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="IWO31_001503"
     CDS             complement(7094..7597)
                     /locus_tag="IWO31_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0301922.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="IWO31_001504"
     CDS             complement(7720..8484)
                     /locus_tag="IWO31_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0301923.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="IWO31_001505"
     CDS             8591..8866
                     /locus_tag="IWO31_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0301924.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9582..10535
                     /gene="yidC"
                     /locus_tag="IWO31_001506"
     CDS             9582..10535
                     /gene="yidC"
                     /locus_tag="IWO31_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0301925.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10657..11866
                     /locus_tag="IWO31_001507"
                     /pseudo
     CDS             10657..11866
                     /locus_tag="IWO31_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12176..12397)
                     /locus_tag="IWO31_001508"
     CDS             complement(12176..12397)
                     /locus_tag="IWO31_001508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0301926.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12558..13079)
                     /gene="tnpA"
                     /locus_tag="IWO31_001509"
                     /pseudo
     CDS             complement(12558..13079)
                     /gene="tnpA"
                     /locus_tag="IWO31_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13170..13536)
                     /locus_tag="IWO31_001510"
                     /pseudo
     CDS             complement(13170..13536)
                     /locus_tag="IWO31_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13550..13835)
                     /locus_tag="IWO31_001511"
                     /pseudo
     CDS             complement(13550..13835)
                     /locus_tag="IWO31_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13841..14035)
                     /locus_tag="IWO31_001512"
     CDS             complement(13841..14035)
                     /locus_tag="IWO31_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301927.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14035..14511)
                     /locus_tag="IWO31_001513"
                     /pseudo
     CDS             complement(14035..14511)
                     /locus_tag="IWO31_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14979..15332)
                     /locus_tag="IWO31_001514"
     CDS             complement(14979..15332)
                     /locus_tag="IWO31_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301928.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15460..15999)
                     /locus_tag="IWO31_001515"
     CDS             complement(15460..15999)
                     /locus_tag="IWO31_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301929.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16029..16220
                     /locus_tag="IWO31_001516"
     CDS             16029..16220
                     /locus_tag="IWO31_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301930.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16262..17128)
                     /locus_tag="IWO31_001517"
     CDS             complement(16262..17128)
                     /locus_tag="IWO31_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0301931.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17152..18804)
                     /locus_tag="IWO31_001518"
     CDS             complement(17152..18804)
                     /locus_tag="IWO31_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0301932.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18809..19711)
                     /locus_tag="IWO31_001519"
     CDS             complement(18809..19711)
                     /locus_tag="IWO31_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ0301933.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19795..21108)
                     /locus_tag="IWO31_001520"
     CDS             complement(19795..21108)
                     /locus_tag="IWO31_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0301934.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21176..22120)
                     /locus_tag="IWO31_001521"
     CDS             complement(21176..22120)
                     /locus_tag="IWO31_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ0301935.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22157..23170)
                     /locus_tag="IWO31_001522"
     CDS             complement(22157..23170)
                     /locus_tag="IWO31_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301936.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="IWO31_001523"
     CDS             complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="IWO31_001523"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ0301937.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6968 a         5548 c         4665 g         7324 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9481 gaacgaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9541 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
     9601 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
     9661 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
     9721 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
     9781 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
     9841 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
     9901 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
     9961 tatatcgcga aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10021 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10081 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg
    10141 gcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10201 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10261 cagccggtgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10321 tcacaaacgt tataatgaaa ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa
    10381 acccgccaaa acaagtcgtt actccaagaa aagacgtgac agaatcagca gcaaagccag
    10441 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    10501 gccgcaacgc aggaaggcag cagaagcgga aataaaaata tatgacaaaa aactgtagac
    10561 aaataaaaaa atttctttgt tctacagttt ttttgttaca attaaagaaa acgttcgcat
    10621 ttcgaggagg aatgtataat gatgtccaaa taatcaatgt atagtcgaat ggaagaagac
    10681 aaaatttcgc tagatgcact tgttcctaaa aatcatttag tccgtaaaat tgacaaatta
    10741 ttgtcatttg attttattta tctcattgtt gaatcaactt actcgaccat tggtcgccct
    10801 agcgttgatc ctgtcgtctt agtaaaatta gtattcattc aatatttatt tggaatttgt
    10861 tcgatgcgtc agaccattaa ataagttgaa acaaacctag cttatcgttg gtttcttggg
    10921 cttagtttaa ttgataaagt tcctcatttt tcaacttttg gtaaaaacta tgcacggcgt
    10981 ttcagagaaa ccgatttgtt tgaacagata tttatcatta ttctcgaata agcagttgat
    11041 gctagattta ttcatgaaga aattttgtat atggattcaa ctcatattaa agcgaatgcg
    11101 aacaaacgta tttttacaag agatatggca cataaagaag ccattttata ccaaaaacaa
    11161 ttagaagaag aaatcaatga ggcaagaatt gccgaaggca aacgtccttt tggttcaatc
    11221 attcgtcagg aggtggtcga aagaaaattt agcatcgcag atccagaaag tggttattat
    11281 gtaaaaacag aacgagaaaa acagtttgct tattctgctc ataccgttgg tgatgaaaat
    11341 ggttttgtat tagatgtcat gattacgcct ggaaatctac atgatagccg aatgctcgtg
    11401 ccacaaatcg aacgcgttaa atcacgttat ggtgtagctg ctaatgctgc gtacaaaaca
    11461 ccttggaacg tcaagtattt aatcgaacga aaactacgac ccattctccc ttatatcagt
    11521 ttcgcacaac tactcgagat ggctataaaa aatatgtatc taaatctttt atttgtgaaa
    11581 gttgtccatt attgaaaaat tgtacagaaa gccaaaaaca ccaaaaagaa atacatcgac
    11641 atatttggga agactactta gatgaagcag aacatttacg ccatacggtg tacaatcgtg
    11701 aaaaatataa aaaaagaaaa gaaacgattg aacgcatttt tgcagacgca aaggaaaaac
    11761 atggaatgcg ctacaccaag tatcgaggct tggaaaaaat caggtgcata caatgcttgt
    11821 ttttgtcgca atgaatctga aaaaattagc tacttggcag tgggagcata tgtcgttata
    11881 tcttttatta tacaaaaata gacaaaagta tcagtctaca ctcttagtaa agaggaaact
    11941 gatgcttttg tctacagtct tagagagtgg gacaatagtt ttttcttctt tacgtctttt
    12001 cctctccatg tattttccta attttcttaa tttaacgcca tgagtgccaa gccaatgtca
    12061 atcttggctc cttgtttcct tcataagtgg aaatccgttt ccgatagatc gaaccttctt
    12121 tttcctctaa aagttttttt acattctgct ttgaaataac gccatgtatt attcactaaa
    12181 atttgtcggt ttcgctcact ttttgctttc gtacattgac tacgaacagg tcagcaccga
    12241 ccactttcac attcgtaaat tttgaagaca cgtataaatc cacttttatc ttttcttcga
    12301 ctaaagcgtt gaccgcaaaa gcgacatctg tttcagaaat atattgttcc atatctaatg
    12361 gtaaaatttt ttggttgatg gtataatttt taaacataaa gcactcgctt cctttctgat
    12421 tgggtttggt cactttaatc ctacagggat gagtgctttt tgtgtacctt aaaatcagta
    12481 aatatccatc cgtgaaaacg ggtggctttt taacagccct agaagggctc accactcaca
    12541 gccctatctc tctttaacta tttaaatgga tctacatact cttgtttact tatactgtct
    12601 ctaattcgat catctgcttc ttgttctcaa atatatattt caattgtttt ttgatttaac
    12661 ccaaccgtac tcacgtagta tcctttggtc caaaagcttt tattcccata cttatatttc
    12721 aaatttgcat gtttttcatg gattaaggtt gcacttcgtc cttttaagta gcccataaaa
    12781 ctagaaatac taatctttgg cggtattctt actaacatat gaatgtgatc aggcattgca
    12841 tgagcttcaa tgatttcaac atctttcctc tcacacaatt ttcttaatat ctggccaata
    12901 cttactctta actttccata aatcactttt cgtcgatact tcggggtaaa gactatgtga
    12961 tacttacaat cccatctcgt atgtgctaaa cttttatcgc taaagctatt tcgtccacgc
    13021 gtagaaacgc gcgttttttt gtttcgtacg ctacgccaac gaaactgact aaagtcaata
    13081 aaaaaagtgg ggcaataatc agattttttt ctgactattg tcccactctc tttttattac
    13141 tcttcatctt ctactattcc actaattaat tagttgacag atacaatgct ttcacatcct
    13201 gcacgaacaa actcgggctc tgaaccaatc acttgcgagt tgataaattc aatgaccata
    13261 ggaagattaa tccgacataa agtttaatct gttgtccttc cattatcagt ttagaaatga
    13321 tattagtagg tgtaccgcct aaaagatcgc atagtaccac aaaatcattt aacgaatcta
    13381 tgacagtaag agcttttgtc tgtatgtttc agtgccatct tctggtaata aaggtaacgt
    13441 gtagatattc tcttgaggtc ccataatcat ttttgtactt tttttcaatt cctcacaaaa
    13501 ttttccgtga ctaatcaata tcaattttct actcattttt ggctctccct tatttcgaaa
    13561 tcacacacaa tgcagataaa gccacacaca agtaatacga taaaaatcgc tcgagttggg
    13621 tcatattttt ctttcctaac aaccaataaa cacctcgacg atcccagcga gtaagatttt
    13681 agataatatc atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtac
    13741 cataaaaatt taatatcaat cattgttgca actaatgctc ctaccatgaa cactcttaat
    13801 aaagtcgcta cgtccgttaa tacagttaag cgagattaca ctagatgatc tgttcctgcc
    13861 aaggcaagag ccagaagcgg ttgcctgccc atttgccttt ggtgtgtcgg agctgtccga
    13921 taaagttcac cgcccgatcc gctctggctt tgtcgtagtg acattccgaa agcatgaatc
    13981 ggcttggtgt gtattccttt aatttgggat agccctttgg tctggtttct gccatcagct
    14041 accacctccc aacagagcct ccatcgcatc ttctgctccg acaccaccgc catcaccggc
    14101 aatgatacgg cttctggacg aaggcgtcag accgaactgc tcggaaaatc ggttcataat
    14161 tttcagatag gtctgggcaa taaacacctg tggtacctgc tgccaatagc cggatagggt
    14221 tttcacgatg ataccgtgct gggtaatgaa ttcctcggct tccttccagc gggcgtaggc
    14281 ttggcactaa ccggcaaagg cagccatatc cacatctgtc aagattccga tggcctccat
    14341 ctgctttgcc agacgcttcc attccttctt tgcctctggt tccagccact tcggacaggt
    14401 cggtgccttc ttttccggcg ttgactcttt tgcattgagt gggcgattgc ccggatttcc
    14461 ctccagcttc ttcatggctg tcggggttga ttttcttcct ctggttgcca cagtcgtcac
    14521 ctcccttcaa tgtatttctg ccagtagaca gagaacagag ccaagtcgaa acctgactct
    14581 cttctctatc tgctaacttg tatttcagtc cactcttctg atgaatcgca cccgcaggat
    14641 tttcaagatg ccgcaggcaa attccaagaa gtttctgacc gcatcataac catcgtagcc
    14701 gtgtagccac atccttccac ctcgccgccg tcctcgtcgc tgatataaat gattctgtat
    14761 tcgctcatct gttgttttct ccagtctttt gtttttttgt agtacatata tcactctaaa
    14821 agtgattatt atcaagtcgt ttgaaagcca taatgtacac aatcattcga ctggtttttg
    14881 gttcataatt gaccgccaaa gtgagcaaaa gaaagggacc tctgtctgtt tcagaagtcc
    14941 ctttcggtat ttggttctta gtggttcagg ctgtattctc aaacctcata ctcatcttcg
    15001 aggtctgcct ctgcaatgat ctggccgatg ctgtcgtaac gctggacctg catctggatg
    15061 gcatccttgt ctgctccctc gtccatcatc ttcagcagct tgtctgattc ctttttgtag
    15121 gcccagaaaa ggatctcctg aaggctggtc tcctgctttt tcttgcgaag ggcgaccttc
    15181 ttctcttcca ccgcctcgtt caacttggtg aactctgccc aagcaaggat accgtctcgc
    15241 ttgattgcga tctccacatc cttcagctcc gggatggtct ttgcaccctt gatctccttc
    15301 aaatatttct ttgccttttc gctcattgcc attagcgttt cctccttgtt tttgggattt
    15361 tcccctttgg ttgtgtgtat attgccatag tctctcgact atatcaagtc aatccacagg
    15421 catatactac acaatcattt ggagctgtat ttgtatacat tatgccgatt tatgagagaa
    15481 gaaaaggctc cagccgttcg actggaacct tgggggggtc ggtttcttag gcggcgtggc
    15541 ggtgtagacc ttgaggtagc tgatgttctt ccagacgtcc gtgcccttca gtctgaccat
    15601 gccgtccagc tctcggtcga gggtgaactt gcggaaaagc ttgatgcggt tgaggctgtt
    15661 tgctagctcc ttgccgttct tggcatccag aactgcgtcc tcgatctccg caatactaat
    15721 ttcctgtcta aaccaaaggt tgctcaaggc ttcgggaatg ccgtttgcgg tctgcagggg
    15781 gttgaaagtt tccaatacag caggggtcga aaccacctgt ccgagctgga attttgcgtt
    15841 ccctaaaatc atctgcataa tttcttccat gtgaatcatc catttctgcc gttgtggtat
    15901 tccctttggc agtacagtac tatatatcac tctgaatcgt acatttatca agaagcggat
    15961 ttttgtggaa tcaccaattc gatataatag taattgcatg aaaaatctaa gcactggatt
    16021 ggtaggtgat gattatacgt gacgatcaca tttacacctg tgattcctgc cactacagct
    16081 tccctgccga cgaacagcca gagcgttgtc ctgactgcga aaagacagca actcggctgg
    16141 ataccgaaat cgaaactgaa gattactatc gtgttcgtgc tgaaatcaaa gctgagatta
    16201 aagctttgaa tgcgggttaa cgacaggtac tgaccggcgg ctcaacaccg ccggcttttt
    16261 attaccacat cttgcggaat tcgctgggac gaacaccttc caccttgaaa aaagttctgg
    16321 aaaaatagtt tgcatctgtt atgccaattt ttgttgcaat ctcttccata cttagatttg
    16381 taaagcgtag caagtgcttg gcctgtgtga ttcgtttctg attcagataa ccgacaacag
    16441 ttgtaccgta agtttcacga aaaatcttag ctaagtactg tttattgata aaaaactctg
    16501 cactcaactg atccagcgta atcttctccg tgtaatggtc atccagatat tgttttatgt
    16561 tctgcaattc catttttttt gtagagaaat tcggcacttt gtcaggattc caagaatctt
    16621 ccatcaaatg catcagcagt tcactgagta gaacattgat tttcatatcc cggacatact
    16681 cctgtccctc tgccaccata tgaaggctcc tcagcaagtc agcatactta tctgtttttg
    16741 atgttttaaa gaccggttga ccacctcgtt caaggtattt tttgtagact tccgtcatac
    16801 caatgccatt aaaatggcac caccgcaaag tccacaaatc atcttctgat gactcatgga
    16861 catacggctt caaacagtca ataaacacca tatctcctac ttgaagcgta tatttctgcc
    16921 ccttatactc caatgtgcct gaaccaactt ctaccaggaa acaaaggtaa gatgacaatc
    16981 ctgtacgcct agatgtatgt gccttcagag cagtcagctg tccaatttcc tgcaaataca
    17041 aaagagagga tcttgcaaag aacgatgccg tgtacagcac acgatttgac ttcaccgatt
    17101 tcaaagcgtt agaaaacagt tcctccatta caagatttct cctttctttt cttactcgaa
    17161 catttccgcc agttcagcag ccataatagc ttccatagct tcggcggctt ccttgttctc
    17221 tgcgctgacg ctgatatagg tcttcagctt cggctcagta ccagaaggac gaaccacaac
    17281 agagcagtta tcttccagca taaacttcaa aacatcagac ttcggcagac cattcaggcc
    17341 cttactgtag tctaaagttt caataacctt cttaccacca attatatcga gacccttgtg
    17401 gaattcaacc ataatttcct gcatcttggt aaaacctgcc gcaccgtcaa actcgaagga
    17461 atgcagggtg ttcaaacaat agccatactt tgtataaatc tcgttcagct tatccagcag
    17521 gctgataccc tgagtcttgt agtaagagaa catttcacag attagataag cagcgttaac
    17581 accgtccttg tcacggacat agctaccgga cagatagccg taagattcct caaagccgaa
    17641 gatatagctg tcaaccttac cagccttttc caacatacca atctgctcac cgataaactt
    17701 aaagccggtc agcacatcga tggtacgaac accatagttg gatgcgatac gttcacccaa
    17761 atccatggtg acgatggtct tgaccatcac agggtcagca ggcatttttc catgcttaat
    17821 tctctgagaa cagatgtagt ccagaagtag cagaccagtc tcattaccag acaacagaac
    17881 atagtcacca tgtgcgctct taactgcgat accaacacgg tcgcagtcag ggtcagtggc
    17941 caacagcaga tccgcattgt acttcttggc atactccata cccagagaca tagcttcctt
    18001 aatttcaggg ttcggataag gacaagttgg gaaattaccg tccggcagac gctgttcttc
    18061 cacaacggtc acattgctgt atcccatctc tgccagggtg cgggtaacag gcaccagacc
    18121 ggtaccattc agaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag
    18181 gacggactgc cccttcactt cttcgatgaa agcagtcaga acttccgact gaatggatgc
    18241 aatcttagaa gcatcgccat ccatctttac atcagcaaag atgtccagct tttcgatttc
    18301 tgccagaatt gcagtagcag cctcggtagt aatctgacag ccgtctgcac cgtatacctt
    18361 gtagccgtta tatttagaag ggttatgaga tgccgtaatc ataacaccgg catctgcctt
    18421 cagataacga gtcgcaaaag atactgtagg aacaggaagc agttctggcc acaagtgcac
    18481 ctgaatacca tttgcagcaa agatgtctgc ggcagtcttt gcaaacacat cagatttgat
    18541 acgggaatca tagccaacca ccacggaagc ggcttcatag ttcttattca gatagtctgc
    18601 cagaccttgg cttgccttac ctacggtgtg tacgttcatg cgattagtgc ctgcaccgat
    18661 ggtaccacgc agaccaccag taccgaaaga caaattttta tagaaagagt cctcaatgtc
    18721 cttctcagaa taggaacgca gttcttctat aatatctgca ttggcatgat tcagccaacg
    18781 ctcatattct actttataat tcatattatt atcctctcac atccagaaac tgaatcgacc
    18841 cgtgaatgct gatttcagta gaatcggccg gcacaaaaag gcagtccccc ttatagatgt
    18901 ccaacgattc attatcaaat cgaacggtac cacagccatt gacacacagc aacacacgga
    18961 agctgattga atccgcacga tagtgtaccg tctgtcttct ctcagtgttg acaatcatac
    19021 gagagacctc gaaatgcttg catctgcaaa gcaattccgc agctacacca gcacgatatt
    19081 ttagaacacg aagcggctgt ttcggttctg acaacccttt caagttcgct acttccagcg
    19141 ctttgtctac atgaagttct ctctttttac catttttgtc aacacggtca taatcgtaga
    19201 gacgataagt cagattggaa ttttcctgaa tttctgcaat tagtgcaccc gcaccaatcg
    19261 catgaatcgt tcccgcctca ataaagaaca catcattctt tttgaccgga accttctgaa
    19321 gataacgctc aacagttccc tgagcaatcg cttcacggat cacagcttct tctgttattt
    19381 tctgcagccc atagattaag ctggcatcca gtacatacca catctctgtc tttccaagct
    19441 gcccgttttc tttttcaatt gcatattcat ctgtgggatg tacctgaaca gacaaatcct
    19501 ttttcgcatc aatgaatttg atcaggatcg gcaattctcc tttggtctgc ggatgcgttc
    19561 ccagaaactc tgggtgttct tgcaacacct ccgtcaaggt catcccctga tactctgacc
    19621 agacacaaca aagctcggtc catccgggtg cgtagagcat tccaagtttc cgcaagagga
    19681 gacatctcaa ttccttttgc aaattcatca ttcaggcggt tcccccccac agataatcct
    19741 ttgcggaagg cttcaataaa aacggtttct gtataacgag ccctcctccg ttttttaata
    19801 ctctaactca aatttctgtt tatctttaac attgatatcc ttgccaagct gtacttcaat
    19861 cagcttcagc tcagtatctg ccataatggt atgccgacag ccagcactca tggtgatcac
    19921 atcgccagcc ttaacaacct gctcatatcc atccacgatg gtcttaccag taccggccag
    19981 aaccacccac acctcgtcac ggttcttgtg gctgtgataa ttcatggagt gactagcatt
    20041 cagagtaacc ttaatggtca tgctctctgc ttctacatcc ataaccttat aactacccca
    20101 agacttctct gcgaacatga tctgctgctc gaacttgtca acgaaaggct taatatagct
    20161 ggactgttcc ttatcggaaa ccagaatacc ttcccgagat gcacagatga ccacatcatg
    20221 cagacccata gccaacacag gaacatccag ttcgttcagg atgtgtacgc catcgcaatt
    20281 ctcattcata acacctttac cgataatgga ttcctccata ccttcggtca gagtattcca
    20341 agtccccaga tccttccaca taccagcaaa acgctgtacc tgaatcttgt cctcatgctc
    20401 aacaacggca tagtcgaagg aaatcttagt cagagtatcg tatttgttga acagatcctg
    20461 ataatctaca aagtcaatca gctgatgtgc acgctccatg acatacttca gcttgtagac
    20521 aaacacacca ccgttccaca gtgcaccctg tgcgatatat tcctttgcca cagcctcaga
    20581 aggtttttcc ttgaaggtat ttacgaaagc agtctgctct gcgctctcag gaatgatgta
    20641 gccgtacttc tcggaaggat aagtcggttc aatacccatg agcaccagat tagcttcgcc
    20701 tttgtcagcc tgatcggaca gagatttcag ggcctcaaaa tagtcatctt ctacgtaagg
    20761 gtcaaccggg caaacaacta cggactcttc tggatcaatg cccatcacat ctaccagata
    20821 agcggtagcc aatgcgattg ccgggaaggt atcgcggcga caaggctcta cagatacacc
    20881 cacatcctca ccaagttggt tatggatagc agaaacctgt gtcttagagg ttgcaatcgt
    20941 aacagttgcg tcgttatcta ccttcttaat ctggcggtag acacgctgaa ccatggattc
    21001 atatccatct tcagttttga aaatcttaat gaactgcttg gaacggatgt cattagacag
    21061 cggccaaagg cgcttaccag aaccgccaga cagtaaaaca atattcataa tgatctctcc
    21121 tttttataag aaagaggctg ctgcaatcct caaaaatcac agcaacctcg gtaaattagc
    21181 gttcagcacg catcgggtta ttcaggaagt cctcgtagga cagacggatg ccatcttcca
    21241 actcagtctt atatgtccat cccagcttgg ttgccttgga aacatccagc agcttacgag
    21301 gtgtaccgtt cggcttgctg gtatcccaca gaatctcgcc ttcgtaacca ataacctttg
    21361 ctaccatctc ggtcagttcc ttgatagaca gttccttgcc agtacctgca ttaacagtct
    21421 cgtcaccgct atagttattc atcaggaaca cacacaggtt tgccagatcg tcaacatata
    21481 ggaattcgcg cagaggagaa ccatcacccc agcaggtaac agtaggcaga cctgcttcct
    21541 tagcctcatg gaagcgacga atcaaagcgg ggagcacatg actgtgagta ggatgatagt
    21601 tgtcgttagg tccatacagg ttagtaggca taacagagat atagtcagta ccgtactgct
    21661 tgtttagaaa ctcacagtac ttcaggccag aaatctttgc cagtgcataa gcctcgttgg
    21721 tcttttccag ctcacttgtc agcagacagc tttccggcat cggctgcggt gccatacgcg
    21781 gatagataca ggaagaaccg aggaactgta acttcttaca gccattctcc catgcggagt
    21841 tgatgacgtt catttccaga atcatgtttt catacatgaa gtctgccaga gcgttctggt
    21901 ttgcgataat accgccaacc tttgcagcag cgagaaatac ataatccggc ttttcctgtg
    21961 cgaaaaatgc ttcaacagcg tcctgacggc acaggtcaag ctccttatgg gtgcgggtga
    22021 taatattgtt gtagtcctga cggttcagct cgcgcacgat tgcggaacca gccatgccac
    22081 gatgaccggc tacgtagatt tttgcgtctt tttccatcat aataatgctt gctcctttct
    22141 aaatttagag catctctcat tgcatgagag aaatcaattt gtctgcgatt tcgctcgcag
    22201 catcataaaa tgtatcaaaa tttgaggtgt ctagctgttt gaggtcctca atcacccgct
    22261 ctacgcggtg gaggttttcc ggtgtgacgg aaattgccag acccagtttt tgcaatttac
    22321 aaatagttgt tctatcttcc aaaactgtat cgcgggcgaa cagtgccatc ggacgctgtg
    22381 cgagaataca ctccgcaaca gttccccatc cagcctttgt aataacgtaa tctgccgccg
    22441 ctatatagtc ctgtgtattg ggggtgctgt tagaaataac ggttacgttt tctcccacaa
    22501 gcggaacacc tagcgttgta atgaaatcat acggcgcgtg actgacatca atcggctgtt
    22561 tgaaccgcgc ggacataccg agtgcgacaa atacaggcgg tttttcatgc tgtgatttaa
    22621 tgcgttgcac ttcgtcgtta tgaatcggac gcgccacaac ggaagtcggt gttttttcgc
    22681 tgttttggag aaattccaac atttcttcgt tatttagcgc ataaagcatg gtgtgctgaa
    22741 ttttaccgta acgcttggca tattctttcc aaatatgttc gggtaaaaat tcgcgataca
    22801 gctctgtcca agtgaagttt cctacataca ggaaaggtac tcctgccata tcgcaggcat
    22861 ccattgccca gattggcata tcgcaaagca ctgcgctgac agcttctcgc ttgagccatt
    22921 cggcttctgt ctttgccaaa gaaggaacct ttgccaaaaa atattcacag atctcggtca
    22981 gcttacccgt gtccaccagt agggtgccct cacggttaat cagaccgata tctgtgttta
    23041 ttggacgata gcgcacgcgg tctatcagct ctttgggcag ttcctgctcc aaattgcgct
    23101 ttgcaaaatc cagatgattt gcgccgcaaa ccagtgtaat cttcgcttcg catcggcaga
    23161 gtgttgccat aataatgggc agattccgcg cgatatgtcc gaagccgtga ctggaaagat
    23221 agaaacagat atgtttattc aatgtcagct tacttgactg cgttcttcat tgcagcttcg
    23281 cgctttgcca attcacggtc atgctttgcc atgatgttaa ccagttcctc gtaagaggtc
    23341 ttctgcggat tccaaccgag aacggtcttt gccttagtcg ggtcgcccca caggttgtca
    23401 acgtcagtcg gacggaacca ctgtggattt acacaaacga gcatcttgcc ggtttcaaca
    23461 tcgtagccct tttcatcaat gccagtgcct tcccaacgaa tggtcatgcc atttgctgcg
    23521 aatgccttct cggtgaagtc acgaacagtg tgctgctcgc cggtagcgat cacgaagtcc
    23581 tcgggagtct catgctggag aatcatccac atgcactcaa catagtcctt tgcatagccc
    23641 cagtcacgca gggagtccat gttgcccagt tccagatggt cctgcaggcc ttctgcaatg
    23701 cggccggcag cgagggtgat cttacgggtg acgaaattct caccgcgacg ttcggattca
    23761 tggttgaaca gaataccgtt gacagcgaac atgccataag cttcacgata ttccttagtg
    23821 atccagaagc catactgctt tgcaacagca taagggctgt aaggatgaaa cggagtggtt
    23881 tcacgctgag gaacttcttc aaccttgcca tacagctcag aagtagatgc ctgataaatc
    23941 ttagtcttct tcgtcaggcc cagaatacgg caagcttcca gaatacgcat aacgcccaaa
    24001 gcatcaacat cgccggagta ctcaggaaca tcaaaagaaa cctgaacatg gctctgtgca
    24061 gcgaggttat agatttcatc aggctgaaca atgctgatga tacgaatcag agaagatgag
    24121 tcggacaggt caccgtcatg aagggtgatc ttgcccatca aatggtcaat gcgagcggta
    24181 ttggaaatag aagcacggcg agtgataccg tgaacttcgt agcccttctc tagcaggaaa
    24241 tctgccagat aggaaccgtc ctgaccagta ataccagtaa tcagcgcttt tttcatgata
    24301 cattatcctt tcctatgtaa gtgataaggc catataaatg gctcgtaatt tctaatactt
    24361 ttcaagaata cacgataatt ttcgatattt catagaacaa tctacgcacg cattagcaca
    24421 atattagttt ttcgtgaatt ggaatggcaa cagttttttt gacaaatttt ataaggtgca
    24481 gaacttcttt ccgtatgcta ttccg
//
LOCUS       DACECS010000034        24390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.034, whole genome shotgun sequence.
ACCESSION   DACECS010000034 DACECS010000000
VERSION     DACECS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.034"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IWO31_001524"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IWO31_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0301938.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /locus_tag="IWO31_001525"
     CDS             complement(1552..2973)
                     /locus_tag="IWO31_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0301939.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IWO31_001526"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IWO31_001526"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0301940.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="IWO31_001527"
     CDS             complement(5384..6640)
                     /locus_tag="IWO31_001527"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0301941.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7220..8521
                     /locus_tag="IWO31_001528"
     CDS             7220..8521
                     /locus_tag="IWO31_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0301942.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="IWO31_001529"
     CDS             complement(8590..9189)
                     /locus_tag="IWO31_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0301943.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9443..10324
                     /locus_tag="IWO31_001530"
     CDS             9443..10324
                     /locus_tag="IWO31_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0301944.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10337..11947
                     /locus_tag="IWO31_001531"
     CDS             10337..11947
                     /locus_tag="IWO31_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0301945.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12024..12683)
                     /locus_tag="IWO31_001532"
     CDS             complement(12024..12683)
                     /locus_tag="IWO31_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0301946.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12966..13631)
                     /locus_tag="IWO31_001533"
     CDS             complement(12966..13631)
                     /locus_tag="IWO31_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301947.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IWO31_001534"
     CDS             complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IWO31_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0301948.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IWO31_001535"
     CDS             complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IWO31_001535"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0301949.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IWO31_001536"
     CDS             complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IWO31_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0301950.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15945..16265
                     /locus_tag="IWO31_001537"
     CDS             15945..16265
                     /locus_tag="IWO31_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0301951.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16377..17246)
                     /locus_tag="IWO31_001538"
     CDS             complement(16377..17246)
                     /locus_tag="IWO31_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0301952.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17342..18616)
                     /locus_tag="IWO31_001539"
     CDS             complement(17342..18616)
                     /locus_tag="IWO31_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0301953.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IWO31_001540"
     CDS             complement(18642..19151)
                     /locus_tag="IWO31_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301954.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="IWO31_001541"
     CDS             complement(19326..21461)
                     /locus_tag="IWO31_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0301955.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="IWO31_001542"
     CDS             complement(21544..23199)
                     /locus_tag="IWO31_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0301956.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="IWO31_001543"
     CDS             complement(23201..23515)
                     /locus_tag="IWO31_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0301957.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="IWO31_001544"
     CDS             23685..23945
                     /locus_tag="IWO31_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0301958.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="IWO31_001545"
     CDS             23959..24273
                     /locus_tag="IWO31_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0301959.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6949 a         5044 c         4397 g         8000 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttaaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
     9301 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
     9361 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
     9421 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
     9481 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
     9541 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
     9601 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
     9661 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
     9721 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
     9781 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
     9841 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
     9901 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
     9961 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    10021 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag aagaaggaac
    10081 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    10141 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    10201 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    10261 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    10321 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    10381 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    10441 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    10501 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    10561 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    10621 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    10681 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    10741 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
    10801 ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    10861 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    10921 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    10981 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    11041 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    11101 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    11161 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    11221 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    11281 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    11341 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    11401 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    11461 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    11521 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    11581 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    11641 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    11701 cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact cctttgcact
    11761 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    11821 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    11881 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    11941 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    12001 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    12061 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    12121 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    12181 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    12241 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    12301 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    12361 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    12421 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    12481 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    12541 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    12601 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    12661 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    12721 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    12781 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    12841 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    12901 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    12961 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    13021 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    13081 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    13141 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    13201 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    13261 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    13321 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    13381 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    13441 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    13501 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    13561 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    13621 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    13681 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    13741 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    13801 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    13861 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    13921 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    13981 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    14041 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    14101 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    14161 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    14221 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    14281 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    14341 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    14401 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    14461 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    14521 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    14581 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    14641 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    14701 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    14761 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    14821 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    14881 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    14941 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    15001 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    15061 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatccg
    15121 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    15181 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    15241 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    15301 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    15361 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    15421 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    15481 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    15541 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    15601 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    15661 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    15721 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    15781 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    15841 attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat gtgtagtgct
    15901 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    15961 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    16021 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    16081 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    16141 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    16201 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    16261 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    16321 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    16381 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    16441 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    16501 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    16561 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    16621 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    16681 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    16741 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    16801 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    16861 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    16921 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    16981 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    17041 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    17101 gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    17161 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    17221 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    17281 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    17341 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    17401 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    17461 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    17521 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    17581 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    17641 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    17701 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    17761 tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat agcatcttca
    17821 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    17881 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    17941 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    18001 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    18061 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    18121 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    18181 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    18241 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    18301 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    18361 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatcttcga
    18421 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    18481 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    18541 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aacgctgtcc tgcatcaagt
//
LOCUS       DACECS010000035        24220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.035, whole genome shotgun sequence.
ACCESSION   DACECS010000035 DACECS010000000
VERSION     DACECS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.035"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IWO31_001546"
     CDS             complement(105..629)
                     /locus_tag="IWO31_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301960.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO31_001547"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO31_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0301961.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO31_001548"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO31_001548"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0301962.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IWO31_001549"
     CDS             complement(2351..3031)
                     /locus_tag="IWO31_001549"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0301963.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IWO31_001550"
     CDS             3157..4026
                     /locus_tag="IWO31_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0301964.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IWO31_001551"
     CDS             4052..4567
                     /locus_tag="IWO31_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301965.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IWO31_001552"
     CDS             complement(4614..5177)
                     /locus_tag="IWO31_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0301966.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IWO31_001553"
     CDS             complement(5232..5411)
                     /locus_tag="IWO31_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301967.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IWO31_001554"
     CDS             complement(5488..5997)
                     /locus_tag="IWO31_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301968.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IWO31_001555"
     CDS             complement(6093..6473)
                     /locus_tag="IWO31_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0301969.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6804..7553
                     /locus_tag="IWO31_001556"
     CDS             6804..7553
                     /locus_tag="IWO31_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0301970.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7737..9014)
                     /locus_tag="IWO31_001557"
     CDS             complement(7737..9014)
                     /locus_tag="IWO31_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301971.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9093..9737)
                     /gene="pcp"
                     /locus_tag="IWO31_001558"
     CDS             complement(9093..9737)
                     /gene="pcp"
                     /locus_tag="IWO31_001558"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0301972.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9936..10880)
                     /locus_tag="IWO31_001559"
     CDS             complement(9936..10880)
                     /locus_tag="IWO31_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0301973.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10882..11568)
                     /locus_tag="IWO31_001560"
     CDS             complement(10882..11568)
                     /locus_tag="IWO31_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0301974.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12042..12362
                     /locus_tag="IWO31_001561"
     CDS             12042..12362
                     /locus_tag="IWO31_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0301975.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12516..13421
                     /locus_tag="IWO31_001562"
     CDS             12516..13421
                     /locus_tag="IWO31_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0301976.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13418..14191
                     /locus_tag="IWO31_001563"
     CDS             13418..14191
                     /locus_tag="IWO31_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0301977.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14372..15796)
                     /locus_tag="IWO31_001564"
     CDS             complement(14372..15796)
                     /locus_tag="IWO31_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0301978.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16007..16453
                     /gene="nrdI"
                     /locus_tag="IWO31_001565"
     CDS             16007..16453
                     /gene="nrdI"
                     /locus_tag="IWO31_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0301979.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16518..18500)
                     /locus_tag="IWO31_001566"
     CDS             complement(16518..18500)
                     /locus_tag="IWO31_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0301980.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18514..19410)
                     /gene="murQ"
                     /locus_tag="IWO31_001567"
     CDS             complement(18514..19410)
                     /gene="murQ"
                     /locus_tag="IWO31_001567"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0301981.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19413..20474)
                     /locus_tag="IWO31_001568"
     CDS             complement(19413..20474)
                     /locus_tag="IWO31_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0301982.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20685..21212)
                     /locus_tag="IWO31_001569"
     CDS             complement(20685..21212)
                     /locus_tag="IWO31_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0301983.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21279..22781)
                     /locus_tag="IWO31_001570"
     CDS             complement(21279..22781)
                     /locus_tag="IWO31_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0301984.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23039..24160)
                     /locus_tag="IWO31_001571"
     CDS             complement(23039..24160)
                     /locus_tag="IWO31_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0301985.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
BASE COUNT         6919 a         5167 c         4264 g         7870 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tagtaagaaa aaaggcaaaa aaaacagaaa
     6601 gtaggtcctc caagacactc ttcctgttcc caatataata aatgaaagta agaaattata
     6661 ttttcaagaa aacaaacaga aaattatcat aacacaaatt atttataatt gaaagactag
     6721 ttttttttca agaaaagcat acaatagatt gtgttagtaa atacagaaca cactacattt
     6781 agtgtcttaa ggagtcctca tacatgaagt catattttca gtttttagct catcagctaa
     6841 aaggctggcc gcaacaaaat tattatcttt tctttttcag tcttggctgc caggtcatga
     6901 ctcttgtcaa tagtcccatc acttggctgt ccgttattac atttatcggt accacgcttg
     6961 gtgtattgtg tattttatcc atcaatgcgg caaaatcagt caacggtatt ttaggtattt
     7021 tgtctgcgat ttgttttatt atcgttgggt tttcagcaaa gaactattta agtatcggtg
     7081 aacagattgc ttatatgatc acattggata tccccgtatt gttaagtaaa gaatggaatc
     7141 ataatatggc ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc gcaatcatca
     7201 gtactttcct tgtctatctt gtttcaggat tcctgattgg tcgttataca gatgatcctc
     7261 ggccgtgggt agatgccatt gctttttcta ttagcttgac tgctggtatc atcagtttct
     7321 tgcgttacaa caaccaatat tactggtggt tagcttcagg gcttgctcaa atggtccttt
     7381 ggtatatttc atttacgcat ggatctgcaa gtttggcgat gttcgtgaac agctctgttt
     7441 acttgctgaa tgatgtcctt gcatttaccg tttcaccttg gtataaccaa aaagaacgtg
     7501 aacgtatgca aaaaatggaa gaagaatact acaacgcaca acaacacgca taaaaaaact
     7561 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     7621 gaatcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     7681 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc tattttttat
     7741 agtaattcct tcagatactt tcttgcctca tcattgaaac gcagtgattc ttctaacttc
     7801 tgataatatt ccaacgcttg tttctgagct tcagtaaaac gataatgttt tcctttccat
     7861 ttgtcgattg tctcttgcac atttccagat tggatatctt tcactactag ttgccatttt
     7921 tcttttgaga ctgtacggtc tttcttatca gcagattgta tagcttctcg ttctttacgc
     7981 aatactttta aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa cccttctgta
     8041 aaaccatagc gacaatattg cttccaaata aatagataga tttttggttg gatgaatcgt
     8101 tggtttttcg caaaatattc catctgagca gtgacttctg gtgttcgttg acgttttgtc
     8161 tttctcaaca cggaaataaa tgtttcaaaa taagcattag gatgtactgg cacttcttca
     8221 tatacgatgg attgccaatc tttacgtaaa aatgcgcgat agttgatcaa gaattcttct
     8281 acatccttta ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt
     8341 tttgactgcc cattgatgtg ttcacttatt ttcttgattt cattttccaa tgcttgataa
     8401 tccgctatgt aaatcggcat ttctacttgg cgaaattctg cgagccgctg agctaatttt
     8461 atatccggta aatatggctg tttcatccga cgattttggt acaagacaaa acagtatttt
     8521 tgccatagtc cttcaggaaa atcgatattc ttctgcttaa gccataataa ttcgtctaag
     8581 gccagatcaa cttttgtcat tccctgatgg atcgaagctg ccactgtcgg agagacatgc
     8641 aggtgatcgg caaagtggtt gatgcctagt tttaggatct ttccggtttt tggcgattta
     8701 acgatgtatt gcacttttaa ttctctacca cactcacaat acaatttttg ctggcttcgt
     8761 tttgttcgaa aaaacggatt gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa
     8821 taatgttctt ccataaaacg agagctgatt agatatcggc ggtgcttatc gactagttcc
     8881 cgctgttttt tcgttaattt tgcatagatc tcccaacgtt gactgcttgt gtattgttta
     8941 gaaattttga tgggatagct tttgctgatt tcttttggaa accattcttc aaagacggat
     9001 tgccattctt ccacggctgt ttctttcctt tctaattaca aaaagctggc tactaacata
     9061 acgttagcaa ccagccagtg tttcttgcta aattagtgag ttgttccggc atttccggaa
     9121 atatcttctt tattttctac tgctgcttca actgcataag tcaaagaaga agcaatgtct
     9181 tctaaagaca tagaaggcgt acccactgga cgatcgatca cttgatcggg tgagaatggc
     9241 acgtggatga acccacctcg aattgccggg aattgtgttt cgatcaaatg aagcaaacga
     9301 tacatgatat cattgcagac aaatgttcct gctgtataag agatgtacgc tggtagacca
     9361 tgatcttgta cattttttgt cattgctttg attggcaatg aggtaaagta agcggctgga
     9421 ccgttttctt ctaaagccgt atcgaatggc tgatttcctt cattatcagg gattcttgct
     9481 tctgctagat tgattgcgac acgttcgaac gagactgctg accgcccacc tgcttgtccg
     9541 acacagatca cgacatctgg ttggtgctgt tggatggctt ctttgactac ttgactgctt
     9601 ttttcgaata cggtcggtac ttccactttg atgatttcag caccgctgat ttcatctggc
     9661 atttttttta ctgcttcata ggctggattc actttgtccc caccaaatgg atcaaaacct
     9721 gttactagta ctttcatctg ttgttcctcc gtccgattga tttaacatat ggttatcttt
     9781 tatcatacca ttttccttga acagcgtaaa taaaggaaac aatagtttaa ataacataac
     9841 agatcaaact taatatattc ttataaaaaa atagtgttcc actacaatgt tagaagaggt
     9901 tgcgacaatt attctgtcac aacccctctc ttctattatt taaatagaat catataagca
     9961 atttggaaaa ctagcataaa taacgcaata aacaactggt ttttgatcac tccgtatttg
    10021 tctttcatct ccagcatcgc tacaggcacg atattgaagt tagcagccat cggtgtcagc
    10081 agggtcccac aaaaaccgca agtcaatgca accattccta ttaacgctgg attcgctcca
    10141 tgactaaata cgaatggtgc accgatccca accgtcatca cggtgatggc cgcaaatgca
    10201 ttccccatga ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat gccgatgttc
    10261 acatttcctt ctggaacgat cgtacctact gcattggaaa tcactgtccc aactccagcg
    10321 cttgtaaata ccgcacctag agaagctaaa agcattggca acatgcttaa cggccctact
    10381 gtgcccagca tatctgctgc atcatctaaa aatgtacttg gtcgattatc tcgagaaaag
    10441 aacatcaaga ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa
    10501 ttggtaaata gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg aataaaaatt
    10561 ttcattccta gtttgtctga cattttttcc atatattctt ttgatggaag ccggctttct
    10621 cctttgctga ctttcttagc aattgctggt atggtcatcg caaagatcaa gccaccgttc
    10681 acgattgccg gaagccatct tcctcctgca ataactaccc ctaagaccgt ccagaaaaag
    10741 gctgacccat aacgatgagg attggtttta tctgttaaat ttcttactcc agtgtaaatc
    10801 aatacgagtc ccatcacgat ataaaggatc tccagtaatt tatcgcctaa taaaatatca
    10861 ggatttgtaa aaaagtccat cttattgccc ctttcctgga taatatttct tcgctaattt
    10921 cttttcttta agatagaagt agacacttgc cacaactaaa gccgtgatag ccactggaac
    10981 ttccactaaa gcaagctgtc ccaatgttac gtcatatcct agatctttca gtgttgattg
    11041 gactagcaac gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc agcagacatt
    11101 ttccatagct gaagccatac cttttagttc ttcttcatgc ttaggatgga cttctaaccc
    11161 ttttgattcg atggtcccca atgccatcgg taaaatgatt ggacgaacaa atccagcaac
    11221 tccaccaaaa ctcacgttaa aagccgcaaa gatcattcga aagatcccat aaatatctac
    11281 aatcatacca gcagatactt tcttgaatcg cttgatcaag gaagccgctg attctttcag
    11341 accatttctt tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg
    11401 attgtttaca aaacttgtac ctaaaagttc taacattcct tgaatgccaa gaccgccagt
    11461 caaagctgta acaactaaag caaccattac gatcaatatc gagtctaatt taaatgcaaa
    11521 cccgagaata acaatcaaga cgcctaaaag gctaagataa ttttccatat attttcctcc
    11581 ataaaatgac aatatttttt taattataaa gcaaaacaca taaaaaatat agttgttttc
    11641 tcacgaaata gacactattt ttaaatattt ttcgttattt ttttatcaca aaaattatct
    11701 tacttattgt aaggtaaagc tttttttaat ttattgcccg ataatatata gaaataaggt
    11761 ctagttactg ttttttttaa atgattaata aataacaact tctttctatg aaattgcaaa
    11821 gcattcatct tcttttccgc taacgatata tccgtcagtg cttggtacac tttactaaaa
    11881 agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga tatacaatct
    11941 gccaccgggt aggataaaga ggatgatcat tccattaggt ctttgaaggg atgattttcc
    12001 tctttttgtg tattttttta tagttaagga ggaatgtaag tatggcatgg atggaattaa
    12061 tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt gatggttttt
    12121 ctaaaatcaa ttattctttt tacacagtga ttggcatgat tgctagtttt tactttttgt
    12181 caaaggcaat caaaagtttg ccgatgagtc tctcttatcc tatctggaca gggattggcg
    12241 cagttggttc ggtcataatt ggtgtggtct tgtttcatga taagctgaat ctttccactt
    12301 ggttctttgt ggggatgcta ctcatcagta tcattggcat caagattact agcggccact
    12361 agacacttgg aataagtact gatcagaaaa aataagcctt tttatttaat attcaatttt
    12421 tttatgaaaa aataagaaat aagtccaatg acctagcgac ttctccactc atcaggtgta
    12481 tgcttgtact atctatagaa aaggagtgaa tatttttgaa aagtctttcg gtccaagaat
    12541 tatccaagaa ctttggtcac aaacaagccc tcagcgatgt cagttttagc ttatcccctg
    12601 gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta
    12661 ttttaggact gatccattac tcagatggaa caatctgtat tggtgaaaat gagatttctc
    12721 cctctagtca taaagggttg gaacaagtag gagcattgat cgaatacccg ggtatctatc
    12781 cattcttaac aggctatgat catctgaagt tattctctga aaccaacgat gtatctgcta
    12841 tcgacactat cgtgaatcag ctcaagatgg aaaagtacat tcacaaaaaa gcgaaatcct
    12901 actcgttggg aatgaaacaa aaattgggaa ttgctcttgc cttactgaac catccgtcta
    12961 tcgttatttt agatgagccg atgaacggtt tggatccaca agcaacaaga gatgtgcgaa
    13021 aaatcattac cgacttggct actcagggca cctctttctt gatctccagt cacttactca
    13081 gcgagcttga aaaaatggtc gatgagctga tcctgatcaa tcaaggaaag atcatcaaac
    13141 aatgtacaat ggctgaattg aaccaaacag cgcaagattt catcgttatc tctacaacaa
    13201 atgatctagc tgctcgaacg atcttgacag aagctggata tactttggaa gatcctgata
    13261 aaatcaagct tttaaaagaa aacgaagaat tattaggaac cgtgatccag ctattgtctg
    13321 cacaaaatat ccatgtgaca gacgtccagc atctgacgaa tgatttggaa tcttctctat
    13381 tggaaattct ggattcagta aagggggaag aagaaaaatg acattgcttg taaaacaaga
    13441 attatttaaa ttgatcaaga aaaagtcaac tgctgtttta tctgttctat tagttgtttt
    13501 gctcatagga acagcacttc tagccaaaaa atacacgacg atcattgatc cagttgaaat
    13561 gactgctcag ctttttagtg ccacatcttg gattgtcttt atcatgattg cagctgcaag
    13621 cacgatcatt tcgatggaag cacaatatgg tacattgaaa aatctccttt atcgaaagta
    13681 ttctcgagga gaaatattag taagcaaatg gatcacatta gttatttatt ctgtttattt
    13741 atacctttta gctattattg tcactgtgtt gatgaaactg attcttttcc cttcgatttc
    13801 cttcacggag aaggtttcta ctggtcaaac actgatccaa agcttgttca cttatacttt
    13861 gggtagttat atcggtttat ggctgatttt aagtttagtg ttgatgattg cttgctttat
    13921 caatagttcc ggcgcatcca tctcagcagg aatcgtcttt tattttgctt cgtcaatcat
    13981 atctggtatc cttattgcac tcatccaaaa atgggagtgg atcaaatgga atccgatcag
    14041 tatgttgaat ctgcaaaacc aaattggaaa cgaagagatc atgaagcaat tgactcatct
    14101 ttctactaat caaatgctat tcggtaattt agcttacatc gttttgttct tagcattagg
    14161 atatctcgta tttaagcgaa aaaatgtatg atgactcatc aaaaaagagg ctgtgaaaga
    14221 gacgttcagc cttattgccg attggctgtc tcttaaacac cgtttattct gaaaaaggag
    14281 ctgtgacaac atttgtgtca cagctccttt cctagtatgt ttttgaataa acgacaggct
    14341 cacaacctct ctttatactt cttttatact tctatacgtg aagcatccca gtggcaaata
    14401 aaaccattgc aatcactccg accatcgtca cacctataag taaaatctta tctaagtgga
    14461 tatgttgtac aggctcgttt tgctccttac gtgctttgag gtaaaaataa atgcctggca
    14521 agaaaagaac aagaacaagc attacttcat taaaggctac gactagcata gcccatactt
    14581 gaaaggcact tgctaaaagc cctactaaaa attggaccca ttctttccgc tgataagaaa
    14641 gtttcatttg gtacaatcct aagaaaagcc acgtgaagaa aatagctgct gtaccaagtg
    14701 tgtaagcaaa gttataagca ctgtcagtga ataataatga aagtaaaaag acattcgtac
    14761 ataacgtcgt caatacgata gcataagttg gtgccccatt tttgttgagt tttccccatg
    14821 ctttaggcaa aagtccgtct tcagccatgt ttcccattgt ttcaatcggt aagatcgtcc
    14881 atgagagcca aacaccaact aaagaaatga tcaaaccgcc gttgatcacg actgctcccc
    14941 atttaccaac catcatttca aacacatagc ccatagcagg ctgcgccttg atcgtttgca
    15001 gctgattcat cgtcattgtc ccatatggaa gcatggaaac caagacgtaa ataataatca
    15061 agccgaatat ccctagaatc gttgctttcc caacatctga ttgttttttt gctcgtgaac
    15121 ctacgacaga ggctccttca ataccaacaa atacccaaag catcaccatg atgcaactca
    15181 atacttgact ggaaaacggg atcggctgtc cattcgcatt caatataatg tcgctagaaa
    15241 agttatcaat gaattgttgg atcgaaaacg tgctgatagc tactacaata aataaaaata
    15301 atggcactaa tttacagaat gtgacgatag cattgacgaa actagcattt tcgactcccc
    15361 gattcactag ccatgcatat ccccataaaa agactgtact catcacgatc gatgccagat
    15421 tttgcccacc ttcaaatact gggaagaaat atcctaatgc gctcatcaac aatgttgcaa
    15481 aagctacgtt gcctaaccag catgtgagcc agtaacccca accgctgata aaaccagcga
    15541 aacggccaaa accttcttct gcgtaactaa agatcccgcc ttttaaatct ggacgttttt
    15601 tattgatatt attgatcgac aaggagagca tcaacattgc tgttcctacg agtacccaac
    15661 tgaaaatagc tgggccgcca gccgatccgg agatgtccgt cataatacca aatacgccac
    15721 ctccaatcgc tgaaccaatt actaaggcag caagtggaat taaaccaatt ccttttttct
    15781 gttgctctgt atccatgtaa gcatcctcca aaataaaacc gcttttatca aaaagtataa
    15841 caaacctttt ttatttttac ttaattaata aaaactttaa tagaaatata attctaccgg
    15901 cttttttctc attatacaag caaagcgcct agtccctttc caaattacac caccaaaaaa
    15961 cttaaaaaag atgctatact aaaaggaaag aattggagga tgatccatga acattttata
    16021 tatatcgatt tcaggaaata cgcgtgcctt tgccaaacat ctagcagaat atgctgaaaa
    16081 aatgcatgca gaagatcctg tcaatcctga agttacttta aaagaaatcc atgaaaatag
    16141 tgattttacg aaagaaacag aaccattttt tactttcgtt cctacttatt tagacggcgg
    16201 aaatggttta gataacggtg atacagaaat cttgaccgaa acgatgcgtg aatatctgga
    16261 acatgaagac aatcataaac tttgtcttgg tgtagtcggt agcggaaata aaaacttcaa
    16321 taatcagtat tgtctaacag ccaaacaata tgcacaaaca tttggctttc ctttcttggc
    16381 agattatgaa ttacggggaa caccatctga tgtgacacga atttatcaga ttttagcaga
    16441 aaacaataaa taataaaaaa gaagaagctg aaaaatagat ttcagtttct tctttcgttt
    16501 ctattgtttc tatcatatta agctttgaca tccatcacag gtgcttttgc ttttgcttta
    16561 cctgtttttt ccaagacaaa gttttttaca cgatccatat tggttaccaa gacgatcatg
    16621 gatgtttctt tgcctgctgc tttgatcgct tctaagtcag cttctgctac aagcgtatca
    16681 ggtgtcactt gctgcccttc tgttactttc acatcaaatg gcgtaccgcc taagtcaact
    16741 gtgttgattc ccatatgaag caagatttct aaaccatttg gcgttgtgat cccaatagcg
    16801 tgtttcgttg ggaagacaga gctgactacc ccagtaactg gagaatagat atgtccgttt
    16861 tctggttcta caaagtagcc ttcacccatc atcttttgtg caaaaactgg atcacttgct
    16921 ttatctaaag aaacaactgt tccgtcagca actgatgaga ggatagtcac actttcagct
    16981 gaagtttgag gtactacttt ttcaggcact tcatttactg tggctgtttc agtcgctaat
    17041 tcttcttttt ctaaacggtc tttaggaata ccgaagaaga atgtagcaac gaagccacca
    17101 gcataagctg ccaataatcc aaggatgtag cctaaccatt taccgtcaga gatcaatggg
    17161 atcaaagcgg ctcctgaagg tccaatagcg atagccccga catttccgat cattccgatc
    17221 actgccccgc cgatacctcc accgatacaa gcagtaataa atgggcgtcc tagaggtaat
    17281 gttactccgt aaatcaatgg ttcgccgatt cctaggatcc cgactggtaa agctcctttg
    17341 atcatttctg ttaattcttt atcttttttg cagcgaatcc ataatgccaa tgctgcaccc
    17401 acttgtcctg caccagccat cgcaagaatt ggaagcaata gcgtacgacc agtttcatca
    17461 atcatttgca catggattgg agtcaaaatt tgatgcagtc cgaacataac catcggcaag
    17521 aataatgtgc ctagaacgaa gccggcaaat gctccaccaa cgccaagtac ccagttgatc
    17581 ccaccgacca tgccatcaga aatgaaacct gctaaaggca tgatgaagaa aatttcaatc
    17641 agaccgatgg caatcaatga aagagttggt gtaacgatga tatctaccgc atcaggtact
    17701 actttatgca gttttttctc aacgattgac aatagccaaa cagcgaagat gacacctagg
    17761 atcccacctt gtccagctgc aagagcttca ccagtaaata agtttttcag tggtgcttcc
    17821 ggattcatcc ctgtcagcat cacaacggcc ccgataactc caccaagagt tggtgttgca
    17881 ccaaaaactt gtgcagcatt gatacctgta tagataacta ggtagctaaa catcccgttt
    17941 ttcaagatgt tcatcacatc gatatattgt tgccatgttg ctgcatcaag tgttccggct
    18001 gtgactaagt ttgctaaaac tgcggcaatc cctgccacga tcccagtacc gacaaatgca
    18061 ggaatcattg gcacaaagat attagaaatg tcttttaata ccgatttgat tttagatggt
    18121 ttttgtttcg atttttgtgc cgctttcatt tcttgtgccc gttcgtttac acggtctttt
    18181 ccagatgctg atgaatttgc tgtagctgta cttacagaag aaagtttttc tcctaatttc
    18241 acgcctgcca aatcaaccat ttgctgtgct actttgttca ctttcccagg tccgatgatg
    18301 acttgaagct gttcatcttc tactacacca agcactcctg gaatcgcttt taattcagag
    18361 acatttacaa gatcatcatt gatgatcttc atacgcacac gagtcatgca gtggacaatg
    18421 ctttctacat tgcccatgcc gcctgcccct ttataaattt gttttgccag ttcttccaaa
    18481 gtcagttcgt tttttgccat ggtcttttcc ccctcataag gtccctttta caaatccgtt
    18541 tgcttcagct aattttgctt gagcttcctc tttatcacta tttgtcaaaa tcatgacgat
    18601 agccagctta acattttgat ctgcttcttc aaagtacttt tcagctgttt cgtaatcagc
    18661 ttctgtcgct tgcatgatga ttcgttttga gcgttcgatt agtttttcat ttgttggttt
    18721 gacatcgacc atcaagttat tgaatacttt acctatacca atcatactga tagtagaaat
    18781 catattcaag ataagttttt gtgctgttcc tgacttcaat cgagtagagc ctgtcaaaaa
    18841 ttctggacct gcatcgactt cgattgggaa atccgcgtgt ttactgattt cggcttgttt
    18901 attgcaagaa atcgttcctg tgacagcacc gatcgatttt gcgtaatcta atccaccgat
    18961 cacatatggt gtacgaccgc tagctgcgat accaataact atatcatttt ttgtcaaatt
    19021 cagcttgatt agatcttctt gccctagctc ataagaatct tcggcccctt cgacagctac
    19081 agtcattgct tcttctccac cagcaattag acctatgacc atttccggtt ctacaccgaa
    19141 tgtcggtacg cattcagcag catctaacac acctagtctt ccacttgttc cggctcccat
    19201 ataaatcaga cgtccacctt ttttaaagga ctcaatcgtg ttagcaatca ctggttcgat
    19261 acttgtcagt tctttttcaa ccgcagcggc cactttctga tcttctttgt tcattagttg
    19321 aacggcttga gaaatactca tctcatctaa tccaaaagtt tcttggtttc tgcgttccgt
    19381 tgttaggttc tctaaattga ttttgctcat ttttatcttc cttcttttct tataaattga
    19441 tagttcatac cagctttgat ttggttgatc aatggtagct cttctttgat aactttagct
    19501 actacattca ctttttcatc cgctggcaaa tcatatttgg ttaattggat ctcacccata
    19561 tagcgcaaat acttttcgtt atctaaagta atagaccctt tcatacgcgt agctgtattt
    19621 cttgctggga taggcggaat ctctcgaaaa cgtgcatcta cgcttcgtat gacatctctt
    19681 gcatcgtctt gccgattcgt atgcttccct aatatatatt cgtaaaattc ttcatctaca
    19741 ggttccgtat ggagcaagac tgtctgttct ttttggaaag aagcaaattg ctcctgaacg
    19801 ttttctgaca gacctgcatc tccgatgtaa acaatatccg tgttacttgc taatagatct
    19861 agagcgcctg caagaggatg gatccctcga tgttcttcca aagttggcaa tccttgatat
    19921 agtgggcctc tcaactctgt atctcctggt acaaaacctt ggacaaggag accttgaatt
    19981 ttgagccatt gatttttttt ctgataccat tctttgtcca gccctgtttc gggacgtgga
    20041 taataattat gccaagcttc aatctgtgag aaatcagctt ctgcttcttt caattcatcg
    20101 atatctttag gcgtgatcgt gctagcattc aaactgattt tcaattgatg tgaccagtcg
    20161 gcaatctgct gattggaaat atgataatcc attcttaaac ctgttacacc aattgctttc
    20221 aaaggcgtta gatcttctaa tgaaaaaccc gctcgttcca atgcctctcc tgaaatatcg
    20281 accatcaatt ctagtttatt ctttttcgct tcgcttccta gttcgatcaa tcgcttttga
    20341 tatgccgaaa catcatcttc tggtatatgc atggaagtga aaataccgac aaagccattg
    20401 gatgccattt ggcggatata ctgtttcttc tcttctgaca aagaatcgtt cataaatatt
    20461 gaaaagccaa acatattatc cctctttttc tgtatccgtc ttcatttcta tgactagagt
    20521 atatatctaa agaaacaaaa tttcaatact tttattaaaa taattaaatt tcatttctaa
    20581 aggttgagtt tatattatca aaagaattgt cataacctaa aaaaggcact gctaagcaaa
    20641 agcagtgcct tgaaatattt tatttaaata acacaaaaac ataattactc ataagaaacc
    20701 aattctgtat tatctaccat gaatgtaaag aacccctcat taggcatcgg atgaaaataa
    20761 gcattgatca ctcttatcaa ggcattatgg caaactaaca gatagacatt ttcttcgtct
    20821 tcgatgcatt ctttcaataa aggaacgatc cgtgcataca catctaagac tgactcccca
    20881 tttgggaatc gtacagcgaa cgctaaacgt gctttttgaa agttctcatc tttgcttggc
    20941 agtccatcat aatccccaaa atccatttct accaatcgtt catcaacggt cagcggcaag
    21001 ctgagacgat cagcaactgc ttgtgctgta tctcttgctc gttgaagagg ggaatggatg
    21061 attttcgtga ttggaacagg caaatctact accttttctg ctagtttctg tgcctgcatc
    21121 tgtccttttt cagtcagcgg cacatctgcc cgtccgcaaa ctaatccttg tgcgttccaa
    21181 gttgtttctc cgtgtctcgt tacataaagc atgtgattct ctccttcgtt ggcaaagttc
    21241 cccttaacca taccttgttg ttcttgagat tacaagggtt agattctctc aagagaaata
    21301 atctcagtag atactttatc caggaaggtt tgatcatgtt ccactagtaa catggcaggt
    21361 ttcacgttca agatcagttg ttctaattgt tcttgattga agacatccaa ataattcaat
    21421 ggttcatccc atgtatatag ttcagctggc tgtgataaag atttagccaa ttccactttt
    21481 ttccgttggc ccatactcat ctgctcgatc ttgttatgaa aaacatctct ttccatccca
    21541 agctttcgga ggttgttcaa aaatgcttgg tagtctactt ggtttttctc tgcaaattcc
    21601 gccaacgttc ctcgattgtc ttcataattt tggcttgcat aactaatgct cagatgtttt
    21661 ggctggtatt tttctcctat aaccttgccg ttgaatgccc ccagaagata atggataatg
    21721 gatgactttc ctgcaccgtt aggacctgaa atcgccaccc gctgatgagg ctcgattgta
    21781 aaatgaattg gctcgaataa cagattttca taccctaatt gaagatcttc tacgcttaaa
    21841 agtcgcttat ggtgagacgc ttggctattc atcgtcaaag aatcgatata ttcgatatct
    21901 tttaatagtt tttctttctc gctgatctgg gtctccatcc gattcacgat tgctttggat
    21961 cgtttcatcg tccgtgcagc atcagcaccc actgctcctt tattcactcg tctagattca
    22021 gtatcgatga atccgacttg tttctttgtt ttatctcctt ctcgggaacg agaccattcg
    22081 gctttttcag ctgctgtttt ctttagccta ctgacttctt ttttcaattt ttcattttga
    22141 gccatttcaa attcatcacg aagttttttc tgttcttcat agatagagaa attcccttga
    22201 taaagttcca gttgactttt ttcgattgct aaaacatggt ccactacttc atcgataaat
    22261 ccccggtcat ggctgaccac gatgaagcct tgttttttct ttttcaaata agccgctact
    22321 tgttttctac cagagatatc caaatgattc gttggttcat cgattaacgg gaaatgagtg
    22381 tcatccacaa ataaaagtgc cagtaggact tttgtcttct ctccccccga tagtgtgctg
    22441 aattctctcc ataagatttc aggatctgtt tgcatcaatt ggagctctct ttcgatttcc
    22501 catatctcaa aatccgtaat atcatttaac acgtaatagg ttaaacgttc tttatctttt
    22561 gtctgttgcg ggaaataggc aaattcttgc tgatggatta cttgcccttg ataaggtagt
    22621 ttgttttgta gaatattcag taaagttgtc tttcctcgac cgtttcgtcc gataagtcct
    22681 agtttccatt gtgtgtcaaa atttagattt gcttgttcaa ataataatgt gccttggctg
    22741 tcgtagccga atgtcagatt ttttatttcg attttagaca ttttccatca cctcgtaaaa
    22801 aaagaccagt cctattttca gaactggcta aagaggatgc aagggttgga tacacacaaa
    22861 aagacccata catcgaaaaa accaagccaa tcccgaaaat ctgcacactg cttccttgtc
    22921 tccacaaggt acagtctgaa tgcagctttc atcaatcgac ttaatttttc aatgcacgga
    22981 tccctcactt tgtcctatat ttgaaattac gatagcataa agaaaatgat tttgtcaact
    23041 acttcgggag gatggtgaaa aatctgctaa gtgtctgtcc taagattttt gttttttcta
    23101 atgtttgcct gtgttccatt gcttcttcta aacctattgg acccaaatga atacttaaga
    23161 caattgggag aaaatcagaa agtccttcta gctcttggtc tctacttccg caaatagcga
    23221 ttattggttt tcgatatttt tttgcaagca ctgctacacc gtatggaact tttccgtgga
    23281 tcgtttgctg atcgatccgc ccttctcctg taatgatgac atctgcttgt tcgatctttt
    23341 tttccaatcc agtgatttgg ctgactaaat cgaaaccaga aaccatcttc gctcccaaca
    23401 aaaaaagggc tccaccaata ccgcctgctg ctccacttcc tggaatatca tctaaatcca
    23461 cctcccagaa atcttttatt tgctgagcaa tctttcctgc ttgtttatct aagaaagcta
    23521 gcgtcgtatc tattcctcct ttttgtcttc caaattgcca agcagctcct tgttgacctg
    23581 tatatggatt ggtaacatct gcagcgataa tcaattttac tttagaaaca agaacgtttc
    23641 ttcgatcaaa atgaagtttt ttagtagata ataaaggatt tccttctgtg tacccttcta
    23701 aagaagcacc caaggattgg agtaatccta gaccaccatc gctacacccg cttcctccta
    23761 aagtgacaat aatttgttct acttcttgaa gcagtgcatc tttaataagt tctcctaagg
    23821 cataactgga agcaagtcgg atagtttgat ccgttggttt atcaagtaga tctaacccca
    23881 atgcttgtgc agattcgatg actgcggtgt tttttccttg ccaagttgtc aataaatagc
    23941 tgatttttcg ctgacggaat aagagatcca tcccatcaat ggtacgccat gttcccctga
    24001 gcgcgtaata gattgcttct gcagtatttt ctccgccatc tgcaatcggc acattgattt
    24061 ttcttccttt acgatcttcc cagccattta gaactgcctg atttagttca aatgaagaag
    24121 cacttccttt aaatgaatca attgccgcca aaatatccat ctgtatccct cactttcttc
    24181 ttaagtaaga cacttttttt catttttttc aaacaaaaca
//
LOCUS       DACECS010000036        24036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.036, whole genome shotgun sequence.
ACCESSION   DACECS010000036 DACECS010000000
VERSION     DACECS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.036"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWO31_001572"
     CDS             complement(27..725)
                     /locus_tag="IWO31_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0301986.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWO31_001573"
     CDS             complement(718..2070)
                     /locus_tag="IWO31_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0301987.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWO31_001574"
     CDS             complement(2293..3693)
                     /locus_tag="IWO31_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0301988.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWO31_001575"
     CDS             3854..4591
                     /locus_tag="IWO31_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0301989.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWO31_001576"
     CDS             4716..5195
                     /locus_tag="IWO31_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0301990.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWO31_001577"
     CDS             5192..5959
                     /locus_tag="IWO31_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301991.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWO31_001578"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWO31_001578"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0301992.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWO31_001579"
     CDS             complement(6862..7464)
                     /locus_tag="IWO31_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0301993.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWO31_001580"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWO31_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWO31_001581"
     CDS             8145..8729
                     /locus_tag="IWO31_001581"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0301994.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWO31_001582"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWO31_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0301995.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWO31_001583"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWO31_001583"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0301996.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWO31_001584"
     CDS             10630..10809
                     /locus_tag="IWO31_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0301997.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWO31_001585"
     CDS             10831..11856
                     /locus_tag="IWO31_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0301998.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWO31_001586"
     CDS             12031..13275
                     /locus_tag="IWO31_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0301999.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWO31_001587"
     CDS             13428..14375
                     /locus_tag="IWO31_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0302000.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWO31_001588"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWO31_001588"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0302001.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWO31_001589"
     CDS             complement(15277..15519)
                     /locus_tag="IWO31_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302002.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWO31_001590"
     tRNA            15640..15712
                     /locus_tag="IWO31_001590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IWO31_001591"
     CDS             complement(15831..16469)
                     /locus_tag="IWO31_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0302003.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IWO31_001592"
     CDS             complement(16592..17224)
                     /locus_tag="IWO31_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0302004.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IWO31_001593"
     CDS             17575..17874
                     /locus_tag="IWO31_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0302005.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IWO31_001594"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IWO31_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0302006.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IWO31_001595"
     CDS             complement(20806..21291)
                     /locus_tag="IWO31_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0302007.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IWO31_001596"
     CDS             21771..22412
                     /locus_tag="IWO31_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0302008.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IWO31_001597"
     CDS             22431..23066
                     /locus_tag="IWO31_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302009.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IWO31_001598"
     CDS             23084..23899
                     /locus_tag="IWO31_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0302010.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         8319 a         4090 c         4778 g         6849 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagttt
//
LOCUS       DACECS010000037        23831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.037, whole genome shotgun sequence.
ACCESSION   DACECS010000037 DACECS010000000
VERSION     DACECS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.037"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..356
                     /locus_tag="IWO31_001599"
     CDS             <1..356
                     /locus_tag="IWO31_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0302011.1"
                     /translation="EIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKS
                     LEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGN
                     VFKIEYTKKRQRLAY"
     gene            531..827
                     /locus_tag="IWO31_001600"
     CDS             531..827
                     /locus_tag="IWO31_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302012.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            834..2786
                     /locus_tag="IWO31_001601"
     CDS             834..2786
                     /locus_tag="IWO31_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0302013.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="IWO31_001602"
     CDS             complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="IWO31_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0302014.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3717..4757
                     /locus_tag="IWO31_001603"
     CDS             3717..4757
                     /locus_tag="IWO31_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0302015.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4855..5043
                     /locus_tag="IWO31_001604"
     CDS             4855..5043
                     /locus_tag="IWO31_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302016.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5101..5454)
                     /locus_tag="IWO31_001605"
     CDS             complement(5101..5454)
                     /locus_tag="IWO31_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0302017.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5985..6407
                     /locus_tag="IWO31_001606"
     CDS             5985..6407
                     /locus_tag="IWO31_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0302018.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6404..6634
                     /locus_tag="IWO31_001607"
     CDS             6404..6634
                     /locus_tag="IWO31_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302019.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7132..7332
                     /locus_tag="IWO31_001608"
     CDS             7132..7332
                     /locus_tag="IWO31_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0302020.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7359..8552
                     /locus_tag="IWO31_001609"
     CDS             7359..8552
                     /locus_tag="IWO31_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0302021.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8620..10185)
                     /gene="guaA"
                     /locus_tag="IWO31_001610"
     CDS             complement(8620..10185)
                     /gene="guaA"
                     /locus_tag="IWO31_001610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0302022.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10244..11167)
                     /locus_tag="IWO31_001611"
     CDS             complement(10244..11167)
                     /locus_tag="IWO31_001611"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0302023.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11363..11968
                     /locus_tag="IWO31_001612"
     CDS             11363..11968
                     /locus_tag="IWO31_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0302024.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12276..13292
                     /locus_tag="IWO31_001613"
     CDS             12276..13292
                     /locus_tag="IWO31_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0302025.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13433..14734
                     /locus_tag="IWO31_001614"
     CDS             13433..14734
                     /locus_tag="IWO31_001614"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0302026.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14780..15442
                     /gene="deoC"
                     /locus_tag="IWO31_001615"
     CDS             14780..15442
                     /gene="deoC"
                     /locus_tag="IWO31_001615"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0302027.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15463..15861
                     /locus_tag="IWO31_001616"
     CDS             15463..15861
                     /locus_tag="IWO31_001616"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0302028.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15927..17009
                     /locus_tag="IWO31_001617"
     CDS             15927..17009
                     /locus_tag="IWO31_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0302029.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17221..18783
                     /locus_tag="IWO31_001618"
     CDS             17221..18783
                     /locus_tag="IWO31_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302030.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18776..19909
                     /locus_tag="IWO31_001619"
     CDS             18776..19909
                     /locus_tag="IWO31_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302031.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19909..20865
                     /locus_tag="IWO31_001620"
     CDS             19909..20865
                     /locus_tag="IWO31_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302032.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20964..22130
                     /gene="deoB"
                     /locus_tag="IWO31_001621"
     CDS             20964..22130
                     /gene="deoB"
                     /locus_tag="IWO31_001621"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0302033.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22154..22972
                     /locus_tag="IWO31_001622"
     CDS             22154..22972
                     /locus_tag="IWO31_001622"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0302034.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22989..23696
                     /gene="deoD"
                     /locus_tag="IWO31_001623"
     CDS             22989..23696
                     /gene="deoD"
                     /locus_tag="IWO31_001623"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0302035.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7759 a         4214 c         4975 g         6883 t
ORIGIN      
        1 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
       61 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
      181 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
      241 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      301 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tattaagtag
      361 ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa tgagtatcaa attgcaactg
      421 aatttgatac tcgtttttta ttgttaaatc aacgtttgtt gttaatttac accacaaaat
      481 gtaagttaaa atgtatataa aaatgcgaac gtgaaataat tattgtagga atgcaaattg
      541 gtgtgttaaa atggttacaa tttactaagc aaaaaggggt gcattttcct atgcaatttc
      601 ttgagccaaa aaataagaac gctaagtctg tggattggga aatatcagag caagtaaggg
      661 tgatagttaa gcaatacgct gaatatgcag agcgtactga aagcgaagca gtggatgaat
      721 tcttattgaa cattttagac gataagaagt ttattgaatg gattgccaac aagcgaagta
      781 ataagcggat tgttgaaaaa atgggtatca aagatagagt gggttgatag acaatgacca
      841 agcttaaaag attagccacg aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa
      901 acgaagaatt aaaagatagg gctaaacaat atgatttatt aactccaaaa cgatttgcaa
      961 caaggtataa caaaatgttg ttcttgccaa cgtcttttaa atggaacggt tctgaatatc
     1021 ccattcaata taattactgc atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca
     1081 aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat gactggtagt tccaaagata
     1141 aaggtcataa aggtatgtac tgcaatgaca atcctattgg cagaggtgta tcgcaaaact
     1201 gtacagtaac ccctttatct aattggtctg ttgttgaaga aattaaacgc cttatagaaa
     1261 tcaatagcat ccaagatgtt gagccagatt atcaatttca taaagaaggc tgctcggaag
     1321 aagaatcaac cccattcaat gagccgaagc aattttacaa aagggggaaa agtaggggca
     1381 aatctcaacg ctatcaatgt aaggcgtgta agaagttcac taatgttcta ccaaaaagag
     1441 aagaaaccac tacttatcac caacaaaaga acaccattct tccaatgttc gctaagatgg
     1501 ttgttggaag ggtttcagtc agtcgtactt gtgatatttt aggaattgga gttggaacct
     1561 actatcataa gctggagtgg ctgtataggc gttgtttgga gtttttagaa cgatatgaga
     1621 cacaacccct tcaaacaaag aaatttaacg agatgtggct taatacggac aagatgcact
     1681 actacctaaa taacgtccgt aagaaaggac aaggttcaaa gaaatatacg gggtttgaag
     1741 accttaatat gcaaacgtat atagttgtat ctgctgaggt cttatcaaga tatgtgttcc
     1801 gttctgatgt tgcctatgat tggaatattt caatggatga attaaatgaa gatacaagaa
     1861 aattcaaaga agaccatctc aatactttta gtcgtaaaaa tgataggtta gattggtctt
     1921 attatccaca agaaccatcc gctaacgata gcgaaaatag gaatgcttat cttcacgaat
     1981 tagggaaaat tactaatagg agtagatttg tcgatggttt aaatgttgat gctccttata
     2041 ctacaactgc tcactattgg ttaattaaac aaatggttaa tgccgacgaa tggcgaatga
     2101 ttagtgatga tgacttctct attagaaacg ccttttatag agtattcacg aaagaattaa
     2161 ggttgagcga tgcacatcat tttatttgcc aagttaacaa gacgaaaagc agaaaacaat
     2221 gcttaaaaga attcggacaa gcaaaagcag aactgctcga ttggggagat atcagaggat
     2281 tcaaaacaaa gtttctacgg actttggcat ctcattatct aactgaatta ctaacaagtc
     2341 atcaattcca cgaagaggct attagtaaag atggagaaag atatcgcaaa tatgccgata
     2401 accctatcaa acatccatta gccactaagg ataaaggatt ttattcagtt gattgtagaa
     2461 cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc
     2521 attcgaccaa tagctttatt caacagataa gacgttatat atcatctctt gaaagaccat
     2581 taacaacagc tcgtggtgat aagaagagct atatttacgc taacttcaat ccgaagtatg
     2641 ctcaatttgc gattacaatt ctaagaactt actacaactt ttgtcgccct tttaaatcag
     2701 cggataaaaa ggttttgaca cctgctcaaa gattagggat tacagataaa caatttgatt
     2761 ggaaagatat aatttatttc aaatgacaaa aagagcttgg agtaatccca agctcttgtg
     2821 ctttatcttt actgttcttc ttcaattaac gcacccgtta actcaataag ttttttcttt
     2881 catatacatg aaaatagtag ttatacggat ttttatcatt ctttatccct ttttgggcaa
     2941 atacctcatt ccattcctca taattcactt ctggaaaaaa agtatctcct tcgaattcat
     3001 gatgtatttt tgtgatgtac attttctcaa cataagggaa aaacaaatta taaatctgtt
     3061 ctcctccgaa aataaaaatt tcttcttcgt ttttacataa ctcaaaaaca tcttctattg
     3121 aatgaacaat ttcacaacca ttaaaggtaa accccttatc tctcgtcaga ataatatttc
     3181 ttctgtcagg taaggctctt ccgattgatt caaggttctt cctacctaat attatcggat
     3241 gtccctttgt agtattttta acatattccc agtccttggg aatcctccaa ggaatgtcat
     3301 tctctttgcc aatcactcta ttcttatcca tcgcagcaat caaagaaact ttcatttata
     3361 acacagcctt tttaactaat ttaccatcta atttcttatt tctatcaaaa aaccttttct
     3421 cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt atcataatac
     3481 cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata aatcattttg
     3541 tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag tacattttgt
     3601 cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat agtggagtac
     3661 tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt
     3721 atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta
     3781 ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac
     3841 agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac
     3901 ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc
     3961 tacgatatta cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac
     4021 aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag
     4081 agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg
     4141 aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg
     4201 gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa
     4261 atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt tggaatgtga
     4321 cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt actccagcag
     4381 attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct ggaacagaat
     4441 tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt tcacgggcat
     4501 ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg
     4561 tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat gatttaactt
     4621 tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag gttaccactg
     4681 gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta agatatatgt
     4741 tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct tattaaataa
     4801 gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat tgttattata
     4861 aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg aatacgagtg
     4921 gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga aactttaatt
     4981 aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac gcagagccga
     5041 taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga attttactga
     5101 ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca ttaaggatag
     5161 ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag tgcttttaat
     5221 caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg atacaagatc
     5281 atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta aataacgtgg
     5341 gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca ttcgagcctc
     5401 tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac gcatactaaa
     5461 tcacctctat acattttact gttcctatat aaccataaac aggtatagaa aaacatgtta
     5521 tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga atccaatttc
     5581 aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta gtcatactcc
     5641 tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag aaagataatc
     5701 tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct gtactggata
     5761 gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa gcatatgaac
     5821 agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt ttaggatttc
     5881 tcttcattta tgcaacaaat cccccgttcg tgtcggggtt tagtatgaag ggagataaag
     5941 aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa ccatcttctt
     6001 ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt gaagatgaac
     6061 gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg ttttctgata
     6121 tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact gatttccaac
     6181 tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt gaagccctgc
     6241 gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg gatatgagtg
     6301 atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt catcgaataa
     6361 gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa cagcttatcc
     6421 aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag caattaatca
     6481 gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca tgacagacga
     6541 atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg aaacgaaact
     6601 catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg aagcgtgtcg
     6661 aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca tattgtgtgt
     6721 tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat aaaggcaatc
     6781 gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta caaaattagt
     6841 gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc atgactttct
     6901 tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg tcacaggcaa
     6961 tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt gctagagctg
     7021 ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat ttgatataat
     7081 tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat aatgataacg
     7141 gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc agacatcata
     7201 aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc tcgaaagcta
     7261 gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc aaatgaaaac
     7321 aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac ccaataaaaa
     7381 aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct aaagggaaaa
     7441 aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa gaatattcga
     7501 aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg acgtttaaac
     7561 attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc agaacatatg
     7621 aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg gctgtttccg
     7681 atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag tgtaagtctt
     7741 cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca attgtccttg
     7801 ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa aagtctaaaa
     7861 ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac aaagaagatt
     7921 actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga atgcgaattg
     7981 gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta ctaaccatca
     8041 acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca aaaacaaagg
     8101 cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc gaatggaagg
     8161 aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt attcctaccc
     8221 aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt atccatcgaa
     8281 tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg ggagaaaatc
     8341 cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta ggaacgtatg
     8401 gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga gccatttcct
     8461 ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact gtagactact
     8521 tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata agaagagcca
     8581 gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact ccactgttgc
     8641 tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat
     8701 acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc
     8761 gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat
     8821 aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag
     8881 tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt
     8941 gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg
     9001 ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa
     9061 taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt
     9121 atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa
     9181 cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc
     9241 aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc
     9301 tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca agccgaattt
     9361 tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac
     9421 aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga
     9481 atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt
     9541 ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc
     9601 aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa
     9661 ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct
     9721 tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca
     9781 tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa
     9841 caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt
     9901 gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg
     9961 atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc
    10021 ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa
    10081 gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa
    10141 tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat
    10201 ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga
    10261 aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg
    10321 taggcaagat atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca
    10381 ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt
    10441 ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg
    10501 gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg
    10561 caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt
    10621 cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt
    10681 cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag
    10741 gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc
    10801 cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca
    10861 gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag
    10921 gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt
    10981 ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca
    11041 tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt
    11101 gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat
    11161 cattcatatt agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg
    11221 caaaccccac cacttaagag agattgccga tctttttgta taatctttat aatagaaaga
    11281 aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa
    11341 agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc
    11401 gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga
    11461 ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt
    11521 cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc
    11581 gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga
    11641 acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat
    11701 ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa
    11761 tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg
    11821 gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc
    11881 gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata
    11941 ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt
    12001 ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa
    12061 aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg
    12121 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
    12181 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
    12241 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
    12301 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
    12361 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
    12421 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
    12481 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
    12541 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
    12601 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
    12661 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
    12721 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
    12781 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
    12841 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
    12901 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
    12961 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
    13021 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
    13081 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
    13141 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
    13201 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
    13261 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
    13321 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
    13381 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
    13441 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
    13501 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
    13561 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
    13621 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
    13681 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
    13741 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
    13801 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
    13861 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
    13921 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
    13981 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
    14041 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
    14101 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
    14161 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
    14221 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
    14281 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
    14341 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
    14401 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
    14461 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
    14521 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
    14581 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
    14641 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
    14701 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
    14761 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
    14821 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
    14881 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
    14941 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
    15001 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
    15061 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
    15121 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
    15181 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
    15241 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
    15301 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
    15361 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
    15421 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
    15481 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
    15541 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
    15601 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
    15661 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
    15721 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
    15781 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
    15841 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
    15901 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
    15961 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
    16021 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
    16081 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
    16141 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
    16201 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
    16261 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
    16321 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
    16381 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
    16441 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
    16501 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
    16561 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
    16621 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
    16681 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
    16741 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
    16801 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
    16861 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
    16921 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
    16981 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
    17041 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
    17101 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
    17161 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
    17221 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
    17281 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
    17341 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
    17401 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
    17461 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
    17521 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
    17581 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
    17641 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
    17701 ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat
    17761 gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg
    17821 cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa
    17881 ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt
    17941 ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg
    18001 tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac
    18061 ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg
    18121 gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa
    18181 ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg
    18241 ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca
    18301 ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg
    18361 aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt
    18421 cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac
    18481 cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc
    18541 gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc
    18601 agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga
    18661 gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg
    18721 gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa
    18781 tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt
    18841 gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc
    18901 tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac
    18961 gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat
    19021 tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt
    19081 tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg
    19141 tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat
    19201 cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt
    19261 gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc
    19321 actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt
    19381 tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt
    19441 tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa
    19501 acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt
    19561 tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt
    19621 tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc
    19681 gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc
    19741 gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct
    19801 gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac
    19861 aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc
    19921 gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg
    19981 tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc
    20041 atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt
    20101 tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg
    20161 attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt
    20221 aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa
    20281 actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg
    20341 gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt
    20401 gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt
    20461 ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca
    20521 ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt
    20581 tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg
    20641 atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct
    20701 caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg
    20761 ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct
    20821 tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa
    20881 aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa
    20941 ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag
    21001 taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct gatacattag
    21061 gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa
    21121 cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat
    21181 tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga
    21241 atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa
    21301 tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta
    21361 ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg
    21421 atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga
    21481 tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg
    21541 cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg
    21601 cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga
    21661 ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca
    21721 cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag
    21781 gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag
    21841 tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga
    21901 tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg
    21961 gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac
    22021 aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta
    22081 gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa
    22141 taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct
    22201 tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt
    22261 agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt
    22321 ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt
    22381 gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta
    22441 tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg
    22501 tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt
    22561 tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga
    22621 tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact
    22681 aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc
    22741 tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc
    22801 agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa
    22861 cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag
    22921 agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt aataggagga
    22981 acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac
    23041 ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt
    23101 gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt
    23161 ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc
    23221 agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg
    23281 tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa
    23341 aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat
    23401 atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt
    23461 ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag
    23521 tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa
    23581 gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc
    23641 aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag
    23701 gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca
    23761 tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa
    23821 gaggttgtga a
//
LOCUS       DACECS010000038        23649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.038, whole genome shotgun sequence.
ACCESSION   DACECS010000038 DACECS010000000
VERSION     DACECS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.038"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(56..790)
                     /locus_tag="IWO31_001624"
     CDS             complement(56..790)
                     /locus_tag="IWO31_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0302036.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="IWO31_001625"
     CDS             1117..1527
                     /locus_tag="IWO31_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0302037.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="IWO31_001626"
     CDS             1520..2221
                     /locus_tag="IWO31_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0302038.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="IWO31_001627"
     CDS             2221..3807
                     /locus_tag="IWO31_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0302039.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="IWO31_001628"
     CDS             3826..4047
                     /locus_tag="IWO31_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0302040.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="IWO31_001629"
     CDS             4044..4805
                     /locus_tag="IWO31_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0302041.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="IWO31_001630"
     CDS             5185..5694
                     /locus_tag="IWO31_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0302042.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="IWO31_001631"
     CDS             complement(5769..6308)
                     /locus_tag="IWO31_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0302043.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IWO31_001632"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IWO31_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0302044.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7375..8172
                     /locus_tag="IWO31_001633"
     CDS             7375..8172
                     /locus_tag="IWO31_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0302045.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8200..8835
                     /locus_tag="IWO31_001634"
     CDS             8200..8835
                     /locus_tag="IWO31_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0302046.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="IWO31_001635"
     CDS             8855..9628
                     /locus_tag="IWO31_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0302047.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="IWO31_001636"
     CDS             10036..10833
                     /locus_tag="IWO31_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0302048.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="IWO31_001637"
     CDS             10811..11224
                     /locus_tag="IWO31_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302049.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="IWO31_001638"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="IWO31_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0302050.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="IWO31_001639"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="IWO31_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0302051.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="IWO31_001640"
     CDS             16001..16561
                     /locus_tag="IWO31_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0302052.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IWO31_001641"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IWO31_001641"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0302053.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="IWO31_001642"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="IWO31_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0302054.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="IWO31_001643"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="IWO31_001643"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0302055.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="IWO31_001644"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="IWO31_001644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0302056.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="IWO31_001645"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="IWO31_001645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0302057.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="IWO31_001646"
     CDS             21908..22486
                     /locus_tag="IWO31_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0302058.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="IWO31_001647"
     CDS             22538..23020
                     /locus_tag="IWO31_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0302059.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="IWO31_001648"
     CDS             23137..23511
                     /locus_tag="IWO31_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302060.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7729 a         4272 c         5066 g         6582 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatagaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc atgacagcat
    23641 tcggttgat
//
LOCUS       DACECS010000039        23058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.039, whole genome shotgun sequence.
ACCESSION   DACECS010000039 DACECS010000000
VERSION     DACECS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.039"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWO31_001649"
     CDS             complement(109..564)
                     /locus_tag="IWO31_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302061.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWO31_001650"
     CDS             complement(718..1926)
                     /locus_tag="IWO31_001650"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0302062.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWO31_001651"
     CDS             complement(2038..2370)
                     /locus_tag="IWO31_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0302063.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWO31_001652"
     CDS             2518..3393
                     /locus_tag="IWO31_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0302064.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWO31_001653"
     CDS             complement(3475..4056)
                     /locus_tag="IWO31_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0302065.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWO31_001654"
     CDS             complement(4062..5321)
                     /locus_tag="IWO31_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0302066.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWO31_001655"
     CDS             complement(5323..6369)
                     /locus_tag="IWO31_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302067.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWO31_001656"
     CDS             complement(6604..6879)
                     /locus_tag="IWO31_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0302068.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWO31_001657"
     CDS             complement(7122..8432)
                     /locus_tag="IWO31_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0302069.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO31_001658"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO31_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0302070.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWO31_001659"
     CDS             complement(9978..10658)
                     /locus_tag="IWO31_001659"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0302071.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWO31_001660"
     CDS             complement(10707..11342)
                     /locus_tag="IWO31_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0302072.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWO31_001661"
     CDS             complement(11408..12811)
                     /locus_tag="IWO31_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0302073.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWO31_001662"
     CDS             complement(12798..13829)
                     /locus_tag="IWO31_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302074.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWO31_001663"
     CDS             13860..14081
                     /locus_tag="IWO31_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0302075.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWO31_001664"
     CDS             complement(14246..14983)
                     /locus_tag="IWO31_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0302076.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWO31_001665"
     CDS             complement(15136..16476)
                     /locus_tag="IWO31_001665"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0302077.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWO31_001666"
     CDS             complement(16713..18236)
                     /locus_tag="IWO31_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0302078.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWO31_001667"
     CDS             complement(18614..19225)
                     /locus_tag="IWO31_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0302079.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWO31_001668"
     CDS             complement(19555..20271)
                     /locus_tag="IWO31_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0302080.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO31_001669"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO31_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0302081.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWO31_001670"
     CDS             complement(20830..21627)
                     /locus_tag="IWO31_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0302082.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWO31_001671"
     CDS             complement(21597..21968)
                     /locus_tag="IWO31_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0302083.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO31_001672"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO31_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0302084.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6463 a         4926 c         3910 g         7759 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggta
//
LOCUS       DACECS010000040        22457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.040, whole genome shotgun sequence.
ACCESSION   DACECS010000040 DACECS010000000
VERSION     DACECS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.040"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWO31_001673"
     CDS             <1..152
                     /locus_tag="IWO31_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302085.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWO31_001674"
     CDS             complement(230..763)
                     /locus_tag="IWO31_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0302086.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWO31_001675"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWO31_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0302087.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWO31_001676"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWO31_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0302088.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWO31_001677"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWO31_001677"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0302089.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWO31_001678"
     CDS             complement(4488..5066)
                     /locus_tag="IWO31_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0302090.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWO31_001679"
     CDS             5388..5858
                     /locus_tag="IWO31_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0302091.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWO31_001680"
     CDS             complement(5966..7192)
                     /locus_tag="IWO31_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0302092.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IWO31_001681"
     CDS             complement(7946..8146)
                     /locus_tag="IWO31_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0302093.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IWO31_001682"
     CDS             complement(8280..8888)
                     /locus_tag="IWO31_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0302094.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IWO31_001683"
     CDS             complement(8973..9443)
                     /locus_tag="IWO31_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0302095.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IWO31_001684"
     CDS             complement(9491..10405)
                     /locus_tag="IWO31_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0302096.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IWO31_001685"
     CDS             10551..11354
                     /locus_tag="IWO31_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0302097.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWO31_001686"
     CDS             11532..12479
                     /locus_tag="IWO31_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0302098.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWO31_001687"
     CDS             complement(12547..12840)
                     /locus_tag="IWO31_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0302099.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWO31_001688"
     CDS             complement(12868..13533)
                     /locus_tag="IWO31_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0302100.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWO31_001689"
     CDS             complement(13673..14305)
                     /locus_tag="IWO31_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302101.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWO31_001690"
     CDS             complement(14312..15379)
                     /locus_tag="IWO31_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0302102.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWO31_001691"
     CDS             complement(15376..16107)
                     /locus_tag="IWO31_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0302103.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWO31_001692"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWO31_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0302104.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWO31_001693"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWO31_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0302105.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWO31_001694"
     CDS             18270..19439
                     /locus_tag="IWO31_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0302106.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21328)
                     /locus_tag="IWO31_001695"
     CDS             complement(19706..21328)
                     /locus_tag="IWO31_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302107.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            21825..22457
                     /locus_tag="IWO31_001696"
                     /pseudo
     CDS             21825..22457
                     /locus_tag="IWO31_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         6971 a         4369 c         3556 g         7561 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat attacatata
    21361 gtatcattta tttttttaaa acactagatt actcactata taaaatgtga ttaattttat
    21421 tattaataaa atatttttta tattacaaaa gtagtgtttc aaagttagta gagaaaatgt
    21481 agttatatat ttaaattttt accagaaaga aatgaacacc ttcaattatc taaatctcga
    21541 aaatggttag atgtaatcat caggaaaaac aatcttaaac gaattactat tcatggtctt
    21601 agacacacac atgctagctt gcttttagca agctagtgca aatattaaag atgtgcaaga
    21661 gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata ttacagacaa
    21721 aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt tatgggactg
    21781 atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt cgtacattga
    21841 aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa gtattgatgg
    21901 tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt tgtgcggatg
    21961 gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga ttaacacact
    22021 attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat atgaacgccg
    22081 cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt gattgttttc
    22141 atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat
    22201 tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc
    22261 taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac
    22321 ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc
    22381 ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat
    22441 cagacttatt ttttacc
//
LOCUS       DACECS010000041        22028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.041, whole genome shotgun sequence.
ACCESSION   DACECS010000041 DACECS010000000
VERSION     DACECS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.041"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWO31_001697"
     CDS             complement(61..1371)
                     /locus_tag="IWO31_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0302108.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWO31_001698"
     CDS             complement(1383..2801)
                     /locus_tag="IWO31_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0302109.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWO31_001699"
     CDS             complement(3041..4609)
                     /locus_tag="IWO31_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0302110.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWO31_001700"
     CDS             complement(4613..4894)
                     /locus_tag="IWO31_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0302111.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWO31_001701"
     CDS             complement(4891..5814)
                     /locus_tag="IWO31_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0302112.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWO31_001702"
     CDS             complement(5811..6689)
                     /locus_tag="IWO31_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0302113.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWO31_001703"
     CDS             complement(6676..8013)
                     /locus_tag="IWO31_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0302114.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWO31_001704"
     CDS             complement(8016..9119)
                     /locus_tag="IWO31_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0302115.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWO31_001705"
     CDS             complement(9193..10701)
                     /locus_tag="IWO31_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0302116.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWO31_001706"
     CDS             complement(10667..11359)
                     /locus_tag="IWO31_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0302117.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWO31_001707"
     CDS             complement(11381..12355)
                     /locus_tag="IWO31_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0302118.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWO31_001708"
     CDS             12655..13527
                     /locus_tag="IWO31_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0302119.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWO31_001709"
     CDS             complement(14109..16307)
                     /locus_tag="IWO31_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302120.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWO31_001710"
     CDS             16539..17531
                     /locus_tag="IWO31_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0302121.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWO31_001711"
     CDS             17591..19012
                     /locus_tag="IWO31_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0302122.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWO31_001712"
     CDS             19005..20504
                     /locus_tag="IWO31_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0302123.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWO31_001713"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWO31_001713"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0302124.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DACECS010000042        21988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.042, whole genome shotgun sequence.
ACCESSION   DACECS010000042 DACECS010000000
VERSION     DACECS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.042"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWO31_001714"
     CDS             <1..395
                     /locus_tag="IWO31_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302125.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IWO31_001715"
     CDS             398..1147
                     /locus_tag="IWO31_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302126.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IWO31_001716"
     CDS             1159..1428
                     /locus_tag="IWO31_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302127.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IWO31_001717"
     CDS             1590..1892
                     /locus_tag="IWO31_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302128.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IWO31_001718"
     CDS             1889..2050
                     /locus_tag="IWO31_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0302129.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IWO31_001719"
     CDS             2047..2352
                     /locus_tag="IWO31_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302130.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IWO31_001720"
     CDS             2352..2708
                     /locus_tag="IWO31_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0302131.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IWO31_001721"
     CDS             2668..2913
                     /locus_tag="IWO31_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302132.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IWO31_001722"
     CDS             2910..3329
                     /locus_tag="IWO31_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0302133.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IWO31_001723"
     CDS             3326..3883
                     /locus_tag="IWO31_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0302134.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IWO31_001724"
     CDS             3880..4176
                     /locus_tag="IWO31_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302135.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IWO31_001725"
     CDS             4253..4666
                     /locus_tag="IWO31_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0302136.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="IWO31_001726"
     CDS             5012..5386
                     /locus_tag="IWO31_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302137.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="IWO31_001727"
     CDS             5616..5960
                     /locus_tag="IWO31_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0302138.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="IWO31_001728"
     CDS             5965..6246
                     /locus_tag="IWO31_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302139.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="IWO31_001729"
     CDS             6349..6663
                     /locus_tag="IWO31_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0302140.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="IWO31_001730"
     CDS             6641..8335
                     /locus_tag="IWO31_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0302141.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="IWO31_001731"
     CDS             8355..9533
                     /locus_tag="IWO31_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0302142.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="IWO31_001732"
     CDS             9496..10182
                     /locus_tag="IWO31_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0302143.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="IWO31_001733"
     CDS             10182..11342
                     /locus_tag="IWO31_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0302144.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="IWO31_001734"
     CDS             11352..12227
                     /locus_tag="IWO31_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302145.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="IWO31_001735"
     CDS             12224..12535
                     /locus_tag="IWO31_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302146.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="IWO31_001736"
     CDS             12525..12878
                     /locus_tag="IWO31_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0302147.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="IWO31_001737"
     CDS             12868..13269
                     /locus_tag="IWO31_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302148.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="IWO31_001738"
     CDS             13262..13666
                     /locus_tag="IWO31_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302149.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="IWO31_001739"
     CDS             13678..14286
                     /locus_tag="IWO31_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0302150.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="IWO31_001740"
     CDS             14306..14668
                     /locus_tag="IWO31_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302151.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="IWO31_001741"
     CDS             14671..14853
                     /locus_tag="IWO31_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302152.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="IWO31_001742"
     CDS             14870..18289
                     /locus_tag="IWO31_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0302153.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="IWO31_001743"
     CDS             18340..19077
                     /locus_tag="IWO31_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0302154.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="IWO31_001744"
     CDS             19087..21378
                     /locus_tag="IWO31_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302155.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="IWO31_001745"
     CDS             21402..>21988
                     /locus_tag="IWO31_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302156.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DACECS010000043        21911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.043, whole genome shotgun sequence.
ACCESSION   DACECS010000043 DACECS010000000
VERSION     DACECS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.043"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO31_001746"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO31_001746"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0302157.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO31_001747"
     CDS             complement(1172..1867)
                     /locus_tag="IWO31_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0302158.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO31_001748"
     CDS             complement(1880..2596)
                     /locus_tag="IWO31_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0302159.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO31_001749"
     CDS             2926..3534
                     /locus_tag="IWO31_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0302160.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO31_001750"
     CDS             3859..4182
                     /locus_tag="IWO31_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0302161.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO31_001751"
     CDS             4364..5734
                     /locus_tag="IWO31_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0302162.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO31_001752"
     CDS             complement(5864..7165)
                     /locus_tag="IWO31_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0302163.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO31_001753"
     CDS             complement(7519..9510)
                     /locus_tag="IWO31_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0302164.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO31_001754"
     CDS             complement(9654..9920)
                     /locus_tag="IWO31_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0302165.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO31_001755"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO31_001755"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0302166.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO31_001756"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO31_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0302167.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWO31_001757"
     CDS             13264..13827
                     /locus_tag="IWO31_001757"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0302168.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWO31_001758"
     CDS             complement(14080..14601)
                     /locus_tag="IWO31_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0302169.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWO31_001759"
     CDS             complement(14604..15446)
                     /locus_tag="IWO31_001759"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0302170.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWO31_001760"
     CDS             complement(15439..16164)
                     /locus_tag="IWO31_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0302171.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWO31_001761"
     CDS             16340..16636
                     /locus_tag="IWO31_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302172.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17203..18795
                     /locus_tag="IWO31_001762"
     CDS             17203..18795
                     /locus_tag="IWO31_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0302173.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="IWO31_001763"
     CDS             18808..19548
                     /locus_tag="IWO31_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302174.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="IWO31_001764"
     CDS             19716..21065
                     /locus_tag="IWO31_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0302175.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="IWO31_001765"
     CDS             complement(21193..21783)
                     /locus_tag="IWO31_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0302176.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6996 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aataactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accatcgttt tatataggga attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa a
//
LOCUS       DACECS010000044        21671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.044, whole genome shotgun sequence.
ACCESSION   DACECS010000044 DACECS010000000
VERSION     DACECS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.044"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..579
                     /locus_tag="IWO31_001766"
     CDS             <1..579
                     /locus_tag="IWO31_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0302177.1"
                     /translation="GRAFTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHR
                     YWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKE
                     LPEHYQPVFTTFKKYQTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRER
                     IFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(820..2436)
                     /locus_tag="IWO31_001767"
     CDS             complement(820..2436)
                     /locus_tag="IWO31_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0302178.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2554..2772
                     /locus_tag="IWO31_001768"
     CDS             2554..2772
                     /locus_tag="IWO31_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302179.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            2967..3428
                     /locus_tag="IWO31_001769"
     CDS             2967..3428
                     /locus_tag="IWO31_001769"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0302180.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3621..3860)
                     /locus_tag="IWO31_001770"
     CDS             complement(3621..3860)
                     /locus_tag="IWO31_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302181.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3884..5407)
                     /locus_tag="IWO31_001771"
     CDS             complement(3884..5407)
                     /locus_tag="IWO31_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0302182.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5425..5547)
                     /locus_tag="IWO31_001772"
     CDS             complement(5425..5547)
                     /locus_tag="IWO31_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0302183.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5577..6560)
                     /locus_tag="IWO31_001773"
     CDS             complement(5577..6560)
                     /locus_tag="IWO31_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0302184.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6584..6733)
                     /gene="rpmG"
                     /locus_tag="IWO31_001774"
     CDS             complement(6584..6733)
                     /gene="rpmG"
                     /locus_tag="IWO31_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0302185.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6754..7131)
                     /locus_tag="IWO31_001775"
     CDS             complement(6754..7131)
                     /locus_tag="IWO31_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302186.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7163..7342)
                     /gene="rpmF"
                     /locus_tag="IWO31_001776"
     CDS             complement(7163..7342)
                     /gene="rpmF"
                     /locus_tag="IWO31_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0302187.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7357..7626)
                     /gene="rpsN"
                     /locus_tag="IWO31_001777"
     CDS             complement(7357..7626)
                     /gene="rpsN"
                     /locus_tag="IWO31_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0302188.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8149..9921)
                     /locus_tag="IWO31_001778"
     CDS             complement(8149..9921)
                     /locus_tag="IWO31_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302189.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9875..11629)
                     /locus_tag="IWO31_001779"
     CDS             complement(9875..11629)
                     /locus_tag="IWO31_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302190.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11739..12308)
                     /locus_tag="IWO31_001780"
     CDS             complement(11739..12308)
                     /locus_tag="IWO31_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0302191.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12326..13750)
                     /locus_tag="IWO31_001781"
     CDS             complement(12326..13750)
                     /locus_tag="IWO31_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0302192.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13752..14420)
                     /locus_tag="IWO31_001782"
     CDS             complement(13752..14420)
                     /locus_tag="IWO31_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0302193.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14550..15134)
                     /locus_tag="IWO31_001783"
     CDS             complement(14550..15134)
                     /locus_tag="IWO31_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0302194.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15465..15698)
                     /locus_tag="IWO31_001784"
     CDS             complement(15465..15698)
                     /locus_tag="IWO31_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0302195.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15713..16663)
                     /locus_tag="IWO31_001785"
     CDS             complement(15713..16663)
                     /locus_tag="IWO31_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0302196.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16660..17502)
                     /locus_tag="IWO31_001786"
     CDS             complement(16660..17502)
                     /locus_tag="IWO31_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0302197.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17504..18223)
                     /locus_tag="IWO31_001787"
     CDS             complement(17504..18223)
                     /locus_tag="IWO31_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302198.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            18915..19073
                     /locus_tag="IWO31_001788"
     CDS             18915..19073
                     /locus_tag="IWO31_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302199.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            19145..20371
                     /locus_tag="IWO31_001789"
     CDS             19145..20371
                     /locus_tag="IWO31_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0302200.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20459..20851)
                     /gene="rpsI"
                     /locus_tag="IWO31_001790"
     CDS             complement(20459..20851)
                     /gene="rpsI"
                     /locus_tag="IWO31_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0302201.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(20867..21310)
                     /gene="rplM"
                     /locus_tag="IWO31_001791"
     CDS             complement(20867..21310)
                     /gene="rplM"
                     /locus_tag="IWO31_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0302202.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         7082 a         4036 c         3124 g         7429 t
ORIGIN      
        1 ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt
       61 cggtcgaagc acttaaagaa atactggaaa ctacttcaaa agaatgcgtg ggaattgaaa
      121 ggtcagcatc gctactggcg tccgtcattt cgagatcatc tgacagaagc agagatagtc
      181 gatcggctat tatcttatga tgattcctta aagcgaggct acgaagtcta ccaggatttt
      241 ctttccgtta tcagaagaca agatgttcct gaattcgtcg cgctcctaaa agaagactac
      301 aaggagttgc cagagcacta ccaaccagtg ttcacgacct tcaagaaata ccaaacagaa
      361 atcaaacgag cgttgcgcgt tccctattcc aacggcccta tagaatgttt gaataaccat
      421 atcaaagtac taaaacgaat tgcctatggc tatagaaact ttcaaaacta ccgagaacga
      481 atctttttat atcgaggaaa atattttaag aaaactaaga atactaccca actgactaaa
      541 gccagaacaa caacacggct agacaagata gctgtctagc cgtgtgagta tctcgattat
      601 tctattgaat ttcttcacca acactcattg acgaagagcc aaaataagta atctaaccga
      661 ctgttaacta aaccattgca ggtcccgtta aagatacttg tgcctgttgt caccgcttct
      721 ttttcttgtc cacaagcgct taacacactt aataaagccc ctgctattat taataataat
      781 atctttttct tattaaaaac ctcctcatct atttccagat tagtaaatgt agtaatctgt
      841 gctttctttt ttctcaatac taataactaa cttatggggt aaacaaatac tcgtttgacc
      901 gggttttgag atccatccag ttctaaccgc gatttgatca ggactattat cttctttaac
      961 tcgaattctt ccatctttaa tctcaacaat attatattgt cccttcgcag gatgataatt
     1021 cacgagttta tgattgatct tatccaaatc gaagcgatcc atttccttgc catcaattcg
     1081 aacgatggca tatttgatag catcttcagt tgtttcattc tccttttcta tagagaatac
     1141 aaataaggga ataaaagagg taataatcaa aatgccaata attatgacat cgcctatctt
     1201 gacaccagct tttcttaaaa atttcattaa cgatatctgg ctaaacatat tagacttttt
     1261 ccatggaaat ggtgaaattt ttcggaaatt atctgcatta agagaatcag ttaaacaacc
     1321 ctcaagttgt tccttaatac attgttgatc catcttcata ccaataaaaa ctagtttatt
     1381 aatgcgttca ccaaacaacg gatgccaggc ttccttcaat tcgggatttt tttgtataat
     1441 catttgacac tcaattttag gtaaactagc tacccaataa tttaatgatt caatactttt
     1501 atagctaccc gatgtttcaa agatcgtcga aaaatcacta tgttgggcaa accaaagaaa
     1561 cccttttgct cttgtaatat tgccaggaaa atttttatcc gtaagatggg caaatttttg
     1621 tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag ataccatatt cttctgtttc
     1681 aggaatatga ttttgatagc caagctctaa ctctttgaac cagcccactg aataggaagt
     1741 ctcatctata ttaaaaagat ttgtatttag aattttcgat aagtctactt cacctttaat
     1801 tgtcctaatt attttggcat tggactgtaa cttagcaatt agaccgttaa ttttgtaaat
     1861 cgtcttggtt gacactaagt cactattatt taaaagtaat atgttacaac actcgaattg
     1921 atcaattaac aagccacgaa tatcctgttc ttcttcaact acattacctt cagcttttct
     1981 ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa cgttttacat ccactactat
     2041 aataatggca ccaatctcta atcgttgggt caaatcaatg tctaaagggt ttttagctaa
     2101 tataattgct tgaataattg gaattggttt gctgattcct gaagcttcaa taacaatttg
     2161 attcaagtca gaaaattgca ctaattgatc tagttcaaca atcaaatctt cgcgtaatgt
     2221 acaacaaatg catccattcg gcatcgaaac aagtttttct ccagttcttt taaaaccttt
     2281 cttgtcgact aataccccat caacatttac ttcccctatg tcatttacaa tgacagcaat
     2341 ttcctttccg tgatcaccgt acagaatatg atttagaagt atcgtttttc ctgaaccaag
     2401 atagccagtt aagattgtaa taggtatttt tttcataggg aacctctttc tataccttga
     2461 atttacactt taagtgcaac acataaaaat gagtcataat cgatatccaa ctagtataag
     2521 tttgtcgaga acactcctag aaggaaaata attatgagcc agtatcgtca tcttactata
     2581 ctcgaacgag aaagaatctt tctgcttcac gaagaaggat tttctattag tataattggc
     2641 aacgacattc agcatagccc gtttactatt ttacatgaac tgagaagaaa caagaaaaaa
     2701 actattcacc ttccaaagcg gaaaaaaatt atgcttatcg aagaaaaaaa tgcggacgtt
     2761 atcacattct aaatgatccg tgagtttggt caattgttcg tcatttattt gtcgattttc
     2821 agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga acatatggac ttttcaatta
     2881 gttttatcac gatctaacgt gcaatttgca atgatctttt cgatacagaa cccctctcac
     2941 acggcaataa aggcctgatc cgttcattgc accatcgagg aaaaacaaga catacaaaaa
     3001 atcatgtcga gtctcgtgga aagatttgcc ttactcattc gatccatggc cgtccaaaat
     3061 ccgcagatga tagaagtgat tttggtcatt ggaaagccga tattgtagct ggcaaaactg
     3121 gtggtacttg tttagtgact ttgacagaca gaaaatcatg cttcctttta gcaggaaaga
     3181 aggtccccag aaagaaagcc gcttatgtga gcgagaaaat gattgaactt ttaagcagta
     3241 ttccaaatga ggcggtcaaa acgatcactc cagatagagg aaaagaattc tccaatcatt
     3301 tcgcagttac agaagcattg aataatatgc ccttttattt tccagatcct catgcccttg
     3361 gcaacaatga acaaattaaa atacgaatag actcattcga gaatacttgc caaaatcgaa
     3421 agagatagct acggtagata actcaacgat aaatcagatg attgaaaaac tgaataacag
     3481 acctagaaag tgtttagatt ggagaacacc ttatgaagtc ttctttgata aagtgttgcg
     3541 cttgatttga caattcaaga cataataaaa agcagatcaa atccaaataa gggaaaaatg
     3601 atctactctg gtattatcaa ttaattggcc aagctattat gccgttttgc taaacgtcta
     3661 cttcttccag tattgacctt gataaaacaa caatcaggat cccgttttaa tactctatga
     3721 actgtgtcaa tactaacatg taaagttgca gcaatatcag ctaatgtact cccatttaat
     3781 ctcatttgct ttgcaaatcg ataacgccga taagcatcaa tattcttgga atccatagac
     3841 aataatactt ttcttaccat gtatttcctc caaaaattga attttaatga gccatcattt
     3901 cttgagcaat ttcatgttta gacaatgaag ctggatagaa agtaggccaa ttatgcaatt
     3961 cattatagag tttttcttga ctttctccac caaagaaaat atggaaatgc tctgccttac
     4021 ttggtgcaat tccatgatca ctaaattgaa tatatttaaa acgaccagca cctgcatcat
     4081 cagtttcaaa attaaaacga acaccacggt ttcctttttc ataattcaaa attttgtatc
     4141 caacatattt gtatgtgtat tgatgatgtt caccgttgac tacaaaatca atggtgttgt
     4201 ctttaatgtt gatagtatca atatctgtct tatagccagt agtataataa tctttgtact
     4261 cggcagctgt catgtcttta ttgatttttg ctttgtaatc gaaaacttca tccaaaattc
     4321 catcttttaa caacggataa acagattgcc attcgcctgt ataatcactc aattcgcgat
     4381 ccttcacatc ggcatcttta aagtacccat tagcaactgt cttttcatcc ttcgcttctt
     4441 tttctggaac tacctcttca ccagcaacca tagttgtttt ttctaacgct tttagattgt
     4501 cttccattac tgaaacataa tcttcaccat tatccatttg ttcttttgta agactttcaa
     4561 gaggattcag tacttccaat tttatcccag cttcgttggc caaagttttt gcaattttat
     4621 cattagcatt cttttcaaag taaatataat tgatcttatt ttttttaaca tactctttta
     4681 attcagctaa tcgaccagaa gatggctctt cttcaggact tagtccagca ataggaactt
     4741 gtttcaatcc ataatccaaa gctaaatatc caaaggctgc gtgttgcgtc acaaaacttt
     4801 tttgtttcgc ttctttaaga ctagtggtat agtcagcatc caaccgtttc agtttagtca
     4861 aatacttttc agcatttgtc tcaaatactt ttgctttttt cggatacaat ttaactaact
     4921 gatccttgat atttgaaact tccttaattg ccattttggg tgaaacccac gtatgaggat
     4981 ctagttcatg atggtgatct tcttctccgt gatcatgatc ttcttcttca ctacctggca
     5041 ataaaatcat gtcttttgtt ccttctacta cattaggccc agctttcttc caactttctt
     5101 tagcttttgg tacccatgat tccatgtttt cgttatgata tacaaaaaca tcagcatcat
     5161 gagcttttgc catatcctta gctgagggct catagtcatg aggttctgat cccgctggaa
     5221 ccattaaatc cacgttcgct tcatcgccga cgatgttttt ggtgaaatca tacattggat
     5281 agaatgttgt tacaatttct aattttccat ctttatcatt cgatactttt gcatcattag
     5341 tgctggagca agctcccaat aatcctagag ctgctatccc aataagtcct acactgattt
     5401 tcttcattct ttttccttcc ttaattatgc taatttcttc attttatttc gtttatgttc
     5461 tttgataatt tttaatgccc tttttctagt tttaatattc gggcttttaa gtcgtcgata
     5521 tgcactagat atatccgttt tctccattag aaattacccc caatcactac ttatcattat
     5581 ttgctttccg ccagtaattt atcaaaactc tgtttgatcc tttcttcatc caacattttt
     5641 ccgattagaa taatagtggt ttggtctttt tgtcgagtgg tgtattgaaa attaacctgt
     5701 tcgtttacct cttgcatggc aactataaaa tcctgctctt gtaaagcaat caatccttta
     5761 aaacgataca ggctcccttg gttggttaaa atcagccagt caatccattg gagtaaaaaa
     5821 tcttttttta tgtcttgatc tgaagttaag agaattgatt taaactcatg tacttgatgg
     5881 tgttgatgat agtatttgcc attatagtga ttttctgatg aatcactttc aaaaactaag
     5941 ggatgattaa attttttcaa attataaaaa tccttttttt gaatcgtttc actatgtgtg
     6001 aacattcgaa tatcggataa agggttaata cttttgattt ctctctgtaa tttttttagt
     6061 gcagataaat tcaagttttg ttttctagac acaaaaatgc gatctgccat gacaagttgc
     6121 ttaagggctt gtggttctct ttcaataagt tcaaaactgt cactatcaat aacagtcaat
     6181 aaactatcga tatatactct tcctcttagt tgaggcgctg ttaaaagtgt ttgaatgatt
     6241 ggttggggat ctgctatacc tgtagtttca atgatgattt gtgaaattgg ttgttgatgt
     6301 tcttctattg tttccagtaa agaaatcaat attgctaata aatcactgcg aagactgcaa
     6361 cacatacatc cattatttag ttgtaacacc cgttcctcgc tatgaactaa taatttgtga
     6421 tcaatcccaa cttcaccaaa ctcattttca attataataa tttcttccct ggggtagggt
     6481 gagacttgca aggcttgatt gattagcgtt gtttttccag cccctaaaaa cccagaaacg
     6541 attgtaactg gtatgcccat tttgtcacct ttttaccact tagctatttc acttctttaa
     6601 aaattgcccg tctgcgcaat tttggcgaat acttttttaa ttctaaccgt tcagggttat
     6661 tccgtttatt tttgctggtg agatacagac gttctccagt ttcgacacat tccaaaataa
     6721 tattttgacg cataaatctt cctccttcaa tacttatcga tcttcaagat atgcttgaat
     6781 ttgttgtgca tcttcaaaag ttaactcgcc agattccaat tgtgaaacta aattttcttt
     6841 catatcaaca ggtatctccc aataaccatc gatacattga aataactcct cgtcatactc
     6901 ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat aagccttggt cctcatagta
     6961 atctttgact aatccatttt caattgcctg ttgggtcggt aaacccgttt cagtaataat
     7021 tcctctctct accatcgaac gataaaaagt gcatttatct agcgattgct gggtcgtttc
     7081 aagataatac tttaatcctt cttcaactaa aggtgggtaa ataatttcca ttttgattcc
     7141 ctcctaaaag ttatatatta aactattttg aagctccttt aattactttc tttccattat
     7201 agtacccttt taaagataca tgatggctac gtctataatc accattcgct tcgtcaaatg
     7261 aaatttctgg ttttactaca gtttgatgag ttcttcttaa ccgtttcttc gccttagatg
     7321 tttttcttgc tggtactgcc atgatatttc ctccttttac caacttgctt tttttactcc
     7381 aggaatcaac ccttgatggg caagttctct aaacttaata cgggacatac cgaactttcg
     7441 catataccct cttggacgac catccgtctg atcacgaagc ctcagacgat tcggatttga
     7501 atctcttggc aatttagcga gagcggtgcg atctcctgcg gcttttaatt cctgacgttt
     7561 atctgcatac ttttcaatca tcgccatttg tttctttgct ttagcaattt tcgacttttt
     7621 tgccatatac ctttatactc cttttatttt gatttagaca cctcaaatcg taatcgttac
     7681 gttttgacac tgatacttta ccaacaagag ttgctcttgt caacaataaa taacatgaaa
     7741 taaaactcat atgaatagat aactatttga aaacgaattt atcggtacat agagataatc
     7801 aaatcattta aaagaatatg cagtttaggc tctgaaaaat aagaattcac ttttattagt
     7861 ccaatcaaac acaaataccg ttttaagtag cttttcctct acttaaaacg gcaatatgaa
     7921 agaaactata actttttatt caatctttta tgtttaactg gtttatcgcc aattttttaa
     7981 aactttgatt attccttata ttataagaat agacattaag tgaactaatt caatcatact
     8041 gattctttcg tgtattcaaa attagatgct gtttaataaa attattcttt ttgaaaaaga
     8101 attataacta tatcccaaaa attgtaggtt aatttaaagc ctatttcatc atcgtgattg
     8161 agcttctttt tcatagacga atttctcaga tagtttagtt ttctttatga gatttttgta
     8221 tatttctgac cttcgataca actcattatg agtaccttga tcaactagtt ttccttgatc
     8281 catcactaca attttattta ccttttcaat agacttcaag cgatgcgata taattagtac
     8341 tgtcttatct tgtattaaac gatttaaact ttcctgaata cgtttttcat tttccgcatc
     8401 taaactggca gtaatttcat ctaaaattaa aattggcgca tttttcaaaa aagcccgtgc
     8461 tatggatagg cgttgtcgct ctcctccaga aagtgaactt ccgttttctc cgaccaatgt
     8521 atgataaccg tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac gagctgcttt
     8581 tttcacttcc tcatctgacg cttgactatt tccaatacgt atattttcca aaatacttgt
     8641 attaaacaaa gttacctctt gaaaaacaat agcgattttg ctaaacaaag aagctggaga
     8701 tacacgttta atatcatacc catcaattaa aatacagcct tcatcataat catacagtct
     8761 agacactaat ttcaagatac tagttttccc ggaaccacta gcacctacta acgcagtcac
     8821 ttctccttgt ttagccgtaa aagagatatg atctaaaatt ggggtattgt tatcatatga
     8881 gaaagagaca tctcgcaact caatgtcaaa agatttcaga ggactatctg atccctcttg
     8941 gatactggtt tccttcatag ctctaattcg ctgtatttta ggagctaaat aaaatatttc
     9001 aagcactgct tctttcatag aatcgaagga atcctttatt tttattgcgg ctagtaagta
     9061 accaacgacg tagagtatag tcacttcacc cgttaacagt aagtgaacac ctactagaat
     9121 gactactgct aaactaacat atgagaaaat agaggataag gccatgactg aaaaagtact
     9181 taattctacc tttaaatgaa tcctttcact ctcttccatt tttttgaaaa gtctgtcttg
     9241 aactttttta gatagattaa agctattaat ctcttgctgc aattctatag tttcttgaaa
     9301 ttcttccgag ttttctctca aagtatcgta atatttagta ttggctttcg tttgggattt
     9361 ctttgataac aagattaaga caaaactaaa taacgttggc aaaataacag ctaatcccat
     9421 tttgacatta ccaaccaaaa gcatcactga aataaaaggg aaaaacagag ccataccgcc
     9481 ggctttaggt attgcatgac tcatcgcatg ctcaatacct tcaacgtcag acatgatagt
     9541 ttgagataaa tctgataaat tatgttttga aaaataggat aatggtaaat ttgacaaatc
     9601 gtctgcaatt tgcactctta aatgcgcact ttctttataa gtactattat ataagttttc
     9661 atactcccta tctaacaacc aatacatgaa aagcaaagta acaaacgata cggcaaaata
     9721 aagccaacgg ggctttgtat tttctaaaac taattcgtct atcagcatca ttataatgaa
     9781 tgctggtacc atattaatgc agtatacaaa aaaactagaa atagaagctt tagttaaagc
     9841 ttctgcgccc tgatcactta aagcaaattt gtttttataa aaacttttca ttgtcaagcc
     9901 tccaatcatt tgcattctca taattagtga ccaattgttt atacacttga tgtttagcca
     9961 ttaattcatt atgatttcca cgttctacta ttttcccttc tgacaagaca agaatttcat
    10021 ctacagattt aatgctggat aatcgatgag ctatcataat aactgttttt tctttcatca
    10081 gatgttgaaa agccttttgt agttcatact cattatctgg atcaatagat gcactggctt
    10141 catccataat aacaatcttt gcatctttta aaattgctct ggcaatagct atacgttgtt
    10201 tttctcctcc tgacagataa actcctttac taccaattaa ggtatgctct ctttcgggaa
    10261 atttttctaa aatcgattcg caaccggcta atcttagcgc gtcaaatact ttacttgctg
    10321 gcgcttgagg atttgctata gaaacgttat cgtaaatact ctgattgaat agtttcgtat
    10381 cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga tttaaaggct
    10441 taccaccaat tttaatctgt cccttatcca cattgtagaa acctgaaatt agtttagcta
    10501 tcgtactctt tccgcttcct gattttccta ctaatgcata aattcttttt tgttctaaaa
    10561 ctaagttgaa attttctaaa acacactctt ctttgtatga aaagctgaca ttttcaaatg
    10621 caatgtcaca atttttaatc tgtgtctcgt tcccatattt tagttggtct tgcttcattt
    10681 ctaaataaag ggagtctagt gtttcgactg catatcttgc ttcaaacaag tgcatactta
    10741 catacattat tctcatgaaa gaaacaaaaa taattccact taaaaaaaac aacatcaaca
    10801 agtcaacagc taatatcttg ggagtggtaa gagaagttat aaaaaaggtt ataggaacta
    10861 tcaaaatgca aactatgcca aaaaatagcc attgaaaaag aacaaaaggt gttttcccac
    10921 tcaaagaata cttatatgca aacttagcat aatcgttaat tgctttattc aaggctttaa
    10981 atgatgttac tttagcacca aatatcttta taacttgaat tccgcggata tattctaccg
    11041 tttcagagct taacacatct aacgattctt gatatttttg aataaaattt gtattctctg
    11101 tcattttctt taacaagaat acaccaatta ctaacatggc gattagaacc aatccaattt
    11161 taaagttaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg gcttgagcac
    11221 tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca tcaattgtct
    11281 ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct gttaaaccgc
    11341 taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga gaaaataagc
    11401 cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc aacagtttga accgcataac
    11461 tcttagctaa atattcatct cctacaataa ttagagactg aagaaatttg taaatactgt
    11521 aatatccata gactgttaat acgacagaaa aatcagaaaa taaagtggct aaccatccgt
    11581 agtatttcat tttagggaca taactaaata attttttgta tactttcatt ttttcctccc
    11641 ccgcattcaa tttcttctct tattcgttta caactgttaa tgctttccgt aatagcgtaa
    11701 attagagatg tacaaacaga taaaaggtaa actacgttct attttttgta tttttttaag
    11761 acttttcttc caatcaatac accaatcata gataaacttg ctacaactaa cactgaaatg
    11821 attccccata tacctaccgc atagtggatc attgcatcca ttgttgcagc agattgtcct
    11881 ttatctatcc aatcttgacg aaaacgatct gtaaagaacc atataggaat ccactgtccg
    11941 gcagcactgc ctaattgcat tagagcccaa gacgcaactt gaggaattgc tcgatcatat
    12001 ccaaatacag atagaataat atcagctaag atcgccatac taatcgaact gataaaccaa
    12061 ggtaagtacg ccccgcccat taacgtaaaa aataacaata aaacaattgt gtaaatagta
    12121 aaaattcctt ttctagggta gttatagcta atataaacaa atatgattcc agataataat
    12181 ccaaaaatcc cgggggatat gctgtgacca aacgccccac caatactact aaccattatt
    12241 gaaaaaaagt aaattactag ggaaataact gtagctaaac caataaaaat ataatctttt
    12301 gttttcatat atattctcct caattctatt tcgttatgtc attaaaaatc tgttctatca
    12361 aagttgaatc ttctactcta agttttatat ctttttttat tccaccattt tttaaataaa
    12421 taattctatt gcatgtttta taaagaaatt ccggatcatg agtagctata actactgcac
    12481 ccttttcttt taagtcttta ataaggtcag caatcctttc catattaata taatccaacc
    12541 cacttgtagg ttcatcaaat agaaataaat tagtttttga caatgaagct atagaaacca
    12601 ataatctttg tttttctcca ccggataaat caaacggtaa ttgatctttc attttataaa
    12661 gttccatttt ttttaaaatt ttttcttgct gatctttact aactatttca ttccccaggg
    12721 ctaactcgct cctcactgat tctgtgaaaa attggtaatc catttcttgc attactaaaa
    12781 acggcgatga caaatttttt ttattttcga ttatctttcc tttggtaggg cgaataatct
    12841 tcatgagtat tcttagtaag gttgttttcc ccacaccgtt tttccctacc aaaccaataa
    12901 catctccaac tttgacatca aaatttatgt cttttagaat attttcataa gtaatttctt
    12961 caacttttaa tacatctgca cttacctttt cgatataggg aacttcaatc gaactattaa
    13021 aaatgtcaaa tttccttaat gcgttattgt tttcagtttt taacaactga atagttgtag
    13081 tttttacagt tctattttta acaaatagaa cttgatcaat taattctttc aaatagtaaa
    13141 aacgatgttc agctataaca atagtaaatc ctttattttt taaatcacaa agtaagtcag
    13201 caagtttctt agtcgctttg aagtccaaat ttgaagacgg ttcatccaat ataattaatt
    13261 tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca cttgatagcg
    13321 aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat tcattgattc
    13381 ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact aactcactcg
    13441 ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca atattttttg
    13501 atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt tccccaacca
    13561 attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt gtggatttcc
    13621 cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa aaattaaggt
    13681 caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta aattcaaaca
    13741 attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa caggatgata
    13801 tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg agattctatc
    13861 ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg tataagaacg
    13921 ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg aatgtttctc
    13981 atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa gaatctgatc
    14041 actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa agaggataat
    14101 aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac cggcaacatt
    14161 cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa aaaactctat cttgatagca
    14221 tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata aatacatgtc
    14281 tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc cacttgaaat
    14341 atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt
    14401 aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact gttatcttaa
    14461 gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa gcataactga
    14521 gaacaattct caatgcaagc tttttttaat taactcagta attcaatcca cccaggaaca
    14581 gaaaattgat acaatcttct ggttaatagc atagtctttt cttttgaata gctatgcttc
    14641 aatatatcat agaatgtagc gtagaatgag taaatgtaga agtggatttc ttcttcatta
    14701 aagactaaat tttgattgat cattccgata gtctttaact tatctagaca acagactgtc
    14761 tttttcgtca caaatgatgt aattttttca acaatatctt gatatttggt tccagctgat
    14821 tggaataaaa tcaaatcgaa tatacgttta ttctcaaaaa tataattaat aaatggatct
    14881 aattcattga agttctctct ccaacttttc tctatatcta tattttctaa gtttaagagg
    14941 tatttggtaa actcttcatt atacatatta ataagcgcat taatatgcgg ctgaataatt
    15001 tcttcaaata acgcttcctt agaagtaaaa tgtttataaa aagcacccgt agttacgcct
    15061 gcggtcaggc acagttgcct tatcttagtt tgctgaaatc cttctttttc aaaatggctt
    15121 agtccacttt ctattaactt ttggtgagtt ataatataat ctctggtttc tatgggtact
    15181 tcctcctaat aagatgaatt aactttatta tcttacacag gacttgaagt ttcaatcgtt
    15241 aacgtttatt actgaactca ttccttagag ctaaactaat gagaaagagc agaataactg
    15301 atacaaaaag caatcctaca agaattaata gactcatcta aatcattcac cttttctaaa
    15361 ataatccgag aaagatttaa aaaaattata tccagctatt aggataagtc ctatcataat
    15421 cactccaata gccattttct tatcctccct aaaaataaag tcttttactt aaaacctttt
    15481 ttgtaatcgt caataaattt tttcccttta tcagttaatt gaaatcctat atctaaatag
    15541 tcctcatatc taagtttagc cagcatctca cttacagatg ctggactaac acttagaact
    15601 tttgctaaat cattattttt taatttcttt tttctttttg ataattcctg aattgctagt
    15661 aaataaatta gtatagtagt cgttttaatt tgtaccatac ctaccctcca aattatttcg
    15721 aaaggccttc agcaatttta tccaggttcc atttcatcat cgcatagtaa ctatcaccat
    15781 cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga atagatagga atattggtat
    15841 cttttgatac tgttttcatt ggtctatcat ccacactgct ttctacgaat aaggagggaa
    15901 cttttgttgt gcgtaatttt tcaactaagt gttttatttg atctggtgtt ccttcttctt
    15961 cagtgttgat ttcccaaatg taagcagaag gcacattata cgctttcgag aaatatttaa
    16021 agcatccttc acttgtaaca atcatttttt tatcattcgg aattgaagca aatttagatt
    16081 tagcttcttt gtctagagaa tccaattttt caatatactt atctagattt tctttataga
    16141 attttttatt atcaggatct ttttccgcta actgtttttc aatattttta gcgtaaataa
    16201 taccgttttc taaatttaac caagcatgag gatcttcctt ccctttctca ctctgaccct
    16261 ctaagtaaat aacatctatg ccatcacttg ctgcaaaata gtctttgttt tcctctttgt
    16321 tcgcattttt aactaatttt gtaaaccaag catttcctcc agtttccaag ttaacaccgt
    16381 tataaaaaat caaatctgct tttgaagttt tttgaacatc ttcaggcaaa ggttcatatt
    16441 cgtggggatc tttcccaata ggtacgatac tgtgtaaatc aattttatct tttgctatat
    16501 tttcggtaat atctgctaaa atcgaattag taactactac ttttaatttt tcgttcgaag
    16561 ccactttatc tttgtcttta ctatttattg aacatgcccc gaatataaaa agagcaatca
    16621 aaatccccag acttcccagt actgctttcc attttctcat cataacgatc tctccttctt
    16681 tcttaaaaat ttttgttttg gtgagattag aaaactaatt acaaataaga atgccgctgt
    16741 taaaacaata cttgatcctg ctgctatgtt aaaactataa ccaataaaca atccaagcaa
    16801 tgatgaactt gctccgactg ttgcagaaat aaatatcata gttttcaagc tatttgcata
    16861 taaatatgct gtagctgctg gtgtaattaa cattgcaaca attaatatag ttcccacact
    16921 ttgcatagct gttactgcta ctaaagttaa tagaaccatc aatagataat gataaaaact
    16981 gacattcatc cctattgctt ttgccattaa tggatcaaaa gaagtgatta ataattcttt
    17041 aaaaaataga actactataa ccaacactaa aaccgaaacg ccaattgtaa tccacttatc
    17101 aatatcttgt acagctaaaa tatttccaaa taaaatatga aataaatccg ttgagctatt
    17161 tgcaactcct ataaggataa ctcctagtgc taaaaaagaa ctgaaagtaa taccaattgc
    17221 tgtatctcct tttattacac tattgctttt tataaaagta ataatgatag aagctaatag
    17281 accaaataca atcgcaccaa taaaaaaatt aatacctaaa atataagaaa ttgctactcc
    17341 tggcaaaaca gcatgagaaa tagcatcacc cataagagac attcctctta aaataataaa
    17401 acaacctact gttccagaaa caatccctat aactacagat gtaattagag cattctgtaa
    17461 aaagtgaaag tcatacagac cattaataaa attttgaagc atattattcc ccctctccaa
    17521 taaatatagt atctccatag gcttttttta aattttcttc attaaataca gattctacac
    17581 ttccgtgagc aattagattt cgattcaaaa gaataatatt atcaaaatac ttctttacct
    17641 tgcttaaatc atgatgcaca attagcactg tttttccctg atgtttcaat ttttttaacg
    17701 tatccataat tattcgttca ctaactaagt ctatccctac aaatggttca tctaaaaata
    17761 tgaaatccgc attttgagca agacatcttg ctaaaagtac acgttgaaat tgtcctccag
    17821 ataattctcc tatttgatga ttagaatatt ttcccatatc gaccttttcc aaagcctttg
    17881 aaactctttg ccattctgca tttttaattc tttgaaaaac tttcattccg gcatatgttc
    17941 ccatagatac acattctttt acttttattg gaaaagtaaa atcaatatca gctttttgct
    18001 ctacatatgc aatcttcacc agttttttat tgatatcttc attattaatg gagacctctc
    18061 cctgatgagg gataatattt aacattgctt taagcaatgt ggattttcca gcaccatttg
    18121 gaccaataat acctgttaaa gcaccttctt gtatttttaa agaaatctcg ttcaatgcta
    18181 aaaaatcgtt ataacaaaca ttaagctttt tcacttctaa cataactttc tccttctaaa
    18241 tgataatgat tatcacgatc atatggtata caaaaattat attttagtca accctaaatt
    18301 ttatagttta gatttttttc tgttgctgaa gattagaatg acaaaactat ttgaaactac
    18361 gcgtatagta agtagctttc aaaatatata aaaaaggagc gttctcaaat gagaacatgc
    18421 tcctaaaact actttattgg cgtatggaaa aattatactt gaagtggata gcgggttgaa
    18481 taagttgata agcatcaaaa catttataaa aattattcaa gactatgtct gttccatata
    18541 caaatatgaa aatcagtaaa ctccatttaa cttttttcgt agtgtgtctt tttatacatg
    18601 tttatcgtta ttaatagtga ataggaaaaa aactattttt gatcattgac aattaaatac
    18661 aatttttgaa taacgatccc tgctattcga gctagaaact caagtacgcc atgacctgat
    18721 tagcgacaaa tacttagaga tttctttttg ttctgggaaa acaaaatgcc atgacaatag
    18781 cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg aaattcctat
    18841 gtgcttaccc aaagcaattc aaaaatttca taactactat tttaggagat gattttatag
    18901 aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt tctaggaatc gataataata
    18961 ctctagataa gtggattcgt atatataatt tcccagtaat aagagttggt ggtgtagttc
    19021 gctttgacgt agaagatatt aaagacttta tgaatacatt taaaataact tgaaattttc
    19081 ttctcgccgc atatactaaa attagtgaaa tatactgcct tacttttttt aaggagtgag
    19141 gattttgcct acaaagcttt caaatgggaa atataaaact aatctgagat atccaaaaag
    19201 gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa aaaacatttc caaataggca
    19261 attggcgatt aaagccgaaa atgatatgaa gaagaaaata gaaaaagttt tgagagagga
    19321 gaatgcaaac tcattagaat taaaaggaaa aataacgttt aaaaaatttt acgaatctaa
    19381 atggcttcct agatatgaat taggtcaaac aattagatca aacaggcctc ctagtgatat
    19441 aaccattagt aatacaaaag atatatttcg gttgcatatt ttacctatgt ttggtgaata
    19501 cgcaatgaat tatctaaata tcaatactga gataatatca gatgaattaa ccaaaaaatc
    19561 gaaagaatat gcaaatataa aaatcataaa aggatatgtt cgttccatgt tcgatattgc
    19621 agaaatacta aattatatcg aatttaatcg aactactaaa attattcaaa gtatcactgc
    19681 acctaaaaaa aatgctttag aagaaaaaag aatacaagaa ggtaagcaag cactatcatc
    19741 aaaagaactt actaattgga tagaagcagt aaatgatgat tttaataatc atttacttac
    19801 ttttcatgat tacacacttt ttatgattac cctgtatctt ggtgatagaa aaagtgagac
    19861 ttacgctttg caatggaaat atattgattt tgaaaaacaa acggtccgtc ttaaacatac
    19921 tttagataaa tatcaaagaa aaaagtttac aaaaggtcga aaagataccg tcattcaagt
    19981 accagaagtt gtaatgacat tgctaagtga atggaaatcg gtacaagctg atcaattgtt
    20041 gaaattaaaa attaagcaaa cgttagatca gtatctcttt acttatacta aaccatctgg
    20101 agaagtaaat tgtcctgttc atgccgatta cctaaactat cgaatcaatt caattaaaag
    20161 aaggcatcct gacttagttc atttatctcc acacaaactc cgtcatactt atgcaactat
    20221 agctagacaa ggtggagcag atatgaatca aatttcaaat gctttaacac actcagatat
    20281 ttctacaaca aagatatatg tgaacacacc agatatagtt gataaggctg tttttgaagc
    20341 ttttcagaga ggattgaata agtgtgattg atatgtgttc ttaaatgtgt tcttaaaaac
    20401 aaaagaagca ctttccaaat aattgaaaag tgccagaaac gttgatatat aaaggatatt
    20461 aacgttttga gaattgtgaa gctttacgtg ctttcttaag acctggtttt ttacgttcga
    20521 ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat
    20581 caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc
    20641 ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag
    20701 gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat
    20761 ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac
    20821 ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg
    20881 tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt
    20941 tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag
    21001 tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc
    21061 ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt
    21121 tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag
    21181 ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa
    21241 catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg
    21301 ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt
    21361 ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat
    21421 gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt
    21481 tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct
    21541 tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc
    21601 acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg
    21661 aacggcaggc t
//
LOCUS       DACECS010000045        21214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.045, whole genome shotgun sequence.
ACCESSION   DACECS010000045 DACECS010000000
VERSION     DACECS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.045"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..256
                     /locus_tag="IWO31_001792"
     CDS             29..256
                     /locus_tag="IWO31_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302203.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            321..893
                     /locus_tag="IWO31_001793"
     CDS             321..893
                     /locus_tag="IWO31_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302204.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            894..1205
                     /locus_tag="IWO31_001794"
     CDS             894..1205
                     /locus_tag="IWO31_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0302205.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1196..1456
                     /locus_tag="IWO31_001795"
     CDS             1196..1456
                     /locus_tag="IWO31_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302206.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            1617..1976
                     /locus_tag="IWO31_001796"
     CDS             1617..1976
                     /locus_tag="IWO31_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302207.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            1973..3616
                     /locus_tag="IWO31_001797"
     CDS             1973..3616
                     /locus_tag="IWO31_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0302208.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            3651..4733
                     /locus_tag="IWO31_001798"
     CDS             3651..4733
                     /locus_tag="IWO31_001798"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302209.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            4777..5949
                     /locus_tag="IWO31_001799"
     CDS             4777..5949
                     /locus_tag="IWO31_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0302210.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            5936..6640
                     /locus_tag="IWO31_001800"
     CDS             5936..6640
                     /locus_tag="IWO31_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0302211.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            6645..7805
                     /locus_tag="IWO31_001801"
     CDS             6645..7805
                     /locus_tag="IWO31_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0302212.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            7881..8159
                     /locus_tag="IWO31_001802"
     CDS             7881..8159
                     /locus_tag="IWO31_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0302213.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8140..8511
                     /locus_tag="IWO31_001803"
     CDS             8140..8511
                     /locus_tag="IWO31_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0302214.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8527..8931
                     /locus_tag="IWO31_001804"
     CDS             8527..8931
                     /locus_tag="IWO31_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302215.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            8928..9338
                     /locus_tag="IWO31_001805"
     CDS             8928..9338
                     /locus_tag="IWO31_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302216.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9399..9986
                     /locus_tag="IWO31_001806"
     CDS             9399..9986
                     /locus_tag="IWO31_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0302217.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10059..10526
                     /locus_tag="IWO31_001807"
     CDS             10059..10526
                     /locus_tag="IWO31_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAQ0302218.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10572..10880
                     /locus_tag="IWO31_001808"
     CDS             10572..10880
                     /locus_tag="IWO31_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302219.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11075..17809
                     /locus_tag="IWO31_001809"
     CDS             11075..17809
                     /locus_tag="IWO31_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0302220.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            17813..18685
                     /locus_tag="IWO31_001810"
     CDS             17813..18685
                     /locus_tag="IWO31_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302221.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            18682..19309
                     /locus_tag="IWO31_001811"
                     /pseudo
     CDS             18682..19309
                     /locus_tag="IWO31_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19270..19731
                     /locus_tag="IWO31_001812"
     CDS             19270..19731
                     /locus_tag="IWO31_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302222.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            19735..20559
                     /locus_tag="IWO31_001813"
     CDS             19735..20559
                     /locus_tag="IWO31_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302223.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20577..21122
                     /locus_tag="IWO31_001814"
                     /pseudo
     CDS             20577..21122
                     /locus_tag="IWO31_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7625 a         3171 c         4488 g         5930 t
ORIGIN      
        1 tttttcttta cataaaggag gctgcataat gagaaactac tggtatgtat cactaactaa
       61 tgaatatcct cgaaccattg gtgattgttc agtgcgtgtt gtgcgttctg tacaaatcaa
      121 agggaagtac tctattgtcg aaatgctaag agaagctaca ccaaacgaag tggataaatg
      181 caagctgata tattgcggtc atggctattg gaaagacgag tatatccaat acaacattga
      241 gaggtacatc aaatgaatga taactatgat tacatcaagt tgttagcaag taattattag
      301 atgtttttag gaggaaaact atgaaattaa aagattatat cagagaaggg tataacgttg
      361 taactgcacc caatctagct tacaaaattc aagaagatta tcccaatgct ttagtgttta
      421 ctgatagagc tttagatgcc attcctatag ggaagttatt agtagatcgt ttttatagca
      481 ataatccagc tgttcttagt gctaagccac ttcaaaacgc ttatgctatt gaacgcttta
      541 cttgcgagtt tactggatat gagacggtag taccaatgac aggtgagaca ggaagaaaag
      601 taattcaaca gatcaaaaag aggattgctg aattgacgtt agatgatgga agtaatacga
      661 accttctaga aatcaaacta agagatactg actcagtacc agaagtttat tataaaggtc
      721 aacgaatgga tgagatgccg ataggtcttg tcgatattac ttatcattgg gatacggaag
      781 aatatgaatg ccaagtacca aaaggagcta acgacatcac tattgaatat tatgattcac
      841 ataataacaa gtacttagat agaaaaatca ttgggcacaa gagagatatg taaatgattg
      901 atgtttcaag taaacaagca agagctaggt tctatggatc aactgagtgg aggaaactta
      961 gaagtcaatg cttagagcga gatcattatg agtgccaatg gtgtaaagca gagggtagac
     1021 taacaactca acatgattca attcttgaag tggatcatat aaaggagctg gagtatcatc
     1081 cggatttagc tctagatata aacaacctaa gaaccttatg caaagattgc cataacaaaa
     1141 ggcacaaccg tatgaaatat cgtggccaac aaaagaaaag aaagtgggat gatgaatggt
     1201 ggtaagaaaa acagcaaaca gagatgatgt caaacgagca gaagcaatcg ctatggttga
     1261 taaggctatt gataagttgg ttctatttaa gagtcagctc attgaaggaa caacaactgt
     1321 agaggatata tctatcaaca atagactaat aggtaacgct aatgaagtaa cagggacatt
     1381 taatattaat gtagacattg attatacctt gagtccattt gcacatattg tagaaagcaa
     1441 cgagcaagac ggctgataaa tcagtataaa gcgaagatat atagagtgaa atacccccgg
     1501 tcaaaagatt ttgagaagaa ataaaatttt tgggaaccgg tggatggggt caactctgca
     1561 aataaatgat gctattttca cacgaccccc tccccacgta gaaaggaagt gagttgatgg
     1621 ctgatctgca agagagaaat aggcttgttt ctgctgaaga aaggcgtcta aataaattgt
     1681 ttaaggatat tgataaggat aaaaagaagg tcgtttctgg tttgattacg caagcggcac
     1741 gcttaaaaat tcttcttgat gaaatgtgga ttgatatttc cgaaaaaggt gattatgagc
     1801 ttttctctca gtcagaaaat caaaaacctt acgagcggga acgaccagtt gctaaacagt
     1861 ataattcgag agaccaagca tatcaacgtg taattaagca acttactgac tatttgccag
     1921 aagaaaagcg cgaacctaca aaaaatgcag cgctagatgg tagtgatttg ctgtgacaac
     1981 attaaagcca ttctattttg atgaatacgt agatttatac gaaaaagggg taatcccttt
     2041 taataaagaa agaattcagt taattactta tttaaaaaac gaagtgctag ttcgggatga
     2101 tatctatttt gatgaagaaa tgattcaaaa gtttatcaag tacactgaaa aaaatttttt
     2161 tcctttagct aaatatcaaa aatttatcac acctttcatt ttctgctatc aaaaagagga
     2221 cgacgaggtg ttttttgatg aaattctaaa tagtattgcg cgaggcggcg ggaaaaatgg
     2281 gttcatgtca gcaagagact cattttttat tagccctctc tacggtgtca gaaattatga
     2341 tgtaacgatt acagcgaatt ctgagaagca gggaaaggta tcttttaaag aagtttatga
     2401 aatggtacaa atgcgcaaac tggaaaaaca attttatttg actaagatgg ctatagtcaa
     2461 caatgtaact aattcgattt ttagttatag gacaaacaat cctaagacta tggatagtgc
     2521 aagagatggc tgcttagaat ttgatgaaat ccatcagttt gaatcttcag acctagttga
     2581 tattcagcgt agtgggctag ggaaaataaa acatcctcga actttttata atggtacaaa
     2641 tggtcatgtt agagaaggtt tttatgacaa aatgttggaa cgagcacaaa agatttttag
     2701 tggagagaac aagaatgatc gcttgtttcc ctttatttgt aaactggata acattgatga
     2761 aatggataat cctaaaatgt ggtcaaaggc taatccaatg ttcgaagaag attcagcata
     2821 ttcaaaacgt ctgttttcta cagtgatgaa agaatataaa aaactagaag atgagccatc
     2881 aggtaggcgt gaatttgttg ttaaacgaat gaattttaca gaaggtgatg cagaatcaga
     2941 tattacgact catgaaaaat taatggctac taaacaagaa ttgccaaatt tgaatggtcg
     3001 ttcttgtgtg gctggatttg attatgcaag tattcgtgat tttgcaacag tgggtttgct
     3061 ttttaaaatt gatgataaat ttatctggca gcaacatagt tttgctagga aaaagtttct
     3121 agatacattc aaactgaaag cgcctattaa ggattgggag aaaaaaggtt tagtgacaat
     3181 cgttgatgag ccatcaattg atcctcgtca tttaattgat tggttaaatg aaaaaagaaa
     3241 aatccatcaa atagaaatcg tttgtgccga cggatttcgt atggatttgc tgaaacctct
     3301 tctagagaaa gaatcatata attttgagtt tatcagaaat ataagaggtg tgcagtcaaa
     3361 agttgctcct attattgaag atggttttgc aaatgaacgg tttatttttg gtgatgatcc
     3421 tatgatgcgt tggtatacaa ataattcgtt tatcaaagaa gacaaattag ggaataaaac
     3481 atttttgaaa aaagagccag taaggcgtaa aacagatggg tttcatgctt ttttggcagc
     3541 tttatataaa cgagaagaaa taactgatat ggattatgag gaagcatttg atatgttgga
     3601 tgagattgaa ttttaaggta tactccttat tgaaaggggt agttatagaa gtgaaaaaga
     3661 gatattttct atttgttaca atatttataa taatagcaat ttgtgttttg acagtatttt
     3721 taccacaaat tactgagtat ttaaattatc ggatatttac cacaaaagta gatgatagcg
     3781 ttactagttt aagtaggcta gaatttataa aaatatgtgc atctgtgttt ggtcctattt
     3841 tgactgtaat ggtatttgtg aatactttaa atatgcagaa aaaaacagag caaaagcaaa
     3901 atgagcttga agtaaagcaa gctaaggctg agaacgagag agcaaaaaaa gagctgttaa
     3961 cacagataga tcgtgagttc tattttttat taaatatgtt tattgaaata caaaaagatc
     4021 aaaatgccaa acaagctgtt attgctttac agaagttggc aatttttaac gaaaagggtt
     4081 ttaatcaaga ctatgcattt acttctaaag atttatcaga tagtgaaatt gggagtacaa
     4141 ttgaacaatc aggaccgctg aagggcacag ttgaatatat aaaatttgaa acatttgatg
     4201 taagcaagtc gatggggaat tatcaaacaa aaacaaaaga cgttgctaca agtttacacg
     4261 atcagaaagt agctatattg aatgaacaat atgaaaagat gcaaatatat cttggacggt
     4321 acttcaaaat gtttcaccga atagttaaaa cattaaatga gtattatgac gattataatg
     4381 attttgatgt gaagaggtat acaaaatata tagggacatt aagaacacaa atttctccag
     4441 ctgaattcca agtgatttta ttcaattctc tatatataaa acgaggtttt ggcttaggaa
     4501 ttcaacttat tggaagtggt ttttttggag atgattttga ttttgaaact aatcaacatt
     4561 ttgaaacaag tatcaatgaa cagtggtttc tatcactatc aactgttgac agtgataact
     4621 ctataaagcg acaaaaatta tctgaatata ttaaagaatt aggatccgta gaatataaaa
     4681 aaatagctaa ttttgaaagt ttatataaac tctttaatga aactaagctc tagaaatagg
     4741 gcttttttat ttttctttga aaggtggtga aaacatgtga gcttatttga tatctttaag
     4801 cagtcaataa gaaatgaaga gccttctgag tggataccgg attttgtata tggagaggaa
     4861 gtatctgctc gggcatacct caaaattatg gcaaaaaata ctgttttaga ttttgttgca
     4921 agaactatgt caacgttaga agttaagttt aaaaataaag atgggacatc agactgggat
     4981 tatattctaa atgttcgacc taattctgat atgtcagcag ctacattttg gcaaaaattt
     5041 ttttatcggc tcttagatga caacgaagtg ttagtaatat ttacagatga taatcaattg
     5101 ctgattgctg atgattttag tcgaacggag tatgccgtgt atgatgatgt ttttacaaat
     5161 gtgatggtaa aaaattttgt ctttgaacgt tcatggaaca tgtctgacgt catttatatg
     5221 gaatataata acgaagaatt agaaaggttt acacgaggtt tatttgaaga ttacgccgcg
     5281 ttgttcggac ggattctaga agttgcaatg cgtaacaacc aaattcgtgg gtctgtatcc
     5341 attgattcaa cgggaacggc gaatgatgaa aaaggaaaag atggtaagac tagatctcaa
     5401 agattacaag aatatattga taggatttgg aactctttta aaactaactc agttgcaatt
     5461 gttcccaaag tgaaaggctt tgagtatgag gaatacacca ataaacaagg cgtatcgaat
     5521 caatcgctag atgaactgaa taagatgaag tcgtctttaa ttgatgatgt tgccaacgcc
     5581 ataggagtac ctacggcgct tatttatggt gaaaaagctg aactggattc aaatattaaa
     5641 gcttttcgta aattgtgtat tgctccacta gttaaaaaac ttcaagacga attggtggca
     5701 aaaataatta gtcgtcaaga gtacaaaaac ggggaacgta tagaagttaa aaaagttctc
     5761 caaaaagatc tattagagta tgctactcaa attgataaaa taacatctgg tggtgcgttt
     5821 tatattgacg aagttcgtga tgaagttgat tatgatgagt tgccaaataa tgaaggaaaa
     5881 attcgtattc gtacgaaaaa ctatgaagaa actgtgaagg gaggtgagaa cgacaatgcc
     5941 gaaagctaaa aaagtaccat ttcagtttac taacgaaatt cagaatggta agcacattct
     6001 caccttgagt ggaaatgtac agaaaaaata ttggcgtgat gatgatgtca taaacgctaa
     6061 agatattcgt gactctctag atactgtaac cgatgatatt gtaatcaagt tgaacagccc
     6121 aggcggtgat gtgtttgaag ggattgagat ttacaattat ttaaaagatc acccatcaaa
     6181 tattactgta gaagttacag ggcttgccgc aagcgcagca acgtttattg tcgctggtgc
     6241 caatgatgtg attatgaatg ttgggacttc tttgatgatt catgaagctt ctacatttgc
     6301 ttgggggaat aaacaggaca tccaaaaaac gttaaacgca ttggaaacga tcgatgattc
     6361 tatcttagct atttattcag ataagactgg ccaatctgtt gataagttgc gtgaatggat
     6421 gaatgaagaa aaatggttta cagcagatga agctgtcaaa tatggatttg ccaattctgt
     6481 gaagcgtgaa gaaccacaaa atgaacctca aaatattgca gcgatgattc aagatgctgt
     6541 tgccgttgca atagcgaatt taaaggaacc tgttactaat aaaatcgaac aagaatcaaa
     6601 acaaaaatca ttaatcgcac gattgcgaaa aggagaataa agatatgtta aaaattacag
     6661 ataaaactgc agatgcaaaa aaggcattta atgctatttc ttcgaaagaa gatgcaacac
     6721 ccgaacaaat taatgctgct ctagaagaat atgtaaccgc cattgcagaa gatgccggca
     6781 aacaggtgcg tgctgaatat gaagaattga aaaatgtgac ggacaaccgt gtattagagg
     6841 ctcgtggaat tcctacacta acagcagaag aaacaaagtt ttataatgaa gccgtaaaat
     6901 ctggaggttt cgattctgac gtagtttggc cagaaacgat tttggaacgt gtttttgaaa
     6961 atcttcaaaa cgatcatcct attttaggcc tcattaactt tacaccaact gtaggtcgtg
     7021 tcaaagtaat tagagcacgt cgtaaaggtg ttgctgtttt tggaccattg cataaagacc
     7081 ttgaaggcca actagatgct gaatttggtg caactgaatt tgttcaactt gcgttgaccg
     7141 catttttctt aatttctaat gataccttag atttaggacc acgatggatt gaccgtttta
     7201 tcaatctttc tttaagtgaa gcagtacgtg atgtttgggc taaaaaaatt attacaggtt
     7261 ctggtaaaga tgagccaatt ggattgctta aagatctaga tggtgcggtc acaagtggtg
     7321 tatatccaga taaagctagc gcagggacat taacttttgc gaaagagaag attgttcttg
     7381 aacttgcagg ggtgatgaaa aaattatcta aatacacata caaagtagat ggtgacgatg
     7441 aaggtgaagt gaaataccgt tctgttgctg gacgagtgta tctaattgtt aatcctgttg
     7501 attactggga tatcgtagct agagttacat ttgcaaattt aaacaatgtg tatgggacta
     7561 cgatgccatt tatcgctgtt gatcatatca tcgaatctgt agacgtacca gaaagtaaat
     7621 gtattgcata cgttgataaa gagtatgaag ctactcaatc aagagctgaa aaagtctatg
     7681 tatataaaga aacttttgca atgaaacgcg caactttgta tgctgtcgac atgcttggta
     7741 atggctatcc aacaaataat gatgcagcac aagtatatga cttggacttt acagcggggg
     7801 agtaatgtcc cctgcaattg gtgagataac accgacgact gatggggcag tcattaatct
     7861 ttcgtaaatg gggttagagt atgaacactg aagaatttat aaaagaatat aaggcccgat
     7921 ttcgtatttt ccatacttcc gaagatgagg atatcgggaa acagttagag agcgggtttg
     7981 ccgatataaa atctctaatc ggcagttttg atccttccac ttatgagaaa ggaaaagaat
     8041 tagtttatga acgtactcgg tatctacgga acgaatcact agagtacttt tacgacaatt
     8101 ttcaaacaat gattatggat gcatcaattg atctggcagg tgctggatat gccaattaaa
     8161 tcaaattata agaagcctaa aattgtagct ggtgatctaa atacgccagt tacttttttt
     8221 gagtttcaac ctaataaggg tccagaacct ggtgatgaag aaaaaaagga actgtatttt
     8281 tgtacagcac tagtttacaa cccatcaatg aaagatcgtg acattcttgg cacaaaagaa
     8341 ggcgttacga taaaaatacg ggatccccat caatcttata ttcctactaa caaacataaa
     8401 gtaaggatat tagattatcg atatggagat aaagtttggg aaataatcga tgtctcaccg
     8461 gatttggaag aaaattcaat tgtaaagata gtattagaat taattactta aatttggtga
     8521 taaatgatga gtaacgtaaa gtttgaaggc gttcaagaaa caatccaagc tttagaaaaa
     8581 aagtatggag aaaagaaaac taagacatta acaaaaaaag caatcaatgt tggcgctgaa
     8641 aaagtagaaa aacagttgca aacggacatg accgttttta aagacaaagg ttatacgatc
     8701 gctgaggttg ttcgcaagaa cgctacgtat agagattaca aggcagaagc tgaaatcggt
     8761 tggaatggtc cacatcaacg atatagaatc attcatttga atgaatgggg atatacacga
     8821 aagggcaaac aaattagacc gagaggattt ggtgtaataa caaaatctct taagaattcc
     8881 gagctgttgt actttgatgc tgtaggagag gaggttaaaa agagtttatg aaagatatgc
     8941 ttaatatcat ttacgaggct ctttgtttga atgagtatat tcattcaatg acctacaacg
     9001 agaataccga gcaatatcgt attaaatttt atgagcaacc tgaaaccgcc gataaaacag
     9061 gtccaatgat cacaattcgt cctgtagata cacctaacga agcgtaccac ggaagtaaca
     9121 aagagctttc tgtggagcat atgatacaga ttgacgttga atcgaaatat agagcaacat
     9181 gcaagcagat tcaatatgag ataaaaaaag agatgaaaaa actaggcttt ggccaaattt
     9241 ccggtcaagg gttagatgaa tactttactg aaacaaaccg ctttgtagac gcgcgtcgtt
     9301 acgaaggaaa tacaaaaatt tacgataccg aatattagaa attaagacac gaaaataacg
     9361 tgtctttttt tattacccaa aattaggagg aaaattttat gactttagta ggattcaaaa
     9421 aaatgacaat cggggtcttt gacagcacag ggaagattcc agcagcaaat ttatatgtaa
     9481 ttgaaggtga acaaaacaaa ggggcgactg tatctgctga aatcagtggg ctatcaaaag
     9541 aaccatcaaa agtttacgga tcgaacatcg cttactatgt ctctcaaaaa ggaacaggcg
     9601 atgtttcagc tacttttgga ttgttagatt taccaacaga agtaaatgac aaaattcttg
     9661 gttacaaaac cgatacaaac aaaatcagct tcttgggaga agatactgag ccaccatatt
     9721 gtgcaatttt gatggaatcg gaggatttga acggcgatac agcaatgctt gcgatgttca
     9781 aaggaaaatt cagtcgtgaa tctattaatc ttaacactac gacaaatgaa gctttcgaac
     9841 cagaagcaga agaatatgta ttttcagcga ttgccaatga tgttgaaggt gatgctaaag
     9901 gccaaacagt cgcaaaatat gttggagacg aagaagcttc tattactggt ttgaaagaga
     9961 tggtatttcc tgcagcgggg gagtaactag ccccgttgtt ggagcaatca ctcccactat
    10021 taatggggca acagttgaat taagttagga ggataactat gccagataca tttaaaatct
    10081 ataaaaaaaa tggtactgaa tttacaaaag tcgcagaggg tgaaagtcca ctttctatta
    10141 ctggaattgc agcaaatact caagttgcta aaggagatta tcaagctacg cgtgtggtcg
    10201 gaggtcaaga atcagtaaaa gtagatattc ctgcattcac cactcttcca atttcagtta
    10261 ctggtgtgac gttggataaa acgacagctg aagttgaaca aggcggaacc ttaaaattaa
    10321 ctcctactgt aacaccagca aatgcaacag acaaaacagg aagctggagc agttcgaata
    10381 cagctgtagc tacggttact ggaggcaacg ttacagtaaa aacagacgct gaagttggcg
    10441 gaactacaga agtttcattt actactacgg atggcggtaa agtagcaaaa tgtactatta
    10501 cagttattga aaaagcagcg ggctagtctt cggactagct tttttatttg gaaaataatg
    10561 gaggaatcta aatggcaaaa gtaagaattg aattgaaaga agaatcagga aagaaagtct
    10621 atgaaaacat agacacaaca gggaaagatt atcgaaaagc tttagaaaca atcaaaaagc
    10681 taaatgaaga cggagtaatg atttggaatc gtttagacat ttatttggat tttgctgtag
    10741 gtatcttcag ggatagcaaa ttgacagctg aacaaattct tgatggctta ccatctgaga
    10801 aggtaatgag cacattggac gacatcctag gagaagtaat ggggattgaa agtaatccag
    10861 acccagacgc aaaaaagtaa cacccgaaga agcagaagaa atgtacttaa acctatgcag
    10921 agagttagtg aaagcgggat ggtcactatc tgacatagaa gataattcct ttgaaatgat
    10981 gatgaaaatt gcgtgcactt cgcctaagaa agagaaacaa aaagaagttg atcttaaaga
    11041 cttcttgaaa tccatgtaga gaggaggtaa acatatggca aatggaaaac ctttaggcaa
    11101 tatgattatc aaactagact tagacagttc agctttttca aaaggtctag ccggagctaa
    11161 aaacgctgtt aaccatcaaa tgaaagcaat gaaatcacag atgcaagtaa tgaactcttc
    11221 aggaaattct ctcggtgctt tacaagcaaa atacaatggt ttaggcggag ttctacaagc
    11281 aaatgaaaag caagtagaat tgctcactaa ggcttataag gatagttttg actctaacgg
    11341 aaacgctact gcttcgactg ctaaatacgc taatcaattg aatcaagcaa cagctagatc
    11401 agcaagttac gaatctcaga tgaaaacaac tgttggacag attgctcgga tgaaagtgga
    11461 aacggaaggg tttacgggga aactaaaggc taattctgat gtattgatta attctgggaa
    11521 aaaaattgaa gcctttggta aaggagtttc tgggatagga tctgccctaa ctgttggagt
    11581 aacagctcca atagtggcag gcgcaactgc tgttactagt gcagctattt catgggaaag
    11641 tgcttttgca ggtgtaaaaa agacaaatga tgaagttgta gactcgaatg gaaacgtagt
    11701 ttattcctat agcgacttag agaaaggtct gcgtaatctt gccacccaat tacctggtag
    11761 ccatcaagaa atagcaaatg tagccgaagc agccgggcaa ttaggtataa agactgaaaa
    11821 cgtagttagc ttcactaaaa cgatgataga catgggtgaa tcgactaata tgtcggctga
    11881 aactgcggca acttctttag cacgatttgc taatattacc caaatgtctc aaaaagactt
    11941 tgataaatta ggatcagtca ttgttgatct aggtaacaat tttgctacaa ccgaatcaga
    12001 aatcactgaa atgggattgc ggttagcggg agctggtaaa cagattggaa tgagccaagg
    12061 cgaaattcta ggatttgcta ctgctctatc ttctgttggt gtcgaagctg aagcgggtgg
    12121 gtctgccttt tcgaaggtca tgatccagat gcaactagct gtagaaaaag gatttggcgc
    12181 ttttgatcag ctgaaacaga tggcagaaga acaaggtgtt ccgtgggtta atttagtaaa
    12241 tgctgtgaga gacggtggaa aatcgttaaa agcagtatca gagcaaatgg gattcacttc
    12301 atctgattta aagaaaatgt ataaagaagc ggacaattcg aaaagttcgc ttgaaaattt
    12361 tgcgaatgtg gcaggtgtta caagtgaaca attccagaag ttgttcaaga gtgatccttc
    12421 agaagcaatc atcaagttca ttcaagggct gaaagattca gaaaaacatg gtacttcagc
    12481 aatcaaagtt ctggatgaca tggatataaa agaagttcga ctacgcgaca gtttattgag
    12541 agctgcaaat gctagtgaca tattcaaagg agcaattgaa aaaggaaacg gcgcgtggaa
    12601 acaaaataca gctttaacaa atgaagctaa taaaagatac gaaacaactg aatcaaagct
    12661 aaagatattg aaaaacgaag tgattgatac agcaatcgat cttggaggtc catttgtaga
    12721 cgcgttgcgt gatggtctaa atgtatcgaa accattgata aaaaacttag ggaatatggc
    12781 gaaagctttc tcagctatga gtgatgatca acaaagaaac attatcaaat gggttggatt
    12841 agcagcagca gctggtccag cgctgaagct tttaggaact ggtacttcag taataggtaa
    12901 agttacttca actactggaa gattgacgaa aagccttgtt gatttagcag cgaaagcagc
    12961 agaaaagaag gctatggatg cctttgcatc gtcggtaaca acagctggtg ctgcagctgc
    13021 aaagacagca ggagctggag gtattggcgg gttagtccct atgatgggaa aaacagctag
    13081 tgctgcaagt actgctgcgg gcgccagcgg aataggcgca atgacaacgt cactaggttt
    13141 gttaggtcct gctctacttg gtattgttgg cgttggtggc gcactggcag ttggctatgg
    13201 agcatggaaa ttatttggtg aagaagcttg gaattcctcg caaagagtaa agcaatgggg
    13261 aacagatgtc ggtgaggaga tagatagtac tcttgacgga gtacagaaga atattgaagg
    13321 tgcaaatggt caattcggtc ttctcaaaga gggttttact gttgatgatg caaataatat
    13381 ggcggctaat ttcgcaaccg ctggaacatc tttggaaaca tctttaacgg gtagaataaa
    13441 ggcattagac gatgcactaa aagggctacc tcaatcagtt caggatgcca tgaaagaagt
    13501 agctgaatca gaagaagaaa ccttagggca atctctttct aaaataaaag acaacaatga
    13561 agaaataaaa aaaatcagag aaagagccgc aaatcagaac agagacttga ctgtttcaga
    13621 actacaacag atacaggatc taatgaaagg aacttctcaa gcgtatgtgg atacgctcga
    13681 tgtttctgct aaagacagaa aaaagatact atcagccatg aatggtgatg ttgcacaagc
    13741 tacagaagaa gaagcgaaga cgtggttaca atctttagcg aaacaaaaag ctgcaactac
    13801 cgaaaattat agacaaatgc ttgaagaaaa aaaggcggct ttaaaggaaa agggagtaag
    13861 caaagaattc attgatgcat tagaagatga aatgaatgat tacatcaaaa ctacaaatca
    13921 aggattcgat agccaaatag ctgctataac aaataaatat ccacaattga ttaacgaagt
    13981 gagtttatca aatggtcaat tatttagcaa aacggcagct gctatggaca atactggtca
    14041 gtatacaaaa gtttttctcg aaagtaatga aaagatttta gcccaagcga agaaaacatc
    14101 ggatgaaata gcagataatg ccgaaaaaaa tgcagaacaa cttggtatgg tagctgatga
    14161 ggctacgcat gctggatcaa cttggaatgg cttagtttta gacgaaaaaa caggaaaagt
    14221 aaaaactaat gtacaagaag tagtgaatga agcaacgaag gatttcacta aatggaatga
    14281 attgaaacct gttgttcatg atgcgaaact aacaacgaat gcaaaagatg tcatttccat
    14341 agcggcagta caaaatggct attgggatga tatggaatgg aaagaaaaag aagcattatt
    14401 agaagatgag tgttctaaaa atgtatacaa ggctttagaa aatgctggta aatggaatga
    14461 gttagatatt ccttctaaag aagcaatcct aacttcaaat actcctgaag ttatgggtga
    14521 aactctcatg aatttgggat tatgggatac ttacaaactt caagtgaaag atttagattt
    14581 gaatgcgtat aaagtatacg atgctatcga tggatcagaa gaaaaattaa atagttgggc
    14641 tcaattagat aaccccacaa aagaattgtt attagataac tcagattttt ctacaaaact
    14701 ttttaattcc gaagaaatgt ttaaacgttg gaatcagtta tcatctaatg caaagtttct
    14761 ttcggctaat aatactaaat ttacagaggt tcttttatca tctgacagaa tgtggaatca
    14821 atgggtaaac cttcctgata atgagaaaag attacttgca gataacgaag atttagctac
    14881 cactgtttta tcttcagaag aaatatacgg aagatggact caattaccag ataatcaaaa
    14941 atatatgtta gctgacaata ctgacttagc aaacaaaata ttcgcttcag aagaatcatg
    15001 gaatgcatgg aaagcaattc cagacacagt taaaaacttg cttggtaata atattgattt
    15061 actggctaaa gtgaaagacg ggactgtaag tgtagaagaa tataacaaag ttatgccact
    15121 tcttaaaact ctatatggag ataacgttaa tttaaaagag gcaatcagtg gggctaaaag
    15181 ctctatagag aattacaaca aaaatatttt tcctagtgat aaaactgcag taggacacga
    15241 tgaggcttca caagctgcta aagatgcatt tgaagcgttc aacatcttcc aaacaaaaat
    15301 accagataaa attactaaaa ctgtatcagc tgactttata ggtcctatgc cgaaaaatgc
    15361 gaaaggaaca aacttccatc ctggcggagc agctatggta aacgatcaaa aagggcctac
    15421 ttatgaagag cttataacct tgccaagcgg agaaactttt atacctaaag gacgtaatgt
    15481 cgttttagac ttaccaagag gttcgaaagt actgaacgcc actaaaacta aacgtctagt
    15541 gcctaaatat gctgatggta taggaaacat aacgacggtt tcatctacgc taaacttaga
    15601 tgctttaatt ttagctatta acgagctaac gacggtattg agaactcaac tgatggcagg
    15661 aacgactgat gctaaagata ctaaactatc agatgaaaca aaagttgccg atccgattat
    15721 tccggacagt ctttctgaaa aatcagatca gtatctagga attggagctc aatggttaac
    15781 taatctaatg aatggttgga actcagcggt tcctcaatat atgaatagtg aaatggtttt
    15841 tattacaaac tatctcaatg cgctaaaact tcaaaacaat cctaattata cgcaaggagc
    15901 aacatggaat aagaacttac tgaatggatg gaatagttta actgggacct ttattgctac
    15961 aatcaattca ttttgtaatc aagcaatggt cacgcttaga aactacaaca ctcctatgta
    16021 caacaatggc cgaacgtggc agcaaaataa tcttaatggt tggaattcat tgtatggatc
    16081 attcatagcc cgtgtaaatc agcttggtaa cgattcgatt aacaaccttc gttctaaaag
    16141 tggtggtttt tacaatgcag ggacattctt gttgcaatct ttaataaacg gaatgaattc
    16201 gttaggtaat tctctttcta ctacaatgaa caatgtcgct aatacaatgg ttggtggtat
    16261 aggcaaagga gttaatggcg tcatatcggg agttaactat gtgcttaagg aagtcgaatc
    16321 aagtaagaat atcggaaact ggccaattcc acaatacgct aagggaactg atggacatcc
    16381 tggtggactt gctatgatta atgatcaaaa aggcctagtt cacgaagaat atgttcagat
    16441 gccagatgga cgtagtttta ttgccaaagg tagagatctt cttgttaatt tgcctaaagg
    16501 tgctcaggtc ttaaatgctt ctttaacaaa gaaattaaag gaacgtttaa atattccaca
    16561 ctacgaaaaa ggtattggca atttagatat tgttgatttg ttagatgatg aaaaaagaat
    16621 gttagaattc ttaactagca aagttgattt ttcaggcatt aatgaaccat ggcttgatat
    16681 gactaaatca ggaacgtcat taatgtccaa agctgcaaat acaatgcttc aatcaaaatt
    16741 aagtgaattc ttcactcatg gcaattttga tggtgcagta aatgccaatg gtgtttatca
    16801 atatttagtt gatgtggcac agaaagtaat gggtaagttc cccggactta cagtaacttc
    16861 tggatatcga gcaggagatg cctactatca cggaaaacgg caagctatcg acttggctta
    16921 tccaggtatt tctggagatc cgagatatac agcagcagca aattacgctt ttgaaaaatt
    16981 cccttcaaaa attgcgtatg tcattacgaa tggacgtgta cgtgaccgca tgggattatc
    17041 tggaacaagg tcaagcggac aatggacgaa ttggccagat ggcgatcact tcgatcatat
    17101 tcatttaaac ggttcgatgg ggtctggcga tatattcaat ggaggagttg gaggaagtgg
    17161 tgttgcacgc tggcgttctt atgtgtctaa agcattgaaa atgaatggat taccagctac
    17221 agcagcgtat ataaatgcct ggatgtccca aatccaaacg gaatctggtg gtaatgagaa
    17281 agcaatcggc ggcaatgatg ggctcgccga aggcaatgca acaggattac ttcaaacgaa
    17341 acccggaact tttgcagcta acgcttttcc tggtcacgga aatattatga acggatttga
    17401 taacatgtta gcagcaatca attatgctaa gaaacgttac ggagttgcag gtatgttgca
    17461 agttatcggg aaaggacatg gatatgccaa tggcggcttg attaccaaag acggactata
    17521 tcgagcagga gaaggaaata aaccagaaat ggttattcca ttgacaagaa aaacaagagc
    17581 tatcgaactt atgggtcaag cattagcctt cctttctgga agcaataaaa aagaaacgaa
    17641 aagtgagatt aattctgtag ataatagaca tatcctacaa gcgatagaaa aacagtctcg
    17701 tatgacaaat gatctgctat cattattact ctcgtttttt aaaagtaaca atcgatcaga
    17761 taaagaacta gctttagata ttcagaaaat tttaatcagg aggacatagt atttgaggac
    17821 agtaatatta agaaataaat tgaatgaaga aattgatttg tccacagaag attattttgc
    17881 tactgaaatg tcgaatctag gttttgaagt tacaaaagag catattggac aatgggggag
    17941 tttcagagaa ataggagaga gtattgaatt gtctgaattt caatcatcag ttatgatttc
    18001 tgttcacgga tttagagaac aacaattata tggttcatta gttgaatttc tggctaatgg
    18061 accttttgaa ctagaatttt cttttgattc agaaacaatg atcaggaaat gtagtttgaa
    18121 atcattatct aaaacagaaa ttgattccca aagctcattg ctaacagata ctttgaatct
    18181 atattttact tcaagctggt attctattaa gcgtgaaaaa ttaatacaga attcaacttc
    18241 tctcaataca cgtggaaaag tttactcatt tgttcgacca tatgtctaca ccaaaaatgt
    18301 atgggaaaag aaaggtgtat ttaagttcag taataattct gtatatttga caaatagtac
    18361 agagcgaatg tctccgttaa aaataaggat tattggagag tgctcaaatc catattggga
    18421 agttatccaa aactctaaag tagtagcttc agatggctat tttattaata tgaaagacac
    18481 acaaatatta gaagtgtcta gtttgctaga agaccaaacg gcaattttaa aagatgtagg
    18541 tggagttgaa tcttctgttt atcaacaaca agattttact aaatcaaact tcgtccaagc
    18601 gcctctagga gaattcagcg ttgtttttca tgttggacat gcaaatgtag agattgaact
    18661 ttatgaggag cgtgatctat tttgatttta gccattaccc tctttcaccg agatatgaat
    18721 ttatataacg aacgtttaat ttcgactgag ttcgaatttg gaacggatga aattggagaa
    18781 gaagtaagta attttattac agataaatat gtccatgtaa aatcaggaga ttttctacta
    18841 gcaaaattta ttcctagcgg gaagattgct tattttggag tgattagctc gcaagaagac
    18901 aaaaaaataa attgcaaagg attactcagt ttggccgata gcgagatacc gactgtaaaa
    18961 atatcaggtg ctaattatga agagcatatt cgacgactga ttgaatacta cttattgaac
    19021 gatccaacaa aacaactaaa aaatattttg gaggtaaaaa cagaaagcgt tacttctcat
    19081 tcttatcaag ctgttgacac aaataagaga cgattgaatg catatataat aaatgggttc
    19141 aaaaaatata atgttaaatg gtatgtcaaa gagattaaag gtaaaaaaat atatactggt
    19201 attcgagcag ttgatgattc aatttatatt aaagataatt catctgagtt taatgactgg
    19261 gatgtttttg tgcaatctcc tggaccaggg aacgagaaca aattattgat cgtagataaa
    19321 gcaatgaaag atatagaaaa tcccttggta ttgtctacgt ggtatttaga tgacgaaaat
    19381 aatcttacgc aagattcaac gaataagaac attgcattgc ctaccgttaa tttagtaaat
    19441 atatatgatc aaactcaaga agatagacct tcttatgaag aggtagctaa atcggagctt
    19501 aaaggcaacg cctattcaca tgaaattaaa gttaatgttg tgctaaatgc tagaaatatc
    19561 aatgtagaaa atatcgaaac aggaatgtta gcgactattg tttacaaaga gcagacttac
    19621 aaatctgttc taactgcctg gcgaatatcc agcagtaaaa agaatataga attgacattt
    19681 ggtaatgtcc gaagtcgttt tatggattat tttgaagata atgggggata aaaaatggtt
    19741 agcaatgtgg atggatatca atttgaaaac gtgaaagtaa gcgcagaaaa tgatgctaga
    19801 ctttatcacg ttttatataa tcggaaaaat caggttattg atggttacga tcagtctatg
    19861 aatttatctt caagcggatt aacagtaaaa gttgctgcag gagcagcgat tattcaaggt
    19921 cgtatggtcg ttgttcgaca agaagaaagt ataacagttc cagcaaactc aagtggttat
    19981 atagcattaa cagtggattt gacacaagaa gttatacctg gatctattct tccagaatcg
    20041 gaagaatatg aatggactaa taatcaagtc aagctagaat ttataacaaa agttataaaa
    20101 ggtaatttaa acaatggtga taaggtctat aatttaccac tatgctcagt taattctact
    20161 ggatcaactg tttcaatctc aaagatatcg gatagttacg agctgactct ctctaaagga
    20221 gaaattttgt ggagggggac tgcgttaatg catgatactc aaactgtcca accttcaaaa
    20281 aaaatttggc agacagtttc aggttttttg ttagtatggc tcccatacga aaacgggcaa
    20341 gcaattgagg atagatatgt aactacgcct ttttataagg agcgtataac ttttactaat
    20401 gttttaggag aaattgtttc aggatttgat gactatcaca aaaagtggtt tagtaaacga
    20461 ataaactata attcgaatac taatatattt acaggtgcag caagcaatgc aagtggagat
    20521 aacgcaaata tggtgcttag gtatatagtt tctttttagt taggaggtat ggaagagtgg
    20581 caaatttaga aattaaatta tctgcaaata agagacagcc ttatctacgt caccgtgttg
    20641 ttggtagagt tggtgatggg gggctcacaa caatcaatgt acaacttctt gaggaagatg
    20701 aaattacacc ttttgtaatt aatctaaacg gtactttgaa atttgtgggc gaagtttcaa
    20761 acggtaacta taccgaggga gaaccagaaa taatcgattc gactaatggg ttaattagtt
    20821 acacgttcac taagtcaaat ttcagcacga gtcatcaatt caaacaagca tattttgaat
    20881 atgtagatcc taacggcaaa aaagtaactt ttcagaactt catcatagac gtgttagaac
    20941 gagtagatat taattcggag caagcgaaat actatatttc ttcattggaa aaattacaga
    21001 gtgaaatgca aaccactttc aatcagttca ttagtgataa acaggtccaa tacgatcaaa
    21061 tctactcgaa atataacgaa ttagtaagat tgataaatga atcagataag caagtaaatg
    21121 atcgtattga taaggcaaat aatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg
    21181 gcaagctgaa aaagatgtac agtaacagca ttga
//
LOCUS       DACECS010000046        21200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.046, whole genome shotgun sequence.
ACCESSION   DACECS010000046 DACECS010000000
VERSION     DACECS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.046"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..215
                     /locus_tag="IWO31_001815"
     CDS             <1..215
                     /locus_tag="IWO31_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0302224.1"
                     /translation="ISLYQHHLTKSRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(350..865)
                     /locus_tag="IWO31_001816"
     CDS             complement(350..865)
                     /locus_tag="IWO31_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ0302225.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(1047..1658)
                     /locus_tag="IWO31_001817"
     CDS             complement(1047..1658)
                     /locus_tag="IWO31_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0302226.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1684..3135)
                     /locus_tag="IWO31_001818"
     CDS             complement(1684..3135)
                     /locus_tag="IWO31_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0302227.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(3159..3890)
                     /locus_tag="IWO31_001819"
     CDS             complement(3159..3890)
                     /locus_tag="IWO31_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302228.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3908..4303)
                     /locus_tag="IWO31_001820"
     CDS             complement(3908..4303)
                     /locus_tag="IWO31_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0302229.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4405..6042)
                     /locus_tag="IWO31_001821"
     CDS             complement(4405..6042)
                     /locus_tag="IWO31_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0302230.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(6077..7411)
                     /gene="gmhB"
                     /locus_tag="IWO31_001822"
     CDS             complement(6077..7411)
                     /gene="gmhB"
                     /locus_tag="IWO31_001822"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ0302231.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7453..8562)
                     /locus_tag="IWO31_001823"
     CDS             complement(7453..8562)
                     /locus_tag="IWO31_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302232.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8587..9822)
                     /locus_tag="IWO31_001824"
     CDS             complement(8587..9822)
                     /locus_tag="IWO31_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302233.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(9853..10983)
                     /locus_tag="IWO31_001825"
     CDS             complement(9853..10983)
                     /locus_tag="IWO31_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0302234.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10970..11743)
                     /locus_tag="IWO31_001826"
     CDS             complement(10970..11743)
                     /locus_tag="IWO31_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0302235.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11757..12212)
                     /locus_tag="IWO31_001827"
     CDS             complement(11757..12212)
                     /locus_tag="IWO31_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ0302236.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12411..12644)
                     /locus_tag="IWO31_001828"
     CDS             complement(12411..12644)
                     /locus_tag="IWO31_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302237.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            complement(12634..13740)
                     /locus_tag="IWO31_001829"
     CDS             complement(12634..13740)
                     /locus_tag="IWO31_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0302238.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13758..14519)
                     /locus_tag="IWO31_001830"
     CDS             complement(13758..14519)
                     /locus_tag="IWO31_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0302239.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14595..15593)
                     /locus_tag="IWO31_001831"
     CDS             complement(14595..15593)
                     /locus_tag="IWO31_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0302240.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15612..16265)
                     /locus_tag="IWO31_001832"
     CDS             complement(15612..16265)
                     /locus_tag="IWO31_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0302241.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16275..17285)
                     /locus_tag="IWO31_001833"
     CDS             complement(16275..17285)
                     /locus_tag="IWO31_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0302242.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17304..18554)
                     /locus_tag="IWO31_001834"
     CDS             complement(17304..18554)
                     /locus_tag="IWO31_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0302243.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(19900..20073)
                     /locus_tag="IWO31_001835"
     CDS             complement(19900..20073)
                     /locus_tag="IWO31_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ0302244.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(20416..21105)
                     /locus_tag="IWO31_001836"
     CDS             complement(20416..21105)
                     /locus_tag="IWO31_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0302245.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
BASE COUNT         6790 a         4576 c         2921 g         6913 t
ORIGIN      
        1 agatttctct ttaccaacat catttgacaa agagccgaaa aacagttgag actgtttttt
       61 cttccttaga aaaattaggt tgtcaaaatt tcaactcacg ttctgttaaa gggctagaga
      121 gtagatttga aagcatatta ctggcttaca gtgttctatt aagtcgagca caacgacgtt
      181 ttgaaggaac tttgagatat tctttaggat attaaaatta actagcacca cgggtatatc
      241 tatatctaaa tttagtttta tgtattcctc atagaaataa ccgagcattt gctcggttat
      301 tgttcttctt attttccagt atccttcttt tctccacagc tcattttaat caaaagctta
      361 atctttccac atttttgtct ttctcactta taacgaaaca ctgccgactg accggccatt
      421 caatatctac gctttcataa cttatacatt tggcagcatt agaattgaaa tcattatcta
      481 cgcaccactg aactagcgta ttgtcctcca tagtaagata gccatgagca tatccacgtg
      541 ggatatacgc acatttacca ttgctttcac tcaatgtgtt gacatacaca catccataag
      601 tggaagatcc ttgtcgaata tcaagtgcga tattcataat ttcaccatgt aggcacatca
      661 ctatctttcc ttgagcatgc tctccactct gataatgtag ccctcgcata gtatatttat
      721 tatgactaaa tccttgattt atttgcttaa tgtgaaactc aataccaatc gagaaaagag
      781 tctcattatc caatgcaaca gcagtatatc ctctgaaatc cgaaaactta attatatcaa
      841 acacttttac cataactacc accatgttat atattttacc aattttttaa ttcgctcgac
      901 ctcatttaaa gcaataaaat tccctacctc ttgaagatat aacaacaatg ttttagcaaa
      961 aatagatggc atgtaaagta ttcttgatga gattacacaa tcctgtctgc agaaaaatat
     1021 taaccatatt aaaattcaaa gcagttctat tttaagtcta tatacttatt taatcggtag
     1081 aatagccaat atagcaaagc accaattaag ccccctaggg tgttataaaa caaatccgat
     1141 agttgaaatg taccaagcct aaaaaacagt tgtgtaaatt ctatacttaa tgagaataaa
     1201 aacacatact taatcccaac atagatggtg cgtataatat cacatttaat gtcacgccac
     1261 cataacaata acatgcaaaa agggataaac agcatcgtgt tctccacgca ttctgttgtc
     1321 aatctgctct ctcctgtagc attatctata atatataatg accactgtcc aataacgttt
     1381 gaaagcgggt tcatccacat atttcgatta agcaaggttt taaataatat caaagtaaaa
     1441 taaaacgcaa gaaaaaagag tctgcgaaat ttactagatg ctttaaaata tctcaaccag
     1501 attaatacag aatcttttaa gtttttagga tctcgagagt tatcaactat aaatagatac
     1561 cagtacataa acagatacgc tgttaatacg gcaaataaga acggctgata taatgctgtt
     1621 aatacattta ccgttatagc agaaataata tctatcattc tctatctctc tatgagcaaa
     1681 tgtttatttg ctcataattc tccttactaa aagtagcaat cgttttctaa atataattgc
     1741 agcgacacaa gcaattgcta taattaaata ttttatgaaa tcaaatttat taattattga
     1801 aattaggatg ccaccagcta tcgtgattgc agaaatcaaa agtattcctc tgacatcata
     1861 tatgtttttt ccatccaata gttctttgca aacttttcta taaaaaatat aatggatgac
     1921 gcagaaaaac atatatgaga atacagttgt gtatgctacc gcctgatacc catataactt
     1981 tataaaaaca taatttagta caatattcat aattgcacac aaaaccgatg gaataacgac
     2041 agtgagtttc ttttcaaagt attcctgtac tctagcaaac agctgaaaca tatagttgaa
     2101 ataaataccg atacatatag gtacaattaa attaacactg tcgatatatt tctgcccacc
     2161 aaatatcaac acaatttctt tactaaatag cattaatgca aaaagcatag ccgctaccaa
     2221 accaagaact tcattagctc tttttgatag taaatcatat tctcctgact tgattttttg
     2281 atactgataa ggtgcaaaag tggaattgac agaatttgta acaaggttaa ttagagcacc
     2341 aatagaatat gccacgctgt aaatagcaac atctgacgaa ccacataagt aatttatcat
     2401 aattttatca gaaccattca gaataaattc agataaatag tgtggcatca acgatacaca
     2461 aaatactact gcgaaattcc acacttcatt atccttaaaa tccttacatc ttctaaatac
     2521 cgatatatac acagccacgc caacaacacc agtcactagg acattcatta aggctctcgc
     2581 ctcagcggta ggagatataa acagaaccgc taaacagcca actacagatg agccaacagt
     2641 aactgcaagg gtagctacta ataaagtctt ataatcatat atatatcgtt tgtgtaaaga
     2701 ccaacattgt atttctgcct gaaatataaa atatgagaac atacagacaa ctagaactgg
     2761 tgtcaatcct aaaatttctg ccaatatatc cttaataaaa atagcaataa caaaccaaaa
     2821 aatagtcagt actaaagtca atccggatac tgtcgaaaga atatgttctt gattatcata
     2881 ttttacataa agattcatca tggctttgaa caaactaagc gatgtaatca tgagcaataa
     2941 ggattcccac gatagataag tgctgtataa accgtattgt tctgtagaca aaactctagt
     3001 aaaaattggg gttgtaatca ttgaaacccc tctctgtaaa aaaccacata ccataaacca
     3061 taatgcagcc ttaacaggaa gtggagtatt cttatatctg cttaatgttt ttacaattag
     3121 attattactg gtcactgcta ttttctccta aacactattt attcttgatt caaaaacaca
     3181 ttgagctctt ctggagtacc aagcacatga acctttttcg catctactat gctaatagtc
     3241 acctttaacc ctttttttat cataaagtta tataatggtg ctatatattt ttcattcttc
     3301 tccatattag aggagtctgc ataatactct tcatataaag ctttatacag ctttgccgat
     3361 gagaaataat acgcaccaag agtacaatta tcagaaatgc gaactttttc gcggacttct
     3421 attacttcat ttcgatcgtt aagctttgca aaactccaat gatcgccatc agcatgaaag
     3481 catggaatat gtccatctcc acaaatatct tcgtatttca tctcatttga ttccacgtat
     3541 gtatcaatat tgtaaaccat aatctcattg tcttgattgc aataaggaat agccaacata
     3601 catgtagttg cttgaccatc tgtcatatag tctatttcaa tcacttctac gttttcaata
     3661 ccaaagtaag cacaatgctc ctttataaaa gctgaagcgt tgtcttcctg acgaacaaca
     3721 aaaatatact tatcaacatt tttattgtat ccaaccaaac tatccatggc ccaatcaaat
     3781 aaagttcttc ctttggcatt aatcatatat ttaggaaccg cataaccaat ttttcgaaaa
     3841 cgacttccca aacctgccat cgtaatgata actgtcatct ttttttccat agaactaacc
     3901 tccttattta gaatcttttt tttgaactgt tgcagatttc atttaattca tctacagagc
     3961 atctcaaaaa ctcatcaggt ctaaccgttc gatcatctac atagaagcct ttatgccccg
     4021 gccaaggctt accatacagg atttcatcat acggaatgtc ccatttatcc aaccacgcca
     4081 agagcacttt tgcagtatta gcatttatca ttcccaaatt tcctttatag gaattaacat
     4141 tgcgtgaggt gaacaatgta attttagcac cattctcctt atagaatctc agtttttcaa
     4201 ccatattctc atacggcacc aaatcctcat atcgctcatc cttcttcttt atcggacaca
     4261 aagtgccatc gatatcaaac acaaaagaat attcactata catttttctc ttcccttcta
     4321 ttgtattaat taatataatc gtgccttaat gcctacattg ctaaaacagt attgtaaccg
     4381 ttattcatta aggcacaatt tatattagag tataacttta ttacttactc ttgcaagaag
     4441 ttccatacct gtagcaagca tcactatttg gtgatcaaaa ctttcaccgt gtaatggaat
     4501 catactaaga aatagcaacg cttctatcaa tacaatctta tcaaaatcgt ccttaatctc
     4561 gtccgaaaag accgacttaa acaattcaaa taaatcgtaa tctcttacac gcgtattaat
     4621 tgtataatct atcactgcac actcggtatc atattctaca gtaaatagat ccttgatgat
     4681 ataatcatat ttaccatcta aactatgaaa tagttttgct aattcatatc tgctgtcccc
     4741 ataaatatca tattttccaa attttcccct aggatcaata accttaacaa aggaacaatt
     4801 ggaatctacc atcatattag caaaacacaa atctccatgt ataatacaaa attcatttac
     4861 atcatagagc atacttggga taagattctt tagaagttca attacaccgg ttaaagaaat
     4921 aaacttacga ccattaattt gaatttgttt ctcaaaaaaa gctgaaaatc ttttatcttc
     4981 agacaatttt tgaaatctct gcatggtttt tcgcaaatac atatcttcca atgactctct
     5041 aatcttttca tcttttaagg tgtatcgctt aaaatcgcta cagataaact ttatacggct
     5101 aaacagatca tgccactggt tatattttaa atcaccatat aagtataact catgaaccgt
     5161 atgatatgca taatattcca tatcaacata gggagctacg taagatgtag aataatcaaa
     5221 aattctaggt cttacatatt caatatcagc aggaagtttt aaataccact ttatttctcc
     5281 aataaattta tccttgtcat cgctagtttt cttcaatatc cctcgatctt tatctataga
     5341 gatatgatta aactctcgtg ctttaacttc caactttgta ttgtaatatt tatcgatgtg
     5401 tccaatatca aaccactttt ctgttcttac ggcctttagc ggacaacgtt cgctgtatag
     5461 ctgtaaagcc cgataaaatg agtttattcc cccctgtttc cgactctcaa ctgcctctct
     5521 taagcaactc ttcagaagtt ttgacgaaga aaacataaat acgccaataa ataatttttc
     5581 acgtttatcg gatacgtaag cttcctcttt atcgtggata tcagtaatta ttccttcatt
     5641 aattttaaag tatgtccaag tactcgacac aaaatcttca gaataataga atgtatccat
     5701 ttctatatca gacatactct ccataacaat tgtatctgcg aagttaatta gaacaggctc
     5761 atcttcatct gataacccaa aatacacggt ttgccctaga tcccctaatt caggaaggtc
     5821 acatatatga attctggaat catgatagtt tttcagtctt ctatgaacct tatccgcttt
     5881 ttcataacag ataatatcca atgaatcgca gattgcatta tactgattat acaaataatc
     5941 aaagacaatt tttttattaa ttggatagat aattgctggt aacattccta tactctgcaa
     6001 ttctttttca acaacttttg ctgctggaat taataccttc atctaattac tccttttaca
     6061 agtttcacta aaccaattat tgcatagtgc ataaatgatt tttaacaaat tctaacaatg
     6121 aacagacact atagtcttgg ccataattag caggcaaggt gatgtcttct gtaatcagta
     6181 ctgtcttaca accagcattt tttccagcaa ttacatcact ttcactatct ccaaccataa
     6241 atgattgact taggtttatg ttaaaatcct ttgatgcttc taataacagt ccaggttttg
     6301 gcttgcgaca attgcaatca atttttaatt ctataacctc tcctgcatat cctttatggg
     6361 gatggtgcgg acagtaataa ataccgttta aaaatccacc agcatgccct agcattgttt
     6421 caagcttatt gtgaatacta ttaagctcat caacagtaac ttcacctctt gcaattacag
     6481 gctgatttgt tataacaata cacagaaaac ctagagaatt aataatttta attgcctccg
     6541 atactccatc aattaactca aattcttctg catttctcaa aaaaccaacg tatttattaa
     6601 tcgttccatc tctatctaaa aagattgctt tttgtggatt ggatagattt ttaccaacaa
     6661 taattccctt ttgaaagtcc ttaattacag ctctatatcg gtccggtgtt cccatatctt
     6721 taacatactc tggagaatcg tagcaataca tacgccctgt gttagccaat ggtttcagaa
     6781 tcattctatc taaatcaatt ttgtatggta gccccccacc ttcaactatt gatttattta
     6841 caatttccat taatggattt tctctcaaca ccttcggatt tataacatga agacctgcat
     6901 ttactcggtt tttataatat tcaggtcttt gatcttcctt cgctaaccat tgaagcactt
     6961 tcttattgtc atcggatata attaatccac tatcatacgg atgtgagtta ggatgcgtaa
     7021 acaaagtaac atctgcgtta ttctcaacat gaaatctaat catctgcctg aaatcaacat
     7081 caaatactgc atcagcattc aaaagtaaaa acggttcatc acccagttca tccaacaaaa
     7141 aatacaatgc acctgcattg ccaagtggag ttttttcaat aaaatatttg attttaattc
     7201 ctaattttga accatctccg aaatgctcct ctattttttc atgcaaatag gaaacagtca
     7261 aaataatatc attaaagcct tgatctctaa gcgaacacaa ttctcgttct aaaactggaa
     7321 tgccatctat aggaatcatt ggcttaggaa tatccggaaa caattccgaa attcggcttc
     7381 ctcgtccgcc cgccataatt actgttttca taatatgccc ttccctaaag tactttttac
     7441 tataaaagtt aattactctt cccaaacaaa gtccatatat ttttcatatt ctgctttgta
     7501 ttcgtatttt ccagtgtaca aattcaacca atttataaaa tcaaaattat agcagtgaag
     7561 tgcatcttgg ctatcatcaa tataatacgt accataaata gtattatttg aataacgacc
     7621 tacatattta tgccagtaca aatcaatttg atttttgtta atagtaataa catggttttt
     7681 tacaaactcc caatatgccc taatagagca ctcctcattg ccccccattc gtgaaatata
     7741 gcttcgaaga agctgaactt ctggagctaa attattttca gatatctccc ttcttgagac
     7801 tcctctagac acagctccct tttctctcac atcactagga atcgagaaga ggtttatcat
     7861 atcatcaaca tcaccaaaat acaggaagtc tgacaaacaa tacgggaaaa acatgtctcc
     7921 atacggcatt gatagtaaca gtaatcgctg attttgtatt aatttccaat catcttcgtt
     7981 attaacagga tactgttcaa gtaaactgca gaaaaacgcc attgcatttg gatggtatat
     8041 tctttgatct gttctagttt tacaaacata ctttgcacca agctgctttg ctctgactat
     8101 tccaggcaaa gtgtttccaa cttgataatt aatatttaag tgtccaggct tttcaggttt
     8161 ctcactacaa ataactataa ctccaagatc ctctatcctt ttcaatgcct taatatcttc
     8221 gtccttccat gtcgaaagaa taattaaagc atttggataa catttcttat agtaggccag
     8281 agtattataa gagaaatcat cctttaaaca tagtggcccc tgcattacaa ttgctacatc
     8341 tttgcaacaa ttaactaaac ccatcactgg aacatcagac atgtcctttg agcgcaattg
     8401 aaacgctgca taaacttcag aattttcttc tactgttgca caatcagcag tcaatttcca
     8461 agatacgagt ttacttatat tatgaccaaa catgttccta aacacatatg gtaaattttc
     8521 gtacttcttc aatatgttat ataaaatctc tttcttattc atgagatgca ctcctattaa
     8581 tatttcctat tctatgttag ccacacttct acattcttga tagatgtatt ctataaccgc
     8641 taagcataca ggaatagctg gagtaaatat cggataaaac cagtctatat taatccctgt
     8701 tagcaagaca tttaatgtaa ttccaaatgc taaatattga tagccagtca tacccacttt
     8761 tgatgcaagt aatgctgttt tgaacccttt tataataaac agaatatcaa atataagtat
     8821 acctacacca tattgagtaa ttgtatagaa aaatccaata tcagataggt gtcctctaac
     8881 tcctcctggt aatataaacg taaattcctc accagtaaga ataccagtac cgaacaaagg
     8941 cgattgcaaa aatttaggaa taaaaaacca aactgaagca aatcgagatt cagcagatcc
     9001 tgcatattca cctgtactgt ttgtgaaagt ttcaacaaat gttgagaaca tggtcgaatt
     9061 aataaatatg actacaagca acccagctaa aacccacatt gcaatttttc ttttcgaaga
     9121 acctttctta aaaaggatat agcaaccaag tacaataatt tgatagacaa ggacagatct
     9181 agaaccatga ataacagcag aatatgccag tgcgctagca atcagtaaat aatattttac
     9241 ttttcttcta gctacaactt gtcgctcaat catatacaaa gcaatcggta ttattataat
     9301 tgtaaaacat ggtggattaa ttcttaaaaa gcctccacga taccaatttt ccggggcaca
     9361 ctcacgatat atgttttcat agagaagtac tcctgaaaat ttctgaataa gtgaaataga
     9421 tgttctaaga atgtatgaaa gaaatgttac accacatatc gtatttagca ttgaatccaa
     9481 attccacgct tggttaatta aagtacagaa caatggatac gccaataata catagtataa
     9541 actcatactt tccctagatt taacccatat tgtcttatct gagtagatgt aagctgctat
     9601 tataacaaca caaatcagta aagtgcagaa atttaaatat ctattttttc taaatgctgg
     9661 taatgaggct attaacaaac atactattgc tagaatgact gtcacaagtc taacgttcat
     9721 cgtttctccc aaccagccgc ccaaaggctt caatatgctt gatgagataa tcattagaaa
     9781 gaccattaga aactgtatga ttttccattt actaatctgc atgttaatta ttattccttt
     9841 ctaattgttt tactactcat tcaaaatttg tttgtatgcc tcgtatgtga gttttgcaga
     9901 tgccgcccag gtatacttgc ggtttacaag ctcatatata tcatcctcta ttttgctttt
     9961 ctcagctggg ctaaggttca catactttac aattgtgtct gctaggctct tagaattcaa
    10021 attttcgaaa agtaacccta tctttccttc ctcggtttct tccataaaag caggtacatt
    10081 actaacaata gttggtaagc aataagtgta tgccataaga agcgctccac ttccatagat
    10141 ttctttatat ggaaatattg cggcatctgc atttctgaac atactgtcca tttcttcatc
    10201 agaaattctc tctggcttat atagaacaca atctgaaacc tttaaatctt caatcatttt
    10261 tttatagtca gtactatcct gtttaggatg ttgttgacct gctatggtaa aatgaacttt
    10321 ctttcttaca tccattggaa gttctgtgat tgcattaagc agtacatcaa ttcccttata
    10381 tggacgaatt tgtccaaaca agataaattc aaacttatct ttatcatacg ctctagatat
    10441 atgtttaaca tctccataga tagcatgtgg acaaacatat gactttgcat ttaattctgg
    10501 gaattcagca tataaatgct gttctgttac tcgattgtga acaatgaaac catcacaatt
    10561 tgaatatata ttaaagtgct cttggcgatc cttttcacga acctcatgtg gtaacacatt
    10621 atgaactgtc ataattaatt ttttatagtg tctcttacat aaaacgtaca atccgccttc
    10681 atatttgaga cttttcatca attgcaaatg aacaatatca taatcaccaa atcttatttc
    10741 cttccaaatt aagaacaagc ttttcagaaa aacaagtgcc gaaaacactt tatttgtact
    10801 tttatgatac aaaatatgtt tgctcttaat tttaggatct aaaggaggaa catcacgctt
    10861 acacagcaat gttaaatcaa catattgatt aagttcttta gaaagatcaa acgaatacca
    10921 attctctggc atttggcttt cacacatcaa taccttcata atactcacct tataaccaat
    10981 caacagaaaa ttctgctttc agttttcctt taattttacg ttttgctata aacatttcca
    11041 gtatctctcg cattttcgtc tgatattttg tcgttttacc ataaagcgag tgattgtccc
    11101 ctgttcctaa tacatatact gctgctttga atggaagagg ttttaatgga cgcccttgct
    11161 cttcacatgc tggaacaacg cccccatgtc gttttctaat gatccatgga ttatcatcac
    11221 aatttgctgt cataacttcc ggatatgcct caggcaggtc ttccactgca tattttacta
    11281 tacacgcact tcctgatgag aactttggta gaaccttcat ataatcgtct ccaacaaagt
    11341 acacgtatcc cgttttgaca taaaatccat tttcctgggg atgggaattc acatattccg
    11401 ccaaatgaga acttacaaga tcatctgcgt caaccatcat agcgaaaccg ccgcccaact
    11461 ctcgcaaacg cattgcgaga gcatgtgttt tataaccctt atcaatcatt ttttcgtctt
    11521 gatttgtggg aataggggca tccttttcgc taacttgaat aaattcaact ctgtcatcat
    11581 attcatgcgt gagtttagga atttcatggc aagcaacaat gattttgaaa tcaggatcag
    11641 tctgattata acacgaccat aatgttcggt taaataaggt actaaccatc tcccaattgt
    11701 ttgctgcctt cgcactcatt aaaggaataa caaaatacaa catacaatct ctccttttag
    11761 taacgattca aactacaata atcatttaca tcaatatctt ttttcaatac tctagccgga
    11821 acgcctccta ccgaacaatt aggttcaaca tcattaatga ccacagcatt agcccctact
    11881 attacattgt cccctattgt gatattacct aaaatttttg cgccagtagc gatatataca
    11941 ttgtttccaa ttgtaggcat accaggctta ttggatctac caccagtcag tacattcgtt
    12001 ccgattataa cgtgattacc tatttttgta tcttttccaa ttacacaacc tatgccaccg
    12061 tataagaact tacaattatc tccaatttct gtttgatatg aaattgagct attatgaagt
    12121 acataaatta gtgcctttac aatcttggca aaaaactcca ttttgtgtaa gtaaaaccat
    12181 cgttctactt tgtataattt atatgcattc atgttttatc tccccatctt tttagcaggt
    12241 accccccaca cttcgtaggg tggaatagac ctcaatacca ctgtatttgc agcaactaca
    12301 gcaccttccc caacgcataa ttctgtgtta gatttacaaa gaattttagc acccacacat
    12361 aaaacagcgt tcttttttac tcgaatgatg atacgttcat tactatctgc tcaaccacac
    12421 gtcacattct gatagacttt aacaccatct tcaaggattg tgtcagaatg aatcacagtg
    12481 cctacagaat tatgaacaaa ttcaacagag ttccctatct gtgcaccttt accaatacaa
    12541 caaccaagaa ttttggctat ttttctcgtg caattatcaa ctatgtttaa agcatatcga
    12601 ttcctcaatg ccattaattt cgttacttcc atatcattta tcaccttcca acatgctaag
    12661 cataatttct cccgactttg accatctaaa ttctgtagtt acacgctgat atgctgcatc
    12721 tatgatatcg ttggtaaatc gtccgtcttt taagaatgtc aaaatttcat cgtcagattt
    12781 agccaacatt ccatatctat tatccaaaat aatattctgt gggccaggtg cattaacagc
    12841 aataatcccg aggcgatagt acattgcctc tagcaaaacc ataccccaaa tttcagtata
    12901 gtttagattt acaaatacat ccgataagca atataactcc caaatatctt tgttaggaat
    12961 acgctcaatt atttttactc tattctgcag tccaagtaaa tttatctgct gcttcgtttg
    13021 ctcgagtaaa gcaccagttc ctaccatcag caaataatta tgctcatcaa gtttattaaa
    13081 taaactaatc atatctaacg gtcttttatc cttttcaaac cgtcctacat ataaaataac
    13141 acgaacattg cgaggaatat tccatttttc tctgagagta gtaaaatcta ttgcttcgta
    13201 attcttatgt aactgttcaa aatccacaca aacaggagca agtttcactt tatttactcc
    13261 taattgtact aatttttcag aaactcgatc atttttggca acacacccat ttttcttata
    13321 aattttataa tttcgatgcg ccaatatttc aataattgtc tttatatatt tattgccact
    13381 ttgacttcgc gttatcccat tataaggtat atattttatt ccatttttta cagtccatga
    13441 gtatacatga ggtactgata gttgaaggtc cgcaaattgt attagaacat ccgcattctt
    13501 atcaaaattc tttgttgata agaatccatt tgatcctaca taacgacact tgatatagtg
    13561 aatcatcgcc gtattgctta attgctctgt tccaacttcc tcatcacttt ttacactttt
    13621 atatacaata acttcgtggc ctaattcagc taaccctcga gctaatccta tttcctgaga
    13681 attataggac cccttcttac caaagttatt cgtcattgta actactataa gtattttcat
    13741 ctatttttat tgccccccta cttagattca atagtatagt agatatactt tatacctccc
    13801 aagatatacc gcctccatag tctacgaggt tcctttacca gtctaaataa ccactctaga
    13861 ctagcttttt ccatccattc aggagctttt tccaaagtgc ccgcaaaaaa gttaaagccg
    13921 gcgcctacac cgaacattac tgtgttatgg atcttcttct ggtgttccca catccacatt
    13981 tcttgcttag gcgcaccaat accaacccac agaaaatctg gatttaagtt gttgatttcc
    14041 tcgcaaatct tgctatcctc ttcttcattt aacggacgaa atggtgggga atacattcca
    14101 acaattttga ttcccgggta atctctttct aggtttgcac gaagcttatt taacacttcg
    14161 ttatcttttc caccatagaa ataatgtgtt tttccctgtc taacgccttc ttcaaaaacc
    14221 attcccataa tatctggtgc agcacatcgt tcacaggtaa atcctttgcg ccgtccaatt
    14281 ttaacaatag gcataccatc tattgcagcc attgtagcct ggctatacat ttcagcaatt
    14341 ttgggatttt cctttgccga aataatggcc ggcactccca cgagatcaac agtcgcactt
    14401 accggagatg ttggcatgga aaaaacttcc cttacaaact cgttctttgt agtagcacaa
    14461 acacctacta ttccacacac agtgaaatca cgttttcttt tatacatatt cttttccatc
    14521 tcagtacatc cttactcata tgttcttcct ccgcatcctc taattacacg gaggaagaac
    14581 agcttatata cttattaacc cttaatctgc ttttcaatcc aattgtagga ttcacgaaga
    14641 ccctcttcca aagaaacagt aggctcccaa tccaaaatct ctttcgcctt agaataatct
    14701 gcgcaacgag ccttatcacc ttcaggctta gaagtatcaa agaaaggctt gatgtctttg
    14761 ccagaaattt caatgatctt atatgcaatt tccttgatgg aagtacagtg agaagggcca
    14821 atctgaatat gaccatgacc ccaaccctta tccagggcga gcaccaatgc atttacgata
    14881 tcattgacat gaatgaaagc acgtccctgc tctccgctac cccacacatt aaaaggctct
    14941 tcaggattat tgattgcttt acgaatcaga gcaggaataa cctgactacg ttcgccaaaa
    15001 tcagtaggtg tgccataaac attatggagc atcaggatac agcacggaat tccggtttct
    15061 ttttccaaat accccatctc caactggccc atcagtttgc tccagccata agcagactca
    15121 ggaagagcag ggaacaactc ctcttcacgg agaggctcag gattcaaagt attctgtctg
    15181 gtcagaggga aggagcatac ggtaccaaca tagataacac ccttgatttt ctccttgcca
    15241 gccttacggc aacaatcaaa aacattagaa ttgatcagat tattgattcg gaacaattcg
    15301 ccctggttct taaacacata gtcaatgcca gccacaacat cagccagatg aactacatac
    15361 tcagtcgtac caataacctt tttagcctgc tcataatcag tcagatcagc attgaagaaa
    15421 tgagtatcca ggtcgatgac aggatgccca tcttcgtcgt tcaggtactc caactttcct
    15481 ctccacagat tatcagctac ataaacatcc cagcagcctt ccttcaccag tctcttaaca
    15541 agattggagc caatcatacc gcaaccaccg gtaaccataa ttgtcttttt catttaacat
    15601 tcctccaaat tttattcgtt tccaaagaat ttatcttcca gcatcaaaca ccaacaatga
    15661 tatatcggca agtgatactc ttgaatcatg tatgtttcaa cttcaggagc cttaacacaa
    15721 acatctgcta ccgtggcaag ttccccaccc tttgcgcccg tcaagccaat aacttttata
    15781 ccgagagccc tagcaacaac agttgcgctc ataatattct tgctatttcc ggacgtcgaa
    15841 ataccaagga atacgtcacc agatctacca aagccaaaca gctgctgtgc gaagacaccc
    15901 aaaccatcga catcattaat ataagcagta gacaatgctt cgtgtgcgac caaaggaatt
    15961 gccgttaaac cacactcgag atttttggct aattccggac ctcttacagg gtcaattgca
    16021 actaacttat cggcaaattc cttacttaca ggacgtggcg tcttaaaccg cttcatcaac
    16081 tcgccagcaa tatgttccga gtcagcggct gatcctccgt ttcctgccac aagaagttta
    16141 ccaccattca aatagcattc ctccataatt aggtatccat cgataatgtc ctgtttaatc
    16201 gtctcaagtg taggatatcg tttaatcaat aagtcaatat gtttattaag tttttccgct
    16261 aacattgtta ctccttatcc attctgtttt tcaatataag tttccggcgt gtagtgaata
    16321 actctagttc caccattttc aaattcaaac ggaatataca tcaaatcacg catagcccaa
    16381 cgaactgcat cctgtttctc aggttgtaca tagaaaacca agaaacctcc accacctgca
    16441 ccgagcaact ttccgcctaa agcacctgca gccataccct tttcatatag ttcatcaatg
    16501 cttcctgtac taattgctcc accagtacct tttttcaatc tccaagtaac atcaagctgt
    16561 ctaccgaaat catctaaatt tctgttctta tcagtaagaa ttgcctcagc ttcatctaca
    16621 agctcataca tctttttcag tcttactctc ttatcctctg tgccactgac gttgttagcc
    16681 ttttgaacat cagaagaaaa tctagtgaat ccggtaaaga acatcatcag attgttgtta
    16741 agctgctttt tacgttcagg cgaaataatg acaggcagca cctcataacc atccacattg
    16801 aaattaattc gattgaaccc accataagat gcggcaatct gatcctgcca tccaccggct
    16861 tcattacaca gaactctttc aagataaatg gcttcatcag caagtttctt cttgtcagca
    16921 tacttaccct ccagtgcata aaaagcattc aacataccaa ctgcaaaaga actggatgta
    16981 cccagaccag agcgagcagg taaatcagct tcgtatgtaa gccgaatttc atgcatatcg
    17041 agcattttca tcgcattacg aatcgcagga tgctcaatat cttcaatgct agttactctc
    17101 tcagtacgag aataagataa ctcagcgctg taatcaaaaa atctaggcag atgtctcaca
    17161 ttcacataac agtacttatc aaaagtagta gaaagaactg caccgccatt ctctttgaaa
    17221 taatcctcca tatccgttcc accgccaaaa aaagacatac gaaacggtgt ttttgtaata
    17281 atcataaata ctctctccta atcttagtct ctctggaaga tatcacgggt atatactttt
    17341 tccttaatgt catcaaggca agcatcgtag cgatttgcta taacagaatc acacattgcc
    17401 ttaaactgtt ccaaatcatt cacaacctta ctgccaaaga aagtctctcc atctttcaag
    17461 gtcggctcat aaatcacaac agttgcacct ttagctttta cacgcttcat aacgccctgg
    17521 atagaactct ggcggaagtt atcagaattg gatttcatgg tcaaacggta aacaccaatc
    17581 acaacaggct tttctttgcc agcatcatac tgatttgctt ctgcgtaatt gtaataacca
    17641 gccatctgaa gtacacgatc cgcaataaag tccttacgag tacggttaga ttcaacgatt
    17701 gcctcgatca gattttcagg aacatctgcg tagttggcca gaagctgctt ggtatccttc
    17761 ggcaggcaat agccaccgta accaaaggaa gggttgttgt aatgagtacc aatacgagga
    17821 tcaaggcaaa ctccattaat gatctgctga gtgttcaagc ctttcatctc tgcataagtg
    17881 tccagctcat tgaagtagct gacacgaaga gccagatatg tattggcaaa cagcttaaca
    17941 gcttctgctt cagtaaagcc catgaacaat gtgtccacat tctctttgat tgcgccttcc
    18001 tgtagcagtg tagcaaaagt gtgtgcatcc tcaaccaaac gctcatcatc catatcggta
    18061 cccacaataa tacgcgaagg atacagattg tcatacagtg ccttactttc acgcaggaac
    18121 tcagggctga aaataatatt cttgctacca gtctttacac gaacagaagc ggtgtatcca
    18181 acaggaatgg tggacttaat aaccataatg gcatcaggat ttacttccat gaccagccta
    18241 ataacagcct caacagcgga tgtgtcaaaa tgttgagtgc ggctattgta gttagtagga
    18301 gccgcaataa caacaaaatc cgcagactgg tatgctgcct ttgcatccaa tgttgcggtc
    18361 agattcagtt ctttctcagc caagtacttt tcaatataat catcctggat tggagattta
    18421 cggttattaa tcagttcaac cttctctgga atgatatcca ccgctgtaac cttatggtgc
    18481 tgtgccaaca gagtggcaat actcaaacca acataaccgg tgccggcgac tgcaatgtta
    18541 tactgtttct tcataatatt tcctctttcc accactgctg tggtcactac tttcttaaca
    18601 aaatgctgtt tgtgaacagt aacctgctat ttatataaaa cagcccgaaa gctgtgttca
    18661 ttcatactta aaacagccag ctcctacgcc ggcaaattca atttttcata aatcccctac
    18721 ccgtttaggg ttatattgca agtttgagtc atttttattg acctcgtttg gggttataat
    18781 acattttcgc attgaaatgt acctacattt tccactcaac cagtcaccaa aaagtttata
    18841 aattcactta ttaccctcta ttcttactcc aaaggtatgt ttaacatcgc tttattgacc
    18901 tattttcacc ccaaatggaa gtttgtttgg tacattattg gtctattccc accccaaact
    18961 gtctattttg agcgagtttt tgttttttat cattgcacat tttcaaggct caaattctaa
    19021 gcggatttac tggcagggag acattggtaa atacttgaac aatttcacgg ctttcatcat
    19081 cacatcctac gataataatt ctaattggat aaagattatc attaataatc tactttcacg
    19141 taaaaattct gaactaaata aaatctattg ataatatttt tttcttccac tttttgtata
    19201 tcctacaggt atagtggatt taattatccc caatgcctct gaagtaacag acatcactaa
    19261 ttcaataaca cattcactac catagtttgt aggaacaaaa atgacaacaa aatctgcttc
    19321 cttatgtgat gtttttccat ctgtccaaat tttattacca atgaaatcta gtttaaaatc
    19381 cctcttagaa actagttcac tctatatttt tattcagttt cattagttct gtttttttgt
    19441 cttcagtcac catatacata tatatcttaa ttatctatcc atttcatcct acgcattctt
    19501 gcgtaaatac aattcactct gttgagctga tataaatcat tggtggtaca aagataaaat
    19561 gataactgtt tctatacctc caaaaattcc agtaaatata tctatagata gtttcaaaaa
    19621 aaattgtctt agtttcataa tatttaagat agtggtacat actttataac aacgtacttt
    19681 tccaaatatt attttccgtt atgaacatcc aatatattac aaagtttaaa agtttagatg
    19741 aactataatc ttcatttctg cacagtggat aataaattct tatagataaa aaatagcctc
    19801 ttctaaagtc acaaatacct tactcccaca ttttaagtaa acactctgca gcattctctt
    19861 tgttataaaa tgatagatat tcctcaattt tatctcttat tatgttatta tcagttgata
    19921 ccattccatt aatgtttcag aataattaca atattttggg accggataac tcccttttgc
    19981 tcgtaaccac tcatatccgt taggattcac taaaagttta ccgtttattt ttcgattctt
    20041 tttttgaacc gtcccatgaa cataccgatt caacaagcta aattataaaa attggtgtgc
    20101 tgtcattatt ttttggaaaa tttattaatg gctatcaata tacttttgta tcatactaaa
    20161 tagctcgtca gttcctatat cggatatacc aatattaaag catttagatc attatattta
    20221 agagtggtat catcccaaaa tttcgaatat tttcttttat ataagaataa tgatactgaa
    20281 ttgccccatt cacctttctt tttttagatt ttctacaagt gattcaaacc ctccataatt
    20341 acagttatta cttttgaaac gataatatga acatgtgtaa tcttttttat taacaatcca
    20401 tcattctatc cctaactaat atgctccatt gggcttgaca ataacataaa acgtttttaa
    20461 aatcaacctc aagtcgtaca ttaaacttcg atgactaata tactctaaat ccaactcaac
    20521 cattttatgg aaactaacat tatttctatc actaacttgc cacaaacctg aacaaccagg
    20581 ttttacatac aagcgttgaa gatcatatct cgtgtactca gctacttccc tctccaaagg
    20641 tggccgtgga ccaattagac tcatatcgcc ttttaaaaca ttccatagct gtggcaattc
    20701 atcaatgctt ttggcacgga taattttacc tattttagtg acacggggat cttctttcat
    20761 cttgaacatt gcaccttcta tttcattttg atcaagtaat tcttccaatt tttcttcagc
    20821 atctaaacac atcgaacgga atttatacat tttgaatgtt tttccatttt taccaacacg
    20881 tgtatggcaa aaaaatatcg gacttcctgg ctcttctttt ctgattcgat aagctacata
    20941 gaacatcaaa ggagaagcta aaatcaaacc aataatgctt cctacaatat caatcattct
    21001 tttaaaaaat aaatagatat atcgtttttc aactttttct ttatccaacc aaacttgatt
    21061 aggatgtact tcactaatac gagataataa atcttcactt tccatacttt ccctctctcc
    21121 tcatttcctt tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc
    21181 tcttcataga ctggataact
//
LOCUS       DACECS010000047        20818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.047, whole genome shotgun sequence.
ACCESSION   DACECS010000047 DACECS010000000
VERSION     DACECS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.047"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..317
                     /locus_tag="IWO31_001837"
     CDS             <1..317
                     /locus_tag="IWO31_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0302246.1"
                     /translation="GKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(741..911)
                     /locus_tag="IWO31_001838"
     CDS             complement(741..911)
                     /locus_tag="IWO31_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0302247.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1105..1284)
                     /locus_tag="IWO31_001839"
     CDS             complement(1105..1284)
                     /locus_tag="IWO31_001839"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302248.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1671..2783
                     /locus_tag="IWO31_001840"
     CDS             1671..2783
                     /locus_tag="IWO31_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0302249.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2861..3154)
                     /locus_tag="IWO31_001841"
     CDS             complement(2861..3154)
                     /locus_tag="IWO31_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0302250.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3322..3636
                     /locus_tag="IWO31_001842"
     CDS             3322..3636
                     /locus_tag="IWO31_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0302251.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3657..5636
                     /locus_tag="IWO31_001843"
     CDS             3657..5636
                     /locus_tag="IWO31_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0302252.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5649..5975
                     /locus_tag="IWO31_001844"
     CDS             5649..5975
                     /locus_tag="IWO31_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0302253.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5991..6509
                     /locus_tag="IWO31_001845"
     CDS             5991..6509
                     /locus_tag="IWO31_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302254.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6509..7861
                     /gene="celB"
                     /locus_tag="IWO31_001846"
     CDS             6509..7861
                     /gene="celB"
                     /locus_tag="IWO31_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0302255.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7945..9450)
                     /locus_tag="IWO31_001847"
     CDS             complement(7945..9450)
                     /locus_tag="IWO31_001847"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0302256.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9542..10132)
                     /locus_tag="IWO31_001848"
     CDS             complement(9542..10132)
                     /locus_tag="IWO31_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0302257.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10311..11957
                     /locus_tag="IWO31_001849"
     CDS             10311..11957
                     /locus_tag="IWO31_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0302258.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12006..12725
                     /locus_tag="IWO31_001850"
     CDS             12006..12725
                     /locus_tag="IWO31_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0302259.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13204..14151
                     /locus_tag="IWO31_001851"
     CDS             13204..14151
                     /locus_tag="IWO31_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0302260.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14163..15083
                     /locus_tag="IWO31_001852"
     CDS             14163..15083
                     /locus_tag="IWO31_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0302261.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15124..16581
                     /locus_tag="IWO31_001853"
     CDS             15124..16581
                     /locus_tag="IWO31_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0302262.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16926..17540
                     /locus_tag="IWO31_001854"
     CDS             16926..17540
                     /locus_tag="IWO31_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0302263.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17533..19257
                     /locus_tag="IWO31_001855"
     CDS             17533..19257
                     /locus_tag="IWO31_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0302264.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19254..20705
                     /locus_tag="IWO31_001856"
     CDS             19254..20705
                     /locus_tag="IWO31_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302265.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6715 a         3512 c         4172 g         6419 t
ORIGIN      
        1 atggaaaact ggcgatagat ggcttagcac caggagcata tcagctggta gaaactcaag
       61 cgccaacggg gtatgaattg gatgcgacac cagtaacttt caagatcgaa aaagaacaag
      121 aagtggcgat ctttttaaca aaagagaata gaaaagttgc tgatagctca gatataagag
      181 gaaacaatcc aacaagcagt ggaaataagc atcttccaaa aactggagaa accatcatta
      241 gccagtttat attgtcaata ttgggtgtgc tacttgtttt catcagtata aaatttagaa
      301 agaaacgaaa tatatgagct aaaataaaaa attgaataag catagttttt aaaatcaact
      361 agtacccatc aattgaatgg aaactaccga ttttagaagg ggtatacaaa caaatattag
      421 tatcggagta gagaaggttg aacccaacca gtaaataagt aaagacaatt ttgtgctgcg
      481 ttgagaagtg aaaaataaac ctttgtagta atactagtta ataaaaaata tacaaaaaca
      541 ctgcgaaaat cattcttgag tttcgcagtg ttttttgcct aatgactcaa gtttattggg
      601 taatactttg attttctttc tacaaataat cagcgatcag ttgtaatttt ttctctagtt
      661 ttaatggctc tttgtcaata aggagaaaaa caagtcgggt cttttgtctc ggaaagcttc
      721 aaccaactcg tgaataaagg ttacgcctcc ttgaaaggcg tggaaaaacc aagtaatcga
      781 tcaatcacca atttcgcatt tggtagaaca tgtttggtga gctgaaagta ggtggcgttc
      841 atgtctgtaa ctaagaattc tatttcttta ggattagtgc agctaaggaa atagtttgtt
      901 aatcgaggaa ttttttgtgt aggtaaattt tctcgattaa tttttctgtc tcgccatccg
      961 cggaaataaa actcagttta tcctcaacgg aaacgtgaga acgaaattca caaccctcaa
     1021 cactcaagac agaattcttt tggaagactt tggtaaatag cttctcaact ctttcaatgt
     1081 gcgaataacg gttgtcaaag atacctaaca acttttcgaa ataaaaaata acgacacttt
     1141 ttcagtcagt aaagaagtga ttttatgtct gacatgattt gaaatcgagt gccttggacg
     1201 gataaaataa ctttgagcgt tccaatggct tcgacagttt ttacaggtat agcgctgctt
     1261 ttttaggcgt ataaccattg gcatatgatt atattattca aagtggacaa tggtttcctt
     1321 cttcttgttt ttcaccacca ttaatttcct atttccatct accacagcag aaccacaatt
     1381 tctacagaca catggagcag ggaagagaac tgcagcaacg atcaatgttt ttttcttctg
     1441 taaggtttcg taagagacct ctgtaatcat caaatttttg tctattgatc tcagaatttt
     1501 tttgatagaa ccattcataa agcatatcgt tctctcagtt gtttattttg tggtgattta
     1561 atcatacaag aaaacgatat gtttttttat aaaaacatga ataggaaaaa tagttgacaa
     1621 gaaaatgaca acgccatatg atgttccata gtatttacaa cctgtcatag atgactgtaa
     1681 aaaataaaag taaaaagaaa ggacgacaac aggtggattt ttatcagtct ctacaactag
     1741 atccgtttat tttaaaacaa aaaatacgag aagcagcttc taaaaaagaa aagtgcatgt
     1801 atatttgttc attgttttta cgatcattat tcatcgtatt gtttgccatt tgttttatca
     1861 ttataattac aacattattt gaatcgaagc ataaaccata cgctgtggtt ttattctgta
     1921 tgttgatgag tattcgtttt gttgactttg gttataagat ttcccattcg atcataggtt
     1981 tagcaatcgt catgttctca ctattagtag caccgtatgt ccagctgatc aaatggtcag
     2041 ttatgggcat gttgattcat tttattttac taagcagcat attaatggca accgctagtg
     2101 atcccaaaat ggggaatgct tctttgtacg gcttttcata tctctttatt gtttattcgt
     2161 tacctaaaga ttcgttgaac aagaatttct ttacacaaac aagtagctta ttatttttgt
     2221 ttttttgttg gttttcagtt ctcctttaca gaaaacatcg agaaaagaac aaagacaaaa
     2281 gcttgtttaa agagattttt ttgaaaggca tgtattcgca ggaaaaaatt tggatgctca
     2341 tctatgcatt tggcattagt cttttgatcg ttgcaggaga gtatgtccct tttcagcgat
     2401 tgatgtgggc agggtttgca ttttcttcta ttgtatctag ctatggattg atgtcgattg
     2461 gtttcaaaga acgagcagta gaccgaatca ttggctcatt gattggatgt gttttattta
     2521 ttggtatcag ccaatttatt ccttttaact gggtcggtat attaggtggt ctagctttag
     2581 gtgtttgttc tacctataga tataaaacag tttttaattc ttttggtgct ttggcaatca
     2641 ctgccagttt attcggggtt ccaggagcag taacgatccg catttttgag aatatccttg
     2701 gggtatgttt aggaatcatg tatattgggg tgactgaaat acttatccga aaaataagag
     2761 aaaaacacgg tttgaatcat tagatgagat cgaataaaaa ataaggaaaa agtgaagata
     2821 cacatggatg ataaattgct atcattccat gtgtatcttt ttacggtaaa tcagtgaaac
     2881 gttcgacttt catttttaat ttatactttt tacggagttc gtttatttga gccatcattt
     2941 cggatttgtg atgcttttcg attgcttctt catctttcca acggtcgatc agtaaaagag
     3001 attctggatc ttctagtgga aagtaatagt catatttctc gtttccttct tctgcacgaa
     3061 tcgctttgac aatgtttctt tcgaccattt cttctgcaaa ttttctggct tgtccatttg
     3121 tacttgtata caaaatattc actgtgatac tcactaaatc ccctcctgaa aaaattctat
     3181 cactagtata tcacggtgcc agaaattatt attcaagata tgatacacat tttttccgca
     3241 atgctacgga aatatatatt ttgaatagaa aacgcttaca agatatgctt ggtttataaa
     3301 aataaaagga gtgattgatt tatgaaaaaa gcattgatta tttgtgcggc aggaatgtca
     3361 tcctcgatga tggcctcaaa gacaacggat ttctttaagc aaaaaagaga agaggtactc
     3421 gtagatgcag tttcagctac agaaggcgat aatatgatta agaatagtga ttttgattta
     3481 tttctgatca gcccgcaaac gacgatgttt ttggataaat tcaaaaaatt aggagaatct
     3541 gttggaaagc ctgttgttag tatccctttc caagcgtatg tcccaatccc tacaggcatc
     3601 cagaagttag cagaggtgat cgaagagaac atatgatgga ataggagtgg atgataatga
     3661 aacagaagga aaaattgctg ttgagatatt tgattgatca caagaaggag tatgtcacca
     3721 gtcaacgttt agcttctgaa ttgttattat ctgatcggac aatccgtaat tacttgcagc
     3781 ggatcaaaga gttagtagaa aaaaacggag gtaagatcat tgctaaacca ggttatggct
     3841 accaactgca tattttacaa agactgacat ttgacttgtt tttgagtcgg caagaaatag
     3901 tacctggata tgctagagaa ccgcaaggat tttatgagtc cgaagatcga caaaagtata
     3961 tattgaacca attgcttttg gaagatgcca ttctttttat ggatgatttg gcagaagagc
     4021 tttatatcag tcgttccagt ttgacaaagg acatgcaaga aatcaaggag cgattgattc
     4081 catattcact aaaaatcgtt tctaagcatg gtcaagggac atggatcgaa ggggaagaaa
     4141 gaaatcgacg gcattttatc atggatactt tttttggaaa ccattatgga aattcattga
     4201 aagaatattt agggcaaagc cgttttttcc cagacatcag ttttgaagag ctagtcatta
     4261 tcattctcga tgaaacaaga gaagctaagt taaaagtttc ggattttatc atacagaatc
     4321 tgactttaca cttggctttg gggatcaaac ggctccgaga agggtttgaa atcaaggaat
     4381 taggcatcga acaagaagtt tctaagcgag tagagtatca agtggcgcag cggatcgttc
     4441 aacgtattga ggtaatcgca ggtgtatcgt ttcccgaaga agaaattgct tatttgacat
     4501 tacatctcat ggctaagtcg aatcacaaag aaaatcaaaa tgatcaggaa ctatcaaagg
     4561 aattagcgaa agttctgaaa agattgtctc aggaatttca tttttcatta gtagaggatc
     4621 tccagctttt caatggacta ctcgatcatt tgtcaccgat gatgattcga ttgagccgag
     4681 gtattcgcat tgagaaccca ttgactgagg agataaagaa agaaaatccc aaagtctttg
     4741 acgcagtaaa aaggcatttc agcaacatgc cggctttgaa aaactatacc atcaatgaag
     4801 atgaatgggc ctatttggct ttgcatctca tggcggcatt ggaaaaagaa agagctgcgc
     4861 ataaactaca tgcattgatt atctgtgcaa cgggttacgg cagtgcccaa ctattaaaga
     4921 atcgcgtagt cagtgaattc ggtaaaaata ttacagtggt aagtgtaaaa ggctattatg
     4981 agatcaatga aacaacttta aaaggaatcg acctgattat ttcttctatt gatctttcga
     5041 caatgttttt caaaattcca gttttacatg tcagtatttt tttgaatgaa gatgatgttc
     5101 gtaaaatacg aaaggtagtt ggagaatcta taccctatca ttcctcagat agaccgatac
     5161 cagcattttc tctacagcaa aaaaagcaga tttacaccga acagttgtct gaaaaatttt
     5221 tcaagactta tacgtacgaa ccgaaaaagg aagaaattct tcatgatttg ctgcaaaagc
     5281 tgagcataaa tgaagaagaa ggatatataa gagaaatgcg aaagcagatc cagcaacgtg
     5341 aaaatatggg acaaatcata ttcagtgata cagtggcagt cccacatccc gctgttccag
     5401 ttggaatcga aacaaaggta gccgttgcat tgattccttc tgggatgcag tgggatcaat
     5461 atcaagaaat tcattttgtt tttttagttt cgccttctta tgtcgaaaat gaaggaatta
     5521 cggtcgtgac taaagcaatc gttaggttag ttgaccagct aaaagtacag caggaaatct
     5581 tagctgaacc tatttttgag aatttcagca gtaaatttat caaactgatc gaataaggag
     5641 gaaaacagat ggctgaaaaa ataaacagtg aaacactaca agtgacttct tttgatatca
     5701 ttttccatag cggaaatgca cggacactga tacatgaagc atttcgctcg atgcgtaaag
     5761 agatgtttga agaagtagaa gagaagttgc gacaagcaaa tgaagagatt cttcatgcgc
     5821 atcagtcaca aactcaactt ctgaaagaat atgccagcgg gacaaaaata gaaatggaaa
     5881 ttatcatggt ccatgcacaa gatcatctga tgacgacgat gacattattg gaagttgcac
     5941 aggaaatgaa ttatttgtat caaaaaaata aatagaaagg aaggatcatt gtgcaggata
     6001 cagtaaaaag gaaaatcgaa ttagaaaaaa agcaacttag tttgaaaaac atgtatttca
     6061 atcgttatct aatcgtacgt tacctgactg cctttttctt tttcatgaac atgcaatgga
     6121 tgattctgct tctatcagct aaaagtttag gaagtagttt accgatgatt ctgattttag
     6181 ctattttgcc tgcagtagga gaacaagtaa agctttatcg aaaacaccaa acgaacgttc
     6241 catggacaaa gtggtatttt ttgttccaga gtgtctttaa tttattagtg attccagtgt
     6301 tgtttacctc aggatttaca ttcttgtatc cttttatggc aaataacaat ggaggacagt
     6361 tatttgtttt cattcttgtt gtaagcggta tttttgccag tgcatttata cagtatcgtt
     6421 taaagaaaat ttcactcaac caagaccaac agtatatccg tattaaacaa tatgaaaaag
     6481 cgttatattt aggaaaggag aataactaat gtttgatttt ttgcagaaat acctgatggg
     6541 acctatgggg aaaattgcgc aacttaaaat cgttcgggct gtcatggcag cggggatggc
     6601 ttctattcct ttcacgattg taggctcgat gtttttggtt ttaaatattt tgccattacc
     6661 gttcccattt ttagaaggat tttttaatgc aacttttttc aaagttagtg acttgtacat
     6721 gatcgtcaat acaatgacga tggggatttt atctatttac tttgccattg tgtttgctta
     6781 tgaattaacg tcaattgaac gagatgaaca aaaattaaat gttaatccgc tcacgggggc
     6841 attactatct gtttttgcat tctttatgtg tatccctgaa ttgatcatga ctgatggtaa
     6901 aatcagcttg attgcttcta tcaatgagac agaaactgta gtaaatggta ttaggatggg
     6961 ctcttttgtc gaacgtttag gaacttcagg aatctttaca gctattatta tgtcgattat
     7021 tgcggtcgaa ttgtattgta tgtgtgtcaa gagaaattgg gtcataaaaa tgccagatgt
     7081 tgttccgcca ggagtttctc gttctttcac tgctctgatc ccaacatttg tcattgcgtt
     7141 tgtggtgatg atcatcaatg gattattagt tgcactagga acggatatct tcaaaatgat
     7201 tgctatccca tttgggtttg ttacgcagtt gacgaataca tggcttggtg tcatggtgat
     7261 ttatttcttg atccatgcat tgtggatcgt agggatccat ggagcgaata tcatcacttc
     7321 tttcttgacc ccgatcgtat tagctaatat ggcagctaac gcacaaggcg caaattatcc
     7381 gttggcagga gaattcaaca attcttatgt aaccgttggt ggatcaggtg cgactttagg
     7441 attgatcatc tttattgcat tcctcgcgaa atctgatcag ctgaaagtgt taggaaaagc
     7501 ctcacttgta ccaggattat tcaatatcaa tgaaccaatc attttcggga tgccaatcgt
     7561 ctacaatccc tatttagcgg ttccgttttt cttagctccg atggcttctg ctacactcgc
     7621 ttattttgcc attcggctgg aaatcgtcaa accgatgctt gcacagatgc cgtggccttc
     7681 accagtcggg atcggagcat ttgttggtag tggtggtgat tggaaagctg ctgtgttagc
     7741 agtcatttgt gcaatcatcg cattcattat ctggttccca tttatcaaat tctatgataa
     7801 taaattatta aatgaagaaa gagaaaaggc agcagaatta gctagtgaaa agttaaaatg
     7861 atttctaaga aaaaaggaac tgtgacaaaa aatcacagtc cctttttttc ttagaaatgg
     7921 gaaaccacta gtatataaat accattattc ttcaatcaac cgcttcacga ttgcataatc
     7981 cccttcataa ggttcatcta caccattgat tttttgatag ggatccacgc cttttcccgc
     8041 aataacgact gaatcttgcg gagcgctttc tgctaaagcg cgtcgaatcg cttcaggacg
     8101 atccatttca taatgaacga tcaaatctgg attagtaatt gcttcattga tttcttccgc
     8161 gatttttttc ggatcttcaa atgctgggtc atctgccgtc aaaaaggcga tgtcagctgt
     8221 ttctgatagc acttttccaa aatcatgtcg acgggagatt gctttgtttc ctgggctgcc
     8281 taatacaaca attacacggc catctggatg ttcatttttg gcaaatttca gcaatgtttt
     8341 caagcttaga taattatgtg catagtccac atagacatgc gctccattgg attggatcaa
     8401 ttgatccatt cggcctggta cacgagcttc ttttaatccg tcttgtgcat cttgactgga
     8461 ggcacctgtt agtgctgcgg caatgatcgc actggctgca ttttcttggt tgaatcctcc
     8521 agccagcaaa atatcataag cagtatcgca gatagctaag cgatcttcat gagaagataa
     8581 agtaaaaccg tgagtacctt tatctgaacg gatcacttga taattgcttt cttccgaacg
     8641 accataagtg atcgttggaa tctggaatag atcggctgtt tccctaagta aatcaaaata
     8701 gtcactttca tgatttaaaa caactgtttt tgaattcaag atcaattgtc gcttgcaata
     8761 gaaatagtca tcaaatgtcg gatgttcgat cggactgata tggtcaggcg agatattcaa
     8821 gaagattccg acatctaaag tcaagccgta tacgcgttgt gttttgtaag cttgtgaaga
     8881 aacttccatc actagatgag tcataccatt ttcaactgct tctgacatca tacgatataa
     8941 atctaatgac tcaggcgttg tcaaatggga tttgaaatat gttttgccat ctaacgttgt
     9001 attcatagta gaaagcaatg ctgttttttt atttgttgtg tgatccaaga ttgcttttgt
     9061 aaagtacgca gcagttgttt taccttttgt tccagtgaat ccgatcactt tcaatttatt
     9121 ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt gctttacgga tatccgttac
     9181 gatgattcct actgctgaat tttcgtatgg ttgttctgat acataatatt gaataccctg
     9241 ttcgattgct gaatccaaat attcttcttt gaaattcatt cctttacaga aaaacaaagt
     9301 agaagcatca gcttttcttg aatcataact taactgaaga aattcagctt tttctggtac
     9361 atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat agattttctt ttaatagtaa
     9421 atgacggatg tcttctaaag agaagctcat tgattgatcc ctcgctttat tcaatttctt
     9481 ggtttattat agcacactct ttttaatata tcgtttgttt ccgctatgac aaaatccagc
     9541 tttattcaaa ttttcttgac acaggggaca agcggatgag tattttcata aacaatgaac
     9601 tgactgtaat agataggaga acgaatagtg tctccaacaa tccaaaaaca taataattca
     9661 aaaagacgat aagactattg gtaagtaaaa gtccttgcgg cattggaagc cgaaaaaatt
     9721 tgtggaaaat aatcggcggt atcgttatac cgccatttga ggccccgact gtgtacaatg
     9781 tgtaaacccc aagactgaac aaaagacttc ctgaaatcag tgaaaagaaa tgtgaggaga
     9841 tcatttccac ttttggaaca atctctaaaa caacaggaaa aagcagactt cccgctacag
     9901 tcttaaaaaa tactgatttt cctaatagga cagcggctag aagcagcatc ataagattga
     9961 tgctccaaac agtaactgag cgttggatag aaaaggccgt ttcaactaaa tgtccgatgg
    10021 cgcccactcc ggcggaagct atatcgtgag ggagcaagaa ccaattcaat ccaactgtga
    10081 taaagaaaag acctaaaaat ataacgggta gtttttttat ttcttgtgtc attgatatct
    10141 cctataaatg ttagccataa aaggctgtat atttctttac tttaccaaaa aaacgaaacc
    10201 gaattcaaaa aaacaattgt tcttataaaa gttatataaa atcgattgcc agtttctgtt
    10261 ttaccaatta aaatgatagt aatgttttgt agaaagttgg aatctgaccg atggataatc
    10321 aaagaaaacc agcattagat caattaacac cgcaagaaaa aatggcacga ggctctgctt
    10381 ggatgaccgc cagtaatatc atttcaagat tgctaggcgc tgtctatatc attccctggt
    10441 atgcttggat gggcgaaaat gctaaggctg ccaatggatt attcaatatg ggatacaata
    10501 tatatgcatt gtttctactg atctctacag caggaatacc tgctgcgatt gcaaaacaaa
    10561 ctgcacgcta taattcattg aatgaatatg gcacttcacg acgattattt atccgtgcat
    10621 tacagatgat gggcgggtta ggcctgttgt ttgctttgtt catgtacatc gcctctcctt
    10681 ggctagctca tgcttctggt ggcggagaag aactgattcc aacgatgcgt tctttgagta
    10741 tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg gtatttgcaa ggcaatcaag
    10801 aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt ggcacgtatt ttttatatgc
    10861 tattggctac attcatcatc atgaaagtca tggatggtga ctatgtgacg gcggtcacgc
    10921 aatcaacttt cgcggcattt atcggtatgc tagcaagtat tcttgtcctt ttgtattttt
    10981 tgaagaaaca tcaagcgtat acatcggcgt ttattcacta cagcgaaaac aaaataaata
    11041 ttgcaacgaa ggaattgttg ttagataccg ttaaagaagc gattccgttt attattgtgg
    11101 gttcaggggt aacgatcttc aaattagtcg atcagttcac ttttatgaag atcatgtctg
    11161 catccaccga gtattccaat gcacagttac tggatctttt ttcgattttt agtgccaatc
    11221 cagataagtt gacgatggta gttattgcat tggcaacatc gatcgcgtct actgggttac
    11281 cgttgattac agaagctgtt acgttgaaag atcgagtagg tctggcaaaa ttgacaagcg
    11341 gaaatttaca gttgttttca ttttttatgt ttccggcgac atttggtgtt atgttacttg
    11401 cctatccgct aaatacatta ttttatacac cggattctct aggaagtaat gtactgatcc
    11461 aagcaagttt tgtaggactc ttcctaggac tatatatgct agtttccaac atgctgcaag
    11521 gaatgtttga aaacaaggcg gcaattaatt atttggttat tgggtttcta gtgaaactcc
    11581 ttcttcaata cccagccatt cgtttatttg aagtatacgg ccctttattg gcgacaatga
    11641 tcggatttgc tgtgtcttgt accttgatct taaagagaat ccatgaagta gcccgcttta
    11701 aaccaagttt tgtctggcgc aggacattgc tgatctttat tcttaccttg attatgcttt
    11761 tggcttcgtg gatcacaaag atgatttttg gtgctttttt aaacgaagat agcaaattcc
    11821 agtctctatt gttgattctt gtagtagcag gtataggggc tttagtgtat ggttatttag
    11881 ctttgaaaat ccgcttggca gaaagactac taggaccagg aatagcgcgt ttacgacata
    11941 aattaaaaat aaaataagtg aatcagaaga gctagattga tgctcttttg aaatggggga
    12001 aacgcatgcg tttagataag tttttagcag atatgggact tggcagccgc aaagaagtca
    12061 aaggattaat caaaaaagga acgatcgaag tcaatggaca agtggtgaaa agcgataaag
    12121 cgcaagtaaa tgaattcgaa gatgcggtta cttatttagg agaaatcatt acgtatcaaa
    12181 aagattttta ctacattttg cataaacctg caggagtcat ttctgctact caggataatc
    12241 atgatcaaac cgtaatcgat ttattggaag accaggatta tcgggaagat ctttttccag
    12301 tcggccgatt agataaagac acagagggcc tcttgatttt aacgaatgac ggggtgtttg
    12361 cccatcagtt actatcacct aaaaaacatg tggagaaaga atatctagct gaggttagcg
    12421 gtataatgac agaagaagat atccgtttat ttgcagaagg actgattatt gatggcgatg
    12481 agcagacatt gccggcaaaa ctccaaattg atcaagtaga tcaagagaca gaaacttcaa
    12541 ttatccgtct gattttgcat gaaggaaagt ttcaccaagt taagcgaatg gtcaaagcag
    12601 taggaaaaga agtcttatat ttaaaaagaa tccgtatggg aaaattttgg ctgcctaaag
    12661 atttgaataa agggcaatat cgacccatga ccaaagaaga gctagaacaa gtgagagaaa
    12721 aataattgaa tggatagcta aaagaataag cagagtagtt gtagcgccat ctgaaaagca
    12781 gacttgtccg tctgactttc ggatggcttt tttgtgaaat ttcggcttat ttccgtagaa
    12841 gctacttctg gaacaccgtt tgttcggaat aagggactgt gacaagactt ttgttacagt
    12901 ccccattgtt ccttgtgact cagagttaga tagtaaggac acgatctccc taatactcag
    12961 gccaaaacgc tgaattcaca acctctgata aacggtgttc aatcagctga ccctcggtat
    13021 tagctcaaaa aacggcaaaa tatgaggaac tattttcccg tttttttctc taatactcag
    13081 gccaaaacgc tgatttcaca acctctccta tactttgttt cattttccct atactttgtt
    13141 tatttattct cattattgaa agcgctataa ttacgttgcg aatgaatcga aaggggtttt
    13201 tagatgaaaa aacgaaaggg gttcaatcat ttttttatga atctatggcg ttataaagca
    13261 cttgtcctga tggctgtacc gggtatgctt tggatgattt ttttctttta catacctgta
    13321 atggcgaatg tcgtagcatt caaaaatttt catatttcag cagatgggtt cttggctagc
    13381 ttgagggaaa gtccttgggt gggttgggaa aatttcaaat ttttattttc ttcggatcaa
    13441 gcatttctca tcactaaaaa tacaatttta tataatgtag catttatcct tttgaatctg
    13501 ctgatctcag tcgtatttgc aatcattatg agcgagttga gaaataaacg actcgttaaa
    13561 gtttatcaaa cgatgtcctt actaccttat tttctatctt gggtcattat cagttacttt
    13621 gtttacgctt ttttaagtcc tgacaaagga atattcaatc agtggatcac tggacatgga
    13681 ggagaagctg tcaattggta taatgaaccg aaatattggc cgtttatctt ggtgttcatt
    13741 ggtacatgga aagggattgg ttataacagc attatttact ttgcatctgt tatgggaatc
    13801 gatccgactt attatgaagc tgctatggtt gatggagcta gcaaatggca gcagatcaaa
    13861 catgtgacga taccacaact tattccacta atgacgattt taacgatttt agccgtagga
    13921 aacattttcc gagctgattt tggcctgttt tataacattc ctaggaattc cggtgcatta
    13981 tatgatgtga ctcaagtttt ggatacgtat atctacaatg gcctgacatc cacaggagat
    14041 tttggtatga cggctgctgc aggactttac caatcagttg tcggctttgt cttgttgatg
    14101 atcacaaata cgatcgcccg acgttttgat agcgattctg cattattcta aggaggaaaa
    14161 aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc tttcaataag acggctaatt
    14221 ttttctctaa tataatgatt gcactctttg ctatttcctg tattttgcca tttatatttg
    14281 ttatcgccat ctcttttacc gatgagtcca tattgtcgac tcacgggtac agtttttggc
    14341 cagaaaagtt cagtatattt ggttatacgt acttgttcga ccagatgcag gataaaattt
    14401 tccaagcgct tttcgtcact gtacttgtta ctgttttagg tacactgatc aatagcacgg
    14461 ctacttcttt atatgcttat gcgatttcac ggtcaaactt tccattccgt cgttttttta
    14521 ccgttttttg tttgattacg atgttgttct caccagggat ggtggccaat tatcttgtta
    14581 tgacaaatct tcttcagtta aaagatacga tttgggcatt gatcttgcca atggctgtca
    14641 gtcccttcaa tattattgtg atgcgcacat tctttaaacg ttcggtctct gattcgatta
    14701 ttgaatcggc acggatcgat ggagctagcg aactgcgaat atttgttcag atcgttttac
    14761 cgttagctgt tccaggtatt gcgacaatca gtttatttgc cgcattgggt tactggaatg
    14821 actggttcaa tgctttacta tacatccaag atgataagct agttccacta cagtatcttt
    14881 tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa ttcaggagca ggaagccaat
    14941 tgacaggcgg attagcctca gttccaggag aatctgccag aatggcgatc gtagtcatct
    15001 cgacgttgcc tattgcgatc agctatccat ttttccagaa gcattttgtc aaaggtctaa
    15061 caatcggcgg agtaaaagaa taaagaaaca aaaatataaa tcataaaatt aggaggaaga
    15121 aaaatgaagt tatggaagaa actagccttt acaggtgttt cagcgttgtt gttgggaaca
    15181 ttagcagcat gcggaggttc tggttctaaa gaccaggcag aagctagcaa ttcggacact
    15241 ttgcagatgt atcaaatcgg agataaacca gataatttcg atcaattgat ggaagtagca
    15301 aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata tcaattacat tggctggggc
    15361 gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg aaaattatga tatcgccttt
    15421 gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg
    15481 cctaagtacg ctgaaaaagc ttataatgat ttagatgaag catatattaa aggaaatctg
    15541 gtcaatggaa aattatatgc tttcccagtc aatggaaatg tctttgctca acaagtactt
    15601 acttttaaca aagctttact agataaatac gatctttcaa ttgatggaat ccaatcttac
    15661 gctgatgcag aaaaagtctt gaaggaattc catgaaaaag aaccaaacac ggcagctttt
    15721 gccattggac aaggcttcaa agtccaaggg aactttgatt atcctttagg gaatacgtta
    15781 ccatttgcta ttgatctgga tggagatgat tcgaaaatca tcaaccaata cgataatgaa
    15841 acgtatatcg gactgttacg tacaatgcat agctggtaca aacaaggact cattccaagt
    15901 gacgctgcaa cgagcaataa ggattatcca ctagaaggaa atacttggct aatgcgtgga
    15961 gaaacacaag gaccgtatga ttatggcgat acaattttga caaatgcagc aaaacaagaa
    16021 cttgtctcaa aagcaattac tgttccacta aaatctacag ctcaagcaca aatggcaaat
    16081 tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag tagaacttct agggctgctt
    16141 aacagtgatc cagaattatt gaatgggctt gtttggggga tcgagggaga agcatgggaa
    16201 aaagtcgatg gttcagatga taaaatcaaa ttgctagatg gttatcagcc aaatacacat
    16261 atgtctgctt ggaatacagg aaacaacaag attctatata cacaagagtc tattacggat
    16321 gacatgattg ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc gattttagga
    16381 ttcagcttca acactgacag tgtcaaaaca gaattaagta atatttctaa tgtcatgaat
    16441 caatatttag atggattgaa tacagggact gttgatccag atgaaacatt accgaaattg
    16501 aaagacgctt tggacaaggc tggctatgat aaggtattga aagaaatgca aaaacaatat
    16561 gatgagttcc gacaagaata ataatgaata aagacaaagg ggttgtgaca aaagacgtgt
    16621 cacaacctct tgttccgaat aaacggtgtt caaaagccag tttctcggta ttaagctaaa
    16681 tatatgtaaa aatgtggaaa gccatattca caaaattttt cttactactc gaaactaagc
    16741 ggctatttca caaactcttg gtaaacggtg ttcaatcagc taaccctcgg tattcgctca
    16801 caaaacgaca aaatatgagg aactattttt ccgtttgttt ctctaataat caggtcaagg
    16861 tgctgattgc ccaacctttt tttgtataat gagaaagtat aaacttaatg tttggaggag
    16921 aagatatgtt agggaaggtt ttggaagagt tatttatccg gatctgggtc attataaaac
    16981 taaccttgta tttctggata tatacatttg ccggcgggat catctttgga ttaggtgcgg
    17041 catggaaaac agtcaatgaa ctattttatt tgtacggatt cgagtataaa gaaatcacaa
    17101 taaaaagagg ctggaatata tataaaagaa attttttaag aggcaatctg ctatttagtt
    17161 tatttttatc tggtactgct ttattgagtt acaatttata tttatctgtt caaatcaaag
    17221 ggctcatttt tttagctatt gattttattt tactttttgc cttgctctgc ctatttgcta
    17281 cttatcaata cagtatgatt ttagatagtg aatatacgat ctctgttcct aatctgctaa
    17341 agctaagttt tatttcggtt ttttccagct tttcgtcttt tctaaaaata attattggta
    17401 gcggtataat tttaggcgtg acttggcaat tcaaaggatt gatcctgttt ggtgtcattg
    17461 gtcttttgac ggtttggaat ggtacgatga caacacattg gcgtgaagaa ttagataaac
    17521 agttggaaag ttatgaataa gtttcggatt ctattttcaa aaggaacctt actaaatcgt
    17581 ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga tgactgctac cgttattttt
    17641 agtatccata cttgggaaca aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt
    17701 caaagcacaa gcagaatgct gaatgataaa acaaccttga gtaggatcat caaaaaccag
    17761 cttgtaggga attccgaaaa gatcgaaaat gtgacgacct acttaacaaa acccatagat
    17821 cagtatctga tgtatgtata cgaacagcaa aacagtaccg atgaattagt ttctttccca
    17881 aatcaaatca aagatctcta cgcaaattat gaagaactaa gtgcgatata tatcgtgttg
    17941 aatcaactac cggagtatta tgaatcttct agagaaaaca aaggtgggga aataaaaaag
    18001 gggacaccaa aattactaga cgccttttat atcaagttgc ctatcacaca aggaggagct
    18061 gagtcaggaa gtatttttat tggttttcaa aagaaagaac tagatatgat tttagaaaat
    18121 ctaacgtcat tcaatggact ctctttatac atgatttctg gaacgacaaa tcgactatac
    18181 acattccatg ataataaaat agctaaagaa acgtttgaaa gacaagaaat gatcatcaca
    18241 gagagtctga agaaaaattc acaattgccg attgacgatc tggaaaaaaa caattttgtt
    18301 caacaccaag aactgtctga tgattatcgt atattagctg tactagataa aaaaagtgta
    18361 aaaatcgaaa cgggaaaaaa tcttgccccg ctgatcattg gtatcattat tttagacaat
    18421 atactgctta tctttcttta taaaacattc aaacgatatt cacagcaagt atctatcgtc
    18481 atgcaggcca tggatcaaac agcagcaggg aatttagaga cgcggatcga cataacaaac
    18541 acggaatatg aactgaggga actttcaatc ggtatcaacg aaatgttgga tagcatcaat
    18601 cagtttgtag aagacatcta caaattagaa atcaaacagc aagacgcaca tatgcgggcg
    18661 cttcaagcac agatcaatcc acacttttta tataatacat tagaatatat ccgaatgtat
    18721 gcaatcagcg aaggaagtga agaattagca gatgttgtgt atgcgttttc cgctttgctt
    18781 cgaaacaata cgaatcaaga gaaaacgatc actttaaaag aagaactgga cttttgcgaa
    18841 aaatatgtct atttgtatca aatgcgttat cctaatcgag tagcctatca ttttatgatc
    18901 gatcctgatt tggaaaagat cgaagtacct aaatttgtga tccagccatt agttgaaaat
    18961 tattttaaac acggtattga ttttactcga ttcgataatg cgctaagtgt caaagtcttg
    19021 caggaaggca aacgggtaag gatcatcatc aaagataatg gaaaagggat gactgaaaaa
    19081 cggctaaaac agattgaaga aaaactgtct catcctaaag tggaacttca cggttctatc
    19141 ggtctgcaaa acgtgaatga acgtttacgt gccagcttcg gcagcagtta ttatatgtcg
    19201 cttgagaata atgagacagg tggactgacc gtttctatta cattcaagga gggatgagcg
    19261 tgtatcaagt gttattggta gatgatgaat acatgatttt gaatgggtta aaaaagataa
    19321 ttgattggca aagtttaggc tttcaaatag tagcaacagc agagaatgcc aaagaaggac
    19381 tagctgtact tgaacagcga cagattgatt tagtagtaac ggatgtgaca atgccagaaa
    19441 taaatggact ggaatttatt gaagcggctc aaaaagaacg tcataatttt gaatttatga
    19501 ttttatcagg ataccaagaa ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga
    19561 attatttgat gaaacccgtg aataagttcg aattggttga aagcctcaag aaaatcaaaa
    19621 cacggttaga tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa gaactgttat
    19681 tcagtcaatg gttgaatgaa gaattggatg aaataagtga agaagaaata ataggacaaa
    19741 taagtgagaa gcagcatagg atagtattga ttcaactggc aagaactcat gatactctta
    19801 tcaatagatg gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat tacggagacc
    19861 gtgtattgat cacgttgttg ttggaggcag atgaagttga tggcttctgt tgttttgttc
    19921 aagaaaactt tcctgatcag gaatggctga tcagtatcgg ggaagagaca gattctttgg
    19981 acaaaatccc tgaaacattc caacaagcga aagacaatct acagttgcat caattttatg
    20041 gagataaaga cgagcatgtt ttttacgcag tacaagcaga aacaaaagat caaaccatcg
    20101 atttttcaag tttcagacgc gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga
    20161 tcactgattt ttttgaacag tttcagttag cagccatgat gccggaagat atacgatatt
    20221 ccagtttttt actgtttatg gagattcaac gggaattgat tgatctggaa gatgaggaat
    20281 atttacaagg aatcgaaaaa atccatcagg caaaaacggt gcaagaactt cagcagctac
    20341 ttctgtcatt catcaaaaag catcagagac agaaaaagta cagtgacaat gtggaaaaag
    20401 taatcgtaat cctgcaccag cattatcaag aaccacttac actcaaagaa gtgtcagaaa
    20461 acctgcattt aaatgtcatg tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt
    20521 tttcagctta tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta
    20581 atcaaaacat caatgagata gcaagtgaga ttggctacaa caacaccacg tatttctcta
    20641 aattattcaa gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac agtggagaat
    20701 cataaaagaa aaagccaatg taatgaaata acttataaaa aattgtctag aaatctaatt
    20761 ttgggctgtt cttattactt gaggcaaggc agctttttca tgacctctcg ataaacgg
//
LOCUS       DACECS010000048        20725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.048, whole genome shotgun sequence.
ACCESSION   DACECS010000048 DACECS010000000
VERSION     DACECS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.048"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(177..1160)
                     /locus_tag="IWO31_001857"
     CDS             complement(177..1160)
                     /locus_tag="IWO31_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0302266.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1165..1812)
                     /locus_tag="IWO31_001858"
     CDS             complement(1165..1812)
                     /locus_tag="IWO31_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0302267.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1897..2529)
                     /locus_tag="IWO31_001859"
     CDS             complement(1897..2529)
                     /locus_tag="IWO31_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0302268.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2545..3096)
                     /gene="coaC"
                     /locus_tag="IWO31_001860"
     CDS             complement(2545..3096)
                     /gene="coaC"
                     /locus_tag="IWO31_001860"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0302269.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3093..3830)
                     /locus_tag="IWO31_001861"
     CDS             complement(3093..3830)
                     /locus_tag="IWO31_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0302270.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3827..4816)
                     /locus_tag="IWO31_001862"
     CDS             complement(3827..4816)
                     /locus_tag="IWO31_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0302271.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5074..5616
                     /locus_tag="IWO31_001863"
     CDS             5074..5616
                     /locus_tag="IWO31_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302272.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5633..6718
                     /locus_tag="IWO31_001864"
     CDS             5633..6718
                     /locus_tag="IWO31_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302273.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6730..7536
                     /locus_tag="IWO31_001865"
     CDS             6730..7536
                     /locus_tag="IWO31_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302274.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7537..8364
                     /locus_tag="IWO31_001866"
     CDS             7537..8364
                     /locus_tag="IWO31_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302275.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8361..9434
                     /locus_tag="IWO31_001867"
     CDS             8361..9434
                     /locus_tag="IWO31_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0302276.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9862..12558
                     /locus_tag="IWO31_001868"
     CDS             9862..12558
                     /locus_tag="IWO31_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0302277.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12635..13123
                     /locus_tag="IWO31_001869"
     CDS             12635..13123
                     /locus_tag="IWO31_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0302278.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            13135..13629
                     /locus_tag="IWO31_001870"
     CDS             13135..13629
                     /locus_tag="IWO31_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0302279.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13650..14459
                     /locus_tag="IWO31_001871"
     CDS             13650..14459
                     /locus_tag="IWO31_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0302280.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14456..15277
                     /locus_tag="IWO31_001872"
     CDS             14456..15277
                     /locus_tag="IWO31_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0302281.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15328..17427
                     /locus_tag="IWO31_001873"
     CDS             15328..17427
                     /locus_tag="IWO31_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0302282.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17424..18542
                     /locus_tag="IWO31_001874"
     CDS             17424..18542
                     /locus_tag="IWO31_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0302283.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18581..19459
                     /locus_tag="IWO31_001875"
     CDS             18581..19459
                     /locus_tag="IWO31_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0302284.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19650..19802
                     /locus_tag="IWO31_001876"
     CDS             19650..19802
                     /locus_tag="IWO31_001876"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302285.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6348 a         3574 c         4407 g         6396 t
ORIGIN      
        1 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa
       61 ttccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
      121 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat
      181 tcagttatct tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct
      241 tgtttgactg cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct
      301 agaaaaggcc cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt
      361 ctaggtgtca ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct
      421 atctcaggca cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct
      481 atagaagcta aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact
      541 ccccgacctt cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc
      601 aattcatctg caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg
      661 atgattttcg cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt
      721 gctcctgcat ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt
      781 tgataagcag tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca
      841 gggagaagga ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg
      901 actaatccgt atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact
      961 acctctccag ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct
     1021 cttttcataa tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct
     1081 tgttttggtt taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc
     1141 ttgattgaaa tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt
     1201 caactctttg ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac
     1261 tagagcggca agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt
     1321 aggcttagtt aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag
     1381 aatgacaaaa tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata
     1441 ataatctgtc atggactcag gccattttgc tggttcatct gcataaacag cactgaccat
     1501 cagatagcga gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa
     1561 gtctacttgc agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc
     1621 tttgaatgct tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac
     1681 attctctttt tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg
     1741 ggaacttgga gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac
     1801 aaaaattttc atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca
     1861 aaagagaaaa aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt
     1921 gaagtatctt aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc
     1981 aggtatacca cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt
     2041 tccgatgact ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat
     2101 cgtattggtc aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg
     2161 tttcattagt ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg
     2221 aggaataaac gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat
     2281 aaacggagaa aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat
     2341 cagtccaaat gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat
     2401 gattacagga atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg
     2461 cgtaacagct aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt
     2521 atttttcata agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg
     2581 ttcgatgatc gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag
     2641 agattctctt ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg
     2701 agtgatcggg ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc
     2761 cactggaagc gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt
     2821 gccgataatg tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc
     2881 gatatgattg attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt
     2941 cgacagagat tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac
     3001 atctacctgg gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga
     3061 aatacttcca gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc
     3121 tcagtaatca atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta
     3181 gataagagat agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata
     3241 aaatcagttt tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt
     3301 acatctacta atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa
     3361 gaaatgattt ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc
     3421 tttgaaacag tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc
     3481 gttgaagaag tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta
     3541 aaatcactga ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct
     3601 tctaattgta aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt
     3661 cttttcggtt tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca
     3721 gctaaataat cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt
     3781 acttgatcga ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac
     3841 ctacatttac tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag
     3901 gcaattgatc cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca
     3961 tctgttgcca taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca
     4021 actggatgtt tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat
     4081 tgccacataa agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct
     4141 cgccacctac tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct
     4201 ctggcagaaa ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga
     4261 cagactcttc ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg
     4321 ctatcgctgt actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg
     4381 tcataccttg acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa
     4441 ggatcatcgc ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta
     4501 ctttttgata ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa
     4561 gtacaagatc ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat
     4621 caatccctgg agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg
     4681 catctttata attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact
     4741 cagtaaatgg cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag
     4801 atcgaaaagc gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa
     4861 gcctactctt ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag
     4921 aatcaaacaa ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac
     4981 aaagggttgc ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt
     5041 ttactattag aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat
     5101 ttgcgcatcc aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa
     5161 gggtatattt cccagttgga acgagattta agttcaccat caatggaaac attcttttct
     5221 attcttgaag tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg
     5281 caagtggttt attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa
     5341 ttggaatggc tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt
     5401 gaaggtgcag gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta
     5461 ttagaagggg aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag
     5521 tcgatctatt atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca
     5581 aaagtattga ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa
     5641 accaatcatt tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa
     5701 agtaagtttt gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg
     5761 taaaacaacg atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta
     5821 ttttgaaggg aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt
     5881 ccaagattat gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa
     5941 gatcaagaaa atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt
     6001 ccagcttcct ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg
     6061 tgtagcgatt gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt
     6121 atcggcgctt gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca
     6181 acgattagga atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag
     6241 tgatgagatt tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat
     6301 ttatgatgag ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga
     6361 cggtgtaatg atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga
     6421 tggcgggatg cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat
     6481 cacgacacca gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt
     6541 ccattatgaa atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa
     6601 aaaagctaaa gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat
     6661 gcgtttcaac gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg
     6721 gggacaagaa tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt
     6781 ttattcgtga tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg
     6841 caattcacgc tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg
     6901 atcaattctg tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg
     6961 actgcttatt ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg
     7021 cctacatggg tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac
     7081 ggcagcgtga atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca
     7141 gacttcagtt ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt
     7201 ttcaatgcat tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg
     7261 aatagctttg agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt
     7321 ggtgtccaag cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc
     7381 ggaaatcggg tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac
     7441 tgggggatgg gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc
     7501 ctgacaggcg agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat
     7561 ctttatttag ggatcgtctt tttattctta tatgcaccga tcttctatct gattttttat
     7621 tccttcaatg aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa
     7681 gctgtttttg aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc
     7741 ttatctgcat tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg
     7801 aaaaaacgac gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct
     7861 gatgttatta tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt
     7921 tttcagagtg tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc
     7981 ttgccaaaat tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct
     8041 aataatttcc aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct
     8101 ggatatttta tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca
     8161 ggaaatggct ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt
     8221 ttagaaatca atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga
     8281 tactacttta ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc
     8341 gagggggtgg cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca
     8401 ttattgcggt gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag
     8461 caaaggtact taccatctat aactggggag attatatcga tcctagtttg atcacgaaat
     8521 ttgaaaaaga atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt
     8581 tcacaaaaat ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga
     8641 tccaaaaaat gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat
     8701 taaataatat tgatccacga tttttagact tggattttga tccagataat cagtattcga
     8761 ttccatattt ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc
     8821 aaattaagca ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg
     8881 acggtgcacg agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta
     8941 agaatatggc acagttgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca
     9001 aagcaatcgt ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag
     9061 tgacattttc tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga
     9121 tcccaccaga aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa
     9181 ataaagaagg tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa
     9241 atgcagagta tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag
     9301 acatttcgtc ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct
     9361 acaaagatct aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga
     9421 tgtataggag ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc
     9481 taaaaatcgt cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc
     9541 cgcttttttt gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca
     9601 aaaccattgt aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag
     9661 aaggataaag taattggaaa ctatgcaaat atacttaacc cttctgtaat ctattaataa
     9721 aactaatata tgaatccagt tcaatatttg tagctatgaa aaaacataaa aatcatatta
     9781 gataaaaact aaattggtag ataaattatt ttgtcagata tctgaggcaa atattcgact
     9841 atttgtgttt caaagtaaaa attggaatac aaatatagaa tagacatatt gcagccgata
     9901 gtttattatg cttttagaca cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat
     9961 cgagtttttg atgagctgat acgactaaca gggaatatgt cttctgtcac tgccgagaaa
    10021 atttcgaaga atctaggtat ctctcgtcag aatgccagcc attatttgac gagattagtg
    10081 gaagataaaa aggtcgagaa attaccggga aagcctgttt tgtggaagcc tttagatgaa
    10141 tatgcggttg tagataatac ggagcaaata aacgaagcct ttcattctgt agttggtcat
    10201 gacggaagtc tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac
    10261 ggactcagtg tattaataaa tggagccacg ggtgttggaa aaagtttttt agccacaaaa
    10321 atttttgaat acgctatcca tgagcaaatt attgaaaaag atgcaccatt tgctatctta
    10381 aactgtgccg attatgcgga caatcccgaa ctgctttcgg caacactgtt tggatacaag
    10441 aagggggcct ttacgggggc tgaaaaggat accgatggtt tattggcaac ggccaacaac
    10501 ggctatcttt ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt
    10561 ctatttattg atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct
    10621 aaggttcgtt ttattttcgc cacgacggaa aaaggtgaaa attatttatt agacaccttt
    10681 gatcggcgta ttcaaattag tgttatgttg ccaacttttg aagatcgccc cattcgagaa
    10741 cgattagagc tgattcaatt attttttcaa aatgaagcgg atgttttaca aaaagatatt
    10801 attgtttcaa gagaagcctt gtctttaatg ttatctcatc cctttagtgg gaatattggt
    10861 aagctgaaga atatcattaa aatcagttgt gcggatgtgt atagtcgaac acaagaagaa
    10921 ccattaacga ttgggaaaaa tgagatcttg attcagttaa atatgctgga atccgaagta
    10981 gaaagcttgc cgattgctag tttattgata aagaaaaatc aaaagataaa aacggatcga
    11041 atcaaaccgt ttgattatga ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt
    11101 aatttcaatg aaaatttttc ttcgataaag aatgaagtac agcatttggt gcatcacatt
    11161 gataaggatt cttttttatc aatgagtaga aaattgatga ttcagatgtt tagaaaggtc
    11221 tgggtatccg ttttaggaaa aacgtacgga ttgacgacga gtggacctgt tgcagaaatt
    11281 attagcagat cctatgtcat ttccccaagc tataagacag acttaaccgg gataaatcgt
    11341 acattagctg caaatctacc tcggacgact tacttatgta atcagttctt ggagcattta
    11401 ccaccgctct cagaaaagga cgagaacttt ctaagactgc ttattgtcgt gactttatct
    11461 gattatgtag atgaatccat cgagctgaag ggcctgttgc ttgctcacgg agatagtacg
    11521 gctagcagca ttcaagcagt agtgaatcaa ttatgcggca attatgtttt tgaagcgttg
    11581 gatatgccag tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa
    11641 cggcaagtga atacagatag tctagtgctg atcgtagata tgggctcttt aaatcaaatt
    11701 tacacacaga tcaagaacga tttaaaaggg gagttacttc ttctgaataa tttgacgact
    11761 tccattgcac ttgatattgg cttgaagatg acgatgaatg cgccatttaa acagattgcg
    11821 gaggatgcag cagacaagta ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt
    11881 agcaacatta tcatttcctg tatgtcgggt ttggggattt cggacaaatt gaaagatgtt
    11941 tttcgtcatc atttatcgga tgaatccatc gaagttttca cgaaggacta cagagaacta
    12001 cgtgaattaa tcgaccaaaa tgatgaaact tattttgaca agacaaaatt ggtcatcacg
    12061 acttcggatc ttccacgttc cttttcaatt cctaatatta atgtttatga catcttagac
    12121 ggtgatggag cagccaattt ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt
    12181 aaccgcttga ttcaggagct agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg
    12241 aactttctga acccaacgat cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa
    12301 aattactatc atttcacttt aaatggccgt gtaaaactaa atctctatat gcatacggca
    12361 ttgatgatgg aacgtttatt cttggctcgg acagaaaaat ctgaagaacc gaattatgat
    12421 ttgaaaccag atgaagatga attttattat gtttctagaa gtatttttca atcaatggag
    12481 atgaaatata attttaaagt caatgcttat gagctttcgc tgctctatga attattagga
    12541 ccgtatattc aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt taggttagac
    12601 acttttttta atgtgaatta acgtgattaa accaatgttt ttaaaagttg gcacaggaat
    12661 tgcttatata aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa ttctaatagc
    12721 gactcacggt cacttagctt caggctttct tagcagtatt caattactga cggggaaggt
    12781 tcaagagatt acggtgatta atgcttatgt ggatgagtgt gattttgaac aggagttgaa
    12841 tgtatttgta aaccaggtga acgagcagga tcagattttt gtttttaccg atttatttgg
    12901 cggaagtgtc aatcagaaga ttaccaaaac ctttttggag aaagagattg ctgtaactat
    12961 tttgtctggc tttaatctgc caattctgct cgaaatagta ttggcaacca aggaattaac
    13021 aaaggaggat attcaggaac tggtaaataa atgccaacag gaattaaaga tgaccattct
    13081 tgatgaggta catgtagtaa atgaagagga agattttttt taggaggaag aaaaatgatt
    13141 aaagcagtgc gaattgatga acgattaatt catggacaag tagcgatgac ttggacaaag
    13201 agtctaaatt taaccgggtt agtagtagca agcgatgagg cggctaccaa cgatattcaa
    13261 aagatgacat tgaagatggc tgcaccatcg aatgtcaaag tcattattaa aacagttgat
    13321 gacgcgattc atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat ggttttagtc
    13381 cctaatgtca aagatgcggt aaaagttgcg aaagctttcc caacggaaat cgaattaatg
    13441 aacgtgggga atgctgggaa aatgacaccg ccatcggaag ataagaaaac cttatcaaag
    13501 gaagtgatgt taacgacgtc agaaattcaa gacttaaaag aattagttga actttatcca
    13561 gataccgttt ttcagcctac accggcgatg gaaaaacgtt cagctatctc tattttgagt
    13621 gatttttaga aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc gctttggccg
    13681 tttttattgg aattgctgga catgaaatgt tcgggatgtc catgctgagt cgtccgattg
    13741 ttgttgctcc gttagcagga gcgttgatgg gggatttgca gacggggttg attgtcgggg
    13801 cgtcactaga agccatcttt atgggtgtcg tcaatatcgg aatctctagt acggcggagc
    13861 cttctttggc tgctgggctg gctacagcgt tcgcaatcaa aatgggagga aacgtggatg
    13921 cgattattcc cattgtcttt ccactagcag tattaggatt acagttaatg aatatgattt
    13981 tttcttttgt atgtggaccg atggctgcta aatttttcaa atttgcaaaa tctggtgatc
    14041 aaaaaggaat catcaatctg cacttcttta tgtggtttgt ccactatgcg ctttatgctc
    14101 ttattccatt ctttgccgtt ttatttggag cagatgttgt caaaagcgtc ttggataata
    14161 ttccagaggt catcatgaac ggattaacag tcgctggaaa tctgctgccg gcagttggga
    14221 tggcgatgct gttacggatg ctttggagta aaaatattgc tgtgtggttt ttctttgggg
    14281 ccgttgttat ggcgtatctc gagttaccat tgattgcaat tgccgcagtt ggaacgatta
    14341 ttgcaattgt tatagcccaa agagatctac aactaaaaaa tatatctgtt catgcagttc
    14401 aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga ggaggatttc tttcaatgaa
    14461 attagctgat ggaatttcta aagatgagaa aaaattggtt cggaagatgt tttggcgctc
    14521 gatgactatg tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt tcgcctattc
    14581 tatgatgccc ttcattaatc atttctacaa aagtgaagaa gatcgagctg cggcgctgga
    14641 acgtcataca cagtattttt cgacgaccat tccttgcgcg tcctttgtca tggggattgc
    14701 ggcatcgatg gagaaggaaa attctgaaaa agctggtttt gacgtggaaa gtatcaacgc
    14761 gatcaaaacg agtttgatgg gaccattggc tggaattggc gattcattgt tctggggagt
    14821 ttggcgtgtc gttaccgcgg gactagcgat caacttagcc aatgccggaa atattttggc
    14881 accaattatc tttttagtta tgttcaatat acctaattac ttgatgcgat actatggcgg
    14941 cgttttaggc tattcattag gctctaaata tattgaaaag ctgtatgcga acgggttaat
    15001 tgaaattctg acaaaagctg ctagtatctt gggtctgatc atggtaggtg caatgacgat
    15061 cagtatggtc aaattcaata ccgttttgag cttttcaatg aagggcgaag aactaatgaa
    15121 tgtccaagaa atcctcgata gtatttttaa aggaattgta ccgttattgg taacattgag
    15181 ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa attatttttg gaatggtcgt
    15241 cttgggaatt ttattgtcat tacttggaat cgcatagtag gaagctgcag ctactaggct
    15301 gcagtttttt tagcaaggag ggaaaagatg actattaatg aagtaattta ttcacccaaa
    15361 cacccatttt ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa cgaatacagc
    15421 tactatcgga atattcctga tgtaaaggga ttgaccttcg atgtaagtaa tcaaaagaca
    15481 ttagcaaacc ttgatgtttg cgggacgatt caaacgttga ccttttatcg agaaaattta
    15541 ctaactgagg aaaaaccagg tgtttgggtc aataaacagc tctctcaaac caattcgtta
    15601 tccttaaagg ttgaagtaaa cgggattgtc taccatttgt cagaatcgga tcatcgtatt
    15661 gaagtcgatg tgattcaaga ctgtttgcct agatatattc atcattattc gacgtttaag
    15721 gtcattgtag tatcctttgc tcccatactg gctaataagc gtttaagtat gcttgttcaa
    15781 caggtttaca ttgtgaatga aacacacgaa agccttcaag tttcagtgaa agatgtgcct
    15841 tcgtatcaag agaagtattc ggatcagcaa aatgtgttga tttctcaaaa aggaaataag
    15901 gacacgatcg cacaacagga agtggcaagc ttcgccattg cttttgtgga cccaaatgct
    15961 tttgaagaag taatgacttt tcaggaatct gatatcgaaa aatggctgaa ggaaacattg
    16021 aactattttg ctcagctatt tgggcagctg tcaatgccaa acgatagagt tgtccatctc
    16081 tatcgtcgtg cactttatca aagcttttct agtttcggta tgaatcgaca gggacaggtc
    16141 gtcggcagca attggggcag ctatcctgct actaatcgaa tttggaataa ggatatgtat
    16201 tacgcatcat tgccattcac catgtttgaa ccggagttat gtcaaaaaac ggttctatgg
    16261 tttgatcaat atggcgttaa gtttcctggt tcaaaattta caggaggtat caatcattct
    16321 ctgagtaatt cgttatccag tgcgttactg gctagtcttt attttgaaca tactggggat
    16381 acaaaattct ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat tattgaggac
    16441 atattagctc aacgtcggtc aggggaacca atgctttttc catctgtttg gttatcagac
    16501 gcctttgcac ttggaaagta tcataccggc agcaatcttt gtctttggaa ggcctgtatt
    16561 ggacttgcag aaatttttga agccctagaa caactgagtc ttagcaagcg atacaaaaac
    16621 attgcctgta aactaaagga atcaatttgt aaacaaatga ctatcgatgg tccttttggt
    16681 gagcaatttt tggagggaat cggtgatgag gagaaaacaa cctattcagt gaagcactat
    16741 caaaaaccga ttcttgagca agggttaatc tttttaagtg atgtgattgt ggatggaaaa
    16801 attaatttat tgatgcatga tggagaagaa tcagacacta cgttgatgcc tttttatcaa
    16861 tttttagata ataaagatca tttatatcag aacaccatgg ccttttctgc tagttctttc
    16921 aatccaacct atagcgagga aatcaaaggc atcacttggg gattagaatc cggcgccact
    16981 tttcctggct ttatcactgt tttaatgagc gagctgcaca accctcaagc tttcgtaacc
    17041 cgattagaag aactaatttg tttggcagat ttagacggtt cgtggtggtg gtggccgtat
    17101 cgcttagagg ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa atgcggctgg
    17161 gcatcgggtc tttttgtcag cctaatgatc agtcaatatt tcggattgaa attcaaaaaa
    17221 ggagtattgc aaattgatcc tgttgataat ttaacgtttt cttggaagaa tttgaagttc
    17281 ggtcaagcct tcatttcgat tgaatgtacg caatccgagt tgagtatttc aaatttgtca
    17341 gaaaagccat tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg
    17401 attcacattc aaaggagaaa aagatgagta tactaaaaaa tattacccaa tttaaaaaaa
    17461 tccagagtcg agcaatcact cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt
    17521 tgccaagcga gctaggacca agccgcaaag gtcgtggcgc aattccgctg acttctggtg
    17581 aagaaactgt gattgcggat attactggaa cgggaataat tcgtcatatg tggatgacga
    17641 ttcgtgatcg aacagaattt ggcagttttg ttttacgtga tttaatctta agaatttatt
    17701 gggatgggtc ggatagccct gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt
    17761 ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt taatcctaca gggggcttta
    17821 attcctattt cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag aatttacatc
    17881 cgaaggatat gtcctcattt ttttatacga taaattacgc tttggtggac gaattggaag
    17941 aaaatagcct atattttcac gcaaattggc atcgggaacg cattaccaaa ttacaggagg
    18001 attataagtt aattgatcga ttaaagggtc aaggctatta cgtgggtact taccttgcgc
    18061 ttacctgcct agaacgttat tggtggggcg aaggcgagtt taaattctac attgatgggg
    18121 atagtgagta tccgaccgta acctcaacag gttcagaaga ttattttggc ggggcgtggg
    18181 cctttcatga gtacgatgag ttcggacgtc cacatgcgaa aaactattca acagcctttt
    18241 tagggtatcc ttaccaatca aatagagatg ccacaagaga tcgttttgaa acgggaaaat
    18301 tgaatgccgt tcatgccttt ggtgacgacg caatgccacg acatggtctt tatcgttggc
    18361 atcttcaaga ccccatctgt tttaccgaag atttatcggt cactttacaa caaatcggca
    18421 acgatgatgt tcgcttattt gagcggcaag acgatgttgc tagtgtcgct tactggtatt
    18481 caacagaagc ttcaggaaat actaaacgtc atttcactga tcgagaacgc ttgccgcgtt
    18541 aatgaagtgt ttttcacata ttcgaaggga ggaacctatg atgatatatg gggcaattga
    18601 agctggaggg actaaatttg tctgtgcagt cggtactgag gaattaacga ttttggaaag
    18661 agtcagtttt cccacaacaa ctccagttga aacaatgaag caggtcattg atttttttaa
    18721 accttttaag gaacaattgg caggcatcgg ggtcggctgc tttggtccaa ttgatattca
    18781 atgcaaatca acgagtttcg gtcatatcac atcaacacca aaacttgctt ggcaaaattt
    18841 caattttgta ggaacgctaa agcaatattt caatattccg attgaatgga caacagatgt
    18901 caatgcggct tgctatggtg aatatgttgc tggttctggc aaagggttag caggtgttgc
    18961 atacttcact gtgggaacag gtatcggagg tggtgccttg ttcaatggga ctttcgtgga
    19021 agggttcagt catccggaaa tggggcatat gttgatcaag cgacatccaa aggacaattt
    19081 ttcaggaaac tgtcccttcc accaagattg tttagagggg atggcagcag gaccagcgat
    19141 tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat
    19201 tgaagccttt taccttgccc aatgtgcata caatacaaca ctgatgtttt cgccagatag
    19261 aattattttt ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa
    19321 atttgttgag cttataaatg gatatgttga aataccacca attgatagct acattattac
    19381 gcccgaatta ggtgataatg ccggaataat cggcggatta gcattggcca gaaaagcggt
    19441 aagaaataag caaccataat taatgaatgt ttcattgata ctcatgtcaa aagtatttaa
    19501 ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa aatgaattca aataaaaagg
    19561 ctctaagtca aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc
    19621 ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt cactggtggc gtacacttaa
    19681 atagtaatat attgtgttac tttatgtctg tggtaatagt tatagttgct taccaatgta
    19741 tgttcatttt tcgaaatgtt gacttcataa tccttggctt aatgtttgaa tttttttggt
    19801 gatagatttt ttcagccgta taaaataaat cgtaattcta ttatagagag ttgtgattat
    19861 aaaaagatag caagaaaaaa aaagaaagtt tctatacatt ttaaatattt tattcctatt
    19921 tctattgcta aaaaagtaga aattctatat ttacctttaa ctctcgcttc tcttgcaaat
    19981 gtagttagca ttttaatcga taataaactc aggtagttat attaccttga aatgattgat
    20041 aattttgtca cttctatttt ttttgcaata aattttttat tttagtgaaa aataagaaac
    20101 tatttctttg aaggcgataa gttagaaaaa tattattgta gtcttttctt tattaggaat
    20161 catcactgtt gtatcttatt accttttctg tttttcttat tagagaaaaa taatttgaga
    20221 ggtagatagt ttttattgga ggaagtaatt atgtatattt ttgaaacaaa aatttttcat
    20281 tgcaataatt gcaacaaaga aatttcatcg ggggaagagg tttggacaaa aatggaaatt
    20341 tccgccaaaa gtaactgctc ctcaactgaa atcaattaaa gaactagaat ttgagaatgc
    20401 actggtaatt tgtatgacat gtactgatga agtatttcga aaacattttt aaaggttgag
    20461 atgtatatag atttaaccaa tcatttatct ctctcggaaa aggaatatta actaaccagc
    20521 gggttatcaa aaataggtag gaaaactgta cttaatggct tatttcgcgc tgattatgag
    20581 aatatcagtc tgtttgatta atttttaggg ttcaaaatac agagacaaac tccaggtcgg
    20641 gtttcgccac aagcgaatta cccgatctgg agttttgtct attgcaggaa ggcgtagccg
    20701 gactgcagga ataaagcccc cggtt
//
LOCUS       DACECS010000049        19843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.049, whole genome shotgun sequence.
ACCESSION   DACECS010000049 DACECS010000000
VERSION     DACECS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.049"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWO31_001877"
     CDS             99..293
                     /locus_tag="IWO31_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302286.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWO31_001878"
     CDS             562..861
                     /locus_tag="IWO31_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302287.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWO31_001879"
     CDS             complement(958..1392)
                     /locus_tag="IWO31_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302288.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWO31_001880"
     CDS             complement(1624..2334)
                     /locus_tag="IWO31_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0302289.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWO31_001881"
     CDS             complement(2324..3184)
                     /locus_tag="IWO31_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0302290.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWO31_001882"
     CDS             complement(3189..3827)
                     /locus_tag="IWO31_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0302291.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWO31_001883"
     CDS             complement(3842..4141)
                     /locus_tag="IWO31_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0302292.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWO31_001884"
     CDS             complement(4184..5668)
                     /locus_tag="IWO31_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0302293.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWO31_001885"
     CDS             complement(5688..6149)
                     /locus_tag="IWO31_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0302294.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWO31_001886"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWO31_001886"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0302295.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWO31_001887"
     CDS             7452..8222
                     /locus_tag="IWO31_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0302296.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWO31_001888"
     CDS             complement(8495..9322)
                     /locus_tag="IWO31_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0302297.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IWO31_001889"
     CDS             9890..11125
                     /locus_tag="IWO31_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0302298.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IWO31_001890"
     CDS             complement(12124..12384)
                     /locus_tag="IWO31_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0302299.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IWO31_001891"
     CDS             complement(12411..13712)
                     /locus_tag="IWO31_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0302300.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="IWO31_001892"
     CDS             complement(14004..14462)
                     /locus_tag="IWO31_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0302301.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15800)
                     /locus_tag="IWO31_001893"
     CDS             complement(14475..15800)
                     /locus_tag="IWO31_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0302302.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17229)
                     /locus_tag="IWO31_001894"
     CDS             complement(15817..17229)
                     /locus_tag="IWO31_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0302303.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="IWO31_001895"
     CDS             complement(17246..18010)
                     /locus_tag="IWO31_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0302304.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="IWO31_001896"
     CDS             complement(18114..18989)
                     /locus_tag="IWO31_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0302305.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="IWO31_001897"
     tRNA            complement(19244..19328)
                     /locus_tag="IWO31_001897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="IWO31_001898"
     tRNA            complement(19346..19417)
                     /locus_tag="IWO31_001898"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="IWO31_001899"
     tRNA            complement(19427..19499)
                     /locus_tag="IWO31_001899"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="IWO31_001900"
     tRNA            complement(19561..19645)
                     /locus_tag="IWO31_001900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="IWO31_001901"
     tRNA            complement(19666..19736)
                     /locus_tag="IWO31_001901"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
BASE COUNT         6292 a         4106 c         3096 g         6349 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tgg
//
LOCUS       DACECS010000050        19473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.050, whole genome shotgun sequence.
ACCESSION   DACECS010000050 DACECS010000000
VERSION     DACECS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.050"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWO31_001902"
     CDS             complement(21..995)
                     /locus_tag="IWO31_001902"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0302306.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWO31_001903"
     CDS             complement(1075..1950)
                     /locus_tag="IWO31_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0302307.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWO31_001904"
     CDS             2080..3987
                     /locus_tag="IWO31_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0302308.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWO31_001905"
     CDS             complement(4528..7806)
                     /locus_tag="IWO31_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0302309.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWO31_001906"
     CDS             complement(8077..8622)
                     /locus_tag="IWO31_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0302310.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWO31_001907"
     CDS             complement(8634..9326)
                     /locus_tag="IWO31_001907"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0302311.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWO31_001908"
     CDS             complement(9345..9629)
                     /locus_tag="IWO31_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302312.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWO31_001909"
     CDS             complement(9642..10205)
                     /locus_tag="IWO31_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0302313.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWO31_001910"
     CDS             10433..11095
                     /locus_tag="IWO31_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0302314.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWO31_001911"
     CDS             11219..12415
                     /locus_tag="IWO31_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0302315.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWO31_001912"
     CDS             complement(12516..13892)
                     /locus_tag="IWO31_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0302316.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWO31_001913"
     CDS             complement(13929..14795)
                     /locus_tag="IWO31_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0302317.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="IWO31_001914"
     CDS             complement(14884..15909)
                     /locus_tag="IWO31_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0302318.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWO31_001915"
     CDS             complement(15927..16601)
                     /locus_tag="IWO31_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302319.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWO31_001916"
     CDS             complement(16785..17678)
                     /locus_tag="IWO31_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0302320.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWO31_001917"
     CDS             complement(17752..19449)
                     /locus_tag="IWO31_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0302321.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6155 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt tta
//
LOCUS       DACECS010000051        19457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.051, whole genome shotgun sequence.
ACCESSION   DACECS010000051 DACECS010000000
VERSION     DACECS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.051"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWO31_001918"
     CDS             133..3660
                     /locus_tag="IWO31_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0302322.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWO31_001919"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWO31_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0302323.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWO31_001920"
     CDS             7448..7789
                     /locus_tag="IWO31_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0302324.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWO31_001921"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWO31_001921"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0302325.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWO31_001922"
     CDS             9146..11566
                     /locus_tag="IWO31_001922"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0302326.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO31_001923"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO31_001923"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0302327.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWO31_001924"
     CDS             13000..13737
                     /locus_tag="IWO31_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302328.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWO31_001925"
     CDS             13751..14566
                     /locus_tag="IWO31_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0302329.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWO31_001926"
     CDS             14579..15226
                     /locus_tag="IWO31_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0302330.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWO31_001927"
     CDS             15242..15898
                     /locus_tag="IWO31_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0302331.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWO31_001928"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWO31_001928"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0302332.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWO31_001929"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWO31_001929"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0302333.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWO31_001930"
     CDS             18240..18758
                     /locus_tag="IWO31_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0302334.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWO31_001931"
     CDS             18865..19365
                     /locus_tag="IWO31_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0302335.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DACECS010000052        19165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.052, whole genome shotgun sequence.
ACCESSION   DACECS010000052 DACECS010000000
VERSION     DACECS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.052"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1152
                     /locus_tag="IWO31_001932"
     CDS             <1..1152
                     /locus_tag="IWO31_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302336.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IWO31_001933"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IWO31_001933"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0302337.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IWO31_001934"
     CDS             2728..3003
                     /locus_tag="IWO31_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0302338.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IWO31_001935"
     CDS             3009..3776
                     /locus_tag="IWO31_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0302339.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IWO31_001936"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IWO31_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0302340.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IWO31_001937"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IWO31_001937"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0302341.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IWO31_001938"
     CDS             7920..8222
                     /locus_tag="IWO31_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0302342.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IWO31_001939"
     CDS             8225..9388
                     /locus_tag="IWO31_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0302343.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IWO31_001940"
     CDS             9414..12842
                     /locus_tag="IWO31_001940"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0302344.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IWO31_001941"
     CDS             12863..13987
                     /locus_tag="IWO31_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302345.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IWO31_001942"
     CDS             13977..14414
                     /locus_tag="IWO31_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0302346.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IWO31_001943"
     CDS             14398..14736
                     /locus_tag="IWO31_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0302347.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IWO31_001944"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IWO31_001944"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0302348.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IWO31_001945"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IWO31_001945"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0302349.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IWO31_001946"
     CDS             15893..16936
                     /locus_tag="IWO31_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0302350.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IWO31_001947"
     CDS             17095..17823
                     /locus_tag="IWO31_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0302351.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IWO31_001948"
     CDS             17939..18184
                     /locus_tag="IWO31_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0302352.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IWO31_001949"
     CDS             18181..18399
                     /locus_tag="IWO31_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0302353.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IWO31_001950"
     CDS             18535..19074
                     /locus_tag="IWO31_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0302354.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
BASE COUNT         6507 a         3309 c         4209 g         5140 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgggctctt tgtcaaatag
    19141 gactgataga gtgagataag aacat
//
LOCUS       DACECS010000053        19126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.053, whole genome shotgun sequence.
ACCESSION   DACECS010000053 DACECS010000000
VERSION     DACECS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.053"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWO31_001951"
     CDS             complement(<1..703)
                     /locus_tag="IWO31_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302355.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWO31_001952"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWO31_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWO31_001953"
     CDS             1758..2837
                     /locus_tag="IWO31_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0302356.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWO31_001954"
     CDS             2869..3462
                     /locus_tag="IWO31_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302357.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWO31_001955"
     CDS             complement(3572..3799)
                     /locus_tag="IWO31_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0302358.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWO31_001956"
     CDS             complement(3902..6490)
                     /locus_tag="IWO31_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0302359.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWO31_001957"
     CDS             complement(6510..7169)
                     /locus_tag="IWO31_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0302360.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWO31_001958"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWO31_001958"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0302361.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWO31_001959"
     CDS             complement(7942..8808)
                     /locus_tag="IWO31_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0302362.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWO31_001960"
     CDS             8917..9558
                     /locus_tag="IWO31_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0302363.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWO31_001961"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWO31_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0302364.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWO31_001962"
     CDS             complement(10999..11898)
                     /locus_tag="IWO31_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0302365.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWO31_001963"
     CDS             complement(11900..12697)
                     /locus_tag="IWO31_001963"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0302366.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWO31_001964"
     CDS             complement(12675..13379)
                     /locus_tag="IWO31_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0302367.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWO31_001965"
     CDS             13510..14085
                     /locus_tag="IWO31_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0302368.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWO31_001966"
     CDS             14203..14880
                     /locus_tag="IWO31_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0302369.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWO31_001967"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWO31_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0302370.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWO31_001968"
     CDS             complement(16943..17941)
                     /locus_tag="IWO31_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0302371.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWO31_001969"
     CDS             complement(18167..18820)
                     /locus_tag="IWO31_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0302372.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DACECS010000054        18931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.054, whole genome shotgun sequence.
ACCESSION   DACECS010000054 DACECS010000000
VERSION     DACECS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.054"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            163..2634
                     /locus_tag="IWO31_001970"
     CDS             163..2634
                     /locus_tag="IWO31_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0302373.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2737..4473)
                     /locus_tag="IWO31_001971"
     CDS             complement(2737..4473)
                     /locus_tag="IWO31_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0302374.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4470..5174)
                     /locus_tag="IWO31_001972"
     CDS             complement(4470..5174)
                     /locus_tag="IWO31_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302375.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5305..5808
                     /locus_tag="IWO31_001973"
     CDS             5305..5808
                     /locus_tag="IWO31_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0302376.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5768..6106
                     /locus_tag="IWO31_001974"
     CDS             5768..6106
                     /locus_tag="IWO31_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0302377.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6127..7545
                     /gene="ffh"
                     /locus_tag="IWO31_001975"
     CDS             6127..7545
                     /gene="ffh"
                     /locus_tag="IWO31_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0302378.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7657..8235
                     /locus_tag="IWO31_001976"
     CDS             7657..8235
                     /locus_tag="IWO31_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0302379.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8239..8760
                     /locus_tag="IWO31_001977"
     CDS             8239..8760
                     /locus_tag="IWO31_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0302380.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8839..9393)
                     /locus_tag="IWO31_001978"
     CDS             complement(8839..9393)
                     /locus_tag="IWO31_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0302381.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9646..9921
                     /gene="rpsP"
                     /locus_tag="IWO31_001979"
     CDS             9646..9921
                     /gene="rpsP"
                     /locus_tag="IWO31_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0302382.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9933..10184
                     /locus_tag="IWO31_001980"
     CDS             9933..10184
                     /locus_tag="IWO31_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0302383.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10309..11100
                     /locus_tag="IWO31_001981"
     CDS             10309..11100
                     /locus_tag="IWO31_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302384.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11270..11791
                     /gene="rimM"
                     /locus_tag="IWO31_001982"
     CDS             11270..11791
                     /gene="rimM"
                     /locus_tag="IWO31_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0302385.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11781..12542
                     /gene="trmD"
                     /locus_tag="IWO31_001983"
     CDS             11781..12542
                     /gene="trmD"
                     /locus_tag="IWO31_001983"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0302386.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12678..13025
                     /gene="rplS"
                     /locus_tag="IWO31_001984"
     CDS             12678..13025
                     /gene="rplS"
                     /locus_tag="IWO31_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0302387.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13157..14293)
                     /locus_tag="IWO31_001985"
     CDS             complement(13157..14293)
                     /locus_tag="IWO31_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0302388.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14409..15323)
                     /locus_tag="IWO31_001986"
     CDS             complement(14409..15323)
                     /locus_tag="IWO31_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0302389.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15414..15842)
                     /locus_tag="IWO31_001987"
     CDS             complement(15414..15842)
                     /locus_tag="IWO31_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0302390.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15847..16221)
                     /locus_tag="IWO31_001988"
     CDS             complement(15847..16221)
                     /locus_tag="IWO31_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0302391.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16507..16692
                     /locus_tag="IWO31_001989"
     CDS             16507..16692
                     /locus_tag="IWO31_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0302392.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16704..16952
                     /locus_tag="IWO31_001990"
     CDS             16704..16952
                     /locus_tag="IWO31_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302393.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            16983..17162
                     /locus_tag="IWO31_001991"
     CDS             16983..17162
                     /locus_tag="IWO31_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302394.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17205..17675)
                     /locus_tag="IWO31_001992"
     CDS             complement(17205..17675)
                     /locus_tag="IWO31_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302395.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17762..18460
                     /locus_tag="IWO31_001993"
     CDS             17762..18460
                     /locus_tag="IWO31_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0302396.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18638..>18931
                     /locus_tag="IWO31_001994"
     CDS             18638..>18931
                     /locus_tag="IWO31_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302397.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT         6223 a         3320 c         3719 g         5669 t
ORIGIN      
        1 tttccaccaa cacaaaatat tatagaaccc tttttttttg ctatatgaca ggaatgaaag
       61 gtgtttgcaa gcttaatgtg aggaacactt tccatatctg ttctatactg gataggagaa
      121 gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa taaatggata
      181 aatcgaagta aaaaaaagaa agtaacacaa agatcatcag ataaagtcat ccgtcttttt
      241 ccatcgtata aagaaggatt aacggcagat caagtcaagg aacgaataga aaaaggtgca
      301 gcaaataatt cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt ggaaaacata
      361 tttacgtatt tcaatttgat ctttcttatc ttagccattt tgctttgttt agtagaatcc
      421 tataagaact tgactttttt accagttatc atagccaata caggaatcgc catttttcag
      481 gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt taaatacagc aaaagtcaat
      541 gtcatacgag acgggataga acaaacaata gatatagaag accttgtaat agatgacatt
      601 gttattttag agactggata tcaaattcca gcagatgcag aggtcgtgga agggaaactg
      661 caagttaatg aggctttatt gacaggcgaa gcagatgaaa taaccaaaga aataggtgat
      721 ccattaatgt ccggcagttt cattgtttcc ggaaaagctt acgcccgatt agacaaagtt
      781 ggagcagatt cgtatatttc ccagctgaca gtcaaagcca agacaatggg agaaggagaa
      841 cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat ggattgggat catcattatt
      901 cctatcggta tcctgctttt ttctcaaagt tactttttta atggaaacac gatcaaagaa
      961 agtgtcgttg caatggaagc agcattgatc ggtatgatac ctgaaggact gtacttgctg
     1021 acgacgattg cattagcatt aagtgcgact aaactagcaa agcaaagagt tcttttacac
     1081 aatatgaaaa gcattgagac attggcaagg gtaaatgtcc tttgtgtaga taaaacagga
     1141 acgatcacag aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa acaagaaata
     1201 atcagtcagt cagatcaatt ggaagcatgc attgctgact atgcaaaagc gatggcagga
     1261 gacaatgcaa caatggaagc tgttaaagca tattttacaa aaaccactga tcaatcctat
     1321 caccatgtta ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag
     1381 gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa
     1441 gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg
     1501 attcttgaag aaccagtatt gacagaagca gtcacaccat taggctatat tctgattgct
     1561 aatcctatca gaaaagaagc aagagccaca tttgattact tcaaaaaaca gcaagtagcc
     1621 atcaaagtga tttctggaga taatccgctc accgtatcca atgtagctgt ccaagcaggg
     1681 atttcacatg ctgatcagta cgttgatgca tctaaattat cagaagaaga atatgaagca
     1741 gctgtcgaaa aatatacagt ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt
     1801 caattgataa agcgtaacaa tacagtagct atgacaggtg atggcgtgaa tgatattcta
     1861 gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc cgccatgcag
     1921 gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt tgttgcagaa
     1981 ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt gaagaatatt
     2041 ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc tttagaacca
     2101 tcacaaatca cattgattag tttgtttact atcggacttc catctttctt gcttgctcta
     2161 gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga aaaagctgtg
     2221 ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg
     2281 aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc agtagggttc
     2341 ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat cattcttttc
     2401 tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc aatcaccggt
     2461 atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga ttcgattttt
     2521 aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa agagcaaaag
     2581 ccgtttagtc gcgtagctaa aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa
     2641 gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag caggatgagt
     2701 catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa tggtaacggt
     2761 gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccattaa ggagttgcac
     2821 gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt
     2881 gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga tacctatacc
     2941 aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa taatctcgct
     3001 gtcgcttttt gaatattgga cagcattttc aattagattt ttgataattg gataaaggat
     3061 atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca accgtttctt
     3121 ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg tataaattgt
     3181 ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc gaatcagttt
     3241 atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc
     3301 atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca caggagtttt
     3361 taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac gtcgcacttt
     3421 cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag taaagtagcg
     3481 caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat ggatcagcgg
     3541 atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa tgacttctgt
     3601 gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct ttggattaag
     3661 cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca gttcgttcag
     3721 taacgtatat aattgctctt ctgtggtagt acacgcccga tacatcttac tcatttgttc
     3781 gctcaacgca ttaatcgtct gatacaattc ttcccattga tcgggtgttt gcatgattac
     3841 ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg
     3901 ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga agaaagacag
     3961 aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg
     4021 ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta atgcgacata
     4081 taaaagatcc tcattcaaag tagtactttt gcgcaaagcg ctacctaggt tcccaccatt
     4141 caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt cattgtttgt
     4201 gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg actgatggac
     4261 gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat ctaattgccg
     4321 gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga tctgctgttg
     4381 aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa
     4441 gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa cttatagcca
     4501 aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat tttttctcgc
     4561 aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg
     4621 cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt
     4681 aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac ttgataattg
     4741 gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc atcttttgat
     4801 tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac ttcgcgagga
     4861 ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat ccgatccact
     4921 tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg acgcagccgc
     4981 tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat gaaatcaaat
     5041 gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt taccttgtag
     5101 ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc atcaacaact
     5161 agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata ttttagcata
     5221 gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga aggcactttg
     5281 ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg cggtctggcc
     5341 tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt tgaaactgga
     5401 gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag agatcccgct
     5461 gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt atggacgaca
     5521 agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg
     5581 caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg cggaacccat
     5641 tcgatttatg acggaagttt ttccggaata aaaaaaaatg gtgccggagt ggctactgct
     5701 tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa aactagaggt
     5761 gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat tttattcgac
     5821 cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg atttttcatt
     5881 aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca atatcaagcg
     5941 gacaagcaag atcttggagg attatgaaaa aaaactccat cttttttcta attatattgt
     6001 tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc cggaagataa
     6061 aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa aaggaatgaa
     6121 gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg aaaaataaga
     6181 aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat ccgccttgcc
     6241 ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa
     6301 cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc
     6361 gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa taaatcacca
     6421 aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac gacctttact
     6481 ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg
     6541 gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt
     6601 cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca agggatggag
     6661 ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg tctgcatatc
     6721 gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt
     6781 ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga tagcttcaac
     6841 cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac tcgaggcggg
     6901 gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg ttctggtgaa
     6961 aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat ccttggtatg
     7021 ggggatatgc tgacattgat tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa
     7081 gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat tgaacagctt
     7141 gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc aggaatgaat
     7201 caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg
     7261 atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa cccaagccgt
     7321 cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa
     7381 caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat gaacatccca
     7441 ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat
     7501 cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga aaaaaacagc
     7561 ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt tttattcttt
     7621 actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa
     7681 acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata tgatgaccaa
     7741 aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg gcagacgatt
     7801 ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca
     7861 aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat tcgtaaccga
     7921 agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc
     7981 ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga ttcttcttat
     8041 cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc aaaagatcta
     8101 aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt agaagctgtc
     8161 gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc
     8221 agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag tttagatggc
     8281 tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac aggtaagcaa
     8341 actacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag aaaaacttat
     8401 caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt
     8461 ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga agatcctgtt
     8521 gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt gggtggcggt
     8581 cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat ccagattacc
     8641 cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga agcgcgtttt
     8701 gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt acgaaagtag
     8761 aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata gttgcctttt
     8821 ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat acgcatctaa
     8881 aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat caattagatt
     8941 cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca aaaagacaag
     9001 tgattggcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac
     9061 gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc ctacatctaa
     9121 agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga aaacgacacc
     9181 gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct gttctggagt
     9241 ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac tttcgtaccc
     9301 ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat cttttcttaa
     9361 actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc aaactatcta
     9421 taataatctt acaaaagata agtaactgga gcaaataaaa acacttactg acaaaagcta
     9481 catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc tattttctaa
     9541 aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg
     9601 ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc agttaaaatc
     9661 cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt agctgattca
     9721 cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc tttgaaagac
     9781 cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa aggtgcacaa
     9841 ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa acaccacgaa
     9901 gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa
     9961 caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc gtagagtcga
    10021 aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt atcattggaa
    10081 aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg atcaatgcac
    10141 cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac gtgctgtact
    10201 cgaattttcg agtgcagctt ttttgttttt tttattaatt gaagaaatga ttatttggca
    10261 aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat gagaaagaaa
    10321 atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac tcaagaatca
    10381 gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga aaaaagtagt
    10441 accaaaacgg cttccaccac tgaacaaaca atggctacct caaataatga gaaaaagact
    10501 gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga tgtaccagtc
    10561 tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc tattttatat
    10621 tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga aacgcctatt
    10681 gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca agttttgcag
    10741 ccatttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat cacagggtat
    10801 cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg gcgtattcga
    10861 atccgtggga ataatataga aggccaagat cccttactat tagctaaaga aatcgtagct
    10921 tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac tgttgatatg
    10981 ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa tgcctatacg
    11041 atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa gaggttgtga
    11101 aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt aatcaaaaat
    11161 agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact agaattgcct
    11221 cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt tgactgaata
    11281 cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc
    11341 aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgctaacac tattccaaga
    11401 cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa attttgactt
    11461 attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg atggcatatt
    11521 aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt accatgagat
    11581 catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag aaatcttgtc
    11641 accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata tattgcttcc
    11701 atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc gtgtagaaat
    11761 tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca agaatgtttg
    11821 aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta ttagatatca
    11881 atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat gattatccat
    11941 acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac atccaagcca
    12001 ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac
    12061 gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta ttcatttgtg
    12121 gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa gtttccttag
    12181 gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat gcaacagttc
    12241 ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct tattcaaccg
    12301 gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg aaagtaccag
    12361 aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa gaatcactaa
    12421 gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca cctcaaatga
    12481 cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat gaagccaagt
    12541 gactttttct aaaatcttct ttacagtagg ttgataatat gttagtattt tatggtgagt
    12601 gcaaaagcac ttaactatta cgatattccg ctgtgagaaa agctcataag aatatttgga
    12661 ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa caattacgtt
    12721 ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag
    12781 gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc ggtgctggaa
    12841 tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc
    12901 cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg
    12961 caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc
    13021 gttaatcatc gttatcagac tgagaaagaa cccttggtat tcaagggttc tttttttgtt
    13081 ctctgttttt ttatattaac gatttctatt tgggacattt ttgattaata aaaaggggca
    13141 aaaaaggggc aacaagttac agtttttcta tatcttcttt caatttttct tttactccct
    13201 ttgtcacatg caaataaatc ttttcagtga tatcactatt ttcgtgccca acgcgatcct
    13261 gaatagcata cagaggagtt cctagttccg ctaattttga aatatgggta tgtctaaaaa
    13321 tatgtgaact aagtttttta tcaatcttca tgtcagcttt atgatttctc aaataggtgt
    13381 taattgctgt tagttggaaa ggagttcctt tcgttgtttg aaatagaaat tgaccattcg
    13441 gatttaattc tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa tcaatttctc
    13501 gcattccagc agcagttttt gtagaatcag attttttcat atcagctatt gaacgttctc
    13561 tatacatcat cgtcccattt ataactactg atgcattatt attggtaatg tgtacatcgt
    13621 ctttacttaa tgcgattgct tctccaggcc tcattccagt caaatatagc cactggaaaa
    13681 ggagagaata tcttttattg tgtgaagttg tgaaatttac tagtctttta tattcatcat
    13741 cttctaaaaa cttatcttta atttttatcg tttttgactc tcgtttataa tcaataacta
    13801 cttcgttgat aggatttttt tctacatagc cttttttcat agcgtatgaa aaaagaagat
    13861 tcaatttgga tttaattaca ctgacgtact tatttgacaa gtcatcttta tatatcatat
    13921 cttcaaaaat attatttaaa tctactgtat taataccaga aacgatgtaa gtctcaggta
    13981 tctttttctt tatagtgttt aagatattat tcgtaggata atatgtagac tccttaactt
    14041 gtcttttgta aataactaac cattcttcga caagctcatg aaatgttaga tcaggcttct
    14101 gaagtgtttt ttcgtttaat tttatatcaa tttttttatt taactctagc aatgccactt
    14161 tctgagtttc tcgagattta tttttataag taatgctcac tttttttctt tttctagttt
    14221 ttggatcaat atatcgctca ttatatttat atactttttt tccgttttta tcagttttgg
    14281 tttcaatcca cattgttttc atctcctatt tgttgctata ataggaatgg ataagtaagc
    14341 caattatagc aggtttattt ttcatcacgt ccacaaactt tggcgaggga gggggcgtgt
    14401 ttttttgttt agtagctacc tttccatgaa gtatctgcga agaacacttc aaatgttgtt
    14461 ccatcatttt tcacttcaaa atatgctgtt ccttgtgcgg atttacccgg ttggatggtt
    14521 tccgaaaagt aatctctact agctaaatca gatttaatat ctgatgaatc ataaaattct
    14581 aaataacttg aacttgcatc aaaaggtgta gtccctgtgt tttctacaag aaaatctacc
    14641 tttgcgtaaa ataggccttc tggtttatag aaatcatcac cgtaatcttt tgatacagac
    14701 tggacagtta cgttaataga gttaccattt tcagaagtaa aaggaactgc ttctcctata
    14761 ctatgtgcaa ctgatgccgt ctggggttct gaagacgaag ttgtagttgt agttgtagta
    14821 gagcttgaat ctttggattt tccatcttta tctaagaaag ttcgttgatt ataatataca
    14881 acctgttcag tttttggact aacccaaatt aacattgttt catcatcaga atcagttgaa
    14941 taagtatatt ggagcatctc taattttttt atatcttttg cactatttcc atgttcaatt
    15001 aattcagagt gctctttata ataatctcct tgtgcatcct ttgaaataga tacaattaat
    15061 tcatcccgtg tgatagcatt gtctaattct ttgtaaacaa aatcatttgt ccatttacgt
    15121 aacggtgaac caagtgtctg ataaacttga gttaaatcat caccaacttt tattttatga
    15181 acgtctgatg aagttagttt ttttgtttca tattcagatg ttgatgaaac agtattttct
    15241 ttagtgctag cagaattagg actagtggag catccgctta ataccaagcc taaaaaaagc
    15301 aacccaaaaa caattttttt catttttaat tcctcacttc tatgatatga tttttttgta
    15361 ggatcttaga aataaggttt ttagtccgtg ttgcagcacg gacttttttt acattatata
    15421 agaaagcgat aagcgctttc tggaagtccg taaagattct ttaattcctc aattcgttta
    15481 ggatattgat cattgtcttc tttgtataga gaaacaatga gattagcagc aaagcaatta
    15541 gcttcgcttt cagatttgct tctagatgtt cttgttgata catagtaact ggataagcca
    15601 cgatgaaaaa tagcgtgacc taattcgtga gcgcaaatgt agaatctttc ctcagagtct
    15661 ctcagttcat catttaagaa gattattgca cgacctctaa tttcttgaaa ctgtcctttg
    15721 ggattctcga taaaaggaac gtattgaatt ttaatgccca tcttttcaca aatataaaaa
    15781 ggattagcgg actggtattt ccgcttcaac tcctcgacta aattaatcgt atccatctcc
    15841 ataagctcac atctttttgc ctttttcttt gtcttctttc acaatatccc agaaggttcc
    15901 aataaggata tcttttacgc gttggacctg ttcaggcgtc agtgtttctc cgccataaga
    15961 catattgaca tttgaatcta gtaatttatc aagttcaact acttcatctt ttgttgccca
    16021 gtcaggaact tggtttctac cgagtaaata atcagtagtt acattaaaat aatctgctac
    16081 aagttgtagt cttttagtac taggagttct atttttccat tgataaatcg tgtttttagg
    16141 tatatttaat tcttcttcca attgagttat acttatgcct cttttatgag ctaattcctt
    16201 tattctatct agtaaattca ttttcgcacc tcacaagcta cgaaaacact aataaaaaaa
    16261 ttagttaaag gtgttgacaa ctaataaaag tgttagtata ctgttttcgt aagctaaaat
    16321 attagctaaa aagacttgat aactaataaa cacttcacgg tcggcaaact ctgaaatgta
    16381 aattactagg catttctgtg tcttatttag ctatgtctat atactaataa aaatattagt
    16441 tattgtcaac gaatattagc taatttttta gcttacagat tattttttta gaaaggagct
    16501 atttttatgt ctgagaattt agacttaaaa attcgagcgg agatgagaaa aagaagaatg
    16561 actttcaaag aactagctgc gcttatcggt atttcaggag cttatttatc agatattcta
    16621 aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa aaaaatattg
    16681 gacatccgat aggggggtag gcgatgacaa aactaaaaaa acaagatttt gtaaaaaaat
    16741 acaattattc tccatctact tatcaacgtc gaatgtcgga actaaaaaat acagcaattt
    16801 tctcagcggc gtatgaacgg gtcacaggac aagaagtttg gatcaataca gaattatacg
    16861 ataaattttt gtctttcaaa tcctataaca ggttacgcac aagaaaggta acacctaaag
    16921 aatttatcga gaagcattta gttgatttat aaaaaacaga acattttgag aggtgaaggt
    16981 taatgggtaa attcaacaga gcattggtat tcagcgcacc gctaatcatc tacgctttag
    17041 gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt tggattttta
    17101 tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag ggggctgact
    17161 aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt taattctttc
    17221 actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg gatttgggtt
    17281 ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa attaattttc
    17341 cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt tagcggtaca
    17401 gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa tttcttaaat
    17461 ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt aactaacata
    17521 gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc tgcagcgaag
    17581 taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt tatccctgtt
    17641 aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt taagtgaaaa
    17701 aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga gagaagaaat
    17761 aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct tacaagtggc
    17821 agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac taaaaaaaga
    17881 tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat atggcagaaa
    17941 tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg ggtttacagg
    18001 aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa tggaagatgt
    18061 tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc aattagaaga
    18121 aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa ttagcggaca
    18181 acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa
    18241 agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt
    18301 taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat tcgatgatgc
    18361 tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa ttgatcaact
    18421 gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg ctaagaatag
    18481 ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta agccaacaac
    18541 atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg caagcaaaga
    18601 gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac tttccactct
    18661 agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa caagtggtcg
    18721 aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag atgatttagg
    18781 caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc attcgaataa
    18841 acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag agtacgacag
    18901 agataacttg attgatatct cttattacgg a
//
LOCUS       DACECS010000055        18739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.055, whole genome shotgun sequence.
ACCESSION   DACECS010000055 DACECS010000000
VERSION     DACECS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.055"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            255..530
                     /locus_tag="IWO31_001995"
     CDS             255..530
                     /locus_tag="IWO31_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302398.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2615
                     /locus_tag="IWO31_001996"
     CDS             543..2615
                     /locus_tag="IWO31_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0302399.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2780..4666
                     /locus_tag="IWO31_001997"
     CDS             2780..4666
                     /locus_tag="IWO31_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0302400.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4678..5625
                     /locus_tag="IWO31_001998"
     CDS             4678..5625
                     /locus_tag="IWO31_001998"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0302401.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5645..6172
                     /locus_tag="IWO31_001999"
     CDS             5645..6172
                     /locus_tag="IWO31_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0302402.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6235..6888)
                     /locus_tag="IWO31_002000"
     CDS             complement(6235..6888)
                     /locus_tag="IWO31_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0302403.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7020..7862
                     /locus_tag="IWO31_002001"
     CDS             7020..7862
                     /locus_tag="IWO31_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0302404.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8020..8916
                     /locus_tag="IWO31_002002"
     CDS             8020..8916
                     /locus_tag="IWO31_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0302405.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8919..9632
                     /locus_tag="IWO31_002003"
     CDS             8919..9632
                     /locus_tag="IWO31_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0302406.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9652..10170
                     /gene="msrA"
                     /locus_tag="IWO31_002004"
     CDS             9652..10170
                     /gene="msrA"
                     /locus_tag="IWO31_002004"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0302407.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10167..10394
                     /locus_tag="IWO31_002005"
     CDS             10167..10394
                     /locus_tag="IWO31_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0302408.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10546..11097
                     /gene="lepB"
                     /locus_tag="IWO31_002006"
     CDS             10546..11097
                     /gene="lepB"
                     /locus_tag="IWO31_002006"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0302409.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11180..11722)
                     /locus_tag="IWO31_002007"
     CDS             complement(11180..11722)
                     /locus_tag="IWO31_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0302410.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11959..12357)
                     /locus_tag="IWO31_002008"
     CDS             complement(11959..12357)
                     /locus_tag="IWO31_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0302411.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12617..13477
                     /gene="ylqF"
                     /locus_tag="IWO31_002009"
     CDS             12617..13477
                     /gene="ylqF"
                     /locus_tag="IWO31_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0302412.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13470..14237
                     /locus_tag="IWO31_002010"
     CDS             13470..14237
                     /locus_tag="IWO31_002010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0302413.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14293..15150
                     /gene="dprA"
                     /locus_tag="IWO31_002011"
     CDS             14293..15150
                     /gene="dprA"
                     /locus_tag="IWO31_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0302414.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15272..17350
                     /gene="topA"
                     /locus_tag="IWO31_002012"
     CDS             15272..17350
                     /gene="topA"
                     /locus_tag="IWO31_002012"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0302415.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17319..18695
                     /gene="trmFO"
                     /locus_tag="IWO31_002013"
     CDS             17319..18695
                     /gene="trmFO"
                     /locus_tag="IWO31_002013"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0302416.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5091 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaacgagtcc aataaaataa aaaggtaaaa attttcaagt
     2641 tttataaaaa acaaaacata tcagaacagg atcgaatcgg tcctgttctt tttttgagaa
     2701 cacaaaaaga acaaatacca aaaaaatttt cctgaagttc actattttta tgatacaata
     2761 tccaagataa ggagggaaca tgttgaaaga attaaaagta actgacctca aaaaaactta
     2821 tggtgaaaaa gatttgtttg accagatttc ttttttggtc cacgaaaaag accgtatcgg
     2881 gttgattggg acgaacggga cgggtaaaac aagtctctta aatattattg ccggtatcga
     2941 tagcagcgac ggtgatcggc aaacagtgtt ttatccaact gattaccgta ttggttatct
     3001 ttctcaagca gctgaatttt ccgatgaatt aactgttctc caagcagtct ttcaaggaaa
     3061 cagccccttg atccaaactg ttcgagcata tgaggaagca ttgatcgaac taggggaaaa
     3121 cggagaagac cctgatgtgc aaaaacgtta tgctaaagca gaagaacaaa tgaataaaga
     3181 agatgcttgg actacagata ccaacgcaaa gattattttg caaaaattag gaatcagtga
     3241 attagataaa aaaatcagta ctttatcagg tggtcagaaa aaacgagtta gtctggcgca
     3301 agtgttgatt gatgaacccg atttactatt actagacgaa ccaacaaacc atttagatta
     3361 tgaagcgatc gagtggttgg aaaactattt gaaacaatac cgtggcgcac tattgatggt
     3421 tacccacgat cgttattttc tagatagagt agcaaaccga atcttcgaac tttcatttgg
     3481 aaaattatac gagtataaag gaaattatga agcttacgtc ttagaaaaag cagaacgtga
     3541 tcgtgtagaa gtagaacaag aagaaaagcg caagcggctt tataaacagg aattagcttg
     3601 gatgagggct ggcgtacagg cacgtggcac caagcagcaa gcacggatca accgtttcga
     3661 agatctaaag gagaacttgt accaagtaaa ccaagaagat gatttagagc tgaacctagc
     3721 gacacaacgt ttagggaaaa aagtactgga aatcaaagat ggcagttacc gtataaatga
     3781 ccaaacactg ctagaacatt tagatttatt gattcaatct cgggaaagac taggtattac
     3841 tggtaaaaat ggtgcgggaa aatcgacatt gcttaatatt ctagctggca gaattcccct
     3901 ggatagcggt acaatgtcta tcggtgaaac ggttcatctt gcgtattata cacaagaaaa
     3961 tgaagaaatg gcaccagata aacggatgat tgcttactta caagaagctg ctgaagaagc
     4021 aaaaacagca gatggcagtc aaatcggagt agcagaatta cttgaacgat ttttatttcc
     4081 aagatttatg catgggacac tgataggcaa actttcaggc ggagaaaaac gccggttatt
     4141 tttgttgaaa ctgctgattc agcaacctaa tgttttatta ttggatgaac cgaccaatga
     4201 tttggacata gcaacattga cgattttgga agattatttt agaagttttc caggtgctgt
     4261 gatcactgtt tcccatgacc gttatttctt ggacaaggta gcagataagt tattagtatt
     4321 ccaaggaaac ggaaaacaag aactttatta cggaaacatg tccagttatt tactgaagca
     4381 aaaagagacc cagcagcctg ctgaaaaagc gaaaccaaaa atacagagca aggaacctgc
     4441 tgaaaagaag aaattatctt acatggaaca aaaagaatgg gaaacgattg aagatgagat
     4501 tgcagaactt gaagaaaaaa tctcattgct ccaagaagag atgaatcatc aaggagataa
     4561 tttcactcga ttacaagaat tgcaaaacga cgtatctgaa acagaagcac aacttgaaga
     4621 aaaaatggca cggtgggaat acttgagtga atgggtagaa gattaggagg aaaaaggatg
     4681 gaacaagcgt atcttgactt aggaagaaaa ttgttggaag aagggcatcg taaagaagac
     4741 agaacaggca caggcacatt aagcttattc ggataccaaa tgcggtttga tctgcaaaaa
     4801 gggtttcctt tgttaacaac gaaacgagta ccttttggat tgatcaaaag cgaattgctt
     4861 tggtttttaa aaggagatac gaatatccgc tatctattac aaaataaaaa ccatatttgg
     4921 gatgaatggg catttgaacg ctacatcaaa agtccagact ataatggacc agatatgact
     4981 gattttggcc atagatgttt gacagatcct gaattcaatg aagaatataa aaagcagagc
     5041 aagctttttt gcgagaagat cctaaccgat gattcctttg ctgaaacata cggcgatctt
     5101 ggacacatct acggctatca atggcgtcac tgggaaacaa aagacggtgg ctttatcgat
     5161 caaatcaaac aagtgatcga agcaatcaaa aagactcctg attctagaag attgatcgtt
     5221 tctgcatgga atccggaaga tgttcctagc atggctttgc ctccttgtca tacgatgttt
     5281 caattttatg ttcaagaggg acggctaagc tgccaattgt atcaacgcag tggggatgtg
     5341 tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc tgactcacct aattgcacat
     5401 gaaacaggtt tagcggttgg cgaatttgtt cacacattag gagatgctca tctttatatc
     5461 aatcatatcg aacaaatgaa agaacaattg agccgagaaa tacgcgcatt tccgacttta
     5521 gttttgaatc aggaaaagca gtcagtattt gattttgaga tggaagatat ccaattagaa
     5581 ggatatgacc cacatccggc gatcaaagct cctatcgctg tttagaatca gaaaggatga
     5641 gaaaatgtta gctgcaattt gggcacagga tgaaaacgga ttgattggaa aagaagaccg
     5701 tcttccttgg cgtttgccaa atgatctgaa attcttcaaa caaatgacag aagcaaatac
     5761 actagtgatg ggacgaaaaa cattcgaagg gatgggtagc cgtccgttgc cgaatcgtca
     5821 gacgattgtt ttgacgagag attcttccta taaagcagaa ggcgtgcatg tcatgcatag
     5881 tgtggaagac gtgcttgctc tggaaaaaga aacagacggt atctttttca tcgctggagg
     5941 gtcagctgtt tatcaagaat ttctgccatt ttgtacgata ttataccgta cagtcatcca
     6001 tcatgtattc gatggagatg cttattttcc acctgttgac tggtctgatt ggtcactgat
     6061 caatactagt caaggagaaa tggatgaaaa aaatcgttat ccgcatcaat ttgaaacgta
     6121 tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa ttgacgatat aaaaaagcta
     6181 tggatagact agctgttatt tagagctgtc ccatccatag ctttattttt tttattaagt
     6241 aaagaataaa acagagaagt acataaaacc tgcacctaac ataacaaaca aatgccaaac
     6301 aacatgcata tagcggatag aattcaagct ataaaagaat gcgcctatcg tgtaagaaag
     6361 cccgcctgct accatcaaaa taacaccagt cattccaaga gagtgataaa gaggaacagc
     6421 agccacgaca cagagccacc ccataaaaat atagatgatc gtagatactt ttgatactct
     6481 ctcccgttta tgcaacgtca acgctttata tataatgcca agaatcgcaa acaaccagat
     6541 taatacaaat aaagtccaac ctagccatcc tcggatactc agcaaacaaa aaggagtata
     6601 acttcctgca atcaaaagaa aaatcgaact atgatcgaat acttggaaca ccttttttgc
     6661 ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa aataaaagaa tcatcatcga
     6721 tccatagata gcataagaga caatatggag ggaggaatgc aagtgggcac cttttactaa
     6781 aagaaccact agtcccgcga tactcaatcc agtcccaatt ccatgggtga ctgcattaaa
     6841 cacttcattt aatatttcat acgttcgatt aaatttcgtt ttttccatga aaacggctcc
     6901 tttactaggt gtaattaatg aaaaatcatt atttgtctgt tatactaaaa atagaaattg
     6961 tcttacatgt agtttaacat gaataaggca tcttgttaaa gaaagagaga gagaaaataa
     7021 tgacaaatgt aaaaatcgta acagattctt catgtacgat ggaaaaaaat gtacgcgatg
     7081 ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga cggaacagta tatcccgatg
     7141 atgaccaact agacggcgaa agatttatgg aaatgatggc gaaagcaaaa aatctgccca
     7201 aaacaagcca accaccaatt ggagaattca tctcgatgta tgatgagcta ggagaagacg
     7261 gcagtgaagt gatttcgatc catatgacaa aaggcttgag tggaacagta gaagcagcaa
     7321 gacaagccag taatctaaca aaaacgaaag taacggttat cgacagtgat accactgacc
     7381 aaggattatc ttttcaggta attcgagcag ctgaattagc aaaagaaggt aaaacagtag
     7441 agaaaatcct tgaagaagtc aaaaaagtac aagacaatac aaaattatac atcggaatat
     7501 ccacactaga taacttggta aaaggtggaa gaatcagccg aacaactggt ttgctgtcta
     7561 gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc tgaattaatc cctgtaacta
     7621 aggggcgagg aaagaaaacg ttcgataaat ggtttaataa tttgaaaaat gaactgaaag
     7681 aattgccaaa tgtgcgacaa atcggtattt cacatgcgga tgcaaaagaa ttggcgcaag
     7741 aattcaaaga gggtttgcaa gaattattcc cggacatgca tatccctgta ctgcatacga
     7801 atccggttat cgctacgcat actggtaaag acgcgtttgc aatcatgtat tatgtagatt
     7861 agtcgaaaag aggataagcc ctcagcctga aaggcttgca gcttattctc tttttcttta
     7921 taaaattagg aaaataattg ttttcttttt tcataaagac tacctttttg ataaaatttt
     7981 tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa tgaaattaac gaaacgacat
     8041 ttgatcctcg ttttgttccc attattaatt gcgatcatca tctatgcagg tttagctgct
     8101 tctataccga aagctggccc actaatgaaa ccagtaacta ctgtgagaga agatccaaat
     8161 aaaaaagaaa aacttcacta catagctatc ggagattcgc tgactgaagg agtaggagat
     8221 caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg atctaaaaga ccgttataat
     8281 ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg aacgaagtga ccagatctta
     8341 aagcgagtaa aaacaaaaga agaaattcga aacaatatca aaacagctga ttttatcaca
     8401 ctgactgttg gcggtaatga tttgatgaaa gttatccaaa ataatttatt cgggttaacg
     8461 gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa atgtcacaga actattggaa
     8521 gaatttcgat ccctcaataa aaccgcacct atttacattt taggcatcta caatccattt
     8581 tatttgaact ttcctgagat cactgacatg cagacaattg ttaacaattg gaacaaagga
     8641 acagaaacgg tgacaaaaaa aatgccgaac tgctacttta tcccaatcaa tgatttgctt
     8701 tatcaaggat tgaatcagga agtaggtgtc acagaagaag gaacagatca aacaactagc
     8761 acgacagaaa atctaaatgg catcaaaaat aatgtgctct atgaagaaga tcatttccat
     8821 ccaaataatt taggttatca attgatggca aatgcagtga gagatcagct gatcgagaca
     8881 caaaaccaat ggttagagaa ggaggatgat caataaacat gaaaacaaga tctgacgtat
     8941 cttcaaacaa aacaaagaaa acaaattact ggaaattcgc cttccttatc cttcttgggg
     9001 tagtacttgg ttcgaccgta tttttaggca gccgtatttt tgctaatcgg gaaccagaac
     9061 tcccagaaat accagctctt acggaaagac aaggagatcc tgtcttaacg atcaattcaa
     9121 ataaagaaaa agtcaatcaa atcatcagct ttttccttag tgagtatcaa aaagatagtg
     9181 atattgaata taaattttat ttggaaaatg aagcactact caatggtact ttcgaagtac
     9241 taggttttcc tatcgatttc tatttatatt ttgatccgta tgtgatggaa aacggaaatg
     9301 tccaactaaa agctagaagt ctttctatcg gaacattgag tttgccaatc aaagatgtga
     9361 tgaatatgat caagagaaac tataaattac cagattggat cgaagtcaat acagatgatc
     9421 tgactatcat gcttaggttg gatcaattcc gaatgcaaaa cgggatgtat attaaagcag
     9481 ataaaatcga cttagtaaat gatgacattc ggtttagtct atatttgcca aaagatacct
     9541 ctgatgcttc aaacaactca aataccaaca aaactagcac cagcagtact gaaagcaaga
     9601 caaaagaaaa taaaaaatca tcggatgagt agtattagag ggggaatcat aatggaacga
     9661 gcaatatttg caggaggctg cttctggtgt atgatccagc ctttcgacac gcttcctggt
     9721 atccatacag tgatgtcagg atatactgga gggcatgtac cagatccgac ttatgaacaa
     9781 gtaaaaagcc aaacgactgg acatacagaa gcagtggaga ttatctatga tccaacattg
     9841 atttcatatg aacaattagt agaactttac tggcagcaaa ctgatccaac tgacgctttc
     9901 ggacaattcg aggatcgcgg tgacaattat cgcccggtga tcttttattt gacagaagaa
     9961 cagcggatca ttgctgaaaa aagtaagaaa cagttagctg aaagtggacg tttcgaacag
    10021 ccaatcgtta caaaaatcga accagcccaa ccattttatc tagcagaaga agaccatcaa
    10081 gagtattata aaaagcatcc tgaaaatttt aaaagaaacc atgcacgccg ggctgccttt
    10141 atcgaagaaa attggaggaa cgaagggtga atcgaagctt ttttcattat ttaatgacac
    10201 tgcgaggcgg tggcacacat gatgctttat ctgttttcgc tacagaagcc ggtaaagaca
    10261 tccaatttcc taagcacagc tctagctacg aagaaatttc taattatctg gaactgaatg
    10321 tcgactattt gccgtccatg gatatctttg acgaagcatg ggaaaaatat ttagaaaata
    10381 atcatgacat ataagagata gttttgtact ttatgatcaa aaaagttttt tgtccaatag
    10441 cctaattgaa agaattattt ttatttcagc tatctagtcg catttttttg atatttttct
    10501 tataataaag aaagaatgaa aaaaagaggg atggattgat agagattgac aaaaaaacaa
    10561 cgctatatta atcgcttctg gcttgtattc aaatacttac ttgtttctgt attccttgcg
    10621 ttcatgttgc ggggattttt attcatcccg gtaccagtgg aaggcaattc aatggaaaac
    10681 gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt cggagatcag acgatttgat
    10741 attgttgttt ttcaattagc agatggcacg atttatatca aacgagtaat cggtcttcca
    10801 ggagaaaatg tcagctacca aaatgatcaa ttgaagatca atgggaaagt agttaaggaa
    10861 ccttatctaa ctaaaaacct gaaatcagat catgcaaatg catcctatac gacggacttt
    10921 acactacaag agctaacggg acaatccaag cttccggaag ataattattt tgtacttgga
    10981 gataatcgaa gagtttcaaa agatagtcgt tcgtttggca cgatcaataa aactgatatt
    11041 ctaggtaaag caagatttgt ctattatcca cttgatgaaa taaaatggat ccagtgagaa
    11101 acaataaagc gtgcagtctg ttgagactgc acgctttatt gtttaatcca aaatcagttt
    11161 actaccttag tacgtaaaat caaacagctc gtttaaacac ttttctagcc tctaaagttt
    11221 tgataaatga ataaacaacc attatttgaa tagggatttg gatcatattc ctgatgaatc
    11281 tacctagaaa aattactttg aaatctgcac caaacaaaac agacatcagc caagaattga
    11341 taaaaaggct gactgggatc ataatcaata attgggcagc gatgacggaa ggcaggttta
    11401 tctttttatg atataaaaat aagccattaa taaatccacc aaggaaagcc gtgataagaa
    11461 aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt taaagtgttc gacaacattt
    11521 caagcaaacc agcccagatc ggtccatata gataacccat aagcgccata cttaaaaatc
    11581 cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt gtataagatg acatcaaggg
    11641 ctaataaaag agcagaggtc gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt
    11701 cttttgcaat caaagtgttc aaaatattct ctcctttgtc agttaataat aaaaggatac
    11761 cataaaatca ccaatgtgtc atgacacatt ggtgatttac atacactggc atctaacaat
    11821 taaaaaaagg gtgagaagac tgcttttata aactgtaaaa aagagattgt acgaataaaa
    11881 aaaatagagg cggagaatac ttatcgtatc atccgccttt gtttttttgt tataaaaaac
    11941 gtcagattag ttatctgctt acatcttcat ccatacgggt tctaatgtat ttccgtctag
    12001 atcttggact tctaatgaaa acatttcttc ttcaggcatt cccatatcta cttcgtggaa
    12061 agtaccacca ttttcttttg caatttgagc aaattttttt acttcatccg cgttttcaac
    12121 actaaacgaa atcaaagcac cacttgtttt tttattatca gctatttctt tatcttttat
    12181 aaatttttta taaaaatcat gagagagaag cattatccaa aaagtgtcat cccataccat
    12241 tgaaccagct tcttcagtag aaaattcttc atttttttta aaacccagct tttcataaaa
    12301 agcaacggaa cgattgacat cagcaacagg aaaattaaca aaaaccatac tattcatttg
    12361 tttcgtctcc tttctgtttt gttcgtctat catctcttat ttaggaaacg ctgtcaaaaa
    12421 tttagattca tatatagaaa ccaaaataaa aatttgcatc atcaaaaaag tgaagatgtg
    12481 atagaatgag gactggttca ctttcactta actaatagat ggaatttctt tataataaaa
    12541 aagtgaattt atattgaaaa agaaaaaaac aagtaaagcc ggggaatatt ccggtatgaa
    12601 aggaatggag agaacaatga ccatccaatg gtttccggga catatggcaa aagcccgacg
    12661 agaagtttct gaaaaaatga aatatgtgga catcgtattt gaattggtag acgcacgatt
    12721 acctttatct tctcgtaatc caatgctaga tcagatcatt cagcaaaaac ctcgccttgt
    12781 tttattgaat aaagcggatc tggcagatcc acaacagaca aagctgtggc aagaatattt
    12841 tcgtaaacaa ggccatcttg cattagctat cacggcacaa gaaagtaaag gaattaaagg
    12901 tctagtacct gcggctaaag aagcattgaa agaaaaacgg gcaagagatg ctgctcgggg
    12961 gatcaagcct cgcgctattc gggcaatggt cttaggtatt ccaaatgttg ggaaatctac
    13021 gttgatgaat cgactagtag gcaagaaaat tgcacagaca ggaaataagc ctggcgtaac
    13081 aaaaggtcag cagtggcttc gtttaggtaa tgagttggaa ttgttagaca caccagggat
    13141 tttatggccc aaatttgaag atgaagaaat cggcaaaaaa ctagcgctga caggcgcgat
    13201 caaagaccag cttgtccatt tagatgattt agcgatttac ggattagact tttttgcccg
    13261 atattattca ggaagaatca gcgatcgtta tggtttatca atcgagcaag aagaaaaatt
    13321 ggcaccggaa ttactgatgg atattacagc aaaacgtggg tataaagaag actatgatcg
    13381 tggcagcgaa ttagtcgtgc atgatattcg tcaaggaaaa cttggacgat acacactgga
    13441 tcgttgcgaa gaaatggagc aaaaaagtga tgactgaatc catccaaagt atcaaagaac
    13501 gattgaaaag catacagaga atagatgatc ctacactaga atcattaaga aatgatccac
    13561 gcaaaggcgt gcagcaagca ctggcttctt ttgaacgacg attgaaaaag gaagcattaa
    13621 ttaaggaaaa atatgaagaa atgttttgct ttgaaaagca agggtacgca caaggtcacc
    13681 aatggattgc cggcattgat gaagtaggaa gaggaccatt agctggacct gtcgtggcag
    13741 ctgcagttat cttgccgaaa aatgagatca tattagggct gaatgactcc aaacaattat
    13801 cagagaaaaa gcgagaactt ctttatcaac aagttcaaga aaaagccctg gcaatcggga
    13861 tcgggatcgt tgatcagcag acaatcgatg atgtgaatat ttatgaagca agtaaattag
    13921 cgatgatcca agcagtagaa aaattgtcca ttgtaccaag ctatttgttg atcgatgcga
    13981 tgaccttgga tttgcctatt cagcaggaaa aaataatcaa aggggatgca cgatctgtgt
    14041 cgattgctgc tgcaagtatc attgctaaag tatatcgaga ccaattgatg aaagaatatg
    14101 acgtgcttta tcctggatat ggtttctcgc ataatgcagg ttatggaaca aaggaacact
    14161 tagaaggact tgaacgacaa ggaattacgc caatccatcg taaaagtttt gcacctgtaa
    14221 aaaaatattt acaataaaaa acagccgctt ttacgtatct tattagaaag agagacgaaa
    14281 ggcggtttta tgatgtatca aatagaagaa aatttattga aacttgctta tacaaaagga
    14341 atcagtagtc aaggaaaatg ggcacttgct aactggatga tgcagtatcc ttataaagaa
    14401 atcggatttg aagacatcat taagataggt aaaataacca aacatcggga gctgtttcgc
    14461 aaaagttggc aggaaattca cgctaactgg ggcaaaatcc aatcgaaaca gtcgtttatc
    14521 acttgctttg atcccaaata tccccctcag ctgcttcact tgacctatcc gccaattgta
    14581 ctgttttata atggtgatct atctttactt tcttgtaaca tgttaagcgt tgttggtgga
    14641 agacacactt ctgctttagc caaaaaaaca gtttattatt tactaaaacc agtgatagaa
    14701 gctggatatg tgatcgtcag tggctgcgcg aaagggatag atacatacgg tcaccaagca
    14761 gcaatctctc atggtggacg gacaatcgca gtcattggaa cagggataga gcaagtctat
    14821 cctgctgaaa atcgtgactt gcaagaggtg attgcaaatg atcatcttct tttgagtgaa
    14881 tatcctcctc aatccggccc gaaaaagcat tgttttccaa tgagaaatcg gattatcgca
    14941 gctttgtcta aaggaacatg tgtctttgaa gccaagaaaa aaagcggctc tctgataaca
    15001 gcacagcaag cattggatct tggaagaaca gttttttctg ttccaggaaa tattttaaca
    15061 gaacattcta ccggatgtca tcatttgatc caagacggtg cagtttgcac ggtttcgggt
    15121 gaagatatct tggcagaatt taaagaataa caccatactt ttcatttgct tttcttgaca
    15181 aacgttttct gaatggatat gattagctac gatttgtatg aatcctcttt taatagtagg
    15241 aacacgacaa tggaaaggag tccattcagt aatggcgtat aaatatttag tgattgtaga
    15301 atcacctgca aaagccaaga caattgagaa atatcttgga cgaaactata aagtaatggc
    15361 cagtgttggc catataagag atttaccgaa aagtaaaatg ggtatcgatt ttgaaaacaa
    15421 ttatgaaccc cattatattt ctatacgcgg aaaaggcgat gtcatcaaaa gcctgaaagc
    15481 cgcagcaaaa aaagctcaaa aagtttactt ggcaagtgac ccggatagag aaggagaagc
    15541 gattgcttgg catttagcgt acctacttgg gttggatctg aaagaaaaaa atcgggtggt
    15601 cttcaatgaa atcacaaaag acgcagtcaa agcagctttt aaggaaccaa gaacgatcga
    15661 tgtagattta gtagatgcac agcaagctcg tcgtacctta gacagaatcg ttggttattc
    15721 gatcagtcct attctctggc gtaaggtcaa gaaagggtta agtgcaggac gtgtccaatc
    15781 tgtcgcttta aaaattatta ttgaccgtga aaaagagatc cgagaatttg ttccagaaga
    15841 atattggagc atcgacggta attttaaaaa agctcgcaag aaattcaaag caaatttctg
    15901 gggaatcgac ggtaagaaaa agaaattacc agatgcacaa agtgtaaaag aagtcactgc
    15961 tagaattgat ggaacagact tcgaagttaa aaaagtagaa aagaaagagc gcaaaagaaa
    16021 ccctgcgaat cctttcacta caagtagcct gcaacaagaa gctgcccgta agttaaactt
    16081 tcgaacaaga aaaaccatga tggtagcaca gcaattatat gaaggaatca ctttgggtaa
    16141 acaaggaaca gtcggcttga ttacatacat gcgtactgat tctacccgga ttgccgatac
    16201 agcaaaagca gaggccgcag catttattga agatacgtat ggaaaagaat tttctagaca
    16261 tgatcaccga aagatcaaaa atgctcaagg cgcacaagat gcccatgaag ctgtgcgtcc
    16321 aacgagtgtg cttcgtacac cagatgaact caaacaatat ttagataaag atcaattgaa
    16381 actttataca ttgatctggt cacgttttgt ggctagtcag atgacaccag ctattttaga
    16441 tacaatgaaa gtgacattag aacaaaatgg tgtcatcttc attgcgaacg gttcaaaaat
    16501 caaattcaaa ggattcatgc aagtgtatgt ggaaggtaga gatgacggca aagaagagaa
    16561 agaaaatatt cttccagaac ttgaagaagg cgatgtagtc caatctgttg atattgaacc
    16621 aaaacaacat tttactcaac caccagcacg ttttagtgag gctacgttga ttcgtgcact
    16681 agaagaaaac ggggtagggc gaccttccac ttatgcacca acacttgata cgatccaaag
    16741 acgctactat gtaaaattga cacaaaaaag atttgagccg actgaactag gagaaattgt
    16801 caattcatta atctgtgaat tcttccctca aatcgtcgat atccatttca cagcagaaat
    16861 ggaaggagat ttggataaaa ttgaagaagg cacggaagcg tgggtcaaag tggttgatcg
    16921 tttctataaa ccgtttgaaa aagaactgac gaatgcggaa gaaaaaatcg aaaaaattca
    16981 aatcaaagat gaaccagcag gattcgattg tgatgtctgt ggacatccga tggtgataaa
    17041 attagggaaa tatgggaagt tttatgcttg cagtaatttt cctgattgtc ggaatacgaa
    17101 accaatcgta aaagaaatcg gtgtgacttg cccagtatgt catgaaggac aagtcattga
    17161 acgaaaatct aagaaaaaca gactattcta tggttgttcc cgtttcccag aatgtgaatt
    17221 cacttcttgg gataaaccag tgggcagaaa ttgtccaaaa tgtgacggat atctggtcga
    17281 gaagaaagtc aaaggcggta aacaagtagt ctgtgtaaat ggagattacg aagaagacat
    17341 ccaaaaataa tagaaaagag gttctttcat tgaaaatggt aaatgttgtt ggagcagggc
    17401 ttgccggaag tgaagctgct tggcagatcg cacaagcagg agtccctgtc cgtttatatg
    17461 aaatgcgtcc agtcaaatcg actgaggcgc atcacacaag taattttgct gaactagtat
    17521 gttccaattc attaagagga aacagtttgg ctaatgcagt agggttattg aaagaagaaa
    17581 tgcgccgatt gaattcggta gtaatcactt cggcagatga aacagcagtg ccagctggtg
    17641 gtgccttagc tgttgaccgc gattcttttt ctgaaacaat tacccgaaaa gtcaaagagc
    17701 atccattagt aacagtgata aacgaagaaa ttactgaaat accggaagga atcaccgtag
    17761 tagcaacagg acctttgact tctcatccat tagcagaaag catcaaagca ttcaatggtt
    17821 cagatggttt ttatttctat gatgcagctg caccaatcgt agacaaagct acgatcgata
    17881 tggataaagt atatttgaag tcccgctatg acaaaggaga agcagcttac ttgaactgtc
    17941 cgatgacaga agaggaattc aaagctttcc atgaggcact aatcaatgcc gaagtggcag
    18001 aacttaaatc ttttgagaaa gaaaaatatt ttgaaggatg tatgccaatt gaagtaatgg
    18061 cacagcgagg attcaaaacg atgcttttcg gacctatgaa accagttgga ttggaagatc
    18121 ctaaaactgg caaacgtcct tatgctgtta tccagctgcg tcaagacaat gctgcagctt
    18181 ctttatacaa catcgtgggc tttcagactc acttgaaatg gggcgagcaa aaacgtgttt
    18241 tccggatgat tcctgggtta gaaaatgctg aattcgttcg ctatggtgtg atgcaccgaa
    18301 acagctttat gaattcgcca gaattgctga aaccgactta ccaatcaacg aaacgcgaag
    18361 atctgttctt tgcaggtcag atgactggtg tagaaggcta tgttgaaagt gcagcgagcg
    18421 gactacttgc tggaatcaac gccgcacgct tagcaaaaga aatggaacca gtcgagtttc
    18481 cacaagaaac agctattgga agtatggctt actacattac tcatgcggaa ggaaaacatt
    18541 tccaaccgat gaatgccaac tatggtctgt ttcctgaact gccagagcgc attcgagaca
    18601 aaaaagcaag atatgaagca attgcacaac gagctttaaa agctaatgag gcaataaaaa
    18661 cagaattaga aaaaactgca atcccaaatg agtaaggaaa ctccatgaag agggtgagac
    18721 ggatagtctc gctcttttt
//
LOCUS       DACECS010000056        17943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.056, whole genome shotgun sequence.
ACCESSION   DACECS010000056 DACECS010000000
VERSION     DACECS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.056"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWO31_002014"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWO31_002014"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0302417.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWO31_002015"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWO31_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0302418.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWO31_002016"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWO31_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0302419.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWO31_002017"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWO31_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0302420.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWO31_002018"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWO31_002018"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0302421.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWO31_002019"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWO31_002019"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0302422.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IWO31_002020"
     CDS             complement(8052..8522)
                     /locus_tag="IWO31_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0302423.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IWO31_002021"
     CDS             complement(8568..8993)
                     /locus_tag="IWO31_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0302424.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IWO31_002022"
     CDS             complement(9060..9329)
                     /locus_tag="IWO31_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0302425.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IWO31_002023"
     CDS             complement(9340..10935)
                     /locus_tag="IWO31_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0302426.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWO31_002024"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWO31_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0302427.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IWO31_002025"
     CDS             complement(14488..15048)
                     /locus_tag="IWO31_002025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0302428.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IWO31_002026"
     CDS             15401..16375
                     /locus_tag="IWO31_002026"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0302429.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..>17943
                     /locus_tag="IWO31_002027"
     CDS             16545..>17943
                     /locus_tag="IWO31_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0302430.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4868 a         3973 c         3238 g         5864 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ctttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttcc cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatggaatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttacactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tct
//
LOCUS       DACECS010000057        17892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.057, whole genome shotgun sequence.
ACCESSION   DACECS010000057 DACECS010000000
VERSION     DACECS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.057"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(48..1514)
                     /locus_tag="IWO31_002028"
     CDS             complement(48..1514)
                     /locus_tag="IWO31_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0302431.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IWO31_002029"
     CDS             1901..2497
                     /locus_tag="IWO31_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0302432.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IWO31_002030"
     CDS             complement(2555..3037)
                     /locus_tag="IWO31_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302433.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IWO31_002031"
     CDS             complement(3143..3340)
                     /locus_tag="IWO31_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302434.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IWO31_002032"
     CDS             3635..4393
                     /locus_tag="IWO31_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0302435.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IWO31_002033"
     CDS             4453..4881
                     /locus_tag="IWO31_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302436.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IWO31_002034"
     tRNA            4927..4999
                     /locus_tag="IWO31_002034"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IWO31_002035"
     CDS             complement(5149..5520)
                     /locus_tag="IWO31_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0302437.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IWO31_002036"
     CDS             5735..5920
                     /locus_tag="IWO31_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302438.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IWO31_002037"
     CDS             complement(6003..6191)
                     /locus_tag="IWO31_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302439.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IWO31_002038"
     CDS             complement(6215..6478)
                     /locus_tag="IWO31_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302440.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IWO31_002039"
     CDS             6636..6893
                     /locus_tag="IWO31_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0302441.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IWO31_002040"
     CDS             complement(6964..7155)
                     /locus_tag="IWO31_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302442.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IWO31_002041"
     CDS             complement(7296..7616)
                     /locus_tag="IWO31_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302443.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IWO31_002042"
     CDS             complement(7639..8073)
                     /locus_tag="IWO31_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0302444.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..9046
                     /locus_tag="IWO31_002043"
     CDS             8408..9046
                     /locus_tag="IWO31_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0302445.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9370..9453)
                     /locus_tag="IWO31_002044"
     tRNA            complement(9370..9453)
                     /locus_tag="IWO31_002044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9417..9419),aa:Leu,seq:cag)
     gene            9981..10481
                     /locus_tag="IWO31_002045"
     CDS             9981..10481
                     /locus_tag="IWO31_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302446.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10671..11015)
                     /locus_tag="IWO31_002046"
     CDS             complement(10671..11015)
                     /locus_tag="IWO31_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0302447.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11169..12089)
                     /locus_tag="IWO31_002047"
     CDS             complement(11169..12089)
                     /locus_tag="IWO31_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0302448.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12276..12767
                     /locus_tag="IWO31_002048"
     CDS             12276..12767
                     /locus_tag="IWO31_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0302449.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12791..15166)
                     /locus_tag="IWO31_002049"
     CDS             complement(12791..15166)
                     /locus_tag="IWO31_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0302450.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15492..16118
                     /locus_tag="IWO31_002050"
     CDS             15492..16118
                     /locus_tag="IWO31_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0302451.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16141..16845
                     /locus_tag="IWO31_002051"
     CDS             16141..16845
                     /locus_tag="IWO31_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0302452.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17037..17870
                     /locus_tag="IWO31_002052"
     CDS             17037..17870
                     /locus_tag="IWO31_002052"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0302453.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5556 a         3489 c         3015 g         5832 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattca atttctgata ttaacctgat ctactcagga caacaattaa caatcccaac
     8641 agaaggctct gcacaagcag ctgctgaacc tcaagcagcc gttcaagaag caccagtcca
     8701 agctgaacct gttcaagctg aacaacctgt tgtacaagaa acggttcaaa cggaaacaca
     8761 agcagcgcct gttgctgaaa cacaacctgc tcctgctgta acagaaactg ctgctactcc
     8821 agcaagtaca agctctgcaa aagaatggat cgcacaaaaa gaatcaagcg gttcttacac
     8881 agcaacgaac ggacgttaca tcggccgtta ccaattagat tcttcttact taaatggtga
     8941 ctactctgct gcaaaccaag aaagagtagc agaacaatat gttacttcac gttatggttc
     9001 ttgggaagcg gcgaaagcat tctgggaagc aaacggttgg tactaagatt cattatttga
     9061 acgtttaaac acattcaaaa tatagtaaga ggtcatgcga tttattcttt cgcatgacct
     9121 ctttttgagt ttaagtttag tctttattcc ttaactatcg tttgccagta tttcttgtat
     9181 cagaatctat aaatctacta tttgaatcaa tctattaggt tttaaactgt cccaccttca
     9241 cacaaatttt ttttgattta tttgaatctg gttccgttgt ttaaaatttt tcctatattc
     9301 ttttagataa tatgttattt tctttctcat cgctccaggt gtaccttctc tttataaaaa
     9361 aacaaattat gcggctaaga agactcgaac ttccacgagg tcaccctcac tagcgcctga
     9421 agctagcgcg tctgccaatt ccgccatagc cgcatcacat cttttagttt aggattacat
     9481 gtgttttttg tcaagaacat atttttgttc tttttcattt ctgtacgccc tcccttatgc
     9541 catctacaca aataattcat ccgagcaact ccatcatcta ttgagggttg gtgaaaaaat
     9601 ctgttttatc catatgatcg atttcaatgt taaacgaacg actctgggat tagtaactat
     9661 tttatttttt tcttcatttc ttttttcctc tttttctcga ttcagctatc ttctttcatt
     9721 tggatattga ttatttcttc tgttgattac tatccttctg gaattctaga cgctgaagta
     9781 attcacgttt tctttcttct agagatcata agtaacgaat gaacgaactt caaataattt
     9841 ttttattgta attcatattt gtattgttcg atgattctta atacgatatg acacaataaa
     9901 taaaaatagg catggctatt tacaacaata gtgctatagt agaaaacaaa tgaataaggt
     9961 tacttaaaga ggtgaatacg atgaaactga tcaaactatg ggaagcagat ttaaagaaag
    10021 cttatcagct acaaactagc tttggaccaa atgagagtgg atttatcaac aatgcttatg
    10081 gctatacatt tgctgaattt ttagattatg taaaaaaatg cgaaaaaacg tctttaggaa
    10141 tcgatcttcc tgagagttac gttcccgcaa ccgtctttat tttagaaagt gatgaacatg
    10201 agtatgtagg aatttttaat ttgcgccata aactgaacga aacattaaga aacggcgctg
    10261 gccatattgg ttacggcatc tcccctaatt atcgtaaaaa aggatatgcc acacaaggat
    10321 tagcattagt actacaagag gcaaaaaaaa tcggcattga tgaggcactt ttgtctgtaa
    10381 ataaaaaaaa tcttggcagt cttaaagcac aacaaaaaaa tggggctgcg atttatcgtg
    10441 aagatgagaa agaatactat acgaaaatca aactgatgta aaaaagaagg attccaaaag
    10501 gaaagggatt tgaatgtcct ttatcaatag aacacattta tttaattaac ttataatata
    10561 gcagacaaaa ttttataaac aaagaaacct tttatctctt ttcttctaga aaggataaaa
    10621 ggcttttttg tttttcctat ttgcttctca tagctagtca gccaagattt ctatcgtatc
    10681 cccaacgcaa tccgtgcata acggctcatc ttatctgtcg tccatgctgg ataccagact
    10741 agtttgactt ctgctttcgt tacttctggc acttctttta atgcgtcatg gatagattct
    10801 gttaaaatat ctgctaatgg acagcccata gttgttagag tcatcttaat gactgtttct
    10861 ccattttcag gattgaattc tatatcataa atcaagccta aattgacgat gtcgattcct
    10921 aattcaggat caatcaccat ttccaaggca gcaagaatac gttctttgat tagttctatt
    10981 tcttgtccag accgttcttg gtttgcttcg ctcatcgtac gacactcctt aacacgattt
    11041 ttatttatca tactcttttt ttagctgctt gtaaatgaga atttagaagt aagattctcg
    11101 tttaaattta taaaaatgtt cttattagat gtaatagatt aaatttattc taaaataaac
    11161 ctgattcctc attttttttg tgaacgattt tttatttctg gcaatacgcc agttgttcga
    11221 tacatttttt gttcgttttc atttgcaaac cggccaaata ctgaatgagt tggtaaaata
    11281 ttgataaatg catcgtcgtc gatttcatga acgatctgat caagatcata catttcgtag
    11341 cgggtgatga ccatcatgat catgttccct tgtttttttg aatagccacc gacagacggc
    11401 agtaatgtca ttccacgtac catccgtttt gctagttctt ctgttacctc ttctggcttg
    11461 acggtaacga tcattgctgt tactttttga tgactcgtgt ggatggcatc aattacttga
    11521 gtcattgcat aaatggaaat aatcgtatac aaggcacttt cccagttaaa gaagaagcct
    11581 gcgatgacta caatgatacc atttagaatc agcatgtagt ttccgactgt ttttccagtt
    11641 gtttgtgaaa gtacaagaga gatgatatcc atcccaccag ttgtaaagcc catttttaaa
    11701 gataatccta cgccaagacc taataaaaca ccgcctacta gggcattcat caaaatatta
    11761 tcagtgatct gtccgactgg aagcagcatc gtcatcaccg atacacatac aacattcaca
    11821 aaactaagga tcgttgctcg ttttcctact ttgagaaacc ctaatacaaa cacagggata
    11881 ttcaaaagaa aaataaatgt tcctgtgtct atcttgatat gaaaccattc atataaaagc
    11941 gaggaaatga tttgagcgat cccattcatc cctgcagaaa agacattggc tggaatcaaa
    12001 aaataattta aagcgaccgc agcggtcaat ccggttatca aaataaccac tattttcttt
    12061 aatctttcat ttttatgata cgcagtcata attttactca caagcacctc tcccctactt
    12121 tggtattcca aatgtaagaa taacagttta tctattaaaa taaaatactt cgaactcaaa
    12181 tgagaagaat tttagcaaac aggtaagaat ctgccctaat cctatgataa gatagatgta
    12241 tggtaaatat ttatatgtat atcaggaggg ttcctatggc aaaagatgct agcttcgaca
    12301 ttgtatcaga agtcaataca gaagaagtaa aaaatgcgat tcagcaaaca acaaaagaat
    12361 tgtccaatcg ctttgacttc aaggattcta cagtagaaat caaattagag aacagcaatc
    12421 tagttgtatt gagcgatgat gatttcaaaa tcgaacagat caaagatgta ttatttggta
    12481 agctgatcaa aagaaatgtg ccaatcaaaa atatccattt ttccgaatca gaacacgctc
    12541 taggtggcaa agctcgacaa actgccgatt tggtcaacgg aatcgatcgt gaaaacgcga
    12601 agaaaatcac tacagctatc aaaaatgaaa aactgaaagt aaaagcacaa atccaagaag
    12661 atcagattag agtgacaggt aagaaccgtg atgatctaca agctgtgatt gctttattac
    12721 gtaaacttga cttaccaatc gagttgcaat tcactaacta tcgttaatca aaaaacgagg
    12781 ctgacgtctg tcattcctcg tttttttgat ttactgcttc ttttgataac caaaaacgct
    12841 gtattccttc ttccaattcc ccaacacttc gtacttctct tgctgtttgc caatgttgtg
    12901 ggaataaacg tcgataagaa aaactagaaa atcgttgatc tgccaataca acgattcctt
    12961 gatccgccat gccacgaatc actcgaccac cagcttgcaa gactttgttc attccaggaa
    13021 gctgataagc gtaagcaaat ccaaggtgtt ccttctcatc ataatatgat ttgatcagtt
    13081 cctgctcatg attcatctgc ggcaaaccta cacctacgat catactgccg atcaatcggt
    13141 cccctctcaa atcgattcct tctgaaaaaa tcccgccaag tacgcaaaat ccaatcaatg
    13201 tttcagcggg atttgtttga aattttgtta aaaatcgttc gcgttcttcc tcattcatgt
    13261 ccgtttcttg gattagcaca ttgctgtcgg gatattgctg tctgaatgct tccaccgcaa
    13321 gatctaaata ttgataagaa ggaaagaaaa cgaaataatt cccgaccttt ccatcagcaa
    13381 aagcatgaat cgtctctact aattttgcca gactgtgttc cctttttcga taagtcgtct
    13441 ctaggtaatt tgcgatgaaa acttgctggt ttccttctgg gaaaggactc ggcagtttgt
    13501 aagccagact tttttgtccc cccagtgttt cctgataata agagagtggt gaaaaactag
    13561 cagaaaaaag aatactgctt tttcctttat ccaacgactg ctcaagaaac aagcttggat
    13621 caatacaaaa ctcttttact atcagatcgc gatacgtttt ttcgactgtt gtttcgtagt
    13681 gatcatcata aaattcactg accttcaaaa aatgaagcaa atcaaaataa taaggaagca
    13741 attgttcttg tgatgggtgc tcaggaaatt ctgccagcca ttctttaatt ttttcgctta
    13801 attgataacc tgcctttact aaagattcag ccggtgcttt ttgatgatga taatcccaat
    13861 gatcttcttt tgctatttct cggatcgaat caaattcttt taatagtttg ttgaaacggc
    13921 gatgaagttt tttgaattcc tttggtatag cttccttact tcgtttcgtt ttttcataag
    13981 atagttctgc cgaatacatc tctcgggaac gattcaccaa attatgtgct tcgtcaatca
    14041 aaaacagata gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa acagttggat
    14101 caaataaata attgtaatcc ccaatgatta cgtcacacca gatactgaca tctaatgata
    14161 gttcgaaagg acaaagtgta tgctttcttg cataagtttc gatgacttca cgagtgatct
    14221 gattctcatg attcaaaaga tcccataaac cttcattaat ccgattgtaa taaccattgg
    14281 catatgggca ctgatctgga ttacaggtcg tctcatctaa aaagcagatc ttgtctttcg
    14341 ccgtcaaagt gacacttttt acttcagctc ctgtatcttt taatgctgtc atagcatctt
    14401 cagctacttg tctagttatt gttttagccg ttagataaaa gatcttttct ccctcttctt
    14461 cccctacagc tttcaaagcg gggaataacg tagagattgt ttttcctgtt ccggtaggtg
    14521 cttctacaaa caatttctgt ttcgtacgga tcgttttata agcagctacc gctagttcac
    14581 gctgaccttt cctatactca tcatggggga atttcaaagc catcaaagag gcattgcgta
    14641 cagtgcgcca gttttcttga aaaacaagcc attctttata atcttcagtc agctttttaa
    14701 aaaaagtttc taactgctct cttgtctgat gttcaacctt tctcgtgatc acttcttctg
    14761 ccgtctggaa gtaagtcaat tgcagattta tttcttgtaa atcatgttct tgactataga
    14821 tataggcata cacccttgct tgatgaaaga aaagttccac ttgttctggt tctaaatctt
    14881 caaatcgtgg ttcggaggtt ttgatttcat ctatataata aataccgtct cttttgaaaa
    14941 taccgtctgc acggccttca acgatcaggt aatattcatc taattgaact gttgttttta
    15001 aaaagacttc tttttcatag ttttctcccg catctttttg gagcttgcgg tggattctcg
    15061 ctccttctaa tgcagtatgg ttactttttt tacgattgtc gatacttcca cgtcgcaaaa
    15121 taaattctac taagtgccgc acagatattt ttgactgttt gatcatttcc accacctctt
    15181 tctctctaag attacctgaa aaaacctagt ttagcaaaaa gagctctctt tccaaacata
    15241 aaaaaacacc ctgctgatta ggcagggata taggatatag aatttggatt tttggatgaa
    15301 tctaatgtaa caaggacata ttaaaatgct atgaaaactt actttccttc tctttagaat
    15361 atccaaatac ttttcatata gtctgataat tcagataagt aaagagaaat aagtacctgt
    15421 ttttgtttcc tagcaatgaa tgcgttatac taatggatga aagaagtttt ggctatcaag
    15481 gaggaatgca catgcatatc gaaagattaa aaacaggaat gattgaagaa aactgttatt
    15541 tggtctacaa cgaagaagca cttttgatca ttgaccctgg agaagatgcg gaacgaatca
    15601 aacaagaaat caaaaaaaca aaccaacagc cggtagcgat tttgcttacc catactcact
    15661 acgatcatat cggtgcagtg gaacctttac gcgcgtatta tcaaatccca gtttatgtca
    15721 gcccactaga gcaaaaatgg ctaggtgatc cgattttgaa tctttctgga ctcggtagac
    15781 atgatgatat tgctgatgtt attgtctcgc ctgcagaata tgaatttgaa atgaaaactt
    15841 atactttagg aaacatgtct tttcgcgtcg tccctacccc agggcattct ataggaagcg
    15901 tcagctttat ttttgatgat tttgtcgttt ctggcgacgc cctgtttaga ggaagtatcg
    15961 gtagaaccga tctgtataca gggaatttag aacaattact tcatagtatt cggacccagc
    16021 tgtttgtatt acctgatgaa tttgcagtct atccaggaca tggcgatgca acgacgattg
    16081 aacaggaaaa aaggacaaat ccttttttca atcgttaaaa cgacttagga ggagaataaa
    16141 atgacagaaa ctacacttta tatcgtacga cacggaaaaa cgatgttcaa tacaattgaa
    16201 cgagtacaag gctggtgtga tacacccctc acaaagcaag gacaagaagg cattcattat
    16261 ttaggtcgcg ggttaaaaaa tgtcgacttt tcatttgctt attctagtga cagtggtcgt
    16321 gcaatcgaaa ctgcccgtat tattctagga gaacattcta agggaaaaga aattccttat
    16381 tttattgaaa aaaggattag agaatggtgt ttcgggtctc ttgaaggtgg atatgacatg
    16441 gaaatgtggg gtgtgatccc tcgtgtattg aattttaaaa catatgatga aatgtttacc
    16501 acagaagttt cttttgaaca aattgctaat gctgtataca acttagatac cgctgggtgg
    16561 gcggaaactt atgaaacatt gcgagaccgt gtatggacag gatttgaaga tatcgcccat
    16621 cactgcgaaa aaaatggtgg tggaaatgtt ttagtcgtat ctcatggttt gacgatcgcc
    16681 tttttgctta atctgatcga tcctaaacag cctgttcgtg ctgggctttt aaatggcagc
    16741 gtaacaaagg tcacttatgc aaacggtcga ttttctatcc acggcatcaa tgatacgtct
    16801 tatatcgata acgggaaaac acatagtcgc gactcgtttc tataatgggg atattttaat
    16861 ttttaccaga gaatcataaa aggcaaagtc tgaacgaggg ctttgtcttc ttttttctct
    16921 tcgtgtactt atctgcttta tttctcaagt ttttcttaat attaaaaaac agattgggtt
    16981 tctcaaaaaa taacggtata ctataagatg tatcttaatt gggaaggaag gaatttatgt
    17041 tttttgcaaa tattatcaaa gcagtgattc tcggtattat tgaaggaata acggaatggc
    17101 tgcccatcag tagtactggt catttgatcc tagcagatga attcatcaaa ctggatgcca
    17161 gttcacaatt tatgtcgatg ttcaatgtcg ttatccaatt aggggcgatc ttggctgtcg
    17221 tcgttcttta ttttcacaag ttaaacccat tttctccaag taaatcaacg gtagaaaaaa
    17281 aagatacgtg gacactttgg tttaaagtga tcgttgcttg cataccagcc atcatcattg
    17341 gtttgccttt ggatgattgg ttagaagcaa atttccacaa gttcttacca gttgccattg
    17401 ttttgatcgt ttacggtatt gcctttatta ttgtcgaaaa acgcaacaaa aacaaaacac
    17461 ctcgttggac ttctctgaat gactttacgt accaagcagc tcttcttgtt gggttattcc
    17521 aagtcttggc acttgttccc ggaacatccc gttctggcgc aacgatccta ggatctatct
    17581 tgattggtgc ttcgcgtttt gtcgctacag agttttcatt tttcttaggg attcctgtca
    17641 tgtttggcgc aagcggcttg aagatcgtca aatatttagc tgaagggaat tcattcaaaa
    17701 tggaagaaac tgttgttctt cttgtaggaa cattcgtatc ttttgtggtt tccgtcttag
    17761 ctatcaaatt cttactaagc tatttgaaga aaaacgactt taccgctttt ggctggtatc
    17821 gtattatcct tggtatcatt ttgattggtt attggtttat tgcaatgtag aaaaaaacag
    17881 aacacttctc tt
//
LOCUS       DACECS010000058        17457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.058, whole genome shotgun sequence.
ACCESSION   DACECS010000058 DACECS010000000
VERSION     DACECS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.058"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            76..1017
                     /locus_tag="IWO31_002053"
     CDS             76..1017
                     /locus_tag="IWO31_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0302454.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(1167..2063)
                     /locus_tag="IWO31_002054"
     CDS             complement(1167..2063)
                     /locus_tag="IWO31_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0302455.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            2332..3672
                     /locus_tag="IWO31_002055"
     CDS             2332..3672
                     /locus_tag="IWO31_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0302456.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            3890..4333
                     /locus_tag="IWO31_002056"
     CDS             3890..4333
                     /locus_tag="IWO31_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0302457.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="IWO31_002057"
     CDS             complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="IWO31_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0302458.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(6658..7881)
                     /locus_tag="IWO31_002058"
     CDS             complement(6658..7881)
                     /locus_tag="IWO31_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0302459.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(7912..8751)
                     /locus_tag="IWO31_002059"
     CDS             complement(7912..8751)
                     /locus_tag="IWO31_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0302460.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(8768..9448)
                     /locus_tag="IWO31_002060"
     CDS             complement(8768..9448)
                     /locus_tag="IWO31_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302461.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(9450..10523)
                     /locus_tag="IWO31_002061"
     CDS             complement(9450..10523)
                     /locus_tag="IWO31_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0302462.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(11170..11841)
                     /locus_tag="IWO31_002062"
     CDS             complement(11170..11841)
                     /locus_tag="IWO31_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0302463.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="IWO31_002063"
     CDS             complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="IWO31_002063"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0302464.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            15265..15702
                     /gene="ndk"
                     /locus_tag="IWO31_002064"
     CDS             15265..15702
                     /gene="ndk"
                     /locus_tag="IWO31_002064"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0302465.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(15724..16590)
                     /locus_tag="IWO31_002065"
     CDS             complement(15724..16590)
                     /locus_tag="IWO31_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0302466.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(16750..>17457)
                     /locus_tag="IWO31_002066"
     CDS             complement(16750..>17457)
                     /locus_tag="IWO31_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0302467.1"
                     /translation="YLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEF
                     DNAQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQ
                     HDQVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLS
                     DKLNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTT
                     NLYPVTRTDVEQQKNNQ"
BASE COUNT         5077 a         3577 c         3090 g         5713 t
ORIGIN      
        1 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
       61 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
      121 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
      181 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
      241 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
      301 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
      361 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
      421 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
      481 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
      541 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
      601 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
      661 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
      721 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
      781 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
      841 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
      901 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
      961 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
     1021 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
     1081 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
     1141 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
     1201 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
     1261 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
     1321 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
     1381 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
     1441 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
     1501 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
     1561 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
     1621 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
     1681 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
     1741 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
     1801 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
     1861 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
     1921 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
     1981 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
     2041 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
     2101 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
     2161 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
     2221 ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta aatattgcta
     2281 tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat
     2341 aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca
     2401 atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta
     2461 ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa
     2521 atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta
     2581 ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc
     2641 atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc
     2701 tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg
     2761 ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg
     2821 atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc
     2881 cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt
     2941 tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc
     3001 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc
     3061 atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta
     3121 gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca
     3181 gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg
     3241 tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa
     3301 ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt
     3361 ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
     3421 ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta
     3481 ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc
     3541 ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact
     3601 ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg
     3661 acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt
     3721 gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata aacagtctca
     3781 tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat tgttctccat
     3841 gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta
     3901 ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta tgatgtactt
     3961 gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca tggcgttgat
     4021 gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa
     4081 aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt ccttgtttat
     4141 ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga ttcctcatca
     4201 ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt
     4261 agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa
     4321 agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag aagttatcag
     4381 cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc
     4441 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat ttttcgtttg
     4501 gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat
     4561 cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca tattttgcca
     4621 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa
     4681 aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca catttttgta
     4741 taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt aaaagggatt
     4801 gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt
     4861 gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact
     4921 gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt
     4981 aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt
     5041 gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt
     5101 ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat
     5161 aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt
     5221 catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc
     5281 acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc
     5341 gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat
     5401 gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc
     5461 tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg cacacagtaa
     5521 cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc
     5581 agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa
     5641 taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct tgatgtgttt
     5701 gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat
     5761 cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaagcc
     5821 tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact
     5881 ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac
     5941 actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac
     6001 agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg gtttgatgcc
     6061 attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat
     6121 gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc
     6181 aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt
     6241 tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta
     6301 agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa aataaaaaga
     6361 cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt
     6421 ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc
     6481 ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga
     6541 aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata
     6601 ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta ttttttacta
     6661 gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct
     6721 ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc
     6781 aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg
     6841 tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct
     6901 gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc
     6961 tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat
     7021 gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc
     7081 agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc
     7141 tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc
     7201 gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat
     7261 tccattaaac acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc
     7321 tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt
     7381 cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa
     7441 accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt
     7501 gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta tgctggcggc
     7561 taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc
     7621 atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat aacgtgcatc
     7681 aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt tttctaattg
     7741 tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat
     7801 atctagagca agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt
     7861 tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct
     7921 cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac
     7981 tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt tttcatcttc
     8041 ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa
     8101 gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga
     8161 aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag
     8221 attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac
     8281 tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt
     8341 agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata
     8401 aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt
     8461 attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc
     8521 taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc
     8581 ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa taatccctag
     8641 ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa
     8701 ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc
     8761 tccactttca atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa
     8821 ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc
     8881 ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca
     8941 gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta
     9001 acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct gccgcttcga
     9061 tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa
     9121 ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac
     9181 gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga
     9241 tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca
     9301 ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct gtccaaaaag
     9361 acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg
     9421 gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga
     9481 ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc
     9541 aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc
     9601 gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg
     9661 tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc
     9721 catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg
     9781 tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag
     9841 atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc taccttgttg
     9901 cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg
     9961 cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg
    10021 gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact
    10081 cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg
    10141 acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac
    10201 tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga
    10261 aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaga
    10321 ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga
    10381 actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca
    10441 atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca
    10501 gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga
    10561 aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc
    10621 tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag
    10681 ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc
    10741 gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac
    10801 catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt
    10861 aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct caagcccaac
    10921 atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa tttgcctact
    10981 tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc ttctcaccag
    11041 ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat cgatattttg
    11101 ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt caaattattt
    11161 tttctatatt tatttttctg tttttttatt taacatttca ataacagttc gaagatcttt
    11221 tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc ggacaaaact
    11281 ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa
    11341 agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca aagctgtcag
    11401 ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa
    11461 gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa cagtggtcgt
    11521 agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt ttgcatatgg
    11581 ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg
    11641 gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa aactttgttt
    11701 ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat caaattgctg
    11761 aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca aaccatctaa
    11821 atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac acatagttta
    11881 gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta taagagttct
    11941 tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgatgact
    12001 gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa ttcagcacca
    12061 aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat aacaaacatg
    12121 gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc atggagcact
    12181 aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc gatgaaaatc
    12241 actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg acaaataaca
    12301 aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc aaagtaaacc
    12361 atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata ttctttatcc
    12421 atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa aaacaaaagt
    12481 tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac ggaaaacata
    12541 ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc aaaagtttct
    12601 cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct
    12661 gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc agccactttc
    12721 attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc atttttacgt
    12781 aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac ttcatggtct
    12841 tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc ggtaaccaat
    12901 tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc gttgtcccca
    12961 gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg tttcgttgtt
    13021 tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga ctcatctttg
    13081 actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat cactcgtaag
    13141 ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc ttttgttaat
    13201 ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg
    13261 ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact catacgacga
    13321 cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc tgtatctaaa
    13381 gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag
    13441 tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc acaatccata
    13501 tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt acataacaca
    13561 tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc ttttttggcc
    13621 atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt
    13681 agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc
    13741 ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag tagcatggat
    13801 gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag cgtttctgct
    13861 acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc acttccactg
    13921 atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg ctgatcgtct
    13981 tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgggaa
    14041 atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc ccctgctgaa
    14101 agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta ctttccatta
    14161 cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc ttgattggaa
    14221 cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa gataatggct
    14281 acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat gaatgaaata
    14341 attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa tatttctaca
    14401 atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc tcttgaacgt
    14461 tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa taattcttgt
    14521 ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctca
    14581 atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt agctattcat
    14641 cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag ccctggcctt
    14701 tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa
    14761 gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca attagaccac
    14821 ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct cgccaaatac
    14881 tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat cgttgagttt
    14941 tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga aaagccttat
    15001 ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag tagcctatgt
    15061 ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat ataacaacta
    15121 aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa atacacacgt
    15181 tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct ataatagaaa
    15241 tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa gccagacggc
    15301 gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg actggccatt
    15361 gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta tcagcacttg
    15421 actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc tgtcgtttat
    15481 cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg agcaacaaaa
    15541 gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg gactgaaaac
    15601 atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg
    15661 ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct
    15721 ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc tccgttgatt
    15781 ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg tcttttcttc
    15841 ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt catagatcac
    15901 tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac tacgtgcctt
    15961 tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag taaagagata
    16021 ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt tcaaataatt
    16081 gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag atagttcaaa
    16141 ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt cataacgtaa
    16201 aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat tcaaaaccaa
    16261 ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct ctttataagg
    16321 atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta actttattgg
    16381 ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca ttcgctctaa
    16441 aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa tcccgactag
    16501 agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct tgttgactcg
    16561 aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt taaatttttc
    16621 cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt
    16681 tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac tgaaggttgg
    16741 tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta ctggatataa
    16801 gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt cgcctttttg
    16861 cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt tcggatcaat
    16921 cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga
    16981 aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat cgattcctgc
    17041 tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt
    17101 gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat gctgagaaga
    17161 taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa
    17221 tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt tagtataacc
    17281 aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt
    17341 atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg agatcccaaa
    17401 atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaata
//
LOCUS       DACECS010000059        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.059, whole genome shotgun sequence.
ACCESSION   DACECS010000059 DACECS010000000
VERSION     DACECS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.059"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IWO31_002067"
                     /pseudo
     CDS             1..368
                     /locus_tag="IWO31_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IWO31_002068"
     CDS             548..1489
                     /locus_tag="IWO31_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0302468.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IWO31_002069"
     CDS             1558..2289
                     /locus_tag="IWO31_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0302469.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IWO31_002070"
     CDS             2433..4613
                     /locus_tag="IWO31_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0302470.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IWO31_002071"
     CDS             4633..5115
                     /locus_tag="IWO31_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0302471.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IWO31_002072"
     CDS             5131..5892
                     /locus_tag="IWO31_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0302472.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IWO31_002073"
     CDS             6023..7357
                     /locus_tag="IWO31_002073"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0302473.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IWO31_002074"
     CDS             7785..8270
                     /locus_tag="IWO31_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0302474.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IWO31_002075"
     CDS             8263..8946
                     /locus_tag="IWO31_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0302475.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IWO31_002076"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IWO31_002076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0302476.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IWO31_002077"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IWO31_002077"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0302477.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IWO31_002078"
     CDS             12562..13191
                     /locus_tag="IWO31_002078"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0302478.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IWO31_002079"
     CDS             13243..13575
                     /locus_tag="IWO31_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0302479.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IWO31_002080"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IWO31_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0302480.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IWO31_002081"
     CDS             14401..15774
                     /locus_tag="IWO31_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0302481.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IWO31_002082"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IWO31_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0302482.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IWO31_002083"
     CDS             16829..17269
                     /locus_tag="IWO31_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0302483.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACECS010000060        17232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.060, whole genome shotgun sequence.
ACCESSION   DACECS010000060 DACECS010000000
VERSION     DACECS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.060"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(97..1116)
                     /locus_tag="IWO31_002084"
     CDS             complement(97..1116)
                     /locus_tag="IWO31_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0302484.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="IWO31_002085"
     CDS             complement(1138..2145)
                     /locus_tag="IWO31_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0302485.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IWO31_002086"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IWO31_002086"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0302486.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="IWO31_002087"
     CDS             complement(2796..5090)
                     /locus_tag="IWO31_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0302487.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="IWO31_002088"
     CDS             5350..7515
                     /locus_tag="IWO31_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0302488.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="IWO31_002089"
     CDS             7819..8652
                     /locus_tag="IWO31_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0302489.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="IWO31_002090"
     CDS             complement(8754..10454)
                     /locus_tag="IWO31_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302490.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="IWO31_002091"
     CDS             10961..12121
                     /locus_tag="IWO31_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0302491.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="IWO31_002092"
     CDS             12137..12580
                     /locus_tag="IWO31_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302492.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="IWO31_002093"
     CDS             complement(13063..13593)
                     /locus_tag="IWO31_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0302493.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="IWO31_002094"
     CDS             13698..14249
                     /locus_tag="IWO31_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0302494.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="IWO31_002095"
     CDS             complement(14402..14875)
                     /locus_tag="IWO31_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0302495.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="IWO31_002096"
     CDS             15013..16485
                     /locus_tag="IWO31_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0302496.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="IWO31_002097"
     CDS             16482..17192
                     /locus_tag="IWO31_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0302497.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5359 a         3096 c         3544 g         5233 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc gtacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag gatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgacactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggaaaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataagt actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtcaggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atctcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt tctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctgttttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaacaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacaccaacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg cttgatccaa
    15361 gcttgcggcg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tc
//
LOCUS       DACECS010000061        17150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.061, whole genome shotgun sequence.
ACCESSION   DACECS010000061 DACECS010000000
VERSION     DACECS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.061"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWO31_002098"
     CDS             138..1475
                     /locus_tag="IWO31_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0302498.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWO31_002099"
     CDS             1523..3652
                     /locus_tag="IWO31_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0302499.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWO31_002100"
     CDS             3627..4316
                     /locus_tag="IWO31_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302500.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWO31_002101"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWO31_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWO31_002102"
     CDS             complement(5546..5740)
                     /locus_tag="IWO31_002102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302501.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWO31_002103"
     CDS             complement(5790..6230)
                     /locus_tag="IWO31_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0302502.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWO31_002104"
     CDS             complement(6234..7079)
                     /locus_tag="IWO31_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0302503.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWO31_002105"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWO31_002105"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0302504.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWO31_002106"
     CDS             complement(8703..9650)
                     /locus_tag="IWO31_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0302505.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWO31_002107"
     CDS             complement(9873..10223)
                     /locus_tag="IWO31_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0302506.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWO31_002108"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWO31_002108"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0302507.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWO31_002109"
     CDS             11646..11966
                     /locus_tag="IWO31_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0302508.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWO31_002110"
     CDS             11988..12452
                     /locus_tag="IWO31_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0302509.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWO31_002111"
     CDS             12657..13262
                     /locus_tag="IWO31_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0302510.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWO31_002112"
     CDS             complement(13301..14590)
                     /locus_tag="IWO31_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0302511.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWO31_002113"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWO31_002113"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0302512.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="IWO31_002114"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="IWO31_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17150
                     /locus_tag="IWO31_002115"
     CDS             16321..>17150
                     /locus_tag="IWO31_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAQ0302513.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5459 a         3105 c         3467 g         5119 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DACECS010000062        17022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.062, whole genome shotgun sequence.
ACCESSION   DACECS010000062 DACECS010000000
VERSION     DACECS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.062"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWO31_002116"
     CDS             226..894
                     /locus_tag="IWO31_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0302514.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWO31_002117"
     CDS             complement(1094..1480)
                     /locus_tag="IWO31_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302515.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWO31_002118"
     CDS             complement(1452..2834)
                     /locus_tag="IWO31_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0302516.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWO31_002119"
     CDS             complement(2990..4177)
                     /locus_tag="IWO31_002119"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0302517.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWO31_002120"
     CDS             complement(4184..5215)
                     /locus_tag="IWO31_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0302518.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWO31_002121"
     CDS             complement(5231..6685)
                     /locus_tag="IWO31_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0302519.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWO31_002122"
     CDS             6860..7765
                     /locus_tag="IWO31_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0302520.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWO31_002123"
     CDS             7856..8533
                     /locus_tag="IWO31_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0302521.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWO31_002124"
     CDS             8814..9710
                     /locus_tag="IWO31_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0302522.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWO31_002125"
     CDS             complement(9765..11135)
                     /locus_tag="IWO31_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0302523.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWO31_002126"
     CDS             11373..12119
                     /locus_tag="IWO31_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302524.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWO31_002127"
     CDS             12116..12955
                     /locus_tag="IWO31_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0302525.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWO31_002128"
     CDS             12956..13906
                     /locus_tag="IWO31_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0302526.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWO31_002129"
     CDS             14047..14799
                     /locus_tag="IWO31_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302527.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWO31_002130"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWO31_002130"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0302528.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16353..16961
                     /locus_tag="IWO31_002131"
     CDS             16353..16961
                     /locus_tag="IWO31_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0302529.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5560 a         3172 c         3392 g         4898 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tatcaaaaaa atatttgttt aaatgttaca aatattaaat aattcgttat tttgtcatta
    16261 aatgtcatgt ttcttttacc tatgaaatat ttctaaatga taaagttaat agcgtcgaaa
    16321 gaataaaaaa cggagaaaaa aaggagttta atatgaattc atttaagaaa attgttttag
    16381 gtacaacttt tgctgctggt gctactgcaa tgtttgtagg aactacaaat gctcacgctg
    16441 atgaagttta tacagtaaaa tcaggtgatt cattatctaa gatctcacaa aaatttgcag
    16501 gagacaactc aatgatcgat gcaattgctg aaaaaaattc gatcgcaaac atcaaccgca
    16561 tttatgttgg tgaacaatta acaatcccaa cttcaaatga ttcttcagca acaacagaaa
    16621 acaaaacaac agaaaataca gcatctacaa ctgaaacagc aacgcaagaa catacatacg
    16681 ttgcacctgt tgaaacagtt gaagtagctc ctgctgcccc agctgctgca acagcgccaa
    16741 cttcaagctc tgcaaaagaa tggatcgcac aaaaagaatc aggcggttca tacacagcta
    16801 ctaacggacg ttacatcggc cgttatcaat tagatgcttc atacttaaac ggcgactatt
    16861 cagctgccaa tcaagaacgt gtagcagaac aatatgtaac atctcgttat ggttcatggg
    16921 atgctgctaa aacattctgg ttagcaaacg gctggtacta agccgataaa aggaaaatga
    16981 tgaaaccgag aaacttgttt attcaagttc tcggtttttt tg
//
LOCUS       DACECS010000063        16819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.063, whole genome shotgun sequence.
ACCESSION   DACECS010000063 DACECS010000000
VERSION     DACECS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.063"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..455)
                     /locus_tag="IWO31_002132"
     CDS             complement(<1..455)
                     /locus_tag="IWO31_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0302530.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="IWO31_002133"
     CDS             complement(560..1423)
                     /locus_tag="IWO31_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0302531.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="IWO31_002134"
     CDS             complement(1619..3718)
                     /locus_tag="IWO31_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0302532.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="IWO31_002135"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="IWO31_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0302533.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="IWO31_002136"
     CDS             complement(5071..6912)
                     /locus_tag="IWO31_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0302534.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9487)
                     /locus_tag="IWO31_002137"
     CDS             complement(7094..9487)
                     /locus_tag="IWO31_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0302535.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="IWO31_002138"
     CDS             complement(9594..10454)
                     /locus_tag="IWO31_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0302536.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="IWO31_002139"
     CDS             complement(10513..11034)
                     /locus_tag="IWO31_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0302537.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11354..12073
                     /locus_tag="IWO31_002140"
     CDS             11354..12073
                     /locus_tag="IWO31_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0302538.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="IWO31_002141"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="IWO31_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0302539.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="IWO31_002142"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="IWO31_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0302540.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="IWO31_002143"
     CDS             16433..16789
                     /locus_tag="IWO31_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0302541.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5766 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaa
//
LOCUS       DACECS010000064        16590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.064, whole genome shotgun sequence.
ACCESSION   DACECS010000064 DACECS010000000
VERSION     DACECS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.064"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..786)
                     /locus_tag="IWO31_002144"
     CDS             complement(79..786)
                     /locus_tag="IWO31_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0302542.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="IWO31_002145"
     CDS             complement(865..1203)
                     /locus_tag="IWO31_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0302543.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="IWO31_002146"
     CDS             complement(1314..2276)
                     /locus_tag="IWO31_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0302544.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="IWO31_002147"
     CDS             complement(2291..3622)
                     /locus_tag="IWO31_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0302545.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="IWO31_002148"
     CDS             complement(3630..4310)
                     /locus_tag="IWO31_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0302546.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="IWO31_002149"
     CDS             complement(4529..5008)
                     /locus_tag="IWO31_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0302547.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="IWO31_002150"
     CDS             complement(5135..6814)
                     /locus_tag="IWO31_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0302548.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="IWO31_002151"
     CDS             7362..8864
                     /locus_tag="IWO31_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0302549.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="IWO31_002152"
     CDS             8877..9539
                     /locus_tag="IWO31_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302550.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="IWO31_002153"
     CDS             complement(9640..9843)
                     /locus_tag="IWO31_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0302551.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO31_002154"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO31_002154"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0302552.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO31_002155"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO31_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0302553.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="IWO31_002156"
     CDS             complement(13807..14421)
                     /locus_tag="IWO31_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0302554.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="IWO31_002157"
     CDS             complement(15027..15404)
                     /locus_tag="IWO31_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302555.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO31_002158"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO31_002158"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0302556.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4807 a         3574 c         2833 g         5376 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DACECS010000065        16428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.065, whole genome shotgun sequence.
ACCESSION   DACECS010000065 DACECS010000000
VERSION     DACECS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.065"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(28..591)
                     /locus_tag="IWO31_002159"
     CDS             complement(28..591)
                     /locus_tag="IWO31_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0302557.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(606..1649)
                     /locus_tag="IWO31_002160"
     CDS             complement(606..1649)
                     /locus_tag="IWO31_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302558.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1782..2186)
                     /locus_tag="IWO31_002161"
     CDS             complement(1782..2186)
                     /locus_tag="IWO31_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0302559.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2419..3054)
                     /locus_tag="IWO31_002162"
     CDS             complement(2419..3054)
                     /locus_tag="IWO31_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0302560.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3320..4372)
                     /locus_tag="IWO31_002163"
     CDS             complement(3320..4372)
                     /locus_tag="IWO31_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0302561.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4385..5323)
                     /locus_tag="IWO31_002164"
     CDS             complement(4385..5323)
                     /locus_tag="IWO31_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0302562.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5774..6424)
                     /locus_tag="IWO31_002165"
     CDS             complement(5774..6424)
                     /locus_tag="IWO31_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302563.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6524..7033)
                     /locus_tag="IWO31_002166"
     CDS             complement(6524..7033)
                     /locus_tag="IWO31_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0302564.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(7234..8103)
                     /locus_tag="IWO31_002167"
     CDS             complement(7234..8103)
                     /locus_tag="IWO31_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0302565.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(8123..9244)
                     /locus_tag="IWO31_002168"
     CDS             complement(8123..9244)
                     /locus_tag="IWO31_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0302566.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(9246..9641)
                     /locus_tag="IWO31_002169"
     CDS             complement(9246..9641)
                     /locus_tag="IWO31_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0302567.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(9660..10457)
                     /locus_tag="IWO31_002170"
     CDS             complement(9660..10457)
                     /locus_tag="IWO31_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0302568.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(10459..11247)
                     /locus_tag="IWO31_002171"
     CDS             complement(10459..11247)
                     /locus_tag="IWO31_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0302569.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(11261..11737)
                     /locus_tag="IWO31_002172"
     CDS             complement(11261..11737)
                     /locus_tag="IWO31_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0302570.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(11809..14466)
                     /locus_tag="IWO31_002173"
     CDS             complement(11809..14466)
                     /locus_tag="IWO31_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0302571.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(14485..14889)
                     /locus_tag="IWO31_002174"
     CDS             complement(14485..14889)
                     /locus_tag="IWO31_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAQ0302572.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(15121..15378)
                     /locus_tag="IWO31_002175"
     CDS             complement(15121..15378)
                     /locus_tag="IWO31_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAQ0302573.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(15503..15835)
                     /locus_tag="IWO31_002176"
     CDS             complement(15503..15835)
                     /locus_tag="IWO31_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302574.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         5129 a         3376 c         2414 g         5509 t
ORIGIN      
        1 ataaaatgga tgctaggtcc gttatattta ctcaaaattg attctacgaa tagatcttct
       61 tatcatttct attttagtag tcatgcgttg ttgttcttct aaaggtggta atggtataag
      121 tagtttagat aaatttgtac catttacatt tggttgccca gttccgtaag aaattttttc
      181 taataacttc caatataatg gcgagtctaa gaagcagtct acgtattctg ataataattt
      241 ctcaactaac ctaattcgaa tcaagtaaga agcaaaaaca gattcctcac ttatttcctt
      301 aaccaagaaa ctttttccca tagttcctcc tgtccttgct atcaaaatgt cattttcttc
      361 caatcttaaa tcaactagtt tagaattact aatatcacag taaggcacac tggaccaatt
      421 tacacgtccc tcttgtatat ctgtgattct caaaaactta acattgcctt gttttttcgc
      481 cgaatctgta tagccatatt gaatcttagt agaaatactt cctaaataaa cccatagtcg
      541 aacatcgcta gatgattttt cttgtcgttc tttatgaaca acagtgatca tcttgagttg
      601 tccgactatt ttttagttgc agcaatcttt ttgggacgct tatttgcatg aagcaacgtt
      661 gctaagtcta gtaataaaac attggctaac acaggattgg ctttctcgcg atacgctgcg
      721 gcgccacttt caaacaatac tgctagacga tcttctacaa caacgatttg ttccaaatac
      781 ggtggaaatt tgattcgttt taggatccgg ctaggagagt cccgctcgga cactaagagt
      841 tccgaggtat aaggaccata agaacgactg gctaagagat aatggccata aaaagccaca
      901 ccttgaatct tatcagatgt ttttctgctc attgtcacgg ttggttggat tttgtctttg
      961 cggcgaattg ctgttttcat ttcagctatc gtcggcaata gatagaaagt caactgtcct
     1021 tcacctttta gtgtgaagtt gccagatacc agttgatcat tgttttgagt caaatacgat
     1081 gcttgtggaa tctctgagag atcagttgtc agccaataag cgattggctg tcccgtctct
     1141 tcagatgtat ctgctaagat atcgatcaaa cgtaaagcgg ctactcgtcc agtagcagat
     1201 cctgtgattg tcaaccataa aagttcccgt tttgtatcat atactaagcc ccctacatgt
     1261 ggccgtttag gtaaaatcag cagtttgatc ggctgtcctg tttctctatc cagtacatga
     1321 ataatagaat gatgctgatg gtcatagcag taggcagaaa taaacacaaa atcttgagaa
     1381 aaagccaacc cttgcggcgt catccattga caaacctcat tttcgccggt ttccggcacc
     1441 accattcttg tttctgttaa tccaggaatg ttatattgtt ttgcttcagc cttttcccaa
     1501 gctgcttgaa caataggata ccgttttaac tttttttcaa tatcctgatg tatccgttgc
     1561 tgcttccaat gcttaaactt taggacatag taaacaagaa ggccaatgac cagcaacact
     1621 aaaatcatta ttgctacccg ctgtgtcatt tttttgcctc cttgttctat tcgatcaatc
     1681 gtccatatac cgctattaat taccgtaact acatgtttct tttactagcg tttctttata
     1741 ggatctttca taatctcatt tatagtaaca ttaagaatcc cttattctac tatagtcact
     1801 tccacatcaa tattgcctct tgttgcttta gagtatgggc aaaatgcgtg ggcttcttct
     1861 gcgattttct ctgcttggtc cttctcaagt cctggcaaag aaacttctaa aacgaccccg
     1921 atttggtagc ccccatcttc taattgaaat aaagataccc gtgctttgac ttcagaatca
     1981 gactcaagat tattttcttc cagcatgtgt tgaaccgcgc cgttgaagca agatgcgtat
     2041 ccagccgcaa aaagttgttc cggatttgtc ccttcactat ggattcctgg gcggtcgatc
     2101 ttcatgtgca tacttccgtt tggtgcctca acttcgccat cacggcctcc acgattgatc
     2161 attgctgttt cgtacatttt tttcattgag atcatccttt ctttcgtcgt tctatattaa
     2221 gtgtaacgag tattttttaa tctgacaaag gtaatgcttg cattcggcga catcagttag
     2281 acaacatcga tgatcagtga tagaagcagt ttatctatac aaaaaaagaa gggttctatg
     2341 atatttggtg tccttactcg ccttgagtaa caacaccaaa tatcatagaa ccacttaatc
     2401 agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa tcatcggcaa
     2461 atagttaaag aaaaaactta atcgttgaaa gaagcccaat tgcttaaaga aggcaaactg
     2521 actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac taataactgt
     2581 taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa gcagaacaag
     2641 taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat tatggatcca
     2701 agtcgataga ttacaagagc tttcattcgt attgatactg aataagccag taaaaataca
     2761 atcacctatc ccatataaac caactgttgc aacaacaagt attgcaagtg tttttgatgt
     2821 tccaacaaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc cagatacaac
     2881 tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca cactaattac
     2941 ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg tcgataaatc
     3001 actcatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa tcattttctt
     3061 cccctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct cattttaaca
     3121 catattttta ttctagttgt cttttgaaca atcaataaca taaaggtgag tttcttctac
     3181 attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct cctactcaaa
     3241 aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
     3301 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
     3361 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
     3421 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
     3481 gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
     3541 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
     3601 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
     3661 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
     3721 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
     3781 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     3841 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     3901 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     3961 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     4021 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     4081 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     4141 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctagtt
     4201 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     4261 aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     4321 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     4381 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     4441 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     4501 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     4561 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     4621 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     4681 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     4741 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     4801 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     4861 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     4921 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     4981 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     5041 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     5101 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     5161 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     5221 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc atcaacccaa
     5281 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     5341 tctttctatt atactcattt gagaatcttt tcacaacctc gttttgttat tcttctctta
     5401 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     5461 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     5521 tagaaaaaat ctcactcctc tgattataaa agagggggca ttaacctcgt gtatactaaa
     5581 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     5641 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     5701 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     5761 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     5821 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     5881 tccaagccca gtctaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     5941 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttata ttgagttgat
     6001 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     6061 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcatttca cctaatgaat
     6121 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     6181 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     6241 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     6301 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     6361 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     6421 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     6481 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     6541 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     6601 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagatttctg
     6661 cctcagcaaa aacgtctaaa atatcatcag tggtcataga caaggcgatt tttaattttt
     6721 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     6781 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     6841 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     6901 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     6961 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     7021 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     7081 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgataa ggcgctgggt
     7141 atcatgagtg aatgatattg ctgaatacaa atagtccgac cacattaagt tgatcggact
     7201 tcataagcat tttattgact aatacttttt tgcttaagaa ttgattctaa catcttttgc
     7261 taaagttaag catcctatag ttcctgcatt atctgctaac ttaggtgtga caatgtattc
     7321 ttcgatattt ggcgtatcga catagccgtt aattagttct aaaaaacgtc tttgtacttt
     7381 ctttttcaga tgttcttgtt gcatgacacc accaccaaaa attataatat ctggtgaaag
     7441 catcagggtc gtattacagg cacattgtgc aatataatcc gcctcgattt cccagaacgg
     7501 atcttctata gaaagatctt gtcccttggt tcccgtacgc atctcgatag cgggtccaga
     7561 tgccaaccct tcgagacaat catgatgaaa aggacaagta ccagagaaag tatctcctga
     7621 atgtctctta actaacgtat gccccatttc tggatggcta aacccttcaa caaaaatccc
     7681 atcttgtata gctcctccac caattccagt tccaacagtg taataaacaa cactggataa
     7741 accttttcca tgacctgaaa catactcccc gtaacaagca gcattcacat cggttgtcca
     7801 ggcaattgga atttcgaaat gcttattcaa cgttccaaca aaatcaaaat ttttccaggc
     7861 agtttttgga gtagaggtga tatgtccata ggtctttgat tcacgatgga tatcaatcgg
     7921 tccaaatgac cctactccaa tgctttctaa ttgttcttta tagtgattaa agaaatcaat
     7981 taccaaactc attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt ctaaaacctt
     8041 taaatcatca ttgccaatgg cacaaacaaa tttagtccca cctgcttcta ttgagccata
     8101 catttcttac tccaccgtcc ttttatctag gaattcgttc ttccctgtct aatagttttt
     8161 cgaaaaactc atgtggttcc ttttggtacc aataggcaac agaagaaata tcgtcttgac
     8221 gctcataaag cttgatgtca tcatttccta gggtttggaa agtaacacgt aaattttctt
     8281 tgaaactaat ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt aaagcatcat
     8341 cgccaaaacc atgaactgga tttggattag gttttccagc actaaaataa tcccgggtgt
     8401 gatctctttt agattgaaaa ggatagccca taaatagcgt attaaaattt tgagcatatg
     8461 ccctttctcc aaagttcttt ttatggaaag cccaagcgcc gccaaaataa tcttctgctc
     8521 ctgtagatgt tattgtaggg aaatcttcat ctccatctat ataaaactta aattctcctt
     8581 ccccccacca gtatctttcc aatgcgcaca aagcaaagta ggttcctaca taatacccag
     8641 tgcctttgat tccatcaata agggtgtaat cttttccttt tgtcgtgatg ggttcacggt
     8701 tccatttagc atgaaaatac atcgcatcat ccggtacttc agtcaatgca taattaaatg
     8761 tataaaaaat gtgcttaata aactcaggat gttcattcgt taaagtaatc ttagcttttt
     8821 ttcgaaatgg catctcaaaa tagcaattca tccctgatgt tggattcact acgattggta
     8881 aggaattcac ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct aatggacttt
     8941 ctacagaagg agtggattca tcatcccaat aaatacgcaa aattaaatca cgtaagacga
     9001 aactcccagc gattgttttg tctgtcactg tcatccaaaa atgacgtaaa attccagggc
     9061 cttccatttc tgctaaagta attgtctctc cagcatttat cggaacagaa ggtgaccctt
     9121 ttctcccaat tcctagatga ctcgctgtca ttgccgcttt ccctttttca ccatttttat
     9181 tttctggtga aatagttcta ctttcctctc ccttgtggag ccatatatta tttaaacaat
     9241 tcattttatt cgtcctctat cctttcttca cttttttgat tattcatata gatgatttgt
     9301 tgttgtgcct ccgcaataat tgctccaata tctaaattat catttcctaa ctgcaaagcg
     9361 attgacattg ccaagggaag attcattcct gcaattacgt gcactctgtc agaaatatag
     9421 gtaaaaaact tttgatttac acttcctcca gccatgtcag ttaaaacaat tatttcgtct
     9481 gtagtatcca ctaactcttt aatttgttca tctatatcat caatttcttc gacatatgca
     9541 cataagctac taatttgatt atgcatacct gttaaaaact ctagtgtctc tttaaaaccc
     9601 tcagctagta ttgaatggct agcaatgatc aatcttctca taattcaaat ccttttcctt
     9661 tacgctaata ttccaaatgc agccataacc atcgcaaaaa caataacgag tagaatcaaa
     9721 atggtcatac tgattttttt cttactgagt agataatatg ctgcagcagt aataattact
     9781 ggaattaatg ttggtaaaat tttgtccaac attggttgga ttttcatact aacttcacca
     9841 aaagtgaatt taagtggcgt tgaaacactg ataactgaag caattaatga tccaactact
     9901 gttagcccca atactgaagc agcttcagtt aaataattaa tcttttcacc gtattcagta
     9961 acaatcttca ttccctgctt ataaccaaat tcaaagcctc tcattcggaa aaagaagatt
    10021 accatgatta aaatgaacca taaaaagatt cctacaggat tattttctaa agccatataa
    10081 gccgcaattg atcccatgat ggttgggatc atcgtcatca aaagagagtc cccaatacca
    10141 gcaattggac ccatcgtacc aactttaaag tcattaatcg catcttttgc ttctaatcct
    10201 tgcttttcct ccattgcaac acaagcacct aaaataatgg ctgccagcca aggctgtgta
    10261 ttgtaatact gaaagtgatt atttaaagct tcgatgtatt catcatcatc tttgtataat
    10321 tttcttaaaa taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt ttgataatta
    10381 aaggtacaaa ctgacatcaa ccaacgatag ttcgctttcc taagatcctt tttcgttact
    10441 tgatattttt cgctcatttt attcatcctc cccaataaac ccaacatcag aagctcccgc
    10501 agaagcagtg atttgtgcat ctttttcagc tctatgatac acattaaatg ctaaagcgaa
    10561 tcctatgatc gccgctccaa taataggtac cttcaaaaaa gcggctaaga aaaatccaac
    10621 aagtaaataa ctaatgtatc gacctgtagg cataaagcgt aacaacatcg caataccaac
    10681 agcaggcaac atttttccgg ctaatgttag cccatccata aaccaattag gtaaaacatc
    10741 caaaacgtaa ttaacagcag actgtcctaa agtaacggca ataaatactg gaattgctgc
    10801 agataaacca aagaaaattg gccccaacca caaaacacta ttcattttct taaattctcg
    10861 tttattcgca tattcctgtg atttgcgaac aacaaaacca tttaagattt tgacaattac
    10921 atctagttgc acccctaaca tacctacagg tagtcctatt gctaatccgg cttctactcc
    10981 aagctggcta ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg gtacagatga
    11041 gccaccaaaa ccggcaactc ctaatgacat caattgaact gtaccgccaa ctatcaaacc
    11101 tgtttttata tcacccatca aaattcctgt gaataatccc caaaaaactg cgtaatttac
    11161 aaagatcatc ggaatcccat aataatcaac atgtttaata aatgctaata tagtcagcat
    11221 aataatttga aaaatagtaa aatgcattat ccttactcct ttatcctaat tttgattgaa
    11281 tttcctgtgc cttatccgcc ggaacaaatt gtgtaacaat ttctacacca gaatccaaca
    11341 attttttata cgtgctgata ttttcatctg atgcataggc tctagaagat agctgtaatg
    11401 cattatcttt aacaacagta ccgccaacaa ttagtcggtc aattctaata ccgttatcta
    11461 ctaaatgaag gattgtttcc ggctctttca cgatgacaaa tactttttcc tgatcgtatt
    11521 tccccttttt aaaattcgca atcgctgttt cttcagtgat aattgataca gccattccag
    11581 caggtctagc taaacgcata cttgattttt ggatgtcgtt atttgcgaca gaatcatcga
    11641 tcaccatcac tctgtttgct ttagacattg gtgcccattg cgtgacaata atcccatgca
    11701 ataaacggtt atcaactctc gacataacaa tactcataaa tacttcctcc tttatctttc
    11761 attttcttac aagcaattac catgccaaaa caaaaagaac ttgataaatc aagctctttt
    11821 tacataatca taaacgtatt taacctcatg aatcgggatg tcgacactat aataatgttc
    11881 aacgacactg aaagattgct taaaatgtgt ctgaaatttt tgaaattcct cactcgtttc
    11941 attgaaataa atttcatctt ccaaagtatt ttgttttact aatcgttcaa ttaagcaact
    12001 aatgtgaaca tataatccga aacacgtatt attatcaaat gaaattccta gatcattttg
    12061 taagatgtat atagcatctg aaacccgttc taataattta gtcggattta atatggtcaa
    12121 ttgctccatt aaattcgtta gactaaaatt cttcagaata ttattgttca aaactttcag
    12181 ttcattatca ttaaagtgtc tattcaattg attggagaat ttacttatat tcattcctaa
    12241 aatcatatct tcaattgaaa agaattctac atcttctaga tgtggattta gtgttccaac
    12301 aataaaaaga atattgttct ctttacgaag ctccgataaa tagttttcat tcactaaaga
    12361 gtaatagtca cacgtttcta tgacaagatc agtatccctt ggaaagctat cttcaagtat
    12421 cttttttaat ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa tcatatcctt
    12481 cttaatattt gatgattcat aaatataatc cacattttga ttttctttta cttgttcaag
    12541 aatctgttta agcggtaaat ctaataatat ctgatttgca acatcaatag ccattcttgt
    12601 tgaaacattg tttagtagtc cgaatttttt ttgagcgtct agttccaaat ttagataaat
    12661 tttctccagt gaacccatat caacaagtaa aataatttct tcatatccgg tacactcttg
    12721 taagtatcta ttaattttgt ctgcaatctc actacttgaa gtcattagtg gcatatcaat
    12781 ggcgtcgtat atatatctct caacgatcct attagcagta cttgctatac aggttgccgt
    12841 tagatctcca tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt tactacacat
    12901 taacttaaat aaaataacca aaacattttt ttgaattttt gttaaataaa tatcccctat
    12961 ttgaataaaa cgattgacta attgaacttc tcgactacac caactattaa tatcattgat
    13021 gaaggaatat aatttatcgt ccgatgttaa ttggatttga taaatccaag aattcaaaaa
    13081 gatagacgtt atttgaagat aataatagtc aaattctatt tgaaaatctc tctctatttc
    13141 cttaacatac tgtctaaatc gaatctcttg ataggtggga tatgttaaag ctgaattatt
    13201 ttggatatca acattctttt gtctaaaata tctatcatat ttctcaaaaa gctgctgctt
    13261 atcagtgtct ttcttccacg tttgataaag atactctata agttcgttga tattaaaaac
    13321 ttcgtctact ttttccaaat catccatttg aatatatttt aattcattct gtcttttcat
    13381 aaccttcatg ccgttgaaat tcacccattg tggtaaatga tacatctgaa tataaagttc
    13441 ttccaaatca tctaagaaat aaaaggcatt ggcacaggtc aattgaatga tatttttcaa
    13501 attgccaaca ttttcactaa agcgatagtt taataaaaaa tctaataatt gttgagatat
    13561 cagtattttc tttgctagtc tttcttcttc tgatttaaaa aatatcttga ttaactgttt
    13621 tctttcataa agagaacgac tgtctaatgt tggaagtgtt agctttacag gtattcttct
    13681 caacaaagtt gataatagtt tatcctcagg tggttctgat gttgcaaata taatccagca
    13741 acttgatgtg taccagtttt cattatcacc catccgatga aatttcctcg tatccataaa
    13801 ctgaaaaagt ttctcctgac attcattgct caatgcatga atttcatcta aaaataagac
    13861 tccattatta gctaattctg ctaaaccctt tcttccatct tcggctcctg tatatgcacc
    13921 ttttacatgc ccaaaaaaat tggacaagaa aagttctgga ttattggcat attcactaca
    13981 attaactgaa atgaaagatg ccttctcttc tatataatta ttggccaaca aatccttata
    14041 cacataattt gctaaatagc tttttccagt tccagtctcg ccatgaagta aaatgggtaa
    14101 gccatttggc ggatagtaga ttgctgcttt aatttgagca attacatttc gcagacttcc
    14161 ttgacttccg ataacccttt ctattccaac cctaaaagag ctatcatacc atttttccgc
    14221 ttcctctgaa gagctaaaaa tttgttgatt tggaactttc tccggtaaag aaaagaagat
    14281 tactggacga gtattgattt ttccaaattc tccatctttt acgaattcat tcaaatattg
    14341 agagacaata ttacgactaa catttaattt ttcagatatt ttctgtgctg tattgttccc
    14401 ccatttattt ttaatctcag agttctctat gacataattg acaatgcgtt cttttaattc
    14461 ttccatcaca gcacctcctc gacactattc gaattttgcc gacagtattt ttccataaat
    14521 atgacttgct aaaaaggctt tgtttagatc attcaattgg aacgttgctt gttttgcttg
    14581 atccgctaag acgttgaaaa gatactctgg cgaaatcgca taccccaaaa tatcaacgat
    14641 tgttgcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat acaacatcgt
    14701 ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc
    14761 caacaaacgg tcggataaat atttatagaa aatcagcccc aataaataat ttttatattc
    14821 tgacgcatcc attttactac ggagattatc tgccgcacta aataacttct gatttaattc
    14881 tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt
    14941 agcatactca gttttagaaa aagcaaaaaa ataaagaaac ttattcttga taacaagcta
    15001 cgtgacgtat atccctaaca caaaaatagc catatccttt ggtttggata cggctatttg
    15061 atattactca ttcaattatc aaatcacagt aggtgtgaat ctcatcggga tcggcaatat
    15121 ctacttctga ttaggatcaa aggtgatttt ctcaataaag gtgtgataca aagctttaac
    15181 ttgcttcttc tcactctgga taagaaagca atccaaacta gtcaatatct gttggacatc
    15241 tttgacttga atcggttccc cttgttgttg aaagatccct aagatttcat tctctcgaat
    15301 ttgtaactgt cttcgttttt cagtcaattc atttaaacga tcttttaaca tcggaaacaa
    15361 ttcgggacta tcttccaaag cagcttcctg tttttgctct aatagttaga ttttgcgtac
    15421 cattttcttc ttttattttc cttcgattgc tgattttaat atagtctaaa ggaaatttat
    15481 atttattgta ttctttataa cttcaagaga acttcgtttg agtattactg tattttttta
    15541 attccataag aaaattagtt attacctgcc acctaatcag caaatctgaa cctgagtcaa
    15601 tataattaat attattttcc atctccaaca ttgtaagtac tgttggtgtt aatataggga
    15661 ctttgtacaa ttgtattttt ttgattttat catctgcaca aaggagattt tttaaatcta
    15721 atccttcttt tatttcaaaa gttgaatttt ttaaatctat aagagtaaaa ccaattcctt
    15781 catactgaat ttctgaaaat cgaatttttc tcccagttga tagatcaagg ggcagaaaac
    15841 cttttattac attgtgagtg tcaccatcat ttacaatgtc attaggaatt ttgattttag
    15901 aattctgtgg agcacctatt ggaagataat aattcattat aattttgtag tcttcatgag
    15961 gaaataaagt acttttagga ttgctaagat attctcgtac cgaatctaag aatggcttat
    16021 ccattgaatc ttcatcccca tttaaactaa aaaacattcc tacaattgct tttgcaactc
    16081 taagtgaatt cattttcttt attgaatgtg ttaaatcaga cttaccattc tttaaacttg
    16141 tattgttata aaggaaattt ttattgtctt ctgcaaatct aatatattcc tcaacatact
    16201 cactaccaaa aaaactatta catttactac ataacgtatt ggaatggcaa cagttttttt
    16261 gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt ccttgacaat
    16321 gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta gactgcctcg
    16381 ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaat
//
LOCUS       DACECS010000066        15687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.066, whole genome shotgun sequence.
ACCESSION   DACECS010000066 DACECS010000000
VERSION     DACECS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.066"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IWO31_002177"
     CDS             68..751
                     /locus_tag="IWO31_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0302575.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IWO31_002178"
     CDS             753..1589
                     /locus_tag="IWO31_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0302576.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IWO31_002179"
     CDS             1600..3354
                     /locus_tag="IWO31_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302577.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IWO31_002180"
     CDS             complement(3630..3971)
                     /locus_tag="IWO31_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0302578.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IWO31_002181"
     CDS             complement(4039..6210)
                     /locus_tag="IWO31_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0302579.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IWO31_002182"
     CDS             6421..7278
                     /locus_tag="IWO31_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0302580.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IWO31_002183"
     CDS             complement(7349..8206)
                     /locus_tag="IWO31_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0302581.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IWO31_002184"
     CDS             complement(8191..10893)
                     /locus_tag="IWO31_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0302582.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IWO31_002185"
     CDS             complement(10906..12195)
                     /locus_tag="IWO31_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0302583.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IWO31_002186"
     CDS             12345..13391
                     /locus_tag="IWO31_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0302584.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IWO31_002187"
     CDS             13393..15546
                     /locus_tag="IWO31_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0302585.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DACECS010000067        15308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.067, whole genome shotgun sequence.
ACCESSION   DACECS010000067 DACECS010000000
VERSION     DACECS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.067"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWO31_002188"
     CDS             278..787
                     /locus_tag="IWO31_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0302586.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWO31_002189"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWO31_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0302587.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWO31_002190"
     CDS             2322..3302
                     /locus_tag="IWO31_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0302588.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWO31_002191"
     CDS             3424..4842
                     /locus_tag="IWO31_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0302589.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWO31_002192"
     CDS             4913..6382
                     /locus_tag="IWO31_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0302590.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWO31_002193"
     CDS             6392..6859
                     /locus_tag="IWO31_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0302591.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWO31_002194"
     CDS             6895..7767
                     /locus_tag="IWO31_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0302592.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IWO31_002195"
     CDS             7848..10382
                     /locus_tag="IWO31_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0302593.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IWO31_002196"
     CDS             10538..11311
                     /locus_tag="IWO31_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0302594.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IWO31_002197"
     CDS             11304..12095
                     /locus_tag="IWO31_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0302595.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IWO31_002198"
     CDS             12105..12596
                     /locus_tag="IWO31_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0302596.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IWO31_002199"
     CDS             12614..13024
                     /locus_tag="IWO31_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0302597.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IWO31_002200"
     CDS             13028..14428
                     /locus_tag="IWO31_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0302598.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IWO31_002201"
     CDS             14451..14825
                     /locus_tag="IWO31_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0302599.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5314 a         2153 c         3061 g         4780 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag agtaagcgcc
    15241 ccatagaaca gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt
    15301 ctggaggt
//
LOCUS       DACECS010000068        15023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.068, whole genome shotgun sequence.
ACCESSION   DACECS010000068 DACECS010000000
VERSION     DACECS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.068"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            61..624
                     /locus_tag="IWO31_002202"
                     /pseudo
     CDS             61..624
                     /locus_tag="IWO31_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            789..1170
                     /locus_tag="IWO31_002203"
                     /pseudo
     CDS             789..1170
                     /locus_tag="IWO31_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1301..2251
                     /locus_tag="IWO31_002204"
     CDS             1301..2251
                     /locus_tag="IWO31_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0302600.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2769..3224
                     /locus_tag="IWO31_002205"
     CDS             2769..3224
                     /locus_tag="IWO31_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0302601.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            3535..3891
                     /locus_tag="IWO31_002206"
                     /pseudo
     CDS             3535..3891
                     /locus_tag="IWO31_002206"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3904..4314
                     /locus_tag="IWO31_002207"
                     /pseudo
     CDS             3904..4314
                     /locus_tag="IWO31_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4429..4593
                     /locus_tag="IWO31_002208"
     CDS             4429..4593
                     /locus_tag="IWO31_002208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302602.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4744..5723)
                     /locus_tag="IWO31_002209"
                     /pseudo
     CDS             complement(4744..5723)
                     /locus_tag="IWO31_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5728..6495)
                     /locus_tag="IWO31_002210"
     CDS             complement(5728..6495)
                     /locus_tag="IWO31_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0302603.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6985..7410
                     /gene="lacA"
                     /locus_tag="IWO31_002211"
     CDS             6985..7410
                     /gene="lacA"
                     /locus_tag="IWO31_002211"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0302604.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            7427..7942
                     /gene="lacB"
                     /locus_tag="IWO31_002212"
     CDS             7427..7942
                     /gene="lacB"
                     /locus_tag="IWO31_002212"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0302605.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7953..8885
                     /gene="lacC"
                     /locus_tag="IWO31_002213"
     CDS             7953..8885
                     /gene="lacC"
                     /locus_tag="IWO31_002213"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0302606.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8888..9868
                     /gene="lacD"
                     /locus_tag="IWO31_002214"
     CDS             8888..9868
                     /gene="lacD"
                     /locus_tag="IWO31_002214"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0302607.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9896..10213
                     /locus_tag="IWO31_002215"
     CDS             9896..10213
                     /locus_tag="IWO31_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0302608.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            10213..11919
                     /locus_tag="IWO31_002216"
     CDS             10213..11919
                     /locus_tag="IWO31_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0302609.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            12061..13467
                     /gene="lacG"
                     /locus_tag="IWO31_002217"
     CDS             12061..13467
                     /gene="lacG"
                     /locus_tag="IWO31_002217"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0302610.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            13490..13762
                     /locus_tag="IWO31_002218"
     CDS             13490..13762
                     /locus_tag="IWO31_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ0302611.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13783..14682
                     /locus_tag="IWO31_002219"
     CDS             13783..14682
                     /locus_tag="IWO31_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0302612.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4908 a         2490 c         2900 g         4725 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccataga
       61 ataaatgttt gtcatactac gatttatcgt tgggtgcaag agtacagcaa agtcctctat
      121 tatctttgga agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat
      181 atcaaaatta agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc
      241 ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga
      301 ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc
      361 tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg
      421 aagtatctta acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt
      481 tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata
      541 tataaaaaga accgaagaaa tggaacgctc ttcggctttt cggtgtctac tgaaatcaag
      601 gtattaatgg gaataacagc ctaagatatt tggagttcag agagggtgcg tttgattttc
      661 aaacttcgca acagaaccga tgacactatc gttcttcatc aacgtaagtt agatttgttg
      721 aaagagcaga aaaaaggatt tctacaaaaa atgtttgttt gatattttaa atagaaaaga
      781 gagataaagt gaagttttta aattttttga actataaata tttaaactgg gtattatatg
      841 gtattacatt attttttatt atttattact tttttaccga tgatgatatt tttttaattt
      901 tagcaatact ttttcttgtt tcatctaatt ttagtgataa cttaattgaa gttcttacag
      961 aaccagaaat tcggtctatg acggatgaag aacaacagat tattaaagct atgatttcgg
     1021 aaaataagaa agacgtagta gttatcaaaa agattagaga gtatacaaat ttaggtgttt
     1081 tacaagccaa aaaagtatat gaaaaaaaaa aaaagggtct ataattacaa attagataat
     1141 acattagaag ctggagacaa gattgtctag ataatgtgtg agataaaatt tttggcaggt
     1201 tatcatacat aagacaaaat gtttttaaaa ctgataacgt atttattcca caacaattac
     1261 gcatttattt ctgagaaaaa tataaagcag gaggattaca atgctgaaaa cttattcctt
     1321 aacaaaagat acattattag tttcgtcaga atcaaatatc aaaaattctt ctgttgttta
     1381 tgtttatggt gacgaacgag aatatatcga atcttctgaa cataaatatg actttaaaat
     1441 aggcaatata ctaacgtttg atgatcgagt tgcatatgac acgatgatag ataagaacga
     1501 tgaaaaaagt ttgctcgtaa gttttttgtt tcccgaggta catgaaggtg ggcatctaga
     1561 taccctgacg acatctgttg ttttcttggt atttaaaaat aagttgataa tttttacaaa
     1621 acaaaaatta aaattcattc ccgaactctt atccaatatg gttttacgtg atgtcaatca
     1681 tttcgtggag gaagtacttt taaagatcat gcaaaaagat ttctatatga tgcttaacga
     1741 ccttcgtgga gtcaaggaaa aaattgatga tttagactca gaaatatcaa cgtccggttc
     1801 actcaaacca acgtttggtg atttattgac tcttcaaaaa tacatgatag ctttgtcaac
     1861 aacttataaa gctaaccata aagcgcttga tttcattaaa aaaaatttca caactattga
     1921 cgatattgat tttagtacta aaaaaataat tgatgattta tacgatacaa gtgatacaat
     1981 ggatagaatc attttaagtt acagccaata tttggacaaa ttggaaaata tgataaacaa
     2041 tatgatttcg tatcagctaa acatgattat gaaaacccta accgagatat caattgtttt
     2101 gactattcca gcaattattt ttggcttttg gggaatcaat gtccatgtcc cgtttgaaaa
     2161 gtcttcttat ggtgttttag ccgtattgat tatctctgct atactaagtc taatttgctg
     2221 gcttttgatg cgacgcaaaa catatttgta aagtatttgt gataccctaa tatgatttaa
     2281 agcagaattt ctttgttgac tttttaaaag gctgattcct ttcaataatt taatagcttc
     2341 agcggctgct cagaagtcaa taaattggac tatttttatt cagttactta ggcaacattt
     2401 gagatacgat tgcgattata aaaattgata taccaaccaa tggctgcttg gaagactgac
     2461 atattagcct cgatatcatc gaataactaa atacagaaaa atacaataaa tattatcaaa
     2521 ctagggacta tactagaaaa catcagtaca ttccttaact cagggtctat aattagaaaa
     2581 tcgccccttt gaaaactgat taaaacagcc cccattatct agtgactaga taatgggggc
     2641 tgtttttttg ttaagtgata taataaacca taaaagaagt ttagaaatac ggtcctggtc
     2701 atcaataaat aaacaattta attaattatt tatttaatta tttaatttca gaaaggtata
     2761 catgaatcat ggtgcaacaa attgtcctac ccatcaaaga ctccagcgtc ttaaagatgg
     2821 tacaagatac acttctcgat agtttccgtg ctggtcgccg aaattacacc gtttttcaag
     2881 ttggtaaggc cacgctacta cgtgtgagtg atgtcatgac attaaaaaaa tcagatgtct
     2941 ataatccaga cggctctgtc aaaaatgcaa cctttattca tgataaaaag accggtaaag
     3001 caaacacgtt atatttaaag cctgttcagc aagacttgtt gcaatatcat gattggctgg
     3061 cccaagaaaa tatcaattct gagtggttat ttccctcgac agcccattat gatcttcata
     3121 tcactgagaa gcagtttcat aaggtgatgg cgcatgttgg tgatctctta ggaatcaact
     3181 tggaagacaa agagaaaaaa ttgcgagtca gaaaaccgtt ttgagatatg aacagcattt
     3241 gccttcacgc cttagcgatt ttaaataata gccttgaaag tgattatgga aaagccctct
     3301 acgctcaatg caaaattgac gtagaaacta tttttgatcg attgaaaggt gtatttggta
     3361 tgtgaagaac ccatgtcaga ggcaagaacg ttgcacctac tgacattgga atgatgctta
     3421 tgagcatgaa tctaaccaaa ttatcccttg aggctagaag aaaagcggaa gcttttcaac
     3481 acaaagcagc caaaaataaa aaacgcgacg aagcaatcac atttatgatt attcatcgcg
     3541 ttttttatat ttagagctag ttatttgcta gacccactat ttacagatga aacgtttatc
     3601 acctttggtt ttgggcattt agtggaatta gcagaaccag gtcattatga cgaaaagtgg
     3661 caaaattgga aacttgaatc tttgccgatt tttcctgatc gatacgattt tgaagttgca
     3721 aaagataagg gaaagcagtt taaaattgtt gcagaacttc tcaaaaaggc aaatacaatt
     3781 attgttgcaa cagatagcga cagagaaggt gaaaatatcg cctggtcgat tatccataat
     3841 gcaaatgcct tttcaaaaga taaaacattt aaaagactat ggatcaatag cttagaaaaa
     3901 gatgtgatcc gaagtggttt tcaaaatttg caaccaggaa tgaattacta tcccttttat
     3961 caagaagcgc aaacacgcca aattgccgat tggttgatcg gcatgaacgc aagccctttg
     4021 tatacgttaa atttacaaca gaagggcgta caaggtacat tttcactagg acgtgttcaa
     4081 acgcccacct tataccttat ttttcagcgc caggaagcca tagagaattt taaaaaagaa
     4141 ccttttttcg aggtggaagc taagtaaaac aagggtcatt taagggcgtt ctaagcccca
     4201 cacagcgttt taaaacccaa gaggagcttt ttagcttttg tttcttctaa acaagctaaa
     4261 ataggcaatc aagaggggat aattgctgat gttcaaacca aagagaagaa aacgaatagt
     4321 ccgagttcgt tttctttaag tagtttgcaa tcaaaagtca atcagcttta taaagcgaca
     4381 gcgagccaaa ctttaaaagc tatgcaagga ctgtatgaag caaaattatt gagttatcca
     4441 agaacagata caccatttat tacagagaac gaatttgctt atttaaaagc gaattttggc
     4501 aaatatagcg gttttttagg acttgatctt gaaatgttgt tatcattgat aatgatacgt
     4561 tcaaacaaca gcacccaaat tttgacgaat tagtgaaact ctatgaaaaa gacgtagtaa
     4621 aacatgcaac atcctattct aatcagctgg taaagcttaa ttaggttata taagcttgaa
     4681 aataaagggt ttgtccaata tggacaagcc tttttcttat gttttgccac agatttgcca
     4741 cacttattgt gtaagagtat caaggatagg tataatttga tactttgttt ttttagtgat
     4801 gtgcaaataa atttctgtta cacgactacc tctagaatgc cctacacggt cttgaattgc
     4861 ttctaggggt attccttact ctgctaagaa actaatatgc gtatgacgga atatatgggt
     4921 cgtaatatgt ttattaaaac taagtttatt atcaatatca attcctgctc gttttggatt
     4981 accttttcga tttaattttc cattttcagg tgagttgagg aaattgttaa ggacactata
     5041 atgaagaggt gttccattat agtcgggttt atgacgactt ttggaagtaa aaatccatcc
     5101 attatcttta taatcaggat tgaatctcat atgttggtga tttaactcaa tttcctcttt
     5161 aagaatttct atagcccttt tagataattg gacatttcgg taactttgta agttttttac
     5221 attaccttca tagctatttt ctgcagctcc tccgtgagtg gactgactaa tgatgatatt
     5281 ttgcttttca aaatattgat aggcaccaat attttggagt ttcgactctg taagtcaaca
     5341 gggccattag ttagaatacc tattgggatg cggtttttat gtagagccag cagtagttga
     5401 tgtatctcag gaatcatcgt aatctgatcg agttcatatt catagtcagc ttggaagtct
     5461 agagcttctg tcctagaaat actatcgatg ccaaattctt ccatagtctt ctgacagcga
     5521 aaaattctaa gctgctcaat actgatgagt tttttcgtgt atttaggaaa agcaacatca
     5581 gaccaatatc tgaaacgttt gtaaatgtta tctatttgtt ggatatcaat cgttggaaaa
     5641 catttctcaa cgctacgttt aaaggggagt tgatggtcat aaagagtgtc atcaaggtca
     5701 aaaacaatat ttttataagt cataattcta gtccgctttt aaaatagtag tgtactggct
     5761 aaattcctct aagtgttgag ggctaatctg gttatctgta atgatggtat cgagttgatc
     5821 tagattatag aagttaaaga aatcgtatcg atcgaattta gtactgtcta ctaataagaa
     5881 tttttctaca gcattgttta gggcaagttg ttgaatcaca ccttccttgt cactatatgt
     5941 agcaatagaa ttatgggtaa cagcatttgc actaacaaaa gctttggcaa atctaattgc
     6001 ttttaaattt gtcgaagcca ttgaacctac aaaagctcca gttatttctc tatattcacc
     6061 gccaagaagc aataaatcaa tggtttcgct atcatttaga atcaagaaca cagggagact
     6121 atttgtaatg acacgaattt tataaccttt acgacctttc aattctaatg ccagttgttc
     6181 aagtgtagtt ccaggtccaa taaaaatagt atcgccatct ttgattaaag agcaggcttt
     6241 tttagcaatg tcaatttttt cttttgtatt tagacttttc ttctcgatat gtgtcttttc
     6301 aagtggcttt ttagaggaaa gcaattgtgc accaccatga gtacgtgtta aaagtccatc
     6361 agcttctaga gcatcaaggt ctctacgggc tgtcatatcg gaaatatcta gttcatctat
     6421 tatttctttt atggttatgg tcccatcaat ctttaacata tctaaaattt tttctaatcg
     6481 tcgttttttg ttcatatgaa gacccttctt tcataaagta atttttttcc aaagataatt
     6541 ctcttttaat tgtatcataa aagacaatat tttcaaggta aaacaaacaa tttcaaacaa
     6601 aaacaaatgt tagatgatga aataagaaca gaggattgac gtatattagc ttaggtcaga
     6661 ttttgtataa gacgaaaata aagtaggacc tcttaatcag taagttatag aaagtaaaag
     6721 acttttgtaa tacttgaata gatatttcac gtccattttg tgatggatta aatgaacaaa
     6781 aatgaacaat aatttaacgg tgttatctat tttttaaaaa aacaaataaa aaaaacaaaa
     6841 aataaacaaa aaaagtgttg ctttttgttt gaatgcttga tattatataa acaaagaaat
     6901 gatgaaaacg ttttttcgaa cattttgcaa aatgttttct acttctacgt agcatttctt
     6961 tctaaaattt aggaggtggt caaaatggct attgttgttg gtgcagatct caaaggtacg
     7021 agattaaaag atgttgtgaa aaattttctt gtagaagaag gttttgaggt aattgatgtg
     7081 actaaggacg gacaagattt tgttgatgtt accttggcag ttgcctctga agtaaataaa
     7141 gatgagcaaa acttaggtat tgtaattgat gcttatggag ctggtccatt catggtagca
     7201 actaaaatta aaggtatggt tgcagcagaa gtttcagatg aacgctcagc ttatatgaca
     7261 cgtggacata acaattcacg aatgattacg gttggcgctg aaatcgtcgg tgatgagctt
     7321 gctaaaaaca tcgctaaagg tttcgtcaat ggtaaatatg atggcggacg tcatcaaatc
     7381 cgagtagata tgcttaacaa aatgtgttaa gaaaaggagt taaggaatga gaatcgcaat
     7441 tggatgtgac cacattgtca cagatgaaaa aatggcagta tcagaattct tgaaatcaaa
     7501 aggacatgag gtccttgatt tcgggacata tgaccacgca cgtactcact accctatcta
     7561 tggtaaaaaa gtcggtgaag cagtagtaag tggtcaagca gacttgggag tatgtatctg
     7621 tggtacaggt gtcggcatta acaatgcagt caataaagtt ccaggtgttc gctcagcgtt
     7681 ggttcgtgat atgacatcag ctatatatgc aaaagaagag ttaaatgcga atgttatcgg
     7741 tttcggtgga aagattactg gtggtcttct tatgaatgac atcattgaag ctttcattga
     7801 agctgagtac aaaccaacag aagaaaataa aaaattgatt gcaaaaatcg aacacgttga
     7861 aacacataat gcacatcaag cagatgaaga tttctttaca gaattccttg aaagatggga
     7921 ccgtggtgac taccacgatt aaggtataac aaatgattct gacagtcaca ctaaatcctt
     7981 cagtagacat ctcttatcct ctagaaacat tgaaaattga tacagtgaat agggtgaaag
     8041 atgttagtaa gacagctggt ggaaaaggct taaatgttac tagagtcttg tatgaatctg
     8101 gtgataaagt tactgctaca ggtttcttag gtggtaaaat tggtgaattt attgaaagcg
     8161 aattagaaca atctcctgtt agtccagctt tttataaaat ctcaggaaac actagaaact
     8221 gtattgctat cttacacgag ggtaaccaaa cagaaatttt ggaacaagga ccaacaattt
     8281 ctcatgaaga agcagaaggc tttcttgacc actatagcaa tcttattaaa caatcagaag
     8341 ttgtgacaat ttcaggaagt ttaccttcag gacttcccaa cgattattat gaaaaactta
     8401 tccaacttgc ttcggatgaa ggagtagcag tcgttttgga ttgttcaggt gcacctcttg
     8461 aaacagttct aaaatcgtcg gctaaaccta ctgccatcaa acctaataat gaggaacttt
     8521 cacagttgct tggaaaagaa gtaacaaaag atattgaaga actcaaagac gttcttaaag
     8581 agtcactttt tagtggtatc gaatggatag tggtctcatt aggtcgaaac ggtgcctttg
     8641 caaaacatgg tgatgttttc tataaggtag atattcctga tatcccagtt gttaatccag
     8701 tcggatcagg ggactcaacg gtagctggta ttgcatcagc tttaaatagt aaaaaaagtg
     8761 acgctgactt attaaaacat gcgatgacat tgggtatgtt aaatgctcaa gaaacaatga
     8821 cagggcatgt gaatatgact aattacgaaa cactaaactc acaaattgga gtaaaagagg
     8881 tataaaaatg gtacttacag aacagaaacg caaatctttg gaaaaaattt cagataaaaa
     8941 cggtgttatc tcagctttgg catttgacca acgtggtgct ttgaaacgtt tgatggcaca
     9001 gtatcaagat acagaaccaa ctgtagctca aatggaagaa ctaaaggttt tggttgctga
     9061 tgaacttaca aaatacgctt cttcaatgtt gcttgaccct gaatatggac ttccagcaac
     9121 aaaagcattg gataaagaag caggtcttct tcttgcctat gaaaaaacag gttatgatac
     9181 atcaagtaca aaacgtttgc ctgactgctt ggatgtttgg tctgcaaaac gtatcaaaga
     9241 gcaaggcgct gatgccgtta agttcttgct ttactatgat gtagacagct cagatgaact
     9301 taaccaacaa aaacaagctt atattgaacg tgttggttct gaatgtgtcg ctgaagatat
     9361 tcctttcttc ttggaaatcc ttgcttatga tgaagaaatc tcagatgcag gttctgttga
     9421 atatgcaaaa gtgaaacctc gcaaagtcat tgaagcgatg aaagttttct ctgatccacg
     9481 ctttaacatt gatgttctta aagtagaagt accagttaac gttaaatatg tcgaaggttt
     9541 tgctgatggt gaagtagttt acagtaaagc tgaagctgct gacttcttta aagcacaaga
     9601 agaagcgact aaccttccat acatctacct aagtgcaggt gtatctgcta aattgttcca
     9661 agaaacattg caatttgctc acgactcagg tgccaagttt aatggtgtgc tttgtggacg
     9721 tgctacttgg gcaggatctg tagaacctta catcaaagaa ggtgaaaaag cagcacgtga
     9781 atggttgcgt actactggat tcgaaaatat tgatgaactt aacaaagttc ttgttaaaac
     9841 agctagtcca tggactgata aagtatagta aaaacataaa acggaggata ttgttatgaa
     9901 cagagaagag atgactctct tagggtttga aattgttgct tatgctggag atgctcgctc
     9961 taaactttta gaagcgctta aagcggctga aaatggtgat ttcgctaagg cagatagtct
    10021 tgtagtagaa gcaggaagct gtattgcaga ggctcacaat tctcagacag ctatgttggc
    10081 tcgagaagct tctggggagg aacttccata cagcgttacc atgatgcatg gtcaggacca
    10141 cttaatgact actgtcttat taaaagatgt gattcatcac ctcatcgaac tttataaaag
    10201 aggagcaaaa taatgcataa actcattgaa cttattgaga aagggaaacc gttctttgag
    10261 aaaatctctc gaaacatcta tcttcgtgct attcgtgatg gatttatcgc tggtatgcca
    10321 gtcatccttt tctcatctat ctttatcctt attgcctatg tcccaaatgc ttggggattc
    10381 cactggtcaa aagacattga gaccttcttg atgactcctt atagctattc gatgggtatc
    10441 ctagcattct ttgttggtgg tactaccgct aaagctttga cggactcaat gaaccgtgac
    10501 ctacctgcga ccaatcagat taacttctta tctacgatgc tagcgtctat ggtagggttc
    10561 cttttgatgg cagctgagcc tgcaaaagaa ggaggcttct tgacagcctt cacgggaaca
    10621 aaaggtcttt tgacagcatt tattgcagcc tttgtgacag ttaatgttta caaggtctgt
    10681 gtgaaaaata atgttactat tcgtatgcct gaagaggttc caccaaatat ctctcaagta
    10741 tttaaagact tgattccatt cactgtagcg gttgttctcc tttatggatt tgaactcatt
    10801 gttaagggaa ctcttggtgt aaccgttgca gaatcaatcg gtacccttct tgctcctctc
    10861 ttctcagctg cagatggtta ccttggaatt acacttatct ttggtgctta tgccttcttc
    10921 tggtttgttg gtatccacgg tccttcaatt gtcgaaccag caatcgctgc tatcacttat
    10981 gccaatatcg atgccaactt gcatcttatc caagctggac aacatgcaga caaagttatc
    11041 acttctggta ctcaaatgtt tattgttacc atgggtggaa caggagctac attgattgtt
    11101 ccattcttgt tcatgtggat ttgtaaatca gaacgtaacc gtgccatcgg acgtgcctca
    11161 gttgttccaa cattctttgg ggttaatgag ccaatcttat ttggtgcacc aatcgtattg
    11221 aatccgattt tctttgtacc gttcattttc gctccgattg tcaacgtttg gatctttaaa
    11281 ttctttgttg acacattgaa catgaactca ttctctgcca accttccatg ggttactcct
    11341 ggtccgttgg gaattgtact cggtactaac ttccaagttc tatcatttat cttagccgga
    11401 ctcttggtag ttgttgatac tatcatttat tacccatttg ttaaggtata cgatgaacaa
    11461 attcttgaag aagaacgttc tggtaaaaca aacgatgctc ttaaagaaaa agtagctgca
    11521 aacttcaata ctgctaaagc agatgccgtt cttggaaaag caggcgttgc taaagaagat
    11581 gtagcagcaa acaataatat cacaaaagaa actaatgtcc ttgttctttg tgcaggtgga
    11641 ggtacaagtg gcttgcttgc caatgccttg aacaaagcag cagcagaata caatgttcca
    11701 gtcaaagcgg cagcgggtag ttacggtgct caccgtgaaa tgttgccaga gtttgacttg
    11761 gttatcttgg ctccacaggt cgcttcaaac ttcgatgata tgaaggctga aacggataaa
    11821 ttgggcatta aacttgcaaa aactgaagga gctcaatata tcaaattgac acgtgatgga
    11881 caaggtgctc ttgcatttgt tcaacaacag tttgattaat aaatttgtaa aagttatttg
    11941 agacgagatt attctttcaa ttccttataa tattgttaac catatcattg catttcattt
    12001 ccttggtttc cggaactcag aatatcgtct caaatgcttt ttttgaaagg atttatagtt
    12061 atgactaaaa cacttcctaa agattttatt tttggtggtg ctacagctgc ttatcaagca
    12121 gaaggtgcga ctcacacgga tggtaaaggc ccagttgcat gggataagta tcttgaagac
    12181 aattattggt acacagcaga gcctgcaagt gatttttacc ataaatatcc tgttgattta
    12241 gaattagctg aaaaatatgg cgttaatggt attcgtattt caattgcctg gtctcgtatt
    12301 ttcccaactg gctatggcga agtaaatgaa aaaggtgttg aattctacca taatcttttt
    12361 gccgagtgcc acaaacgtca tgttgagcca ttcgtgactt tgcaccactt tgacacacca
    12421 gaagttcttc actcagatgg agatttctta aaccgcgaaa atatagaaca ctttgtagat
    12481 tatgccgctt tctgttttga agagttccca gaagtaaact actggacaac tttcaatgaa
    12541 attggcccaa ttggagatgg tcaatacttg gttggtaaat tccctccagg cattcaatat
    12601 gatcttgcca aagtcttcca atcacaccac aatatgatgg tttctcatgc acgtgctgtg
    12661 aaattgtata aagataaagg ttataaaggc gaaattggtg tagtacatgc gttgccaacc
    12721 aaataccctt atgatccaga aaatccagca gatgttcgtg ccgctgagtt agaagatatc
    12781 atccataaca aatttatctt ggatgcaacc taccttggac actattcaga taaaacgatg
    12841 gaaggggtca accacatcct agctgaaaat ggtggagaac ttgatcttcg tgatgaagac
    12901 ttccaagcac ttgaagcagc taaagatttg aacgatttcc ttggtatcaa ctactacatg
    12961 agtgattgga tgcaagcctt tgatggtgag actgaaatca ttcacaatgg taagggtgaa
    13021 aaaggaagtt ctaagtacca gattaaaggt gttggtcgcc gaatcgcacc agattatgtg
    13081 cctagaacgg attgggactg gattatttat cctgaaggct tgtatgacca aatcatgcga
    13141 gtgaaaaatg attatccaaa ctacaagaag atttacatca ctgaaaacgg tcttggatac
    13201 aaagatgagt ttgtagataa taccgtttat gatgatggtc gtattgatta cgtgaagaaa
    13261 cacttggaag ttttatcaga cgcaattgcg gatggtgcaa atgttaaagg ttacttcatc
    13321 tggtcactta tggatgtctt ctcatggtca aacggatacg aaaaacgtta tggactattc
    13381 tatgtagact ttgacacgca agaacgctat cctaagaaat cagctcattg gtataagaaa
    13441 gtagcagaaa ctcaagtgat agagtaatat tacttgaaaa tgtaaaaaat tgttatccga
    13501 tactttagaa aaagtacaac acttatttag accaggcttt gaaaaggtat atattgttag
    13561 tttcgaagat tgtcctatga ttcctgagct tgaagctacc ccgttgctaa aatgtggtaa
    13621 atggtatgtt tcaactggaa aagagtggat ttgccattct gatttagagt tatcagcatt
    13681 tgaatgggaa tttttacaat cactcgatgt agaaatacgt gagactattc acttcgaggt
    13741 aaattatttg cctttccaat aataaaaaga aaggaattag ttgtgactat cgaattaaaa
    13801 aacgagtatc ttactgttca gtttaaaaca cttggtggtc aactgacttc gattaaagac
    13861 aaggatggga tagagtatct ctggcaagct gatccaaatt attggaatgg acaggcgcct
    13921 attctatttc ctatctgtgg tagtttacga aatgattggg ctatttatag acctcaagaa
    13981 agaccctttt ttacaggtct tattcgtaga catggttttg ttcgaaaaga agaatttatt
    14041 ctggaagaag tcaatgataa tagcgtgacc tttagtatta aaccaaatgc tgagatgctt
    14101 gataattacc tatatcagtt tgaactgaga gttgtttata ccttgaatgg taaatcaatt
    14161 agaaccgaat ttcaggtaac gaacttggaa atggaaaaaa caatgcctta ctttattgga
    14221 gcccatccag cattcaatag tcctttagta gaaggtgaaa actatgagga ttattatatt
    14281 gaatttagtg aggttgagtc ttgctcgata ccaaagagtt ttcctgagac aggattatta
    14341 gatttgcaag atcggacacc ttttttggaa aatcaaaaaa ttctcgattt ggactactca
    14401 cttttttccc atgatgccat tacacttgac cagctcaagt ctcgtagcgt aacgcttcgg
    14461 tcaaggaagt ctggtaaagg tcttcgagta gattttgatg attttccgaa cttgattctg
    14521 tggtctacaa ctaataaaag tccattcatt gctttggaac cttggagtgg tctctccact
    14581 tcacttgaag aaggtaattt cttagaagat aaaagacagg taacgaaaat ttcacctcaa
    14641 gaaacttcta gaaaaatcta tgatattact atccttaatt aaaatttgtt attatttgag
    14701 tcttcaataa gatttgaata ctcttacctt gtgatgatag attggtcttc tccgtgccag
    14761 tcttacatcg caaggttttt aagttttttg tgggtctatg tcaactgtag tgggtgacga
    14821 aaagctaata tctaaagaca gtctggtagt aaaaaataag attatttact acttcttgat
    14881 ttaaaataat agtggagcca ttaatttgtg gcaaatttaa tttaatattt tgcctcaaat
    14941 tagccacact ttttatagaa ctaagaggaa aaatgaaatt ttcttcctat taatatagtt
    15001 gtttgaaatc ttaaatatag cgt
//
LOCUS       DACECS010000069        14815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.069, whole genome shotgun sequence.
ACCESSION   DACECS010000069 DACECS010000000
VERSION     DACECS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.069"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(296..1024)
                     /locus_tag="IWO31_002220"
     CDS             complement(296..1024)
                     /locus_tag="IWO31_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302613.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IWO31_002221"
     CDS             complement(1002..2576)
                     /locus_tag="IWO31_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0302614.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IWO31_002222"
     CDS             2805..3743
                     /locus_tag="IWO31_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0302615.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IWO31_002223"
     CDS             3755..4666
                     /locus_tag="IWO31_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0302616.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IWO31_002224"
     CDS             4690..6162
                     /locus_tag="IWO31_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0302617.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IWO31_002225"
     CDS             6213..6824
                     /locus_tag="IWO31_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0302618.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IWO31_002226"
     CDS             6854..8767
                     /locus_tag="IWO31_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ0302619.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IWO31_002227"
     CDS             8895..11876
                     /locus_tag="IWO31_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ0302620.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IWO31_002228"
     CDS             11873..13534
                     /locus_tag="IWO31_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ0302621.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IWO31_002229"
     CDS             13574..14506
                     /locus_tag="IWO31_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0302622.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DACECS010000070        14525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.070, whole genome shotgun sequence.
ACCESSION   DACECS010000070 DACECS010000000
VERSION     DACECS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.070"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(330..692)
                     /locus_tag="IWO31_002230"
     CDS             complement(330..692)
                     /locus_tag="IWO31_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302623.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="IWO31_002231"
     CDS             complement(766..1146)
                     /locus_tag="IWO31_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0302624.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="IWO31_002232"
     CDS             1228..2403
                     /locus_tag="IWO31_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0302625.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="IWO31_002233"
     CDS             complement(2480..3064)
                     /locus_tag="IWO31_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0302626.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="IWO31_002234"
     CDS             3265..4263
                     /locus_tag="IWO31_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0302627.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="IWO31_002235"
     CDS             4375..4884
                     /locus_tag="IWO31_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0302628.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="IWO31_002236"
     CDS             complement(4914..5573)
                     /locus_tag="IWO31_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0302629.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="IWO31_002237"
     CDS             complement(5575..6339)
                     /locus_tag="IWO31_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0302630.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="IWO31_002238"
     CDS             complement(6347..7024)
                     /locus_tag="IWO31_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0302631.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="IWO31_002239"
     CDS             complement(7061..8446)
                     /locus_tag="IWO31_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0302632.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="IWO31_002240"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO31_002240"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="IWO31_002241"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="IWO31_002241"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0302633.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="IWO31_002242"
     CDS             9385..10242
                     /locus_tag="IWO31_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0302634.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="IWO31_002243"
     CDS             complement(10323..10658)
                     /locus_tag="IWO31_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302635.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="IWO31_002244"
     CDS             complement(10725..12095)
                     /locus_tag="IWO31_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0302636.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="IWO31_002245"
     CDS             complement(12173..12709)
                     /locus_tag="IWO31_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0302637.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="IWO31_002246"
     CDS             13018..14499
                     /locus_tag="IWO31_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0302638.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2891 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttatt
//
LOCUS       DACECS010000071        14497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.071, whole genome shotgun sequence.
ACCESSION   DACECS010000071 DACECS010000000
VERSION     DACECS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.071"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..560
                     /locus_tag="IWO31_002247"
     CDS             <1..560
                     /locus_tag="IWO31_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0302639.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="IWO31_002248"
     CDS             760..1074
                     /locus_tag="IWO31_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0302640.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="IWO31_002249"
     CDS             1095..1472
                     /locus_tag="IWO31_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0302641.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="IWO31_002250"
     CDS             1482..2255
                     /locus_tag="IWO31_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302642.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="IWO31_002251"
     CDS             2277..3680
                     /locus_tag="IWO31_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0302643.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="IWO31_002252"
     CDS             3862..5046
                     /locus_tag="IWO31_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0302644.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="IWO31_002253"
     CDS             5043..5333
                     /locus_tag="IWO31_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302645.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="IWO31_002254"
     CDS             5330..5551
                     /locus_tag="IWO31_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302646.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="IWO31_002255"
     CDS             5593..6372
                     /locus_tag="IWO31_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0302647.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="IWO31_002256"
     CDS             6439..6933
                     /locus_tag="IWO31_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0302648.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="IWO31_002257"
     CDS             6989..7627
                     /locus_tag="IWO31_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302649.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="IWO31_002258"
     CDS             7699..8094
                     /locus_tag="IWO31_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302650.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="IWO31_002259"
     CDS             8078..10531
                     /locus_tag="IWO31_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0302651.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="IWO31_002260"
     CDS             10528..12555
                     /locus_tag="IWO31_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302652.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="IWO31_002261"
     CDS             12552..13574
                     /locus_tag="IWO31_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0302653.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..>14497
                     /locus_tag="IWO31_002262"
     CDS             13591..>14497
                     /locus_tag="IWO31_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302654.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQ"
BASE COUNT         4925 a         2433 c         2876 g         4263 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaag
//
LOCUS       DACECS010000072        14391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.072, whole genome shotgun sequence.
ACCESSION   DACECS010000072 DACECS010000000
VERSION     DACECS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.072"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IWO31_002263"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IWO31_002263"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0302655.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IWO31_002264"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IWO31_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0302656.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IWO31_002265"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IWO31_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0302657.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IWO31_002266"
     CDS             2856..3731
                     /locus_tag="IWO31_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0302658.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWO31_002267"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWO31_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0302659.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IWO31_002268"
     CDS             4628..5062
                     /locus_tag="IWO31_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0302660.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IWO31_002269"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IWO31_002269"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0302661.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IWO31_002270"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IWO31_002270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0302662.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IWO31_002271"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IWO31_002271"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0302663.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IWO31_002272"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IWO31_002272"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0302664.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IWO31_002273"
     CDS             13333..14259
                     /locus_tag="IWO31_002273"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0302665.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         5048 a         2494 c         3124 g         3725 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt t
//
LOCUS       DACECS010000073        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.073, whole genome shotgun sequence.
ACCESSION   DACECS010000073 DACECS010000000
VERSION     DACECS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.073"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWO31_002274"
     CDS             66..767
                     /locus_tag="IWO31_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0302666.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWO31_002275"
     CDS             764..1885
                     /locus_tag="IWO31_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0302667.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWO31_002276"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWO31_002276"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0302668.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWO31_002277"
     CDS             3264..4172
                     /locus_tag="IWO31_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0302669.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWO31_002278"
     CDS             4207..4479
                     /locus_tag="IWO31_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0302670.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWO31_002279"
     CDS             4490..5536
                     /locus_tag="IWO31_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0302671.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IWO31_002280"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IWO31_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0302672.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IWO31_002281"
     CDS             complement(8547..9218)
                     /locus_tag="IWO31_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302673.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IWO31_002282"
     CDS             complement(9211..9705)
                     /locus_tag="IWO31_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0302674.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IWO31_002283"
     CDS             complement(9725..10945)
                     /locus_tag="IWO31_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302675.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IWO31_002284"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IWO31_002284"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0302676.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="IWO31_002285"
     CDS             complement(13021..14103)
                     /locus_tag="IWO31_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0302677.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DACECS010000074        14019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.074, whole genome shotgun sequence.
ACCESSION   DACECS010000074 DACECS010000000
VERSION     DACECS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.074"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..807)
                     /locus_tag="IWO31_002286"
     CDS             complement(151..807)
                     /locus_tag="IWO31_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0302678.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1000..1278
                     /locus_tag="IWO31_002287"
     CDS             1000..1278
                     /locus_tag="IWO31_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302679.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1297..2505
                     /locus_tag="IWO31_002288"
     CDS             1297..2505
                     /locus_tag="IWO31_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0302680.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2653..3018)
                     /locus_tag="IWO31_002289"
     CDS             complement(2653..3018)
                     /locus_tag="IWO31_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0302681.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3165..3938)
                     /locus_tag="IWO31_002290"
     CDS             complement(3165..3938)
                     /locus_tag="IWO31_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0302682.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4112..4972)
                     /locus_tag="IWO31_002291"
     CDS             complement(4112..4972)
                     /locus_tag="IWO31_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0302683.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4972..6390)
                     /locus_tag="IWO31_002292"
     CDS             complement(4972..6390)
                     /locus_tag="IWO31_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0302684.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIVIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6447..6896)
                     /locus_tag="IWO31_002293"
     CDS             complement(6447..6896)
                     /locus_tag="IWO31_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0302685.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7341..7604)
                     /locus_tag="IWO31_002294"
     CDS             complement(7341..7604)
                     /locus_tag="IWO31_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0302686.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7703..8998)
                     /locus_tag="IWO31_002295"
     CDS             complement(7703..8998)
                     /locus_tag="IWO31_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0302687.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8995..10266)
                     /locus_tag="IWO31_002296"
     CDS             complement(8995..10266)
                     /locus_tag="IWO31_002296"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0302688.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10431..11300)
                     /locus_tag="IWO31_002297"
     CDS             complement(10431..11300)
                     /locus_tag="IWO31_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0302689.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11592..13202)
                     /locus_tag="IWO31_002298"
     CDS             complement(11592..13202)
                     /locus_tag="IWO31_002298"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0302690.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3992 a         2955 c         2394 g         4678 t
ORIGIN      
        1 aaacggcagg ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag
       61 ctcaaaaaac ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc
      121 aaaacgctga tttcacaacc tctttgttca tcaaaagata atattgtctt ctaacaagtc
      181 catattcaaa ataacgatat gatggtcttt aacggtgata ataccatctt ttttcagttg
      241 ttttaaaatt ctgtttacac tgctagcagt tgagatgcca caaaatttgg cgatttcttc
      301 attcgtgatc gaaaaatcga tcagcatccc ttcttctgtc cgcacaccga ataaatcata
      361 taattcgtaa agttgcgtac aaacagctcc cagctttccg tttaatagca tttgctgcat
      421 ttttttcatc gaataaagca gacgatagcg gtaatactct tttacatagt gttgtagctt
      481 tgggtcttgg ttgatatttt gccaaaagcg cacacgattg attcgataaa actctgcata
      541 ttctgattca actcgaatat tatacggcga atcgatgaat tgtgaatatt cgtcttttaa
      601 aagagagacg atttcaagat ctttgatata gcgaaggtta aattctctcc catcatttga
      661 aataacactt gcttttaccg ttccctcttt caagatatac gtatgggaat cttccatccc
      721 ttcgaatgtc aaatatttat gatatccttt gcgtataatc ggaaaatcat ttttatccaa
      781 atattccttt aagactctac tatccaatga tgctccccca tccaaacttt catctaaaaa
      841 gatcatacag tactttaaaa ctttttaaag tccaaaggta ttttactaat gatattaaca
      901 tttgttaagt acctttttcc atcacttatg cgattttttt cctttttttt attcgttata
      961 ctgcagaagt aaaatttttt aagaatagag ggaaaaaaca tgaattactg gatgaacaca
     1021 atcattaatc gattagaaac agcatatcaa acaagatttg atatgaaagc ttctttagtc
     1081 tttttaaatg atgcgtatca aaactcgatc gaattgatca aagcagtgga tgagaatcca
     1141 acaaacgaat gcgaagaatt tttaaatctg tttatgtcta caagagattt atttattcgc
     1201 caacttgttg atcgctatcc atccaattat cacgatgtag aagttcaaat acaaaaatta
     1261 aaagcatatt cagcttaagg ggaatgtata acatacatgg aaaagaaatt atttaataaa
     1321 tattttattg gaatcacatt gatcaatttt attgtctacc tgatttatta tctgttgatg
     1381 gtcatcatgg cagtcattgc gcaagacacc ttacacgcca gcttgagcca agcaggtttt
     1441 gcttctggga tctatatcat cggaacattg ctggcacgct tattcatggg gaaacagtta
     1501 gaactatttg gtcggaagtt taccttaaga ggcggcgcga tcttctatct cctttcaaca
     1561 ttagcatatc tttatacgcc aactatcggg gcattgtatt tagttcgatt cttgaatgga
     1621 ttcggctacg gtacagtttc tacagctaca aatgctatcg ttactgcata tatccctgca
     1681 tctaaaaaag gggaaggcat caattattat ggattaagta ctagtttagc tgccgctgtt
     1741 gggccatttt taggaatgat tttgctgaat atgactgatt ttcgtttcat tatctggttt
     1801 tcaattgtac tggtcttctt tgttacgatt gcctgccttg tctttcctgt aaaaaatatt
     1861 ccattatctg aagaacaaaa aatatctctt cgctcatgga atatcaatag ttttattgaa
     1921 aagaaagcac ttttcattac aggaattgcc tttttgatgg gtctttctta ttcaagtgtt
     1981 ttatcatttt tatcttctta tacaaaagta atccacttag ttgctgctgg gtcatttttc
     2041 ttcgtcgtat atgctttggt catcacattt acacgtccat tgactggaag gattttcgat
     2101 gtcaaaggag aacaatacgt gatgtatcct agctatttat tcttaacagc aggattattt
     2161 ttactgagtg taacgacgaa tagtgtgaca ttgcttatct ctggagcatt agtcggccta
     2221 ggttatggta cgtttatgtc aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat
     2281 cgaatcagta ttgctttatc tacctacttt attggtttag acctaggctt aggcgttggt
     2341 ccttatttga tggggatgct aaaatctctt acttcattta gaggattgta tgtgattgcc
     2401 ggagtcattc cattgatatg tacagcactc tatcttgtta atggccgaaa acatagtcat
     2461 gaagcagagg gcgaattgat tgaatcattg aaaaatgaaa tttgatcttg atgaatagat
     2521 aaaaaaaacc gttcagatct gaaaataatc tgagcggttt tttgagtaaa aatagtttat
     2581 gattattctt aattaaacat ctatttattt ttaaagcttt catttatcga taagaatagt
     2641 ttaactgttg atttattcca ttttccgttc atctggtacg atgccttgac ttagcaacca
     2701 cttgtggaag aaccattctg ccacaccgat caatagagca ggaatcaata ctttcatcca
     2761 aatatcgact gtatcattca taaaatatag atagagccac aagataactg cagctgaacc
     2821 agcatctgcg atcgtcgctg cgatgttccc tactttacgt aagatcaaca gatcccctag
     2881 cacatagatc atgactacaa taattacccc aacaatcgtt gaatccataa agctaacacc
     2941 gtataaacct gtcagcacga cccacagaat cgcccagatc atgattgcct tcactaccaa
     3001 cgccttcaca tgtttcattt tctgcactcc tttctcctgg ataaaaaagt aagattatct
     3061 aacaagataa ccttttcatt attataccat cggctgagag aagcgagcaa agataaggtc
     3121 ctatctaaaa atgaggaagc cacttagaac attcacgtaa taatttatcc gtacagacat
     3181 tcgatatggt gttcctttac tttcgcatac tgttcttttg tcagttcttt acttgtcacc
     3241 agataatcga tttcatgaaa agcaatggct tgatgcatga atttatgtgc aattttttgc
     3301 tgtgtaacta aaacggcaag ccggtctgat tgttttctaa aagctttttt gaccattgcc
     3361 tctccttcat gagtttctgt tactccttgg ctgaaatcta gcccgcgaca actcattatc
     3421 gctaaatccg cataatatga caatgcatcg ttataggctt ttgaaccgtt gattgttgct
     3481 cgtttcggaa caattgcccc tcccaataca gatacttttg ccgttgtata ttcactcaac
     3541 atagcagctg tgacgatgcc attcgtaatg atctgcaaac catcaaatct tgctaggttt
     3601 ctgactaagt gtaaagaggt tgtactggaa tccaagaata agaccatatc atctcgaatc
     3661 aaagaagaag ctgtatctgc aatcttacgt ttttctgttt cttcttctaa tttggtgatc
     3721 ttcatcaggt aaggcacgtc aatttttcga ttgtcaataa gcatggcgcc accgcgaact
     3781 ctacggatca atccactttt ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta
     3841 taatttacta actcgatcaa atcggtgact cttgcaaatt ggttgttttt tagataattc
     3901 acaatctcct gctgtctatt ctccacttca ttcaacataa aatcacccca tttactttat
     3961 atagtaacag gaaaaaagag gttgtgacag aagtgattgg cttcgagaaa taagaagaaa
     4021 tttttcgaaa aaactacttc tgaacatcgt gtgtggttaa gagggtatga caagactatt
     4081 gtcataccct cttgaccaca catttttatt tttagtataa actagccttg cctactgtgt
     4141 tcaaaatttc cattttatgt ttgaccactt cttgtacctt tttattagct gctggataaa
     4201 ttgcattcgg ctcgtacatc gatggatttt cactcaatat ttctctcaaa gcattgaaaa
     4261 agacgctttt aagatctgag ctcaaattca ctttggcaac tccgtatttt actgcttcgc
     4321 tgacttctgc gtccggatta cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt
     4381 ggttcaattc tttcagtaag tcgatattta attttggagt tctgtttttc ggatacaagc
     4441 catgcgctgt tccaattgct acagcaagtg tatcgattcc tgtttctttc acaaaaatct
     4501 tcgcttgatc aggatctgta taaatgattg tttcagcacc tccttcagca gaaccattat
     4561 tcccaatcgt tccaagctca gcctccactg agacatttac cgcatgagct aacgcaacca
     4621 cttttttcgt aatctgtaca ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg
     4681 aagaatagcc gtttcgaatc gcccgtaaga catcttctat cgtaccgcca tgatccaagt
     4741 ggataacgat cggcagttta cttgtataag cataggctct tactgccgca acaaactcat
     4801 cacttaaata ggcgatttca tcaggatgaa tttctaagat tactggtgct tctaatctct
     4861 ctgcttcttc gatgattgct ttcatcatat catagctgca aatattgaag gctggtactg
     4921 caaattgatt ctctttagcc actttcaata actctttcat tgtatataac attacgcttc
     4981 cacctcattt gttagatttt catttttcgt ccacttgtct tgttttttct ctttcactgt
     5041 ttttttcaat agtccaagca taaaggctgt agtgactgta cctgatgcaa ttgccaagac
     5101 atacataggt aaattgttca taacatttgg aattagcatc acccaaatcc ctccatgagg
     5161 tgccattgaa gtaactccta ctgccatgga aatccctgca gcgactgctg aacctgtcat
     5221 caaactaggt atcacgcgga tcgggtcaga taccgcaaac ggtatagcac cttctgtaat
     5281 atacgaagcg cctaaaatcc agcatgattg acctgaagtc cgttcttctt tggtaaattt
     5341 atgtttgaat aataaagtgg ctaaagcaat acctagggga ggtgtcattc ctgcagccat
     5401 acaagcagca atcggtgcat ttactccagc tgacatcaaa ccgatcgaga aagtcgaagt
     5461 cgatttattg atcggcccac ccatatcagc tgccatcatg atcacgataa gtaatccgaa
     5521 caatagcccg gatgtatttc ccaagctatt cagccaattt gtcaaagcat ccaacaccca
     5581 tgtgatcggt tgttcaatca caaataccat caaaagccca ataattaaga tcgataaaaa
     5641 ggggacgaca atcagctgat aaatagcaga aaattctttt tttacgctaa ttttatttgc
     5701 cagaaaatca cagatatacc ctgctagcac gccaccaatc attccgccaa gaaaacctga
     5761 accacctgta gaggccagta atccggcaac aattcctggg gcaaaacctg cttttccagc
     5821 aatcgatgta gcaatccccg cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc
     5881 tccgatcctt gaaaatgcag ctgctaacac attatagtct gctgagttgg gatcagccgc
     5941 tgtaatccca aaggcaaaac tcagtgcgat gatgattccg cctgcgatca caaatggaag
     6001 catgtaattc acaccattca tgaaatgatt atagattgtc ggctttttct catttgcagc
     6061 tggttctttt tcattagaca caacgaatac gccaatatct ctcaatgctt cgtctattac
     6121 tacttgcggt tctttgattg ctcggcttgt agagacacgc tttacttttt tcccggcaaa
     6181 tcggtccaat tcgatttctt tatctactgc aagaatgatt gcatctgcct ctttgatttc
     6241 ttgttcagat aactgatttt ctgtttttac accttgtgtt tcgaattttg cttgatggtt
     6301 tagatttctt gctgttactt ctaatttttc tgccgacata taagtatgag caattccagt
     6361 cgggcaagca gttacaccaa cgattttcat gattattttc ctcctttttc ttcactatcg
     6421 atactcggac aaagactcta ctattttcat tcttgactaa tctgtctgat gatatctgct
     6481 ggcgtagtgg cttcttttat tccctttcta aaatctgcat tcatcaatcg tctggagagt
     6541 gtgctgagaa tctctagatg catatctgct gatttttcag gaactacgat ggcaaaaata
     6601 tattcaattg gcatttcatc tatcccgcta tagtccgtta atttggctgt atgaaaaaaa
     6661 cacagtgctg gttttgtcac atatttgctt tttgcatggg gaatcccaaa tccatcttct
     6721 attcctgtgg agatctcctc ctctcgtttg aatagtgcct taacgacttt ttgaacattt
     6781 cttaccttat ttgttttttt caacacatcc cctattcgct caaataatga tccttttgaa
     6841 tctatcgatg gatcgaaaac aatcagttct tctgcgatca attgatcaaa cctcataaaa
     6901 aacctctccc ccacaaaaaa tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc
     6961 tagctgtacc gttcaatatt atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat
     7021 aagtcattaa aatgaacgat ctagctcaca aacaatcgca agtctataac aaaacaaaaa
     7081 gccttccttg aaaggaaagc ttagcttcta attttaggta aatggttttt tacttcgaaa
     7141 taacccctct tcatttcgtt aatataaggt gtttttatca agtggataat taaaactata
     7201 tactagtttt tcattgtttt gtgcattatg atcctacgac tttttcagca tcaaacgaag
     7261 ttttaaattg gcaaaaaaca taaaaaaaca gactatccct atcagatagt ctgctaaacg
     7321 ttgagttatc agcgttttta ttattccgca gcgtttgcgt ctttgagacc gtattttttg
     7381 ttgaaacggt ccacacgtcc gtctgcttgt gtgaattttt gacgtccagt gtagaatgga
     7441 tgtgagtcag aagttacttc gacacggatg actgggtatg tattaccgtc ttcccattca
     7501 actgtttctt gtgaagtttt tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc
     7561 atgaatacta ctgggtggta atctggatgg atgttttctt tcatgatttt ttcgctcctt
     7621 tgccctgctc catttgctga accagagttg atttgtcgat ttacaacatt aacatcttaa
     7681 catgtttcag tcaaggttgc aattatcttt tcagatttct ggtcggattt tttttaccaa
     7741 aggagacatc ttgaaattct ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc
     7801 ggatcagttg ttccgtatat tctaatgaat ctccagtcat attttttcga agtttccaaa
     7861 tttcttctaa ttcttcactt ggcatcaata attcttcttt tcgggtacct gattttttga
     7921 tatcgatggc tgggaagatc cgacgttcag ataattcacg tgaaagttgt aattctagat
     7981 ttccagttcc tttgaattct tcatagatca cgtcatccat acggcttcct gtatcgacta
     8041 aagcggtcgc taaaatcgtc aaactgccac cttcttcgat gtttcttgct gccccgaaaa
     8101 atttcttagg tttatacaat gcggctgggt caatcccgcc actcaatgta cgtccgcttg
     8161 gtggaacgac taagttatag gcccgagcaa gtctagtaat actatccatc aagataacga
     8221 catctcgttt atcttcgact aaacgcattg cgcgttccaa tactaattca gaaactcgag
     8281 tatggttttg cggctgcaga tcaaatgtag aagataccac gtcgcctttc acgcttcgtt
     8341 ctagatccgt tacttcttcc ggtcgttcat caatcagtag aacgattaat tctacttccg
     8401 gatagttttc ggtgatacca tttgcgattt cttttaatac agaagttttc cctgcttttg
     8461 gcggtgcgac gatcaatcca cgctgaccaa aaccgattgg agaaaagata tcgatcattc
     8521 gggtagatag tctttgcggt gttgtttcca gttgaagctg tctttgcgga taaagaggtg
     8581 ttaaggctgg aaaatgcgga cgttcttttg cttcttccgg atcttttcca ttaacgcttt
     8641 ccacgtgcat caaaccataa taacgttccg attctttagg tggacgggct tttcctgcca
     8701 ctttgtcccc atttctcaaa ctgaatcgtc tgatttgtga ggcagaaata tagatatctt
     8761 ctgcacttgg tccataattg attggccgta aaaaaccgta accatcttga ccaacgatat
     8821 ccaatattcc ttccatataa taaaatcctt gtttctctgc ttgtgcacga atcacagcta
     8881 gagataattc ctttttattc atcttactgt aataaggaat tttaaattct ttggcataac
     8941 tataaacttc ttttaacgtt ttgttctcta gttctgccat tgtcaaataa tcgctcattc
     9001 agctcccact tcctctatca tttcaattga tgcccctaag gctgttagtt tctcgataat
     9061 atgatcataa ccgcgtaaga tataatctac attgtaaatc gtcgttgtcc cttctgccaa
     9121 tagtccagca atcactaaac aagcacctgc ccgaagatca gaagccacga cttctgtgcc
     9181 gtgaagttta tttggaccat tcacgatgat catatttcct tccacggtaa tatcagcgcc
     9241 cattcgtgct agttccggca catggttcac acgtttcgca tagatcgtat cgatgatttc
     9301 tgaagtgcct gtagtcatca atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa
     9361 tcctggataa ggataggtca tgatgtttgc gggttttaat tctttcgagg ggtaaacttc
     9421 gatttcatct tcgctgattt tcatatggac accaatttcc tgcaattttg ctataaagct
     9481 ttccaaatgt tcaaagatca catttttcac tttgatacca tttcctactg cagctgctaa
     9541 tgctagatag gttcctgctt cgatgcggtc aggaatcatg aaatgccgac agccatgtaa
     9601 tgtttcgact ccttcgatac ggatcacatc tgtacccgct cctcgaactt ttgcccccat
     9661 attattcagt aaagttgcca catctatgat ttccggttct cgtgcagcgt tttcaattac
     9721 tgtctgtcct tttgctttta cagcagctaa catcacattg atcgttgctc cgactgaaac
     9781 cacgtccatg aaaatccgat tgccacgcaa tcctttattt tctgtccgta aatacatggc
     9841 accatgttca tttgtcactt cagcacctag agcttcaaat cctttgacgt gtaggtcaat
     9901 cggtctaggg cctaaataac agccacctgg caggcctact accgcttcgc caaatttacc
     9961 aagcagtgtt cccataaaat aataagaagc acgcaaacta ttgatttttc cgtgcggcat
    10021 cggaatcgat acgacttctc gaggatcgat ttctagtatg ttgttggaaa aatggacagt
    10081 tgcgcccatg atctctaaaa tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc
    10141 ttccaagatc actggggaat cagctaagat tgcagctgga atcaatgcga ctgcactatt
    10201 ttttgcaccg ctgatcgtta cttcaccagc taaaggtcgg tttcctttga ttacgatttt
    10261 tttcatctgt ttccaccttg tttattaatc ttttttcatg gatatgcttg tattttatca
    10321 tagataaaaa aaagaaacga gcatggtctg ccgttttaag taaaaaagaa tggagagcga
    10381 cttcaaaagt cgttctccat tcctatatat cgtatctgtt atgtggatgc ttacgctttg
    10441 ttagctgaac cgaaccagtc gatatgagat ttagcatctt caacgattgc tgctttacct
    10501 ggagctaata atttacgagg gtcgaatcct ttaccttctt tatctttacc agcttcgata
    10561 tatttacgag tagctgcagc aaatgataat tggaattcag tgttaacatt tacttttgag
    10621 atacccattg agatagcttt ttcgatttga tcttgaggaa tacctgatcc gccatgtaat
    10681 actaaaggca tgtctggacc tacagcatca gcaattgctt gtaagtggtc aaaagctaat
    10741 cctttccagt tttctggata ttgaccatgg atgttaccga taccgcaagc taagtagtcg
    10801 atacctgtag caaccatttg tttgcattct tcaatatcag ctaattcgcc gttaccgatg
    10861 attccgtctt cttctccgcc gattgaacca acttcacatt caactgagat accttttgca
    10921 tgtgcttttt caacaacgtc tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa
    10981 ccatcgaaca ttactgaagt atagcctaat tcgatacatt ccaaagcagc ttcgtaatca
    11041 ccatggtcta agtggatagc aactggaaca gtgatgttca ttgcttcaac taaatcagtg
    11101 attaaatctt tacagatttt gtaaccaccc atgtatttag ctgcacccat tgatgtttgg
    11161 ataagtactg gtgcttgttt tgcttcagcc gcttcaagga ttgcttgagt ccattccaag
    11221 ttgtttgtgt tgaatccgcc gacagcgtaa ccgcctttac gagcagcttt taaaaattca
    11281 gctcctgata ctactggcat aataaaattc ctcctaagtt ttgtaaaact aaatttgtta
    11341 agacaattct taaccaacga ccctatttta ccaaacattt cggtagtttt ctactaaaat
    11401 caatgaaaat gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt
    11461 taatcgaaaa aagtcttttg ctttctatct tttttcctac aaataataac aaatcagaaa
    11521 aagagaaaag tgacaagaaa attgcttatc cccttctctt gctacttttc tctttctatc
    11581 aagttcaaaa attattttgt ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt
    11641 aatttttgcg ggcgatttgg acgagaaatc aattctgggt ggaattggca tcccacaaag
    11701 aattttttct ctggcagctc tacgatttct actaaacggt tatctggtga gacacctgaa
    11761 aagactagcc cttgttcttc aaacaattga cgatatttgt tattgaactc atagcggtga
    11821 cggtgacgtt cttgcacaac atcggctcca tcatatgctt cagccgtctt cgttcctttt
    11881 ttgattttac atgggtataa tccaaggcgt aaagttccac ccaaattctc gatgttttcc
    11941 tgatcagcca ttaaatcaat aatattgttt gctgtatctg gattcgtttc agctgatgac
    12001 gcatcttcta atccaacgac gttacgtgca aattcgacac aagccatctg cataccaaga
    12061 caaataccta aaaacggaac gtcattttcg cgagcataac gaatcgcttc gattttccct
    12121 tctactccgc gatcaccaaa tccaccagga accaagattc catctgcgtc tttcaaacgt
    12181 tcttctacat tttctctcgt taattcttgt gctttgatcc aatcgatctc gatgtctgaa
    12241 ttataggcga aaccagaatg tttcaatgct tcaactactg agatatatgc atctggtaac
    12301 tcgacatatt taccgacaag cgcaatccgt acttttttct tcaagttcaa gacacgttct
    12361 tctaattctt tccattctgt catgtctgct tctggtgtat ctagttttaa atggtcgcaa
    12421 acaatttgat ccattccttg tgcttgtaaa actaatggga tggagtaaag tgtatctaca
    12481 tctggcgact cgataactgc ttctggttcg acatcacaaa attgtgccaa tttgtttttc
    12541 acgctttgag aaacaggttg ctctgtacgt accaccaaaa tattcggttg gatacctaaa
    12601 ccacgtagct cttttacact gtgctgcgtt ggtttagttt tcatttctcc agcagctttc
    12661 aagtaaggaa tcaatgttgt atggatatac ataacattat ctgtaccgac atctgctttc
    12721 atttgacgaa gtgcttcaag gaatggcaaa gattctatat cccctactgt tccccctact
    12781 tctgtaatga tcacatctga atcagtcatt ttagctgctc gcatgatttt ttctttgatc
    12841 tcatttgtaa tatgaggaat aacttgaaca gttgctccca agtattcgcc cttacgttct
    12901 ttacgcaaaa cttcagaata gatttttcct gtcgtcacgt tggagtattt gttcaaattg
    12961 atgtcaataa aacgttcgta gtggcccaga tccaaatctg tttcagcacc atcatctgtt
    13021 acgaatactt ctccgtgctg atatgggctc atcgtccccg gatccacgtt gatgtatgga
    13081 tcaaattttt ggatagttac ttttaagccg cggtttttca ataagcgtcc taaagaagct
    13141 gcaacaatcc cttttccgat tgatgaaacg acaccgccgg tcacaaaaat atattttgtc
    13201 ataagttcaa aaactccttt ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag
    13261 ctccctattc ttcaataaga atagggagcc gaaatttctg taaacttctg accctctaaa
    13321 agggtgccca aggtgtataa tacaagctga ctaagggaaa gtcaagtact caaatgaata
    13381 ctatccgctt acaatttttt ctttttcgat atcctttgag aaattatttc ttaagtcctt
    13441 tatgagtgca aaaaaaattt aacccacata ataagcttga tataaacata gctaacgtta
    13501 aacctaagca aatcagaaaa tcgttactga aaaattttaa gtgaaataat agataaaaaa
    13561 ataaaacaaa cattatgata gataatatcc cacctaacaa agaactactt tttcctactt
    13621 gcagcaacag tttatatagc ttatcgtaga aacaatcttt ttctttcacc cttatttacc
    13681 tcctttacgt aaaaaacgta gtccacagtt ctcggatttt tggcatatct tttctattaa
    13741 ataatatcaa aaacaaaaca attccactaa ttttttcaaa gatgaccttt ttttcaacaa
    13801 aagtgccttg tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg
    13861 cgacaaaagt cgtgcctcct tatttctaaa taaacggtgt tctcctgcca actcctcggt
    13921 cacaagtcac aatatccatc aaacatgaga agctgttttt gaaattattt cttgtgattc
    13981 ggagttaaat ggtaggctca caacctctgg ataaacggt
//
LOCUS       DACECS010000075        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.075, whole genome shotgun sequence.
ACCESSION   DACECS010000075 DACECS010000000
VERSION     DACECS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.075"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWO31_002299"
     CDS             131..505
                     /locus_tag="IWO31_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0302691.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWO31_002300"
     CDS             complement(619..1248)
                     /locus_tag="IWO31_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0302692.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWO31_002301"
     tRNA            complement(1382..1454)
                     /locus_tag="IWO31_002301"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWO31_002302"
     tRNA            1668..1741
                     /locus_tag="IWO31_002302"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWO31_002303"
     CDS             1696..1845
                     /locus_tag="IWO31_002303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302693.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWO31_002304"
     CDS             2338..3666
                     /locus_tag="IWO31_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0302694.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWO31_002305"
     CDS             3868..4701
                     /locus_tag="IWO31_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0302695.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWO31_002306"
     CDS             4717..5454
                     /locus_tag="IWO31_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0302696.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IWO31_002307"
     CDS             complement(5609..6577)
                     /locus_tag="IWO31_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0302697.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IWO31_002308"
     CDS             6809..7642
                     /locus_tag="IWO31_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0302698.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IWO31_002309"
     CDS             7676..8515
                     /locus_tag="IWO31_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0302699.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IWO31_002310"
     CDS             8541..9101
                     /locus_tag="IWO31_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0302700.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IWO31_002311"
     CDS             9300..11021
                     /locus_tag="IWO31_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0302701.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IWO31_002312"
     CDS             11186..12328
                     /locus_tag="IWO31_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0302702.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IWO31_002313"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IWO31_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0302703.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACECS010000076        13734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.076, whole genome shotgun sequence.
ACCESSION   DACECS010000076 DACECS010000000
VERSION     DACECS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.076"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(143..1345)
                     /locus_tag="IWO31_002314"
     CDS             complement(143..1345)
                     /locus_tag="IWO31_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0302704.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IWO31_002315"
     CDS             complement(1348..1542)
                     /locus_tag="IWO31_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0302705.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IWO31_002316"
     CDS             complement(1589..2437)
                     /locus_tag="IWO31_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAQ0302706.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IWO31_002317"
     CDS             complement(2642..2992)
                     /locus_tag="IWO31_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302707.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IWO31_002318"
     CDS             3587..4033
                     /locus_tag="IWO31_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0302708.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IWO31_002319"
     CDS             4657..5649
                     /locus_tag="IWO31_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0302709.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IWO31_002320"
     CDS             5665..6549
                     /locus_tag="IWO31_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0302710.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IWO31_002321"
     CDS             6598..8205
                     /locus_tag="IWO31_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0302711.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IWO31_002322"
     CDS             8206..9690
                     /locus_tag="IWO31_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0302712.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IWO31_002323"
     CDS             9867..10859
                     /locus_tag="IWO31_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0302713.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IWO31_002324"
     CDS             10911..11780
                     /locus_tag="IWO31_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0302714.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13693)
                     /gene="guaA"
                     /locus_tag="IWO31_002325"
     CDS             complement(12200..13693)
                     /gene="guaA"
                     /locus_tag="IWO31_002325"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0302715.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4214 a         2322 c         2833 g         4365 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttg
//
LOCUS       DACECS010000077        13701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.077, whole genome shotgun sequence.
ACCESSION   DACECS010000077 DACECS010000000
VERSION     DACECS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.077"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWO31_002326"
     CDS             complement(<1..902)
                     /locus_tag="IWO31_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0302716.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWO31_002327"
     CDS             1185..2153
                     /locus_tag="IWO31_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0302717.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWO31_002328"
     CDS             2266..2544
                     /locus_tag="IWO31_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0302718.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWO31_002329"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWO31_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0302719.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWO31_002330"
     CDS             3010..3756
                     /locus_tag="IWO31_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0302720.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWO31_002331"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWO31_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0302721.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWO31_002332"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWO31_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0302722.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWO31_002333"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWO31_002333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0302723.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWO31_002334"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWO31_002334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0302724.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWO31_002335"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWO31_002335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0302725.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWO31_002336"
     CDS             8493..9035
                     /locus_tag="IWO31_002336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0302726.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWO31_002337"
     CDS             9058..10614
                     /locus_tag="IWO31_002337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0302727.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWO31_002338"
     CDS             10628..11530
                     /locus_tag="IWO31_002338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0302728.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWO31_002339"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWO31_002339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0302729.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWO31_002340"
     CDS             12973..13395
                     /locus_tag="IWO31_002340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0302730.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag a
//
LOCUS       DACECS010000078        13682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.078, whole genome shotgun sequence.
ACCESSION   DACECS010000078 DACECS010000000
VERSION     DACECS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.078"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            39..1403
                     /locus_tag="IWO31_002341"
     CDS             39..1403
                     /locus_tag="IWO31_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0302731.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISSTESSTTSSSTSEI
                     TSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNE
                     SVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYN
                     GNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKA
                     NSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHN
                     YSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGST
                     ALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTL
                     LFGD"
     gene            1752..2384
                     /locus_tag="IWO31_002342"
     CDS             1752..2384
                     /locus_tag="IWO31_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0302732.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2972..4411
                     /locus_tag="IWO31_002343"
     CDS             2972..4411
                     /locus_tag="IWO31_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0302733.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4706..4924
                     /locus_tag="IWO31_002344"
                     /pseudo
     CDS             4706..4924
                     /locus_tag="IWO31_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5173..5388
                     /locus_tag="IWO31_002345"
     CDS             5173..5388
                     /locus_tag="IWO31_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302734.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5605..5861)
                     /locus_tag="IWO31_002346"
                     /pseudo
     CDS             complement(5605..5861)
                     /locus_tag="IWO31_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6048..6959)
                     /locus_tag="IWO31_002347"
     CDS             complement(6048..6959)
                     /locus_tag="IWO31_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302735.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6978..7982)
                     /locus_tag="IWO31_002348"
     CDS             complement(6978..7982)
                     /locus_tag="IWO31_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0302736.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7979..10102)
                     /locus_tag="IWO31_002349"
     CDS             complement(7979..10102)
                     /locus_tag="IWO31_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0302737.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10107..12554)
                     /locus_tag="IWO31_002350"
     CDS             complement(10107..12554)
                     /locus_tag="IWO31_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0302738.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12541..12930)
                     /locus_tag="IWO31_002351"
     CDS             complement(12541..12930)
                     /locus_tag="IWO31_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302739.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12990..13493)
                     /locus_tag="IWO31_002352"
     CDS             complement(12990..13493)
                     /locus_tag="IWO31_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0302740.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13506..>13682)
                     /locus_tag="IWO31_002353"
     CDS             complement(13506..>13682)
                     /locus_tag="IWO31_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302741.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4203 a         2699 c         2501 g         4279 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt agtacgattt
      301 caagtaccga atcttcaaca acttcaagta gtacaagtga aataacttct tcgaaagtga
      361 caaaaccaaa ccatatagaa tcttcaaaaa aggaagcgcc aaaaacggaa ggacaaacaa
      421 aaactttttc gacaccacag caaatagaat caacagaatc tttccaatca caaccaatac
      481 tatctggtaa tgaagaaatt cattttgaca aaaatgaatc tgtagaaaat tttattatga
      541 aaattggtga gtctgcgaga aaaattggtc agaaatatga tttatatgct tctgttatga
      601 ttgcacaagc gatcttagaa tctgccagtg gtcaaagcca gttagctcag gcaccgaatt
      661 acaatttatt tgggataaaa ggaacttata atggaaactt tgtcattatg gtaacaaacg
      721 aagatttggg taatggaacg ctttatacga cccaatcaaa atttagagtg tatgaaaact
      781 atgaggaaag ctttgaagat tatgccaaat tattaactaa gggaattagt ggaaataaag
      841 atttttatgc aggagcatta aaagctaata gtaaaacata tcgagaagca actaaattcc
      901 ttacaggacg gtatgcaaca gatacacaat actatttgaa gctaaatgaa ttaattaaaa
      961 cctacgattt aaccaactat gataaagaag ttaaaggtcc acaaatgagt aagactggat
     1021 accaagtgcc tattcataat tattcaataa ccagtttgtt tggaaatcgt ggaaatgaat
     1081 ttcaccgagg acttgatatg gcagctacac agggtgagcc gattcatgca gttaaagctg
     1141 gtaaggttgt taaagcagag tttcactact cttggggaaa ttacgtggtg atacaacacg
     1201 aagatggatc aaccgcgtta tatgctcacc agcaacaata cctagtaaaa actggagata
     1261 atgttttgca aggacaaata attggctatg taggaagtac tgggaatagt actggcccac
     1321 accttcattt ggaaatttgc aaagatagta gtctttctca gagcatgtta gttgatccga
     1381 aaaccttatt attcggagac taaaaaaggt aatcaatctg acaaagtaac aagaaaatgg
     1441 ttggattctt agacctttga tcaggatatt gagactggaa ttgatatctc gattacgatg
     1501 ggtttgacaa ataaaacaag tccattatcg aatatcgagt gtattattgc cgtctttatg
     1561 ctcatatttt gagcaggttt cactagatga aaacaattta tctatcttga ggatttcgtg
     1621 tacgtatcgt tcagtcttgt attgtaaatt agtcataaaa tcagactaag aaaaattgga
     1681 aatatttttt tctgatttat taaaagttaa ttgaaattat atggtacttc tttcttggga
     1741 gggtaggtgc agtgaaggaa agatatggtc accttactgt tgttgaggag attactcata
     1801 atggatataa aaaatatcgt tgcctatgtg actgtgggaa tgaaacgatt gtatatcgat
     1861 caaacttact atcaggtaga accaaaagtt gtggatgtgg aaattataaa aataggcata
     1921 aatataaaaa tttgacaaat aaaacttttg gtaagttagt tgcaatcaag ccaactaaac
     1981 tgcgaagtga aggaactgtt gtttgggaat gtcaatgtct gtgtgggaga aaaaagttag
     2041 tatcggcaaa gcgacttcta aaaggtgatg taaaaagctg tggatgtttg aaatcaaaaa
     2101 agcatcacat tgaaaataaa aaattcggat tacttactgc aaagtatcct ttggacaata
     2161 actgttcatg gaaaacaaaa tgggtttgtc attgtgagtg tggcagtatg tgtgagataa
     2221 gttatagtaa cttatttttt ggacatactc aaagttgtgg atgtttaaaa aggcaagaaa
     2281 caaggacatg tgttgaaggg acagtaatag agtgtctaac gtcaaaatta ccgaaaaata
     2341 atacaagtgg tgttaaggga gtttatcgtt ctaaagagaa atagattgcg tatataactt
     2401 taaaaaaaaa gaggtactat ttaggatcgt atgattctat taagagtgca gctcaagcta
     2461 gaaaagatgc tgaaaataaa ttgtttcaac ctttgattga caaatatcga ggtaatcatt
     2521 aattattgtg gataataatt aacagtgata tataaacatc tataatatat tattcatact
     2581 attgatattt agtataatcc tatggaattg aatatcatag caatgtattt gaaaagcaaa
     2641 ttggaaaaca aataggtgca acaccataaa tatgaaaaaa ttgggaaaaa gtagaatgga
     2701 aatttgctta cgtctcacga gatagctcac actattaatc tgaagaacaa tgaagtcgac
     2761 ttttagaaat tgctagaaga aagtaaagcg aattatcact agttactata gagtaagttc
     2821 aaataaagca tctccaactt acatggttac atgaggatat caaatcgaaa ttactcaagc
     2881 ttcctatggc tagagtcgcg agaagttgtt gggcagtaga cataggttat attgttctta
     2941 gctacacgat cattagaaag gaaatcctat tatgtatagt tatcaagaag atgcaaaagt
     3001 ccaaaaaaat cgctggtgga ttttgattgc tatatctatg tttaccttca tgtccacatt
     3061 ggatggcagt attgtcaata ttgcactacc taccatctca gatgatatgg gcataccaat
     3121 gaatcaagcg gaatgggtag tgtccgttta tctgatgatt gtctgttctt gtttgttgct
     3181 gtttggtaaa attggcgata gccgtgggaa aatcaaagta tttcggattg gtacgctgat
     3241 ctttgtcttg ggatcgctgt tgtgtgggtt caatcattcg ctaggtattt tattgttcgc
     3301 acgggtggta caagccattg gtgctagtat gactatggct acaaatgccg gaatcatcac
     3361 ggaagtcttt cccatacatg aacgtggtcg ggcattggga gcaataggtg cgtttgtttc
     3421 attaggctcg attgctggac caggtcttgg cggcttgatt ttgtcccaat tttcttgggc
     3481 gtacattttt tggatcaatg taccggtggg aatcgtaacg atgttgattg ctgaaaaatt
     3541 cttaccaaaa gatatcacca tgagcggcca tacaatcgat ggagggggct ttgctgccat
     3601 tgcggctacg attatcagct tttttggagc aatctttttg gggcaagaaa gtggcttttt
     3661 ccatccagtc ccccttgtac tgttcgtaat ttcagtgatt gcctttgtca gctttttaaa
     3721 agttgaaaag aaagcagcct ctccattgat ttctttcggt atttttaaga acaagatttt
     3781 taccatgagc ttgattacag ccttgttgat tttttcttcc aacttctttg tcaatgtagt
     3841 gattcctttt tatctgcaaa atgctcgcgg gttgagtcct agctatgctg ggctgttgat
     3901 gatggtcttt cctttgatga tggtcgtcgg ggcgccttta agcggctacc tgaccgaccg
     3961 ggttggcccg gagattttgg tattagtagg cttgggctta ttgtcggtaa cccaggtcat
     4021 gtatatgttt ttgtcagtgg actctccttt gtggctatac attgtcgcta ctgccatcat
     4081 gggtgccggc aatgccttgt tccagtcccc caataatacg atggttatga gctcggtcac
     4141 tatcgaaaac ttgggactgg caggctcgat gaattccttt gcccgaaatt tggggatggt
     4201 cagcgggatt tcgctttcta cgacggtctt atatcaagcg atgtctgcta agatgggcca
     4261 gcaagtaact aactatatcg caaatcggcc ggatgtcttt ttatatggaa tgaagattac
     4321 ctttttaggc agctttgttt tgtgttttgt tgcctttttc ctgactatct ggcgtatcaa
     4381 aaaacaagga ggtattcaga aaaatgtctg aaaaaaccac tgttgaagaa tttttggagg
     4441 cctgcgtatg ggtgagtggg atttccatgg atgatggaag atgaagtgaa cttctaaaag
     4501 ccgaaaaact caagtattat cttcaaaata ctagtgctca atgagatgct tgatttgatc
     4561 acaagaagac tgcgaaagcc gctggcaacc gcaagctagg tatcccaccg atggactacg
     4621 ttttagaggc tactggttat gaatacggag ccaacgctct catctgcata agaatacatc
     4681 acccagatta tgtaaagatt ttcgatactt ctgtcaaaaa atcattaaga tagataaatg
     4741 atttccgtct agtcaaattg gttcagaatg tagacacaca gatggtaaga agtcactcac
     4801 aattcgagaa tggacttgtc ctatttgccg tatacatcat aatcgaaacg tcaacgatag
     4861 tatcaatatt ttgaccaagg gctatgaata caagcattgc cttagaaaac atcaagaacc
     4921 ttaggaacaa cggggatagc ttggttaata agagataccg ctgtttaggc ttgcaagtat
     4981 actcttttat caagaattcc gtggctttag tcatgagagg cttaatacta tgaaaatttt
     5041 ctttagcatt tttgatttag gttccttttc taattataat aagatgacca ctacagtaac
     5101 ctactgaaat atataaacag ccactgtagt accattttca aagtagtcta tgagaggaga
     5161 atcttcattc ttatgaacgc tggaattgcg gatatgaatt taataaaaaa aacccttaat
     5221 gattttactt ccaattcaat atcaaaaggt actggtatca atttaagcac aataaaaaag
     5281 ctgaaaagtg gtgaaagatc agtggaaaag ctgaatcttt tagatgctat aaaaataacg
     5341 gaatttgcta tgaaaaatgg aaaagctgag attgaaatat ggagataatt atcgtctagt
     5401 gaattacttg ccacttagct gaatttaacg atccttggtg cttgaagggg gagattcttg
     5461 ggaatagagc atacttgcaa gcctatgtct ttactaacaa cgatatctct tattaaccaa
     5521 gctatccccg tagttctaag gcttttactt ttatctaagc aataatcgta ttcaaaggaa
     5581 atttttgacc cttagaaagt ctcattttat cagacgtgag cttaggcatt gctgaatttt
     5641 gttaattcgg ttttaggttg tgctttgagc ataatatgaa agtattcttt atcacgattt
     5701 cattcatcta aggtaatgtg atgtgattct gaaattcttt caaaggcagt ttttgcataa
     5761 gctgacatta catcatcaat gacatgttta caatgtttca ctactaaaat aagggggtaa
     5821 ccaagaagaa aaactgaatg attatttgta tctaattcca tggtatatca accatttcct
     5881 atatagactg attatgacat ggattaaaat aaaaaggcac tatgtcttaa ttgtcggttt
     5941 tcgagttacc gaatggcatt catcacccac ctatagagga tgggcgactt ctgcctgaaa
     6001 taagttacac tacttataac ttctaataaa attagattga gtcattatta tttaacatca
     6061 acgattttcc agtttccatt tttttgtaat actaatttgt actgaaagta gctagtggtt
     6121 tttgatacat tatcaaggta ttttatcttt gtactcacta gaaaattttt tccgttttgt
     6181 agataaactg gattctctaa ctccgaaaac tttaagtcaa tattgattgg tcgcatcgca
     6241 ccattttcaa cataatactc caattctttt tcagaagctg atggataaag tttaaaaaat
     6301 gtttctaaaa aatcggtgat ttcttccgtt gttttggcat caatttctga atcgttttct
     6361 acttgttttg cttcatattt tgccttgtct ggctttccaa tcattgttgg caaactagtt
     6421 actaaatgat tcctgttttc atcttcaaag attgtcactc gatatgcaga agaaattgat
     6481 tgcgtctttt ttccctctgt aatatgttgc tccactaaaa atgaaacgac aaattcttcc
     6541 gagtgttttt caacatctag aatttttaca gattgaactt cggaactcgt gggaatatct
     6601 gctcgaacca tatcttggat taaagcgaga ccgttttctg taaggtactg ttcgagatta
     6661 ctcattctct tttcaattgc ttctttgtta tttttccatg aaaaatattc tttcgcaaaa
     6721 tcctctgtaa aactttcaac gccagaagta ttcactacct tctcttttat aaccactttt
     6781 tcatgagttg tatgttgatc aatggcggtg aaatttttat aaacgccaaa agcaagacta
     6841 caagacaata aaagccaaag ggcaagcacc atttttcgat gcttacccac ggatacaact
     6901 cgttcttttt tcttttttgg gctcttttcc ttttgattcc gttcaatttt tatcttcatt
     6961 ctttttcctc ttttctctta tttgtatcgg ccagctccga taagatgctg ccgccaatag
     7021 ttttccgtta gattcgtcca ccccactgga tctcctgcat gaaacatgcg attctcccct
     7081 gcgtaaatgc ccacatgggt cacataagta ccggcatcat aggttgaatg aaagaaaact
     7141 aaatcacctg gtttggcatc ttttagatca atatgagtca tggcatcata ttgatcctga
     7201 gccacccgag gaagtttcag gtcaactttg gcataacacc attgggtcaa gccactacaa
     7261 tcaaaccccg tttctggact tgagccacca aacacatagg ccgttccttc atacttaaat
     7321 gcctcatcaa aaatttcttg gactattttg tcatcaaact gagttgtagt taggtattgc
     7381 ttcactaata aacagtaaaa catattgcca taattatacc gccacccacc attttcctta
     7441 atcgcgattg ggttggggta atcgactttt tgaccacctg atttttcctt tgaaaaagat
     7501 tcagctaaag caaaactatg cttcttcccg tttttcccga catgatctag atacccgcca
     7561 ccatagttat aggcctgaat aatactatct tgatcacacc ctagttcttt ggaacgctta
     7621 accaaagacg caaaatagag acagccttgt ttgatggatg cctcggtact taatgaatta
     7681 ggtggtaatc cggctgactc agaactttgc ataacgtctt ccattgtgcc accagattcc
     7741 acttgaataa tcgccaaaag aattggaata tactctgtta tatccgattc tttggcatac
     7801 ttttccacca ttgcacggtg tttcaatact tcttctgaga cactagttcc gccaggagaa
     7861 gtgccagaac taccatttcc ctcactgtct tcaccaaaca acaaacccac acacatcaaa
     7921 atacccataa aagagatggt cagaaaagtt aatgaaataa cacccacttt cttcaagttc
     7981 attttttctc acctgacctt gattttggac gatggttcat tcttttttgt actttttgat
     8041 tggtctgctt tggcagagtg gacctctgtt tcacttgttg gaaagaattt ttcttacttg
     8101 ctgattggct tttccctttt gaaatgattt cttgactagt tagttttcgt ttgatttcgg
     8161 gattcagatt tgctctcaca atccgctttg gtgcttcttt tttcagcatc atatctttct
     8221 gaggttgagc tgctttatct attggaatat tcgcccttct ttttccctga cgatttgttg
     8281 cttcactcag tatcttgcgt ttttcgtcca ttttcgcatt tcttcgttgt cgttgttttt
     8341 ctcgcaaatc cactcgatcc gctttttctt ggaacagtcc gcgcttgaat tcttctggtg
     8401 cttttttcga cttttcgatt cctttatgga gattgtattt caaattcgtt ggcgtattcc
     8461 ttacctgatc ctttacttgt ttagtcttat ctttcaccaa agcttgggta tccaaaactt
     8521 tgccgatttt tctcccagcc aaattcattc gggattgttt cttattattt gaaactggat
     8581 tctccttagt taagtcagat agtatttcgt gatcgttcgg taatcgtgaa ttctttcgta
     8641 gagaagaacc ggatggctta aatttggatt ttgattgttc ctttgccaac ttatttccga
     8701 ctgctgcacc accaccaaaa gccaaagctc gtcccacatt tcgattgagt tgccgcaatt
     8761 ttcgattcat taacatttgc ggttttcgca tgacgcgtct tcccattgac tggctatcat
     8821 tactctggag gttgaacata ctcataatgt cgcccaattt gaaatagata ccggcaaagg
     8881 tcacaatttg cagaaaagca accatgaaaa atggataggt tgccgaaata ttgaaaaaca
     8941 tcgtggagat gctaaatgca gtcgtaatca ccaaagtaac acccgctcgc atcataatcg
     9001 tattgaataa acgaataatc gccttctttc ctaaactttc ataagtcggc aacatggaca
     9061 ataagaaact aattggcaga aacatcgcaa agataatgaa gagaatttgc gagaacaaca
     9121 taatccctgt tagaagaaaa acaaagaagg aaatgccaat attgaacaat accaaaaaga
     9181 ccacggtccc taaacggttc atggtcttgg taatactgag attcgcattg tcattgtctt
     9241 caatctctgc cttgaccgct tcttcccgat ctttgccttt gttttcatct ggactcacag
     9301 atagaatctt gttgacccgg tcttcaccaa tttcttctat gttggaatca tcaaattgca
     9361 gtagtaacca aggttgttgg acttgaatcg aaaacaaact atcccgaatc aaatccacac
     9421 tatcttttcc ttggctttct gaattgggta caacgatctc ggttccaagg gtaagggccg
     9481 cttcactaac atctgagcta aagtcattga tttttgtgat ataggtaggc gcataagcaa
     9541 tgaacgaacc cgatagtaga aaaatcatca caaaattaat cacggctcga actgctttcg
     9601 tggtttctcg ttttaataat ccggtatacg ccacatacac ccccagagct aaaatcatca
     9661 ataatagaaa cccaaaataa aatccggagg tctggagtcc attttcagta ataccagcca
     9721 atgtttggat attctttcca atactttcag ccgtatccga aatgaaatct aatttgtaag
     9781 cctcttgaac cacataaccc gtggcgttcg acaaatacaa actcaccgtc caaatgaaat
     9841 tagtaatggc ataaagacca tacatcacgc ttttccctaa gccatcaccc caattccaag
     9901 gcaaccaatc ccaagagcta tccacaaaga aatctagttg atagttgttc agtgaatatt
     9961 tggaatatag atttcctgct tcaactgtat catcaactag ccctgtcgcc tcggctaccg
    10021 taccaactaa actcaataga actatcagag tgagtatgct aataaaaacg attcccacaa
    10081 tccgaaaaag tttctttttc atcccctcac tccattctct cttgaacagg tggacgggta
    10141 tcaaaggcat ggaagagttc ttcaaagact ggatgtacct gaaccacgcc tacccgacca
    10201 tacaaatcct gcatcaagca ttggccattt tccaaatctc gtaggcgttt ttgattgctt
    10261 tcatcttctc gatccaagcc aaagaattcc aaggttttct tgatttcatg aatatcggta
    10321 ctgcgaaacg caaacttcaa cccgatatta ttcttcaagc gttcatccag taaatcatca
    10381 ctattttgcg tgacaaagta aaccccggca ttcatagccc gcccagcacg aacgagctta
    10441 ttcgataatg ttttcccttg cgctacttga agaaaactcc acgcctcatc taagtctaca
    10501 attttgaaga tttctcgatt actatggata aagtccaagg caaaagtgga aatgacgatt
    10561 agcatggaaa cactaagcaa ttccatggtg gtatattctt caaaagtggt ttctgcatct
    10621 ggtaaaacca aatccgctac ttgaatgata tttaactgcc tatctaaact aatcgactgg
    10681 gtaatcgaac catctgagaa aagcaaatgg gcaaaatcat aatcggtaaa ggactcgatg
    10741 tgatccgcga tattttccgc aactggtgag ccttcttttc gaagctcatt aattacttgt
    10801 aataatcccc gttgattgct ttgggtaacc gtccgaatgg ccttacgcaa cactggaaac
    10861 ttctccccat ctcgactaga aattcctgtt aaaaaggtca gaatatcaat tgctagactt
    10921 tcagaatcct tctttttctc cataatcaca taaggatcaa gtaatccttg attttttggc
    10981 ttactagtta agttgagaat attgatttca tccgcaattt ctggcaaggt ttctttccag
    11041 ccaccccgtt ccccttttgg atcgacaatc acagcttgtc caccaaaaag gacggcgtaa
    11101 taaactaaga gattgttact aaaggacttt ccgccaccga gtgaaccgag gaatgccgct
    11161 gctagcgcat tggtaactga atccttgacc ccttgtgagg ccaatgccgg tttcaggtaa
    11221 acattgcgcc ccgtatctac gttataacca aagtaaatgc cttccaactc ccctaacatc
    11281 tgagtggcgc caaaaccaag tccggcgaga aaatcagacg taacatactg gatataatca
    11341 ttcatgtatc gcttactaga aggtaagaat tcttcatgta gccccatcat atcgccaaaa
    11401 ggccggacta gttttacatt ggtatcatca taaaaatcta gcacttcatc acaccggcgc
    11461 tttagttcat ctactgattc ggcacttact cgaaccacat agctcagttt gtacatggat
    11521 tctttggact gatccaaggt agtttccaat tcatctacgg aatccaatgc atctaagaca
    11581 ttggaatttg tttcattgtc agattgatag gcatggttgt ccaagtcttt taattccttt
    11641 ttcttattgc gaacggtagc aagtgccttt ttgttcgtca caatctccac attcatacta
    11701 gtgtcaattg ggaatgtaaa ttgttgttgc tggtaataga aaagttctga ggagggaaac
    11761 tccatttccc ccacaattgt attaattgtt aagtacgcta catatgattc atgattctca
    11821 tgttccatgc ataagtaccg gggcttttct tcaatcaaac aacgactggg acgtaataag
    11881 tcgtaacgtt tcaccaaagt ttccgacttc agctttttct ttggtagctg aaattcatat
    11941 tcttcaaatg gtgtaccctt ttcgccatag atatgttcaa tcaaatacat cagatcactc
    12001 ggtgtgactc gtctgacttt aaatcttcga gccaatttac tttccattag tttttcaagc
    12061 ttggtatatc ggcgaatttc ttcattggat aaggaaacaa aatcgcccat caaatgatga
    12121 ttcactccat agacaaattc ttgaaagccg gagaagaaag atttcttgag attctttaga
    12181 ttgacctctt catctgtcgc aatcagtttg aagccaataa aaaagcgata atcaatctga
    12241 ctatcgccaa tcatggatac taatgcctct gtctgtaaat caattcgttg tttcgctact
    12301 tctgttaatc gcccggtaat ttcctttttt gatcgttctt gcgtggcacg aatactactt
    12361 tctgtggcaa tttgtaaggc atgaatcttg ccttcacgat tttgcgcaat caattgacga
    12421 aagttatcgt ggacttgata tttctgttca ggtgacaaaa aggagtaatt gtaaggtatt
    12481 aactcatagt aggcaaaaca ttctccctct tgattaaaga caagattatt ttcaatatac
    12541 ttaatcgggt acatagttca cactccttac cagagtaatg ggttcgtccc atcgtttctt
    12601 ctgttctttg actttctttc ctgcgaaagt gaccttaaat cgtaagtgat agctaataaa
    12661 agatcgtaaa aatcccaatg gtttcttacc gtcaaacgtt ttttgcgaca tgaaccaagt
    12721 aactgccacc ggtataccta agtattttaa aaaggccccc tctatcatag atagtggagg
    12781 cacatgggca aatacgatga caaacagcaa ggataaaaca aaccaagcca tctggttaaa
    12841 agtcacagga aaaggcagtt gaaagtcatt aattgcataa atgacctttt caactgccca
    12901 aatactggtg tagcttttta ttttcttcat caaatcattc ctttaaaaag aaggatagtt
    12961 tagaaatcaa ctacccttca ataattttgt taatatggat actcaaaaac acctcgactc
    13021 gttaccaaat aattgcctcc aatctccaag tctctaccac acgcatcaaa atcaatatac
    13081 gtcttcaagt gttctggtac ttctccaaaa tcaccagctt ctacaatcaa gaaatatgct
    13141 acatcactca tatcatcgca atctggataa tagataatat catccacatg ctctaccatc
    13201 tcctcaaaag aattgaagaa agcatgttga atctcagaag caacttcacc aactggcgtt
    13261 ccttcaagtt cctccgctaa gccacacaac cgattaattt cctcaattgg cgtatactca
    13321 tcaatctcaa agggaagttc ataatcatga attgcatatt cctcgtattc gtcatttaac
    13381 ccaattcgtt ctttcacttc atcaaaatcg acaggtgggg taaaccacgc ccccaccaat
    13441 tcaccttcgt tatatttccc gaggttcgca atataaacac gcatctgttc catcgtgctt
    13501 cccccttacg caccgacaat tcggttaaac aaactgagta agacatcctt caccccagaa
    13561 gcattaaaga ccaatccgac cgcaatcaaa gcaatcacca aaaagccaat cagtttggaa
    13621 aactcccgct taaatcctaa aaacaaacca atgaccacaa ttgccagtaa gactaaggat
    13681 tg
//
LOCUS       DACECS010000079        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.079, whole genome shotgun sequence.
ACCESSION   DACECS010000079 DACECS010000000
VERSION     DACECS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.079"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWO31_002354"
     CDS             complement(<1..914)
                     /locus_tag="IWO31_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0302742.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWO31_002355"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWO31_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0302743.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWO31_002356"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWO31_002356"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0302744.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWO31_002357"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWO31_002357"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0302745.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWO31_002358"
     CDS             4440..5321
                     /locus_tag="IWO31_002358"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0302746.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWO31_002359"
     CDS             5318..6034
                     /locus_tag="IWO31_002359"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0302747.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWO31_002360"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWO31_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0302748.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWO31_002361"
     CDS             6751..7752
                     /locus_tag="IWO31_002361"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0302749.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWO31_002362"
     CDS             7749..9110
                     /locus_tag="IWO31_002362"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0302750.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWO31_002363"
     CDS             9070..10221
                     /locus_tag="IWO31_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0302751.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWO31_002364"
     CDS             10196..11365
                     /locus_tag="IWO31_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0302752.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWO31_002365"
     CDS             11540..12919
                     /locus_tag="IWO31_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0302753.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWO31_002366"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWO31_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACECS010000080        13394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.080, whole genome shotgun sequence.
ACCESSION   DACECS010000080 DACECS010000000
VERSION     DACECS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.080"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..707)
                     /locus_tag="IWO31_002367"
     CDS             complement(15..707)
                     /locus_tag="IWO31_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0302754.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IWO31_002368"
                     /pseudo
     CDS             792..968
                     /locus_tag="IWO31_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IWO31_002369"
     CDS             complement(1144..1713)
                     /locus_tag="IWO31_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0302755.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IWO31_002370"
     CDS             complement(1787..2110)
                     /locus_tag="IWO31_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302756.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IWO31_002371"
     CDS             complement(2264..3076)
                     /locus_tag="IWO31_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302757.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IWO31_002372"
     CDS             3430..4278
                     /locus_tag="IWO31_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0302758.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IWO31_002373"
     CDS             4423..5364
                     /locus_tag="IWO31_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0302759.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IWO31_002374"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IWO31_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="IWO31_002375"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="IWO31_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0302760.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="IWO31_002376"
     CDS             complement(9828..12014)
                     /locus_tag="IWO31_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0302761.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="IWO31_002377"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="IWO31_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0302762.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="IWO31_002378"
     CDS             complement(12236..12676)
                     /locus_tag="IWO31_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0302763.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="IWO31_002379"
     CDS             complement(12750..13289)
                     /locus_tag="IWO31_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0302764.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3761 a         2807 c         2381 g         4445 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcg
//
LOCUS       DACECS010000081        13003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.081, whole genome shotgun sequence.
ACCESSION   DACECS010000081 DACECS010000000
VERSION     DACECS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.081"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IWO31_002380"
     CDS             173..1309
                     /locus_tag="IWO31_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0302765.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IWO31_002381"
     CDS             1446..1790
                     /locus_tag="IWO31_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0302766.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IWO31_002382"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IWO31_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0302767.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IWO31_002383"
     CDS             complement(3485..4084)
                     /locus_tag="IWO31_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0302768.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IWO31_002384"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IWO31_002384"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0302769.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IWO31_002385"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IWO31_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0302770.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IWO31_002386"
     CDS             7290..8408
                     /locus_tag="IWO31_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0302771.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IWO31_002387"
     CDS             complement(8468..9760)
                     /locus_tag="IWO31_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0302772.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IWO31_002388"
     CDS             9910..10470
                     /locus_tag="IWO31_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0302773.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IWO31_002389"
     CDS             10599..10976
                     /locus_tag="IWO31_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0302774.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IWO31_002390"
     CDS             10909..12981
                     /locus_tag="IWO31_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0302775.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4238 a         2327 c         2801 g         3637 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tga
//
LOCUS       DACECS010000082        12736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.082, whole genome shotgun sequence.
ACCESSION   DACECS010000082 DACECS010000000
VERSION     DACECS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.082"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IWO31_002391"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IWO31_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0302776.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="IWO31_002392"
     CDS             complement(324..893)
                     /locus_tag="IWO31_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0302777.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="IWO31_002393"
     CDS             1079..1981
                     /locus_tag="IWO31_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0302778.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IWO31_002394"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IWO31_002394"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0302779.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IWO31_002395"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IWO31_002395"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0302780.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IWO31_002396"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IWO31_002396"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0302781.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="IWO31_002397"
     CDS             8549..8734
                     /locus_tag="IWO31_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0302782.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="IWO31_002398"
     CDS             complement(8796..9992)
                     /locus_tag="IWO31_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0302783.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="IWO31_002399"
     CDS             complement(10162..11598)
                     /locus_tag="IWO31_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0302784.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="IWO31_002400"
     CDS             11891..12700
                     /locus_tag="IWO31_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0302785.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2674 c         2249 g         4009 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctagtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc acgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgaca
//
LOCUS       DACECS010000083        12677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.083, whole genome shotgun sequence.
ACCESSION   DACECS010000083 DACECS010000000
VERSION     DACECS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.083"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO31_002401"
     CDS             6..305
                     /locus_tag="IWO31_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0302786.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO31_002402"
     CDS             378..1739
                     /locus_tag="IWO31_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0302787.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO31_002403"
     CDS             1761..2021
                     /locus_tag="IWO31_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302788.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO31_002404"
     CDS             2035..2787
                     /locus_tag="IWO31_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0302789.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO31_002405"
     CDS             complement(2881..3486)
                     /locus_tag="IWO31_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0302790.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO31_002406"
     CDS             complement(3539..4513)
                     /locus_tag="IWO31_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0302791.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO31_002407"
     CDS             complement(4891..5670)
                     /locus_tag="IWO31_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0302792.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO31_002408"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO31_002408"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0302793.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO31_002409"
     CDS             7161..8558
                     /locus_tag="IWO31_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0302794.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO31_002410"
     CDS             8639..9079
                     /locus_tag="IWO31_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0302795.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO31_002411"
     CDS             9227..9553
                     /locus_tag="IWO31_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0302796.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO31_002412"
     CDS             9554..10303
                     /locus_tag="IWO31_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302797.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO31_002413"
     CDS             complement(10383..10838)
                     /locus_tag="IWO31_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0302798.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO31_002414"
     CDS             complement(11265..11540)
                     /locus_tag="IWO31_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302799.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO31_002415"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO31_002415"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0302800.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2178 c         2588 g         3856 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccct
//
LOCUS       DACECS010000084        11667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.084, whole genome shotgun sequence.
ACCESSION   DACECS010000084 DACECS010000000
VERSION     DACECS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.084"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWO31_002416"
     CDS             complement(37..216)
                     /locus_tag="IWO31_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302801.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWO31_002417"
     CDS             complement(597..1304)
                     /locus_tag="IWO31_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302802.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWO31_002418"
     CDS             complement(1297..1734)
                     /locus_tag="IWO31_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302803.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWO31_002419"
     CDS             complement(1749..2000)
                     /locus_tag="IWO31_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302804.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWO31_002420"
     CDS             complement(2000..2206)
                     /locus_tag="IWO31_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0302805.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWO31_002421"
     CDS             complement(2643..2903)
                     /locus_tag="IWO31_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302806.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWO31_002422"
     CDS             2978..3181
                     /locus_tag="IWO31_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0302807.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWO31_002423"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWO31_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0302808.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWO31_002424"
     CDS             complement(3810..4709)
                     /locus_tag="IWO31_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0302809.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWO31_002425"
     CDS             complement(4722..5315)
                     /locus_tag="IWO31_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0302810.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWO31_002426"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWO31_002426"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0302811.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IWO31_002427"
     CDS             complement(7771..9231)
                     /locus_tag="IWO31_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0302812.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IWO31_002428"
     CDS             complement(9292..11664)
                     /locus_tag="IWO31_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302813.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DACECS010000085        11050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.085, whole genome shotgun sequence.
ACCESSION   DACECS010000085 DACECS010000000
VERSION     DACECS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.085"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(78..659)
                     /locus_tag="IWO31_002429"
     CDS             complement(78..659)
                     /locus_tag="IWO31_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0302814.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(842..1468)
                     /locus_tag="IWO31_002430"
     CDS             complement(842..1468)
                     /locus_tag="IWO31_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0302815.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1490..2821)
                     /locus_tag="IWO31_002431"
     CDS             complement(1490..2821)
                     /locus_tag="IWO31_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0302816.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2976..3377)
                     /locus_tag="IWO31_002432"
     CDS             complement(2976..3377)
                     /locus_tag="IWO31_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0302817.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3463..3669)
                     /locus_tag="IWO31_002433"
     CDS             complement(3463..3669)
                     /locus_tag="IWO31_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302818.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3764..5266)
                     /locus_tag="IWO31_002434"
     CDS             complement(3764..5266)
                     /locus_tag="IWO31_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0302819.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5284..5739)
                     /gene="argR"
                     /locus_tag="IWO31_002435"
     CDS             complement(5284..5739)
                     /gene="argR"
                     /locus_tag="IWO31_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0302820.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5837..6454)
                     /locus_tag="IWO31_002436"
     CDS             complement(5837..6454)
                     /locus_tag="IWO31_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302821.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6675..8048
                     /locus_tag="IWO31_002437"
     CDS             6675..8048
                     /locus_tag="IWO31_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0302822.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8123..9235
                     /locus_tag="IWO31_002438"
     CDS             8123..9235
                     /locus_tag="IWO31_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0302823.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9307..10944)
                     /locus_tag="IWO31_002439"
     CDS             complement(9307..10944)
                     /locus_tag="IWO31_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0302824.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         2911 a         2348 c         1991 g         3800 t
ORIGIN      
        1 caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct
       61 gtcacaacct ctaattatta aagaaccttt tgcgaagtta acaatctttc tttgatcaat
      121 tgaaaattct gcctaagtag tgatagatcc tgtgtgactt tcaacaataa gatacttccc
      181 tggatttcag caatcaacgc tttggcttga cggtctgctt cttcatcgga cagtttttct
      241 tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc attgattgac taattctagt
      301 agcaacagac ggtattcttc atccactgta ctcaactcaa taatcaaatt acctacagga
      361 cagccatgat aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa catttcttct
      421 aatgcttctt ctggctgttt tccagcattt aagatgtctt cgaacaattc tgtctgccat
      481 acgtccatat gcgcatggat caccgctaga caaacttctt tttttgaaga aaagtaataa
      541 taaaattgac cttttcctgt atcagaagct gctagaatat ctcggattga cgtttcatga
      601 tagccttttt tataaataag atctttggtc gcttccaaga tcttttcctt tgttttcaat
      661 gatattctcc tttttcattt ctcatagtcc tatattatac agtaaattca tcattttttc
      721 ttcttaatcg tttaaaaaag actgtgccaa attatctgac acagccctca ctataaaact
      781 gttctcatat agctagatgt tcattgattc aagaactgcg aggacaaaag caattcctat
      841 cttatgatag ttgctctaat acttcctctg ttgtcatcac ttgatctgca ataaagccaa
      901 agttcgtcaa cgctgcatcc attgcttctt ggccaggagc accaattgca tctgtcgcta
      961 ccacaacatt gaatccactc tctaccaatt cacgcatatg ggaatcgcta cacaagtttc
     1021 cgttcattcc accaagaatt actgtgtcga ttccattttt tctcaattgg agcgccaaat
     1081 cattgctttc tggtccaaag attttatgag gagagctgat gatcgtattt tcatccaaat
     1141 aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc ttccactgat tcatattgac
     1201 tttttctagc aaacatctgc tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc
     1261 atttatggtc atgtgggtaa aaataatgag gagaaataaa gagtttgtat cctttttctt
     1321 ttgcttgttt taccatttca atcatatttt cgatcgtatt gtatttttct aagatatctt
     1381 ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc gacttgcggg tcagtgagaa
     1441 ctactgctgt atttttatta ttgaacatgg tggattctcc attcgttatt tattttaata
     1501 catcatttag agcttcactc attgtcggat gagaatagat ttgatctctc aataacgtat
     1561 aaggaagttt tccattcata gctagcgaaa tcaagttgat gacttcatga gaatcttccg
     1621 catagatact tgcccctaga atcaagtcag tttcagggtc gactaaaact ttgaatacac
     1681 ctttaggatc ttctaatact tgtgctttag ggacaccgct tgtcatatac ttgaataatt
     1741 tgtaattgac gccttgtgct ttggcttgtt tttctgttaa acctacattg gataaagtcg
     1801 gagtgataaa tacactgtaa ggcacagctt tccggttatt cgttgttcgt ttattctcgc
     1861 ccttcaactg atccataatg attcgataat catctagaga gatataagtg aactgcaatc
     1921 cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca
     1981 cagcgattgc tccgcgatct gttagtttaa catctgtatt ttctaatcca agtgatgctg
     2041 tacttggttt tcttccagtt gcagccaaaa cttttgacgc ttggattgtt acttcttggc
     2101 catcttctgt aaaggtgacg gcagctgttg attcttggtc tgctacttga tctacagata
     2161 cacctagatg gaattcgatt cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat
     2221 cttcatcttc acgtggaaga aattcagaat gggcatcaag aacgacaact ttagatccgt
     2281 attcattgaa cattgatgca aattccaagc caatatatcc agccccgatg atcactaatt
     2341 tttccggtaa ttgttcctga tccatcgctt gcgtagaatc aatcaagtag cgacttgtat
     2401 tcaatccttt gattggtaaa atgattggtt cagaaccagt attgatgaaa atccgttcgc
     2461 cttcaacaat cactttttca ccattattcg tcacttcgat ttgatggtta gaaagaaaac
     2521 gtgctgtacc gtccatcact gtcccagttg cctcatcgct gatcatatga taatttttgg
     2581 cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag tcgattcctc
     2641 tttctccatt gaaaatcaat gatttagaag gaatacagcc gatgttgata catgttccgc
     2701 catacatacg tgttgacttt tcaacgacta aaaccgattc accttttgaa gctaacgcct
     2761 tcgctaaagt cttaccgccc tttccaaaac cgatcacaat attttgatat ttttccatca
     2821 tttttccctc gcttcttttt tacctaccga ttggtacaaa aatatgataa ccgataattg
     2881 tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt
     2941 cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac ttctacatcg
     3001 atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc tgcaattttt
     3061 tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc gatctgatag
     3121 ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc tgctgtcatt
     3181 cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata acctgctgca
     3241 aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat tttcatatgc
     3301 atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat cattgctgtt
     3361 tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact tggctataat
     3421 tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga ataccagaat
     3481 gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat
     3541 atttgttcca aaataaatag caatcttttc tacataatct tttatatgat tgattttaag
     3601 atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa taaccatatt
     3661 cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt aaccatacca
     3721 ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct gctgttaatg
     3781 attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc aacttgttct
     3841 ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag cttaaacgga
     3901 tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct tcttcatttt
     3961 ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct tctaatatct
     4021 tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct tgtacttcta
     4081 cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt tttaattctg
     4141 gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa tagttttcct
     4201 tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca agacggttaa
     4261 gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact tggatgcctc
     4321 ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc acgattcttg
     4381 ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta tttcgattga
     4441 tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg aaaatgatcc
     4501 caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct tctttattat
     4561 caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg gaacctgttg
     4621 aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca ccatttccgg
     4681 catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca atcccctttt
     4741 taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc
     4801 cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata tcttcttttt
     4861 ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga agatggctag
     4921 cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac ataaacatag
     4981 caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc agactttgga
     5041 tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc
     5101 attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct agtccccagc
     5161 cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact ggtaatgcga
     5221 ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa aagtcctcct
     5281 catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg
     5341 gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag ggtaagccac
     5401 ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata cgataaccat
     5461 gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc
     5521 atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt tttcattttg
     5581 actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc
     5641 gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt gttctgtaat
     5701 gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct ctcaatgttg
     5761 attatagcaa aagataagta atatttcttc atattttggt attttttact tttatttttt
     5821 gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc ttcatttttt
     5881 caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta gaagctcttt
     5941 catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt tcttcggcat
     6001 agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg aatactagtt
     6061 gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt gctatcgctt
     6121 ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct tttttcatca
     6181 ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga tagacagtat
     6241 acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt gcatagctga
     6301 ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg ccgatccctc
     6361 tgatgctaaa gaatggtttt ttaggaacct ctagtatttg cgggcttttt ttcatactat
     6421 agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga aattccattt
     6481 agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga cactattttt
     6541 cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc
     6601 gagagagagg cggtttctat ttttataata gaaatcactc tcttttttta cgaaattttt
     6661 tagaggtgat gtaaatgaca aaacaaaaac agctgctttt agtacctgca gtaattgcaa
     6721 cagggatgat gtcttttgcg ggcgtattga tcgagacggc catgaatgta actttcccaa
     6781 ctcttatcag acagtttgat ttaacgactg cacaagtcca atgggtaaca acgatttatc
     6841 ttttgatgat ttcgatcatt gttccgctgt ctacttattt gaatcgcaat tttagtctca
     6901 aaaaactctt tttagcttca aatctgcttt tcttggcagg tataatgatc gatttcttct
     6961 ctcctacctt catggtccta ttgtttggtc gattgctcca aggtgcagcg acaggtatcg
     7021 ctcttccgtt gatgttccat attatcttga catttgcccc cctcaatcga cgaggcgcga
     7081 tgatgggtgt cggtactttg acaacggcca ttgctccagc aattggcccc acttatggag
     7141 gcattatgac ttcacatttt acatggaatc atatcttcct gtttcttgtg cccgttttga
     7201 ttctttcttt gattatcggc tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt
     7261 atttggattg gataagcgtt ttaggaattt gtcttttgtt tagtggaagt ctaacatttt
     7321 tgagtacgtt gggaacagtt tccagctgga ttgctttaat catcggcggt attggctggt
     7381 tcctttttta ctatcgaagt aaaaaagtgg agcatccact gatccgactg tctattcttt
     7441 caaataaaac attccgcttg tttttgattg gcttcttagt ttatcagttt ttattacttg
     7501 gtgtatcttt tgtcttgccg aatttccttc agatcgttca aggaagtagt ccttttgtcg
     7561 ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct ttttccggga
     7621 aaatgctgga tcaatatggt ccgaagaagc caatttttgc tgggctgatt ctttctttga
     7681 ttggttggct agcattattc ctactcatag gacacgcttc agtgggtgta ttgattgctt
     7741 gtcatgtgtt ttatatgatc ggtatcggat tatcttacag caatatgatg accaccggaa
     7801 tgaatgcact gaatgaagaa ctacaaggag acgggaacgc aatgttcaat accttgcagc
     7861 agttttctgg cgctgtggct acttcattag tagcggtcat tatcaattac gtccagcatc
     7921 atacttccca taactatgaa gtgtctacta cattaggatc aaaagcagca ttaggcgtct
     7981 tgttgctgtt gcttctcgta agctttgctc gattcgccta ttatctgttc tttgctaaaa
     8041 aagcgtagat tccatatacg gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt
     8101 gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt atcggtggcg ggatcattgg
     8161 tatgactcta gccagttact tagatacatc aaaattcaat gttgctttat ttgatgaagg
     8221 gacaggacag gcaactaaag ctagcgctgg gattatctcc ccttggttgt cgaaacgcag
     8281 aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct tttttcccta aacttataaa
     8341 agatttccaa cttgatcaaa cgatttacgc acaatcaggg acacttttgc ttcgacctga
     8401 aaaagattta gctgaattgg ctgttcttgc cgagaaacga aaagaagaag cgccagaaat
     8461 tggaaacatc gaatggcttt cacctgaaca gacatctcaa gcacttccgt tattgaaacc
     8521 agcagtttca ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat atttagaaca
     8581 tcttggtcag cgtttagcac aaaaacaaat ggagcagatc caggcaaaag tcactttgca
     8641 taagaagagc gattcttggc tcatcaaggg accgtttgga gaatacacag ctgatcgtgt
     8701 aattttgaca cctggcccgg cacttaagcc tttattacaa gaattaggct atgctgcaga
     8761 tatacgtccg caaaaagggc agctcttagc atttcagaca tcttataaga tgagtcaaga
     8821 ctggcctgtt gctatgcttg acggagaagc tgatttcatt cctttcatgg atggaaagat
     8881 ccttttaggt gctacccatg aaaacgatca aggctgggat ttggctccta ctagcgaagc
     8941 ttataaggga ttgactaaga gtacagaaca gttcttgact gatccagatc agataaaaaa
     9001 ctggccatac gagttccgtg taggtacccg tgcttatacg tcagattttt ccccattttt
     9061 cggcgtactg ccagatgatc caagcttact ggctgctagc ggtctaggct cttctggctt
     9121 gaccacaggt ccttatatcg gctatctatt agccaacttc ttaaatactg gtcattggtc
     9181 tacggaagat tatcaaaagg acatctccac ctatattacg aaaaatccat catagaaaaa
     9241 cagctttttc aagtaaggat cccgagcgat attgcgcatg gattacttct tgaaaaagct
     9301 gttattttag ttcaatttct gaatcaaatt ctccaaactt tcttttgctt catctggcaa
     9361 gagatctttt cctatctgag cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac
     9421 cagattttta taggacgtat cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg
     9481 gatgtcttca aatggaccaa acgacttgaa ttctgccgtt tgttgcacga ttctttcaac
     9541 gattgacaat gtcgtttcag gtttgatttt tttatcatcg aaatatccag tattcaggac
     9601 atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag
     9661 aggatagaca cggaaaggat tagcaaatgg ttcaatgact agctcatcta gctgtctttt
     9721 tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc
     9781 taaaactggg tcattgatct tgataattgg cggaagcgaa tcatctttca tgatccaaaa
     9841 aacagcatcg atcttttcgc taagatgatc gactcggtcg ggtgtcgcat aacgtgattt
     9901 gactgtccgc ccatttccgt ttcgtatatc tcctgttact aggacttttt ctcctttact
     9961 tgtacatgtc acgcccacat tctgacaagt aaggaaagaa gaaatggccg gatcatcaga
    10021 cggatagtct tgtgttttat caaagtaaga aggttccaag gcagtagcaa aaccattttt
    10081 tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt
    10141 tgctaaggta atcgttgatt ttccagaacc ggacaaccca aatacagcca tcgtataggt
    10201 ctggtcatta ctagcatatt gtttcatccc accgtggcag gcaataaagc cattgcgatt
    10261 tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc
    10321 tagcacagcc gctgtattat ggagagcatc aaacaaaatg agtccatttg gaaatgtttc
    10381 atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg aaacagactt
    10441 ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa
    10501 attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt cttcagatag
    10561 gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca cagcttctct
    10621 taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc gccgagcact
    10681 tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat cagctggtaa
    10741 atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg gactcccttt
    10801 tgctagttga taagctgtct tcctgtcaaa aacttcatga acattattac cgtaaaatgt
    10861 cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga tttcgtttaa
    10921 ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc
    10981 tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag caaacgagtt
    11041 atttttcaca
//
LOCUS       DACECS010000086        10922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.086, whole genome shotgun sequence.
ACCESSION   DACECS010000086 DACECS010000000
VERSION     DACECS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.086"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWO31_002440"
     CDS             complement(<1..288)
                     /locus_tag="IWO31_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302825.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWO31_002441"
     CDS             430..1548
                     /locus_tag="IWO31_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0302826.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWO31_002442"
     CDS             1676..3052
                     /locus_tag="IWO31_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0302827.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWO31_002443"
     CDS             3453..5033
                     /locus_tag="IWO31_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0302828.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWO31_002444"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWO31_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWO31_002445"
     CDS             complement(5659..5979)
                     /locus_tag="IWO31_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0302829.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWO31_002446"
     CDS             complement(6127..8361)
                     /locus_tag="IWO31_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0302830.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWO31_002447"
     CDS             8840..9106
                     /locus_tag="IWO31_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0302831.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWO31_002448"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWO31_002448"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0302832.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3474 a         1911 c         2215 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 ta
//
LOCUS       DACECS010000087        10510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.087, whole genome shotgun sequence.
ACCESSION   DACECS010000087 DACECS010000000
VERSION     DACECS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.087"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IWO31_002449"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IWO31_002449"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0302833.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IWO31_002450"
     CDS             1085..2005
                     /locus_tag="IWO31_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0302834.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IWO31_002451"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IWO31_002451"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0302835.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IWO31_002452"
     CDS             3311..4789
                     /locus_tag="IWO31_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0302836.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IWO31_002453"
     CDS             5066..7126
                     /locus_tag="IWO31_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302837.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IWO31_002454"
     CDS             7209..7778
                     /locus_tag="IWO31_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302838.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IWO31_002455"
     CDS             complement(7953..8759)
                     /locus_tag="IWO31_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302839.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IWO31_002456"
     CDS             8897..10411
                     /locus_tag="IWO31_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0302840.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3725 a         1752 c         2073 g         2960 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acgagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACECS010000088        10154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.089, whole genome shotgun sequence.
ACCESSION   DACECS010000088 DACECS010000000
VERSION     DACECS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.089"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWO31_002457"
     CDS             140..460
                     /locus_tag="IWO31_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302841.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWO31_002458"
     CDS             912..1112
                     /locus_tag="IWO31_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0302842.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWO31_002459"
     CDS             1259..4048
                     /locus_tag="IWO31_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0302843.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRQMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWO31_002460"
     CDS             4095..4622
                     /locus_tag="IWO31_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0302844.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWO31_002461"
     CDS             4643..5833
                     /locus_tag="IWO31_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0302845.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWO31_002462"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWO31_002462"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0302846.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8347..9021
                     /locus_tag="IWO31_002463"
     CDS             8347..9021
                     /locus_tag="IWO31_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0302847.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9018..9359
                     /locus_tag="IWO31_002464"
     CDS             9018..9359
                     /locus_tag="IWO31_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302848.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9389..9748
                     /locus_tag="IWO31_002465"
     CDS             9389..9748
                     /locus_tag="IWO31_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0302849.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9952..10131
                     /locus_tag="IWO31_002466"
                     /pseudo
     CDS             9952..10131
                     /locus_tag="IWO31_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         3393 a         1810 c         2174 g         2777 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgccag atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 ccttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagtgcctt
     7441 gacctgagta ttagagaaac aaaacggaaa aatagcttct catattttac cgttttttta
     7501 gctaatatcg agggtcagct gattgaacac cgtttatatc aagaggttgt gcgcctgccg
     7561 ttcagctccg agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt
     7621 gtgacttgtg accgaggagt tggccggaga gcaacgttta tttaaaaaaa agaactgcga
     7681 actctcgcag ttcttttttt aaaatacggg ataaaggaag tttccagcag acaagagaag
     7741 aaagcaaaag aaattgctat ttgattcaca aggttctata acgaaatcgc aatcaatcaa
     7801 acatatacaa agaaggttat tcaaatggaa atctgatttg atgatgcttc tgctgttgaa
     7861 ataaagcagc atctagtacc tgaaaacaaa ctaatagtaa cattcgaaga tggagtaggt
     7921 ccgtattttc agcatgcaat gatccatatg taagtttaat tcatccttga aatcatcgga
     7981 ccagataaag ttatgaccaa tctatcccaa gcaatctaga agagatatta gtaaaagaac
     8041 atttactgga agtcttagat gagcctataa atatatgttc tgatggcgtc atcagtggtc
     8101 tgattgacgg aagcttaggt gtgatcgatt tctctggacc aaacaacgaa ggaatcaagg
     8161 ggaatccggc acgttaagaa aaaaagtata aaaaactaga cacttgatga gcgttcattc
     8221 aattgattga agggactagg ctggtgtcat tttttataca tatattttct ctttcgtctt
     8281 gatagggctt atgggataat ataaggaaga aaatcatcgt tttattcaaa aaggagtttt
     8341 tgacaaatga aaaaacgtat attatttatc ggtgacagca ttacagacgc aggaagagac
     8401 cgagcagaag caactgatct aggtaaaggt tatccacttt tgattgccgg aaaactaatc
     8461 tcccaatatc caacgatgaa atttgaatgc atgaaccgtg ggataggagg agacaaaatt
     8521 gcagatctgc aagtacgctg gcaagaagat tgtttagctt tacaacctga ctatgtatcg
     8581 attttgatcg gaatcaatga tacctggcat aatactagcg atcaggctat attcggaact
     8641 gagaaatctg cgcagcaatt tgaatccgtt tatcgaaaat tactaaatga acttcaaaca
     8701 gcggaaatct gcaacatcat cttgatggag ccgtttgtac ttcctataac cgacgaccgt
     8761 catggctggc gtaaagactt agaccctaaa atccaaattg ttcgtaaact tgctaaggaa
     8821 tatggcgcat tgctggttcc tttagatggc atattcaatg ctttagggat tgcttatggt
     8881 ttttcgaaat atacaagaga agatggggtc cacccaacga ttggcggaca tgagatcatt
     8941 gcacaaagct ggatagatgc aattgccgca gtcgattttt ttagtggaga taaaaatgag
     9001 cgaaagaaaa gtgagaaatg atggaaatac ggtgggcagt aaaagaagat ttacctcaaa
     9061 tcaatcagct gattactgat catccagaat cattcgtaaa agcaaaaacg gcagctgacc
     9121 taaatgataa gaataaacta ttgctcgttg ctgtaacaaa agaaaaacgg attatcggat
     9181 ttgcgcaagc ggacgtgcaa cagtcagaag aacgggtgga tcagcgacca tcaggaaaac
     9241 taatccaaca cacattagta acaaaagaaa atacggaaga aatccaaaaa aaatttgcag
     9301 aatacttgat gctggaagct aaaaaaagaa gaataaaatc aatttttgcc cagttttaat
     9361 caaaaacagt aatagaagga gaaatacaat gagttgttta ggaaatgtca tctggtttat
     9421 attcggcgga ctaatcggtg gtcttagctg gttagcagcg gggatacttt ggtgtatcac
     9481 gatcataggt atcccaatcg gtatgcaatg ttttaagatc gcctcccttt cttttgcgcc
     9541 ttttggcaaa gaagtaattt atcaccagtc tggcatgtca ttgatcgtta atatcatctg
     9601 gctgatcgtt tcaggcctac caattgctct cgcacatgtc gtcagcggca tccttctatg
     9661 tatcacgatc atagggatac cttttgccaa gcaatctttc aaacttgctc gattggcgtt
     9721 gatgccattc ggtgcagaaa ttgtataaaa agagaataat aaaggttaga aaacgcatag
     9781 agtcaggtgg gatacatgta tttcacctga ctctatgtgt tttcctgtat aagttctccc
     9841 atgtgacttt tacgtctttt atttatatgg gattactgtt tatggaaagt tgaaataatt
     9901 ttcattaaag aactctttct atatttttga aagcgtagtg gagcttcgaa tatctgggaa
     9961 ggacattttg cagggattga aaaaagatat tgtcctatcg aaaaattaat gaatttagac
    10021 aaattgccag caacaggctt caaacttagt tgctatcctg tcaagatcaa gaaggcaagt
    10081 gcgggttgga taagagcagg ggcaatgatc gaagagaaaa agaaagaata agccaaaaca
    10141 tctgtatgga agaa
//
LOCUS       DACECS010000089        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.090, whole genome shotgun sequence.
ACCESSION   DACECS010000089 DACECS010000000
VERSION     DACECS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.090"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWO31_002467"
     CDS             complement(247..759)
                     /locus_tag="IWO31_002467"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0302850.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWO31_002468"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWO31_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0302851.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWO31_002469"
     CDS             complement(3212..3646)
                     /locus_tag="IWO31_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0302852.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWO31_002470"
     CDS             complement(3656..4444)
                     /locus_tag="IWO31_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0302853.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWO31_002471"
     CDS             complement(4441..5382)
                     /locus_tag="IWO31_002471"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0302854.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWO31_002472"
     CDS             complement(5559..6146)
                     /locus_tag="IWO31_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0302855.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWO31_002473"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWO31_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0302856.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWO31_002474"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWO31_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0302857.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWO31_002475"
     CDS             complement(9063..9998)
                     /locus_tag="IWO31_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0302858.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2777 a         2298 c         1746 g         3265 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgtaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACECS010000090        10075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.091, whole genome shotgun sequence.
ACCESSION   DACECS010000090 DACECS010000000
VERSION     DACECS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.091"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1481
                     /locus_tag="IWO31_002476"
     CDS             <1..1481
                     /locus_tag="IWO31_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0302859.1"
                     /translation="SDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRVE
                     SLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFVF
                     LLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAIIKLFNTILTRAGITLIIT
                     TAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLSDLMSMFSLQSNDSQSVGSRVM
                     RKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSSGRTQADHTRPDGQE
                     KSTLGKRIGQTIGAVADTKDKIVDSAGNLKEQVKDIPTNARYAVYQGKSKAKENVRDL
                     TSSISQTRADRASGRKEQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQEQSH
                     DGQTAKQSSIQTSRMESQQAKQERPAVKSDSLSLKAERQNRTTQERTVQKPATSTTPA
                     DRASQRPVTKERPSTVQRVQSQNLRNRPPIKSATIKKGSKKP"
     gene            1478..2479
                     /locus_tag="IWO31_002477"
     CDS             1478..2479
                     /locus_tag="IWO31_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0302860.1"
                     /translation="MKLKALVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            2476..3408
                     /locus_tag="IWO31_002478"
     CDS             2476..3408
                     /locus_tag="IWO31_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302861.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            3653..3769
                     /locus_tag="IWO31_002479"
     CDS             3653..3769
                     /locus_tag="IWO31_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0302862.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3785..5704
                     /gene="tet(M)"
                     /locus_tag="IWO31_002480"
     CDS             3785..5704
                     /gene="tet(M)"
                     /locus_tag="IWO31_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0302863.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVE
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            5814..5864
                     /locus_tag="IWO31_002481"
                     /pseudo
     CDS             5814..5864
                     /locus_tag="IWO31_002481"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            5898..7274
                     /gene="tet(L)"
                     /locus_tag="IWO31_002482"
     CDS             5898..7274
                     /gene="tet(L)"
                     /locus_tag="IWO31_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0302864.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKSPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGALIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPTITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIMVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            7838..9100
                     /locus_tag="IWO31_002483"
     CDS             7838..9100
                     /locus_tag="IWO31_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0302865.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            9345..>10075
                     /locus_tag="IWO31_002484"
     CDS             9345..>10075
                     /locus_tag="IWO31_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0302866.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPV"
BASE COUNT         3416 a         1640 c         2073 g         2946 t
ORIGIN      
        1 attccgatag tcaaggcaag gacagcgtgg acttaatcag agatagcctg ttttccatac
       61 aggttcagca accgtggcta ctgcttcaat acaacagttc agacattgaa agtattggtg
      121 ctgaccgtgt ggaaagtcta ctttcaacca gtccagacag taataatgga gaagacagag
      181 aaaaaattgt agcagaagaa atcgaagaca gaaacaatgt gaacatgacg attacaaaaa
      241 ctattaaccg tttgggtaca gtcttctttc tctttgtatt caatatcggg atttctatat
      301 tcgtattcct actaacagga atcatgattt tctcgcaggt actttttatc atctatgcca
      361 tgtttctgcc tgtgagcttt attttaagca tgattccgtc ctttgatggt atgtcaaaac
      421 gagccattat caaactcttt aataccattt tgacacgagc tggaatcacg ctgattatta
      481 cgacagcctt tagtatttca accatgctct ataccttatc ggctggttat ccgttctttt
      541 tgattgcctt tttacagatt gtgacctttg caggaatcta cttcaaactg agcgatttaa
      601 tgagtatgtt ttcgcttcaa agcaacgatt ctcaaagtgt gggaagtcgt gtgatgagaa
      661 aacctcgtat gcttatgcac gctcatatgc accgcctaca gcgtaaactt ggacgctcca
      721 tgacagcttt aggtgctggc tctgccattg ccagtgctac aggaaaacaa ggacagtcgg
      781 gtagttctgg taggacacaa gcagatcaca cccgtccaga cggacaggaa aaatcaacac
      841 ttggaaaacg tatcggacaa acaatcggag cagttgctga taccaaagat aaaatcgttg
      901 attctgctgg taatctcaaa gaacaggtta aggatattcc gaccaatgca agatatgcag
      961 tgtatcaagg aaaatccaaa gcaaaagaaa atgttcgtga cctaacttcc agtatttctc
     1021 aaaccagagc agacagagcc agcggacgca aggaacagca ggaacaaaga cgcaaaacca
     1081 ttgcagagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca gcttcttccg
     1141 ttcatgaaag accagctact aagcaagaac aatctcatga tggacagacc gcaaaacaat
     1201 cttctattca gacttcacgc atggaatctc aacaagccaa acaggagcgt ccagcagtta
     1261 agtccgattc tttaagttta aaagcggaac gacaaaaccg tactacacaa gaaagaacag
     1321 ttcaaaagcc agcaacttca actacaccag cagacagagc ttcacaacgt ccagtcacaa
     1381 aagagcgtcc gtctactgtt caaagagttc aaagccaaaa ccttagaaat agaccaccaa
     1441 taaaatccgc cactatcaag aaaggcagta agaaaccatg aaactgaaag ctttagtgat
     1501 tggtggttct gggctattct tgatggtctt ctcactgctt ctgtttgttg ccattttatt
     1561 ttcagatgaa caggacagcg gaatttccaa tattcattat ggaggtgtga atgtttccgc
     1621 agaagtgctg gctcataagc ctatggtaga aaaatatgcc aaagaatatg gcgttgaaga
     1681 atatgtcaac atacttcttg cgattataca ggtggaatcg ggcggtactg cggaagatgt
     1741 tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat
     1801 taagcaaggt gtgaagtatt tcagtgaatt attagccagt agcgaaaggc tcagtgtaga
     1861 tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttagggt atgtggctaa
     1921 tcgtggaaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg
     1981 cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa
     2041 ctatggcaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa cagagtttga
     2101 tgatgatacg gtacaagcca tcatggacga agcactgaaa tatgagggct ggcgatacgt
     2161 ttacggtgga gcttccccga ctacttcttt tgattgtagc ggactgacac aatggacgta
     2221 tggaaaagct ggaattaact taccacgaac cgcacaacag caatatgatg tgacccagca
     2281 tatcccacta tcggaagcac aagctggcga tttggttttc tttcattcta cctataacgc
     2341 tggctcttat attactcatg ttgggatata ccttggcaat aaccgtatgt ttcatgcagg
     2401 cgacccaatc ggttatgccg acttaacaag cccctactgg caacagcatt tagtgggagc
     2461 aggacgaatc aaacaatgag aaaggaagat ttaatgatga aatttagaaa aaatcagaat
     2521 aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa
     2581 aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag
     2641 cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac
     2701 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
     2761 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
     2821 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
     2881 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
     2941 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
     3001 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
     3061 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
     3121 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
     3181 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
     3241 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
     3301 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
     3361 gatttggtac ttgaaaagaa cgggagtaat tggaagattg taaaataaca aatattggta
     3421 catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg agattccttt
     3481 acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt aataaatatg
     3541 cggcaaggta ttcttaaata aactgtcaat ttgatagcag gaacaaataa ttagatgtcc
     3601 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
     3661 aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag
     3721 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
     3781 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
     3841 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
     3901 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
     3961 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
     4021 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
     4081 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
     4141 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
     4201 ggtttatcag gatattaaag agaaactttc tatggaaatt ataatcaaac agaaagtaga
     4261 gctgcaccct aatatgtgtg tgatgagctg tacggaacct gagcaatggg atgtggtaat
     4321 agaaggaaat gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattaga
     4381 actcgaacaa gaggaaatca gaagatttca gaattgctcc ttgtaccctg tttatcatgg
     4441 aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga ataaatttta
     4501 ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa ttgaatattc
     4561 ggaagaaaga caacgtcttg catatgtacg cctttatagt ggagtactac atttgcggga
     4621 ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata cttcaataaa
     4681 tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt tgcaaaatga
     4741 gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga gagagagaat
     4801 tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc aacaaaggga
     4861 aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac aatattatgt
     4921 ggattctacg acacatgaaa tcatactttc tttcttaggg aaagtacaaa tggaagtgac
     4981 ttgtgctcta ttgcaagaaa agtatcatgt ggaggtaaaa ataaaaaagc ctacagtcat
     5041 ttatatggaa agaccgttaa aaaaagcaga gtataccatt cacatcgaag tgccaccgaa
     5101 tcccttctgg gcttccattg gtctttctgt agaaccgctt ccattaggga gcggagtaca
     5161 gtatgagagc tcggtttctc ttggatactt aaatcaatcg tttcaaaatg cagttatgga
     5221 agggatacga tatggctgtg aacaaggatt gtatggttgg aatgtgacgg actgtaaaat
     5281 ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct
     5341 tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata
     5401 tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc
     5461 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
     5521 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg
     5581 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
     5641 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
     5701 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
     5761 ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtga aatgaatgta
     5821 acagggttca attaaaagag ggaagcgtat cattaaccct ataaactacg tctgccctca
     5881 ttattggagg gtgaaatgtg aatacatcct attcacaatc gaatttacga cacaaccaaa
     5941 ttttaatttg gctttgcatt ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg
     6001 tctcattacc tgatattgca aatgatttta ataaatcacc tgcgagtaca aactgggtga
     6061 acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag ctatctgatc
     6121 aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc ggggcgttaa
     6181 ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt attcaagggg
     6241 ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat attccaaagg
     6301 aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga gaaggagtcg
     6361 gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt ctactcattc
     6421 ctacgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa gaagtaagga
     6481 taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt gtatttttta
     6541 tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg tcattcctga
     6601 tatttgtaaa acatatcagg aaagtaacag atccttttgt tgatcccgga ttagggaaaa
     6661 atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca gtagcagggt
     6721 ttgtctctat ggttccttat atgatgaaag atgtttacca gctaagtact gccgaaatcg
     6781 gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac attggtggga
     6841 tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca tttctttctg
     6901 ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg acaattataa
     6961 tggtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca attgtttcaa
     7021 gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt accagctttt
     7081 tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc ttacttgatc
     7141 aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat ttgttattac
     7201 ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat aaacattctc
     7261 aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa ttgatccttt
     7321 ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc tgaataaaag
     7381 atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt tacactagaa
     7441 aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa gctagacaaa
     7501 acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt gatcttctca
     7561 aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa cccccctttg
     7621 ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct
     7681 taaaggtttt atggttttgg tcggcactgc cgacagcctc gcagagcaca cactttatga
     7741 atataaagta tagtgtgtta tactttactt ggaagtggtt gccggaaaga gcgaaaatgc
     7801 ctcacatttg tgccacctaa aaaggagcga tttacatatg agttatgcag tttgtagaat
     7861 gcaaaaagtg aaatcagctg gactaaaagg catgcaattt cataatcaaa gagagcgaaa
     7921 aagtagaacg aatgatgata ttgaccatga gcgaacacgt gaaaattatg atttgaaaaa
     7981 tgataaaaat attgattaca acgaacgtgt caaagaaatt attgaatcac aaaaaacagg
     8041 tacaagaaaa acgaggaaag atgctgttct tgtaaatgag ttgctagtaa catctgaccg
     8101 agattttttt gagcaactgg atccaggaga acaaaaacga ttttttgagg aaagttataa
     8161 attattttcc gaacgatatg gcaagcaaaa tattgcttat gcaacagttc ataatgatga
     8221 gcaaacccct cacatgcatt taggtgttgt gcctatgcgt gatggaaaac tgcaaggaaa
     8281 aaatgtgttt aatcgtcaag aactgttatg gctacaagat aaattccccg agcatatgaa
     8341 aaaacagggt tttgagttga agcgtggtga acgtggctct gaccgtaaac atattgagac
     8401 agctaaattt aaaaaacaaa ctttggaaaa agagattgat tttctagaaa aaaatttagc
     8461 agttaaaaaa gatgaatgga ctgcttatag cgataaagtt aaatcagatt tagaagtacc
     8521 agcgaaacga cacatgaaaa gtgttgaagt gccaacgggt gaaaagtcca tgtttggttt
     8581 gggaaaagaa ataatgaaaa cagaaaagaa accaaccaaa aatgttgtta tatcggagcg
     8641 tgattataaa aacttagtga ctgctgcgag agataacgat aggttaaaac agcatgttag
     8701 aaatctcatg agtactgata tggcgagaga atataaaaaa ttaagtaaag aacatgggca
     8761 agttaaagaa aaatatagtg gtcttgtaga gcgatttaat gaaaatgtaa atgattataa
     8821 tgagttgctt gaagaaaaca agtctttaaa gtctaaaata agcgatttaa agcgtgatgt
     8881 gagtttaatc tatgaaagca ctaaggaatt ccttaaggaa cgtacagacg gcttaaaagc
     8941 ctttaaaaac gtttttaagg ggtttgtaga caaggtaaag gataaaacag cacaattcca
     9001 agaaaaacac gatttagaac ctaaaaagaa cgaatttgaa ctaactcata accgagaggt
     9061 aaaaaaagaa cgaagtcgag atcagggaat gagtttataa aataaaaaaa gcacctgaaa
     9121 aggtgtcttt ttttgatggt tttgaacttg ttctttctta tcttgataca tatagaaata
     9181 acgtcatttt tattttagtt gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta
     9241 aagtattgaa aacccttaaa attggttgca cagaaaaacc ccatctgtta aagttataag
     9301 tgaccaaaca aataactaaa tagatggggg tttcttttaa tattatgtgt cctaatagta
     9361 gcatttattc agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga
     9421 gaccatggag agaaaagaaa atcgctaatg ttgattactt tgaacttctg catattcttg
     9481 aatttaaaaa ggctgaaaga gtaaaagatt gtgctgaaat attagagtat aaacaaaatc
     9541 gtgaaacagg cgaaagaaag ttgtatcgag tgtggttttg taaatccagg ctttgtccaa
     9601 tgtgcaactg gaggagagca atgaaacatg gcattcagtc acaaaaggtt gttgctgaag
     9661 ttattaaaca aaagccaaca gttcgttggt tgtttctcac attaacagtt aaaaatgttt
     9721 atgatggcga agaattaaat aagagtttgt cagatatggc tcaaggattt cgccgaatga
     9781 tgcaatataa aaaaattaat aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa
     9841 taaataataa agataattct tataatcagc acatgcatgt attggtatgt gtggaaccaa
     9901 cttattttaa gaatacagaa aactacgtga atcaaaaaca atggattcaa ttttggaaaa
     9961 aggcaatgaa attagactat gatccaaatg taaaagttca aatgattcga ccgaaaaata
    10021 aatataaatc ggatatacaa tcggcaattg acgaaactgc aaaatatcct gtaaa
//
LOCUS       DACECS010000091        10018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.092, whole genome shotgun sequence.
ACCESSION   DACECS010000091 DACECS010000000
VERSION     DACECS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.092"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWO31_002485"
     CDS             complement(25..144)
                     /locus_tag="IWO31_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0302867.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWO31_002486"
     CDS             complement(159..1343)
                     /locus_tag="IWO31_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0302868.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWO31_002487"
     CDS             complement(1599..1853)
                     /locus_tag="IWO31_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302869.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWO31_002488"
     CDS             complement(1878..3020)
                     /locus_tag="IWO31_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0302870.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWO31_002489"
     CDS             complement(3080..4432)
                     /locus_tag="IWO31_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0302871.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWO31_002490"
     CDS             complement(4503..4847)
                     /locus_tag="IWO31_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0302872.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWO31_002491"
     CDS             complement(4857..5231)
                     /locus_tag="IWO31_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0302873.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWO31_002492"
     CDS             complement(5244..5558)
                     /locus_tag="IWO31_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0302874.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWO31_002493"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWO31_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWO31_002494"
     CDS             complement(8976..9638)
                     /locus_tag="IWO31_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302875.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2701 a         2262 c         1710 g         3345 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttc
//
LOCUS       DACECS010000092         9618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.093, whole genome shotgun sequence.
ACCESSION   DACECS010000092 DACECS010000000
VERSION     DACECS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.093"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..1094
                     /locus_tag="IWO31_002495"
     CDS             120..1094
                     /locus_tag="IWO31_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0302876.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1076..1537
                     /locus_tag="IWO31_002496"
     CDS             1076..1537
                     /locus_tag="IWO31_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0302877.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1587..2051
                     /locus_tag="IWO31_002497"
     CDS             1587..2051
                     /locus_tag="IWO31_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0302878.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2065..2814
                     /locus_tag="IWO31_002498"
     CDS             2065..2814
                     /locus_tag="IWO31_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0302879.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2814..3644
                     /locus_tag="IWO31_002499"
     CDS             2814..3644
                     /locus_tag="IWO31_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0302880.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3644..4291
                     /locus_tag="IWO31_002500"
     CDS             3644..4291
                     /locus_tag="IWO31_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0302881.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4389..5165)
                     /locus_tag="IWO31_002501"
     CDS             complement(4389..5165)
                     /locus_tag="IWO31_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0302882.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5309..6268
                     /locus_tag="IWO31_002502"
     CDS             5309..6268
                     /locus_tag="IWO31_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0302883.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6385..6693
                     /locus_tag="IWO31_002503"
     CDS             6385..6693
                     /locus_tag="IWO31_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0302884.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6720..7019
                     /locus_tag="IWO31_002504"
     CDS             6720..7019
                     /locus_tag="IWO31_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0302885.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7193..7744
                     /locus_tag="IWO31_002505"
     CDS             7193..7744
                     /locus_tag="IWO31_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0302886.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7890..8183
                     /locus_tag="IWO31_002506"
     CDS             7890..8183
                     /locus_tag="IWO31_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302887.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8176..8472
                     /locus_tag="IWO31_002507"
     CDS             8176..8472
                     /locus_tag="IWO31_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302888.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8543..9547
                     /locus_tag="IWO31_002508"
     CDS             8543..9547
                     /locus_tag="IWO31_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0302889.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3407 a         1373 c         1797 g         3041 t
ORIGIN      
        1 aaattgttat aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga
       61 taataggtat tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa
      121 tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa
      181 ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat
      241 atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact
      301 atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca
      361 caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt
      421 gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg
      481 attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat
      541 ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg
      601 ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat
      661 ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg
      721 gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg
      781 ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat
      841 tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca
      901 ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta
      961 tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa
     1021 tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc
     1081 aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt
     1141 gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat
     1201 gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt
     1261 tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac
     1321 ggatttaatt ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat
     1381 tcaaattatt gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc
     1441 tggtaaatct atagaagaaa tagattttaa ggaattactg aataactctc aaaaaagtat
     1501 ggttcatatt aattcgcttt taaaagatgt cagttaaggt ggcaatttct attctataaa
     1561 ttttaaaaat tgaaggagga aatagaatga ttaaattagt gcgtgtagac cacagattac
     1621 ttcatggaca agtaatattt tcttggacta aacagtatga tattaaccat attattgtgg
     1681 ctgataacaa agttccagga gatccaatgt ctgtgatggc tttgacaatt gctaaacctg
     1741 ctgattgcaa attagacatt gttaaaatta gtgatgttaa agggattatg gaaacatccc
     1801 caaatgataa ttacatgatt ttaattaaag gacctaagga agcttttgaa ttagttcaaa
     1861 atttaccaga aattaaagaa attaattttg gcggtgtgtc taagaaaagc gagtccaaac
     1921 aatatggaaa ggcagtcttt ttaactcctg aagaattaga agccacaaaa aaaattcttg
     1981 atcaagaagt gaaaatattt gtgcaacaag tcccatcttc aagtattgaa aatgtagatt
     2041 ttagtaagta aggggggaaa ataaatgtta ttaaaagcca ttttattagg tttagttggt
     2101 gtggtagctg ttattgactc caggttaatt ggtcgacaaa atattggtag accactaatt
     2161 ttgagtacac ttgccggact tgtgctaggt gacttgcata cagggattgt tcttggggca
     2221 tctttagaat taatttctat gggattcgtc tcaattgggg cggctggacc tcctaatatg
     2281 caacttggta gtattattgc cactgctttt gcaattttaa ctaatagtag tacagaaaca
     2341 gccttaacaa tagctatccc agtggctgta gctggtgaat ttatgagtat tatcatgcgc
     2401 atgataattg ctcaatttgg acatgctgca gataaagcca ttgaaaataa taaatttaga
     2461 aaagctcaaa ttattcatat ttattggtca tttattttta atgcttttgt ttactttatt
     2521 ccaatctttt taacagtata ttttggtgct gacgttgtgg cagatttagt agataaaatt
     2581 ccagaaatga ttacagacgc cttgactgtc tctggaaata tgctttccgc attagggttt
     2641 gcaatgctat taagcacaat gttgagtaaa aaattatatc cttactttat ttttggattc
     2701 tttattgttg cctattcagg tctttcacta attggcgtga caatttttgc agctctgatt
     2761 gcctttgtaa tggatcaagt taaatatgga aaaagagaag aggcgcatgg ttaatggaaa
     2821 atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct taataagatg tgtcttcgtt
     2881 catggacaat catgtgttcg tggaattatg aaagacaaat gcatatggga tttatgtatg
     2941 ggatggcgcc aattttaaat aaagtttata aaaatgatga agagaaaaaa agagaagctt
     3001 atactcgtca tatggcattt tttaactgta cgcctcaaat gacttcattt attatgggac
     3061 tttgtgcttc tatggaagaa caaaatgctc aagcagaaga aggaaacttt aatactgaat
     3121 ctatctccat gattaaaacc agtttaatgg gtccttttgc tggaattggt gattcatttt
     3181 tccaaggtac tttaagaatt attacatttg ggattggatt gtcttttgct aagcaaggta
     3241 gtattttagg accgatttta gctgttggcc tttttgctat accttctatt ttagctgttt
     3301 tttacggcgg taaattagga tatgagtctg gcaataaata ccttactaaa ttgtatcaag
     3361 aaggtcttat ggatcgagta atgcaatttg cttctatcat tggtttggct gtagtaggtg
     3421 gaatggtggc tagcatggtg ccaattgtca cccctttaaa attcactaca gggggaacag
     3481 aacttgtttt acaagactta cttgattcca ttattcctgg attattacca cttgtatgta
     3541 ctttgggaat atataaattg attaagaaaa atgttagtac aaatatgctc ttattaggtt
     3601 gtattttggc agggtttgtt ttaagtataa taggtatctt ataatgatta aggctctaat
     3661 ttttgatatg gacggagttt taattgatag cgagattatg tatcaaaagt gggtatttaa
     3721 ttttttaaaa caagaaaatt taagctatcc tttgtattct tatcaaaaaa agattggaac
     3781 tacagcaagt atttttgatg atatagaaaa gtacaatgag ggatgtaaca gtttagaatt
     3841 gaagggaaaa tttactaaat attggaaatc gcaaaagatt gattacttgg atatttttcc
     3901 taatatacta attgaacagc ttgaatggtt aaaaaagaaa aaaataaaaa tggcaattgc
     3961 ttcttccagt ccaatggata ctataaatga agttgttacc acttgtaaaa tagagtcata
     4021 ttttgattgt ttaattagcg gaagagattt gccagaaagt aaacctaatc ctactatatt
     4081 ccttaaagct tcagaacagc tcagaattcc agttgaagaa tgtctggtga tagaagattc
     4141 ttataatggt ataaaagctg gtaaaagagc aaatatgaag gttcttgcaa ttaaagataa
     4201 acgtttttct caagatgtaa gtctagcaga tgatgttatt tacgatatta aatatctgcg
     4261 acaagaagta caggtgaaat ttggaatata aataagtttt ttgatgtatt tagagaatat
     4321 tagtaaaaat aaaaatcatg cccaaatcta atcaaaattg attgctttgg gtatgatttc
     4381 ttttaatatc aatctattac tttaggatct gaatcatttt tatgcaccgt atctaaagtg
     4441 atattttctg tgacttcaat aacataacta attctatcac aacgaactgt ggattgagaa
     4501 taatgaaatg gttgattatt tttatctttg ccaacctttt ctaaaataaa tagcggatct
     4561 cctactatac attgcaataa gtcggaaagc tcaggtgttg cagataaacc gtttatttcc
     4621 ataatagagc tcgtagcttt gacatcatag tcatcttcta gaagtttata taatgaagta
     4681 tattggctac ctttttcaaa taaagttgga aatttttctt ctgatgcgaa aaaattatca
     4741 attcccatcg gggcaccgtc ttcccacatt aaacgtttaa tattgtaaac tctatcagtt
     4801 acattgattt gtaatttatt agctaaaaac tcatcagcct ttataatttt agaatgtaaa
     4861 acagtatatc taattttatg tcctcgggta gaaagagaat cgctaaatcc atgaactcca
     4921 gctaatttta aactttcttt tttttgagtt acaaaggtcc ctttccccct taatcgttca
     4981 actgttccac tttctgctaa aagtttaaaa gcttttcgaa tggtaatctt acttactcca
     5041 taaattttac ataattcttc ttctgtaggt aattgctctc ctggaagata ttttttttca
     5101 cgaatatcgt tacgtataga agcttctact tgtaaatata atggtctatt atcttgaaaa
     5161 ttcatagtac actccctaaa aagttatact tcattatatc acaatttatt aaaacgtcga
     5221 ttgtaaaatt aagctagaca actaaaaaga tcatttgtgc tacactcaaa atagttccga
     5281 ccaaagaatt aataaggagt gagtacaaat gacttacacc catcttacaa cagacgaact
     5341 agtaatcata gagtcttatt tcaatatcga gaaacctgtc gctcttgtcg cacaaagttt
     5401 aaatcgctct agacaaacca tttataatgt ttattcgttt ctcaaacaag gaaaaaaagt
     5461 gctagattat taccgtcaat acaaacaaaa taaatctcgt tgtggacgac gttcaaccgt
     5521 tttatctgat gaacaatcaa catatgttca agaaaaggta gctcaaggat ggtcacccga
     5581 tgtgattatc ggccgtggcg agtttccaat tgattgttct gttcgtacac tctatcggat
     5641 gtttaaaaaa ggcctttttg acacacagtc gttacctatg aaggggaaac gtaaaccgaa
     5701 taatcatcaa gaaaagcgtg gaaaacaggc ctttcgcaga acgattcatg aacggaacag
     5761 tctctatcca cacctgacta aagaatttgg tcatctcgaa gtcgacacca ttgtgggagt
     5821 taaacacaag agcgctgtta ttacattggt tgagtgttta tccaaggtta ttattacttt
     5881 gaaaccgaca ggcagacgag ccgaagatat cgaaaacagg ttaaatgatt ggttcaaatc
     5941 catgcctaaa cacttgttta aatccatgac atttgattgt ggaaaagaat tttccaactg
     6001 gcgatctata agtaactcga acgatattgc catctttttc gcagatccag gaacaccttc
     6061 gcaaagaggg ctaaatgagc actcaaacgg cttgcttaga cgagatggct tacctaaaac
     6121 aatggacttt agggacacgg atgaaacctt tattcaatcc gttgcctcga aaagaaacca
     6181 tattccaaga aaatcattaa attatcgaac accattggaa gtatttttga gctgcataga
     6241 taaagacata ttgtctagct taatttgaca aatcagatag tataaaatga atcaaattga
     6301 atatcaaata tatattatat ccatgaagcc caatcttctt taatagaaga ttgggctatt
     6361 tttatggcaa aggagggaaa agaaatgttt caaaataaag gacaacgtta tgtcacaaag
     6421 ggagtaatgg acagtttgcc ggtggaatta caagctttat gttggaatct aatcgatcaa
     6481 aacgttcaaa agcaatttca tttagactat cttcaaatct ttgaattttc tacggaaaaa
     6541 ggaaatcaaa agttggttca tcgtcaagaa gaaccagaag caagaaaaga gtatctgatt
     6601 tcacctaagc ttcgtttgaa atcagtgtct caaaaaatat gggtcattga ttcgggtgag
     6661 tatcaaacga tgttattacc agaagaatac tgatatctaa aagaaaggaa cgaaaggact
     6721 tgttagaaac ggctatcgaa gtactggaaa agtgtgcaca gctagtcact actcctgaag
     6781 agtggggcta tgaatccgtt acgatggaaa aagaggaaat tgaaatgggt atgcttcctg
     6841 aagatgttac tctaccacgt ttagtgatga cacatcttta tatttattgc gcaccagaag
     6901 atggaaaaga ctatgtagtt tatttcatta cagatattac cagccagcga gaatttgttc
     6961 gtgggttact ggtagaagga cgattggttt ggagtcagat tggaggaacg aatgaataag
     7021 atcgattatt tgagaatgat tgcgaaatgt ttagaagata tccttacagc gattcatcaa
     7081 attctgaaga aaaaaggaga aagaatatga aatattttca actagtattg acacttataa
     7141 cggcaatggt gattgaattg cttgaacaaa aattagcata ggagaaaaag agatgattga
     7201 aaaagaatta gtgaccacac aaacagaagt ttggcgagat cctcatcata cccatcgcta
     7261 tgttttcaaa cgacaatggg cgatcaaagg aaaagaagcg gaagataaat tagcggtagt
     7321 cattactatt cgtccaacag atacagaacc atttacgaat gatttgagta tgttattaat
     7381 cgaaaagaat atccgacaat taggatttac tggttttatt gctgtgaatc ttttttcgta
     7441 tacaaaagcg aaaagtcctc gggtatttcc tcgtggaaat gatgaacaat ctttggaaat
     7501 attaacaact gttttcacag aaaagaaaat caacaccatt atttttgctt gtggttcgat
     7561 tacagcgacg agtcaagtgg cttatgaaca agcgaagacc atttacgatc aattgagtgc
     7621 gaaacaaaaa aagatgacca aagtattgat aaatgcagaa ggtaagttat cccacccact
     7681 aagtattcat gttcgtaatg aatggcaatt ggcagatagt caattattat ttcaagaaaa
     7741 ataacatttt attttcttat gttacttata agtagtttta catgcttttt gactttaaaa
     7801 atacatgtga tgatacatgt tatatgaatt atcaatgtac ccagtaatat gctattttca
     7861 ttatgtaaaa aataacaggg ggtgtgtaga tggagattga tgaaacttgt ttgagtaact
     7921 cttataaaag tatgaatgac ttttttgagg aaatggaact ggattcagaa ttgctctatc
     7981 aattttatct tgcttttcga gggcaacaga tttcttttcc aatgaaaatg tacgatcgtg
     8041 agttagttag aaaaagaatc gaaaacatga tagaacagga aaaaactgta gatatccgtc
     8101 aattgacaga agtttatggt ttctctgcaa gatggattca aaaagtaatt aaacaaaaag
     8161 aaaggagacg agtacatggc taagctgatt gtattaggtg gtttaattta cggtggatat
     8221 tggttatata aatatatgct tgcgcatccg aatatagata aaggaaaagt aattaagaca
     8281 gttggggtaa tcatagttgg actcatagct ctaactaaga aaaataatac taatagccca
     8341 aaaataatga gaacttatcg ctgtagagga tgtggaaggg aaatttctac tagttttaat
     8401 ccatctgaaa gccctcaagg aagagatcgt tgtcatgctg gctatcgaca ttcttgggaa
     8461 aggattaact aaatgaacac cattgagatt aattgactga taaaataagt acgcgaggga
     8521 gaaattgaaa gggggaaata aaatgaaaaa atggttgtta ttactaattg gaatggggat
     8581 tttattcact ggttgtacat ctgagaatac gaggactaaa gagactgtag aaactgatac
     8641 aacaattgag gctaaaaaaa gtaaaacgca atcaactgaa caaactacaa cagaaatgaa
     8701 acaatctgtg gaagcaagta caagtgaaag ttcaatcatt gaatcttctt ctgaagaggt
     8761 taaaaatact ttgtgggata gtgagaaatc tactcaacta gcaatttttg tcccacaatg
     8821 gggaaagaca ttaggtcaag aatataaaag ctacaatcct cagaacaatg taagtctgta
     8881 tggtacgcct ttaccaagtg cagttattga tggaaattgg aaaatggcaa tcaatgaagc
     8941 accagttacc gtacaatggt cagaagatgg aactggtcat gcagatttca atttagtagc
     9001 agtttattct gatgtagaaa ctggagaata tttagggcag catgtatatt tctttggctt
     9061 tcaaaatgga caacctaaag tttatttaac gcaacaaaat caaggcaatg ataaaaacta
     9121 catgtacttc aatgaaactc agaaccaaca gttaaaacaa ggtttcattg atattgtgaa
     9181 tggacaaact cctcaaactc cagtagtaga agaatctact caacaagcaa caactacaac
     9241 gacacaatct acaagtagct cagcaatttc aagttgggaa gctgctaaag aaactgtact
     9301 aaataataaa gctttatggt tgtatgattc tcctgataaa aatgcatcaa atactaattt
     9361 aattagcaca aatgaatata tgaaagatct aatgcaagat gaaaatggaa gctactattc
     9421 aattaatatt gtaacggatg ataatggaaa tacaacaatg gctggtggaa aacaattccg
     9481 tgtttataca gatggaagaa tttctcaacg tattggaatg actaacaatt ttcaaatgat
     9541 taaataattg aaaatattat ttatcaaaga aaaactgatt ttgagaaaaa ttatttaatt
     9601 ttcttagaat cagttttt
//
LOCUS       DACECS010000093         9020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.094, whole genome shotgun sequence.
ACCESSION   DACECS010000093 DACECS010000000
VERSION     DACECS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.094"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..614
                     /gene="ubiA"
                     /locus_tag="IWO31_002509"
     CDS             <1..614
                     /gene="ubiA"
                     /locus_tag="IWO31_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HAQ0302890.1"
                     /translation="LLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMV
                     VYVNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLD
                     RDIENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVK
                     KHLDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1428..2774)
                     /gene="gor"
                     /locus_tag="IWO31_002510"
     CDS             complement(1428..2774)
                     /gene="gor"
                     /locus_tag="IWO31_002510"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0302891.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2908..4149)
                     /locus_tag="IWO31_002511"
     CDS             complement(2908..4149)
                     /locus_tag="IWO31_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0302892.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4167..5090)
                     /locus_tag="IWO31_002512"
     CDS             complement(4167..5090)
                     /locus_tag="IWO31_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0302893.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5311..7803)
                     /locus_tag="IWO31_002513"
     CDS             complement(5311..7803)
                     /locus_tag="IWO31_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0302894.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7810..8277)
                     /locus_tag="IWO31_002514"
     CDS             complement(7810..8277)
                     /locus_tag="IWO31_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0302895.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8413..8486)
                     /locus_tag="IWO31_002515"
     tRNA            complement(8413..8486)
                     /locus_tag="IWO31_002515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8450..8452),aa:Arg,seq:acg)
     gene            complement(8497..8582)
                     /locus_tag="IWO31_002516"
     tRNA            complement(8497..8582)
                     /locus_tag="IWO31_002516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8546..8548),aa:Leu,seq:taa)
     gene            complement(8599..8670)
                     /locus_tag="IWO31_002517"
     tRNA            complement(8599..8670)
                     /locus_tag="IWO31_002517"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8636..8638),aa:Gly,seq:gcc)
     gene            complement(8682..8754)
                     /locus_tag="IWO31_002518"
     tRNA            complement(8682..8754)
                     /locus_tag="IWO31_002518"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8719..8721),aa:Thr,seq:tgt)
     gene            complement(8772..8853)
                     /locus_tag="IWO31_002519"
     tRNA            complement(8772..8853)
                     /locus_tag="IWO31_002519"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8817..8819),aa:Leu,seq:tag)
     gene            complement(8861..8933)
                     /locus_tag="IWO31_002520"
     tRNA            complement(8861..8933)
                     /locus_tag="IWO31_002520"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8898..8900),aa:Lys,seq:ttt)
     gene            complement(8937..9009)
                     /locus_tag="IWO31_002521"
     tRNA            complement(8937..9009)
                     /locus_tag="IWO31_002521"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8974..8976),aa:Val,seq:tac)
BASE COUNT         2462 a         1966 c         1535 g         3057 t
ORIGIN      
        1 ttttactttt gatgggattt atctgttgtt ttattggagt attctataca ttcggaccga
       61 ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg ggccttggaa
      121 tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat ctgacagtca
      181 acttttcatc tggacaattt gatttggtag gaaatctttg ggctatagca gcaatcgtat
      241 tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat aatttgcggg
      301 atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc ggacgtcctg
      361 cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt ttaatggggt
      421 tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg ccaattgtga
      481 agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc agctattcca
      541 tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta acaatcatta
      601 tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc aagtaataag
      661 aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat taccgcagat
      721 gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt ttcgagtatt
      781 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgag
      841 aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg tcctgagtat
      901 tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
      961 ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca caattttttt
     1021 cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag aactttcttg
     1081 gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa tttatttata
     1141 caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt ttaaacatcg
     1201 ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc ttcgtcagtt
     1261 acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat tatcgaagca
     1321 atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga ttttctggtt
     1381 ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta tcgcatcgtt
     1441 acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc ttctttagtt
     1501 gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc aacaccaata
     1561 gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat ttcgcatttt
     1621 tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata aactttcaat
     1681 tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt tgcaattggt
     1741 ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc tttctttcca
     1801 ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt cagatctaat
     1861 ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg gtatttatct
     1921 actttgataa accctttttt gtccaattcc acatttgtat tttccagacc taatttttcg
     1981 gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt ttcaccattt
     2041 tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat atgatgtgcg
     2101 taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag cattttatca
     2161 aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc taaaccacga
     2221 aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc tctcttaggt
     2281 aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc aggaatggtc
     2341 attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata ggtttcaccg
     2401 tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc ctcgacatga
     2461 ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt ttctcgtctt
     2521 tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta acttgaggca
     2581 tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt aggtacacaa
     2641 ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca
     2701 tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat tacgatgtaa
     2761 tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct ttatttaacg
     2821 atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg cgaagatgct
     2881 taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat ctccaggttt
     2941 caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc ggtcaatgga
     3001 ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga atcctggacc
     3061 ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg tagcaatctg
     3121 tgttgcctca tcataagata atacttcacc gacaaattta tatactggga ttttacgacg
     3181 ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg tagctaattc
     3241 tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat agttttcagg
     3301 atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag atacataatg
     3361 gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg
     3421 aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt ccacttcgcc
     3481 tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc catacttcca
     3541 gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat ggttggacaa
     3601 tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt cgatttcggt
     3661 atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag ctaaaacgac
     3721 acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg ctgaagcttg
     3781 agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga tcaaagctgg
     3841 atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa attcccctgc
     3901 tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct tcgcatcatg
     3961 ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac ctgcacggct
     4021 tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat ggatcgcggt
     4081 ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca attttctttt
     4141 tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat ataaaagaat
     4201 cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt cattttttcc
     4261 gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc gaatagtcga
     4321 gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca tgtacagtaa
     4381 tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct ggcaaaaata
     4441 tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt aggttccgag
     4501 ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt atagtaattc
     4561 tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag ggtttctaca
     4621 ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc acgagcaagt
     4681 actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct cgttactaat
     4741 gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat cgtataaata
     4801 atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa agccaaatat
     4861 tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat attcgctttt
     4921 ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca
     4981 ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc
     5041 aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat aattttctcc
     5101 tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc ttctatttaa
     5161 agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata tattctgtcc
     5221 aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa gtcttactat
     5281 tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt cctttttagg
     5341 tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt ggtcacctaa
     5401 acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt tttgaagtgc
     5461 gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga tcacatcgat
     5521 tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga taatcgcttt
     5581 gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga agacgacagt
     5641 ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt ctaagatacg
     5701 tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga catttttttc
     5761 ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat ttctaaagtc
     5821 cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca gaagtaaatt
     5881 gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat atggtttttg
     5941 acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg gaggagaccc
     6001 gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac ggatcagggc
     6061 atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt ttccgacacc
     6121 agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca atccgcttcg
     6181 tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa cgacacgttc
     6241 atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca actgctgtag
     6301 agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta cacgatcaga
     6361 ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt tacgtagtgc
     6421 tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc
     6481 cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg ttaagtgatc
     6541 ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg ctttatcggg
     6601 tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta cagcttcatc
     6661 tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa gaatcacttc
     6721 tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct ctaatgcaga
     6781 atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg tttgtaattc
     6841 tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt ctgctccacc
     6901 tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat cattatagat
     6961 ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg taccagcaac
     7021 caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg gcacttcacc
     7081 taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac caggttcacc
     7141 caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc gtttcacttc
     7201 tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg tcaaatcacg
     7261 tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt gtacatttac
     7321 atttctacga cgattcattc cttttccagc agaatcacca gctagcccca ttttttgttt
     7381 cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc gagtagccat
     7441 gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt tttgagaacc
     7501 agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg
     7561 aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat gttcaatttc
     7621 ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag caattccatt
     7681 ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat gtttcaaccg
     7741 ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa aaagttcttc
     7801 catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc ggagaatccg
     7861 agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa ttgcactcaa
     7921 catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt gaataatagc
     7981 aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct caagtaattg
     8041 ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac cacgtttgct
     8101 ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt tgatttgtga
     8161 aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca tttcactatt
     8221 ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat gtgccatctt
     8281 attcattccc tttgactaat attgacctta attataacga atttgataat agaaagaaag
     8341 aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac ggaatatact
     8401 attccgtatc gacgcgccca agaggagtcg aacccctaac cttttgatcc gtagtcaaac
     8461 actctatcca gttgagctat gggcgcatat ttgaaatgcc gaggaccgga atcgaaccgg
     8521 tacggtgatc actcaccgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
     8581 gcgcgattgc tttggcaaag cggaaaacgg ggttcgaacc cgcgaccccc accttggcaa
     8641 ggtggtgctc taccactgag ctatttccgc gtgtttaaag atgccggcta aaggacttga
     8701 accctcgacc ctctgattac aaatcagatg ctctaccaac tgagctaagc cggcatgtaa
     8761 aacttaaatt atgcgggtga agggacttga acccccacgc cgtaaggcgc tagatcctaa
     8821 atctagtgcg tctgccaatt ccgccacacc cgcaaaaata tgagccgtac agggctcgaa
     8881 cctgtgaccc tctgattaaa agtcagatgc tctaccaact gagctaacgg ctcatatgga
     8941 ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc ccagctgagc
     9001 taaacctcca tggataactt
//
LOCUS       DACECS010000094         8904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.095, whole genome shotgun sequence.
ACCESSION   DACECS010000094 DACECS010000000
VERSION     DACECS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.095"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(305..661)
                     /locus_tag="IWO31_002522"
     CDS             complement(305..661)
                     /locus_tag="IWO31_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0302896.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="IWO31_002523"
     CDS             complement(675..1085)
                     /locus_tag="IWO31_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0302897.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="IWO31_002524"
     CDS             complement(1432..3618)
                     /locus_tag="IWO31_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0302898.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="IWO31_002525"
     CDS             3836..4519
                     /locus_tag="IWO31_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0302899.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="IWO31_002526"
     CDS             complement(4600..5319)
                     /locus_tag="IWO31_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0302900.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="IWO31_002527"
     CDS             complement(5631..5837)
                     /locus_tag="IWO31_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302901.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="IWO31_002528"
     CDS             6082..6684
                     /locus_tag="IWO31_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0302902.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="IWO31_002529"
     CDS             6825..8105
                     /locus_tag="IWO31_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0302903.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="IWO31_002530"
     CDS             8118..8846
                     /locus_tag="IWO31_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0302904.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DACECS010000095         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.096, whole genome shotgun sequence.
ACCESSION   DACECS010000095 DACECS010000000
VERSION     DACECS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.096"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWO31_002531"
     CDS             complement(<1..173)
                     /locus_tag="IWO31_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0302905.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWO31_002532"
     CDS             complement(593..1714)
                     /locus_tag="IWO31_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0302906.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWO31_002533"
     CDS             2042..3451
                     /locus_tag="IWO31_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0302907.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWO31_002534"
     CDS             3448..4176
                     /locus_tag="IWO31_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0302908.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWO31_002535"
     CDS             complement(4304..5215)
                     /locus_tag="IWO31_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302909.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWO31_002536"
     CDS             complement(5232..6239)
                     /locus_tag="IWO31_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0302910.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWO31_002537"
     CDS             complement(6236..8365)
                     /locus_tag="IWO31_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0302911.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWO31_002538"
     CDS             complement(8370..8477)
                     /locus_tag="IWO31_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302912.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1487 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga gacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACECS010000096         8112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.097, whole genome shotgun sequence.
ACCESSION   DACECS010000096 DACECS010000000
VERSION     DACECS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.097"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..110)
                     /locus_tag="IWO31_002539"
     CDS             complement(<1..110)
                     /locus_tag="IWO31_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0302913.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
     gene            complement(179..385)
                     /locus_tag="IWO31_002540"
     CDS             complement(179..385)
                     /locus_tag="IWO31_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302914.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
     gene            complement(548..2182)
                     /locus_tag="IWO31_002541"
     CDS             complement(548..2182)
                     /locus_tag="IWO31_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0302915.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2186..3559)
                     /locus_tag="IWO31_002542"
     CDS             complement(2186..3559)
                     /locus_tag="IWO31_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0302916.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3575..3814)
                     /locus_tag="IWO31_002543"
     CDS             complement(3575..3814)
                     /locus_tag="IWO31_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302917.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3996..6671)
                     /locus_tag="IWO31_002544"
     CDS             complement(3996..6671)
                     /locus_tag="IWO31_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAQ0302918.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6854..7777)
                     /locus_tag="IWO31_002545"
     CDS             complement(6854..7777)
                     /locus_tag="IWO31_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302919.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2373 a         1595 c         1228 g         2916 t
ORIGIN      
        1 gttagttcat attctcgaat gatttctaca cgtggctttc cagctaagta aagattgacg
       61 atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat aaaaattcct
      121 cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta attgtagcct
      181 atccagtata ataaacattt ccttgaggtt tccttttaat ccccttcgga aaatcacctt
      241 ttgtaaaatc gctcttaagg aaatcatcta aattaccagt tctggtcttt ttacctccag
      301 tattccctga ttttttaggt ggtacatgtt ctttagtaag ttcagtgtat ttttcacata
      361 aaatacactt acccatcttt ttcatatcta tccccactct ttttaattct tatatacaat
      421 tatatttcaa atattcatta ataaatatcc ctatttatga taaggacctc cctaactact
      481 tctctaacga tttcaaatcg ctagagaagc gttagcgccc ctctaacgtt gtttgatata
      541 acctagacta gatcgttaac gtaaagggtg tttttaacac aaaaatagcc gtatcctttg
      601 ttttggatac ggctatttga tattgctcat tcaattgttg taccacggca tgcgtgaatt
      661 tcatcgtaat ttccaacatc tccttatggt taggatcaaa ggttattttc tctataaagg
      721 tacgatacaa ggctttgatt tgcttcttct cactctgggc aagaaaacga tccaaattag
      781 tcaatatccg ttggacatct ttgacttgaa tcggctctcc ttgttgaaag atccctaaga
      841 tttcattttc tcgaatgtgc aactgtcgtc gtttttcagt caattcgtct aagcggtctt
      901 ttaacatcgg aaacaattca ggactatctt ccaaagcagc ttcccatttt tcaattttag
      961 tcttcaattc ttcctttcgt tccagtaaca ccacgagttc ttgttccaat ggttggcttt
     1021 gtcgcactat ttcttcattt aacgctgcca ccaatcgggg aagaacttct ggtagctgaa
     1081 cgatctcctt taatcggtcg gcgacaaatg tttccgcttg ttcggcacga atcgaattgg
     1141 catgacacac acttgctcct ttattccgaa agactgaaca agaataatat cgaatccgtt
     1201 tctttgtgcc atcctttagg gtatttgtga cattactcgc tgccatcggt gcgccacatt
     1261 ctggacaacg taacaagcct gtcaatacat tttcacctgt atggttccat ttgggttgtt
     1321 ttccttctaa agcaaggcgt tcttgtactt tttgataatc ttcttccgaa ataatcggtt
     1381 catgggttcc tttcactaaa atcggtctcg gattcttccc cttccgtcgt ttggtttccc
     1441 aattgatgta gcgcgcatat tctaaatacc ctgcataaat tttattgcgt aagatatctt
     1501 tgactgccgt ggtagaaaaa gcattctttt tgactgtttg atatccttga cgattcaaaa
     1561 aattggcaat cgctcgatac cctgctccgc ctaaatattt tgcatagatc agacgaacaa
     1621 ttttcgcctc ttctggttca atgaccaatt gtttttgacc attcgtgtca ataccactga
     1681 catagcctaa acgtcggcct cctgcataac gctttttatc tttcactaag ctttgcattg
     1741 acatttgaac attctccgca atttggttgc gttcaaattc accgaaactc ccaaggagtt
     1801 gtaacatcaa tttacctgtc gaagtggtca cgtcaaattg ttccgagatc gaatgtaatc
     1861 ctacgtcata tcgcaataat tcctcgactg tttgtaagac atctaacata ttgcgtccga
     1921 gacgggtcaa tttccaaatc actagtagat caaaacgatg gttccgagca tcgttcatca
     1981 ttcgttgata ggcgaaccga ttttttgtgg atttcccact gattccttca tcggcataaa
     2041 cttctaccac ttcatagccc atccgttcgg caaactgtcg tccattttgt tcttgtgctt
     2101 gaatactatg tccttctaaa gcttgttctt gtgtactcac gcgaatatac aatgcgactc
     2161 gtttggtttg tatcatttcc atagatcatt cgttcctttc taaaatattt tgtgtcagtt
     2221 gtcggaagta aatcccacaa actgttttat cctttgttac ctcaatccga caaacatagt
     2281 cacataataa ttgtcgacta gagaggtctt ttagttgaag ccattctttc acttttgctt
     2341 cacactgttg gagtgaaaga aacgaagaag tagacatcgg cttttgaaga gcttgcagtt
     2401 gttgcttgag ttcttccagt gaacaattgc cctcttcaaa tgccttaatc cattgtgctt
     2461 cttgtttctt ttgttggatt actttgcgtt tttcatcttt tcgcttttcg gctaattgtt
     2521 gcgttaattg ggcaatcaag gcttgataga cgccttcgta ttgaaaaaaa gcctgtatct
     2581 tttccaatac ttctgactca accctttctg cccgaagaaa aattccttgg cagcttcggt
     2641 cggtacaatg ataataatga agcgtcttcg tcttctttgt attccattga tacttcgaac
     2701 tcagatgacg accacagaag ggacaccgaa ttttttgacg taataagtac agatacgtat
     2761 cttttttagc tgtacacctt gattggcgaa tctcttgggc ttgtaaaaag gttgctttcg
     2821 agagaatcgg ttcaaaggtt cctagatact tccccaaaga tcctcctttg atttcgccat
     2881 agtacaatgg attttttagg atactataaa tatgataggg ctgaaaagga cgttcgcgga
     2941 agaaaagctt ttgttgcgtc aatcgttgac aaagcttttt gtagccatat tttttggtca
     3001 aataaagttg aaaaatatag cgaacaatcc ttgcttcgtg tacttcttgt tggattttcc
     3061 cattcactaa acgataccca tatggtaccg cagaagacaa atattgccgt tgttgctttt
     3121 tataggaaag tccggcacga acattctcaa taatgacgtt tcgttgttgt tctgcaaaag
     3181 tgaacaataa ttgcacaaat agtttattcg ttgacgcgtg tgagaaagaa gcaggaagag
     3241 gttcagaaat cgaatacact tgaacctgat gttgttggca caactcaata aattctaagg
     3301 ctattttggc ttgacgtgcc aatcgatcat aacgccaaac atagatcacc ccctcttctt
     3361 ctgacgcaca aacccaagct ctaagctgtc gaaaagctgg acgctccgct ttttcccctg
     3421 attctccttt gtctgaaaat cgttggggaa tttgttcata tttctctaaa acatactctt
     3481 tcactgcatc atattgagcg tcattactaa ttttcgcttc ttcataaaca gacgttcgat
     3541 cataacaagc cacacgcatg cttgattccc ctctctatga atgaactaaa taccattttt
     3601 tcacgactct tctattgaac gaacgttgca caaagtgatt ttctaaatat ccttcgttct
     3661 tcaacgcttg aagagtaccc ccgatagatc cagcagatac ccctcttatt tcgaaatcca
     3721 agtaaattgg ttttgtaaaa aaggcttgat ttgaaaaatg ttcttgaata tacagaagaa
     3781 cctctgcttt tagtgtcatt ttatttttag tcattagaat tttcctttct ttcatgtttg
     3841 attgaatgaa gtaaatgaag catagatcaa gcttcattta cttccgtttg ggtttgctcg
     3901 ctatcataca gataagtaat cagttgtttt gctaaatcaa taagaaatat ttccataaca
     3961 tggtgcctca ctttcatact ttggtttatt ctaaattaca atcgaaaata cgatttgagt
     4021 gaagaaggta atagaatctc gtatgttgga agaacttgac gattcatgtg aatacgtttc
     4081 gttcttctcc ctttttctgc acacatatta cccgaatcaa gcaattgcct aaaaagatag
     4141 gttagatcct ttacttgaaa ttctcgcctt aacaagtcct caaacgggat aggaaaaata
     4201 tttaccttaa tatccccatt tttttgaatt gatacttttc cccaaatcgt actgggcgta
     4261 tgtttgttgt cactgtttgt taaaaattta tggctattca tcaccaacaa tgttttcact
     4321 cggtcaaata ccgtttgctc acttcctaaa ctagtatcta caattaactg ttcgtacgaa
     4381 ttaataagca ccgtctgtat atgctctaat cgtaaaggat agcccagtat atcttgaatt
     4441 aggagggctc cactataaat caaagctaga ttattaccaa tgcgttgctt caaagaatgt
     4501 tcaggtaaaa cagcccatag ttttttttga gcttcgacaa aagttgcttc aatttttgta
     4561 atcccttgtt caaagagttt caaaagaaat aactttatac cccaaccaaa agtattagaa
     4621 gaaagacttt taactttctc tgcgtgattc gcatcctttg taaacttgat atcagaaaat
     4681 tcgatatatc gaactttcaa accagttaat ttctcattga aataattttc caaacgctct
     4741 tcagaagagg taagggtcaa aacagaaaaa gtctcttgtg gcataagctg acaattagaa
     4801 tctaatctta gcctttcttc tcctgaagct aattgataga tcaacgagct aaagttattt
     4861 tgagttgctg ctcctaactc atctagtatt agtggaacac ctccctgatt gtttgatgct
     4921 agctttataa tggctttatt ggtagcattc atatttctaa gaaccgtagt tggatttcca
     4981 aataaagagg caataaagtg taacgtagtg gttttacccg ttgtgctctt gccctgtagg
     5041 ttaattagta aactcaaaaa agtattagcc ttcgtaacgt ttaaatagcc taacacaata
     5101 gaagcaatac caatcgacca agcaagttct aagttcgttc ctaaaacata cttatgaacc
     5161 aatgcgtcaa attccgcttc tgtaccatta gttgttaatt ggtagcatga gtttttagtt
     5221 aatacagaag gaatttcacg tccaaaagaa tcttgtaact gaaaaatcat tgcttcattt
     5281 tcttttttcc agccaagaga ttcatgttca aaaaagattg gagcattctc ccgttgaatc
     5341 tgtaaaaagt caacgacttc agcttctctt ctagctttaa tgattaagcc atgttttgct
     5401 aattgtttaa aattattagt ttgtaaacat ccaccttcta tttctatttc atcccaacaa
     5461 tgatcgacat aataggcaag aacaagtgta atgacttttt gttcgatatt tttgcgtatt
     5521 tgcttaataa acatcggaca agcaactttt tcttcttgac cttctttcac caaaaatact
     5581 ccttcttgat tcagtttaaa ctggtcaaaa taatattctt gattggattc taatgattct
     5641 acagaaggat ttacaggttt tggcaacgga tattttttta caaagtcttg aatatcttta
     5701 tttttatccg aagcatgcag acaatgaaat cgaatcttat tatcagccgt aacagacaaa
     5761 caagaaccat tagaattatt cgtgtgtttt cttaatggac attcgtcaaa tataagtaag
     5821 gtaactcctt tatgatcgcc ctcttttatg gtataagtta attcaggata gtgagctaac
     5881 cattgtttat agtctgcttg aataaaaggt aacacttctc taaaagatgg atggcttttt
     5941 actctagagg ttgacatcat ggaattttct tcaactatct gttcaagaat tttaaaacaa
     6001 ttacttctat ctatgttatc gggaatagaa aggaaactag ataaacgatg cggacgctct
     6061 tttgtggact ttcctttgac cgctggtgta ccaactaatt tgcataatct tgaaggatta
     6121 taaacagaag tatcaatatc aacttcatct tctactccaa atcttcggtg aagtaattga
     6181 agaaaattct gaatagtaag aatcgtctcg tgattatttg gttgttcatt tatgggaaga
     6241 aaaatatggt agccattccc actatcaact tttacaaatg attctattct cttttctttc
     6301 aaataacgtt ctacttgttc cataacattt tctgcctttt tcttttcgct atctgttgca
     6361 cttccctctt tccttttagg gtcaatatca ataaatacaa accgaatttt ttcaatatca
     6421 ttgtctctca cagaatgctt tttttgtgta atcacctctt gtttaatagg attataagtt
     6481 atgtttactg taccttcaga caatttctgt ttttctaaat agttaataat tgcttgagaa
     6541 tctgcagtag aaaaaatatg agaaacggtt ttcttgttta atgtagaaaa agttctcaaa
     6601 ttaacataag aaatccccag tgtatttaac agttgtaaat gcttctgaat tgaagtgtga
     6661 ttcattttca ttaattacca ctcactttct aaaaaatttt tatttgtatg actacattac
     6721 ctccaacctc tcataatttc atgatcttca gttctacaaa aatatatccc actcaaaatc
     6781 ttgacaaaag aaaaaagttg tgattttgga aatcacaact tttttctttt acgataaatc
     6841 aatccttatt aaattattta ggaaaatatt gttcaattat ttctttatat attttctcat
     6901 agtatgtttc tgtccatcct aatgttccta taaaatcaag tcctctacat tttaaaggtt
     6961 cacttaaact attaatatag ttaataatgt tgtctatcct tttctcttta tcttcacctt
     7021 caatctgggg agcatatagt ccaatttcct cccaataata tgaatctctt tcaattccca
     7081 tgatggtagc tttggctaat ttcaaatcaa ataacgattt tagaattctt agtgtctcaa
     7141 taaagtcttc tctcttcaac tctttaatcc attgtgaatt ttctggatta tctaaaattt
     7201 caagttcatc ttcattataa gtatatttca aatttgactt gcttaactca tagataaatg
     7261 aagtttcttg actcataatt cttttaagta cttgactata agtttcgttt gactttgccc
     7321 atttcgctaa ttctaccact gtattcacta ataaatcgaa ataatgatgt tctcgttcaa
     7381 tagtctcagc attttgaaaa tgaaaatcac ctttataaac tccattcttt tccactgttt
     7441 tattaatatt ttttaaaaac cagaatgagt ttgcaaattt agtcgtactt tttcgattaa
     7501 ctaagttaat aactcttcca tgtaataata agacaaaaat acataaattt ttagcttcct
     7561 tattattaga tactaattga tcaatcatag ggagccattt tttgattttc actaattgat
     7621 tgctcataaa tctttcactg gacttttgtt caatcttaaa ttgcaaaact tctaactcat
     7681 aattcccatc ttctgattta tttttgggaa caactatcaa acttttataa agtttatcca
     7741 catcaaaatt tatctttttt gcataatttg tagccattaa tacatctcct tattcataat
     7801 aaatttcata tgacgctcct ctcaaaattt accggtagac tagaaaaaag aataaaagat
     7861 acgtcaattc acactttatt cttaaaaaaa aactggtctc tctaccggta atatcgattg
     7921 agtccgcctt tatcgacttt atcctttttt tatattaaat tttttcaccg gtagacaggg
     7981 agagaaagaa taaactaaaa agcccttaat tcttaatgtt ttcacctcct taccgttcct
     8041 accgctaaaa tttaatataa ctaaattcta taacattaga agaaatttta aatacctttg
     8101 ataaaaaata tt
//
LOCUS       DACECS010000097         8003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.098, whole genome shotgun sequence.
ACCESSION   DACECS010000097 DACECS010000000
VERSION     DACECS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.098"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWO31_002546"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWO31_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0302920.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3320)
                     /locus_tag="IWO31_002547"
     CDS             complement(2886..3320)
                     /locus_tag="IWO31_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302921.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3337..4542)
                     /locus_tag="IWO31_002548"
     CDS             complement(3337..4542)
                     /locus_tag="IWO31_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0302922.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5236..5622
                     /locus_tag="IWO31_002549"
     CDS             5236..5622
                     /locus_tag="IWO31_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0302923.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5716..5907
                     /locus_tag="IWO31_002550"
     CDS             5716..5907
                     /locus_tag="IWO31_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0302924.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6421..6753
                     /locus_tag="IWO31_002551"
     CDS             6421..6753
                     /locus_tag="IWO31_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0302925.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7049..7204
                     /locus_tag="IWO31_002552"
     CDS             7049..7204
                     /locus_tag="IWO31_002552"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0302926.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7480..8003
                     /locus_tag="IWO31_002553"
                     /pseudo
     CDS             7480..8003
                     /locus_tag="IWO31_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2657 a         1428 c         1171 g         2747 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagtg tttgcatcaa cacttgcatt accgaagtac ttattataga
      181 catctgaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcgataagtt tagttaaatc attattagtt attgaaccta
     1141 tgccataatt ttcatgacct tcccgtcgct cgattgctaa atcagctact tttgttccgg
     1201 attcaaacga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagatga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggtttc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacccggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta ggctcttttc aaacgtaatt accccattag
     1681 gagcggtttg cgtatcggtt gtttctgccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggc
     2101 tgcttggaga aatttcatct tgtgctttaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaatcaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa taattacttt cctagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa ccttatgtta actaatttac ttagagaatt tcatgaacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ccctttctta gattaaactt cagagtattc
     2581 aaataacgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcatttctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt atcaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgtttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaaa ttttttgtta aaatccaatt tcctcaactc ctccaaacta
     3301 cgtaaatgat atagtctcat aataaaataa aacctctcag ctaatgttgt tgaaattgtc
     3361 cactaaacct aattgatatt gcttaggaga tacacctgcg tactttttga atactctcga
     3421 aaagtgtgaa gaatctgaaa atcctaaacc taaagaaact tcactgatag tatggtttga
     3481 tttcaacatt attttagccg tagaaatttt tcgcttagag atataattat aaatcgtaat
     3541 atgcatttca ttcttaaagc tttctcttaa tttagtttca cttacagaaa aataattagc
     3601 tatttccttt acagtaattc ttttatagat atttaagccg atgaattgaa cgacagaaga
     3661 aattaaggga gaaagatgct ctatcttagt ttttccgatt tccttagtat aataaacaat
     3721 cccagaatcc ctcactttta agacctcagg taacgataca gctaattcgt tttcttgtat
     3781 caaagcatct ctactttgat acgcttctat tatatccatt cctaatgtta tagacgtttg
     3841 tgctaacttt tcaaaaacaa tgatagaata atttttttca gaccgaatcg tatctccact
     3901 aacacttgga attattccat tactcagatt aaaaaccatg ttttctaaat ttcgaatatc
     3961 tccattttta acatattcca gtattttatt ttcataaaga aacagatata tttctggatc
     4021 gtaattttgt gataacagat tatctatttt gatttgctcg atagttttac ttagattttc
     4081 tgtatattca attaacggtt catggaatag atcttctatt tttccagtaa aaaaataatg
     4141 aactaaaatc agaatatctc gaatgtctcc taaagaatac aaagtcaaat tttgcaatga
     4201 attatagagt cgttctctat cagccattgc aacattcaaa ttgttcattt ttagtttaaa
     4261 aaaaacttta tcaatattat ttgctctaaa tggaccaaat aagtaatatc tggttttaaa
     4321 catgtataga agaaacatct cattaaaatc accagtaaag aaatggaaat ctttagtttt
     4381 tttaatatta tcagttagta actttttata atcataaaaa aaataagtag ttctatctga
     4441 gcgatactct tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca
     4501 aacatgaaga gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc
     4561 tttctacatc attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat
     4621 aagctaactt acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa
     4681 aaagtacatt ttatcaccga gaactcatca tttttcatca tgatgaatac tatttacata
     4741 tatctagttc aaagacaata attattatga tccgttatta tctaagcact atacagataa
     4801 taggaatttt tcgtttctaa atattccaat ttgggaatcc aataaaaatt ttcgttcaaa
     4861 tacgtgtcta ttcgaactct tttctagttg ttaattatga agggggaaga aataaaaaat
     4921 acaatctata cacgaataag aatattatcc aaaaggaggt tttagaaaag atttattata
     4981 cgtaaaaagc tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat
     5041 atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt
     5101 gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc
     5161 agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg
     5221 gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc
     5281 tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa
     5341 aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt
     5401 aaacaaccga ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa
     5461 atccttcctt tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat
     5521 aaaactgata aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta
     5581 aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa
     5641 ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg
     5701 gaagaaggta gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat
     5761 gaagaagctg ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg
     5821 agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct
     5881 atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa
     5941 gatagtttca aatttcgaac aataaatttg aaactatctt ctttataatt ataaagtatc
     6001 gattttacag cgttctataa tgattcatag aatctaaaac gaatgatttt aattatcaac
     6061 gttttttaat tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta
     6121 ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat ggtacttcaa
     6181 cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat
     6241 ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt
     6301 ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa
     6361 gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta
     6421 ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa
     6481 aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga
     6541 tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta
     6601 cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac
     6661 gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg
     6721 atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat
     6781 gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa
     6841 tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgat
     6901 ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt
     6961 agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag agattgtcgt
     7021 ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag
     7081 ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca cacattcagt
     7141 gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa
     7201 atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca
     7261 agattattta gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc
     7321 ttgacaattt gaagcgaaga aaataaggga tctataatat ctagtgttgg tgacgccttc
     7381 tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttaa aatcctttag
     7441 aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc
     7501 aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc ctttcgcaaa
     7561 gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccggag
     7621 gaatgctttc atcgccacta tcaaaatgaa caaacgatca ttaaataggg ctggaaaaaa
     7681 gtatccatct tattgaatga tgtcagtaac tacaagacca ttctgcggat taacaaacag
     7741 cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt tagtgatcgt
     7801 cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag cgaacctctt
     7861 tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat tcgaatactt
     7921 cgcagccttc ggcctaaaac catttctttg agccagctgt taccagaagt ggtgtgtttc
     7981 gatgaattca aatcagtgaa aaa
//
LOCUS       DACECS010000098         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.099, whole genome shotgun sequence.
ACCESSION   DACECS010000098 DACECS010000000
VERSION     DACECS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.099"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWO31_002554"
     CDS             complement(138..428)
                     /locus_tag="IWO31_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302927.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWO31_002555"
     CDS             complement(546..806)
                     /locus_tag="IWO31_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302928.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWO31_002556"
     CDS             complement(943..1356)
                     /locus_tag="IWO31_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0302929.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWO31_002557"
     CDS             complement(1812..2297)
                     /locus_tag="IWO31_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302930.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWO31_002558"
     CDS             complement(2436..2651)
                     /locus_tag="IWO31_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0302931.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWO31_002559"
     CDS             2875..3675
                     /locus_tag="IWO31_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0302932.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWO31_002560"
     CDS             3694..5562
                     /locus_tag="IWO31_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0302933.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWO31_002561"
     CDS             5555..6046
                     /locus_tag="IWO31_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0302934.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWO31_002562"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWO31_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0302935.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWO31_002563"
     CDS             6606..>7600
                     /locus_tag="IWO31_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0302936.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACECS010000099         7486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.100, whole genome shotgun sequence.
ACCESSION   DACECS010000099 DACECS010000000
VERSION     DACECS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.100"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..2107
                     /locus_tag="IWO31_002564"
     CDS             179..2107
                     /locus_tag="IWO31_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0302937.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="IWO31_002565"
     CDS             2245..2700
                     /locus_tag="IWO31_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0302938.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="IWO31_002566"
     CDS             3170..3439
                     /locus_tag="IWO31_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302939.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="IWO31_002567"
     CDS             3839..4093
                     /locus_tag="IWO31_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0302940.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="IWO31_002568"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="IWO31_002568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0302941.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7479)
                     /locus_tag="IWO31_002569"
     CDS             complement(5266..7479)
                     /locus_tag="IWO31_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0302942.1"
                     /translation="MIICLLYYTDNWGYRLLLAGIFLWFSLKEGRFIAYLCILCSITV
                     SISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTMEGRLSENKQKLQLSYQVNTEA
                     ELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAYFRNHRISGRFQIQKIEQITM
                     QNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGYKDAGFREPEEIFRESGLLHL
                     FSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTICYVSIAGSSISVLRASLLFL
                     LRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGGQLSFFMSFLFILISFDGDWL
                     KKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPVFKFLFLPGVLFLTLFNRWLP
                     ETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGMYIFGLLICMDRLKEQPVKHL
                     FYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSPFRKETILIDTGGQLHFEREK
                     WAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDHVGELPTLSQHFTIKKIYIGW
                     GAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLYVLTPFEKGEGRNEDSIGLWM
                     EYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHHGSRTSSNTDFLSHIEAKHGI
                     ISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITYRWHPVFHPHGLVETMID"
BASE COUNT         2392 a         1417 c         1307 g         2370 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgta
//
LOCUS       DACECS010000100         7408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.101, whole genome shotgun sequence.
ACCESSION   DACECS010000100 DACECS010000000
VERSION     DACECS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.101"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..348)
                     /locus_tag="IWO31_002570"
     CDS             complement(13..348)
                     /locus_tag="IWO31_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0302943.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..604)
                     /locus_tag="IWO31_002571"
     CDS             complement(332..604)
                     /locus_tag="IWO31_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ0302944.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="IWO31_002572"
     CDS             complement(1055..1858)
                     /locus_tag="IWO31_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0302945.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="IWO31_002573"
     CDS             complement(1836..2960)
                     /locus_tag="IWO31_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0302946.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="IWO31_002574"
     CDS             complement(2976..3791)
                     /locus_tag="IWO31_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0302947.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4662)
                     /locus_tag="IWO31_002575"
     CDS             complement(3781..4662)
                     /locus_tag="IWO31_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0302948.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="IWO31_002576"
     CDS             complement(4738..6027)
                     /locus_tag="IWO31_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0302949.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6255..6899)
                     /locus_tag="IWO31_002577"
                     /pseudo
     CDS             complement(6255..6899)
                     /locus_tag="IWO31_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1589 c         1136 g         2405 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DACECS010000101         7161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.102, whole genome shotgun sequence.
ACCESSION   DACECS010000101 DACECS010000000
VERSION     DACECS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.102"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..181)
                     /locus_tag="IWO31_002578"
     CDS             complement(<1..181)
                     /locus_tag="IWO31_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0302950.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINN"
     gene            complement(194..1297)
                     /locus_tag="IWO31_002579"
     CDS             complement(194..1297)
                     /locus_tag="IWO31_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0302951.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1387..2322)
                     /locus_tag="IWO31_002580"
     CDS             complement(1387..2322)
                     /locus_tag="IWO31_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0302952.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2624..4558
                     /locus_tag="IWO31_002581"
     CDS             2624..4558
                     /locus_tag="IWO31_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0302953.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5015..5431
                     /locus_tag="IWO31_002582"
     CDS             5015..5431
                     /locus_tag="IWO31_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0302954.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5445..6011
                     /locus_tag="IWO31_002583"
     CDS             5445..6011
                     /locus_tag="IWO31_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0302955.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6034..7020
                     /locus_tag="IWO31_002584"
     CDS             6034..7020
                     /locus_tag="IWO31_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0302956.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         2085 a         1381 c         1467 g         2228 t
ORIGIN      
        1 aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa aataaaaaag
       61 tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct accgcaaaag
      121 gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt tttagtgtca
      181 tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg gagtcctccg
      241 ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct tttgtcacga
      301 agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct tttttcacac
      361 ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct gttatgatcg
      421 tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac aaatgatggt
      481 attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact aatgttttgt
      541 tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct tggatgccga
      601 ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca ttaccgccca
      661 agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact tcgtccgtaa
      721 tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt agataaacag
      781 ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat gcctgatcga
      841 tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat tgtgtgattg
      901 caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag gcccgcttga
      961 ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc tcattcactt
     1021 catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct tttactcgat
     1081 cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata cgaacgtaag
     1141 tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta gctgctgaac
     1201 tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata aaagagatgc
     1261 ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct tcattttgaa
     1321 aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat tagtatgatt
     1381 atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt cgcctttttc
     1441 agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt gtgtagcacc
     1501 tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt tgacgaattc
     1561 gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt tcatattttc
     1621 attatattct gtatcatctt gttttgattt accgtctttg tcgatataat caaagtttga
     1681 ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc cgttagagta
     1741 gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt cgatttcgat
     1801 tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt tttggaagct
     1861 ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt gtgcttttaa
     1921 gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt ttgcttccat
     1981 gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga cgttatcata
     2041 ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac ggtaaccatt
     2101 tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc cagctacaac
     2161 ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag atgattcaga
     2221 agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta gagctgaaaa
     2281 tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac cccaccattc
     2341 catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt tttagcctat
     2401 aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct ttcagcaaat
     2461 ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt tagacaaaag
     2521 aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta aaagaattat
     2581 catatattca ttatatagat agtctataag gagcggatga tcaatggcaa agcaaaatat
     2641 tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg caaagaaact
     2701 gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata ctatgatgac
     2761 cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat acgatctgca
     2821 gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa tcggtatcga
     2881 taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc agctgatcct
     2941 tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac acggattcac
     3001 cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga cagtagcaaa
     3061 agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg ttgtctgtgg
     3121 ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa atcgcctagc
     3181 caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg caatgccgac
     3241 gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt tgcgtaagca
     3301 caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc atatcaaatt
     3361 gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg tcaaaggaac
     3421 ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga tcgccaatga
     3481 atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg atttagtctt
     3541 ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg aacaaacagg
     3601 acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc ataaatttaa
     3661 aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag ctaacctatt
     3721 tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg tgaatttgaa
     3781 atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc atgagttttt
     3841 ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg gcaatgtctt
     3901 atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag cttccaaaaa
     3961 aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag gatcatggtt
     4021 cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg ttgccagtgg
     4081 tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa gtgccggaga
     4141 agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag aaccaatggc
     4201 tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca tgccaaacaa
     4261 tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct tggcattagc
     4321 attgattgct ggattattca catggctatg cagtgcgaca acgattgtat tggtgatcac
     4381 gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg ttgctttcgc
     4441 attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc catggctaca
     4501 acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca agaaataaat
     4561 agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga caaaccgtca
     4621 cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc tccatgagca
     4681 agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt ccagaagtag
     4741 cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt cgtaaattcc
     4801 ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac ggtgttccac
     4861 ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg ttttctattc
     4921 ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt tatgctaaaa
     4981 ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat ttgctgaaaa
     5041 aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc tttctttttt
     5101 accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag aagcggtact
     5161 gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag agagcaacac
     5221 atatacttac aaagatgaac acggagatta taacttgatc gagatagatg gcgatcgagt
     5281 acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct gggcaacgaa
     5341 aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc gttcaacaga
     5401 tggaggtgac gaagacagtc tgatttatta gaccttcaca caccatgagt aaattacaaa
     5461 aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta ttggaaaata
     5521 tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc aatctaatca
     5581 cgatcgttgc catttttacc atgaaaaaac aagacagctt cttactactt tggctacgac
     5641 tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt atgagtggtg
     5701 ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa cgtgtcagta
     5761 tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt ttgatggctt
     5821 ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca gttttaggga
     5881 ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat gtgcagacac
     5941 ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc ccaatgttta
     6001 ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat gtgggaaaac
     6061 tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga aactgtccgc
     6121 ctgaaaaaca aacctgtcga acaagccatc aaagaaacga tccatgccgg cggaaagctg
     6181 ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga tcgagacaaa
     6241 gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat ccatgatgat
     6301 atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca gtttggtaat
     6361 gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact attgtctgat
     6421 tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa agtattgaat
     6481 ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa agaatatttg
     6541 gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt tggcgctttt
     6601 gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact ttctatcggg
     6661 atggcctttc agattatcga tgatatttta gattatacaa aagagagcca ggaaatcggc
     6721 aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat ctactctttg
     6781 caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat gacagaagaa
     6841 gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga agctaggaag
     6901 ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc tgccacctct
     6961 cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg caaagactga
     7021 atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt gttttttgac
     7081 aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc aacgtccgtt
     7141 agcgacgttg ctctttttta t
//
LOCUS       DACECS010000102         7153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.103, whole genome shotgun sequence.
ACCESSION   DACECS010000102 DACECS010000000
VERSION     DACECS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.103"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1368)
                     /locus_tag="IWO31_002585"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="IWO31_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ0302957.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="IWO31_002586"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="IWO31_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0302958.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="IWO31_002587"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="IWO31_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="IWO31_002588"
     CDS             complement(2652..2882)
                     /locus_tag="IWO31_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0302959.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="IWO31_002589"
     CDS             complement(3112..3930)
                     /locus_tag="IWO31_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0302960.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="IWO31_002590"
     CDS             4091..4780
                     /locus_tag="IWO31_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0302961.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="IWO31_002591"
     CDS             4794..6296
                     /locus_tag="IWO31_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0302962.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="IWO31_002592"
     CDS             6309..6785
                     /locus_tag="IWO31_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0302963.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="IWO31_002593"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="IWO31_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2316 a         1131 c         1295 g         2411 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DACECS010000103         7046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.104, whole genome shotgun sequence.
ACCESSION   DACECS010000103 DACECS010000000
VERSION     DACECS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.104"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IWO31_002594"
     CDS             94..1248
                     /locus_tag="IWO31_002594"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0302964.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IWO31_002595"
     CDS             complement(1270..2022)
                     /locus_tag="IWO31_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0302965.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IWO31_002596"
     CDS             complement(2386..5052)
                     /locus_tag="IWO31_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0302966.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IWO31_002597"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IWO31_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0302967.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2072 a         1455 c         1277 g         2242 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttc
//
LOCUS       DACECS010000104         6652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.105, whole genome shotgun sequence.
ACCESSION   DACECS010000104 DACECS010000000
VERSION     DACECS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.105"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(7..1164)
                     /locus_tag="IWO31_002598"
     CDS             complement(7..1164)
                     /locus_tag="IWO31_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302968.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="IWO31_002599"
     CDS             complement(1167..1523)
                     /locus_tag="IWO31_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302969.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2741)
                     /gene="sgrA"
                     /locus_tag="IWO31_002600"
     CDS             complement(1821..2741)
                     /gene="sgrA"
                     /locus_tag="IWO31_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0302970.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2937..3776)
                     /locus_tag="IWO31_002601"
     CDS             complement(2937..3776)
                     /locus_tag="IWO31_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0302971.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3961..4848
                     /locus_tag="IWO31_002602"
     CDS             3961..4848
                     /locus_tag="IWO31_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0302972.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5432..6127)
                     /locus_tag="IWO31_002603"
     CDS             complement(5432..6127)
                     /locus_tag="IWO31_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0302973.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6111..6509)
                     /locus_tag="IWO31_002604"
     CDS             complement(6111..6509)
                     /locus_tag="IWO31_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0302974.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2103 a         1300 c          978 g         2271 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca
     2221 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     2281 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     2341 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     2401 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     2461 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     2521 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     2581 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     2641 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     2701 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     2761 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     2821 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     2881 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     2941 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     3001 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     3061 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     3121 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     3181 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     3241 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     3301 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     3361 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     3421 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     3481 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     3541 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     3601 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     3661 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     3721 acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     3781 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     3841 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     3901 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     3961 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     4021 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     4081 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     4141 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     4201 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     4261 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     4321 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     4381 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     4441 aaagcaaaaa aagaagcaaa tgaaggggaa aatttctcca agcttgttca agcttatgga
     4501 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     4561 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     4621 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     4681 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
     4741 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
     4801 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
     4861 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
     4921 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
     4981 ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga
     5041 atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc
     5101 ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa
     5161 aatatccctt ctggacttgt tcctcttatt ctgttatact agagaaacac gaaagatatt
     5221 tttatgcgac acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga
     5281 gttacaagga tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt
     5341 cacccaaact ttaaattaat gaatattgaa tcgtcagata gcctcctcga gttttcgaga
     5401 aggctatcgt ttattttatg gagaaattga ttcagaagta atcagcgacc ttttttagtt
     5461 caccaatcgt ttctgcttgg aaagtttgca cccaatcttc ttggaagccc ctcactgctt
     5521 tatcaatcga gggcatctct aatacttgat ctagtagctc tttacctaac gttatcgcat
     5581 gagctccaga tcgaatggct gtagtgactt gttcacaatt tttgaaacta gctgctaata
     5641 tttttgttgg tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc actttcatag
     5701 catcaatgcc ttgattttgc atcctgttat aataaggtgc aatataatct gctccattat
     5761 taatagctaa atacccttgt aattctgtgt aaatagctgt accggtaatc aaataacctt
     5821 cttttttcaa ctttttgatc gcttgaaggc ctgcttgatt caccggcacc ttgataaacg
     5881 tatcttttcc cagttcttct atgataactc gagcatcctc tatcatttct tcttccgttc
     5941 ttcctattgt ctgaatatgg ataggaatct gtccgattat ttttttgatt tctttcatat
     6001 gcgagaagaa gtttgacgct ccttcttttt tcagaattgt aggatttgtt gttacgcctg
     6061 ttaaattcaa aaactgttga caagaataaa tactttttgt atttgctgta tcaagcatga
     6121 tttccatata gctttccccc attctgatcc tcttgatcga ttttaatgat tgtccccttt
     6181 cttaaatgcg gcatttcctt atcttctaca tacattgttc ctggaagttc cggctcatga
     6241 gatccatcaa attttatcgt gatatgtcct aattcattca aattagtttg gacaacatct
     6301 cctactgctg tgattacata catttgttta tcaaaacaga gcttcatatc acaagttatt
     6361 tcttttctta cctgattcaa ttcaatctta taacaataat ctgttaatgt ttccggtgct
     6421 tcatctccaa ataaaatcat tattttttct ttcctgaata cttctgcttc tataccaaca
     6481 tctgttacaa ccgtttcaaa gatcgtcatc tctgttcctc ctcgtaaaat atcatttgtc
     6541 taatagtttt tattcaaaat gtttctagat tataaaattt attgaataac acattcctaa
     6601 cggaatttgt ttcgatattg tgtgatttct cagagtacaa tggatgtatg ga
//
LOCUS       DACECS010000105         6503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.106, whole genome shotgun sequence.
ACCESSION   DACECS010000105 DACECS010000000
VERSION     DACECS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.106"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            60..512
                     /locus_tag="IWO31_002605"
     CDS             60..512
                     /locus_tag="IWO31_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302975.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            526..1215
                     /locus_tag="IWO31_002606"
     CDS             526..1215
                     /locus_tag="IWO31_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302976.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1340..3691
                     /locus_tag="IWO31_002607"
     CDS             1340..3691
                     /locus_tag="IWO31_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0302977.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3771..4166
                     /locus_tag="IWO31_002608"
     CDS             3771..4166
                     /locus_tag="IWO31_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302978.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4491..5108
                     /locus_tag="IWO31_002609"
     CDS             4491..5108
                     /locus_tag="IWO31_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0302979.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5149..5448
                     /locus_tag="IWO31_002610"
     CDS             5149..5448
                     /locus_tag="IWO31_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302980.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5482..5649
                     /locus_tag="IWO31_002611"
     CDS             5482..5649
                     /locus_tag="IWO31_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0302981.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5694..6299)
                     /locus_tag="IWO31_002612"
     CDS             complement(5694..6299)
                     /locus_tag="IWO31_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0302982.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2247 a          898 c         1279 g         2079 t
ORIGIN      
        1 accaattatc ttatcgtaga tatatattat aaatttaata agaaagcgag gtgagaatcg
       61 tgaaaaaaac gatactaagt attttttcag tatttacaat attaattggg ggctattttc
      121 ttttgaattt agattctgtt aattcaaacg ctcaaataaa agatcaaaga gtagctgaac
      181 ataataataa aaaaatgagt ttgaccaaaa atatctattc tatgtcagat gaagaattgt
      241 ataaagaatt tggtgattta tccggggaac agttagaaga aagtattcca ggaattagtg
      301 actcagataa aattgttgtc ttgtctgctg gtgggcaatt aatgggagat ggcaaatccg
      361 aattagttag ttataaacaa aatcacagac agtttattga tgttaatact gattccaacg
      421 ctatttcgat taaggatcta aaagagcttt taaatagtaa aagaaatgaa ttaaagcaga
      481 taaaagaaac taattcagtg gaattagagt aaaggaagat tcattatgaa aaagattttt
      541 ggtgtttctg ttgggctact tatgatgtta acatttggaa atgcagtata tgctgctgag
      601 aaagaagtta tttcagatcc agagcagtac gcctcacgat actttgctga ttttgaagaa
      661 gaagataaag tacttgcatt gccagaaggt ggttttttaa aaggggaagc taaaattgta
      721 tctatggata atccagatat aattcaaggt tcatataatt cagaaactga tcctgcttca
      781 gtttcagtga cagaagcaaa aaaaattgtt gctgaaaaat tcaaaaatga tattgaaact
      841 actccaatta tgcctcgagg agcatttcca cccgatgttg gtactagcgg acaccttctt
      901 gtactagcag gaggaggaag ctataaatca cagccattta gtggtgcggg atggcgtttt
      961 ggaggtatgg gtgtaaaacc agctgatggt acaggcaact ttttacgttg gaaatcctac
     1021 attgacgatg gaagagtagg aaatgtaaat caagccaacg caactaaatc tggttctatt
     1081 caaggaatgg cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa cgggcctggt
     1141 tggacaatga atataggtat ttattacact tataatcctg ttgcaggtac gtattatgtt
     1201 gttcaaaata tttaataaaa cagcacaaag agatttcttt gtgctgtttt attattttta
     1261 gttgttgaca aacactaaaa tataaaatat aatcaacaca taaacgaacg tatgttcggc
     1321 aagaaagcag gggattttta tggatgagat ggttttaagt actcagaaat ggctaaacaa
     1381 gaaatacagt aatgttacag ggtttgataa ggttccagaa aatgggcgaa cagggtggcc
     1441 tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta atctagtagc
     1501 taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta aatggggcaa
     1561 aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta aggggattaa
     1621 tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa aagagttgca
     1681 gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga aatgggccaa
     1741 agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta aaattagaaa
     1801 aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc cttgtgatgg
     1861 tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg aactaggata
     1921 ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa cttatcctgt
     1981 gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat atgttaatgg
     2041 ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt tagaagtact
     2101 ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg ttgttgtaat
     2161 aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg cagatacttc
     2221 tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa aaatagttgg
     2281 tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt atctaactac
     2341 agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct atcaagatgg
     2401 aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta agaaggctat
     2461 agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg ctgttgatct
     2521 agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg gggtatctgc
     2581 tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg tttgttctca
     2641 aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga ctggttatag
     2701 tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg ttgaatttac
     2761 cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg gtagagatag
     2821 cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca aatacaatca
     2881 gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg gtcttgataa
     2941 tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt tgtcatggga
     3001 catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat ataggaataa
     3061 attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg gttcaataat
     3121 aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg acacaatcaa
     3181 tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct ggggtgaagt
     3241 taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta cggctgcagg
     3301 tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg tttatacctt
     3361 agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact attgtgataa
     3421 gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt cagataaaat
     3481 cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct taggtgtagg
     3541 gataatttca tttttcattg ataaaatagt taataattca cttgggacag attttactta
     3601 tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa aaattattca
     3661 atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa cagtgtcctt
     3721 ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac atgatgatta
     3781 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
     3841 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
     3901 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
     3961 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
     4021 caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag
     4081 aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag
     4141 aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc
     4201 aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact
     4261 agtttattta atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat
     4321 ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga
     4381 ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt
     4441 aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat
     4501 ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga
     4561 aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta
     4621 aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac
     4681 aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa
     4741 ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt
     4801 acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct
     4861 atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg
     4921 cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg
     4981 cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg
     5041 gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact
     5101 tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg
     5161 aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt
     5221 ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg
     5281 gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa
     5341 accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt
     5401 tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa
     5461 tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata
     5521 actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa
     5581 agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat
     5641 ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa
     5701 tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc
     5761 tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt
     5821 tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt
     5881 tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca
     5941 atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca
     6001 gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata
     6061 aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg
     6121 atgacgcaat caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta
     6181 tctttaccac tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc
     6241 aattgtcgat ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata
     6301 aaaaactcct ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc
     6361 tatcatagca tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt
     6421 taaatggatg aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt
     6481 acagaacaat taaaccaatt ttt
//
LOCUS       DACECS010000106         6401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.107, whole genome shotgun sequence.
ACCESSION   DACECS010000106 DACECS010000000
VERSION     DACECS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.107"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWO31_002613"
     CDS             <1..177
                     /locus_tag="IWO31_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302983.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWO31_002614"
     CDS             190..690
                     /locus_tag="IWO31_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0302984.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWO31_002615"
     CDS             787..2634
                     /locus_tag="IWO31_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0302985.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWO31_002616"
     CDS             2637..3533
                     /locus_tag="IWO31_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0302986.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWO31_002617"
     CDS             3583..3972
                     /locus_tag="IWO31_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0302987.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..6304
                     /locus_tag="IWO31_002618"
     CDS             3959..6304
                     /locus_tag="IWO31_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0302988.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
BASE COUNT         2161 a          950 c         1370 g         1920 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacggga ttacgtccat
     6301 ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga gtacaatgtt
     6361 ttttgcagaa aacatactcg aataggactg atatcaatga c
//
LOCUS       DACECS010000107         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.108, whole genome shotgun sequence.
ACCESSION   DACECS010000107 DACECS010000000
VERSION     DACECS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.108"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWO31_002619"
     CDS             334..714
                     /locus_tag="IWO31_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0302989.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWO31_002620"
     CDS             696..1610
                     /locus_tag="IWO31_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0302990.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWO31_002621"
     CDS             1855..2544
                     /locus_tag="IWO31_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302991.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWO31_002622"
     CDS             complement(2665..2751)
                     /locus_tag="IWO31_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0302992.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWO31_002623"
     CDS             3427..4377
                     /locus_tag="IWO31_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0302993.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWO31_002624"
     CDS             4398..4931
                     /locus_tag="IWO31_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0302994.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWO31_002625"
     CDS             5567..6100
                     /locus_tag="IWO31_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302995.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACECS010000108         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.109, whole genome shotgun sequence.
ACCESSION   DACECS010000108 DACECS010000000
VERSION     DACECS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.109"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWO31_002626"
     CDS             725..811
                     /locus_tag="IWO31_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0302996.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWO31_002627"
     CDS             complement(932..1621)
                     /locus_tag="IWO31_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302997.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWO31_002628"
     CDS             complement(1628..1906)
                     /locus_tag="IWO31_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0302998.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWO31_002629"
     CDS             complement(1866..2780)
                     /locus_tag="IWO31_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0302999.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWO31_002630"
     CDS             complement(2762..3142)
                     /locus_tag="IWO31_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0303000.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWO31_002631"
     CDS             complement(3592..3879)
                     /locus_tag="IWO31_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0303001.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWO31_002632"
     CDS             complement(3899..4123)
                     /locus_tag="IWO31_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0303002.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWO31_002633"
     CDS             complement(4763..5296)
                     /locus_tag="IWO31_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0303003.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWO31_002634"
     CDS             complement(5317..6159)
                     /locus_tag="IWO31_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0303004.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagcgc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtatatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACECS010000109         6146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.110, whole genome shotgun sequence.
ACCESSION   DACECS010000109 DACECS010000000
VERSION     DACECS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.110"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IWO31_002635"
     CDS             101..2542
                     /locus_tag="IWO31_002635"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0303005.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IWO31_002636"
     CDS             complement(2872..3576)
                     /locus_tag="IWO31_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303006.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="IWO31_002637"
     CDS             complement(3763..4008)
                     /locus_tag="IWO31_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303007.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="IWO31_002638"
     CDS             complement(4025..4876)
                     /locus_tag="IWO31_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0303008.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="IWO31_002639"
     CDS             5042..6046
                     /locus_tag="IWO31_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0303009.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1811 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtg
//
LOCUS       DACECS010000110         6133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.111, whole genome shotgun sequence.
ACCESSION   DACECS010000110 DACECS010000000
VERSION     DACECS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.111"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWO31_002640"
     CDS             complement(207..1169)
                     /locus_tag="IWO31_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0303010.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWO31_002641"
     CDS             complement(1171..2610)
                     /locus_tag="IWO31_002641"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0303011.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWO31_002642"
     CDS             2795..4741
                     /locus_tag="IWO31_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0303012.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IWO31_002643"
     CDS             5005..5877
                     /locus_tag="IWO31_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0303013.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DACECS010000111         6097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.112, whole genome shotgun sequence.
ACCESSION   DACECS010000111 DACECS010000000
VERSION     DACECS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.112"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            54..947
                     /locus_tag="IWO31_002644"
     CDS             54..947
                     /locus_tag="IWO31_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0303014.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1081..1206
                     /locus_tag="IWO31_002645"
     CDS             1081..1206
                     /locus_tag="IWO31_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0303015.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1340..1972)
                     /locus_tag="IWO31_002646"
     CDS             complement(1340..1972)
                     /locus_tag="IWO31_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303016.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2120..2662)
                     /locus_tag="IWO31_002647"
     CDS             complement(2120..2662)
                     /locus_tag="IWO31_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0303017.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2797..3186
                     /locus_tag="IWO31_002648"
     CDS             2797..3186
                     /locus_tag="IWO31_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303018.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3190..3954
                     /locus_tag="IWO31_002649"
     CDS             3190..3954
                     /locus_tag="IWO31_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0303019.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            4083..5030
                     /locus_tag="IWO31_002650"
     CDS             4083..5030
                     /locus_tag="IWO31_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0303020.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            5160..5900
                     /locus_tag="IWO31_002651"
     CDS             5160..5900
                     /locus_tag="IWO31_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303021.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         2144 a          987 c         1192 g         1774 t
ORIGIN      
        1 ttttacgtaa aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact
       61 attattcaaa aaataaggaa aacttttatc aaaaactgac cgatgatccg cttttttctt
      121 cattgaccga ttatctatat gaacatcgag aacaagaaac gattctaaga gaattgaaaa
      181 aagaattttc gcaaaataaa ttttcccatt ttcttgattt attgatcgat gcaggattaa
      241 tcaagaggga agaacgaaga tatcatttga actttccaat atttgatcct aaagattact
      301 tgcaacaagc cacttcggct gctgaaacca tagctgaaca attgaagaga ctctctgttg
      361 atgaacaaaa attagcgatg ggagaagtta tttgggcgta ttgtttcgaa gatgaacgga
      421 aagaagcgta tttttatgga gtaagaaaca gccgagagac agaattgctg aggacaactg
      481 caggaaatga gaagtaccgt tttatcacac tttcctctat agaacacttc ccgcttactt
      541 tggctaatta cttttttgta cagaagaatc agcttcctgt aacgaaggca ttcaaagaat
      601 tagccgaact tattggagac gtaaatgaat cgtacttttt tgatcaaata gaagttatcg
      661 ttgacaggat acgtaagaat aaatataaaa atcgccggcc tagtattttc catcagtctc
      721 tacttgtgac gaatactata aaggaagaag aatcattcac attagaatta cctattgtcg
      781 aaaaaaacaa ttttgagatt gaattaccaa cattggatcc ttcattgaca atggaggaaa
      841 tggcctttct taaacgacaa atcttttctg agctgagcaa gaaatttata ccgcatgcat
      901 tttcttatat caaagaatat ggaacagttt tagtgagtaa aacctaagag agcagtaagc
      961 aaaaattata ctgactcttg caggaggagc ctttctcttc ctttatggtt aaaagaatta
     1021 tttgagtatt ttgcctatct accacctatt agattcacta catggtctgg aaacaaagga
     1081 atgcaaaaaa atggtgagaa atgtggaatg acaaaggaag tagtgataag aaaagttaga
     1141 tttttaaata atcaatatta tgattcagtc aagtatggca ttttatggga agaactagct
     1201 gattagatta ggaagtccat ttgataatcc agaatcacta agaaacgaat ggcgagtcat
     1261 taataattta tccaatcttg ggttattctt ttagattgaa gtgtttaaaa ctaaaaaagc
     1321 atttatgaag tcgtaatagt caggcttcaa aatgcttttt attttgtttg ttttttcttt
     1381 gccacataaa tacacctaga ctgacgatga agagcatatc caacagattt cctgcgactt
     1441 ggatcgtcga agggttggtc agtggcatta aagcaaggac aaaaaaggaa aacaagctgt
     1501 aaataaaaca ggtgacagga actaatcgaa agaagagcgg acatttcaat tggctaaaaa
     1561 tgagtgaaaa aataaaataa gtaaaaatgg ataaaatcac gactaaacca ttcatgataa
     1621 agaagaaaag cgtattgtga ttaaacgaat aaaaaagaat tgctaaaaaa gaaaacgttt
     1681 taaatgcaat caatacgctt gaagcttcgt ttaacgtacc aggatgtcga tgaaaaaaat
     1741 aggcaccgaa ataaacagaa aacagtaagc cgcaaagaga agacaaaatc aaaaaaaacg
     1801 gattttcttc ggcttctgtg atatcagaat gatccaaaag caaccagcct aatgcactag
     1861 aaggcgcatg tgaggtctta aggtatgaat aacatagaaa aaataagatc gtcaaagttg
     1921 caaaaaaaga gcctaaatgt aaaaaattag tggttctacg ttgaatttcc ataatatctc
     1981 caaattaaat tgataagtat ataaagtata ataacaaatt gatgagtata tgtcgatgtt
     2041 ttttaatcaa aataataaat aaaaactgtc taacatttaa aatttaattt agatattaga
     2101 cagtttttaa gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac
     2161 cacctgaaat atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga
     2221 tcaggcgata cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct
     2281 caccttccag ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac
     2341 ccggcaaact tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc
     2401 ctaatgccaa tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg
     2461 catagacaaa taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga
     2521 tataaatcgt gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg
     2581 tcaacatgaa acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc
     2641 ccaacatttt tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa
     2701 aaaaaatcgt aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat
     2761 tataatagaa ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt
     2821 tgtgccagcc acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa
     2881 cagccaattg aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta
     2941 agcgtttttt tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt
     3001 ttgaattttt ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct
     3061 ctgtgcttat ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg
     3121 ttccagacaa ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt
     3181 gagtaacaat tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa
     3241 gttgttctga aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg
     3301 cagacaagta aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt
     3361 gtgatcctgc cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca
     3421 tcctatacat tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag
     3481 catttagaaa aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg
     3541 ggaatgacaa catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg
     3601 atgggttccc ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat
     3661 aattatgtcg taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg
     3721 tcattgcagc cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac
     3781 gaaaaaatcc cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac
     3841 ggaaaagaaa ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aataccacta
     3901 atggaagaag cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa
     3961 cggaaaatca ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact
     4021 ctgttaggaa aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga
     4081 gcatgcccac tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc
     4141 gagtcatcaa tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa
     4201 tggctgaact ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc
     4261 agatcatcaa attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt
     4321 tgctgatggg aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa
     4381 atgatgcctt cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag
     4441 acttcgattg gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag
     4501 gatatgatta tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct
     4561 tagaaagaga ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa
     4621 tttcaaggga aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct
     4681 tgcggtttcc aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt
     4741 ttacagataa aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag
     4801 ggattataaa tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg
     4861 tatttttaga ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta
     4921 tgggtgaagt atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag
     4981 gttatcgtcg tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac
     5041 tatatatata tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg
     5101 aaaacaatga atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa
     5161 tgaaagaaat cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa
     5221 aattaaccaa cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac
     5281 agaatcagtc cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga
     5341 aaatccgtcg caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt
     5401 tggaaaagca taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat
     5461 cgacaggacg aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa
     5521 ggacagagtc caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt
     5581 cttattttgt tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa
     5641 tttctgagga tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg
     5701 atacagaacc gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca
     5761 atcgaacaaa aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc
     5821 atggtatctt agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag
     5881 aaaatagcta ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa
     5941 acgctgcggc gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt
     6001 tttttgagtt taagcgaatt ttttctccca ataagtaaga aaataatggc aaaataaaaa
     6061 aacaaactat tttcgcagat cgtttgcaaa aatagtt
//
LOCUS       DACECS010000112         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.113, whole genome shotgun sequence.
ACCESSION   DACECS010000112 DACECS010000000
VERSION     DACECS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.113"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWO31_002652"
     CDS             <1..395
                     /locus_tag="IWO31_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303022.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWO31_002653"
     CDS             398..1228
                     /locus_tag="IWO31_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0303023.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWO31_002654"
     CDS             1244..2095
                     /locus_tag="IWO31_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0303024.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWO31_002655"
     CDS             2092..2451
                     /locus_tag="IWO31_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0303025.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWO31_002656"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWO31_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWO31_002657"
     CDS             2624..2929
                     /locus_tag="IWO31_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303026.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWO31_002658"
     CDS             2929..3240
                     /locus_tag="IWO31_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303027.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWO31_002659"
     CDS             3407..3823
                     /locus_tag="IWO31_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0303028.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWO31_002660"
     CDS             3823..4491
                     /locus_tag="IWO31_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0303029.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWO31_002661"
     CDS             4488..4865
                     /locus_tag="IWO31_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303030.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWO31_002662"
     CDS             4862..5161
                     /locus_tag="IWO31_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303031.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWO31_002663"
     CDS             5403..5819
                     /locus_tag="IWO31_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0303032.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACECS010000113         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.114, whole genome shotgun sequence.
ACCESSION   DACECS010000113 DACECS010000000
VERSION     DACECS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.114"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..1170)
                     /locus_tag="IWO31_002664"
     CDS             complement(76..1170)
                     /locus_tag="IWO31_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0303033.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IWO31_002665"
     CDS             1359..2120
                     /locus_tag="IWO31_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0303034.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWO31_002666"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWO31_002666"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0303035.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IWO31_002667"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IWO31_002667"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0303036.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IWO31_002668"
     CDS             complement(3760..4773)
                     /locus_tag="IWO31_002668"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0303037.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IWO31_002669"
     CDS             4938..5858
                     /locus_tag="IWO31_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0303038.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACECS010000114         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.115, whole genome shotgun sequence.
ACCESSION   DACECS010000114 DACECS010000000
VERSION     DACECS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.115"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWO31_002670"
     CDS             450..1604
                     /locus_tag="IWO31_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0303039.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWO31_002671"
     CDS             1598..2020
                     /locus_tag="IWO31_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303040.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWO31_002672"
     CDS             3143..4075
                     /locus_tag="IWO31_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0303041.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWO31_002673"
     CDS             4092..4607
                     /locus_tag="IWO31_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0303042.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWO31_002674"
     CDS             4903..5184
                     /locus_tag="IWO31_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303043.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWO31_002675"
     CDS             5186..5602
                     /locus_tag="IWO31_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303044.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          962 c          930 g         1689 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtacacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACECS010000115         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.116, whole genome shotgun sequence.
ACCESSION   DACECS010000115 DACECS010000000
VERSION     DACECS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.116"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO31_002676"
     CDS             complement(99..1229)
                     /locus_tag="IWO31_002676"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0303045.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWO31_002677"
     CDS             complement(1877..3079)
                     /locus_tag="IWO31_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0303046.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWO31_002678"
     CDS             complement(3139..3342)
                     /locus_tag="IWO31_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0303047.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWO31_002679"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWO31_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWO31_002680"
     CDS             4879..5691
                     /locus_tag="IWO31_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0303048.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACECS010000116         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.118, whole genome shotgun sequence.
ACCESSION   DACECS010000116 DACECS010000000
VERSION     DACECS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.118"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO31_002681"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO31_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO31_002682"
     CDS             complement(546..740)
                     /locus_tag="IWO31_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303049.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IWO31_002683"
     CDS             2092..3132
                     /locus_tag="IWO31_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0303050.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IWO31_002684"
     CDS             3956..4288
                     /locus_tag="IWO31_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0303051.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IWO31_002685"
     CDS             4907..5248
                     /locus_tag="IWO31_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0303052.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DACECS010000117         4873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.121, whole genome shotgun sequence.
ACCESSION   DACECS010000117 DACECS010000000
VERSION     DACECS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.121"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            184..681
                     /locus_tag="IWO31_002686"
                     /pseudo
     CDS             184..681
                     /locus_tag="IWO31_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405295.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(766..1362)
                     /locus_tag="IWO31_002687"
     CDS             complement(766..1362)
                     /locus_tag="IWO31_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0303053.1"
                     /translation="MDGFERRTEEKKKKVLRAAFDLMNTDTGIENLTIDAVAKKANVG
                     KTSIFKYFGSKEKLIHEVFIRSMDEMGEKAKKIMEKNASFEETLIAMSQNKIDYLEKI
                     NKQFYLDLMSYYTKKEDSGFTRMMEAYTKQSFGIMLDLFHRGRKEGKVDLKYSDEFLM
                     LYFQAMIEGISNPQIYEKILPYTAEWTELLVKGIAPRQ"
     gene            1673..2554
                     /locus_tag="IWO31_002688"
     CDS             1673..2554
                     /locus_tag="IWO31_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0303054.1"
                     /translation="MTEIVKVQGLQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKS
                     TTIRTLLGIINRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFI
                     KLHGGGSKAKRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSG
                     LDPLMEAVFQEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELR
                     HLTRSTVTLVTKGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVIKI
                     ESVPPTLEDLFMRHYEG"
     gene            2578..4191
                     /locus_tag="IWO31_002689"
     CDS             2578..4191
                     /locus_tag="IWO31_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0303055.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(4263..4775)
                     /locus_tag="IWO31_002690"
                     /pseudo
     CDS             complement(4263..4775)
                     /locus_tag="IWO31_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1542 a          834 c          973 g         1524 t
ORIGIN      
        1 tggaaagctt gcagccacct acaccaccaa tacccaaaac acctaaactt ccaggtattt
       61 aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
      121 aacatttcct caaacgccta acaaaaatgg ttgtcaaaaa gccaaaaatc aaaaagaaaa
      181 ggaatgataa aaatgaaata cattaaaaac gttgaaacat tggaagaatt gaaaaaagcc
      241 tacaaaaagt tagctttgaa gttacacccc gattgtggag ggaatgaaga agaaatgaaa
      301 actttgaaca atgaatatga cgaactgttt agcaaactta aaaacactca taagaataaa
      361 gacggtgaaa cttacaccaa agaaacaaca gaaacacccg aacagtttaa ggatattatt
      421 aataaattgt tcaatctaaa aatggacggc gtagcaatcg aagtagtagg aacatttata
      481 tgactaacag gtaatacaaa accatataaa gaagatataa aagccctaga attccgatat
      541 tcacctaaaa aatatgcttg gtataaagcc cctaaagatt acaaaaaaag aagccgacta
      601 cgatcaatct gtgagaagcg gctgtctctg agacaattgc atcacatttt agttgtgaat
      661 atgtatctca aatacaccta gcagatgaat cgattctgat ttgtatgcat acattttatt
      721 tttttgtgga ttcggcgagt atttcttctt ttgttgttcg taaaactatt gtctcggagc
      781 gattcctttt accaacagct cagtccattc cgctgtataa ggcaggattt tctcataaat
      841 ctgcggatta gagatgcctt cgatcatggc ttgaaaatac aacatcaaaa attcatctga
      901 atattttaaa tcgacttttc cttctttacg cccgcgatgg aataaatcca acataatgcc
      961 aaaactttgc tttgtataag cttccatcat tcgggtaaag ccgctgtctt cttttttagt
     1021 gtaatagctc attaaatcta ggtaaaattg tttattgatt ttttcgaggt aatcgatttt
     1081 attttgactc atggcgatca aagtttcttc aaatgatgca tttttctcca tgattttttt
     1141 agctttttcc cccatttcat ccattgaccg gataaagact tcatgaatga gcttttcttt
     1201 gctgccaaaa tatttaaaaa tagaagtttt tcctacattg gcctttttgg ctacggcatc
     1261 gatcgttaaa ttctcgattc ccgtatctgt attcatcaga tcaaaagcag ctcgtaaaac
     1321 ttttttcttt ttttcttctg ttcttcgttc aaaaccatcc attaaaaata cctactttct
     1381 ataaaaaaga actttttgtt tatcatagtt caaaaatagc ttcgtatata ttatatagga
     1441 aataatacga actaacaatg gaatcatagg tttaaattta aaattatttt tattgactgt
     1501 taaaataaaa aagaatatta ttggagctgt aaaagaactt tggtttgaaa caaagtataa
     1561 aggggtattt tatgaacgat acgtattgta ttggttcata aaaaaggtgc gttttgttca
     1621 gactggaaag ctcgttataa aatcatgtca aaagaaaagg gagttatgta ccatgacaga
     1681 aattgtaaaa gtacaaggct tgcaaaaaaa atttggtaaa ttccaggcgt tgaaagatgt
     1741 ctcattcaca gtaaacgccg gtgaagttgt tggttttatc ggaccaaatg gagcaggaaa
     1801 gtcaacgacg attcgtacac tgctaggaat catcaaccga gacgaaggag atgtccaaat
     1861 attcggaaaa gatgtttgga aagatagtct agaaatccat aaacgaattt cgtatgttcc
     1921 tggggatgtt gctctttggg gcagcctgac tggtggagag atcattgatc tatttatcaa
     1981 acttcatggc ggcgggagca aagcaaagcg tgattattta atcaaacgat ttgaacttga
     2041 tccaaagaaa aaagccaaag gttactctaa aggaaatcgt caaaaagtcg gtttgattgc
     2101 tgcactttca gttgaatctg atctgtatat tttagatgaa ccgacttcag gactagatcc
     2161 attgatggaa gcagtattcc aagaagaagt agaaaaaatc aaaaatgatg gcaaagcgat
     2221 tctattatct tcacatattt taagtgaagt tgaacgatta gcagataaag tagcaatcat
     2281 tcgacgtgga gaagtagttg aaacaggtac attagatgaa ttgcgtcatt tgactcgctc
     2341 aacagttaca ttggtgacaa aaggcgatat tgagaaactt gcgacgctct ctggcgtgca
     2401 tgattttgtt caaaaagacg gcaaagcaac tttttctgct gacaatgaag cgatgaatac
     2461 gattctgacc gaggcaacca aattaggtgt gataaaaatc gaatctgtac cgccaacgct
     2521 cgaagattta ttcatgcgtc actacgaagg ctgattgtcg gaacggagga aaagaaaatg
     2581 aatgaaaaat ttgcgcgttg gaacgtattg ttcattcaat acgtgaaacg cgattggaaa
     2641 aaaataattg tttgggtttt aggtttgggt ttgttctcag gagcatttgt accagcattt
     2701 gaagagattg ctaaaggaca aggtctttta gggatgtttg aaacgatgca aaatccagcg
     2761 atgatctcga tggttggacc tacaccaatc aaaataggta cggattatac tttaggagcg
     2821 atgtatgctc aagagatgtt gctgttttgc ggattgttcg caatgattat ctcagcactt
     2881 catgtggtga gccacacgcg aaaagaagaa gaattaggtt tgactgaatt ggttcgctca
     2941 tttcgagtag gacgacaagc caattcatta gctgttatca gtgagatgct gttgatcaat
     3001 cttttattag gtcttttaat cggcggactc atgatgagtt ttggtgtaaa aacgattgat
     3061 gccgaaggag ctttcttgtt cggaggatca attgcattgg cgggaattat cggtggtgta
     3121 ttggcacttg tgatgtcgca gattatggcg acttctactg gagcaaccgg ctcgacatta
     3181 agtcttatag gacttttgta tatcgtgcgc gctggaacag atgtgtctaa tcttgattta
     3241 tcaatgttca atccaatggg atggatttac ttgacctatc ctttcacaga aaataactgg
     3301 ctaccattat tatttgcttt gatttttagt cttgttttta ccgtacttgc gtttgtgttg
     3361 gaagaacatc gcgacatggg cgcaggttat cttcctgaac gagaaggacg tgcgacggcg
     3421 aagaaatcac tactttctgt acctggtttg tttttcaaga ttaataaagg agtaatgatt
     3481 ggttggctga tcgcatttgt cgttatggga gctgcgtatg gctccattta tggagacatg
     3541 caagtctttc ttggcggaaa tgaactgatg aaacaaatgt tcactcaatc tggcgtttcc
     3601 attgaagaat cctttacggc aacgatcatg atggtaatga ttggattagt cacaatcttg
     3661 ccaatcgcgg tggtcaataa attatttgca gaagaaacaa gactgcatct gagtcaactg
     3721 tatgtaacga agattacgcg aggccaatta tattggacaa cgatattttt agctattttt
     3781 gctggagtcg taggtattgg cttagcatca gcgggattag gtggaacggc gatttctgcg
     3841 atgaaaaatg aatcgactat ggatctgacc gatttcttag ctgctggata caattttctc
     3901 ccttccatct tattttatat tggtttggct gctttagcgt taggctggtt gccaaaattt
     3961 ggaaaagtaa tctatgctta tctaggctat tcctttgctt tgaattattt cggcggaatc
     4021 ttagatttgc cggattggtt ctcaaaaact gcaattcaaa gttggattcc acgcttaccg
     4081 atggaaaaat ttgatggaac gatttttgca gtaattactg ttatcagtat cgtcttctta
     4141 tttgtcggct atttaggata caaacgccgt gatatggtag aaggcgctta aaaattgaat
     4201 ttctaaaaaa ccgttcatat ttacgaaagt cgatttgagc ggttttttta tggcttcaat
     4261 tgtcaaatta agtgcaacga ttcattaaaa aatacttcat aaggggtttt ccaatttaga
     4321 catttccgtg gtcgcgtgtt taatttattc gcccattctt gaatttgact atcgaaaata
     4381 cctgaaatat cttcaccttt gggtgaatat tctcgtaata aaccattagt attttcgttt
     4441 gtaccacgtt gccatggagc atgtggatct ggaaaataca ctttagtgcc atttaattct
     4501 agcgttattc gactatgttg ggagaattct tttcctctat cgggtgtaac ggttttacat
     4561 ttatcttctc ctacatttct tagtagattg atcatacaat caaccacatt tttagagatt
     4621 ttcttttcaa ccttaccaag tagggagaaa cgagatctac gatcgactag agttacaata
     4681 cgagccctac ctgttttacc ggctacagta tcagcttccc aattaccaat ctcaaggcgc
     4741 tcattggcag cagatggcct ttcatggatc ttattgtatg cagatgatcc aatgacagct
     4801 agggcaccac caaaacaagc gcatgatatt gttgtcaaaa acttaccgac caatttagaa
     4861 acccttcata aaa
//
LOCUS       DACECS010000118         4798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.122, whole genome shotgun sequence.
ACCESSION   DACECS010000118 DACECS010000000
VERSION     DACECS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.122"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWO31_002691"
     CDS             complement(<1..1097)
                     /locus_tag="IWO31_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0303056.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IWO31_002692"
     CDS             complement(1227..2603)
                     /locus_tag="IWO31_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0303057.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="IWO31_002693"
     CDS             complement(2603..3259)
                     /locus_tag="IWO31_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0303058.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="IWO31_002694"
     CDS             complement(3269..4546)
                     /locus_tag="IWO31_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0303059.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..4720)
                     /locus_tag="IWO31_002695"
                     /pseudo
     CDS             complement(4565..4720)
                     /locus_tag="IWO31_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT         1510 a          869 c          582 g         1837 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc ctgaatcggc tacactagac
     4741 tagacagaaa aaataaggtg tgtagaataa taaaaaacac actggaggat ttttcatg
//
LOCUS       DACECS010000119         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.123, whole genome shotgun sequence.
ACCESSION   DACECS010000119 DACECS010000000
VERSION     DACECS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.123"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWO31_002696"
     CDS             564..989
                     /locus_tag="IWO31_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303060.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWO31_002697"
     CDS             2141..3118
                     /locus_tag="IWO31_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0303061.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWO31_002698"
     CDS             3128..3625
                     /locus_tag="IWO31_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0303062.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWO31_002699"
     CDS             4001..4282
                     /locus_tag="IWO31_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303063.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWO31_002700"
     CDS             4284..4700
                     /locus_tag="IWO31_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303064.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACECS010000120         4632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.124, whole genome shotgun sequence.
ACCESSION   DACECS010000120 DACECS010000000
VERSION     DACECS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.124"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWO31_002701"
     CDS             174..518
                     /locus_tag="IWO31_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0303065.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWO31_002702"
     CDS             519..938
                     /locus_tag="IWO31_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0303066.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWO31_002703"
     CDS             complement(1030..1380)
                     /locus_tag="IWO31_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303067.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWO31_002704"
     CDS             complement(1346..2566)
                     /locus_tag="IWO31_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0303068.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IWO31_002705"
     CDS             2932..3498
                     /locus_tag="IWO31_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0303069.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IWO31_002706"
     tRNA            3643..3730
                     /locus_tag="IWO31_002706"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="IWO31_002707"
     CDS             4064..>4632
                     /locus_tag="IWO31_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0303070.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DACECS010000121         4607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.125, whole genome shotgun sequence.
ACCESSION   DACECS010000121 DACECS010000000
VERSION     DACECS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.125"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(94..678)
                     /locus_tag="IWO31_002708"
     CDS             complement(94..678)
                     /locus_tag="IWO31_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0303071.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(889..1092)
                     /locus_tag="IWO31_002709"
     CDS             complement(889..1092)
                     /locus_tag="IWO31_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303072.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1102..1524)
                     /locus_tag="IWO31_002710"
     CDS             complement(1102..1524)
                     /locus_tag="IWO31_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303073.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1762..2376
                     /locus_tag="IWO31_002711"
     CDS             1762..2376
                     /locus_tag="IWO31_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0303074.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2541..3365)
                     /locus_tag="IWO31_002712"
     CDS             complement(2541..3365)
                     /locus_tag="IWO31_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303075.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(4003..4260)
                     /locus_tag="IWO31_002713"
     CDS             complement(4003..4260)
                     /locus_tag="IWO31_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0303076.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4253..4522)
                     /locus_tag="IWO31_002714"
     CDS             complement(4253..4522)
                     /locus_tag="IWO31_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0303077.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1514 a          811 c          623 g         1659 t
ORIGIN      
        1 aataagttat cagtagacac ttacaaaaaa ttctcatact attgtcactt ttctagataa
       61 atggcaaaaa ttttcagcat taaatgacaa aaactaatga gaatttgtct ttccattttt
      121 ctcctccatt tttgccaaat aacgataaat ggtggttctt gagacgttcc accgttccgc
      181 aatagtctta ataggtgtac cgctatccac taaagtcttc agtaattcca aatcttgttc
      241 agacaatttt tcaggacgac caccaaaccg tcctcttgca cgagcagctg ctcgtcctgc
      301 tgcggatcgt tccaggatca aattacgctc aaattctgca aatgccgcaa acaaatgaaa
      361 catgagctgt cctgtagaac ttgatttgtc catcgtaata ttttcttgaa tactatgaaa
      421 actcacacca cgctcattca gtcgattgac gattgaaatc aaatcttcca tatttctacc
      481 taaacgatct aagcgccaaa cgacaagtgt atcgccagag cgaacaaaat caatggccat
      541 ttctaatcct ggtcgattac tttttgcccc actcatatga tcagtaaaaa ttttttcaca
      601 accaaattta tttaggctgt cttcttgtaa atccaaattt tgtaaaccag tagatacccg
      661 ggcgtatcct attttcaaaa aaatcactcc ttcattaatt ttattgtaac ataactttat
      721 ccatatagtt taactgaaca tagatattgt tatgggttta tgaacatgaa atgaattgtt
      781 tttttgaaaa ataaagcaaa aaaagaaagt gtccagaaat gggtgatttt atgaacactt
      841 tcaattagaa aaaaagaatg tcgactagac actctttttt taaaatcatt atttttcttt
      901 ttgatatgtt ttttggtctc ttttttttct tttttcttca gaatcttcta gattagaaat
      961 tgatttttga attttttgtg tctcctgtaa agttcgctga acttgtaaag ctgattcttc
     1021 tttcggtaaa ttaattttac ttagaatatc gctatcttga taaaaatttg gtgtgttcac
     1081 aaattctggc ataaaaaatc cctactttct agttaaggtt cctgaataag tcgcaatcca
     1141 attgtttcca gctgatttat aactttgtaa atacaatttt ccatagtacc aataaccatt
     1201 tcttttttct ttccaattcc gtgattgagg aggtctagaa ggccaaccaa ctttatcaaa
     1261 aatcatcgtt cctgtcactc gtatcgtttt agaagctgct agagcaagta atggagaaac
     1321 tattaaataa ttttcagtaa cagttaagtc gttagttggg ttaccaatgg ctattccgtc
     1381 cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt catttgtact
     1441 atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta aaatacctaa
     1501 agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaacta agaaaacgtt
     1561 ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat aaaatatatt
     1621 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
     1681 tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
     1741 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
     1801 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
     1861 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
     1921 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
     1981 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
     2041 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
     2101 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
     2161 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
     2221 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
     2281 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
     2341 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
     2401 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
     2461 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
     2521 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
     2581 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     2641 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     2701 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     2761 aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt ttttagcact
     2821 gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc
     2881 aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca
     2941 atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa
     3001 ttctcaaata ttagagttaa atcaggtttc tcttgttgat aaaattcctt tacccctatt
     3061 tcacaggctg taattatcga aataaaccta tctctagtat ttgatatatt tttacttctt
     3121 ttaaggagta taagagaaaa ggggatactt tcgtgtccat gcatgtaatt aactaattgt
     3181 tgagcttcag aagttatatc tctaattata tagttaacgg aattaggaag tgtctttaac
     3241 tctaatgaaa tttctatcca attactatct tctaatctgt atatgctcga agtataattt
     3301 gattcgtcag caagcatcgc tacatcccaa acactgatca taaaatcttt taaacgttca
     3361 atcatttcag caatattgct aaaatcagct aatttttctt tagaataaaa aaaacattta
     3421 tatcagaatc aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat
     3481 ttatgtttat tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt
     3541 ccattacttc tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa
     3601 taaatcatta atttttagct aaattctatg agaagtttat ttttgttgtt tttgctaatt
     3661 aactaaaact agtacaaaaa atttaaaata gattattaaa cctattgctt attctattca
     3721 gtcatatata taagcgtcat cctttagtaa ctaacatccc acactacaat agtattataa
     3781 catatctatt caacttcaaa ttgttgaaat ttaagccatt tatatttact tttcctcaac
     3841 tttagaaatg attttactaa ccttatatcc gtaatacgcg taacaaacac attcattaat
     3901 aacaatcaac actaagctat attagctttt taaatgacta gggtaaataa aataaggatc
     3961 aaaaactttt aagtttctga ccctttcctt acttccgatt ggttaatagt gatcttttgc
     4021 agaataaata aatatcgttt cattttcaac tctatatatc agtctatgtt catctgtaat
     4081 tcttctggac cattttccag ataaatcatg ctttaatggc tcaggttttc ctaatccagc
     4141 aaagggggaa cgatcgatat cttttattaa cttgttaatc ttttttatat tgcttttgtt
     4201 tccttgctca tgccaataaa gataatcatc ccaagcatca tcagaccaag ccttaatcat
     4261 cagattcaac ctcgattaag tcatgtgttt taaatgcacc tttggagaat tgttcatctc
     4321 ctcgacgaat tttttccatg acgtaattat tagaaagtgt tctcaacgtt tcttgcatag
     4381 aatcataatc tctttttgat aatacaacaa ctgtatcttc tacatcttta cttgttacaa
     4441 taagtgtttc agcatcctca ttaacttgtt tcatataact acgtaaattt tggcggaaat
     4501 ttgaataagc tactgcttcc attcctttca ccccttattt atttctataa aacaattgta
     4561 cattatattg tacaattaag caaatgatta atattctaac ttcttat
//
LOCUS       DACECS010000122         4472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.126, whole genome shotgun sequence.
ACCESSION   DACECS010000122 DACECS010000000
VERSION     DACECS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.126"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            318..1049
                     /locus_tag="IWO31_002715"
     CDS             318..1049
                     /locus_tag="IWO31_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0303078.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="IWO31_002716"
     CDS             1194..3221
                     /locus_tag="IWO31_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0303079.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="IWO31_002717"
     CDS             3329..4426
                     /locus_tag="IWO31_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0303080.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          925 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt gg
//
LOCUS       DACECS010000123         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.127, whole genome shotgun sequence.
ACCESSION   DACECS010000123 DACECS010000000
VERSION     DACECS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.127"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO31_002718"
     CDS             complement(543..2048)
                     /locus_tag="IWO31_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0303081.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO31_002719"
     CDS             complement(2045..2731)
                     /locus_tag="IWO31_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0303082.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO31_002720"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO31_002720"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0303083.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACECS010000124         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.128, whole genome shotgun sequence.
ACCESSION   DACECS010000124 DACECS010000000
VERSION     DACECS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.128"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO31_002721"
     CDS             690..1658
                     /locus_tag="IWO31_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0303084.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO31_002722"
     CDS             1669..2484
                     /locus_tag="IWO31_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0303085.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO31_002723"
     CDS             2552..3262
                     /locus_tag="IWO31_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0303086.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWO31_002724"
     CDS             3298..>4425
                     /locus_tag="IWO31_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0303087.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACECS010000125         4375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.129, whole genome shotgun sequence.
ACCESSION   DACECS010000125 DACECS010000000
VERSION     DACECS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.129"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..1051)
                     /locus_tag="IWO31_002725"
     CDS             complement(77..1051)
                     /locus_tag="IWO31_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0303088.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2906)
                     /locus_tag="IWO31_002726"
     CDS             complement(1500..2906)
                     /locus_tag="IWO31_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0303089.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGFSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3127..3489)
                     /locus_tag="IWO31_002727"
     CDS             complement(3127..3489)
                     /locus_tag="IWO31_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303090.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3493..3801)
                     /locus_tag="IWO31_002728"
     CDS             complement(3493..3801)
                     /locus_tag="IWO31_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303091.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3810..4229)
                     /locus_tag="IWO31_002729"
     CDS             complement(3810..4229)
                     /locus_tag="IWO31_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303092.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4222..4362)
                     /locus_tag="IWO31_002730"
     CDS             complement(4222..4362)
                     /locus_tag="IWO31_002730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303093.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1236 a          975 c          626 g         1538 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attccgcaaa ggtttctcgc aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca acaaatccct gtattttttc tttgtattct
      241 tagctagatt tccccactca gaatgatcat aaagcaaacc tttcaccata atccgttcag
      301 taactttagt atttggcgga aaataatgtg gactagcaaa gctatccaaa ctttcatgaa
      361 ccatctcatg ccaatcagtt gcttttcccc ttcgtaactg caattctagt cgccaccatg
      421 tcgttatttc cggcggaaca attttacgtt ttctttcttg ttccagtttt ttattataca
      481 tccgaatttg tcgttcactg gcacgactac cccaatatgc cgtttctaac ttaccagaac
      541 gcccataaat tggtttaaaa gacacaggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ccgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccattacctt
      841 catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga gtgtgataat
      901 aaatattttc tttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttgtttg ctaactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaggtt
     1381 ggccaaaaaa tggactggcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc acatcgtaac
     1501 taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt ttgacgataa
     1561 aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat atccttttct
     1621 gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt ttttaatcgc
     1681 tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc cacttgtaaa
     1741 tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc tacatactca
     1801 tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt atcagcattt
     1861 ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc attcccagat
     1921 aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat atctttttgg
     1981 aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc ttcaatcttc
     2041 actctctcag aagccaccag agccttttct cgctcatttt cttctctttg cctagaaata
     2101 tacctcaact catcagaaac gctctcacgc tcttttacgg ccgtttgacg caattctgaa
     2161 atctcgctca tgatttcttt atattcgtga acatctttga ttttgttagt accgactaac
     2221 tcacgctccc aaccaaaatc tgccatcaaa cgttcaattg actcaacttc ctgattccta
     2281 aaattattcc aaatcactcg accattctct ttatctgctc gtaaaccctg ctgttgcaag
     2341 gctttgttaa aacttggttg cttttgaaca ccccgtttat acccttcagc aacaggaaca
     2401 atattcaaat gcaggtgcgg acttacttcg tcattatgaa taacagcatt gtagacggat
     2461 aaattaggat tccgttcttt aaattctttg aaatattcaa ctaaaatttt gttggcaatc
     2521 actcgatttt gttctgcttg ttgttccgat atgccagtag aaaaaccatc atcacgagta
     2581 gtacgaaaat catcaagagt accaatttgc acgataaatt ctcgctgtgg ttccaatttt
     2641 ttatccttgc gaacctttgc taaatagtcc tcgatttttc tatcggctct tttctgcttt
     2701 gcattatatt tttctacggc ttcaccgaat aattcttcat atttttcacg aatatttgtt
     2761 tgttctaaat agatattttc atccgatttt gtgaaatcaa tatgcttatg ccagtcattg
     2821 attttttggc tatcagttaa ctcacgatta ttatgagaaa tgcttgtatt ctttgttccc
     2881 tttttgaacg aaatactgaa ccccaagtta aaaccctcct tcggctgtcg ctaataaaaa
     2941 gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg
     3001 ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag
     3061 gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat
     3121 aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct cactcatctg
     3181 acttttctta ttcggtatca ctccgaataa gccgtcagat tcatcgggat aatctcgttc
     3241 gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt cgtgttgctg
     3301 atcgtcagaa atcgacgata gccaccgctc aaaatcagca aaagtttcaa acccaaaacg
     3361 cttccgaaga gccttgccca ttgcagcatt ttctttctca atcaagcgtt ttttctgttg
     3421 gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat ctgctaaagt
     3481 cattcccata aactacatct ccttttcttt ggaattaata acggaaattt ggtttaactg
     3541 cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc
     3601 taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctaaat cagcattgat
     3661 aaccgagttg agaacatcca tcattcgttc aagcatttca ctataattat cttcatccgg
     3721 caaattcaaa taatcagcta accgttcata catcaattca attctatcga ctgtttcttc
     3781 tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca ttttcgattt
     3841 tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag cagggtcaat
     3901 ttttgttgat cacgaatcat ttctgttaat tcagaaatat gtttgtcctt ctcagctaat
     3961 tgatcacgca aaaactcatt ctctttttct aaaggaaaat cactgcctgt gttattgacc
     4021 ggtaattgac cagtcaattg atcacttttt gaccgataaa ttgaccgaac aacaccaatc
     4081 aactctttat ctagcatgat aatgccattt tctttatact gagcaaggtc aaaccctgaa
     4141 ttgaccagtt gattgcgcac tgtatttttc gatacaccca attcgtcagc aagatttttt
     4201 atggtcatct tttctgacaa atcagtcatt agcttctcca ctactttcag ctaccgcata
     4261 aatatattta acggcgtgct tagggataat aatcggagag tcaattttat tatttttcaa
     4321 aatcaattca tattgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DACECS010000126         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.130, whole genome shotgun sequence.
ACCESSION   DACECS010000126 DACECS010000000
VERSION     DACECS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.130"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IWO31_002731"
     CDS             complement(387..1853)
                     /locus_tag="IWO31_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0303094.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWO31_002732"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWO31_002732"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0303095.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACECS010000127         4272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.131, whole genome shotgun sequence.
ACCESSION   DACECS010000127 DACECS010000000
VERSION     DACECS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.131"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IWO31_002733"
                     /pseudo
     CDS             1..492
                     /locus_tag="IWO31_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IWO31_002734"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IWO31_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IWO31_002735"
     CDS             1171..1617
                     /locus_tag="IWO31_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303096.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IWO31_002736"
     CDS             1619..1756
                     /locus_tag="IWO31_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0303097.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="IWO31_002737"
     CDS             1794..2087
                     /locus_tag="IWO31_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303098.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="IWO31_002738"
     CDS             2084..2308
                     /locus_tag="IWO31_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0303099.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="IWO31_002739"
     CDS             2305..3330
                     /locus_tag="IWO31_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0303100.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="IWO31_002740"
     tRNA            3520..3593
                     /locus_tag="IWO31_002740"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1484 a          729 c          858 g         1201 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     4141 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat
     4201 cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa acacactgga
     4261 ggatttttca tg
//
LOCUS       DACECS010000128         4202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.132, whole genome shotgun sequence.
ACCESSION   DACECS010000128 DACECS010000000
VERSION     DACECS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.132"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..353)
                     /locus_tag="IWO31_002741"
                     /pseudo
     CDS             complement(1..353)
                     /locus_tag="IWO31_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(613..1161)
                     /locus_tag="IWO31_002742"
     CDS             complement(613..1161)
                     /locus_tag="IWO31_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303101.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="IWO31_002743"
     CDS             complement(1162..2019)
                     /locus_tag="IWO31_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0303102.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="IWO31_002744"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="IWO31_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="IWO31_002745"
     CDS             complement(2581..2910)
                     /locus_tag="IWO31_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0303103.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="IWO31_002746"
     CDS             complement(2987..3259)
                     /locus_tag="IWO31_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0303104.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="IWO31_002747"
     CDS             complement(3259..3522)
                     /locus_tag="IWO31_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0303105.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3628..4044)
                     /locus_tag="IWO31_002748"
     CDS             complement(3628..4044)
                     /locus_tag="IWO31_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303106.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4050..>4202)
                     /locus_tag="IWO31_002749"
     CDS             complement(4050..>4202)
                     /locus_tag="IWO31_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0303107.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1593 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata cttatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatttttat taacaagttt
     3661 tatcacaggc ttcatatatt tgattataac ttcatcttct acgtagatat ctgttatcat
     3721 tacgacatct gtactatttc gattgagtac tttcacttga tctccagcat tgattttccc
     3781 tctaaatctt ttaggaatga accaactata catatgatta gcatcaccaa aatgtgcttt
     3841 tatcgcattc tcaggatata acttattcaa tagttcattc tttttaattt tcaaaacttc
     3901 aaatctatac ttatcatcat ctgctaatct aaataaatct tctaaacgca tgttttctaa
     3961 ctgtaagttt tgaaaatctt ccggtataaa ttttcggttc tgttgcgaag tttgaaaatc
     4021 aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt
     4081 tcagtagaca ccgaaaagct gaagagcgtt ccatttcttc ggttcttttt atatattcct
     4141 cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta
     4201 tt
//
LOCUS       DACECS010000129         4184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.133, whole genome shotgun sequence.
ACCESSION   DACECS010000129 DACECS010000000
VERSION     DACECS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.133"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(2..1222)
                     /locus_tag="IWO31_002750"
     CDS             complement(2..1222)
                     /locus_tag="IWO31_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0303108.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IWO31_002751"
     CDS             complement(1242..1847)
                     /locus_tag="IWO31_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0303109.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IWO31_002752"
     CDS             complement(1896..2828)
                     /locus_tag="IWO31_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0303110.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3249..4109)
                     /locus_tag="IWO31_002753"
     CDS             complement(3249..4109)
                     /locus_tag="IWO31_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0303111.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1187 a          847 c          688 g         1462 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgtttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgat aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt agttaatcaa tagtatcatt tttttcatat gatttaattc atcaattaat
     3301 tttttgtttt tattaatttt agcaaaagct agtgcatctt gaatcaattc gagcacttct
     3361 gccttcgact tatttttagc ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag
     3421 taataagtca aatggttatc cgaacaaata tcgattgcgt aatttagtag tgcatcactt
     3481 gtttccaaat caccgtcttg cgcaaaaaaa acaccacaat gaaagacgat atttaacacc
     3541 cgccagatat cttctgttgt acgaatcgga tacgtataaa ttttttcaaa cactcgattg
     3601 aaataaactt ctgctttttc caactctttt tctctagcat agaccattcc aatacctgta
     3661 tatgccaata aacgaaacaa cgtgttggct gtttctgcca tcaacaactg atcaaaatgg
     3721 aacaacacat ccgtaattgg tgcattcgta aaaatcattt caaatccttt tagatagtag
     3781 taacgtgcaa tcagctcaga ctcgtcaatt accttgggat caatggctgc taaaatctgt
     3841 gtcaattgtt catattcact cgtaatcagt gaaaactctg ctttttctag ctgttcatca
     3901 agttgtgtat tgatcacgtt catcgaagaa aaaagttcaa ctaacggaag attgattcga
     3961 ttacataatt tgattaaaat ttttaacgaa gggacttgac cattgttctc aaatttactt
     4021 aatgttgctt gtgtacaaat tccttcagac acttcttttt gagataagtt ggcagccttt
     4081 cttgccgtaa taaatttttc aatgttcatt attttctctc caatcagacc tcacacgaaa
     4141 atcatttctg tctcgatttt agcatgtttt ctttgggaat gctt
//
LOCUS       DACECS010000130         4152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.134, whole genome shotgun sequence.
ACCESSION   DACECS010000130 DACECS010000000
VERSION     DACECS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.134"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWO31_002754"
     CDS             <1..106
                     /locus_tag="IWO31_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0303112.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWO31_002755"
     CDS             970..1182
                     /locus_tag="IWO31_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303113.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWO31_002756"
     CDS             complement(1460..3259)
                     /locus_tag="IWO31_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0303114.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWO31_002757"
     CDS             complement(3383..3979)
                     /locus_tag="IWO31_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0303115.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1227 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt tt
//
LOCUS       DACECS010000131         4122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.135, whole genome shotgun sequence.
ACCESSION   DACECS010000131 DACECS010000000
VERSION     DACECS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.135"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..427)
                     /locus_tag="IWO31_002758"
     CDS             complement(152..427)
                     /locus_tag="IWO31_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0303116.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="IWO31_002759"
     CDS             complement(399..1352)
                     /locus_tag="IWO31_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0303117.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="IWO31_002760"
     CDS             1964..3457
                     /locus_tag="IWO31_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0303118.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..3888
                     /locus_tag="IWO31_002761"
     CDS             3592..3888
                     /locus_tag="IWO31_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0303119.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3998..>4122
                     /locus_tag="IWO31_002762"
     CDS             3998..>4122
                     /locus_tag="IWO31_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HAQ0303120.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNV"
BASE COUNT         1384 a          680 c          740 g         1318 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggttattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc cttagcttct
     3781 ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga gtatattgag
     3841 tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta gtttttattt
     3901 acgccgtttg tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatta
     3961 taatacccgc taaagcatac aaggagagtg aacacaaatg acttataccc atcttacacc
     4021 aaacgagctt gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc
     4081 gaagcagctt aaacgtggac gccaaacaat ttacaatgtc ta
//
LOCUS       DACECS010000132         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.136, whole genome shotgun sequence.
ACCESSION   DACECS010000132 DACECS010000000
VERSION     DACECS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.136"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWO31_002763"
     CDS             591..1280
                     /locus_tag="IWO31_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0303121.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWO31_002764"
     CDS             1267..2475
                     /locus_tag="IWO31_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0303122.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWO31_002765"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWO31_002765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0303123.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          703 c          812 g         1238 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DACECS010000133         3923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.138, whole genome shotgun sequence.
ACCESSION   DACECS010000133 DACECS010000000
VERSION     DACECS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.138"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IWO31_002766"
     CDS             complement(573..1130)
                     /locus_tag="IWO31_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303124.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IWO31_002767"
     CDS             complement(1309..1920)
                     /locus_tag="IWO31_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303125.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IWO31_002768"
     CDS             complement(2033..2290)
                     /locus_tag="IWO31_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303126.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IWO31_002769"
     CDS             complement(2887..3165)
                     /locus_tag="IWO31_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303127.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IWO31_002770"
     CDS             complement(3199..3450)
                     /locus_tag="IWO31_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303128.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACECS010000134         3915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.139, whole genome shotgun sequence.
ACCESSION   DACECS010000134 DACECS010000000
VERSION     DACECS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.139"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(153..980)
                     /locus_tag="IWO31_002771"
     CDS             complement(153..980)
                     /locus_tag="IWO31_002771"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0303129.1"
                     /translation="MVLDFIEILKVIFLGIVEGITEWLPISSTGHMILADKFITLNMS
                     EAFKEMFFVVIQLGAILAVVVMFWNKMFPFQLKDKSQPLVKKDTFSLWFKVAVACIPS
                     GIMGILFDDYLDAYLQTPIVISIMLIFYGLLFILIENWNKKRTPTTMALSDISYKTAI
                     LIGAFQVLSLIPGTSRSGATIIGALLIGVSRVAAAEFTFFLAVPTMLGASAFKLLKFG
                     FDFTSAELLALVLGMAVAFAVSVLVIKFLMNFIKKHDFKVFGWYRIVLGILVYLLYL"
     gene            complement(980..1729)
                     /locus_tag="IWO31_002772"
     CDS             complement(980..1729)
                     /locus_tag="IWO31_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0303130.1"
                     /translation="MLNLISCELLKLKRSKMVLISVAGVLSTPLLMLIEALQTHFDKP
                     EIIFTLSDIYSDSVLYIMLLVNIMIYVAIAAYLFSREYTESTLKTILPIPISRTKLLI
                     GKFCTLLLWIVMLTLVTWAGIFIACGLYHAVFTLEGYSLLVAIVWLPKFLFGSILMFL
                     TVSPFVFVAMKTKGFVAPMIGSAVIVMGSAALSNQEWGALYPWTATYFLVQGKLQSTG
                     YPTLLSVSIIILVSAVGFLMTFHHFKKEDLK"
     gene            complement(1722..2639)
                     /locus_tag="IWO31_002773"
     CDS             complement(1722..2639)
                     /locus_tag="IWO31_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0303131.1"
                     /translation="MDYIIETENLTKRYGTATVVDKVNLHVPTGKIYGLLGRNGAGKT
                     TAMKMMLQLALPTDGTVRLFGTNYKENIHTLYSKVGSIIETPGFYSNLTGYENLQILA
                     KLRGGVSKSGVEKALEVVGLHKEKRKVFSDYSLGMKQRLGIAAAIMHEPELLILDEPI
                     NGLDPIGIVEIRSFLSELSHNHGITIFISSHVLSEIEQIADIIGVMHEGHLVEEVNIS
                     ELHKRNRKYIQFDLSDSEIAGKILESHYHMTDFTVQDGTVRIYDFSQSVGEINREFAR
                     NGLLVTRIDDSEENLEDYFSKLIGGGGIA"
     gene            complement(2822..3436)
                     /locus_tag="IWO31_002774"
     CDS             complement(2822..3436)
                     /locus_tag="IWO31_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0303132.1"
                     /translation="MEFNEKLQQLRTGKNLTQEQLAEQLYVSRTAISKWESGKGYPNI
                     ESLKCISKFFSVTIDELLSGEELITLAETENRSNLKKIYNYIYGILDMMAVAFIFLPL
                     YGNSVGGYVYAVNLLSFTATTPFNLAVYWSAFAALIVIGIAKIILTHLDKETWGGIAT
                     KCSLGLTALAICFLAAAREPYITALMFLLFVAKIVVWIKQNGTK"
     gene            complement(3426..3623)
                     /locus_tag="IWO31_002775"
     CDS             complement(3426..3623)
                     /locus_tag="IWO31_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0303133.1"
                     /translation="MDYISVKAASEKWGISERRIQKLCEENRIDGTEKFGRAWMIPKD
                     AEKPVDGRMKTRNKQEENHGI"
     gene            3789..>3915
                     /locus_tag="IWO31_002776"
     CDS             3789..>3915
                     /locus_tag="IWO31_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0303134.1"
                     /translation="MYQDERKLDFKPLGIAIKKAREAKGWTQEYLAQLVDLTPRSI"
BASE COUNT         1219 a          835 c          684 g         1177 t
ORIGIN      
        1 ttggtttgtg taaacgcaaa gaaacggcgg ctctgaaaat caaagccacc gtttcatagg
       61 cgtgaaaata tgtctgctgt acgacggctt ttttcttttg ccgtcgtgcg gcagattatt
      121 gcttatggaa ttgtttttct atcacattac tgttataagt acaacaagta taccaagata
      181 ccaagcacaa ttcgatacca accaaacacc ttaaaatcat gttttttgat aaagttcatt
      241 aggaatttaa ttactaagac agataccgca aatgccacag ccataccgag aacaagagcg
      301 agcagttctg cacttgtaaa atcaaacccg aattttaaca gcttaaaagc acttgctcca
      361 agcatagtag gtactgcgag gaaaaaagta aactctgctg ctgccactcg tgaaactcct
      421 atgagtaaag cacctataat cgttgctccc gaccgtgatg tgccgggtat aagtgataac
      481 acttgaaatg cccctatcaa gattgctgtt ttatagctga tgtcagaaag tgccatagta
      541 gtaggagtac gctttttatt ccaattttct atgagaatga ataacaaacc ataaaagatt
      601 aacatgattg aaatcacaat gggggtttgg aggtaagcat ccaaataatc atcaaataaa
      661 atccccataa tacctgacgg tatacaagcc actgcaacct tgaaccacaa cgagaaagta
      721 tcctttttaa caagtggctg cgatttatcc tttagctgaa atggaaacat cttgttccaa
      781 aacattacaa ccactgcgag tatagctcca agttgaataa caacaaaaaa catttcttta
      841 aatgcttcgc tcatattaag tgtgataaat ttgtccgcaa gaatcatatg tcctgtgctg
      901 ctgataggta gccactcagt aataccctca acaattccaa gaaaaataac ttttaaaatt
      961 tctataaaat caagtaccat tatttcaaat cctccttttt aaaatgatga aaggtcataa
     1021 gaaaaccaac tgctgataca agaataataa tagatacaga cagcagtgta ggatagccgg
     1081 tactctgaag tttaccctgc accaagaaat aggtggctgt ccacggatac aacgctcccc
     1141 attcttgatt tgaaagtgcg gcacttccca tgacaatcac ggcagagcca atcatcgggg
     1201 cgacaaatcc ttttgttttc atagcaacaa acacaaaagg agaaactgtt aaaaacatca
     1261 ggatactgcc aaacaaaaac ttggggagcc atactattgc cactagtaag ctatatccct
     1321 ccaatgtaaa gacagcgtga tatagcccac aagcgataaa tatacctgcc catgttacaa
     1381 gggtgagcat aacaatccaa agaagcaggg tgcaaaattt tccaattaat agttttgtcc
     1441 ttgaaatggg tatgggcaat atggttttga gtgtgctttc tgtgtactct ctgctaaaca
     1501 agtaagctgc aattgccaca tatatcataa tgtttaccag cagcatgata tacagtacac
     1561 tgtcgctgta tatgtcagac aaggtaaaga taatctccgg cttatcaaaa tgtgtttgca
     1621 aggcttctat taacatcaaa agtggggtag ataatacccc tgccacacta attagcacca
     1681 tttttgaacg ctttagtttt aataattcac aagaaataag attaagcaat accgccacct
     1741 ccaatcagtt tagaaaagta gtcctctaag ttttcctcac tatcatctat ccttgtcacg
     1801 agcagtccat ttcgtgcaaa ttctcgattg atttctccaa cactttggct aaagtcataa
     1861 attctcaccg taccgtcctg cactgtaaaa tccgtcatgt ggtagtggct ttctaaaatc
     1921 tttccagcta tctcactgtc agataaatca aattgaatgt attttcgatt ccttttgtga
     1981 agctcggata tattcacttc ctctactaaa tgcccctcgt gcataacgcc gataatatct
     2041 gctatctgtt caatctcgct taaaacatgg cttgagataa aaatggtaat gccatgattg
     2101 tgactaagtt cagataaaaa tgagcgtatt tcaactattc caatggggtc aagtccgtta
     2161 atcggttcgt caagtattaa aagctccggc tcgtgcataa tggcggcggc aataccaagc
     2221 cgttgcttca ttccgaggga ataatcggaa aagacttttc ttttctcttt atgcagcccc
     2281 acaacttcaa gagctttttc tactccactt ttagatactc cccctcgcag tttagcgaga
     2341 atttgcaaat tctcataccc tgttaaatta ctgtaaaatc ccggtgtttc gataatagag
     2401 cctactttgc tataaagggt atggatattt tccttatagt ttgtgccaaa caagcgtacc
     2461 gttccatccg ttgggagagc aagctgcaac atcattttca ttgctgtggt tttgcctgct
     2521 ccgtttctgc cgagcaaacc ataaattttg cccgttggca catgaagatt gaccttatcg
     2581 acaacggtgg ctgttccgta tcgcttcgta agattttctg tttcaatgat ataatccata
     2641 tttgattttc ctttccgttg gttgctgttc ttgttttcac tgaccacaat tttattatca
     2701 caggagctat atcaaaagcc aagaaaccta ccgtcacatc gcctacatct atcatgtcaa
     2761 catgaaaaaa agccccgaca cttatcgtgt cagagcctat ttcaagggat ttatatcgtt
     2821 tttactttgt tccgttctgc ttaatccaaa caactatttt tgcgacaaac agcagaaaca
     2881 taagtgctgt tatatatggt tctcttgctg ctgctaagaa acagatagcc aaagcagtga
     2941 gtccaaggga gcattttgta gcaatgccgc cccatgtttc tttatctaaa tgggtaagta
     3001 ttattttagc aatccctatt acaatcaagg cggcaaaagc actccagtag actgcaagat
     3061 tgaatggtgt ggtggctgta aatgatagta gattgaccgc ataaacataa ccgccaacag
     3121 agtttccata taacggaaga aatataaacg caactgccat catatccaat attccataaa
     3181 tatagttgta aatctttttc aagttggaac gattttcagt ttcggcaagt gtaatcagtt
     3241 cttcgcccga tagtagttca tctatggtca cagaaaagaa tttagaaata cacttgagcg
     3301 actcaatgtt agggtaaccc ttgccgcttt cccattttga aatggctgtt cttgatacat
     3361 ataattgctc cgcaagttgt tcctgcgtta agttctttcc agtcctaagc tgttgtagct
     3421 tttcattaaa ttccatgatt ctcctcctgt ttgttccttg ttttcatacg tccatcaacg
     3481 ggtttttctg catcttttgg tatcatccat gcacgaccaa atttttcagt tccgtcaatg
     3541 cgattttcct cacataattt ttgtatccgt ctttccgata tgccccattt ttcagaagcg
     3601 gctttcacag aaatgtaatc cataatatca acttcctttc gcaaagagta tataattatt
     3661 atatacggaa tgacgaataa tagcattttt ctctccacta atacgaaatt atacactact
     3721 aaacataaaa tcatacttcg ttcaaatatc atcacccgaa atgtacaatg atatagggtg
     3781 atattaaaat gtaccaagat gaaagaaaac ttgattttaa gccgttaggt atagcgataa
     3841 aaaaagctcg ggaagcaaaa gggtggacac aggaatatct tgcccaactg gtagacctta
     3901 cgccacgctc tatta
//
LOCUS       DACECS010000135         3886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.140, whole genome shotgun sequence.
ACCESSION   DACECS010000135 DACECS010000000
VERSION     DACECS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.140"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..521
                     /locus_tag="IWO31_002777"
     CDS             <1..521
                     /locus_tag="IWO31_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0303135.1"
                     /translation="SSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPK
                     PVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIG
                     GWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGS
                     VQWFAPDFAVSM"
     gene            complement(589..2388)
                     /locus_tag="IWO31_002778"
     CDS             complement(589..2388)
                     /locus_tag="IWO31_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0303136.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            2511..3017
                     /locus_tag="IWO31_002779"
     CDS             2511..3017
                     /locus_tag="IWO31_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0303137.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            3096..3818
                     /locus_tag="IWO31_002780"
     CDS             3096..3818
                     /locus_tag="IWO31_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0303138.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT         1168 a          727 c          817 g         1174 t
ORIGIN      
        1 aatcttcaac aacagaagaa tcaacaacac ctgcgcctac aacaccatca acagatcaaa
       61 gtgttgatac aggaaacggc acaggaagta gtactccggc tccaacgcca acaccaacac
      121 ctgaacaacc aaaacctgta acacctgctc cagctccttc tggaagtgtg aatggtgcag
      181 caatcgtagc agaagcttac aaatatattg gtactccata tgtttggggc ggtaaagatc
      241 caagtggctt tgactgctca gggttcacac gctatgttta catgcaagta actggtcgtg
      301 acatcggtgg ttggacagtt cctcaagaat cagctggaac aaaaatttca gtttctcaag
      361 caaaagctgg agacttgtta ttctgggggt cacaaggcgg aacttaccac gtagcgattg
      421 ctttaggcgg aggacaatat atccatgctc ctcaaccagg cgaaagcgta aaagttggtt
      481 cagttcaatg gtttgcccct gactttgctg tcagcatgta agaatgatta atcaaaaaga
      541 gttagcccat tctggactaa ctcttttttt attgtgataa aatagaaatc agataaattc
      601 ttctgtcaat tccatgaacg tttggatatc ttcagtcacc atgtcgatac ctgcttgcca
      661 aaaatctggt tgggtaagat cgacattcaa atgtttcttc gcaagatctt ccgtcgtcat
      721 tgaggcagta tcgcgtagta aatggatata ttcttcttca aaatttgctc ccttttggtt
      781 ggcgtaagca tagatgccca aactaaagag gtagccaaat gtgtatggga agttataaaa
      841 cggtacgtca tcaataaaga aatgcaattt tgcagcccag aaatatggat ggtaagtagc
      901 taaagctcct tgatagcctt ctttttgcgc ctcaaccatc atctctgtga tcttatcttc
      961 tgagactaat ccttcttgcc gtgcttcata gaatcgattt tcaaaaatga atcgggaatg
     1021 gatattcatg aacatagcta aagcattttg cagttttgta tctagcaaat tgattttttc
     1081 ttcttttgtt tttgcgactt ttaatgtagc atcggctacg atcagttctg cgaatgtact
     1141 tgctgtttct gcgacattca tggcatattc gcgatttaaa gaaggtaaat cccacatcgt
     1201 gctgctatgg aaagcatgtc ctagttcatg agcaagtgtc gcaacctcat taacagaatt
     1261 actgtaggtc ataaagatcc ttgattcttt tgtttctggt aactcagtac aataaccacc
     1321 aggacgtttc ccaggacgat cttctgcttc gatccagctc ttttcaaaag cagattgggc
     1381 aaatttagcc atttttggac tgaatttgtt aaagttttcg ataataaatg ctgctgcttc
     1441 gtcaaaacta aaggtttttt ctttcaaatc gcctaaaata ataggtgcat cttgatcttg
     1501 ccattccatt ttttctttac cgaaaagatt agcttttcta gtcaagtaat taacaagggg
     1561 ttgtttgttt ttctggattg tatcccacat gacagacaat gtttctttct tcaaccgatt
     1621 gtactctaat ggtttttgta gaaaatctgt gacaccatga agttcatagt cagaaagtct
     1681 aaaaccatct aaatgattca aagtatccgt gaagaggggt gccttttctt tccaggcttc
     1741 ttcccatgct gcgaaaagtg tttctctgac ttttggatct ggatcgccca tcatcttatt
     1801 gaaggcttgt ccagcagata attcgactac ttgtccatct tgttcgaagg gaatcgaaat
     1861 gctggctaca atcgtatcat aatgactgct ccaagcattt agaccatcta aagaaagcgt
     1921 atttataatg ttttcttcag cttctgataa taattgtgag ccatcacgac gaatctcgtt
     1981 taaacgaaaa gcaattgttt caaacgaaga ttgagaaagc atctgattcc aatcattgtc
     2041 agatatttct gcaaatttct ttgacaatac agtttcggct gattgcagac gaggcagcat
     2101 tgcgtaaagc ttgcctgata agacctttgc atctgaatca ttgacattgg cagaaagcaa
     2161 tgcagtaata tagctgctgc attgggtaaa accattcgta atcgtttctt gcaattgaag
     2221 aatagcgttt aactgctctg cattgtcaga actaggtgac cattttgtta cgcggtgata
     2281 atactcgttc atttggtctt ccaattgctt catccgttgg ttcaaagcat cagaatgact
     2341 gcctccggaa aaaatagaat ctagattcca atttaatgaa taactcatga aaaaactccc
     2401 ttcatattgt aatacaaaat taagtatagc acttttttat ggaagaagtc tctatgaaaa
     2461 tgattgttgt ttcttcaaaa aaagtctaag ataaaataca gaggtgactt atgaattctt
     2521 caaaaaaaca aatgaaaaac ggaaatattt atttagtaat cgcactgata gtcgttataa
     2581 tcttatttta cagttcttct caaacttacg gacagcaatc acaagtttcg cgattagaaa
     2641 gctggctgcc taatcagcca tttaaagaca cattagcggg tatacgtttt aattatggtg
     2701 gaagtatggt gagtatcaac catctgggct acgccaaatt tttagagttc tttttaagga
     2761 agggcgcaca tttcggtacc tattttattt tgggaggcag cttgttttta ggtgttttcc
     2821 ccaagctaaa aatttggtgg ctgacagcga tccttgcttg gctttcagca acagggtacg
     2881 caggtttgga cgaattccat cagatgatga ccggtggacg aacacctatg ttccaagatg
     2941 tcatgttgga ttcgatcggt gctctgactg ctgtgatatt atgtgtgctt tttgcggcat
     3001 tcaagaagcg tttatagatt ggctgtggct ttgttttttt gtgaaatagt actagaatag
     3061 aagtgtattc tcaaaagaac aagggaggaa aaatcatgtc aggacatagt aaatggcata
     3121 atatccaagg aagaaaaaat gcccaagatg caaaacgagg gaaagtcttt cagaaacttt
     3181 ctcgtgaaat ttatatggca gctaaagcag gcggtcctga tccatctatg aatccagctt
     3241 tgcggcttgt tgtggacaaa gcaaaagctg caaatatgcc taacgacaat gtggaacggg
     3301 cgattaaaaa ggctacgagt gccgtagatg aaacgaatta cgatgaaatc acgtatgaag
     3361 gatatggtcc gggtggagta ggaatcttag tccatgcatt gacagataac cgtaatcgga
     3421 cagcgacaaa tgtccgagta gcttttactc gaaacggtgg atcactggga gaaacgggtt
     3481 cagtcagtta cttgtttgac cgcaaaggat atatcgctat tgaacgcgat gggttgactg
     3541 ttgatgaaga gacgatgttt gaggatgtat tagaggcggg ggcagaagat ctgcaaactt
     3601 ctcctgaagt attcgagatt tatactgcac cagaagattt tgctgctgta cgagatgagt
     3661 tggaaaaaga gttcactttg gcgcaagcag aattgacgat gattccacaa acgacgattg
     3721 atttaaatga ggaacaaaaa gagcaattgg aacgtctagt agataagcta gaagacgatg
     3781 atgatgtttc agaagtcttt actgctgctg aaatgtaagt agcacattgt tagataataa
     3841 aaagaaagca cctgtaccaa tcgtacgggt gctttctttt tatata
//
LOCUS       DACECS010000136         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.141, whole genome shotgun sequence.
ACCESSION   DACECS010000136 DACECS010000000
VERSION     DACECS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.141"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWO31_002781"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWO31_002781"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0303139.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWO31_002782"
     CDS             complement(2260..2511)
                     /locus_tag="IWO31_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0303140.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWO31_002783"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWO31_002783"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0303141.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWO31_002784"
     CDS             3461..3679
                     /locus_tag="IWO31_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303142.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACECS010000137         3682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.142, whole genome shotgun sequence.
ACCESSION   DACECS010000137 DACECS010000000
VERSION     DACECS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.142"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..621
                     /locus_tag="IWO31_002785"
                     /pseudo
     CDS             1..621
                     /locus_tag="IWO31_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1515
                     /locus_tag="IWO31_002786"
     CDS             1069..1515
                     /locus_tag="IWO31_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303143.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="IWO31_002787"
     CDS             1517..1654
                     /locus_tag="IWO31_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0303144.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="IWO31_002788"
     CDS             1691..1984
                     /locus_tag="IWO31_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303145.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="IWO31_002789"
     CDS             1981..2205
                     /locus_tag="IWO31_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0303146.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="IWO31_002790"
     CDS             2202..3221
                     /locus_tag="IWO31_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0303147.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="IWO31_002791"
     tRNA            3410..3483
                     /locus_tag="IWO31_002791"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1261 a          647 c          762 g         1012 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat
     3601 taatgaaagg atgtttcaca tagttatatt tctgtatatt tgaaaagttt tactttgatt
     3661 tttaaataga aagacatttg gg
//
LOCUS       DACECS010000138         3199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.143, whole genome shotgun sequence.
ACCESSION   DACECS010000138 DACECS010000000
VERSION     DACECS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.143"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            301..585
                     /locus_tag="IWO31_002792"
     CDS             301..585
                     /locus_tag="IWO31_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303148.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IWO31_002793"
     CDS             588..1004
                     /locus_tag="IWO31_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303149.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IWO31_002794"
     CDS             complement(1141..1860)
                     /locus_tag="IWO31_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0303150.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="IWO31_002795"
     CDS             2513..2608
                     /locus_tag="IWO31_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0303151.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="IWO31_002796"
     CDS             complement(2910..3155)
                     /locus_tag="IWO31_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303152.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DACECS010000139         3137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.144, whole genome shotgun sequence.
ACCESSION   DACECS010000139 DACECS010000000
VERSION     DACECS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.144"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1886)
                     /locus_tag="IWO31_002797"
     CDS             complement(<1..1886)
                     /locus_tag="IWO31_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0303153.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPD"
     gene            complement(1900..3117)
                     /locus_tag="IWO31_002798"
     CDS             complement(1900..3117)
                     /locus_tag="IWO31_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0303154.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
BASE COUNT          850 a          680 c          528 g         1079 t
ORIGIN      
        1 aaatcaggat cctccatcgt tcgtccttca cggataggct gattgggaaa ctcttttatt
       61 aaccaatcag attctttcgt catctctttg atggcccgat aatacttttt ggccatatca
      121 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
      181 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcctctgct
      241 tcaaaaacag tagggtccaa ataactttct tttttgacag actccatttg atcgatcata
      301 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
      361 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
      421 accgagccat cttctaatgc attcttgatg gtataggtgt gcaagacctc tccatactga
      481 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
      541 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
      601 tcagcactta acgctcgatg acactcatca actacaaaca cgatatgttg ccccattaat
      661 ttttggaagc gattggttcc ttttttctct tcctgtttct ttgcatattt caacgccgca
      721 tctagttttt gacgggttgt aatgatcaca acattcgcat tggcatctgc caacagagtt
      781 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcattacca
      841 gaagaaaccc cagtattaaa ctccgaagca aatttcgtaa actctgtggt ggtctgatta
      901 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
      961 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     1021 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgcac ttgataagga
     1081 tgtaacacca tgagggtttt attgtcttga tcttcactga caatcgtata atccgcaatc
     1141 aagcgatggg catctgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     1201 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc cttcggtaag
     1261 gcatttgcaa aataccgagt cgtttgttca ttggaaacca caaacaactg taaggtcgaa
     1321 aagatattgt tccgaaacat gccttcttca gcatattttt tgatttgatt aaacgcctga
     1381 taaaaaccat cttttgcagt tacttgtttt agctcgattt ggataatcgg taagccattg
     1441 atcaataagg tgacgtcaaa acgtcgatcc cgcgcttcta tgttacttcc acgttttgca
     1501 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     1561 ataagtgaga cagaacccaa ttgcggattt tcccgttcaa tcgtgatccg agccatccca
     1621 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgtccgtag caataattcg
     1681 gttttgatcg tttcaaactc tttatcggtc aaaggagaat cattcagttc tgcttgatta
     1741 tttgagatga ttttttgacg taaattgctc caaagatctt cttctgattt tagatccgga
     1801 cgatacgtcc actgattatg tccttccccg aggacacgaa tcaactgttc ttcgacttcc
     1861 gcttcctcac ggtgactaat tttcattctt cttccctccc taaacaaaca tcttttgcaa
     1921 gaagcctttt ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatcgtatc
     1981 atctagctgc ttgaagaagg caccaatttt ttgttgttca tttatatccg gtggaaactg
     2041 aaatgtcctt tgttctatac cacctttagt cacatgtacc attgtcgttc cgttagttga
     2101 ttctttaatt ttctctttat cagtcaatag ccaagtatat agatactgtt tgtccaattc
     2161 cttagataaa aatttaagtt tccaaatatg ataatggtag attacacgtt cttcattcca
     2221 catttcagga ccgaagcttg ttgcccataa atataagaga tctcctttat tagcatactt
     2281 gtcttcctct aattctaagt ttgaataata ccatctatca ttagtattaa aattacctac
     2341 tcttaaaacc ctatattttc cagaatcaag taattcttgt tgtttaaaag ctctaccatt
     2401 caaaaaaact accacttccc ccaacttccg ctcttcccaa tctcccgtaa atcctggaaa
     2461 acgaacttct ggcacttttg ctccgttttt agggaacatt ttttgtaaga atcctttttt
     2521 cgtttccttc agcaaatcta attcgtgctg gtgaagcatg atggtgtgat caagttgttt
     2581 gaaaaatgaa ccgatcttga tctgttcgtc ataagttgga agtgagatag gtgtcttttt
     2641 accactatca attgtatagg ttccgacggt tccaatgcct gcaacgtcac ggatatactt
     2701 ctgaattttg ttatttatga acgagtagta aaagaagttt ccgtcaattt tatgctcatt
     2761 cttgaaccaa atgatgtttc catccttaaa gtaaattggg ttatcatcag tgattaataa
     2821 ggggacacct attgatccga caccagttac aagcaagtct ccttgagaaa ctttacctga
     2881 atttttcgaa tattcattat atttttcttc tgaaatgtaa agataatcgg atggttcctc
     2941 attttttgca gctgagacaa tatcacgagc acgtaaaaat ctaatacctg agttagtcca
     3001 atctgattga tgaattcgct tcacagaagt aacatccatc atatctccta acttacgctc
     3061 ttcccaatct tccgtaaaac caggaaaccg gatttctggt cgtgtatcat tgctcatgtt
     3121 tgaacacctc cagcgca
//
LOCUS       DACECS010000140         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.145, whole genome shotgun sequence.
ACCESSION   DACECS010000140 DACECS010000000
VERSION     DACECS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.145"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1243
                     /locus_tag="IWO31_002799"
     CDS             <1..1243
                     /locus_tag="IWO31_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0303155.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="IWO31_002800"
     CDS             1256..1888
                     /locus_tag="IWO31_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0303156.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="IWO31_002801"
     CDS             2093..2320
                     /locus_tag="IWO31_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0303157.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="IWO31_002802"
     CDS             2389..2655
                     /locus_tag="IWO31_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0303158.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="IWO31_002803"
     CDS             2645..2914
                     /locus_tag="IWO31_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0303159.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DACECS010000141         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.146, whole genome shotgun sequence.
ACCESSION   DACECS010000141 DACECS010000000
VERSION     DACECS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.146"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IWO31_002804"
     CDS             complement(106..1053)
                     /locus_tag="IWO31_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0303160.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IWO31_002805"
     CDS             1299..1763
                     /locus_tag="IWO31_002805"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0303161.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IWO31_002806"
     CDS             complement(1825..2868)
                     /locus_tag="IWO31_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0303162.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          989 a          593 c          571 g          839 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACECS010000142         2886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.147, whole genome shotgun sequence.
ACCESSION   DACECS010000142 DACECS010000000
VERSION     DACECS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.147"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            798..1064
                     /locus_tag="IWO31_002807"
     CDS             798..1064
                     /locus_tag="IWO31_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303163.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="IWO31_002808"
     CDS             1122..1634
                     /locus_tag="IWO31_002808"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303164.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="IWO31_002809"
     CDS             1631..2668
                     /locus_tag="IWO31_002809"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0303165.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DACECS010000143         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.148, whole genome shotgun sequence.
ACCESSION   DACECS010000143 DACECS010000000
VERSION     DACECS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.148"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..1054
                     /locus_tag="IWO31_002810"
     CDS             95..1054
                     /locus_tag="IWO31_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAQ0303166.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="IWO31_002811"
     CDS             complement(1487..2059)
                     /locus_tag="IWO31_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0303167.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="IWO31_002812"
     CDS             complement(2075..2680)
                     /locus_tag="IWO31_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0303168.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          885 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 g
//
LOCUS       DACECS010000144         2739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.149, whole genome shotgun sequence.
ACCESSION   DACECS010000144 DACECS010000000
VERSION     DACECS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.149"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWO31_002813"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWO31_002813"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0303169.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DACECS010000145         2668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.150, whole genome shotgun sequence.
ACCESSION   DACECS010000145 DACECS010000000
VERSION     DACECS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.150"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..318
                     /locus_tag="IWO31_002814"
     CDS             <1..318
                     /locus_tag="IWO31_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0303170.1"
                     /translation="KAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIG
                     KRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHL
                     VKK"
     gene            732..1550
                     /locus_tag="IWO31_002815"
     CDS             732..1550
                     /locus_tag="IWO31_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0303171.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1623..1982)
                     /locus_tag="IWO31_002816"
     CDS             complement(1623..1982)
                     /locus_tag="IWO31_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0303172.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          924 a          372 c          471 g          901 t
ORIGIN      
        1 aaagcaatca ttcctgagaa cttagatgag gttaaacttt atttatggga agataacgga
       61 gaaattgtcg gttttagtgg tgtaagtgca gaagaattag ttatgctatt tcttgatcca
      121 gattttattg gtaagcgata tggtagtcaa atattaattg agctgattga aactgaagat
      181 ataaaaagaa ttgatgttaa tagtcaaaat aaatatgcaa aacattttta tttaagccat
      241 ggatttgaaa ttgaatctga agaagactta gatggttttg gtaaaccata ccctataacg
      301 catttagtaa aaaaataatt catgaatggt ttattacctg attgaacatg aataaaagta
      361 atatttttat tttgatggcc tatttctagg ttatgaatta tccagatcaa atttgtgtat
      421 tacattcagt aataaggtgc ttatcttagg atcaacttat gatactagct gaccaaggat
      481 gtaccagtta tagaaaatca gttaaagtaa aaatgataaa aatcttatgt caacactgat
      541 ttaggctgac ctgtgggtta ggagaaagac aggtcggtag cttttaatac aaaaaatggc
      601 ttgtatgtat ctagatagtt aactgaaatt agaaatgttc ttatttttaa cggagtaaga
      661 aaaataggct gctttaggtc tgaatacttt ttcaatataa tgaagatatc aaaggcgaca
      721 ggagtgtttt tatgaataag aatcctcata atctaaagtt ggtagcagta gatatggatg
      781 gaacatttgc tcgtactgat tacacatacg atatcccaag gttcaaggct gttttatcgc
      841 gaatgaaagc tgttgattgt caatttgttg ttgcaagtgg aaatcaatac taccaattga
      901 gatcgttatt tccaaattat tatgatgaat taagttttgt tgctgaaaat ggtgcttttg
      961 ttaaagataa aacagaggta ctattttcag ccgatatgcc tagaaaagcg atcgaggtgg
     1021 tccttgattt atgtaaagac tatcctgaag taaaaaatgt gatgtgtggt ctgaatagtg
     1081 cctactgtga aagaggaaag gtgagtcaag cattttttga tctaaccaat atctattacc
     1141 atagattaga atgggtagct gattttgcag aagttcaaga taaaatttta aaatttgctc
     1201 cgactatgcc tgttgaaaaa accgaatttt acgttgaatt gttccgtgaa aaattagccg
     1261 ggctattagt acctaccagt agtggtcatg gttctgtaga cttgattctt tctgagtgcc
     1321 ataaaacttt cggcttgaaa atgttagttg agcgcttggg gattaatcct gaccagtgcg
     1381 ttgcttttgg agatgggggc aatgacattg agatgttgga gtattgtggt ctgagttatg
     1441 aaatggataa tgcaacggaa gctgttaagc aagttgctaa gcatcaatgt ccttcaaatg
     1501 acgaagacgg tgttttagtg acgctcgatc gattgttccc taacacgtaa acactaggta
     1561 attcatcaaa aaacctcgat ataagaaata ttcgaggttt taaaactata tttagaacag
     1621 atttacgaag cgtctgatga gtcaaaatag acttggacat tgctatctaa aataccactt
     1681 tcattcttta aacgaattgt ttgatttgat ggagttacct ctaatactaa atgcccatta
     1741 ataaaggtag tatcgtattc agatgtttta actgttgctt cttcgcaagg tattgttaaa
     1801 tcatatgctg gaatataatg ttgtgtggaa actaataaaa aatgatcacc aatcatacag
     1861 catccctctg ctgaagaaaa ttgattagag ccatcattta aagttaacaa gaatccatcc
     1921 tttaaacgag gtacttgctt aaatacttct ggttgtactt ctaaaaataa attatcgctc
     1981 attttcacga ccaccatcga cttccacagt tgaaattgac attttattta acacacctat
     2041 tcccccttaa ctataactca attctatata aaaaatattc tattctcaaa taattgaact
     2101 tttcaaatcg ccaatttact tcggctttat cagagctcat tcatatcaag agcgattaac
     2161 gctgtaaaaa ttaaaccgaa gatctgataa attttctcta ttttctacaa tatttaaaac
     2221 gcttccttag tctattatat atctatttct cttaggagta tgtttagatg tttgaataat
     2281 agttgcccaa ctttaaaggt tattcttata atattttact tcattttcca atagaaaatt
     2341 caaaggatta ctggtttttc taaaatagtt tagtttaaat acataagtat cctccgacac
     2401 tgtattagtt cataatcatc taaatacaaa attttcaaat aaaaatattt caagctctat
     2461 cgttgacgaa tgtaaacgat acttataaag taatagaaat tgattacatt tgtaaactta
     2521 aaatggttaa tttatctcac ataaaactaa gaataaaata atgattaaaa gtggttagtt
     2581 tttatacgat ttagcctatc ggtaaaaatg aattagagag tgtaaaatat tttgtgtaaa
     2641 tgaaaaaatc catacaaaaa aggaagtc
//
LOCUS       DACECS010000146         2473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.152, whole genome shotgun sequence.
ACCESSION   DACECS010000146 DACECS010000000
VERSION     DACECS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.152"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO31_002817"
     CDS             7..624
                     /locus_tag="IWO31_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303173.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..>2473
                     /locus_tag="IWO31_002818"
     CDS             1644..>2473
                     /locus_tag="IWO31_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0303174.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          905 a          326 c          397 g          845 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt tta
//
LOCUS       DACECS010000147         2455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.153, whole genome shotgun sequence.
ACCESSION   DACECS010000147 DACECS010000000
VERSION     DACECS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.153"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWO31_002819"
     CDS             132..344
                     /locus_tag="IWO31_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303175.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWO31_002820"
     CDS             506..1024
                     /locus_tag="IWO31_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0303176.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWO31_002821"
     CDS             1031..1543
                     /locus_tag="IWO31_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303177.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWO31_002822"
     CDS             1814..2437
                     /locus_tag="IWO31_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0303178.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          409 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaag
//
LOCUS       DACECS010000148         2429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.155, whole genome shotgun sequence.
ACCESSION   DACECS010000148 DACECS010000000
VERSION     DACECS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.155"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1281)
                     /locus_tag="IWO31_002823"
     CDS             complement(<1..1281)
                     /locus_tag="IWO31_002823"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0303179.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK"
     gene            1406..2191
                     /locus_tag="IWO31_002824"
     CDS             1406..2191
                     /locus_tag="IWO31_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAQ0303180.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          818 a          455 c          397 g          759 t
ORIGIN      
        1 tttattctga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
       61 agtttgacga ttcttcttca atacaatcac cgtcgttgga atcgatgtac caaagaacaa
      121 gttcgcaggc ataccgatca cagcataaat actgccgtct tctaataatt tttgacgaat
      181 gaccccttct gcagcaccac ggaataaaac cccgtgtggc aagacgatag ccatagtccc
      241 tgtttccttc aaatgataga aaccatgaag aagaaacgca aaatctgctt tggattttgg
      301 cgctaatttc ccgtagcgat taaaccgaga atcatctaag aaggtcgtat ctgctgacca
      361 attagccgaa taaggtggat tcatcactac tgcatcaaat gtgtaaggtt catctgttgg
      421 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcat ctaccccatg
      481 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
      541 tttaacgtta tctggatgag ttaagtagtt acgtacattt aacatcaagg aacctgaacc
      601 catggttggg tcaaagacac taaagaatcg tcgttctttt tgatccaacg tcacgatttg
      661 tgccatcata tcagaaacca tatgaggcgt gtaaaattct ccggctttct tccctgcttc
      721 tgaagcaaat tgactgatca gaaattcata cgcatccccg atcacatccc catcgtgacc
      781 taaaacttcc acgtcattca atttcttgat aacttcggta atcgtcacat ttcgttgttg
      841 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
      901 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
      961 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgagatcg aataccccaa
     1021 aatatcaacg attgtggcaa tcaggtcttc ttttgattct tcatctgaaa gtaattcacg
     1081 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     1141 aaccacttgt tccaacaaac ggtcggataa atatttatag aaaatcagtc ccaataaata
     1201 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
     1261 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     1321 ccctcttatt ttagcatact cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt
     1381 cttgataaca agctacgtga cgtatatgcc taacaaaaat agaacttttg aatcaatctt
     1441 aaatgaaggt ttaaccacag aaccggcatc cattattttt gatgaagtta tcgatgacgt
     1501 tgaagatagt aagtatcata aaaatttaaa agaacttttt tattttgcac tcaacaatgg
     1561 accaagtttg ttcactggta tatcagtacc tgatagtgat aatccattaa ttatggcaaa
     1621 aatttactta acaaaatggt gtaataaata catcagggat aggaataacc cagcattaaa
     1681 aaaacctctt aaaacatttg gcgaaaaaga tgctgcttta actactagag tagcagcaaa
     1741 tgctaatatt gatgatcaaa aagtattgaa tgactacttg cgtggacatt ttttatatat
     1801 gagcgctgag aacatgaacg gaagtatttt agaggaatat ttagccgaag tgttagaacc
     1861 tgaaggttgg atttggtgcg ccggctctgt atatcgagct gttgatttct gttatttagg
     1921 aacctcacca attcttttac aagtgaaaaa taaatacaat acagaaagca gttcgagttc
     1981 agctataaga gtaggaacaa caattagaaa atggaatcgt ttaaataagt cgacaaaaat
     2041 ttctggtttg gatagcccta tccccaattg gaaagcatta atagaaatga ctgaagctag
     2101 taaggaactg gcagctaagt taactgaaaa tagttattta gcttatatta atgaaaaatc
     2161 cacacgagaa ttatggacac tcgatgacta acaatatcct tattagtatc tatccacgat
     2221 acatggaaaa aattataaac aaacaaaaaa actttgaatt tagaaatttc tatcttacct
     2281 cagatcctta taatatcttt tggatttacg aaacaatccc caccaaagct ttaaagtata
     2341 aattgattgt aaaaaatcca attactaaac ttaagaaaaa tcaacactac tttttaggag
     2401 acgatgagtt tgaacaatta gtttaaaag
//
LOCUS       DACECS010000149         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.156, whole genome shotgun sequence.
ACCESSION   DACECS010000149 DACECS010000000
VERSION     DACECS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.156"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWO31_002825"
     CDS             5..1150
                     /locus_tag="IWO31_002825"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0303181.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWO31_002826"
     CDS             1321..1779
                     /locus_tag="IWO31_002826"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0303182.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IWO31_002827"
     CDS             1938..>2407
                     /locus_tag="IWO31_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0303183.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACECS010000150         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.157, whole genome shotgun sequence.
ACCESSION   DACECS010000150 DACECS010000000
VERSION     DACECS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.157"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWO31_002828"
     CDS             207..2069
                     /locus_tag="IWO31_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0303184.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWO31_002829"
     CDS             2069..>2398
                     /locus_tag="IWO31_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0303185.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACECS010000151         2300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.158, whole genome shotgun sequence.
ACCESSION   DACECS010000151 DACECS010000000
VERSION     DACECS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.158"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWO31_002830"
     CDS             complement(45..1364)
                     /locus_tag="IWO31_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0303186.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWO31_002831"
     CDS             complement(1361..2014)
                     /locus_tag="IWO31_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0303187.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          760 a          359 c          195 g          986 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc
//
LOCUS       DACECS010000152         2255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.159, whole genome shotgun sequence.
ACCESSION   DACECS010000152 DACECS010000000
VERSION     DACECS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.159"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(284..1624)
                     /gene="glnA"
                     /locus_tag="IWO31_002832"
     CDS             complement(284..1624)
                     /gene="glnA"
                     /locus_tag="IWO31_002832"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0303188.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1652..2044)
                     /locus_tag="IWO31_002833"
     CDS             complement(1652..2044)
                     /locus_tag="IWO31_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0303189.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          642 a          499 c          381 g          733 t
ORIGIN      
        1 cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt
       61 tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca
      121 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
      181 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt
      241 caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa agttctagat
      301 attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg
      361 cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac
      421 gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc
      481 tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat
      541 tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag
      601 atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta
      661 ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa
      721 cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt
      781 tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat
      841 catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat
      901 tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg
      961 ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt
     1021 taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc
     1081 ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg
     1141 caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta
     1201 atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc
     1261 gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat
     1321 ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga
     1381 agatcatcca agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa
     1441 atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc
     1501 tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa
     1561 atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta
     1621 ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt
     1681 gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa
     1741 tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct
     1801 gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt
     1861 taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc
     1921 gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag
     1981 tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc
     2041 ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat
     2101 tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa
     2161 aaaataagaa cacaaaaaag gtatctagtt gattttcgta ccagatacct tttatttttt
     2221 attatactgt actatccttt attagtgttt ttatt
//
LOCUS       DACECS010000153         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.160, whole genome shotgun sequence.
ACCESSION   DACECS010000153 DACECS010000000
VERSION     DACECS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.160"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IWO31_002834"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IWO31_002834"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0303190.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          589 a          479 c          404 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACECS010000154         2203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.161, whole genome shotgun sequence.
ACCESSION   DACECS010000154 DACECS010000000
VERSION     DACECS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.161"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWO31_002835"
     CDS             <1..605
                     /locus_tag="IWO31_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0303191.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IWO31_002836"
     CDS             739..2082
                     /locus_tag="IWO31_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0303192.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          877 a          269 c          394 g          663 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aat
//
LOCUS       DACECS010000155         2182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.162, whole genome shotgun sequence.
ACCESSION   DACECS010000155 DACECS010000000
VERSION     DACECS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.162"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            950..1423
                     /locus_tag="IWO31_002837"
     CDS             950..1423
                     /locus_tag="IWO31_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0303193.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IWO31_002838"
     CDS             1432..1659
                     /locus_tag="IWO31_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0303194.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          707 a          345 c          321 g          809 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tg
//
LOCUS       DACECS010000156         2103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.163, whole genome shotgun sequence.
ACCESSION   DACECS010000156 DACECS010000000
VERSION     DACECS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.163"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWO31_002839"
     CDS             169..1539
                     /locus_tag="IWO31_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ0303195.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWO31_002840"
     CDS             1560..1844
                     /locus_tag="IWO31_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303196.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cat
//
LOCUS       DACECS010000157         2021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.164, whole genome shotgun sequence.
ACCESSION   DACECS010000157 DACECS010000000
VERSION     DACECS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.164"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWO31_002841"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWO31_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0303197.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWO31_002842"
     CDS             852..1040
                     /locus_tag="IWO31_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0303198.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWO31_002843"
     CDS             1053..1610
                     /locus_tag="IWO31_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0303199.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWO31_002844"
     CDS             1642..1884
                     /locus_tag="IWO31_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0303200.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          743 a          309 c          407 g          562 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa a
//
LOCUS       DACECS010000158         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.165, whole genome shotgun sequence.
ACCESSION   DACECS010000158 DACECS010000000
VERSION     DACECS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.165"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWO31_002845"
     CDS             <1..378
                     /locus_tag="IWO31_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0303201.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWO31_002846"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWO31_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0303202.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWO31_002847"
     CDS             1859..>2003
                     /locus_tag="IWO31_002847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0303203.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACECS010000159         1932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.166, whole genome shotgun sequence.
ACCESSION   DACECS010000159 DACECS010000000
VERSION     DACECS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.166"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            202..1167
                     /locus_tag="IWO31_002848"
     CDS             202..1167
                     /locus_tag="IWO31_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0303204.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1367..1570
                     /locus_tag="IWO31_002849"
     CDS             1367..1570
                     /locus_tag="IWO31_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303205.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
BASE COUNT          640 a          288 c          436 g          568 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 ttctttgagg aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga gtgggaaaat
      241 ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc cctaaaatta
      301 cagtggtcaa ttgaccgaat tactatcgtt ggaaaactga aggaaaatat ttattatcac
      361 actcaaaatg atgtgttgat tttagatttt gagcaattaa tgcgggtgaa tgaaggcaat
      421 ggctatttaa aagcggttgg gaataacggc tggcaattat tagaccaata cgaagaaaat
      481 attgcttata ttgagatttt gaaatggcaa gatggcaaag ggcggattga ttttaacccg
      541 aataagattg gtcatttttt agctagttca atgaaaaatt tcattcatga tttatttcta
      601 gaaccacatt tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt
      661 attacacaat atcgagtggt tgatcctgtt tctttcaagc caatttatgg gcggtctggg
      721 aagctggaaa cagcttattg gggcagtcgt gccagcgaac gacaaattcg gctatacaat
      781 aaaaaattgg aacaagaaac caaacgaaaa gttgtaccaa aggaagttga gacttggtgg
      841 cggttagaaa tgcaactacg gcgtggcaag gcaaccgatt ggcataatat ggttcaagaa
      901 agtttgaata gttttgcgag cctgcatttt ttgccgattg atacaacacc tacagataga
      961 ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc gaggaattca
     1021 aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt gacgaatcat
     1081 ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga cacgtggctt
     1141 ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg agtgagagta
     1201 aatttacgtc aatgtttaat gacgtgagtt tacgataagc gattattttg cgtgaagcaa
     1261 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1321 ttgtgacagc gaataccctc ggagagcatt tggaattgtg acagccatgt cacaattagg
     1381 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1441 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaaaaatac
     1501 aagcatttct cataataatc gtgagttaac tgatagccaa aaaattaata gtttgtctgg
     1561 tagaaaataa ttagcagatt tacaagtggg aaataaaaag ctgaaagaag aaaattgcat
     1621 ttcaaactac attttctaca ataactctaa aatggcatat tttgcgttct gagcggtttt
     1681 agcgtttatt tcgtttagtt atcggcacaa tcggtgaaac acgttagagg agcgaaaatg
     1741 gagcctgtaa ggcgactggt agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa
     1801 cgaaataaat gctggcagcg ccattctatt tcggcaggag gaggctcaag gggttcgggg
     1861 aatgaaattc cctgaggtct ttttattttt tctagaggag tgctataatc aaagaaaatg
     1921 agaaaaaaga gt
//
LOCUS       DACECS010000160         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.167, whole genome shotgun sequence.
ACCESSION   DACECS010000160 DACECS010000000
VERSION     DACECS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.167"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWO31_002850"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWO31_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0303206.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACECS010000161         1774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.169, whole genome shotgun sequence.
ACCESSION   DACECS010000161 DACECS010000000
VERSION     DACECS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.169"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            390..>1774
                     /locus_tag="IWO31_002851"
     CDS             390..>1774
                     /locus_tag="IWO31_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0303207.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFT"
BASE COUNT          549 a          313 c          356 g          556 t
ORIGIN      
        1 caatttcaaa aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg
       61 gcaggagaac accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag
      121 gagctgcgac aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct
      181 tttgaatacc gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc
      241 gcttacatcg cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata
      301 aaaaaatctt aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga
      361 aaaaatgtga atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc
      421 gtttagcaat cgttttgaca gctgttttct ttttctggct gaagacgatc attgcctact
      481 atgcagattt ttcattaggt gtggaaggta cgatccagta ctttatcttg tggatcaacc
      541 caattgcgac taccttattg ttttttggcc tttctttgta tattaaaaag ccaaagccag
      601 cgttagctat tcttttgatc attgatattt taaatacgct tttgttgtac ttgaatatta
      661 ttttttatcg ggaattcaca gatttcatta cagtcaaatc tgtattaggc ttttcgaaag
      721 tgtcgcaagg attgtcagga agctcctttt ctttgatgaa gccccatgat gtcatctact
      781 ggctggatat cgctgtgttc attgggctac tggtttggtt gaaggtcaag aaaataccta
      841 tcaaaagcaa ccctgtcggc aaaccgatgg ctatcgctgt tacctgtctt tcagggctta
      901 ttttttcagg aaatctagcg ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat
      961 ttgaccgttc atatattgtc aaatatctag gaatcgatgc gtatactatt tatgacggaa
     1021 tcaaaacagg aatgactagc agtgttcgcg cgcatgcaag cagtaatggg attgatgaag
     1081 ttttagatta tacaaagaaa cattatgcag agccaaatcc cgaaacattt gggatcgcta
     1141 aagggaaaaa tgtgatcgtt ctccatttag aaagtttcca gcagtttttg attaatatga
     1201 aggtagacgg acaagaagtc acaccgtttt taaatagtat tttccagaat caagcaacga
     1261 tcagctttga caatttcttt catgaagtag gacagggaaa gacaagtgat gctgaaaata
     1321 tgttggaaac tggcacattt ggtttacctc aaggctcatt attcacagaa cttgggtcag
     1381 acaatgtctt tcaagccgca ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg
     1441 ttttccatgg gaatgtagct agtttttgga atagagatca tgtatataaa aacttaggtt
     1501 atgacaattt ctttgatcga tcttattttg atgaatccga cgaaacgtta ggttatggga
     1561 tattagataa agatttattc agagaatctg cccaatatct tgagcatcta caacagccgt
     1621 tttacacgaa atttctttcc gtaaccaacc acacgcctta ttatacggat gacaagaatt
     1681 tcgactttcc atctttgaat actggcaata gcacagtaga taattatgtt cgaacggcac
     1741 attatctgga tcaatcattg gaacaatttt ttac
//
LOCUS       DACECS010000162         1741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.170, whole genome shotgun sequence.
ACCESSION   DACECS010000162 DACECS010000000
VERSION     DACECS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.170"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..1228
                     /gene="aph(2'')-I"
                     /locus_tag="IWO31_002852"
                     /pseudo
     CDS             1..1228
                     /gene="aph(2'')-I"
                     /locus_tag="IWO31_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1212..1604
                     /locus_tag="IWO31_002853"
     CDS             1212..1604
                     /locus_tag="IWO31_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0303208.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1605..>1741
                     /locus_tag="IWO31_002854"
     CDS             1605..>1741
                     /locus_tag="IWO31_002854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HAQ0303209.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     S"
BASE COUNT          717 a          133 c          298 g          593 t
ORIGIN      
        1 aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa
       61 caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat atactgatta
      121 tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa
      181 ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa
      241 agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag
      301 ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga
      361 gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt
      421 taaggcaatg aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga
      481 aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa
      541 aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt
      601 tttaaataca aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag
      661 tgatgaatta tctatactag gttataaaga aattaaagga acttttttaa caccagaaat
      721 ttattctact atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt
      781 aagacaaatg cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca
      841 aaatgtatta gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat
      901 agaaaaagat tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg
      961 taaaaagtgt ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa
     1021 tagattaact ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt
     1081 tatatactta cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt
     1141 aagaatgtat ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga
     1201 atattatcct attgaaacta ttgtttattg atgataatat ggtttttgat attgataatt
     1261 taaaaggatt tcttaatgat accagttcat ttgggtttat agctaaagaa aataataaaa
     1321 ttataggatt tgcatattgc tatacacttt taagacctga tggaaaaaca atgttttatt
     1381 tacactcaat aggaatgtta cctaactatc aagacaaagg ttatggttca aaattattat
     1441 cttttattaa ggaatattct aaagagattg gttgttctga aatgttttta ataactgata
     1501 aaggtaatcc tagagcttgc catgtatatg aaaaattagg tggtaaaaat gattataaag
     1561 atgaaatagt atatgtatat gattatgaaa aaggtgataa ataaatgaat atagttgaaa
     1621 atgaaatatg tataagaact ttaatagatg atgattttcc tttgatgtta aaatggttaa
     1681 ctgatgaaag agtattagaa ttttatgatg gtagagataa aaaatataca ttagaatcat
     1741 t
//
LOCUS       DACECS010000163         1678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.171, whole genome shotgun sequence.
ACCESSION   DACECS010000163 DACECS010000000
VERSION     DACECS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.171"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IWO31_002855"
     CDS             316..570
                     /locus_tag="IWO31_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0303210.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IWO31_002856"
     CDS             571..888
                     /locus_tag="IWO31_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0303211.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1678)
                     /locus_tag="IWO31_002857"
     CDS             complement(1522..>1678)
                     /locus_tag="IWO31_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0303212.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          590 a          263 c          317 g          508 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaa
//
LOCUS       DACECS010000164         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.172, whole genome shotgun sequence.
ACCESSION   DACECS010000164 DACECS010000000
VERSION     DACECS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.172"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..1610
                     /locus_tag="IWO31_002858"
     CDS             63..1610
                     /locus_tag="IWO31_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0303213.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          544 a          290 c          333 g          444 t
ORIGIN      
        1 tcttgtgcgt ttcttgtggt attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa
       61 ttttggaaac tacagatacg ttgcttcagt tgcttcaaga agcccataaa accaatcaag
      121 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
      181 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      241 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      301 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      361 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      421 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      481 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      541 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      601 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      661 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      721 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      781 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      841 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      901 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      961 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
     1021 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
     1081 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1141 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1201 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1261 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1321 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1381 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1441 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1501 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1561 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa a
//
LOCUS       DACECS010000165         1531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.173, whole genome shotgun sequence.
ACCESSION   DACECS010000165 DACECS010000000
VERSION     DACECS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.173"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            90..941
                     /locus_tag="IWO31_002859"
     CDS             90..941
                     /locus_tag="IWO31_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303214.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            962..1174
                     /locus_tag="IWO31_002860"
     CDS             962..1174
                     /locus_tag="IWO31_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303215.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          570 a          201 c          310 g          450 t
ORIGIN      
        1 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
       61 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
      121 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
      181 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
      241 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
      301 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
      361 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
      421 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
      481 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
      541 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
      601 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
      661 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
      721 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
      781 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
      841 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
      901 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
      961 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     1021 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     1081 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     1141 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     1201 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     1261 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     1321 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     1381 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     1441 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     1501 cgtaagagaa attgatcgaa gatgtgacga a
//
LOCUS       DACECS010000166         1494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.174, whole genome shotgun sequence.
ACCESSION   DACECS010000166 DACECS010000000
VERSION     DACECS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.174"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWO31_002861"
     CDS             139..477
                     /locus_tag="IWO31_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303216.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWO31_002862"
     CDS             complement(682..1257)
                     /locus_tag="IWO31_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0303217.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          462 a          311 c          253 g          468 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgg
//
LOCUS       DACECS010000167         1469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.175, whole genome shotgun sequence.
ACCESSION   DACECS010000167 DACECS010000000
VERSION     DACECS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.175"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            288..695
                     /locus_tag="IWO31_002863"
     CDS             288..695
                     /locus_tag="IWO31_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303218.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IWO31_002864"
     CDS             709..1110
                     /locus_tag="IWO31_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303219.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..>1469
                     /locus_tag="IWO31_002865"
     CDS             1112..>1469
                     /locus_tag="IWO31_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303220.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT          562 a          178 c          258 g          471 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataa
//
LOCUS       DACECS010000168         1452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.176, whole genome shotgun sequence.
ACCESSION   DACECS010000168 DACECS010000000
VERSION     DACECS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.176"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWO31_002866"
     CDS             complement(<1..625)
                     /locus_tag="IWO31_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0303221.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWO31_002867"
     CDS             complement(784..1050)
                     /locus_tag="IWO31_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0303222.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWO31_002868"
     CDS             complement(1040..1396)
                     /locus_tag="IWO31_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0303223.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aa
//
LOCUS       DACECS010000169         1427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.177, whole genome shotgun sequence.
ACCESSION   DACECS010000169 DACECS010000000
VERSION     DACECS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.177"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWO31_002869"
     CDS             105..1406
                     /locus_tag="IWO31_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0303224.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          471 a          287 c          262 g          407 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctacct
//
LOCUS       DACECS010000170         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.178, whole genome shotgun sequence.
ACCESSION   DACECS010000170 DACECS010000000
VERSION     DACECS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.178"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..232
                     /locus_tag="IWO31_002870"
     CDS             149..232
                     /locus_tag="IWO31_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0303225.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            357..1094
                     /gene="erm(B)"
                     /locus_tag="IWO31_002871"
     CDS             357..1094
                     /gene="erm(B)"
                     /locus_tag="IWO31_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0303226.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          515 a          221 c          237 g          445 t
ORIGIN      
        1 tcttgcgtat ggttaaccct aaagttatgg aaataagact tagaagcaaa cttaagagtg
       61 tgttgatagt gcattaaaaa ttttgtataa taggaattga agttaaatta gatgctaaaa
      121 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgtt atgtagataa
      181 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
      241 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
      301 caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggagt gattacatga
      361 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
      421 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
      481 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
      541 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
      601 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc
      661 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca
      721 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
      781 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
      841 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
      901 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
      961 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     1021 atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta
     1081 acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa
     1141 gggaatgtag ataaattatt aggtataggg cacctctaat aactaccaat taattcacct
     1201 caaaatgaaa ttagaaaaaa acagcgaaat ttttaaatct atttcttatc gatacaaatt
     1261 cctcgtaggc gctcgggacc ccaacatgag gaaatgtttt tataaaaaaa gagtagttac
     1321 cgttttttgg taactactct tttccttttt taaggtttaa atacctggaa gtttaggtgt
     1381 tttgggtatt ggtggtgtag gtggctgcaa gctttcca
//
LOCUS       DACECS010000171         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.181, whole genome shotgun sequence.
ACCESSION   DACECS010000171 DACECS010000000
VERSION     DACECS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.181"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWO31_002872"
     rRNA            <1..>1355
                     /locus_tag="IWO31_002872"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACECS010000172         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.182, whole genome shotgun sequence.
ACCESSION   DACECS010000172 DACECS010000000
VERSION     DACECS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.182"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWO31_002873"
     CDS             <1..848
                     /locus_tag="IWO31_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0303227.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACECS010000173         1310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.183, whole genome shotgun sequence.
ACCESSION   DACECS010000173 DACECS010000000
VERSION     DACECS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.183"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..>1310)
                     /locus_tag="IWO31_002874"
     CDS             complement(150..>1310)
                     /locus_tag="IWO31_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0303228.1"
                     /translation="SEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQYIQANAYER
                     SEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKALIAAMIEMV
                     ISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYPFFMCDA
                     LYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          380 a          290 c          232 g          408 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct
//
LOCUS       DACECS010000174         1303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.184, whole genome shotgun sequence.
ACCESSION   DACECS010000174 DACECS010000000
VERSION     DACECS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.184"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1253)
                     /locus_tag="IWO31_002875"
     rRNA            complement(<1..1253)
                     /locus_tag="IWO31_002875"
                     /product="16S ribosomal RNA"
BASE COUNT          282 a          391 c          291 g          339 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgttcg
     1141 ccactcctct ttttccggtg gagcaagctc cggtggaaaa agaagcgttc gacttgcatg
     1201 tattaggcac gccgccagcg ttcgtcctga gccaggatca aactctcata aaaagttcga
     1261 acaatcttgc gattgttaag ctcaatttgt ttgctagcat att
//
LOCUS       DACECS010000175         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.185, whole genome shotgun sequence.
ACCESSION   DACECS010000175 DACECS010000000
VERSION     DACECS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.185"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO31_002876"
     CDS             141..398
                     /locus_tag="IWO31_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303229.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO31_002877"
     CDS             complement(630..1043)
                     /locus_tag="IWO31_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0303230.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaa
//
LOCUS       DACECS010000176         1255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.186, whole genome shotgun sequence.
ACCESSION   DACECS010000176 DACECS010000000
VERSION     DACECS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.186"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..1089
                     /locus_tag="IWO31_002878"
     CDS             10..1089
                     /locus_tag="IWO31_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0303231.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          371 a          300 c          307 g          277 t
ORIGIN      
        1 tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa acatctttcc
       61 tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa ccgtcaggtt
      121 gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg cggaaccatc
      181 acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta ttctgtttat
      241 gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg cagacgcccc
      301 aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt aaatgaaaaa
      361 tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga ttgtgccatc
      421 attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg aacaaagaac
      481 atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg acaccgtccc
      541 aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga aacacgggaa
      601 acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc ggatgccgtg
      661 ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt gaacgggaag
      721 gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg cgctaacttc
      781 gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg gctacacgat
      841 gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg ggagcgtggg
      901 acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag cccgattagt
      961 gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta cccgcggaaa
     1021 attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca agaacggttg
     1081 gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct tcatgtctgg
     1141 aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca ttatcaaggc
     1201 ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta cctgt
//
LOCUS       DACECS010000177         1251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.187, whole genome shotgun sequence.
ACCESSION   DACECS010000177 DACECS010000000
VERSION     DACECS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.187"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..1089
                     /locus_tag="IWO31_002879"
     CDS             10..1089
                     /locus_tag="IWO31_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0303232.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          328 a          341 c          353 g          229 t
ORIGIN      
        1 tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc
       61 tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt
      121 gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt
      181 actcagctta accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac
      241 gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc
      301 ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa
      361 tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt
      421 atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat
      481 atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg
      541 aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa
      601 accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg
      661 ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag
      721 gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc
      781 ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat
      841 gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg
      901 acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc
      961 aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag
     1021 attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg
     1081 acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa
     1141 gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac
     1201 tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DACECS010000178         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.188, whole genome shotgun sequence.
ACCESSION   DACECS010000178 DACECS010000000
VERSION     DACECS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.188"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWO31_002880"
     CDS             200..742
                     /locus_tag="IWO31_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303233.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWO31_002881"
     CDS             832..>1246
                     /locus_tag="IWO31_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0303234.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACECS010000179         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.189, whole genome shotgun sequence.
ACCESSION   DACECS010000179 DACECS010000000
VERSION     DACECS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.189"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO31_002882"
     CDS             414..947
                     /locus_tag="IWO31_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0303235.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACECS010000180         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.190, whole genome shotgun sequence.
ACCESSION   DACECS010000180 DACECS010000000
VERSION     DACECS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.190"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWO31_002883"
     CDS             <1..1076
                     /locus_tag="IWO31_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0303236.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACECS010000181         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.191, whole genome shotgun sequence.
ACCESSION   DACECS010000181 DACECS010000000
VERSION     DACECS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.191"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWO31_002884"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWO31_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0303237.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACECS010000182         1066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.192, whole genome shotgun sequence.
ACCESSION   DACECS010000182 DACECS010000000
VERSION     DACECS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.192"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..782
                     /locus_tag="IWO31_002885"
                     /pseudo
     CDS             81..782
                     /locus_tag="IWO31_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          353 a          176 c          181 g          356 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacaat tattccgtta tatttatgga tgcgacgcat atccctgtga
      121 agcgacagac tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa
      181 ccaaagaagt tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag
      241 ttcttcaaga tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat
      301 taagctgtat tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt
      361 gtgtgcatgt atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt
      421 gtgaggattt caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg
      481 actttatgac taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc
      541 ctgccctatt aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga
      601 acctgattga aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc
      661 ctaatgaaga atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat
      721 ttttaggtag aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt
      781 aactgtaatt aacagaatgg attttccatt tacatataat tcttgacgct accataatct
      841 aaataaccat catattcatc tactacttat ataattgagt tttattaaac tttttaagtt
      901 tctattctat caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt
      961 actaacaata tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt
     1021 tcactttcaa taagaaaaaa gtctttactt ccatggataa taataa
//
LOCUS       DACECS010000183         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.193, whole genome shotgun sequence.
ACCESSION   DACECS010000183 DACECS010000000
VERSION     DACECS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.193"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO31_002886"
     CDS             complement(36..992)
                     /locus_tag="IWO31_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303238.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACECS010000184         1046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.195, whole genome shotgun sequence.
ACCESSION   DACECS010000184 DACECS010000000
VERSION     DACECS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.195"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWO31_002887"
     CDS             6..980
                     /locus_tag="IWO31_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0303239.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaaca
//
LOCUS       DACECS010000185         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.196, whole genome shotgun sequence.
ACCESSION   DACECS010000185 DACECS010000000
VERSION     DACECS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.196"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1045
                     /locus_tag="IWO31_002888"
     CDS             <1..>1045
                     /locus_tag="IWO31_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0303240.1"
                     /translation="WSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGF
                     DAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLY
                     SEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNR
                     AYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIE
                     FYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKEVYRQILNA
                     FDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPN
                     MKAIINSKDYEGY"
BASE COUNT          373 a          165 c          216 g          291 t
ORIGIN      
        1 cctggtcact agcagacatt gagcgttata acggagacat caataatcgt ttggcacgaa
       61 aaagagcgga attcaaacaa tttgggaaac aaatcgattt agtaatcgta gtggatcgtc
      121 tattgacagg ttttgacgcc cctacgattc aaaccctatt tgtggatcgt aatctagagt
      181 atgcaggatt gatccaagcc ttttcacgta cgaaccgtac gtatcctgaa aaaacaaaag
      241 gactaattgt tactttccga aagccagcga caatggagaa gaatgtcgag gatgcgacaa
      301 aactgtattc agaagctaaa gaagaatcgg gtttgattta tccaagttac caagaatcca
      361 aaaaaagatt caagcaagca tatggtaagt taaaagaggt ttcatccatg gaatccattg
      421 atgaacatac gccacttgaa atccgtatcg agtacgtcaa agcatttcaa gaattgaatc
      481 gtgcgtatga atcgttagtg acttatgatg attacaatga tgacatggtg caatcttcaa
      541 ctttgaacaa tcaaattcaa cttttggaag aacaaattgg cgtgtacgag accgtgaagg
      601 gttcattgat ggaggaagaa ccagaaaaag agggagaaga tttttcaacg atcgaatttt
      661 atagtgaaaa taccactaag ttgtatgata ttgattctac ttatattgat caattactag
      721 gaacttatgc tgcgaatagt tcagatgcac gagaagaaat tgaaaaagct ttggctaaat
      781 tgaataaaac agaaggagtc aaagaagttt accgacaaat tttaaatgca tttgatgtgg
      841 ggactttaga tagtaatgaa gatatctttg tcattaaacg tcgctatttt acacaagcaa
      901 gtgataattt gattcatgcc ttttccaatg agtggtttgt ttctgagaaa gaattacatg
      961 cttctaatct gcaatatatg ccaggtgaag atccaattcc taatatgaaa gcaattatca
     1021 acagtaagga ttacgaagga tataa
//
LOCUS       DACECS010000186          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.197, whole genome shotgun sequence.
ACCESSION   DACECS010000186 DACECS010000000
VERSION     DACECS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.197"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..912)
                     /locus_tag="IWO31_002889"
     CDS             complement(25..912)
                     /locus_tag="IWO31_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0303241.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DACECS010000187          986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.198, whole genome shotgun sequence.
ACCESSION   DACECS010000187 DACECS010000000
VERSION     DACECS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.198"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>986
                     /locus_tag="IWO31_002890"
     CDS             <1..>986
                     /locus_tag="IWO31_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0303242.1"
                     /translation="KCKSCQRTFVEDTSVAEKHCFISQKVRWSVIARLKENTSMTEIA
                     RQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          305 a          199 c          191 g          291 t
ORIGIN      
        1 ttaaatgtaa gtcttgccaa agaacattcg tagaggatac ctctgttgct gaaaaacact
       61 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      121 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      181 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      241 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      301 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      361 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      421 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      481 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      541 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      601 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
      661 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
      721 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
      781 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
      841 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
      901 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
      961 gctttcgcaa cttttacaat ttcaaa
//
LOCUS       DACECS010000188          957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.199, whole genome shotgun sequence.
ACCESSION   DACECS010000188 DACECS010000000
VERSION     DACECS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.199"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(285..506)
                     /locus_tag="IWO31_002891"
     CDS             complement(285..506)
                     /locus_tag="IWO31_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303243.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(604..849)
                     /locus_tag="IWO31_002892"
     CDS             complement(604..849)
                     /locus_tag="IWO31_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303244.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          289 a          183 c          119 g          366 t
ORIGIN      
        1 taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata cttcttccca
       61 gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt ggtttgccca
      121 tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat ttcaattata
      181 tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa gctgtatttt
      241 acatatagta accaaagagt taataaaata aaaagagcga accattatag atcagctctt
      301 ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa ggtatttaca
      361 tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc aatattacgg
      421 taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa ttcccagcgt
      481 ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac ctcaaactta
      541 aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa atatgaaaaa
      601 aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct taatgtgttt
      661 cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg ttgtgtattt
      721 tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat agttcatagg
      781 agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta caacttctgg
      841 aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt tatctttcct
      901 tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta tttaact
//
LOCUS       DACECS010000189          936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.200, whole genome shotgun sequence.
ACCESSION   DACECS010000189 DACECS010000000
VERSION     DACECS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.200"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..>936
                     /locus_tag="IWO31_002893"
     rRNA            47..>936
                     /locus_tag="IWO31_002893"
                     /product="23S ribosomal RNA"
BASE COUNT          288 a          185 c          273 g          190 t
ORIGIN      
        1 ctaacttcta tcgctagaag aaggatcaaa acccaaccgt aaggttgata aggttaagtg
       61 aataagggcg cacggtggat gccttggcac taggagccga tgaaggacgg gactaacacc
      121 gatatgcttt ggggagctgt acgtaagcta tgatccagag atttccgaat gggggaaccc
      181 aacatcttta ataggatgtt acgattgtgt gaatacatag cacattcgag gtagacgcag
      241 agaactgaaa catctaagta cctgcaggaa gagaaagaaa attcgattcc ctgagtagcg
      301 gcgagcgaaa cgggaaaagc ccaaaccagc aagcttgctt gttggggttg taggactcca
      361 atatggtagt tctttcagat agtcgaatga cttggaaaag tcagtcaaag agggtaaaaa
      421 ccccgtagac gaaatgtgga agacacctag gaggatcctg agtacggcgg aacacgagaa
      481 attccgtcgg aatccgggag gaccatctcc caaggctaaa tactccctag tgaccgatag
      541 tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga aggggagtga aatagaacct
      601 gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg tgatggcgtg ccttttgtag
      661 aatgaaccgg cgagttacga ttgcatgcga ggttaagttg aagagacgga gccgcagcga
      721 aagcgagtct gaatagggcg tttgagtatg tagtcgtaga cccgaaacca tgtgatctac
      781 ccatgtccag gttgaaggtg cggtaaaacg cactggagga ccgaacccac gtacgttgaa
      841 aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt
      901 tctctccgaa atagctttag ggctagcctc ggaatt
//
LOCUS       DACECS010000190          916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.201, whole genome shotgun sequence.
ACCESSION   DACECS010000190 DACECS010000000
VERSION     DACECS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.201"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IWO31_002894"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IWO31_002894"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IWO31_002895"
     tRNA            203..276
                     /locus_tag="IWO31_002895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IWO31_002896"
     tRNA            316..405
                     /locus_tag="IWO31_002896"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IWO31_002897"
     tRNA            420..491
                     /locus_tag="IWO31_002897"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IWO31_002898"
     tRNA            507..579
                     /locus_tag="IWO31_002898"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IWO31_002899"
     tRNA            589..661
                     /locus_tag="IWO31_002899"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IWO31_002900"
     tRNA            697..772
                     /locus_tag="IWO31_002900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IWO31_002901"
     tRNA            774..854
                     /locus_tag="IWO31_002901"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
BASE COUNT          227 a          199 c          245 g          245 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaa
//
LOCUS       DACECS010000191          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.202, whole genome shotgun sequence.
ACCESSION   DACECS010000191 DACECS010000000
VERSION     DACECS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.202"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(57..>863)
                     /locus_tag="IWO31_002902"
     CDS             complement(57..>863)
                     /locus_tag="IWO31_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ0303245.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          231 a          205 c          162 g          265 t
ORIGIN      
        1 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
       61 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
      121 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
      181 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
      241 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
      301 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
      361 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
      421 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
      481 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
      541 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
      601 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
      661 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
      721 cgactgagtt gaagcccacg ctttgctaaa cacttcttca attttcgggt accgtaagcc
      781 tttcggtttc gaataaactc ttcctgaacg atctcttcta agtcggactc attttcgatc
      841 ggtttcgctt gataatagta ggt
//
LOCUS       DACECS010000192          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.203, whole genome shotgun sequence.
ACCESSION   DACECS010000192 DACECS010000000
VERSION     DACECS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.203"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..101)
                     /locus_tag="IWO31_002903"
     tRNA            complement(14..101)
                     /locus_tag="IWO31_002903"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Ser,seq:gct)
     gene            complement(126..199)
                     /locus_tag="IWO31_002904"
     tRNA            complement(126..199)
                     /locus_tag="IWO31_002904"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Ile,seq:gat)
     gene            complement(213..283)
                     /locus_tag="IWO31_002905"
     tRNA            complement(213..283)
                     /locus_tag="IWO31_002905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Gly,seq:tcc)
     gene            complement(285..360)
                     /locus_tag="IWO31_002906"
     tRNA            complement(285..360)
                     /locus_tag="IWO31_002906"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Asp,seq:gtc)
     gene            complement(379..452)
                     /locus_tag="IWO31_002907"
     tRNA            complement(379..452)
                     /locus_tag="IWO31_002907"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(416..418),aa:Met,seq:cat)
     gene            complement(465..554)
                     /locus_tag="IWO31_002908"
     tRNA            complement(465..554)
                     /locus_tag="IWO31_002908"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Ser,seq:tga)
     gene            complement(578..651)
                     /locus_tag="IWO31_002909"
     tRNA            complement(578..651)
                     /locus_tag="IWO31_002909"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ile,seq:cat)
     gene            complement(665..738)
                     /locus_tag="IWO31_002910"
     tRNA            complement(665..738)
                     /locus_tag="IWO31_002910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(702..704),aa:Met,seq:cat)
     gene            complement(766..838)
                     /locus_tag="IWO31_002911"
     tRNA            complement(766..838)
                     /locus_tag="IWO31_002911"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(803..805),aa:Ala,seq:tgc)
BASE COUNT          230 a          237 c          205 g          183 t
ORIGIN      
        1 caaaatattg aaacggagaa ggagggattt gaaccctcgc gccggtttcc cgacctacac
       61 ccttagcagg ggcgcctctt cagccacttg agtacttccc caaaaccaat aagattttgt
      121 actaatgggc ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa
      181 ccagctgagc tataggccca taaaaaacta aaagcgggtg acgagaatcg aactcgcgac
      241 aacagcttgg aaggctgtgg ttttaccact aaactacacc cgcatggcgg ttccgacggg
      301 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
      361 agtgttttta gtttttaatt gcgggagcag gatttgaacc tacgaccttc gggttatgag
      421 cccgacgagc taccagactg ctccatcccg cgataatatg aaaaaggagg ataagggatt
      481 cgaacccttg cacggtttta cccgcctgac ggttttcaag accgttccct tcagccggac
      541 ttgggtaatc ctccatatga aacttacaac gccacaatgg accttgtagg actcgaacct
      601 acgaccggac ggttatgagc cgtctgctct aaccaactga gctaaaggtc caggctctta
      661 aaaatcgcgg cggaggggat cgaacccccg acctcccggg tatgaaccgg acgctctagc
      721 cagctgagct acaccgcgaa aaagatattc attatttaaa tgaaatggag cctagcggga
      781 tcgaaccgct gacctcctgc gtgcaaagca ggcgctctcc cagctgagct aaggccccgt
      841 gtttaaattt ataat
//
LOCUS       DACECS010000193          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.204, whole genome shotgun sequence.
ACCESSION   DACECS010000193 DACECS010000000
VERSION     DACECS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.204"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            15..>846
                     /locus_tag="IWO31_002912"
     CDS             15..>846
                     /locus_tag="IWO31_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0303246.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          290 a          147 c          164 g          245 t
ORIGIN      
        1 aggagtgagt acaaatgacc tatacccatc ttacaacgga tgaacttgta ataatagagt
       61 cttatttcaa aatgaatcaa tctgttgcta aaactgccca ttgcttgaat cgttcaagac
      121 aaacgatcca taaagtatat ctgttcttca agcaaggaaa atcagcctta gaatattatc
      181 aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc gcttgtttta cccgaggaac
      241 aatcagaata tattcaaaga aaggttgttc aaggatggac acccgatgtg attgttggtc
      301 gtgcagcgtt tcctattagt tgttctgctc gtaccattta tcgtatgttc aaaaaggggc
      361 tatttgattc ttctgactta ccgatgaaag gcaagcgtaa accgaatgga catcaagaaa
      421 gacgtggaaa acaaactttc cgccgctcta ttcatgaacg tgaaaaggat tatagccaat
      481 tctcaaatga gtttggtcac cttgaaggtg acactatcgt aggtctgaaa cataaaagtg
      541 ctgtaattac cttagttgaa cgattatcaa aagttatcat cacattgaaa ccgtgtggta
      601 gacaagcgat tgatattgaa aaaaaattaa atcaatggtt tgaatctgta ccgaaaaacc
      661 tattcaaatc catcactttt gattgtggaa aggaattttc aaattggaaa cagatcagta
      721 atgtcaatga tattgccatt tatttcgctg atccaggaac gccgtctcaa agaggcctaa
      781 acgagaattc taacggattg ttacgtagag atggtttatt gaaatctatg gatttcaatt
      841 cagtag
//
LOCUS       DACECS010000194          816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.205, whole genome shotgun sequence.
ACCESSION   DACECS010000194 DACECS010000000
VERSION     DACECS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.205"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IWO31_002913"
     CDS             367..555
                     /locus_tag="IWO31_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303247.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IWO31_002914"
     CDS             545..805
                     /locus_tag="IWO31_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303248.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
BASE COUNT          253 a          125 c          183 g          255 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctagg
//
LOCUS       DACECS010000195          805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.206, whole genome shotgun sequence.
ACCESSION   DACECS010000195 DACECS010000000
VERSION     DACECS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.206"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWO31_002915"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWO31_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0303249.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          297 a          126 c          150 g          232 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtca
//
LOCUS       DACECS010000196          768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.207, whole genome shotgun sequence.
ACCESSION   DACECS010000196 DACECS010000000
VERSION     DACECS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.207"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            55..735
                     /locus_tag="IWO31_002916"
     CDS             55..735
                     /locus_tag="IWO31_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISEnfa1 family transposase"
                     /protein_id="HAQ0303250.1"
                     /translation="MTQFKGKQFQKDVIIVAVGYYLRYNLSYREVQEILYDRGIHVCH
                     TTIYRWVQEYGKILYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDVDGLTLDI
                     WLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKLQKNGLYITTEHRTIK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRGIYKKSRKEGSLFGFSVCTEI
                     KGLLGIPA"
BASE COUNT          284 a          133 c          153 g          198 t
ORIGIN      
        1 gataagctca ttttgaggta aaataattga aaaagatagc ttggaggaat gaagatgact
       61 cagttcaaag gaaaacaatt tcaaaaagac gtgattatcg tagccgtcgg ctactatctt
      121 cggtacaacc tgagttatcg tgaagtgcag gaaattctgt atgaccgagg aattcacgtt
      181 tgtcacacca caatttaccg ttgggtgcaa gaatacggaa agattcttta tcaaatttgg
      241 aaaaagaaaa acaaacagtc cttttattca tggaaaatgg acgagactta tatcaaaatt
      301 aaaggaaagt ggcattattt atatcgcgct atcgatgtag atggcttaac cttagatatc
      361 tggttacgga aaaagcgcga cacacaggct gcgtatgctt ttctaaaacg actgaagaac
      421 cagtttggag aaccaaaggt tctagtaacg gataaagcac cctctattaa gagtgccttc
      481 agaaagcttc agaaaaatgg actgtatata acaacagaac atcgaacaat caagtatctg
      541 aataatctga ttgaacaaga ccatcgtcca attaagcgaa gaaacaaatt ttaccaaagt
      601 ttgcgaacag cctcaaccac gattaaaggc atggaagcga ttcgaggaat ttataaaaaa
      661 agccgaaaag aagggtctct ttttggcttt tccgtctgta cagaaatcaa aggactatta
      721 ggaatccctg cttaaataag aatcatcttg aaaaactaat gacctttt
//
LOCUS       DACECS010000197          756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.208, whole genome shotgun sequence.
ACCESSION   DACECS010000197 DACECS010000000
VERSION     DACECS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.208"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            194..424
                     /locus_tag="IWO31_002917"
     CDS             194..424
                     /locus_tag="IWO31_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303251.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            430..729
                     /locus_tag="IWO31_002918"
     CDS             430..729
                     /locus_tag="IWO31_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303252.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          289 a          117 c          114 g          236 t
ORIGIN      
        1 acaaaaccac tatattctat tatgtatcta ataccctcaa aacagctata aatcaacaat
       61 accgacatat aataaacact atgttcaatc tgagatatat caaagataaa cgtatttttg
      121 cccccttttt gcccctcaaa tatcgcttta caacgagaac gtaagttcgt ataatcgttt
      181 tgaggtgatt tttatgatgg aagactttat tagaaaaaat atcagtgatg aatatgcaga
      241 cttttatgag caaagcagca aaaaagataa attccagatg gatgtttcaa ttttagctat
      301 attagctttt tccgaaaata aacaacctgt aactgcaaaa aaagaaacag tattctctga
      361 aggcaaaata aaaactcgat atatattaga ggtagaaact aattttaaaa acagatcgga
      421 gtaatggtta tgctttttaa tgagacacaa ttatggttta aattcgatcc tacgaataga
      481 tttatcaaag atttttataa agtatggaat tcagaagttt tctttttagc aatcgaagat
      541 agcttattaa tcaatctcta ctattctaat aagaactatt tcaaaattcc tgctgcgaaa
      601 actagaatga agaagggtgt atactttttg tttgatatcg tgactgacgt gccggacgca
      661 cgtagcgatc atcggcgtta tgactatata aagtatactt tcgttgatcc agaaagatac
      721 aaagattaaa taaaaaaata acaaacaaac tcgctt
//
LOCUS       DACECS010000198          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.209, whole genome shotgun sequence.
ACCESSION   DACECS010000198 DACECS010000000
VERSION     DACECS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.209"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWO31_002919"
     CDS             complement(<1..446)
                     /locus_tag="IWO31_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0303253.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACECS010000199          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.210, whole genome shotgun sequence.
ACCESSION   DACECS010000199 DACECS010000000
VERSION     DACECS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.210"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>697
                     /locus_tag="IWO31_002920"
     CDS             <1..>697
                     /locus_tag="IWO31_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ0303254.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQA"
BASE COUNT          219 a          136 c          136 g          206 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctt
//
LOCUS       DACECS010000200          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.211, whole genome shotgun sequence.
ACCESSION   DACECS010000200 DACECS010000000
VERSION     DACECS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.211"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..628)
                     /locus_tag="IWO31_002921"
     CDS             complement(53..628)
                     /locus_tag="IWO31_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0303255.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DACECS010000201          682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.212, whole genome shotgun sequence.
ACCESSION   DACECS010000201 DACECS010000000
VERSION     DACECS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.212"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWO31_002922"
     CDS             complement(<1..276)
                     /locus_tag="IWO31_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0303256.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          226 a          113 c          112 g          231 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa ac
//
LOCUS       DACECS010000202          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.213, whole genome shotgun sequence.
ACCESSION   DACECS010000202 DACECS010000000
VERSION     DACECS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.213"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IWO31_002923"
     CDS             complement(<1..270)
                     /locus_tag="IWO31_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303257.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="IWO31_002924"
     CDS             complement(276..>635)
                     /locus_tag="IWO31_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0303258.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DACECS010000203          623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.214, whole genome shotgun sequence.
ACCESSION   DACECS010000203 DACECS010000000
VERSION     DACECS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.214"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>623
                     /locus_tag="IWO31_002925"
     CDS             <1..>623
                     /locus_tag="IWO31_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0303259.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAF"
BASE COUNT          213 a          120 c          105 g          185 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttt
//
LOCUS       DACECS010000204          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.215, whole genome shotgun sequence.
ACCESSION   DACECS010000204 DACECS010000000
VERSION     DACECS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.215"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>610
                     /locus_tag="IWO31_002926"
     CDS             51..>610
                     /locus_tag="IWO31_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0303260.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTAS"
BASE COUNT          221 a          100 c          120 g          169 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc
//
LOCUS       DACECS010000205          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.216, whole genome shotgun sequence.
ACCESSION   DACECS010000205 DACECS010000000
VERSION     DACECS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.216"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..563)
                     /locus_tag="IWO31_002927"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="IWO31_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          168 a          109 c           78 g          240 t
ORIGIN      
        1 taattgtgca attgaaagta atagattcaa tttaaattct tttttgagct gaaggattgt
       61 tttaaccttg atttcttctt gctcaaatct tgttcttgaa tcaaggtttc taactttttt
      121 aaatatgcgt tctctgctct taaacgaata acttcttctt caagagactc atctttaagt
      181 tttttaggaa tgtttagctt ggaattcata ctaatttttc tgcccctttt ttggctctca
      241 agtgaagaag caccgccttc ttcatattgc tctatccatt tacttagagt tctatttgaa
      301 ccgatattaa attttttagc agtttcttgg atagaaagac catttgtttc catatattct
      361 ataacatcaa gtttaaattt tgtagtgtag cttttgccac ctccaaccaa gccttcccaa
      421 ccatgatagt tataaatcct tacccaatgt ctaaccagtg tacgatttat ttgatattta
      481 tgtgcaagat atttgtagcc gccttcgtta tttaaatagt ctgaaactac ttttttctta
      541 aaaacaaatg tatatttccg caaaaaaagc acccctttta attagatttc tagtc
//
LOCUS       DACECS010000206          535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.217, whole genome shotgun sequence.
ACCESSION   DACECS010000206 DACECS010000000
VERSION     DACECS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.217"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>535
                     /locus_tag="IWO31_002928"
     CDS             <1..>535
                     /locus_tag="IWO31_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303261.1"
                     /translation="SQHIGRAFTNHRIQVMKTFKKGDRRSKHLKKYWRLLQKNAWELN
                     GQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVFLSAIRRQDVPEFVALLKE
                     DYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQN
                     YRERIFLYRGKYFKKTK"
BASE COUNT          181 a          115 c          108 g          131 t
ORIGIN      
        1 ttagccaaca catcggtcgg gcctttacca accaccgaat tcaagtaatg aagaccttta
       61 aaaaagggga tcgtcggtcg aagcacttaa agaaatactg gagactactt caaaagaatg
      121 cgtgggaatt gaatggtcag catcgctact ggcgtccgtc atttcgagat catctgacag
      181 aagcggagat agtcgatcgg ctattatatt atgatgactc cttaaagcga ggctacgaag
      241 tctaccaggt ttttctttcc gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc
      301 taaaagaaga ctacaaggag ttgccagagc actatcaacc agtgttcacg accttcaaga
      361 aatatcgaac agaaatcaaa cgagcgttgc gagttcccta ttccaacggc cctatagaat
      421 gtttgaacaa ccatatcaaa gtactaaaac gaattgccta tggctttaga aactttcaaa
      481 actaccgaga acgaatcttt ttatatcgag gaaaatattt caagaaaact aagaa
//
LOCUS       DACECS010000207          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.218, whole genome shotgun sequence.
ACCESSION   DACECS010000207 DACECS010000000
VERSION     DACECS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.218"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWO31_002929"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWO31_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0303262.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACECS010000208          516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.219, whole genome shotgun sequence.
ACCESSION   DACECS010000208 DACECS010000000
VERSION     DACECS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.219"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..>516)
                     /locus_tag="IWO31_002930"
     CDS             complement(107..>516)
                     /locus_tag="IWO31_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0303263.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          172 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccat
//
LOCUS       DACECS010000209          511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.220, whole genome shotgun sequence.
ACCESSION   DACECS010000209 DACECS010000000
VERSION     DACECS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.220"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>511)
                     /locus_tag="IWO31_002931"
     CDS             complement(<1..>511)
                     /locus_tag="IWO31_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAQ0303264.1"
                     /translation="KNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDT
                     FKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPI
                     QTATMLQAKDYETKMYVMAVPKINSYLGTIERYETMYADDPMTARATPKQAHDIVVKN
                     LPTNLETLHK"
BASE COUNT          126 a           96 c           96 g          193 t
ORIGIN      
        1 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
       61 ttggtgttgc cctggctgtc attggatcat ctgcatacat agtttcatac cgttcaattg
      121 ttcctaaata tgagttgatt ttaggtactg ccatgacata catttttgtt tcataatctt
      181 tggcttgaag cattgttgcg gtttgaatag gaacgtctgt tgttcgtcct gtaccttcaa
      241 tcactaaatt atacccttta tctctcaaac ggcttatgat cgcttctgtc atgcgattag
      301 aataaggggt aacgtatttt actacgtctt tttcataaag tttcactagt tcatcaaaat
      361 tagggtgctg ttgtttgaac gtatcattat caatgataac aacatttcct tgtgtctctt
      421 cggaaattgc cgatcgcaaa ctagttttac ctgatcctgg ttgcccacca agtaaaaaag
      481 cggttggcga ttcaaccgct agtctatttt t
//
LOCUS       DACECS010000210          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.221, whole genome shotgun sequence.
ACCESSION   DACECS010000210 DACECS010000000
VERSION     DACECS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.221"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACECS010000211          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.222, whole genome shotgun sequence.
ACCESSION   DACECS010000211 DACECS010000000
VERSION     DACECS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.222"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IWO31_002932"
     CDS             complement(61..258)
                     /locus_tag="IWO31_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0303265.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACECS010000212          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.223, whole genome shotgun sequence.
ACCESSION   DACECS010000212 DACECS010000000
VERSION     DACECS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.223"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>468
                     /locus_tag="IWO31_002933"
     CDS             <1..>468
                     /locus_tag="IWO31_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303266.1"
                     /translation="FHEDAFRKERINDETCFVFDGTLTYQPEECFHCHYQNKQTIIKW
                     GWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAEDSVSDRRCSIARRVKQAILE
                     LLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQPLPEVVCFDEFKSVK"
BASE COUNT          142 a          100 c           92 g          134 t
ORIGIN      
        1 ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt ttgtttttga
       61 tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa ataagcaaac
      121 gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa
      181 gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt
      241 agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca aacaagccat
      301 tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga agcacatttc
      361 tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt ctttgaacca
      421 gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaa
//
LOCUS       DACECS010000213          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.224, whole genome shotgun sequence.
ACCESSION   DACECS010000213 DACECS010000000
VERSION     DACECS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.224"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..462
                     /locus_tag="IWO31_002934"
                     /pseudo
     CDS             1..462
                     /locus_tag="IWO31_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          191 a           79 c           85 g          107 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc ca
//
LOCUS       DACECS010000214          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.225, whole genome shotgun sequence.
ACCESSION   DACECS010000214 DACECS010000000
VERSION     DACECS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.225"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IWO31_002935"
     CDS             <1..>452
                     /locus_tag="IWO31_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303267.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DACECS010000215          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.226, whole genome shotgun sequence.
ACCESSION   DACECS010000215 DACECS010000000
VERSION     DACECS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.226"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWO31_002936"
     CDS             complement(<1..339)
                     /locus_tag="IWO31_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0303268.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..>451)
                     /locus_tag="IWO31_002937"
     CDS             complement(356..>451)
                     /locus_tag="IWO31_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0303269.1"
                     /translation="FWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          132 a           77 c           74 g          168 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga a
//
LOCUS       DACECS010000216          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.227, whole genome shotgun sequence.
ACCESSION   DACECS010000216 DACECS010000000
VERSION     DACECS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.227"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>441
                     /locus_tag="IWO31_002938"
     CDS             <1..>441
                     /locus_tag="IWO31_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0303270.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSL"
BASE COUNT          194 a           61 c           74 g          112 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg a
//
LOCUS       DACECS010000217          431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.228, whole genome shotgun sequence.
ACCESSION   DACECS010000217 DACECS010000000
VERSION     DACECS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.228"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..412
                     /gene="scm"
                     /locus_tag="IWO31_002939"
     CDS             <1..412
                     /gene="scm"
                     /locus_tag="IWO31_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0303271.1"
                     /translation="TSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTS
                     DSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALP
                     KTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          167 a           93 c           70 g          101 t
ORIGIN      
        1 cacgtcagct tcaagtgaca gtacagatac tacctctacc acaagtgata gctcagattc
       61 atctaccact acaagtgata gcacggacac gtcagcttca agtgacagta cagatacatc
      121 ctctaccaca agtgatagct cagattcatc aacttcaagc gatagtacag acacgtcgtc
      181 taccacaagc gatacacgtc atgcaagtgt ctccttctca aaaacaagtt cggataacag
      241 gacgaacttt aacttaacta ataaaacaag caatgacaat ggaaacaaat caaaaagagc
      301 acttcctaaa actggaagtc aatcgaataa ttggataaca ctcgcaggcg ttatattact
      361 tgtaatcggt ttaagaatca cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat
      421 tcaagataaa a
//
LOCUS       DACECS010000218          429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.229, whole genome shotgun sequence.
ACCESSION   DACECS010000218 DACECS010000000
VERSION     DACECS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.229"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>429
                     /locus_tag="IWO31_002940"
     CDS             <1..>429
                     /locus_tag="IWO31_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303272.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          157 a           75 c           88 g          109 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcaca
//
LOCUS       DACECS010000219          400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.230, whole genome shotgun sequence.
ACCESSION   DACECS010000219 DACECS010000000
VERSION     DACECS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.230"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>400
                     /locus_tag="IWO31_002941"
     CDS             <1..>400
                     /locus_tag="IWO31_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303273.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDR"
BASE COUNT          140 a           84 c           78 g           98 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat
//
LOCUS       DACECS010000220          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.231, whole genome shotgun sequence.
ACCESSION   DACECS010000220 DACECS010000000
VERSION     DACECS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.231"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO31_002942"
     CDS             complement(<1..132)
                     /locus_tag="IWO31_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0303274.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACECS010000221          374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.232, whole genome shotgun sequence.
ACCESSION   DACECS010000221 DACECS010000000
VERSION     DACECS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.232"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>374
                     /locus_tag="IWO31_002943"
     CDS             <1..>374
                     /locus_tag="IWO31_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303275.1"
                     /translation="GQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTE
                     AMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNL
                     LTYEEGITNAMIYPYSNGKIEA"
BASE COUNT          128 a           82 c           68 g           96 t
ORIGIN      
        1 cggacagaaa agccaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg
       61 tgcaaacatc aaccattatg aatacaaaac atggaaaagt ttccgagcac caaaataccc
      121 atttcttact gaagcaatga tgattgatcg attgcttgag ttttctccgc ccctaaagga
      181 ggcgtatccc ttttttcatg aattagttga agcctttcga gacaaagacc ctgacttatt
      241 tttctcctta ttggcagaac ttcccgaaac gttagatgac agctttcggg aaaagcttca
      301 aaaccttctg acctatgaag aaggcatcac caacgcaatg atctatcctt attccaatgg
      361 aaaaatagaa gcga
//
LOCUS       DACECS010000222          371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.233, whole genome shotgun sequence.
ACCESSION   DACECS010000222 DACECS010000000
VERSION     DACECS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.233"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..152)
                     /locus_tag="IWO31_002944"
                     /pseudo
     CDS             complement(1..152)
                     /locus_tag="IWO31_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          128 a           70 c           49 g          124 t
ORIGIN      
        1 gtgatgacgg cacttttatg gtggatgccg acaatcgtat caccctctaa atgtccaaat
       61 tcttcttcga aaacgacata atctttttta cgctcagaaa tatttctttt gaatgcctgt
      121 tttccacgtt tttccttata accatttcat tgtgatccac ctcttttgtt tattagttta
      181 aaccatcaaa ccatttttaa taaaatatgt aaattccttt gctcaaaaaa gtagcttctt
      241 tacgattcga caatttcaaa taaagcaggt aaaaactggc tgtcagaaaa tagccaaact
      301 gaagcaaaaa ataatgttaa aattaattaa tgaacgaaaa aaatccctca cctaatatta
      361 cggtgaggaa t
//
LOCUS       DACECS010000223          365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.234, whole genome shotgun sequence.
ACCESSION   DACECS010000223 DACECS010000000
VERSION     DACECS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.234"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>365)
                     /locus_tag="IWO31_002945"
     rRNA            complement(<1..>365)
                     /locus_tag="IWO31_002945"
                     /product="23S ribosomal RNA"
BASE COUNT           80 a          120 c           72 g           93 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccagcag tcctcacgat
      241 ctgccttcaa cgcccttaga acgctctcct accaatacac ctaaaggtgt actccacagc
      301 ttcggtaata tgtttagccc cggtacattt tcggcgcagg gtcactcgac tagtgagcta
      361 ttacg
//
LOCUS       DACECS010000224          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.denovo.235, whole genome shotgun sequence.
ACCESSION   DACECS010000224 DACECS010000000
VERSION     DACECS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.denovo.235"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          112 a           81 c           57 g          112 t
ORIGIN      
        1 gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta atactcgaaa
       61 ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
      121 ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt
      181 gatgcagggc agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg
      241 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
      301 tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc
      361 ga
//
LOCUS       DACECS010000225        10208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.088, whole genome shotgun sequence.
ACCESSION   DACECS010000225 DACECS010000000
VERSION     DACECS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.088"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            296..571
                     /locus_tag="IWO31_002946"
     CDS             296..571
                     /locus_tag="IWO31_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303276.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            584..2656
                     /locus_tag="IWO31_002947"
     CDS             584..2656
                     /locus_tag="IWO31_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0303277.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2821..4707
                     /locus_tag="IWO31_002948"
     CDS             2821..4707
                     /locus_tag="IWO31_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0303278.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4719..5666
                     /locus_tag="IWO31_002949"
     CDS             4719..5666
                     /locus_tag="IWO31_002949"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0303279.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5686..6213
                     /locus_tag="IWO31_002950"
     CDS             5686..6213
                     /locus_tag="IWO31_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0303280.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6276..6929)
                     /locus_tag="IWO31_002951"
     CDS             complement(6276..6929)
                     /locus_tag="IWO31_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0303281.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7061..7903
                     /locus_tag="IWO31_002952"
     CDS             7061..7903
                     /locus_tag="IWO31_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0303282.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8061..8957
                     /locus_tag="IWO31_002953"
     CDS             8061..8957
                     /locus_tag="IWO31_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0303283.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8960..9673
                     /locus_tag="IWO31_002954"
     CDS             8960..9673
                     /locus_tag="IWO31_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0303284.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9693..>10208
                     /gene="msrA"
                     /locus_tag="IWO31_002955"
     CDS             9693..>10208
                     /gene="msrA"
                     /locus_tag="IWO31_002955"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0303285.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
BASE COUNT         3547 a         1741 c         2114 g         2806 t
ORIGIN      
        1 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
       61 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      121 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      181 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      241 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      301 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      361 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      421 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      481 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      541 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      601 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      661 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      721 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      781 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      841 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      901 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
      961 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1021 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1081 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1141 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1201 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1261 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1321 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1381 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1441 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1501 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1561 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1621 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1681 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1741 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1801 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1861 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1921 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     1981 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2041 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2101 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2161 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2221 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2281 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2341 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2401 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2461 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2521 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2581 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2641 aaaacgagtc caataaaata aaaaggtaaa aattttcaag ttttataaaa aacaaaacat
     2701 atcagaacag gatcgaatcg gtcctgttct ttttttgaga acacaaaaag aacaaatacc
     2761 aaaaaaattt tcctgaagtt cactattttt atgatacaat atccaagata aggagggaac
     2821 atgttgaaag aattaaaagt aactgacctc aaaaaaactt atggtgaaaa agatttgttt
     2881 gaccagattt cttttttggt ccacgaaaaa gaccgtatcg ggttgattgg gacgaacggg
     2941 acgggtaaaa caagtctctt aaatattatt gccggtatcg atagcagcga cggtgatcgg
     3001 caaacagtgt tttatccaac tgattaccgt attggttatc tttctcaagc agctgaattt
     3061 tccgatgaat taactgttct ccaagcagtc tttcaaggaa acagcccctt gatccaaact
     3121 gttcgagcat atgaggaagc attgatcgaa ctaggggaaa acggagaaga ccctgatgtg
     3181 caaaaacgtt atgctaaagc agaagaacaa atgaataaag aagatgcttg gactacagat
     3241 accaacgcaa agattatttt gcaaaaatta ggaatcagtg aattagataa aaaaatcagt
     3301 actttatcag gtggtcagaa aaaacgagtt agtctggcgc aagtgttgat tgatgaaccc
     3361 gatttactat tactagacga accaacaaac catttagatt atgaagcgat cgagtggttg
     3421 gaaaactatt tgaaacaata ccgtggcgca ctattgatgg ttacccacga tcgttatttt
     3481 ctagatagag tagcaaaccg aatcttcgaa ctttcatttg gaaaattata cgagtataaa
     3541 ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg atcgtgtaga agtagaacaa
     3601 gaagaaaagc gcaagcggct ttataaacag gaattagctt ggatgagggc tggcgtacag
     3661 gcacgtggca ccaagcagca agcacggatc aaccgtttcg aagatctaaa ggagaacttg
     3721 taccaagtaa accaagaaga tgatttagag ctgaacctag cgacacaacg tttagggaaa
     3781 aaagtactgg aaatcaaaga tggcagttac cgtataaatg accaaacact gctagaacat
     3841 ttagatttat tgattcaatc tcgggaaaga ctaggtatta ctggtaaaaa tggtgcggga
     3901 aaatcgacat tgcttaatat tctagctggc agaattcccc tggatagcgg tacaatgtct
     3961 atcggtgaaa cggttcatct tgcgtattat acacaagaaa atgaagaaat ggcaccagat
     4021 aaacggatga ttgcttactt acaagaagct gctgaagaag caaaaacagc agatggcagt
     4081 caaatcggag tagcagaatt acttgaacga tttttatttc caagatttat gcatgggaca
     4141 ctgataggca aactttcagg cggagaaaaa cgccggttat ttttgttgaa actgctgatt
     4201 cagcaaccta atgttttatt attggatgaa ccgaccaatg atttggacat agcaacattg
     4261 acgattttgg aagattattt tagaagtttt ccaggtgctg tgatcactgt ttcccatgac
     4321 cgttatttct tggacaaggt agcagataag ttattagtat tccaaggaaa cggaaaacaa
     4381 gaactttatt acggaaacat gtccagttat ttactgaagc aaaaagagac ccagcagcct
     4441 gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg ctgaaaagaa gaaattatct
     4501 tacatggaac aaaaagaatg ggaaacgatt gaagatgaga ttgcagaact tgaagaaaaa
     4561 atctcattgc tccaagaaga gatgaatcat caaggagata atttcactcg attacaagaa
     4621 ttgcaaaacg acgtatctga aacagaagca caacttgaag aaaaaatggc acggtgggaa
     4681 tacttgagtg aatgggtaga agattaggag gaaaaaggat ggaacaagcg tatcttgact
     4741 taggaagaaa attgttggaa gaagggcatc gtaaagaaga cagaacaggc acaggcacat
     4801 taagcttatt cggataccaa atgcggtttg atctgcaaaa agggtttcct ttgttaacaa
     4861 cgaaacgagt accttttgga ttgatcaaaa gcgaattgct ttggttttta aaaggagata
     4921 cgaatatccg ctatctatta caaaataaaa accatatttg ggatgaatgg gcatttgaac
     4981 gctacatcaa aagtccagac tataatggac cagatatgac tgattttggc catagatgtt
     5041 tgacagatcc tgaattcaat gaagaatata aaaagcagag caagcttttt tgcgagaaga
     5101 tcctaaccga tgattccttt gctgaaacat acggcgatct tggacacatc tacggctatc
     5161 aatggcgtca ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa caagtgatcg
     5221 aagcaatcaa aaagactcct gattctagaa gattgatcgt ttctgcatgg aatccggaag
     5281 atgttcctag catggctttg cctccttgtc atacgatgtt tcaattttat gttcaagagg
     5341 gacggctaag ctgccaattg tatcaacgca gtggggatgt gtttctaggc gttccgttca
     5401 acattgcaag ttatgcgttg ctgactcacc taattgcaca tgaaacaggt ttagcggttg
     5461 gcgaatttgt tcacacatta ggagatgctc atctttatat caatcatatc gaacaaatga
     5521 aagaacaatt gagccgagaa atacgcgcat ttccgacttt agttttgaat caggaaaagc
     5581 agtcagtatt tgattttgag atggaagata tccaattaga aggatatgac ccacatccgg
     5641 cgatcaaagc tcctatcgct gtttagaatc agaaaggatg agaaaatgtt agctgcaatt
     5701 tgggcacagg atgaaaacgg attgattgga aaagaagacc gtcttccttg gcgtttgcca
     5761 aatgatctga aattcttcaa acaaatgaca gaagcaaata cactagtgat gggacgaaaa
     5821 acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt tttgacgaga
     5881 gattcttcct ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga cgtgcttgct
     5941 ctggaaaaag aaacagacgg tatctttttc atcgctggag ggtcagctgt ttatcaagaa
     6001 tttctgccat tttgtacgat attataccgt acagtcatcc atcatgtatt cgatggagat
     6061 gcttattttc cacctgttga ctggtctgat tggtcactga tcaatactag tcaaggagaa
     6121 atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa tgagaatcct
     6181 gtaaaaagcg ggacaggcga attgacgata taaaaaagct atggatagac tagctgttat
     6241 ttagagctgt cccatccata gctttatttt ttttattaag taaagaataa aacagagaag
     6301 tacataaaac ctgcacctaa cataacaaac aaatgccaaa caacatgcat atagcggata
     6361 gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa gcccgcctgc taccatcaaa
     6421 ataacaccag tcattccaag agagtgataa agaggaacag cagccacgac acagagccac
     6481 cccataaaaa tatagatgat cgtagatact tttgatactc tctcccgttt atgcaacgtc
     6541 aacgctttat atataatgcc aagaatcgca aacaaccaga ttaatacaaa taaagtccaa
     6601 cctagccatc ctcggatact cagcaaacaa aaaggagtat aacttcctgc aatcaaaaga
     6661 aaaatcgaac tatgatcgaa tacttggaac accttttttg cttttgtaaa aatcaatgaa
     6721 tgaaacaaag tagacgttaa aaataaaaga atcatcatcg atccatagat agcataagag
     6781 acaatatgga gggaggaatg caagtgggca ccttttacta aaagaaccac tagtcccgcg
     6841 atactcaatc cagtcccaat tccatgggtg actgcattaa acacttcatt taatatttca
     6901 tacgttcgat taaatttcgt tttttccatg aaaacggctc ctttactagg tgtaattaat
     6961 gaaaaatcat tatttgtctg ttatactaaa aatagaaatt gtcttacatg tagtttaaca
     7021 tgaataaggc atcttgttaa agaaagagag agagaaaata atgacaaatg taaaaatcgt
     7081 aacagattct tcatgtacga tggaaaaaaa tgtacgcgat gctttaggca ttcgtgtcat
     7141 gccgctatcc atcatgatcg acggaacagt atatcccgat gatgaccaac tagacggcga
     7201 aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc aaaacaagcc aaccaccaat
     7261 tggagaattc atctcgatgt atgatgagct aggagaagac ggcagtgaag tgatttcgat
     7321 ccatatgaca aaaggcttga gtggaacagt agaagcagca agacaagcca gtaatctaac
     7381 aaaaacgaaa gtaacggtta tcgacagtga taccactgac caaggattat cttttcaggt
     7441 aattcgagca gctgaattag caaaagaagg taaaacagta gagaaaatcc ttgaagaagt
     7501 caaaaaagta caagacaata caaaattata catcggaata tccacactag ataacttggt
     7561 aaaaggtgga agaatcagcc gaacaactgg tttgctgtct agtgttttga acatacgcgt
     7621 ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact aaggggcgag gaaagaaaac
     7681 gttcgataaa tggtttaata atttgaaaaa tgaactgaaa gaattgccaa atgtgcgaca
     7741 aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa gaattcaaag agggtttgca
     7801 agaattattc ccggacatgc atatccctgt actgcatacg aatccggtta tcgctacgca
     7861 tactggtaaa gacgcgtttg caatcatgta ttatgtagat tagtcgaaaa gaggataagc
     7921 cctcagcctg aaaggcttgc agcttattct ctttttcttt ataaaattag gaaaataatt
     7981 gttttctttt ttcataaaga ctaccttttt gataaaattt ttgataacat tagaaccaat
     8041 cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca tttgatcctc gttttgttcc
     8101 cattattaat tgcgatcatc atctatgcag gtttagctgc ttctataccg aaagctggcc
     8161 cactaatgaa accagtaact actgtgagag aagatccaaa taaaaaagaa aaacttcact
     8221 acatagctat cggagattcg ctgactgaag gagtaggaga tcaaaccaag caaggaggat
     8281 tcgttcccct tgtcgcaaat gatctaaaag accgttataa tttgaccgcg gttgaaacag
     8341 aaaactatgg tgtaaatggt gaacgaagtg accagatctt aaagcgagta aaaacaaaag
     8401 aagaaattcg aaacaatatc aaaacagctg attttatcac actgactgtt ggcggtaatg
     8461 atttgatgaa agttatccaa aataatttat tcgggttaac ggtcaattcc tttaaaaagc
     8521 cactgaaaaa ctatcaggaa aatgtcacag aactattgga agaatttcga tccctcaata
     8581 aaaccgcacc tatttacatt ttaggcatct acaatccatt ttatttgaac tttcctgaga
     8641 tcactgacat gcagacaatt gttaacaatt ggaacaaagg aacagaaacg gtgacaaaaa
     8701 aaatgccgaa ctgctacttt atcccaatca atgatttgct ttatcaagga ttgaatcagg
     8761 aagtaggtgt cacagaagaa ggaacagatc aaacaactag cacgacagaa aatctaaatg
     8821 gcatcaaaaa taatgtgctc tatgaagaag atcatttcca tccaaataat ttaggttatc
     8881 aattgatggc aaatgcagtg agagatcagc tgatcgagac acaaaaccaa tggttagaga
     8941 aggaggatga tcaataaaca tgaaaacaag atctgacgta tcttcaaaca aaacaaagaa
     9001 aacaaattac tggaaattcg ccttccttat ccttcttggg gtagtacttg gttcgaccgt
     9061 atttttaggc agccgtattt ttgctaatcg ggaaccagaa ctcccagaaa taccagctct
     9121 tacggaaaga caaggagatc ctgtcttaac gatcaattca aataaagaaa aagtcaatca
     9181 aatcatcagc tttttcctta gtgagtatca aaaagatagt gatattgaat ataaatttta
     9241 tttggaaaat gaagcactac tcaatggtac tttcgaagta ctaggttttc ctatcgattt
     9301 ctatttatat tttgatccgt atgtgatgga aaacggaaat gtccaactaa aagctagaag
     9361 tctttctatc ggaacattga gtttgccaat caaagatgtg atgaatatga tcaagagaaa
     9421 ctataaatta ccagattgga tcgaagtcaa tacagatgat ctgactatca tgcttaggtt
     9481 ggatcaattc cgaatgcaaa acgggatgta tattaaagca gataaaatcg acttagtaaa
     9541 tgatgacatt cggtttagtc tatatttgcc aaaagatacc tctgatgctt caaacaactc
     9601 aaataccaac aaaactagca ccagcagtac tgaaagcaag acaaaagaaa ataaaaaatc
     9661 atcggatgag tagtattaga gggggaatca taatggaacg agcaatattt gcaggaggct
     9721 gcttctggtg tatgatccag cctttcgaca cgcttcctgg tatccataca gtgatgtcag
     9781 gatatactgg agggcatgta ccagatccga cttatgaaca agtaaaaagc caaacgactg
     9841 gacatacaga agcagtggag attatctatg atccaacatt gatttcatat gaacaattag
     9901 tagaacttta ctggcagcaa actgatccaa ctgacgcttt cggacaattc gaggatcgcg
     9961 gtgacaatta tcgcccggtg atcttttatt tgacagaaga acagcggatc attgctgaaa
    10021 aaagtaagaa acagttagct gaaagtggac gtttcgaaca gccaatcgtt acaaaaatcg
    10081 aaccagccca accattttat ctagcagaag aagaccatca agagtattat aaaaagcatc
    10141 ctgaaaattt taaaagaaac catgcacgcc gggctgcctt tatcgaagaa aattggagga
    10201 acgaaggg
//
LOCUS       DACECS010000226         5700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.117, whole genome shotgun sequence.
ACCESSION   DACECS010000226 DACECS010000000
VERSION     DACECS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.117"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..239
                     /locus_tag="IWO31_002956"
                     /pseudo
     CDS             1..239
                     /locus_tag="IWO31_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            370..1455
                     /locus_tag="IWO31_002957"
     CDS             370..1455
                     /locus_tag="IWO31_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0303286.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1452..3344
                     /locus_tag="IWO31_002958"
     CDS             1452..3344
                     /locus_tag="IWO31_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0303287.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3351..3728
                     /locus_tag="IWO31_002959"
     CDS             3351..3728
                     /locus_tag="IWO31_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0303288.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3882..4664
                     /locus_tag="IWO31_002960"
     CDS             3882..4664
                     /locus_tag="IWO31_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAQ0303289.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4790..5521)
                     /gene="erm(A)"
                     /locus_tag="IWO31_002961"
     CDS             complement(4790..5521)
                     /gene="erm(A)"
                     /locus_tag="IWO31_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ0303290.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5579..5700)
                     /locus_tag="IWO31_002962"
                     /pseudo
     CDS             complement(5579..5700)
                     /locus_tag="IWO31_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554414.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
BASE COUNT         2057 a          785 c         1090 g         1768 t
ORIGIN      
        1 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
       61 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
      121 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
      181 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
      241 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
      301 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
      361 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
      421 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
      481 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
      541 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
      601 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
      661 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
      721 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
      781 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
      841 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
      901 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
      961 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
     1021 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
     1081 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
     1141 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
     1201 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
     1261 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
     1321 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
     1381 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
     1441 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
     1501 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
     1561 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
     1621 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
     1681 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
     1741 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
     1801 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
     1861 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
     1921 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
     1981 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
     2041 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
     2101 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
     2161 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
     2221 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
     2281 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
     2341 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
     2401 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
     2461 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
     2521 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
     2581 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
     2641 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
     2701 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
     2761 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
     2821 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
     2881 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
     2941 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
     3001 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
     3061 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
     3121 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
     3181 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
     3241 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
     3301 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
     3361 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
     3421 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
     3481 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
     3541 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
     3601 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
     3661 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
     3721 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
     3781 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
     3841 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
     3901 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
     3961 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
     4021 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
     4081 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
     4141 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
     4201 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
     4261 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
     4321 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
     4381 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
     4441 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
     4501 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
     4561 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
     4621 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
     4681 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
     4741 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
     4801 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
     4861 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
     4921 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
     4981 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
     5041 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
     5101 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
     5161 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
     5221 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
     5281 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
     5341 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
     5401 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
     5461 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
     5521 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
     5581 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
     5641 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
//
LOCUS       DACECS010000227         5045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.119, whole genome shotgun sequence.
ACCESSION   DACECS010000227 DACECS010000000
VERSION     DACECS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.119"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            102..1223
                     /locus_tag="IWO31_002963"
     CDS             102..1223
                     /locus_tag="IWO31_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0303291.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1481..2983
                     /locus_tag="IWO31_002964"
     CDS             1481..2983
                     /locus_tag="IWO31_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0303292.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3050..3577
                     /locus_tag="IWO31_002965"
     CDS             3050..3577
                     /locus_tag="IWO31_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0303293.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3788..4849
                     /locus_tag="IWO31_002966"
     CDS             3788..4849
                     /locus_tag="IWO31_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0303294.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4852..>5045
                     /gene="murQ"
                     /locus_tag="IWO31_002967"
                     /gene_synonym="yfeU"
     CDS             4852..>5045
                     /gene="murQ"
                     /locus_tag="IWO31_002967"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0303295.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKG"
BASE COUNT         1745 a          856 c         1108 g         1336 t
ORIGIN      
        1 tttttatttt aaatggctaa gttcattata aaatccgcca ttgttttgtt tgaaaaaaat
       61 gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa
      121 ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct
      181 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
      241 cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct
      301 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag
      361 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
      421 tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag
      481 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
      541 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
      601 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
      661 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
      721 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
      781 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
      841 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
      901 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
      961 ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa
     1021 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
     1081 tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
     1141 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     1201 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     1261 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     1321 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     1381 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     1441 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     1501 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     1561 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     1621 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     1681 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     1741 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     1801 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     1861 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     1921 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     1981 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     2041 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     2101 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     2161 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     2221 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     2281 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     2341 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     2401 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     2461 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     2521 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     2581 tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga
     2641 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     2701 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     2761 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     2821 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     2881 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     2941 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     3001 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     3061 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     3121 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     3181 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     3241 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     3301 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     3361 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     3421 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     3481 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     3541 catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat
     3601 aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt
     3661 ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa
     3721 attttgtttc tttagatata tactctagtc atagaaatga agacggatac agaaaaagag
     3781 ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag agaagaaaca
     3841 gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca tgcatatacc
     3901 agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa
     3961 gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag cgggtttttc
     4021 attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa tggattatca
     4081 tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt tgaatgctag
     4141 cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt tctcacagat
     4201 tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag aatggtatca
     4261 gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg taccaggaga
     4321 tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac atcgagggat
     4381 ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg tttacatcgg
     4441 agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa aagaacagac
     4501 agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat tagggaagca
     4561 tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac gttttcgaga
     4621 gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta ttactttaga
     4681 taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg atttgccagc
     4741 ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat tgatcaacca
     4801 aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa aatgagcaaa
     4861 atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg attagatgag
     4921 atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt ggccgctgcg
     4981 gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga gtcctttaaa
     5041 aaagg
//
LOCUS       DACECS010000228         4999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.120, whole genome shotgun sequence.
ACCESSION   DACECS010000228 DACECS010000000
VERSION     DACECS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.120"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            405..869
                     /locus_tag="IWO31_002968"
     CDS             405..869
                     /locus_tag="IWO31_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0303296.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            853..1482
                     /locus_tag="IWO31_002969"
     CDS             853..1482
                     /locus_tag="IWO31_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0303297.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1836..3314
                     /gene="msr"
                     /locus_tag="IWO31_002970"
     CDS             1836..3314
                     /gene="msr"
                     /locus_tag="IWO31_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0303298.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3361..3807
                     /locus_tag="IWO31_002971"
     CDS             3361..3807
                     /locus_tag="IWO31_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0303299.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4006..4521)
                     /locus_tag="IWO31_002972"
     CDS             complement(4006..4521)
                     /locus_tag="IWO31_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0303300.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4638..>4999
                     /locus_tag="IWO31_002973"
     CDS             4638..>4999
                     /locus_tag="IWO31_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0303301.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWG"
BASE COUNT         1611 a          833 c         1033 g         1522 t
ORIGIN      
        1 gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag gttgtgacaa
       61 catcttgtca taactttttt attactgttt aagcatactg atccaatttc taatattcga
      121 cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaatta tctactgcag
      181 aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg ttgtgctttc
      241 tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt ttctattgag
      301 agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat tctattattg
      361 acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga
      421 ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag
      481 aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc
      541 cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc
      601 atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc
      661 gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcaagcat tgtgaaatgg
      721 accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat
      781 ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag
      841 aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc
      901 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
      961 gaagcgacga ctgcaccttc ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa
     1021 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
     1081 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
     1141 atctacaacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
     1201 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
     1261 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
     1321 acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa
     1381 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
     1441 tctgttcgtt tagcaccaga aaagatcact atgttcgaat aaaaggtttt cagttactat
     1501 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
     1561 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
     1621 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
     1681 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
     1741 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
     1801 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     1861 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     1921 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     1981 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     2041 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     2101 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     2161 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     2221 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     2281 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     2341 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     2401 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     2461 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     2521 tgtccagtaa aaagaaaaag caacaaatca gactggatcg gttgtcttcc tctaaacaaa
     2581 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     2641 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     2701 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     2761 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     2821 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     2881 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     2941 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     3001 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     3061 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     3121 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     3181 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     3241 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     3301 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     3361 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     3421 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     3481 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     3541 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc
     3601 ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg
     3661 atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg
     3721 aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg
     3781 ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca
     3841 tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt
     3901 tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat
     3961 aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg
     4021 ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct
     4081 gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa
     4141 ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc
     4201 agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt
     4261 ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc
     4321 agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc
     4381 ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat
     4441 tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa
     4501 tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca
     4561 cagtaaagtt tccacgtttt acatgtgcat attgacatgt aaaataaatc atgtatgatg
     4621 ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa
     4681 atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt
     4741 ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta
     4801 cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc
     4861 tatttacagg tttcttatct agtcggatcg gtttgaaaaa aacattgatg acaggattgt
     4921 ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga atgctgattg
     4981 ggttacgtgc cggctgggg
//
LOCUS       DACECS010000229         4071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.137, whole genome shotgun sequence.
ACCESSION   DACECS010000229 DACECS010000000
VERSION     DACECS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.137"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            74..190
                     /locus_tag="IWO31_002974"
     CDS             74..190
                     /locus_tag="IWO31_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0303302.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            206..2125
                     /gene="tet(M)"
                     /locus_tag="IWO31_002975"
     CDS             206..2125
                     /gene="tet(M)"
                     /locus_tag="IWO31_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0303303.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVE
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2235..2285
                     /locus_tag="IWO31_002976"
                     /pseudo
     CDS             2235..2285
                     /locus_tag="IWO31_002976"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2319..3695
                     /gene="tet(L)"
                     /locus_tag="IWO31_002977"
     CDS             2319..3695
                     /gene="tet(L)"
                     /locus_tag="IWO31_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0303304.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKSPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGALIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPTITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIMVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            complement(3744..3944)
                     /locus_tag="IWO31_002978"
     CDS             complement(3744..3944)
                     /locus_tag="IWO31_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0303305.1"
                     /translation="MPIFILSVLSSLPKARLSKNKIFIVFRFSSVTDKTTQNKKDTRE
                     VSRIFYSAIAPFNGTKRFEFLL"
BASE COUNT         1292 a          624 c          830 g         1325 t
ORIGIN      
        1 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
       61 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
      121 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
      181 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
      241 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
      301 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
      361 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
      421 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
      481 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
      541 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
      601 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctatggaaat
      661 tataatcaaa cagaaagtag agctgcaccc taatatgtgt gtgatgagct gtacggaacc
      721 tgagcaatgg gatgtggtaa tagaaggaaa tgatgacctt ttagagaaat atatgtccgg
      781 taaatcatta gaagcattag aactcgaaca agaggaaatc agaagatttc agaattgctc
      841 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
      901 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
      961 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatag
     1021 tggagtacta catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     1081 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     1141 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     1201 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     1261 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     1321 cccgcttcta caatattatg tggattctac gacacatgaa atcatacttt ctttcttagg
     1381 gaaagtacaa atggaagtga cttgtgctct attgcaagaa aagtatcatg tggaggtaaa
     1441 aataaaaaag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
     1501 tcacatcgaa gtgccaccga atcccttctg ggcttccatt ggtctttctg tagaaccgct
     1561 tccattaggg agcggagtac agtatgagag ctcggtttct cttggatact taaatcaatc
     1621 gtttcaaaat gcagttatgg aagggatacg atatggctgt gaacaaggat tgtatggttg
     1681 gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     1741 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     1801 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     1861 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     1921 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     1981 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     2041 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     2101 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     2161 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     2221 ttatgaagtg aaatgaatgt aacagggttc aattaaaaga gggaagcgta tcattaaccc
     2281 tataaactac gtctgccctc attattggag ggtgaaatgt gaatacatcc tattcacaat
     2341 cgaatttacg acacaaccaa attttaattt ggctttgcat tttatctttt tttagcgtat
     2401 taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt aataaatcac
     2461 ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt ggaacagctg
     2521 tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt ggaattataa
     2581 taaattgttt cggggcgtta attgggtttg ttggccattc tttcttttcc ttacttatta
     2641 tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta atggttgtag
     2701 ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt ggatcgatag
     2761 tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat tatattcatt
     2821 ggtcctatct tctactcatt cctacgataa caattatcac tgttccgttt cttatgaaat
     2881 tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt atactaatgt
     2941 ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct tttcttatcg
     3001 ttagcgtgct gtcattcctg atatttgtaa aacatatcag gaaagtaaca gatccttttg
     3061 ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt gggggaatta
     3121 tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa gatgtttacc
     3181 agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg agtgtcatta
     3241 ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac gtgttaaaca
     3301 tcggagttac atttctttct gttagctttt taactgcttc ctttctttta gaaacaacat
     3361 catggttcat gacaattata atggtatttg ttttaggtgg gctttcgttc accaaaacag
     3421 ttatatcaac aattgtttca agtagcttga aacagcagga agctggtgct ggaatgagtt
     3481 tgcttaactt taccagcttt ttatcagagg gaacaggtat tgcaattgta ggtggtttat
     3541 tatccatacc cttacttgat caaaggttgt tacctatgga agttgatcag tcaacttatc
     3601 tgtatagtaa tttgttatta cttttttcag gaatcattgt cattagttgg ctggttacct
     3661 tgaatgtata taaacattct caaagggatt tctaaatcgt taagggatca actttgggag
     3721 agagttcaaa attgatcctt tttttataac aggaattcaa atctttttgt tccattaaag
     3781 ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt attttgagtg
     3841 gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt gctgagtctg
     3901 gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt aaaggtggag
     3961 attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt taaacgagca
     4021 cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt t
//
LOCUS       DACECS010000230         2521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.151, whole genome shotgun sequence.
ACCESSION   DACECS010000230 DACECS010000000
VERSION     DACECS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.151"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWO31_002979"
     CDS             complement(<1..164)
                     /locus_tag="IWO31_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0303306.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IWO31_002980"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IWO31_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0303307.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..>2521)
                     /locus_tag="IWO31_002981"
     CDS             complement(963..>2521)
                     /locus_tag="IWO31_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0303308.1"
                     /translation="VVEEIKRLIEINSIQDVEPDYQFHKEGCSEEESTPFNEPKQFYK
                     RGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPMFAKMVVGRVSVSRTCD
                     ILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWLNTDKMHYYLNNVRKKG
                     QGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISMDELNEDTRKFKEDHLN
                     TFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHY
                     WLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKE
                     FGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNP
                     IKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERP
                     LTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQ
                     FDWKDIIYFK"
BASE COUNT          799 a          410 c          439 g          873 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 g
//
LOCUS       DACECS010000231         2450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.154, whole genome shotgun sequence.
ACCESSION   DACECS010000231 DACECS010000000
VERSION     DACECS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.154"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(181..1284)
                     /locus_tag="IWO31_002982"
     CDS             complement(181..1284)
                     /locus_tag="IWO31_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0303309.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1837..2385
                     /gene="aac(6')"
                     /locus_tag="IWO31_002983"
     CDS             1837..2385
                     /gene="aac(6')"
                     /locus_tag="IWO31_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0303310.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          787 a          430 c          473 g          760 t
ORIGIN      
        1 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
       61 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
      121 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
      181 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
      241 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
      301 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
      361 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
      421 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
      481 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
      541 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
      601 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
      661 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
      721 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
      781 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
      841 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
      901 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
      961 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
     1021 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
     1081 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
     1141 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
     1201 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
     1261 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
     1321 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
     1381 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
     1441 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
     1501 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
     1561 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
     1621 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
     1681 tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga
     1741 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
     1801 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
     1861 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
     1921 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
     1981 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
     2041 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
     2101 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
     2161 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
     2221 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
     2281 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
     2341 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
     2401 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACECS010000232         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.168, whole genome shotgun sequence.
ACCESSION   DACECS010000232 DACECS010000000
VERSION     DACECS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.168"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..416
                     /locus_tag="IWO31_002984"
     CDS             24..416
                     /locus_tag="IWO31_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0303311.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWO31_002985"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWO31_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ0303312.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACECS010000233         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.179, whole genome shotgun sequence.
ACCESSION   DACECS010000233 DACECS010000000
VERSION     DACECS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.179"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IWO31_002986"
     CDS             <1..1311
                     /locus_tag="IWO31_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0303313.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DACECS010000234         1366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.180, whole genome shotgun sequence.
ACCESSION   DACECS010000234 DACECS010000000
VERSION     DACECS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.180"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            189..272
                     /locus_tag="IWO31_002987"
     CDS             189..272
                     /locus_tag="IWO31_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0303314.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            397..1134
                     /gene="erm(B)"
                     /locus_tag="IWO31_002988"
     CDS             397..1134
                     /gene="erm(B)"
                     /locus_tag="IWO31_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0303315.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          511 a          215 c          219 g          421 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg tcttgcgtat ggttaaccct
       61 aaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt gcattaaaaa
      121 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
      181 gattcgtcat gttggtattc caaatgcgtt atgtagataa aacatctact gttttgaaac
      241 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      301 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      361 tttgtttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      421 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      481 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      541 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      601 gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      661 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      721 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      781 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      841 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      901 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      961 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
     1021 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
     1081 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1141 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1201 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1261 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1321 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgtttt
//
LOCUS       DACECS010000235         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20305_2017
            SAMN12639954-rid9879943.guided.194, whole genome shotgun sequence.
ACCESSION   DACECS010000235 DACECS010000000
VERSION     DACECS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639954
            Sequence Read Archive: SRR10023232
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741757.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741757.1
            Genome Coverage       :: 255x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:12:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,989
            CDSs (total)                      :: 2,935
            Genes (coding)                    :: 2,858
            CDSs (with protein)               :: 2,858
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 32 of 77
            Pseudo Genes (incomplete)         :: 36 of 77
            Pseudo Genes (internal stop)      :: 20 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639954-rid9879943.guided.194"
                     /isolate="E20305_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            9..1049
                     /locus_tag="IWO31_002989"
     CDS             9..1049
                     /locus_tag="IWO31_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0303316.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          343 a          169 c          214 g          325 t
ORIGIN      
        1 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
       61 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      121 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      181 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      241 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      301 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      361 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      421 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      481 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      541 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
      601 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
      661 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
      721 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
      781 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
      841 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
      901 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
      961 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
     1021 taagatatat gttcaataaa ataacttagt g
//
